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https://www.readbyqxmd.com/read/28337309/gkn2-increases-apoptosis-reduces-the-proliferation-and-invasion-ability-of-gastric-cancer-cells-through-down-regulating-the-jak-stat-signaling-pathway
#1
Jun Ouyang, Xiaohui Pan, Hui Lin, Zecheng Hu, Ping Xiao, Haobin Hu
OBJECTIVES: To investigate the effect of gastric motility protein 2 (GKN2) on the proliferation, apoptosis and invasion of gastric cancer cell and on the JAK/signal transducer and activator of transcription 3 (STAT3) signaling pathway. METHODS: Expression of GKN2 was qualified using Western blot analysis in four gastric cancer cell lines and immortalized human gastric mucosal epithelial cell line GES-1. The cells were then transfected with pcDNA3.1-GKN2 and control vector using Lipofectamine(tm)2000 and assayed for viability, apoptosis, cell cycle changes, invasion ability as well as expression of cell cycle protein D1 (Cylin D1), Bcl-2, Bax, matrix metalloproteinase 2 (MMP2), MMP9, JAK2 and p-STAT3...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28337277/effect-and-mechanism-of-shed-on-ulcer-wound-healing-in-sprague-dawley-rat-models-with-diabetic-ulcer
#2
Yue Lv, Lihong Ge, Yuming Zhao
To evaluate the effect of stem cells from human exfoliated deciduous teeth (SHED) upon the ulcer wound healing and evaluate the mechanism underlying the role of SHED in Sprague-Dawley rat models with diabetic foot ulcer. The rats with diabetic ulcer were established and treated with SHED, mesenchymal stem cell (MSC) and PBS, respectively. The expression of vascular endothelial growth factor (VEGF), endothelial nitric oxide synthase (eNOS), matrix metalloproteinase-2 (MMP-2) and MMP9 at both protein and RNA levels was quantitatively measured...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28335383/a-biomedical-investigation-of-the-hepatoprotective-effect-of-radix-salviae-miltiorrhizae-and-network-pharmacology-based-prediction-of-the-active-compounds-and-molecular-targets
#3
Ming Hong, Sha Li, Ning Wang, Hor-Yue Tan, Fan Cheung, Yibin Feng
Radix salviae miltiorrhizae (Danshen in Chinese), a classic traditional Chinese medicine (TCM) herb, has been used for centuries to treat liver diseases. In this study, the preventive and curative potential of Danshen aqueous extract on acute/chronic alcoholic liver disease (ALD) and non-alcoholic fatty liver disease (NAFLD) was studied. The in vivo results indicated that Danshen could alleviate hepatic inflammation, fatty degeneration, and haptic fibrogenesis in ALD and NAFLD models. In the aspect of mechanism of action, the significant reduction in MDA levels in both ALD and NAFLD models implies the decreased levels of oxidative stress by Danshen...
March 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28335083/down-regulation-of-dab2ip-promotes-colorectal-cancer-invasion-and-metastasis-by-translocating-hnrnpk-into-nucleus-to-enhance-the-transcription-of-mmp2
#4
X H Zhu, J M Wang, S S Yang, F F Wang, J L Hu, S N Xin, H Men, G F Lu, X L Lan, D Zhang, X Y Wang, W T Liao, Y Q Ding, L Liang
DOC-2/DAB2 interacting protein (DAB2IP) is a RasGAP protein that shows a suppressive effect on cancer progression. Our previous study showed the involvement of transcription regulation of DAB2IP in metastasis of colorectal cancer (CRC). However, the molecular mechanisms of DAB2IP in regulating the progression of CRC need to be further explored. Here, we identified heterogeneous nuclear ribonucleoprotein K (hnRNPK) and matrix metalloproteinase 2 (MMP2) as vital downstream targets of DAB2IP in CRC cells by two-dimensional fluorescence difference gel electrophoresis and cDNA microassay, respectively...
March 23, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28329136/chronic-resveratrol-treatment-inhibits-mrc5-fibroblast-sasp-related-protumoral-effects-on-melanoma-cells
#5
Beatrice Menicacci, Anna Laurenzana, Anastasia Chillà, Francesca Margheri, Silvia Peppicelli, Elisabetta Tanganelli, Gabriella Fibbi, Lisa Giovannelli, Mario Del Rosso, Alessandra Mocali
Cellular senescence is related to organismal aging and is observed after DNA damaging cancer therapies, that induce tumor-suppressive modifications, but it is characterized by a strong increase in secreted factors, termed the "senescence-associated secretory phenotype" (SASP). Particularly, SASP from stroma senescent fibroblasts creates a cancer-favoring microenvironment, providing targets for anti-cancer interventions. In the present article, chronic treatment (5 weeks) with 5 µM resveratrol has been used to modulate senescence-related protumoral features of MRC5 fibroblasts, reducing SASP-related interleukins IL1α, IL1β, IL6, and IL8; transforming-growth-factor-β (TGFβ); matrix metallo-proteinases MMP3 and MMP2; urokinase plasminogen activator (uPA); receptor proteins uPAR, IL6R, insulin growth factor receptor-1 (IGF-1R), TGFβ-R2, and CXCR4...
