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Ichitaro Abe, Yasushi Teshima, Hidekazu Kondo, Haruka Kaku, Shintaro Kira, Yuki Ikebe, Shotaro Saito, Akira Fukui, Tetsuji Shinohara, Kunio Yufu, Mikiko Nakagawa, Naoki Hijiya, Masatsugu Moriyama, Tatsuo Shimada, Shinji Miyamoto, Naohiko Takahashi
BACKGROUND: Epicardial adipose tissue (EAT) is associated with atrial fibrillation (AF), but the underlying mechanisms remain to be fully elucidated. OBJECTIVE: Using human left atrial appendage (LAA) samples, we examined the interactive relationship between the EAT profile and atrial myocardial fibrosis through histological and biochemical analyses. METHODS: LAA samples were obtained from 59 consecutive AF patients during cardiovascular surgery...
June 13, 2018: Heart Rhythm: the Official Journal of the Heart Rhythm Society
Lali Tsiklauri, Janina Werner, Marian Kampschulte, Klaus W Frommer, Lucija Berninger, Martina Irrgang, Kristina Glenske, Dirk Hose, Thaqif El Khassawna, Jörn Pons-Kühnemann, Stefan Rehart, Sabine Wenisch, Ulf Müller-Ladner, Elena Neumann
OBJECTIVES: Age-related bone loss is associated with bone marrow adiposity. Adipokines (e.g. visfatin, resistin, leptin) are adipocyte-derived factors with immunomodulatory properties and might influence differentiation of bone marrow-derived mesenchymal stem cells (MSC) in osteoarthritis (OA) and osteoporosis. Thus, the presence of adipokines and MMPs in bone marrow and their effects on MSC differentiation were analyzed. METHODS: MSC and RNA were isolated from femoral heads after hip replacement surgery of OA or osteoporotic femoral neck fracture (FF) patients...
June 13, 2018: Osteoarthritis and Cartilage
Santosh D Bhosale, Robert Moulder, Mikko S Venäläinen, Juhani S Koskinen, Niina Pitkänen, Markus T Juonala, Mika A P Kähönen, Terho J Lehtimäki, Jorma S A Viikari, Laura L Elo, David R Goodlett, Riitta Lahesmaa, Olli T Raitakari
To evaluate the presence of serum protein biomarkers associated with the early phases of formation of carotid atherosclerotic plaques, label-free quantitative proteomics analyses were made for serum samples collected as part of The Cardiovascular Risk in Young Finns Study. Samples from subjects who had an asymptomatic carotid artery plaque detected by ultrasound examination (N = 43, Age = 30-45 years) were compared with plaque free controls (N = 43) (matched for age, sex, body weight and systolic blood pressure)...
June 15, 2018: Scientific Reports
Xiaoqian Wang, Bing Yang, Yuanping She, Yuan Ye
Ovarian cancer is one of the most common gynecologic malignancy with poor prognosis. Recently, long noncoding RNAs (lncRNAs) have been identified as key regulators in cancer development. The current study investigated the role of lncRNA P73 antisense RNA 1T (TP73-AS1) in ovarian cancer. Quantitative real-time polymerase chain reaction determined the expression levels of TP-73AS1, matrix metallopeptidases (MMPs) messenger RNA. Cell proliferative ability, cell invasion, and migration were CCK-8 and colony formation, and transwell invasion and migration assays, respectively...
June 15, 2018: Journal of Cellular Biochemistry
Shu Yang, Mengyang Liu, Yuanli Chen, Chuanrui Ma, Lipei Liu, Buchang Zhao, Yong Wang, Xiaoju Li, Yan Zhu, Xiumei Gao, Deling Kong, Yajun Duan, Jihong Han, Xiaoxiao Yang
NaoXinTong Capsule (NXT), a Chinese medicine, is currently used to treat patients with cardiovascular and cerebrovascular diseases. Clinical observations indicate its anti-diabetic functions with unclear mechanisms. Herein, we report the effect of NXT on diabetic nephropathy (DN). Type 2 diabetic db/db mice were treated with NXT for 14 weeks. In the course of treatment, NXT reduced diabetes-increased glucose levels and improved renal functions. At the end of treatment, we found that NXT ameliorated serum lipid profiles and other biochemical parameters...
