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https://www.readbyqxmd.com/read/28637341/assessment-of-workers-exposure-to-grain-dust-and-bioaerosols-during-the-loading-of-vessels-hold-an-example-at-a-port-in-the-province-of-qu%C3%A3-bec
#1
Geneviève Marchand, Marie Gardette, Kiet Nguyen, Valérie Amano, Eve Neesham-Grenon, Maximilien Debia
Longshoremen are exposed to large amounts of grain dust while loading of grain into the holds of vessels. Grain dust inhalation has been linked to respiratory diseases such as chronic bronchitis, hypersensitivity, pneumonitis, and toxic pneumonitis. Our objective was to characterize the exposure of longshoremen to inhalable and total dust, endotoxins, and cultivable bacteria and fungi during the loading of grain in a vessel's hold at the Port of Montreal in order to assess the potential health risks. Sampling campaigns were conducted during the loading of two different types of grain (wheat and corn)...
June 15, 2017: Annals of Work Exposures and Health
https://www.readbyqxmd.com/read/28637301/eden-evolutionary-dynamics-within-environments
#2
Philipp C Münch, Bärbel Stecher, Alice C McHardy
Summary: Metagenomics revolutionized the field of microbial ecology, giving access to Gb-sized datasets of microbial communities under natural conditions. This enables fine-grained analyses of the functions of community members, studies of their association with phenotypes and environments, as well as of their microevolution and adaptation to changing environmental conditions. However, phylogenetic methods for studying adaptation and evolutionary dynamics are not able to cope with big data...
June 16, 2017: Bioinformatics
https://www.readbyqxmd.com/read/28636915/probing-the-structural-dynamics-of-the-nmda-receptor-activation-by-coarse-grained-modeling
#3
Wenjun Zheng, Han Wen, Gary J Iacobucci, Gabriela K Popescu
N-Methyl-D-aspartate (NMDA) receptors are glutamate-gated excitatory channels that play essential roles in brain functions. High-resolution structures have been solved for an allosterically inhibited and agonist-bound form of a functional NMDA receptor; however, other key functional states (particularly the active open-channel state) were only resolved at moderate resolutions by cryo-electron microscopy (cryo-EM). To decrypt the mechanism of the NMDA receptor activation, structural modeling is essential to provide presently missing information about structural dynamics...
June 20, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28636825/a-new-mixed-all-atom-coarse-grained-model-application-to-melittin-aggregation-in-aqueous-solution
#4
Mee Y Shelley, Myvizhi Esai Selvan, Jun Zhao, Volodymyr Babin, Chenyi Liao, Jianing Li, John Clarence Shelley
We introduce a new mixed resolution, all-atom/coarse-grained approach (AACG) for modeling peptides in aqueous solution and apply it to characterizing the aggregation of melittin. All of the atoms in peptidic components are represented while a single site is used for each water molecule. With the full flexibility of the peptide retained, our AACG method achieves speedups by a factor of 3-4 for CPU time reduction and another factor of roughly 7 for diffusion. An Ewald treatment permits the inclusion of long-range electrostatic interactions...
June 21, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28636412/comparative-chemical-and-biological-studies-of-leaves-and-pollens-of-phoenix-canariensis-hort-ex-chabaud
#5
Mohamed S Hifnawy, Amr Mk Mahrous, Amany A Sleem, Rehab Ms Ashour
AIM:  Phoenix canariensis hort. ex Chabaud is one of the most important palm plants. In spite of that, it has not been extensively investigated from a phytochemical or a biological perspective. MATERIALS & METHODS: Air-dried powdered leaves and pollen grains of the plant were extracted using 70% ethanol. The residues were fractionated with different solvents of increasing polarity. Antioxidant, anti-inflammatory, hepatoprotective, anti-hyperglycemic activities were determined using adult male albino rats of wistar strain...
