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https://www.readbyqxmd.com/read/28819302/generation-and-intracellular-trafficking-of-a-polysialic-acid-carrying-fragment-of-the-neural-cell-adhesion-molecule-ncam-to-the-cell-nucleus
#1
Nina Westphal, Gabriele Loers, David Lutz, Thomas Theis, Ralf Kleene, Melitta Schachner
Polysialic acid (PSA) and its major protein carrier, the neural cell adhesion molecule NCAM, play important roles in many nervous system functions during development and in adulthood. Here, we show that a PSA-carrying NCAM fragment is generated at the plasma membrane by matrix metalloproteases and transferred to the cell nucleus via endosomes and the cytoplasm. Generation and nuclear import of this fragment in cultured cerebellar neurons is induced by a function-triggering NCAM antibody and a peptide comprising the effector domain (ED) of myristoylated alanine-rich C kinase substrate (MARCKS) which interacts with PSA within the plane of the plasma membrane...
August 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28818073/role-of-the-lncrna-abhd11-as1-in-the-tumorigenesis-and-progression-of-epithelial-ovarian-cancer-through-targeted-regulation-of-rhoc
#2
Dan-Dan Wu, Xi Chen, Kai-Xuan Sun, Li-Li Wang, Shuo Chen, Yang Zhao
BACKGROUND: There is increasing evidence in support of the role of lncRNAs in tumor cell proliferation, differentiation and apoptosis. METHODS: We examined the expression of the lncRNA ABHD11-AS1 in epithelial ovarian cancer (EOC) tissues and normal ovarian tissues by real-time quantitative PCR (qRT-PCR). After inducing ABHD11-AS1 downregulation by small interfering RNA (siRNA) or ABHD11-AS1 overexpression by plasmid transfection, we examined the EOC cell phenotypes and expression of related molecules...
August 17, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28817313/ccr7-mediates-tgf-%C3%AE-1-induced-human-malignant-glioma-invasion-migration-and-epithelial-mesenchymal-transition-by-activating-mmp2-9-through-the-nuclear-factor-kappab-signaling-pathway
#3
Yanyan Zheng, Yiting Miu, Xiaokai Yang, Xiaoguo Yang, Meijia Zhu
Chemokine receptor 7 (CCR7) has emerged as an inducer of invasion, migration, and epithelial-mesenchymal transition (EMT) in cancer. In this research, human malignant glioma cells were stimulated with transforming growth factor beta 1 (TGF-β1) and siCCR7. The data show that CCR7 mediates TGF-β1-induced EMT, migration, and invasion in U251 and U87 cells and that these effects of TGF-β1 were reversed by treatment with siCCR7 or a CCR7 neutralizing antibody. Importantly, the TGF-β1-mediated increase in nuclear factor kappaB (NF-κB) activity in human glioma cells was reduced by treatment with siCCR7 or a CCR7 neutralizing antibody...
August 17, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28812311/nad-replenishment-with-nicotinamide-mononucleotide-protects-blood-brain-barrier-integrity-and-attenuates-delayed-tpa-induced-haemorrhagic-transformation-after-cerebral-ischemia
#4
Chun-Chun Wei, Yuan-Yuan Kong, Xia-Hua, Guo-Qiang Li, Si-Li Zheng, Ming-He Cheng, Pei Wang, Chao-Yu Miao
BACKGROUND AND PURPOSE: Tissue plasminogen activator (tPA) is the only approved pharmacological therapy for acute brain ischemia; however, a major limitation of tPA is the haemorrhagic transformation followed by tPA treatment. Here, we determine whether nicotinamide mononucleotide (NMN), a key intermediate of nicotinamide adenine dinucleotide biosynthesis, would affect tPA-induced haemorrhagic transformation. EXPERIMENTAL APPROACH: Middle cerebral artery occlusion (MCAO) was achieved in CD1 mice by introducing a filament to the left MCA for 5 hours...
August 15, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28811128/a-newly-synthesized-ligustrazine-stilbene-derivative-inhibits-pdgf-bb-induced-vascular-smooth-muscle-cell-phenotypic-switch-and-proliferation-via-delaying-cell-cycle-progression
#5
Chunlian Peng, Siming Zhang, Haixin Liu, Yanxiao Jiao, Guifa Su, Yan Zhu
Vascular Smooth muscle cells (VSMCs) possess remarkable phenotype plasticity that allows it to rapidly adapt to fluctuating environmental cues, including the period of development and progression of vascular diseases such as atherosclerosis and restenosis subsequent to vein grafting or coronary intervention. Although VSMC phenotypic switch is an attractive target, there is no effective drug so far. Using rat aortic VSMCs, we investigate the effects of Ligustrazine and its synthetic derivatives on platelet-derived growth factor-BB (PDGF-BB) induced proliferation and phenotypic switch by a cell image-based screening of 60 Ligustrazine stilbene derivatives...