June 14, 2018: Scientific Reports
Chunli Song, Huanhuan Yang, Xiaochao Song, Deming Li, Xinli Li
OBJECTIVE: To investigate the effects of apple polyphenol on cell proliferation and migration of breast cancer cell line MDA-MB-231, and explore the potential mechanism. METHODS: The breast cancer cells in the logarithmic phase were treated with 0, 50, 100, 200, 400 and 800 μg/m L of apple polyphenol for 24, 48 and 72 h, then Trypan blue staining was employed to detect cell vitality; CCK8 kit was used to determine cell growth and proliferation; cell migration ability was observed by scratch experiment, and the change of proteins expression level was assessed by Western blot...
November 2017: Wei Sheng Yan Jiu, Journal of Hygiene Research
Zi-Wen Long, Jiang-Hong Wu, Cai- Hong, Ya-Nong Wang, Ye Zhou
Gastrointestinal stromal tumours (GIST) are the most common mesenchymal tumors of the gastrointestinal (GI) tract. In order to investigate a new treatment fot GIST, we hypothesized the effect of miR-374b targeting PTEN gene-mediated PI3K/Akt signal transduction pathway on proliferation and apoptosis of human gastrointestinal stromal tumor (GIST) cells. We obtained GIST tissues and adjacent normal tissues from 143 patients with GIST to measure the levels of miR- 374b, PTEN, PI3K, Akt, caspase9, Bax, MMP2, MMP9, ki67, PCNA, P53 and cyclinD1...
June 14, 2018: Molecules and Cells
Soo Kyoung Park, Soung Yong Jin, Sun Hee Yeon, Sung Bok Lee, Jun Xu, Young Hoon Yoon, Ki Sang Rha, Yong Min Kim
BACKGROUND: Nasal polyposis is characterized by persistent inflammation and remodeling in sinonasal mucosa. Toll-like receptor 9 (TLR9) is a DNA receptor of the innate immune system that plays a pivotal role in fibrosis and inflammatory responses. The aim of this study is to explore the expression, activity, and potential pathogenic role of TLR9 signaling in tissue remodeling in nasal polyp-derived fibroblasts (NPDFs). METHODS: Fibrotic and inflammatory responses elicited by type A CpG oligonucleotides were examined in the NPDFs by a combination of real-time quantitative polymerase chain reaction, Western blot analysis, enzyme-linked immunosorbent assay, and immunofluorescence staining...
June 14, 2018: International Forum of Allergy & Rhinology
Dong Young Kang, Nipin Sp, Doh Hoon Kim, Youn Hee Joung, Hyo Gun Lee, Young Min Park, Young Mok Yang
The major hallmarks of tumor progression are angiogenesis, migration and metastasis. Among the components of Rhodiola rosea, salidroside (p‑hydroxyphenethyl-β‑d-glucoside) is one of the most potent, and is present in all Rhodiola species. Recent data have revealed the anticancer effects of salidroside; however, the mechanism underlying its ability to inhibit tumor angiogenesis remains unknown. The present study aimed to analyze how salidroside affects major factors involved in breast cancer, and to elucidate its ability to inhibit angiogenesis and invasion...
June 5, 2018: International Journal of Oncology
Ye Wu, Lei Tian, Yongle Xu, Minhong Zhang, Shengqing Xiang, Jianguo Zhao, Zhenxia Wang
C-X-C chemokine receptor type 7 (CXCR7) is reported to be overexpressed in tumor endothelial cells (TECs), which are the primary target cells of antivascular chemotherapy. However, the role of CXCR7 in TECs is not fully understood. In the present study, CXCR7 expression was inhibited in TECs derived from hepatocellular carcinoma (HCC) using short hairpin (sh)RNA plasmids to investigate the role of CXCR7 in the regulation of migration and invasion of TECs as well as its underlying mechanisms. The data showed that the downregulation of CXCR7 significantly inhibited the migration and invasion of TECs...