January 20, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/28325826/a-phase-ii-randomized-double-blind-presurgical-trial-of-polyphenon-e-in-bladder-cancer-patients-to-evaluate-pharmacodynamics-and-bladder-tissue-biomarkers
#6
Jason R Gee, Daniel R Saltzstein, KyungMann Kim, Jill M Kolesar, Wei Huang, Thomas C Havighurst, Barbara W Wollmer, Jeanne Stublaski, Tracy Downs, Hasan Mukhtar, Margaret House, Howard L Parnes, Howard H Bailey
We performed a phase 2 pharmacodynamic, prevention trial of Polyphenon E® (a green tea polyphenol formulation primarily consisting of epigallocatechin gallate (EGCG)) in patients prior to bladder cancer surgery. Patients with a bladder tumor were randomized to receive Polyphenon E® containing either 800 or 1200 mg of EGCG or placebo for 14 to 28 days prior to TURBT or cystectomy. The primary objective was to compare the post-intervention EGCG tissue levels in patients receiving Polyphenon E® as compared to placebo...
March 21, 2017: Cancer Prevention Research
https://www.readbyqxmd.com/read/28323005/macrophage-migration-inhibitory-factor-interacts-with-thioredoxin-interacting-protein-and-induces-nf-%C3%AE%C2%BAb-activity
#7
Mi Jeong Kim, Won Sam Kim, Dong Oh Kim, Jae-Eun Byun, Hangsak Huy, Soo Yun Lee, Hae Young Song, Young-Jun Park, Tae-Don Kim, Suk Ran Yoon, Eun-Ji Choi, Hyunjung Ha, Haiyoung Jung, Inpyo Choi
The nuclear factor kappa B (NF-κB) pathway is pivotal in controlling survival and apoptosis of cancer cells. Macrophage migration inhibitory factor (MIF), a cytokine that regulates the immune response and tumorigenesis under inflammatory conditions, is upregulated in various tumors. However, the intracellular functions of MIF are unclear. In this study, we found that MIF directly interacted with thioredoxin-interacting protein (TXNIP), a tumor suppressor and known inhibitor of NF-κB activity, and MIF significantly induced NF-κB activation...
March 17, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28318046/hepatitis-c-virus-induced-ccl5-secretion-from-macrophages-activates-hepatic-stellate-cells
#8
Reina Sasaki, Pradip B Devhare, Robert Steele, Ranjit Ray, Ratna B Ray
Hepatitis C virus (HCV) mediated chronic liver disease is a serious health problem around the world, and often causes fibrosis/cirrhosis, and hepatocellular carcinoma (HCC). The mechanism of liver disease progression during HCV infection is still unclear, although inflammation is believed to be an important player in disease pathogenesis. We previously reported that macrophages including Kupffer cells exposed to HCV induce proinflammatory cytokines. These secreted cytokines may activate hepatic stellate cells (HSCs) towards fibrosis...
March 20, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/28317284/mir-320a-regulates-high-mobility-group-box-1%C3%A2-expression-and-inhibits-invasion-and-metastasis-in-hepatocellular-carcinoma
#9
Guixiang Lv, Mingjuan Wu, Meijie Wang, Xiaochen Jiang, Jingli Du, Kaili Zhang, Dongliang Li, Ning Ma, Yahui Peng, Lujing Wang, Lingyun Zhou, Weiming Zhao, Yufei Jiao, Xu Gao, Zheng Hu
BACKGROUND & AIMS: Several studies have shown that miR-320a induces apoptosis, inhibits cell proliferation, and affects cell cycle progression as a tumor suppressor in many cancers. However, the involvement of miR-320a in the invasion and metastasis of hepatocellular carcinoma (HCC) is still unknown. METHODS: Endogenous miR-320a and high mobility group box 1 (HMGB1) expressions were assayed by real-time PCR. Luciferase activities were measured using a dual-luciferase reporter assay system...
March 19, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/28315854/mutation-of-n-linked-glycosylation-in-epcam-affected-cell-adhesion-in-breast-cancer-cells
#10
Xue Liu, Jiujiao Gao, Yan Sun, Dandan Zhang, Tingjiao Liu, Qiu Yan, Xuesong Yang
EpCAM expression is elevated in breast cancer tissue, and correlates with the cancer metastasis and cell adhesion. Although EpCAM glycosylation is supposed to be associated with its function, the contribution of N-glycosylation to its function remains unclear. Here we analyzed cell adhesion ability of EpCAM in breast cancer cells. The results showed that EpCAM expression was associated with cell adhesion and N-glycosylation mutation of EpCAM decreased adhesion capacity. N-glycosylation mutation of EpCAM was correlated with lower levels of integrin β1 and fibronectin...