June 21, 2017: Future Medicinal Chemistry
https://www.readbyqxmd.com/read/28636095/suppression-or-knockout-of-saf-sam-overcomes-the-sa-mediated-hybrid-male-sterility-in-rice
#6
Yongyao Xie, Baixiao Niu, Yunming Long, Gousi Li, Jintao Tang, Yaling Zhang, Ding Ren, Yao-Guang Liu, Letian Chen
Hybrids between the indica and japonica subspecies of rice (Oryza sativa) are usually sterile, which hinders utilization of heterosis in the inter-subspecific hybrid breeding. The complex locus Sa comprises two adjacently located genes, SaF and SaM, which interact to cause abortion of pollen grains carrying the japonica allele in japonica-indica hybrids. Here we showed that silencing of SaF or SaM by RNA interference restored male fertility in indica-japonica hybrids with heterozygous Sa. We further used CRISPR/Cas9-based genome editing to knockout the SaF and SaM alleles respectively of an indica rice line to create hybrid-compatible lines...
June 21, 2017: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/28635680/saturated-fatty-acids-and-cardiovascular-disease-replacements-for-saturated-fat-to-reduce-cardiovascular-risk
#7
REVIEW
Michelle A Briggs, Kristina S Petersen, Penny M Kris-Etherton
Dietary recommendations to decrease the risk of cardiovascular disease (CVD) have focused on reducing intake of saturated fatty acids (SFA) for more than 50 years. While the 2015-2020 Dietary Guidelines for Americans advise substituting both monounsaturated and polyunsaturated fatty acids for SFA, evidence supports other nutrient substitutions that will also reduce CVD risk. For example, replacing SFA with whole grains, but not refined carbohydrates, reduces CVD risk. Replacing SFA with protein, especially plant protein, may also reduce CVD risk...
June 21, 2017: Healthcare (Basel, Switzerland)
https://www.readbyqxmd.com/read/28635630/development-of-a-high-density-snp-based-linkage-map-and-detection-of-qtl-for-%C3%AE-glucans-protein-content-grain-yield-per-spike-and-heading-time-in-durum-wheat
#8
Ilaria Marcotuli, Agata Gadaleta, Giacomo Mangini, Antonio Massimo Signorile, Silvana Addolorata Zacheo, Antonio Blanco, Rosanna Simeone, Pasqualina Colasuonno
High-density genetic linkage maps of crop species are particularly useful in detecting qualitative and quantitative trait loci for important agronomic traits and in improving the power of classical approaches to identify candidate genes. The aim of this study was to develop a high-density genetic linkage map in a durum wheat recombinant inbred lines population (RIL) derived from two elite wheat cultivars and to identify, characterize and correlate Quantitative Trait Loci (QTL) for β-glucan, protein content, grain yield per spike and heading time...
June 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28635622/the-arabidopsis-gpr1-gene-negatively-affects-pollen-germination-pollen-tube-growth-and-gametophyte-senescence
#9
Xiao Yang, Qinying Zhang, Kun Zhao, Qiong Luo, Shuguang Bao, Huabin Liu, Shuzhen Men
Genes essential for gametophyte development and fertilization have been identified and studied in detail; however, genes that fine-tune these processes are largely unknown. Here, we characterized an unknown Arabidopsis gene, GTP-BINDING PROTEIN RELATED1 (GPR1). GPR1 is specifically expressed in ovule, pollen, and pollen tube. Enhanced green fluorescent protein-tagged GPR1 localizes to both nucleus and cytoplasm, and it also presents in punctate and ring-like structures. gpr1 mutants exhibit no defect in gametogenesis and seed setting, except that their pollen grains are pale in color...
June 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28635295/coarse-grained-models-of-aqueous-and-pure-liquid-alkanes
#10
Gaurav Gyawali, Samuel Sternfield, Revati Kumar, Steven W Rick
A model for linear alkanes is presented in which interaction sites are only on the carbon atoms and the range of the potential is reduced using the Stillinger-Weber potential. The model is optimized for aqueous and liquid alkane properties and can match thermodynamic and structural properties, including solvation free energies, liquid densities, and liquid/vapor and liquid/water surface tensions for alkanes over a range of lengths.The results for long alkanes indicates that such models can be useful as accurate, yet efficient, coarse-grained potentials for macromolecules in water and other environments...