May 31, 2018: Molecular Medicine Reports
Brett T Chiquet, Qiuping Yuan, Eric C Swindell, Lorena Maili, Robert Plant, Jeffrey Dyke, Ryan Boyer, John F Teichgraeber, Matthew R Greives, John B Mulliken, Ariadne Letra, Susan H Blanton, Jacqueline T Hecht
Orofacial development is a multifaceted process involving tightly regulated genetic signaling networks, that when perturbed, lead to orofacial abnormalities including cleft lip and/or cleft palate. We and others have shown an association between the cysteine-rich secretory protein LCCL domain containing 2 (CRISPLD2) gene and nonsyndromic cleft lip with or without cleft palate (NSCLP). Further, we demonstrated that knockdown of Crispld2 in zebrafish alters neural crest cell migration patterns resulting in abnormal jaw and palate development...
June 13, 2018: European Journal of Human Genetics: EJHG
Yi Zhang, Ran Tao, Shan-Shan Wu, Cui-Cui Xu, Jie-Ling Wang, Jie Chen, Yong-Sheng Yu, Zheng-Hao Tang, Xiao-Hua Chen, Guo-Qing Zang
BACKGROUND: Many tripartite motif (TRIM) family proteins have been reported to be of great importance in the initiation and progression in hepatocellular carcinoma (HCC). However, the biological role and regulatory mechanism of tripartite motif containing 52 (TRIM52) in HCC development and progression are poorly defined. METHODS: Immunohistochemistry (IHC), quantitative real-time PCR (qRT-PCR) or Western blot analysis was used to detect TRIM52, p21, matrix metalloproteinase 2 (MMP2), protein phosphatase, Mg2+ /Mn2+ dependent 1A (PPM1A), p-Smad2/3 and Smad2/3 levels in HCC tissues and cell lines...
June 13, 2018: Journal of Experimental & Clinical Cancer Research: CR
Guilin Meng, Xiulin Meng, Xiaoye Ma, Gengping Zhang, Xiaolin Hu, Aiping Jin, Yanxin Zhao, Xueyuan Liu
Alzheimer's disease (AD) is an increasing concern in human health. Despite significant research, highly effective drugs to treat AD are lacking. The present study describes the text mining process to identify drug candidates from a traditional Chinese medicine (TCM) database, along with associated protein target mechanisms. We carried out text mining to identify literatures that referenced both AD and TCM and focused on identifying compounds and protein targets of interest. After targeting one potential TCM candidate, corresponding protein-protein interaction (PPI) networks were assembled in STRING to decipher the most possible mechanism of action...
2018: Frontiers in Neuroinformatics
Xiao-Lan Ai, Fang Yao, Xiao-Jing Wang, Dong-Bei Duan, Ke Li, Zi-You Hu, Guo Yin, Mei Wang, Bing-Yi Wu
OBJECTIVE: To investigate the role of allograft inflammatory factor-1 (AIF-1) in colorectal cancer (CRC) progression and explore the possible mechanism. METHODS: The expression levels of AIF-1 in 70 CRC tissues and paired adjacent tissues were detected using immunohistochemistry and Western blotting, and the correlation of AIF-1 expression with the clinicopathological features of the patients was analyzed. In the CRC cell line SW480, the functional role of AIF-1 in regulating tumor progression was investigated by transfecting the cells with an AIF-1-overexpressing plasmid (AIF-1) and a negative control plasmid (NC)...
May 20, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
Jiamiao Hu, Shaoling Lin, Jim Junhui Huang, Peter C K Cheung
The anti-angiogenic activities of two structurally similar phenolics protocatechuic acid (PA) and syringic acid (SA) were investigated. In vitro study using HUVECs demonstrated that both PA and SA (at 25 μM) significantly (p < 0.05) inhibited VEGF-induced cell proliferation by 22.68 ± 5.6% and 21.93 ± 2.0%, respectively; cell migration by 50.04 ± 3.3% and 39.72 ± 4.7%, respectively; cell invasion by 44.16 ± 4.23% and 51.90 ± 2.73%, respectively; and cellular ROS generation by 11.48 ± 6.32% and 21...
