keyword
https://read.qxmd.com/read/38536895/the-read-through-transcription-mediated-autoactivation-circuit-for-virulence-regulator-expression-drives-robust-type-iii-secretion-system-2-expression-in-vibrio-parahaemolyticus
#1
JOURNAL ARTICLE
Dhira Saraswati Anggramukti, Eiji Ishii, Andre Pratama, Mohamad Al Kadi, Tetsuya Iida, Toshio Kodama, Shigeaki Matsuda
Vibrio parahaemolyticus is the leading cause of seafood-borne gastroenteritis in humans worldwide. The major virulence factor responsible for the enteropathogenicity of this pathogen is type III secretion system 2 (T3SS2), which is encoded on the 80-kb V. parahaemolyticus pathogenicity island (Vp-PAI), the gene expression of which is governed by the OmpR-family transcriptional regulator VtrB. Here, we found a positive autoregulatory feature of vtrB transcription, which is often observed with transcriptional regulators of bacteria, but the regulation was not canonically dependent on its own promoter...
March 2024: PLoS Pathogens
https://read.qxmd.com/read/38535888/characteristics-of-pathogenic-escherichia-coli-associated-with-diarrhea-in-children-under-five-years-in-northwestern-ethiopia
#2
JOURNAL ARTICLE
Berihun Mossie Mulu, Mequanint Addisu Belete, Tiliksew Bialfew Demlie, Habtamu Tassew, Tesfaye Sisay Tessema
Diarrheagenic Escherichia coli (DEC) are the leading cause of infectious diarrhea and pose a significant global, regional, and national burden of disease. This study aimed to investigate the prevalence of six DEC pathotypes in children with diarrhea and determine their antibiotic resistance patterns. Samples from 107 diarrheagenic children were collected and processed for Escherichia coli ( E. coli ). Single-plex PCR was used to detect target virulence genes as well as characterize and categorize DEC pathotypes...
March 21, 2024: Tropical Medicine and Infectious Disease
https://read.qxmd.com/read/38535842/neonatal-calf-diarrhea-and-gastrointestinal-microbiota-etiologic-agents-and-microbiota-manipulation-for-treatment-and-prevention-of-diarrhea
#3
REVIEW
Emma Jessop, Lynna Li, David L Renaud, Adronie Verbrugghe, Jennifer Macnicol, Lisa Gamsjäger, Diego E Gomez
Neonatal calf diarrhea is the leading cause of neonatal morbidity and mortality globally. The changes associated with the gastrointestinal microbiota in neonatal calves experiencing diarrhea and its etiology are not fully understood or completely defined in the literature. Several studies have demonstrated that the fecal microbiota of calves that experience diarrhea substantially deviates from that of healthy age-matched calves. However, one key question remains: whether the changes observed in the bacterial communities (also known as dysbiosis) are a predisposing factor for, or the consequence of, gastrointestinal inflammation caused by the pathogens associated with calf diarrhea...
February 29, 2024: Veterinary Sciences
https://read.qxmd.com/read/38532960/-shigella-detection-and-molecular-serotyping-with-a-customized-taqman-array-card-in-the-enterics-for-global-health-efgh-shigella-surveillance-study
#4
JOURNAL ARTICLE
Jie Liu, Paul F Garcia Bardales, Kamrul Islam, Sheikh Jarju, Jane Juma, Chimwemwe Mhango, Queen Naumanga, Sonia Qureshi, Catherine Sonye, Naveed Ahmed, Fatima Aziz, Md Taufiqur Rahman Bhuiyan, Mary Charles, Nigel A Cunliffe, Mahamadou Abdou, Sean R Galagan, Ensa Gitteh, Ibrehima Guindo, M Jahangir Hossain, Abdoulie M J Jabang, Khuzwayo C Jere, Flywell Kawonga, Mariama Keita, Noumou Yakhouba Keita, Karen L Kotloff, Wagner V Shapiama Lopez, Stephen Munga, Maribel Paredes Olortegui, Richard Omore, Patricia B Pavlinac, Firdausi Qadri, Farah Naz Qamar, S M Azadul Alam Raz, Laura Riziki, Francesca Schiaffino, Suzanne Stroup, Sarata Nassoun Traore, Tackeshy Pinedo Vasquez, Mohammad Tahir Yousafzai, Martin Antonio, Jennifer E Cornick, Furqan Kabir, Farhana Khanam, Margaret N Kosek, John Benjamin Ochieng, James A Platts-Mills, Sharon M Tennant, Eric R Houpt
BACKGROUND: Quantitative polymerase chain reaction (qPCR) targeting ipaH has been proven to be highly efficient in detecting Shigella in clinical samples compared to culture-based methods, which underestimate Shigella burden by 2- to 3-fold. qPCR assays have also been developed for Shigella speciation and serotyping, which is critical for both vaccine development and evaluation. METHODS: The Enterics for Global Health (EFGH) Shigella surveillance study will utilize a customized real-time PCR-based TaqMan Array Card (TAC) interrogating 82 targets, for the detection and differentiation of Shigella spp, Shigella sonnei , Shigella flexneri serotypes, other diarrhea-associated enteropathogens, and antimicrobial resistance (AMR) genes...
