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https://www.readbyqxmd.com/read/29671758/anthocyanin-composition-and-content-in-rye-plants-with-different-grain-color
#1
Pavel A Zykin, Elena A Andreeva, Anna N Lykholay, Natalia V Tsvetkova, Anatoly V Voylokov
The color of grain in cereals is determined mainly by anthocyanin pigments. A large level of genetic diversity for anthocyanin content and composition in the grain of different species was observed. In rye, recessive mutations in six genes (vi1...vi6) lead to the absence of anthocyanins in all parts of the plant. Moreover, dominant genes of anthocyanin synthesis in aleurone (gene C) and pericarp (gene Vs) also affect the color of the grain. Reverse phase high-performance liquid chromatography and mass spectrometry were used to study anthocyanins in 24 rye samples...
April 19, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29660003/temperature-and-nitrogen-supply-interact-to-determine-protein-distribution-gradients-in-the-wheat-grain-endosperm
#2
George Savill, Adam Michalski, Stephen J Powers, Yongfang Wan, Paola Tosi, Peter Buchner, Malcolm J Hawkesford
Gradients exist in the distribution of storage proteins in the wheat (Triticum aestivum L.) endosperm and determine the milling properties and protein recovery rate of the grain. A novel image analysis technique was developed to quantify both the gradients in protein concentration, and the size distribution of protein bodies within the endosperm of wheat plants grown under two different (20 °C or 28 °C) post-anthesis temperatures, and supplied with a nutrient solution with either high or low nitrogen content...
April 5, 2018: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29658925/measuring-gene-expression-in-bombarded-barley-aleurone-layers-with-increased-throughput
#3
Grace Uwase, Taylor P Enrico, David S Chelimo, Benjamin R Keyser, Russell R Johnson
The aleurone layer of barley grains is an important model system for hormone-regulated gene expression in plants. In aleurone cells, genes required for germination or early seedling development are activated by gibberellin (GA), while genes associated with stress responses are activated by abscisic acid (ABA). The mechanisms of GA and ABA signaling can be interrogated by introducing reporter gene constructs into aleurone cells via particle bombardment, with the resulting transient expression measured using enzyme assays...
March 30, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29610577/profiling-and-quantification-of-grain-anthocyanins-in-purple-pericarp-%C3%A3-blue-aleurone-wheat-crosses-by-high-performance-thin-layer-chromatography-and-densitometry
#4
Stefan Böhmdorfer, Josua Timotheus Oberlerchner, Christina Fuchs, Thomas Rosenau, Heinrich Grausgruber
Background: Anthocyanins are abundant secondary metabolites responsible for most blue to blue-black, and red to purple colors of various plant organs. In wheat grains, anthocyanins are accumulated in the pericarp and/or aleurone layer. Anthocyanin pigmented wheat grains can be processed into functional foods with potential health benefits due to the antioxidant properties of the anthocyanins. The grain anthocyanin content can be increased by pyramidizing the different genes responsible for the accumulation of anthocyanins in the different grain layers...
2018: Plant Methods
https://www.readbyqxmd.com/read/29593765/zinc-and-iron-concentration-as-affected-by-nitrogen-fertilization-and-their-localization-in-wheat-grain
#5
Bal R Singh, Yadu N Timsina, Ole C Lind, Simone Cagno, Koen Janssens
Nearly half of the world cereal production comes from soils low or marginal in plant available zinc, leading to unsustainable and poor quality grain production. Therefore, the effects of nitrogen (N) rate and application time on zinc (Zn) and iron (Fe) concentration in wheat grain were investigated. Wheat ( Triticum aestivum var. Krabat) was grown in a growth chamber with 8 and 16 h of day and night periods, respectively. The N rates were 29, 43, and 57 mg N kg-1 soil, equivalent to 80, 120, and 160 kg N ha-1 ...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29569870/novel-insights-into-pericarp-protein-body-globoids-of-aleurone-layer-starchy-granules-of-three-cereals-gained-using-atomic-force-microscopy-and-environmental-scanning-electronic-microscopy
#6
Elena Antonini, Carolina Zara, Laura Valentini, Pietro Gobbi, Paolino Ninfali, Michele Menotta
In this study, we applied Environmental Scanning Electron Microscopy-Energy Dispersive Spectroscopy (ESEM-EDS) and Atomic Force Microscopy (AFM) analysis to three different cereal caryopses: barley, oat and einkorn wheat. The morphological structures, chemical elemental composition and surface characteristics of the three cereals were described. Regarding the morphology, barley showed the thickest pericarp, providing a strong barrier digestion and absorption of nutrients. The aleurone layer of each cereal type contained protein body globoids within its cells...