June 21, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28635264/cadmium-accumulation-risk-in-vegetables-and-rice-in-southern-china-insights-from-solid-solution-partitioning-and-plant-uptake-factor
#11
Yang Yang, Meie Wang, Weiping Chen, Yanling Li, Chi Peng
Solid-solution partitioning coefficient (Kd) and plant uptake factor (PUF) largely determine the solubility and mobility of soil Cd to food crops. A four-year regional investigation was conducted in contaminated vegetable and paddy fields of southern China to quantify the variability in Kd and PUF. The distributions of Kd and PUF characterizing transfers of Cd from soil to vegetable and rice are probabilistic in nature. Dynamics in soil pH and soil Zn greatly affected the variations of Kd. In addition to soil pH, soil organic matter had a major influence on PUF variations in vegetables...
June 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28635174/flash-light-millisecond-self-assembly-of-high-%C3%AF-block-copolymers-for-wafer-scale-sub-10-nm-nanopatterning
#12
Hyeong Min Jin, Dae Yong Park, Seong-Jun Jeong, Gil Yong Lee, Ju Young Kim, Jeong Ho Mun, Seung Keun Cha, Joonwon Lim, Jun Soo Kim, Kwang Ho Kim, Keon Jae Lee, Sang Ouk Kim
One of the fundamental challenges encountered in successful incorporation of directed self-assembly in sub-10 nm scale practical nanolithography is the process compatibility of block copolymers with a high Flory-Huggins interaction parameter (χ). Herein, reliable, fab-compatible, and ultrafast directed self-assembly of high-χ block copolymers is achieved with intense flash light. The instantaneous heating/quenching process over an extremely high temperature (over 600 °C) by flash light irradiation enables large grain growth of sub-10 nm scale self-assembled nanopatterns without thermal degradation or dewetting in a millisecond time scale...
June 21, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28634800/impact-of-chemical-leaching-on-permeability-and-cadmium-removal-from-fine-grained-soils
#13
Zhongbing Lin, Renduo Zhang, Shuang Huang, Kang Wang
The aim of this study was to investigate the influence of chemical leaching on permeability and Cd removal from fine-grained polluted soils. Column leaching experiments were conducted using two types of soils (i.e., artificially Cd-polluted loam and historically polluted silty loam). Chemical agents of CaCl2, FeCl3, citric acid, EDTA, rhamnolipid, and deionized water were used to leach Cd from the soils. Results showed that organic agents reduced permeability of both soils, and FeCl3 reduced permeability of loam soil, compared with inorganic agents and deionized water...
June 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28634797/distribution-transfer-and-health-risks-of-polycyclic-aromatic-hydrocarbons-pahs-in-soil-wheat-systems-of-henan-province-a-typical-agriculture-province-of-china
#14
Jinglan Feng, Xiaoying Li, Jiahui Zhao, Jianhui Sun
In order to investigate the distribution, transfer, and human health risks of polycyclic aromatic hydrocarbons (PAHs) in the soil-wheat systems, soil samples from 20 farmlands and corresponding wheat tissues were collected from selected regions of Henan Province in June 2013 and were analyzed to estimate the concentration of PAHs. The total concentrations of 15 PAHs (∑15 PAHs) in soils from Henan Province varied from 6.91 to 72.4 ng/g. Moreover, two-ring to three-ring PAHs (1.59-29.1 ng/g) were the major species in soils, occupying 56...