March 18, 2017: Biological Chemistry
https://www.readbyqxmd.com/read/28304404/interactions-between-th1-cells-and-tregs-affect-regulation-of-hepatic-fibrosis-in-biliary-atresia-through-the-ifn-%C3%AE-stat1-pathway
#11
Jie Wen, Ying Zhou, Jun Wang, Jie Chen, Wenbo Yan, Jin Wu, Junkai Yan, Kejun Zhou, Yongtao Xiao, Yang Wang, Qiang Xia, Wei Cai
Regulatory T cells (Tregs) and CD4(+) T helper (Th) cells have important roles in bile duct injury of biliary atresia (BA). However, their impacts on liver fibrosis are undefined. Between 2013 and 2016, 146 patients with various stages of BA were enrolled in this study. Peripheral blood, liver biopsy and lymph node samples were collected. Flow cytometry, magnetic cell sorting and immunostaining were used to characterize lymphocytes from BA patients. Deficiency of Tregs was observed along with increased Th1, Th2 and Th17 frequencies in the peripheral blood and livers of BA patients...
March 17, 2017: Cell Death and Differentiation
https://www.readbyqxmd.com/read/28302627/inhibition-of-smooth-muscle-%C3%AE-catenin-hinders-neointima-formation-after-vascular-injury
#12
Dario F Riascos-Bernal, Prameladevi Chinnasamy, Jordana N Gross, Vanessa Almonte, Lander Egaña-Gorroño, Dippal Parikh, Smitha Jayakumar, Liang Guo, Nicholas E S Sibinga
OBJECTIVE: Smooth muscle cells (SMCs) contribute to neointima formation after vascular injury. Although β-catenin expression is induced after injury, whether its function is essential in SMCs for neointimal growth is unknown. Moreover, although inhibitors of β-catenin have been developed, their effects on SMC growth have not been tested. We assessed the requirement for SMC β-catenin in short-term vascular homeostasis and in response to arterial injury and investigated the effects of β-catenin inhibitors on vascular SMC growth...
March 16, 2017: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/28300597/gli2-is-a-regulator-of-%C3%AE-catenin-and-is-associated-with-loss-of-e-cadherin-cell-invasiveness-and-long-term-epidermal-regeneration
#13
Eleni Pantazi, Emilios Gemenetzidis, Muy-Teck Teh, Sreekanth Vootukuri Reddy, Gary Warnes, Chris Evagora, Giuseppe Trigiante, Michael P Philpott
Uncontrolled HH/GLI and WNT/β-catenin signaling are important events in the genesis of many cancers including skin cancer and are often implicated in tumour progression, invasion and metastasis. However, due to the complexity and context-dependency of both pathways, little is known about HH and WNT interactions in human carcinogenesis. In the current study we provide evidence of HH/GLI2-WNT/β-catenin signaling crosstalk in human keratinocytes. Over-expression of GLI2ΔN in human keratinocytes resulted in cytoplasmic accumulation and nuclear relocalization of β-catenin in vitro and in 3D-organotypic cultures, accompanied by upregulation of WNT genes...
March 11, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28300207/both-drosophila-matrix-metalloproteinases-have-released-and-membrane-tethered-forms-but-have-different-substrates
#14
Kimberly S LaFever, Xiaoxi Wang, Patrick Page-McCaw, Gautam Bhave, Andrea Page-McCaw
Matrix metalloproteinases (MMPs) are extracellular proteases that can cleave extracellular matrix and alter signaling pathways. They have been implicated in many disease states, but it has been difficult to understand the contribution of individual MMPs, as there are over 20 MMPs in vertebrates. The vertebrate MMPs have overlapping substrates, they exhibit genetic redundancy and compensation, and pharmacological inhibitors are non-specific. In contrast, there are only two MMP genes in Drosophila, DmMmp1 and DmMmp2, which makes Drosophila an attractive system to analyze the basis of MMP specificity...