August 12, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28810927/stat3-mediated-upregulation-of-lncrna-hoxd-as1-as-a-cerna-facilitates-liver-cancer-metastasis-by-regulating-sox4
#6
Hui Wang, Xisong Huo, Xin-Rong Yang, Jia He, Lijun Cheng, Na Wang, Xuan Deng, Haojie Jin, Ning Wang, Cun Wang, Fangyu Zhao, Jingyuan Fang, Ming Yao, Jia Fan, Wenxin Qin
BACKGROUND: Several of the thousands of human long noncoding RNAs (lncRNAs) have been functionally characterized, yet their potential involvement in hepatocellular carcinoma (HCC) remains poorly understood. METHODS: LncRNA-HOXD-AS1 was identified by microarray and validated by real-time PCR. The clinicopathological significance of HOXD-AS1 was analyzed by Kaplan-Meier method. Chromatin immunoprecipitation was conducted to examine the mechanism of HOXD-AS1 upregulation...
August 14, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28800788/mir-204-regulates-cell-proliferation-and-invasion-by-targeting-ephb2-in-human-cervical-cancer
#7
Shanhong Duan, Ali Wu, Zhengyu Chen, Yarong Yang, Liying Liu, Qi Shu
MicroRNAs (miRNAs) are small non-coding RNAs that involved in human carcinogenesis and progression. miR-204 has been reported to be a tumor suppressor in several cancer types. However, the function and underlying molecular mechanism of miR-204 in cervical cancer (CC) are still unclear. In the present study, the expression level of miR-204 was measured using qRT-PCR method in 30 paired CC clinical samples and 6 CC cell lines. We found that the expression of miR-204 was significantly down-regulated in CC tissues and cell lines compared to normal cervical tissues and cell line...
August 11, 2017: Oncology Research
https://www.readbyqxmd.com/read/28800315/mir-99a-suppresses-proliferation-migration-and-invasion-of-esophageal-squamous-cell-carcinoma-cells-through-inhibiting-the-igf1r-signaling-pathway
#8
Li-Li Mei, Yun-Tan Qiu, Meng-Bing Huang, Wen-Jun Wang, Jie Bai, Zhi-Zhou Shi
miR-99a is down-regulated in esophageal squamous cell carcinoma (ESCC), however the role and underlying mechanism are still unknown. We aim to explore the role and mechanism of miR-99a down-regulation in ESCC. The expression of miR-99a in ESCC tissues and cell lines was detected by Human miRNA Microarrays and Real-time PCR. The effects of miR-99a on cell proliferation, migration and invasion were determined by Cell Counting Kit-8 (CCK-8) assay, transwell migration and invasion assay. Target gene of miR-99a were analyzed by target prediction software and validated by Real-time PCR and Western blotting assay...
July 31, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28797712/estrogen-receptor-beta-as-epigenetic-mediator-of-mir-10b-and-mir-145-in-mammary-cancer
#9
Zoi Piperigkou, Marco Franchi, Martin Götte, Nikos K Karamanos
Even though the role of estrogen receptor alpha (ERα) in the modulation of breast cancer cells' behavior is thoroughly studied, the biological functions of its isoform, ERβ, are less elucidated. The suppression of ERβ in the aggressive ERα-negative MDA-MB-231 breast cancer cells resulted in the inhibition of epithelial to mesenchymal transition (EMT) and major changes in the basic functional properties and expression levels of certain matrix components of breast cancer cells. This arrest in metastatic potential of breast cancer cells suggests the contribution of ERβ in the induction of a more aggressive phenotype in MDA-MB-231 breast cancer cells...
August 8, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28797051/prenylated-flavonoid-morusin-protects-against-tnbs-induced-colitis-in-rats
#10
Zora Vochyánová, Marie Pokorná, Dominik Rotrekl, Václav Smékal, Petr Fictum, Pavel Suchý, Jan Gajdziok, Karel Šmejkal, Jan Hošek
Morusin is a prenylated flavonoid isolated from the root bark of Morus alba. Many studies have shown the ability of flavonoids to act as anti-inflammatory agents. The aim of this study was to evaluate the effect of morusin on experimentally colitis induced by 2,4,6-trinitrobenzensulfonic acid in Wistar rats and to compare it with sulfasalazine, a drug conventionally used in the treatment of inflammatory bowel disease. Morusin was administered by gavage at doses of 12.5, 25, or 50 mg/kg/day for five days. The colonic tissue was evaluated macroscopically, histologically, and by performing immunodetection and zymographic analysis to determine the levels of antioxidant enzymes [superoxide dismutase (SOD) and catalase (CAT)], interleukin (IL)-1β, and transforming growth factor (TGF)-β1 and the activities of matrix metalloproteinases (MMP) 2 and 9...