June 10, 2018: Journal of Agricultural and Food Chemistry
Lin Li, Debo Yun, Yuan Zhang, Yihao Tao, Qiang Tan, Fei Qiao, Bo Luo, Yi Liu, Runjin Fan, Jishu Xian, Anyong Yu
BACKGROUND AND PURPOSE: The blood-brain barrier (BBB) disruption and the following development of brain edema, is the most life-threatening secondary injury after intracerebral hemorrhage (ICH). This study is to investigate a potential role and mechanism of JWH133, a selected cannabinoid receptor type2 (CB2R) agonist, on protecting blood-brain barrier integrity after ICH. METHODS: 192 adult male Sprague-Dawley (SD) rats were randomly divided into Sham; ICH+Vehicle; ICH+JWH 1...
June 7, 2018: Brain Research
Zhi Jiang, Huan Zhang, Chunliang Liu, Jun Yin, Shan Tong, Junxing Lv, Shaohua Wei, Shiliang Wu
β1,3-N-acetylglucosaminyltransferase (β3GnT8) and β3GnT2 are key enzymes that catalyzes the formation of polylactosamine glycan structures by transferring GlcNAc to tetra-antennary β1-6-branched N -glycan and it also has an important effect on the progression of various types of human cancer. They have been reported to participate in tumor invasion and metastasis by regulating the expression of matrix metalloproteinases (MMPs), CD147, and polylactosamine. However, whether β3GnT8 and β3GnT2 play a role in colorectal cancer and, if so, the underlying mechanisms remain unclear...
2018: Frontiers in Physiology
Hui Li, Yu Zhang, Jun Hai, Jixin Wang, Bei Zhao, Lixue Du, Xilin Geng
Tripartite motif (TRIM) 31, a member of the TRIM protein family, contributes to a wide range of biological processes and its altered expression exerts a non-negligible effect on multiple pathological conditions such as immunological disorders and retroviral protective activity. Recently, increasing evidence has demonstrated an important role of TRIM31 in the development of various cancers. However, the role of TRIM31 in gallbladder cancer (GBC) remains unclear. In this study, we showed that TRIM31 was elevated in GBC tissues and cell lines and associated with the clinicopathological features of GBC patients...
July 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Wei Qu, Dichen Li, Yufei Wang, Qining Wu, Dingjun Hao
BACKGROUND Radioresistance restricts the application of radiotherapy in human osteosarcoma (OS). This study investigated the molecular mechanism of radioresistance in OS, which may provide clues to finding ideal targets for genetic therapy. MATERIAL AND METHODS The human OS cell line MG63 was employed as parent cells. After repeat low-dose X-ray irradiation of MG63, the radioresistant OS cell line MG63R was produced. Colony formation assay was used to assess the radioresistance. Cell viability was evaluated by CCK-8 assay...
June 4, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Tong Liu, Ling Xu, Chengfen Wang, Kan Chen, Yujing Xia, Jingjing Li, Sainan Li, Liwei Wu, Jiao Feng, Shizan Xu, Wenwen Wang, Xiya Lu, Xiaoming Fan, Wenhui Mo, Yingqun Zhou, Yan Zhao, Chuanyong Guo
BACKGROUND AND AIM: Liver fibrosis is a worldwide clinical challenge during the progression of chronic liver disease to liver cirrhosis. Shikonin is extracted from the root of Lithospermum erythrorhizon with antioxidant, anti-inflammatory, anticancer, and wound healing properties. The study aim to investigate the protective effect of shikonin on liver fibrosis and its underlying mechanism. METHODS: Two liver fibrosis models were established in male C57 mice by intraperitoneal injection of CCl4 or bile duct ligation (BDL)...
June 4, 2018: Journal of Gastroenterology and Hepatology
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