June 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28634483/elicitor-induced-biochemical-and-molecular-manifestations-to-improve-drought-tolerance-in-rice-oryza-sativa-l-through-seed-priming
#15
Mahesh K Samota, Minnu Sasi, Monika Awana, Om P Yadav, S V Amitha Mithra, Aruna Tyagi, Suresh Kumar, Archana Singh
Rice (Oryza sativa L.) is one of the major grain cereals of the Indian subcontinent which face water-deficit stress for their cultivation. Seed-priming has been reported to be a useful approach to complement stress responses in plants. In the present study, seed-priming with hormonal or chemical elicitor [viz. methyl jasmonate (MJ), salicylic acid (SA), paclobutrazol (PB)] showed significant increase in total phenolic content, antioxidant activity and expression of Rice Drought-responsive (RD1 and RD2) genes (of AP2/ERF family) in contrasting rice genotypes (Nagina-22, drought-tolerant and Pusa Sugandh-5, drought-sensitive) under drought stress...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28634333/atomic-rearrangement-of-a-sputtered-mos2-film-from-amorphous-to-a-2d-layered-structure-by-electron-beam-irradiation
#16
Bong Ho Kim, Hyun Ho Gu, Young Joon Yoon
We synthesised a crystalline MoS2 film from as-sputtered amorphous film by applying an electron beam irradiation (EBI) process. A collimated electron beam (60 mm dia.) with an energy of 1 kV was irradiated for only 1 min to achieve crystallisation without an additional heating process. After the EBI process, we observed a two-dimensional layered structure of MoS2 about 4 nm thick and with a hexagonal atomic arrangement on the surface. A stoichiometric MoS2 film was confirmed to grow well on SiO2/Si substrates and include partial oxidation of Mo...
June 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28634304/redox-dependent-interaction-between-thaumatin-like-protein-and-%C3%AE-glucan-influences-malting-quality-of-barley
#17
Surinder Singh, Rajiv K Tripathi, Peggy G Lemaux, Bob B Buchanan, Jaswinder Singh
Barley is the cornerstone of the malting and brewing industry. It is known that 250 quantitative trait loci (QTLs) of the grain are associated with 19 malting-quality phenotypes. However, only a few of the contributing genetic components have been identified. One of these, on chromosome 4H, contains a major malting QTL, QTL2, located near the telomeric region that accounts, respectively, for 28.9% and 37.6% of the variation in the β-glucan and extract fractions of malt. In the current study, we dissected the QTL2 region using an expression- and microsynteny-based approach...
June 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28634293/intrinsic-map-dynamics-exploration-for-uncharted-effective-free-energy-landscapes
#18
Eliodoro Chiavazzo, Roberto Covino, Ronald R Coifman, C William Gear, Anastasia S Georgiou, Gerhard Hummer, Ioannis G Kevrekidis
We describe and implement a computer-assisted approach for accelerating the exploration of uncharted effective free-energy surfaces (FESs). More generally, the aim is the extraction of coarse-grained, macroscopic information from stochastic or atomistic simulations, such as molecular dynamics (MD). The approach functionally links the MD simulator with nonlinear manifold learning techniques. The added value comes from biasing the simulator toward unexplored phase-space regions by exploiting the smoothness of the gradually revealed intrinsic low-dimensional geometry of the FES...
June 20, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28633811/effects-and-mechanism-of-ultrasonic-irradiation-on-solidification-microstructure-and-mechanical-properties-of-binary-tial-alloys
#19
Ruirun Chen, Deshuang Zheng, Tengfei Ma, Hongsheng Ding, Yanqing Su, Jingjie Guo, Hengzhi Fu
In spite of their high temperature and reactivity, the binary TiAl alloys are successfully imposed by the ultrasonic irradiation and the microstructure evolution, solidification behaviors and mechanical properties are elaborately investigated. After ultrasonic irradiation, a high quality ingot without shrinkage defects and element segregation is obtained and the coarse dendrite structure is well modified into fine non-dendrite globular grains. The coarse lamellar colony and lamellar space of Ti44Al alloy is refined from 685μm to 52μm and 1185nm to 312nm, respectively (similarly, 819μm to 102μm and 2085nm to 565nm for Ti48Al alloy)...
September 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28633759/martini-coarse-grained-force-field-extension-to-rna
#20
Jaakko J Uusitalo, Helgi I Ingólfsson, Siewert J Marrink, Ignacio Faustino
RNA has an important role not only as the messenger of genetic information but also as a regulator of gene expression. Given its central role in cell biology, there is significant interest in studying the structural and dynamic behavior of RNA in relation to other biomolecules. Coarse-grain molecular dynamics simulations are a key tool to that end. Here, we have extended the coarse-grain Martini force field to include RNA after our recent extension to DNA. In the same way DNA was modeled, the tertiary structure of RNA is constrained using an elastic network...
June 17, 2017: Biophysical Journal
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