2017: PloS One
https://www.readbyqxmd.com/read/28795476/ouabain-impairs-cell-migration-and-invasion-and-alters-gene-expression-of-human-osteosarcoma-u-2-os-cells
#11
Yung-Luen Shih, Man-Kuan Au, Ko-Lin Liu, Ming- Yang Yeh, Ching-Hsiao Lee, Mei-Hui Lee, Hsu-Feng Lu, Jiun-Long Yang, Rick Sai-Chuen Wu, Jing-Gung Chung
Ouabain, the specific Na(+) /K(+) -ATPase blocker, has biological activity including anti-proliferative and anti-metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration and invasion in human osteosarcoma U-2 OS cells. Thus, we investigated the effect of ouabain on the cell migration and invasion of human osteosarcoma U-2 OS cells. Results indicated that ouabain significantly decreased the percentage of viable cells at 2.5-5.0 μM, thus, we selected 0.25-1.0 μM for inhibiting studies...
August 10, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28782183/pectin-nanocoating-reduces-proinflammatory-fibroblast-response-to-bacteria
#12
A Mieszkowska, J Folkert, T Gaber, K Miksch, K Gurzawska
Implant failures are primarily related to bacterial infections and inflammation. Nanocoating of implant devices with organic molecules is a method used for improving their integration into host tissues and limiting inflammation. Bioengineered plant-derived rhamnogalacturonan-Is (RG-Is) from pectins improve tissue regeneration and exhibit anti-inflammatory properties. Therefore, the aim of this study is to evaluate the in vitro effect of RG-I nanocoating on human gingival primary fibroblast (HGF) activity and proinflammatory response following Porphyromonas gingivalis (P...
August 7, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28780604/mir-338-5p-promotes-glioma-cell-invasion-by-regulating-tshz3-and-mmp2
#13
Yanyan Li, Yulun Huang, Zhenyu Qi, Ting Sun, Youxin Zhou
This study was designed to examine differential expression of miR-338-5p in gliomas and the role of miR-338-5p in glioma cell invasion via its potential target gene TSHZ3 encoding Teashirt zinc finger homobox 3, predicted by bioinformatics, and matrix metallopeptidase 2 (MMP2), the key pro-invasive protease overexpressed in gliomas. Quantitative real-time reverse transcription PCR (qRT-PCR) and Spearman correlation analysis were used to determine differential expressions of miR-338-5p and TSHZ3 in astrocytic gliomas of different grades (n = 35) and glioblastoma cell lines (U87 and U251) in comparison to non-neoplastic brain (NNB) tissues (n = 6)...
August 5, 2017: Cellular and Molecular Neurobiology
https://www.readbyqxmd.com/read/28777298/the-sglt2-inhibitor-luseogliflozin-rapidly-normalizes-aortic-mrna-levels-of-inflammation-related-but-not-lipid-metabolism-related-genes-and-suppresses-atherosclerosis-in-diabetic-apoe-ko-mice
#14
Yusuke Nakatsu, Hiroki Kokubo, Batmunkh Bumdelger, Masao Yoshizumi, Takeshi Yamamotoya, Yasuka Matsunaga, Koji Ueda, Yuki Inoue, Masa-Ki Inoue, Midori Fujishiro, Akifumi Kushiyama, Hiraku Ono, Hideyuki Sakoda, Tomoichiro Asano
Recent clinical studies have revealed the treatment of diabetic patients with sodium glucose co-transporter2 (SGLT2) inhibitors to reduce the incidence of cardiovascular events. Using nicotinamide and streptozotocin (NA/STZ) -treated ApoE KO mice, we investigated the effects of short-term (seven days) treatment with the SGLT2 inhibitor luseogliflozin on mRNA levels related to atherosclerosis in the aorta, as well as examining the long-term (six months) effects on atherosclerosis development. Eight-week-old ApoE KO mice were treated with NA/STZ to induce diabetes mellitus, and then divided into two groups, either untreated, or treated with luseogliflozin...