March 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28299729/expression-and-purification-of-matrix-metalloproteinases-in-escherichia-coli
#15
Krishna K Singh, Ruchi Jain, Harini Ramanan, Deepak K Saini
The MMP (matrix metalloproteinases) family of endopeptidases are involved in cleavage induced remodelling of the extracellular matrix including collagen, fibrinogen, elastin, and gelatin. Owing to their proteolytic activity which can cleave and degrade multiple intracellular substrates, the overexpression and purification of these proteins tends to be toxic. Here we describe a novel "matrix assisted refolding" protocol to overcome the technical challenges associated with overexpression and purification of full-length MMPs...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28299490/molecular-effects-of-fractional-ablative-erbium-yag-laser-treatment-with-multiple-stacked-pulses-on-standardized-human-three-dimensional-organotypic-skin-models
#16
Laurenz Schmitt, P M Amann, Y Marquardt, R Heise, K Czaja, P A Gerber, T Steiner, F Hölzle, Jens Malte Baron
The molecular changes in gene expression following ablative laser treatment of skin lesions, such as atrophic scars and UV-damaged skin, are not completely understood. A standardized in vitro model of human skin, to study the effects of laser treatment on human skin, has been recently developed. Therefore, the aim of the investigation was to examine morphological and molecular changes caused by fractional ablative erbium:YAG laser treatment on an in vitro full-thickness 3D standardized organotypic model of human skin...
March 15, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28294065/tetramethylpyrazine-facilitates-functional-recovery-after-spinal-cord-injury-by-inhibiting-mmp2-mmp9-and-vascular-endothelial-cell-apoptosis
#17
Jian-Zhong Hu, Xiao-Kai Wang, Yong Cao, Dong-Zhe Li, Tian-Ding Wu, Tao Zhang, Da-Qi Xu, Hong-Bin Lu
BACKGROUND: Spinal cord injury (SCI) is a major public health issue that leads to neurological dysfunctions and morbidities in patients. Tetramethylpyrazine (TMP) plays a neuroprotective role in SCI; however, the underlying mechanism has not been fully elucidated. Objective In the present study, we aimed to investigate the mechanisms and therapeutic effects of TMP on SCI. METHODS: A contusion SCI model was established that used a modified Allen's method. In the TMP group, TMP (200 mg/kg) was injected daily for 5 days post-injury, while in the Negative Control (NC) group, an equal volume of normal saline was injected...
March 13, 2017: Current Neurovascular Research
https://www.readbyqxmd.com/read/28292471/elevated-microrna-520g-in-pre-eclampsia-inhibits-migration-and-invasion-of-trophoblasts
#18
Liansheng Jiang, Anxiong Long, Longyi Tan, Mao Hong, Jingjing Wu, Leiming Cai, Qian Li
INTRODUCTION: Pre-eclampsia (PE) is a common and severe obstetric complication. MicroRNAs (miRs) have emerged as molecules that are associated with the disease. METHODS: Quantitative reverse transcription PCR (RT-qPCR) was used for serum miR-520g characterization from 19 severe pre-eclamptic and 19 normal pregnancies. In situ hybridation was adopted to localize microRNA-520g (miR-520g). Migration and invasion of HTR-8/SVneo cells were evaluated after miR-520g mimic treatment with transwell system...
March 2017: Placenta
https://www.readbyqxmd.com/read/28289901/overexpression-of-rsf-1-correlates-with-poor-survival-and-promotes-invasion-in-non-small-cell-lung-cancer
#19
Xiuwei Zhang, Lin Fu, Dongwei Xue, Xiupeng Zhang, Fengxia Hao, Lingling Xie, Jiani He, Junda Gai, Yuhui Liu, Hongtao Xu, Qingchang Li, Enhua Wang
Rsf-1 (HBXAP) was recently reported to play roles in tumorigenesis and tumor progression. There have been many reports referred to Rsf-1 overexpression in various cancers and associated with the malignant behavior of cancer cells. However, the molecular mechanism of Rsf-1 in non-small cell lung cancer aggressiveness remains ambiguous. In the present study, we found that there was a significant association between Rsf-1 overexpression and poor overall survival (p = 0.028) in lung cancer. Furthermore, knockdown of Rsf-1 expression in H1299 and H460 cells with high endogenous Rsf-1 expression inhibited cell migration and invasion and downregulated MMP2 expression and nuclear levels of NF-κB...
March 13, 2017: Virchows Archiv: An International Journal of Pathology
https://www.readbyqxmd.com/read/28288874/loss-of-exosomal-mir-320a-from-cancer-associated-fibroblasts-contributes-to-hcc-proliferation-and-metastasis
#20
Zhuochao Zhang, Xiao Li, Wei Sun, Shuqiang Yue, Jingyue Yang, Junjie Li, Ben Ma, Jianlin Wang, Xisheng Yang, Meng Pu, Bai Ruan, Ge Zhao, Qike Huang, Lin Wang, Kaishan Tao, Kefeng Dou
Cancer-associated fibroblasts (CAFs) play a pivotal role in regulating tumour progression. Therefore, understanding how CAFs communicate with hepatocellular carcinoma (HCC) is crucial for HCC therapy. Recently, exosomes have been considered an important "messenger" between cells. In this study, we performed microRNA (miRNA) sequencing of exosomes derived from CAFs and corresponding para-cancer fibroblasts (PAFs) of HCC patients. We found a significant reduction in the miR-320a level in CAF-derived exosomes...
March 10, 2017: Cancer Letters
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