February 5, 2018: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/29547863/effects-of-exogenous-gamma-aminobutyric-acid-on-%C3%AE-amylase-activity-in-the-aleurone-of-barley-seeds
#7
Yidi Sheng, Huiyuan Xiao, Chunli Guo, Hong Wu, Xiaojing Wang
Gamma-aminobutyric acid (GABA), a nonprotein amino acid, often accumulates in plants exposed to certain environmental stimuli. Previous studies indicated that a closed relationship existed between endogenous GABA and seed germination. However, there are few studies on the effect of exogenous GABA on seed germination. The objective of this study was to explore whether exogenous GABA affected α-amylase activity which the activation is an important stage in seed germination. The level of endogenous GABA in barley seeds rose gradually during germination, suggesting that endogenous GABA was involved in germination...
March 3, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29542137/characterizing-bread-wheat-genotypes-of-pakistani-origin-for-grain-zinc-biofortification-potential
#8
Abdul Rehman, Muhammad Farooq, Ahmad Nawaz, Abdullah M Al-Sadi, Khalid Al-Hashmi, Faisal Nadeem, Aman Ullah
BACKGROUND: Zinc (Zn) is essential for all life forms and its deficiency represents the major issue of malnutrition in humans. This study was carried out to characterize the 28 wheat genotypes of Pakistani origin for grain zinc biofortification potential, genetic diversity and relatedness. RESULTS: There was low genetic differentiation among the tested genotypes. However, these wheat genotypes differed greatly for yield related traits and grain mineral concentration (Zn, Ca and protein) and Zn bioavailability...
March 15, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29449858/exogenous-hydrogen-peroxide-contributes-to-heme-oxygenase-1-delaying-programmed-cell-death-in-isolated-aleurone-layers-of-rice-subjected-to-drought-stress-in-a-cgmp-dependent-manner
#9
Guanghui Wang, Yu Xiao, Xiaojiang Deng, Heting Zhang, Tingge Li, Huiping Chen
Hydrogen peroxide (H 2 O 2 ) is a reactive oxygen species (ROS) that plays a dual role in plant cells. Here, we discovered that drought (20% polyethylene glycol-6000, PEG)-triggered decreases of HO-1 transcript expression and HO activity. However, exogenous H 2 O 2 contributed toward the increase in HO-1 gene expression and activity of the enzyme under drought stress. Meanwhile, the HO-1 inducer hematin could mimic the effects of the H 2 O 2 scavengers ascorbic acid (AsA) and dimethylthiourea (DMTU) and the H 2 O 2 synthesis inhibitor diphenyleneiodonium (DPI) for scavenging or diminishing drought-induced endogenous H 2 O 2 ...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29416529/prebiotic-wheat-bran-fractions-induce-specific-microbiota-changes
#10
Kevin D'hoe, Lorenza Conterno, Francesca Fava, Gwen Falony, Sara Vieira-Silva, Joan Vermeiren, Kieran Tuohy, Jeroen Raes
Wheat bran fibers are considered beneficial to human health through their impact on gut microbiota composition and activity. Here, we assessed the prebiotic potential of selected bran fractions by performing a series of fecal slurry anaerobic fermentation experiments using aleurone as well as total, ultrafine, and soluble wheat bran (swb) as carbon sources. By combining amplicon-based community profiling with a fluorescent in situ hybridization (FISH) approach, we found that incubation conditions favor the growth of Proteobacteria such as Escherichia and Bilophila...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29397987/fingerprinting-and-characterization-of-anthocyanins-in-94-colored-wheat-varieties-and-blue-aleurone-and-purple-pericarp-wheat-crosses
#11
Stephanie Krüger, Gertrud E Morlock
Colored wheat varieties and crosses were analyzed to figure out their anthocyanin profiles, and thus, their potential as health-related food. After method development, the obtained 94 anthocyanin fingerprints allowed the clear differentiation of the blue aleurone and purple pericarp genotypes as well as their breeding lines. The method was trimmed so that the complete analysis of the whole grain flour including sample preparation of up to 20 samples on one plate took less than 3 h (<9 min per sample) and total costs including sample preparation were <1...