August 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28775096/repetitive-intradermal-bleomycin-injections-evokes-t-helper-cell-2-cytokine-driven-pulmonary-fibrosis
#15
Brijendra Singh, Rajesh K Kasam, Vishwaraj Sontake, Thomas A Wynn, Satish K Madala
IL-4 and IL-13 are major T helper cell (Th) 2 cytokines implicated in the pathogenesis of several lung diseases, including pulmonary fibrosis. In this study, using a novel repetitive intradermal bleomycin model in which mice develop extensive lung fibrosis and a progressive decline in lung function compared to saline-treated control mice, we investigated profibrotic functions of Th2 cytokines. To determine the role of IL-13 signaling in the pathogenesis of bleomycin-induced pulmonary fibrosis, wild-type, IL-13, and IL-4Rα-deficient mice were treated with bleomycin, and lungs were assessed for changes in lung function and pulmonary fibrosis...
August 3, 2017: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/28770666/the-influence-of-vitamin-d-on-m1-and-m2-macrophages-in-patients-with-crohn-s-disease
#16
Serge Dionne, Carl-Frederic Duchatelier, Ernest G Seidman
Defective bacterial clearance by macrophages plays an important role in Crohn's disease (CD). Phenotypes and functions of inflammatory M1 and anti-inflammatory M2 have not been studied in CD. Vitamin D supplementation reduces the severity of CD by unclear mechanisms. We studied macrophage characteristics in CD and controls and the effects of 1,25 vitamin D (1,25D). PBMC were isolated from CD patients and controls. M1 and M2 were generated by culturing of monocytes with GM-CSF and M-CSF, respectively. CD M1 and M2 showed normal phagocytosis and chemotaxis to CCL2 and fMLP...
August 2017: Innate Immunity
https://www.readbyqxmd.com/read/28765941/b7-h3-is-related-to-tumor-progression-in-ovarian-cancer
#17
Jingjing Zhang, Lu Liu, Sai Han, Yi Li, Qiuhong Qian, Qianqian Zhang, Hui Zhang, Ziyan Yang, Youzhong Zhang
B7-H3, a co-stimulatory molecule, has been found expressed in ovarian cancer, but its role and mechanism is not clear. In this study, we further verified the expression of B7-H3 in ovarian carcinoma and normal epithelial ovarian tissues. Three ovarian cancer cell lines, A2780, SKOV3 and HO8910 were selected to explore the effects of B7-H3 on proliferation, apoptosis, migration and invasion. We found that B7-H3 was mainly located in the cytoplasm of ovarian cancer cells as determined by immunofluorescence staining...
July 31, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28756371/il-27-induces-a-pro-inflammatory-response-in-human-fetal-membranes-mediating-preterm-birth
#18
Nanlin Yin, Hanbing Wang, Hua Zhang, Huisheng Ge, Bing Tan, Yu Yuan, Xiaofang Luo, David M Olson, Philip N Baker, Hongbo Qi
Inflammation at the maternal-fetal interface has been shown to be involved in the pathogenesis of preterm birth. Interleukin 27 (IL-27), a heterodimeric cytokine, is known to mediate an inflammatory response in some pregnancy complications. In this study, we aimed to determine whether IL-27 could induce an inflammatory reaction at the maternal-fetal interface that would mediate the onset of preterm birth. We found elevated expression of IL-27 in human peripheral serum and elevated expression of its specific receptor (wsx-1) on fetal membranes in cases of preterm birth...
July 26, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/28754560/correlation-of-cellular-factors-and-differential-scrapie-prion-permissiveness-in-ovine-microglia
#19
Kelcey D Dinkel, David A Schneider, Juan F Muñoz-Gutiérrez, Valerie R McElliott, James B Stanton
Prion diseases are fatal neurodegenerative disorders by which the native cellular prion protein (PrP(C)) is misfolded into an accumulating, disease-associated isoform (PrP(D)). To improve the understanding of prion pathogenesis and develop effective treatments, it is essential to elucidate factors contributing to cellular permissiveness. We previously isolated five clones from an immortalized subline of ovine microglia, two of which had demonstrated differential permissiveness to a natural isolate of sheep scrapie and distinct transcriptomic profiles...
July 25, 2017: Virus Research
https://www.readbyqxmd.com/read/28750740/lncrna-odrul-contributes-to-osteosarcoma-progression-through-the-mir-3182-mmp2-axis
#20
Kun-Peng Zhu, Xiao-Long Ma, Chun-Lin Zhang
Recent findings have shown that lncRNA dysregulation is involved in many cancers, including osteosarcoma (OS). In a previous study, we reported a novel lncRNA, ODRUL, that could promote doxorubicin resistance in OS. We now report the function and underlying mechanism of ODRUL in regulating OS progression. We show that ODRUL is upregulated in OS tissues and cell lines and correlates with poor prognosis. ODRUL knockdown significantly inhibits OS cell proliferation, migration, invasion, and tumor growth in vitro and in vivo by decreasing matrix metalloproteinase (MMP) expression...
July 5, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
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