March 2024: Open Forum Infectious Diseases
https://read.qxmd.com/read/38532951/the-enterics-for-global-health-efgh-shigella-surveillance-study-in-peru
#5
JOURNAL ARTICLE
Katia Manzanares Villanueva, Tackeshy Pinedo Vasquez, Pablo Peñataro Yori, Lucero Romaina Cacique, Paul F Garcia Bardales, Wagner V Shapiama Lopez, Fiorella Zegarra Paredes, Karin F Perez, Silvia Rengifo Pinedo, Hermann Silva Delgado, Thomas Flynn, Francesca Schiaffino, Josh M Colston, Maribel Paredes Paredes Olortegui, Margaret N Kosek
BACKGROUND: The Enterics for Global Health (EFGH) Peru site will enroll subjects in a periurban area of the low Amazon rainforest. The political department of Loreto lags behind most of Peru in access to improved sources of water and sanitation, per capita income, children born <2.5 kg, and infant and child mortality. Chronic undernutrition as manifested by linear growth shortfalls is common, but wasting and acute malnutrition are not. METHODS: The recruitment of children seeking care for acute diarrheal disease takes place at a geographic cluster of government-based primary care centers in an area where most residents are beneficiaries of free primary healthcare...
March 2024: Open Forum Infectious Diseases
https://read.qxmd.com/read/38514309/-epidemiological-characteristics-of-diarrheagenic-escherichia-coli-infection-in-infectious-diarrhea-outpatients-aged-15-years-and-older-in-shanghai-2014-2021
#6
JOURNAL ARTICLE
S Lin, Y X Zheng, X H Gong, W J Xiao, X Yu, H Pan, J Chen
Objective: To understand the epidemiological characteristics of diarrheagenic Escherichia ( E. ) coli infection in infectious diarrhea outpatients aged 15 years and older in Shanghai and provide evidence for the development of disease control strategies . Methods: Based on multistage systematic sampling, diarrhea surveillance was conducted in 22 sentinel hospitals in Shanghai, the information about cases' demographic, clinical, and epidemiological characteristics were collected. Stool samples were collected for the detection and typing of diarrheagenic E...
March 10, 2024: Zhonghua Liu Xing Bing Xue za Zhi, Zhonghua Liuxingbingxue Zazhi
https://read.qxmd.com/read/38510585/detection-of-enteropathogens-in-human-immunodeficiency-virus-and-non-human-immunodeficiency-virus-infected-children-with-acute-diarrhea-in-an-indonesian-tertiary-hospital-using-multiplex-real-time-polymerase-chain-reaction
#7
JOURNAL ARTICLE
Dewi Wulandari, Rivaldi Febrian, Pramita Gayatri Dwipoerwantoro, Nia Kurniati
PURPOSE: Diarrhea is one of the leading causes of mortality in children living in developing countries. The etiology of acute diarrhea in each healthcare center varies depending on place, time, and population. This study aimed to identify pathogen patterns in human immunodeficiency virus (HIV)-infected and non-HIV children suffering from acute diarrhea, using multiplex real time reverse transcriptase polymerase chain reaction (RT-PCR), in an Indonesian tertiary hospital. METHODS: This cross-sectional study was conducted at Dr...