February 2, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29393510/sweet11-and-15-as-key-players-in-seed-filling-in-rice
#12
Jungil Yang, Dangping Luo, Bing Yang, Wolf B Frommer, Joon-Seob Eom
Despite the relevance of seed-filling mechanisms for crop yield, we still have only a rudimentary understanding of the transport processes that supply the caryopsis with sugars. We hypothesized that SWEET sucrose transporters may play important roles in nutrient import pathways in the rice caryopsis. We used a combination of mRNA quantification, histochemical analyses, translational promoter-reporter fusions and analysis of knockout mutants created by genomic editing to evaluate the contribution of SWEET transporters to seed filling...
February 2, 2018: New Phytologist
https://www.readbyqxmd.com/read/29387363/in-situ-production-of-active-vitamin-b12-in-cereal-matrices-using-propionibacterium-freudenreichii
#13
Bhawani Chamlagain, Tessa A Sugito, Paulina Deptula, Minnamari Edelmann, Susanna Kariluoto, Pekka Varmanen, Vieno Piironen
The in situ production of active vitamin B12 was investigated in aqueous cereal-based matrices with three strains of food-grade Propionibacterium freudenreichii. Matrices prepared from malted barley flour (33% w/v; BM), barley flour (6%; BF), and wheat aleurone (15%; AM) were fermented. The effect of cobalt and the lower ligand 5,6-dimethylbenzimidazole (DMBI) or its natural precursors (riboflavin and nicotinamide) on active B12 production was evaluated. Active B12 production was confirmed by UHPLC-UV-MS analysis...
January 2018: Food Science & Nutrition
https://www.readbyqxmd.com/read/29342075/enrichment-and-identification-of-the-most-abundant-zinc-binding-proteins-in-developing-barley-grains-by-zinc-imac-capture-and-nano-lc-ms-ms
#14
Giuseppe Dionisio, Mohammad Nasir Uddin, Eva Vincze
Background: Zinc accumulates in the embryo, aleurone, and subaleurone layers at different amounts in cereal grains. Our hypothesis is that zinc could be stored bound, not only to low MW metabolites/proteins, but also to high MW proteins as well. Methods: In order to identify the most abundant zinc binding proteins in different grain tissues, we microdissected barley grains into (1) seed coats; (2) aleurone/subaleurone; (3) embryo; and (4) endosperm. Initial screening for putative zinc binding proteins from the different tissue types was performed by fractionating proteins according to solubility (Osborne fractionation), and resolving those via Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) followed by polyvinylidene fluoride (PVDF) membrane blotting and dithizone staining...
January 17, 2018: Proteomes
https://www.readbyqxmd.com/read/29247595/method-for-hull-less-barley-transformation-and-manipulation-of-grain-mixed-linkage-beta-glucan
#15
Wai Li Lim, Helen M Collins, Rohan R Singh, Natalie A J Kibble, Kuok Yap, Jillian Taylor, Geoffrey B Fincher, Rachel A Burton
Hull-less barley is increasingly offering scope for breeding grains with improved characteristics for human nutrition; however, recalcitrance of hull-less cultivars to transformation has limited the use of these varieties. To overcome this limitation, we sought to develop an effective transformation system for hull-less barley using the cultivar Torrens. Torrens yielded a transformation efficiency of 1.8%, using a modified Agrobacterium transformation method. This method was used to over-express genes encoding synthases for the important dietary fiber component, (1,3;1,4)-β-glucan (mixed-linkage glucan), primarily present in starchy endosperm cell walls...