March 2024: Pediatric Gastroenterology, Hepatology & Nutrition
https://read.qxmd.com/read/38507962/colonization-of-the-gut-mucosa-of-colorectal-cancer-patients-by-pathogenic-mucosa-associated-escherichia-coli-strains
#8
JOURNAL ARTICLE
Roghayeh Nouri, Alka Hasani, Kourosh Masnadi Shirazi, Fatemeh Yeganeh Sefiadn, Fariba Naeimi Mazraeh, Simin Sattarpour, Mohammad Ahangarzadeh Rezaee
Some strains of Escherichia coli are known to be involved in the pathogenesis of colorectal cancer (CRC). The aim of current study was to compare the general characteristics of the E. coli from CRC patients and healthy participants. A total of 96 biopsy samples from 48 CRC patients and 48 healthy participants, were studied. The clonality of the E. coli isolates was analyzed by Enterobacterial repetitive intergenic consensus-based PCR (ERIC-PCR) method. The strains were tested by PCR to determine the prevalence of different virulence factors...
February 22, 2024: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/38497656/type-1-fimbriae-mediated-collective-protection-against-type-6-secretion-system-attacks
#9
JOURNAL ARTICLE
Margot Marie Dessartine, Artemis Kosta, Thierry Doan, Éric Cascales, Jean-Philippe Côté
Bacterial competition may rely on secretion systems such as the type 6 secretion system (T6SS), which punctures and releases toxic molecules into neighboring cells. To subsist, bacterial targets must counteract the threats posed by T6SS-positive competitors. In this study, we used a comprehensive genome-wide high-throughput screening approach to investigate the dynamics of interbacterial competition. Our primary goal was to identify deletion mutants within the well-characterized E. coli K-12 single-gene deletion library, the Keio collection, that demonstrated resistance to T6SS-mediated killing by the enteropathogenic bacterium Cronobacter malonaticus ...
March 18, 2024: MBio
https://read.qxmd.com/read/38485558/enterocolitis-and-other-immunotherapy-and-targeted-therapy-related-gastrointestinal-manifestations-a-review-for-gastroenterologist
#10
REVIEW
O Gómez Escudero
New oncologic treatments, particularly immunotherapy (IT), have revolutionized the treatment of advanced-stage malignant tumors. Immune checkpoint inhibitors are the main form of IT and act by increasing T cell activity and the organism's immune response against neoplastic cells. Targeted therapy is another form of IT that acts by inhibiting oncogenes or inflammation signaling and tumor angiogenesis pathways. However, these mechanisms of tumor destruction can interfere with the host's immune self-tolerance or with the mechanisms of epithelial tissue repair and predispose to immune system-mediated adverse events that can affect multiple organs, including the digestive tract...
March 13, 2024: Revista de Gastroenterología de México
https://read.qxmd.com/read/38479171/assessment-of-antibiotic-resistant-infection-risks-associated-with-reclaimed-wastewater-irrigation-in-intensive-tomato-cultivation
#11
JOURNAL ARTICLE
Samira Nahim-Granados, Hunter Quon, María Inmaculada Polo-López, Isabel Oller, Ana Agüera, Sunny Jiang
Agricultural irrigation using reclaimed urban wastewater (RWW) represents a sustainable practice to meet the ever-increasing water stress in modern societies. However, the occurrence of residual antibiotics and antibiotic resistant bacteria (ARB) in RWW is an important human health concern. This study applied for the first time a novel Simple-Death dose-response model to the field data of Escherichia coli and Pseudomonas spp. collected from three greenhouses for cultivation of tomatoes irrigated with RWW. The model estimates the risk of infection by enteropathogenic E...
March 7, 2024: Water Research
https://read.qxmd.com/read/38474124/o26-polysaccharides-as-key-players-in-enteropathogenic-e-coli-immune-evasion-and-vaccine-development
#12
JOURNAL ARTICLE
Thiago Jordão da Silva Lemos, Herbert Guimarães de Sousa Silva, José Osvaldo Previato, Lucia Mendonça-Previato, Elisangela Oliveira de Freitas, Angela Silva Barbosa, Marcia Regina Franzolin, Luis Fernando Dos Santos, Bruna de Sousa Melo, Geovana Ferreira Dos Anjos, Renata Hiromi Nakagima Gonçalves, Marta de Oliveira Domingos
Enteropathogenic Escherichia coli (EPEC) produce a capsule of polysaccharides identical to those composing the O-antigen polysaccharide of its LPS (lipopolysaccharide) molecules. In light of this, the impact of O26 polysaccharides on the immune evasion mechanisms of capsulated O26 EPEC compared to non-capsulated enterohemorrhagic Escherichia coli (EHEC) was investigated. Our findings reveal that there was no significant difference between the levels in EPEC and EHEC of rhamnose (2.8:2.5), a molecule considered to be a PAMP (Pathogen Associated Molecular Patterns)...
March 1, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38461791/enteric-coronavirus-pdcov-evokes-a-non-warburg-effect-by-hijacking-pyruvic-acid-as-a-metabolic-hub
#13
JOURNAL ARTICLE
Guanning Su, Jiao Liu, Chenrui Duan, Puxian Fang, Liurong Fang, Yanrong Zhou, Shaobo Xiao
The Warburg effect, also referred as aerobic glycolysis, is a common metabolic program during viral infection. Through targeted metabolomics combined with biochemical experiments and various cell models, we investigated the central carbon metabolism (CCM) profiles of cells infected with porcine deltacoronavirus (PDCoV), an emerging enteropathogenic coronavirus with zoonotic potential. We found that PDCoV infection required glycolysis but decreased glycolytic flux, exhibiting a non-Warburg effect characterized by pyruvic acid accumulation...
March 4, 2024: Redox Biology
https://read.qxmd.com/read/38457266/enteric-viruses-other-than-rotavirus-and-norovirus-in-children-under-5-years-of-age-with-gastroenteritis-in-argentina-2010-2021-a-descriptive-study
#14
JOURNAL ARTICLE
Juan I Degiuseppe, María T Soto, Christian Barrios Mathieur, Karina A Gomes, Juan A Stupka
Introduction. Data on the frequency of enteric adenoviruses, sapoviruses, and astroviruses in cases of sporadic acute gastroenteritis in Argentina are scarce. Methods. Descriptive design of a selection of fecal samples of children with diarrhea younger than 5 years referred between 2010 and 2021, with a previous negative result for rotavirus and norovirus. The presence of enteric adenovirus, sapovirus, and astrovirus was tested by molecular methods, with subsequent genotyping of positive samples. Results. At least 1 of the tested viruses was detected in 226 (39...
March 14, 2024: Archivos Argentinos de Pediatría
https://read.qxmd.com/read/38428867/epidemiology-of-pathogens-causing-acute-diarrhea-in-patients-presenting-to-the-emergency-departments-in-4-hospitals-in-lebanon
#15
JOURNAL ARTICLE
Mariana Helou, Janane Nasr, Micheline Hajjar, Alaa Bourji, Rita Feghaly, Elsy Jabbour, Edmond Abboud, Emma Abboud, Ahmad Mahdi, Jonathan Mina, Alain Asmar, Rola Husni
Acute diarrhea is a prevalent disease worldwide and a cause of mortality in low and middle-income countries. In previous studies, the causative enteropathogen is found in 38% to 58% of patients, leaving a considerable diagnostic gap. In this study, we intend to determine the pathogenic agents responsible for acute diarrhea in patients presenting to the Emergency Departments of several Lebanese hospitals. A total of 100 stool samples were collected between June 2022 and June 2023 from patients presenting with acute diarrhea to the Emergency Departments...
March 1, 2024: Medicine (Baltimore)
https://read.qxmd.com/read/38425667/norovirus-in-children-under-2-years-of-age-an-epidemiological-study-in-panama-during-the-covid-19-pandemic
#16
JOURNAL ARTICLE
Rodrigo DeAntonio, Morgan Hess-Holtz, Leyda Abrego, Zeuz Capitan-Barrios, Leyla Hernandez Donoso, Tirza De León, Xavier Sáez Llorens, Brechla Moreno, John Gerard Weil
INTRODUCTION: Norovirus infection is a common cause of acute gastroenteritis (AGE). Surveillance activities are important to aid investigation into effective norovirus control strategies, including vaccination. Here, we report ancillary findings related to the incidence, prevalence, and etiology of AGE caused by norovirus in Panama after adjustment of study methodology to comply with national coronavirus disease 2019 (COVID-19) mandates. METHODS: In January 2020, children aged <2 years began enrolling into an epidemiological study in Panama to estimate the burden of norovirus in preparation for evaluating upcoming prevention strategies...