December 16, 2017: Journal of Integrative Plant Biology
https://www.readbyqxmd.com/read/29164348/delineation-of-inheritance-pattern-of-aleurone-layer-colour-through-chemical-tests-in-rice
#16
Chandu Singh, Sripathy K V, Jeevan Kumar S P, Bhojaraja Naik K, Govind Pal, Udaya Bhaskar K, Ramesh K V, Somasundaram G
BACKGROUND: Rice aleurone layer develops different colours with various chemical tests that may help to develop some rapid tests for identification/grouping of rice varieties. Understanding the colour inheritance pattern could enable to develop chemical clues that may help for genetic purity analysis along with grow-out-test. RESULTS: In this study, inheritance pattern of aleurone layer colour was studied in parents, F1 and F2 progenies derived from the crosses IR 36 × Acc...
November 21, 2017: Rice
https://www.readbyqxmd.com/read/29155872/sulfur-dioxide-alleviates-programmed-cell-death-in-barley-aleurone-by-acting-as-an-antioxidant
#17
Sha-Sha Wang, Ying-Xin Zhang, Feng Yang, Zhong-Qin Huang, Jun Tang, Kang-Di Hu, Hua Zhang
Sulfur dioxide (SO2), a gaseous signaling molecule in animal cells, has recently been found to play a physiological role in plants. Here we studied the role of SO2 in gibberellic acid (GA3)-induced programmed cell death (PCD) in barley (Hordeum vulgare L.) aleurone layers. The application of the SO2 donor (NaHSO3/Na2SO3, 1:3 M/M) effectively alleviated PCD in barley aleurone layers in a dose-dependent manner with an optimal concentration of 50 μM. Further investigations showed that SO2 reduced the accumulation of hydrogen peroxide (H2O2), superoxide anion (⋅O2-) and malondialdehyde (MDA) in aleurone layers...
2017: PloS One
https://www.readbyqxmd.com/read/29143621/identification-and-characterization-of-regulatory-network-components-for-anthocyanin-synthesis-in-barley-aleurone
#18
Ksenia V Strygina, Andreas Börner, Elena K Khlestkina
BACKGROUND: Among natural populations, there are different colours of barley (Hordeum vulgare L.). The colour of barley grains is directly related to the accumulation of different pigments in the aleurone layer, pericarp and lemma. Blue grain colour is due to the accumulation of anthocyanins in the aleurone layer, which is dependent on the presence of five Blx genes that are not sequenced yet (Blx1, Blx3 and Blx4 genes clustering on chromosome 4HL and Blx2 and Blx5 on 7HL). Due to the health benefits of anthocyanins, blue-grained barley can be considered as a source of dietary food...
November 14, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29091444/using-synchrotron-based-approaches-to-examine-the-foliar-application-of-znso4-and-zno-nanoparticles-for-field-grown-winter-wheat
#19
Teng Zhang, Hongda Sun, Zhiyuan Lv, Lili Cui, Hui Mao, Peter M Kopittke
The effects of foliar-applied ZnO nanoparticles (NPs) and ZnSO4 on winter wheat (Triticum aestivum L.) grain yield and grain quality were studied under field conditions, with the distribution and speciation of Zn within the grain examined using synchrotron-based X-ray fluorescence microscopy and X-ray absorption spectroscopy. Although neither of the two Zn compounds improved grain yield or quality, both increased the grain Zn concentration (average increments were 5 and 10 mg/kg for ZnSO4 and ZnO NPs treatments, respectively)...
November 1, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28982156/postprandial-pyy-increase-by-resistant-starch-supplementation-is-independent-of-net-portal-appearance-of-short-chain-fatty-acids-in-pigs
#20
Anne Krog Ingerslev, Shivaprakash Jagalur Mutt, Helle Nygaard Lærke, Mette Skou Hedemann, Peter Kappel Theil, Kirstine Lykke Nielsen, Henry Jørgensen, Karl-Heinz Herzig, Knud Erik Bach Knudsen
Increased dietary fiber (DF) fermentation and short-chain fatty acid (SCFA) production may stimulate peptide tyrosine-tyrosine (PYY) secretion. In this study, the effects of hindgut SCFA production on postprandial PYY plasma levels were assessed using different experimental diets in a porto-arterial catheterized pig model. The pigs were fed experimental diets varying in source and levels of DF for one week in 3×3 Latin square designs. The DF sources were whole-wheat grain, wheat aleurone, rye aleurone-rich flour, rye flakes, and resistant starch...
2017: PloS One
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