2024: Frontiers in Pediatrics
https://read.qxmd.com/read/38417919/improving-gut-health-and-growth-in-early-life-a-protocol-for-an-individually-randomised-two-arm-open-label-controlled-trial-of-a-synbiotic-in-infants-in-kaffrine-district-senegal
#17
JOURNAL ARTICLE
Benjamin Momo Kadia, Marietou Khouma, Doudou Sow, Babacar Faye, Anouschka S Ramsteijn, Beatriz Calvo-Urbano, Modou L Jobarteh, Elaine Ferguson, Paul Haggarty, Joanne P Webster, Alan W Walker, Claire Heffernan, Stephen J Allen
INTRODUCTION: Infants exposed to enteropathogens through poor sanitation and hygiene can develop a subclinical disorder of the gut called environmental enteric dysfunction (EED), characterised by abnormal intestinal histology and permeability. EED can contribute to stunting through reduced digestion and absorption of nutrients, increased susceptibility to infections, increased systemic inflammation and inhibition of growth hormones. EED can be apparent by age 12 weeks, highlighting the need for early intervention...
February 27, 2024: BMJ Paediatrics Open
https://read.qxmd.com/read/38412156/the-planetary-child-health-enterics-observatory-plan-eo-a-protocol-for-an-interdisciplinary-research-initiative-and-web-based-dashboard-for-mapping-enteric-infectious-diseases-and-their-risk-factors-and-interventions-in-lmics
#18
JOURNAL ARTICLE
Josh M Colston, Bin Fang, Eric Houpt, Pavel Chernyavskiy, Samarth Swarup, Lauren M Gardner, Malena K Nong, Hamada S Badr, Benjamin F Zaitchik, Venkataraman Lakshmi, Margaret N Kosek
BACKGROUND: Diarrhea remains a leading cause of childhood illness throughout the world that is increasing due to climate change and is caused by various species of ecologically sensitive pathogens. The emerging Planetary Health movement emphasizes the interdependence of human health with natural systems, and much of its focus has been on infectious diseases and their interactions with environmental and human processes. Meanwhile, the era of big data has engendered a public appetite for interactive web-based dashboards for infectious diseases...
2024: PloS One
https://read.qxmd.com/read/38401379/whole-genome-sequencing-of-escherichia-coli-from-retail-meat-in-china-reveals-the-dissemination-of-clinically-important-antimicrobial-resistance-genes
#19
JOURNAL ARTICLE
Qin Wang, Ying-Yue Han, Tie-Jun Zhang, Xuan Chen, Heng Lin, Hong-Ning Wang, Chang-Wei Lei
Escherichia coli is one of the important reservoirs of antimicrobial resistance genes (ARG), which often causes food-borne diseases and clinical infections. Contamination with E. coli carrying clinically important antimicrobial resistance genes in retail meat products can be transmitted to humans through the food chain, posing a serious threat to public health. In this study, a total of 330 E. coli strains were isolated from 464 fresh meat samples from 17 food markets in China, two of which were identified as enterotoxigenic and enteropathogenic E...
February 22, 2024: International Journal of Food Microbiology
https://read.qxmd.com/read/38399648/detection-of-mcr-1-1-positive-enteropathogenic-escherichia-coli-isolates-associated-with-post-weaning-diarrhoea-in-an-organic-piglet-producing-farm-in-austria
#20
René Renzhammer, Lukas Schwarz, Adriana Cabal Rosel, Werner Ruppitsch, Andreas Fuchs, Erwin Simetzberger, Andrea Ladinig, Igor Loncaric
Postweaning diarrhoea (PWD) is a frequent multifactorial disease occurring in swine stocks worldwide. Since pathogenic Escherichia ( E .) coli play a pivotal role in the pathogenesis of PWD and porcine E. coli are often resistant to different antibiotics, colistin is frequently applied to treat piglets with PWD. However, the application of colistin to livestock has been associated with the emergence of colistin resistance. This case report describes the detection of the colistin resistance gene mcr-1-1 in two E...
January 24, 2024: Microorganisms
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