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https://www.readbyqxmd.com/read/28625714/proteomic-analysis-of-seminal-plasma-from-locally-adapted-curraleiro-p%C3%A3-duro-bulls-bos-taurus-identifying-biomarkers-involved-in-sperm-physiology-in-endangered-animals-for-conservation-of-biodiversity
#1
E B Menezes, R V de Oliveira, M F van Tilburg, E A Barbosa, N V Nascimento, A L M C S Velho, F B Moreno, R A Moreira, A C O Monteiro-Moreira, G M C Carvalho, A F Ramos, E Memili, A A Moura
The present study was aimed at evaluating the seminal plasma proteins and sperm parameters of Curraleiro Pé-Duro bulls. Semen was collected from 10 bulls by electroejaculation, and sperm parameters were evaluated in fresh and frozen-thawed semen. Seminal plasma proteins were analyzed by 2-D SDS-PAGE and mass spectrophotometry. Tools in computational biology were used to generate bioinformatic knowledge and evaluate gene ontology, protein-protein interactions, phylogenetic trees and multiple sequence alignments...
May 29, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28620234/ezh2-mediated-epigenetic-silencing-of-timp2-promotes-ovarian-cancer-migration-and-invasion
#2
Xiaoqing Yi, Jianfeng Guo, Jing Guo, Si Sun, Ping Yang, Junjie Wang, Yuan Li, Lisha Xie, Jing Cai, Zehua Wang
Enhancer of zeste homolog 2 (EZH2) is often increased in malignant tumors and is involved in metastasis. EZH2 silences gene expression by tri-methylating the lysine 27 residue of histone H3 (H3K27me3). However, the mechanism underlying EZH2 promotion of ovarian cancer metastasis remains elusive. Here, we showed that EZH2 is up-regulated in ovarian cancer and is associated with tumor metastasis and poor survival by mRNA sequencing and microarray results from databases. Tissue microarray and immunohistochemistry results revealed that EZH2 was negatively correlated with the expression of tissue inhibitor of metalloproteinases 2 (TIMP2)...
June 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28570941/diet-dependent-retinoid-effects-on-liver-gene-expression-include-stellate-and-inflammation-markers-and-parallel-effects-of-the-nuclear-repressor-shp
#3
Meghan Maguire, Justin R Bushkofsky, Michele Campaigne Larsen, Yee Hoon Foong, Sherry A Tanumihardjo, Colin R Jefcoate
For mice, a maternal vitamin A (VA)-deficient diet initiated from midgestation (GVAD) produces serum retinol deficiency in mature offspring. We hypothesize that the effects of GVAD arise from preweaning developmental changes. We compare the effect of this GVAD protocol in combination with a postweaning high-fat diet (HFD) or high-carbohydrate diet (LF12). Each is compared to an equivalent VA-sufficient combination. GVAD extensively decreased serum retinol and liver retinol, retinyl esters, and retinoid homeostasis genes (Lrat, Cyp26b1 and Cyp26a1)...
April 19, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28552795/rhukgf-ameliorates-protease-anti-protease-imbalance-in-emphysematous-mice
#4
Sudhir Kotnala, Amit Tyagi, Jai Prakash Muyal
We attempted to elucidate the beneficial role of rHuKGF supplementation in the amelioration of protease/antiprotease imbalance and TGF-β1 signalling pathway leading to alveolar tissue maintenance in elastase induced emphysematous mice. Thirty two male C57BL mice were divided into four groups i.e. control, emphysema, therapy and rHuKGF only and were oropharyngeally instilled with saline/porcine pancreatic elastase/rHuKGF. Subsequently, lungs from mice were collected for histopathology and molecular biology studies...
May 25, 2017: Pulmonary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28537976/interest-of-urinary-timp-2-%C3%A3-igfbp-7-for-predicting-the-occurrence-of-acute-kidney-injury-after-cardiac-surgery-a-gray-zone-approach
#5
Toufic Finge, Sebastien Bertran, Claire Roger, Damien Candela, Bruno Pereira, Christopher Scott, Laurent Muller, Benjamin Louart, Jean-Yves Lefrant
BACKGROUND: This study assessed the ability of 3-hour postoperative urinary tissue inhibitor of metalloproteinases-2 × insulin-like growth factor binding protein-7 ([TIMP-2] × [IGFBP-7]) to predict postoperative acute kidney injury (AKI) in patients undergoing cardiopulmonary bypass during cardiac surgery. METHODS: Patients undergoing cardiac surgery with cardiopulmonary bypass were eligible for this study. Patients with initial chronic renal insufficiency requiring renal replacement therapy, patients <18 years of age, and parturients were not included...
May 19, 2017: Anesthesia and Analgesia
https://www.readbyqxmd.com/read/28498424/microrna-15b-promotes-proliferation-and-invasion-of-non%C3%A2-small-cell-lung-carcinoma-cells-by-directly-targeting-timp2
#6
Haowen Wang, Yu Zhan, Jingjing Jin, Chunhong Zhang, Wenfeng Li
MicroRNA-15b (miR-15b) plays an important role in tumor development and progression. miR-15b functions differently in various types of malignant tumors. However, the expression pattern and role of miR-15b in non-small cell lung cancer (NSCLC) have not been elucidated. In the present study, we investigated the effect of miR-15b on the occurrence and development of lung cancer and the underlying mechanism. Lung cancer cell lines A549 and LTEP-a-2 were transfected with miR-15b inhibitor or mimic, respectively...
April 26, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28492359/periodontitis-and-endothelial-dysfunction-periodontal-clinical-parameters-and-the-levels-of-salivary-markers-il-1%C3%AE-tnf-%C3%AE-mmp2-timp2-complex-and-nitric-oxide
#7
Marcela Faria Moura, Túlio Pinho Navarro, Tarcília Aparecida Silva, Luís Otávio Miranda Cota, Alcione Maria Soares Dutra Oliveira, Fernando Oliveira Costa
BACKGROUND: Periodontitis has been associated with a greater risk for arteriosclerotic cardiovascular disease (ACD). Endothelial dysfunction (ED) is a parameter of early ACD, and its association with periodontitis has rarely been investigated to date. The objective of this study was to evaluate the association between periodontitis and ED by means of periodontal clinical parameters and salivary markers IL-1β, TNF-α, nitric oxide-(NO) and MMP2/TIMP2 complex. METHODS: Forty-seven individuals were divided into two groups: 24 individuals with chronic periodontitis and 23 individuals without chronic periodontitis...
May 11, 2017: Journal of Periodontology
https://www.readbyqxmd.com/read/28471417/protease-expression-levels-in-prostate-cancer-tissue-can-explain-prostate-cancer-associated-seminal-biomarkers-an-explorative-concept-study
#8
Jochen Neuhaus, Eric Schiffer, Ferdinando Mannello, Lars-Christian Horn, Roman Ganzer, Jens-Uwe Stolzenburg
Previously, we described prostate cancer (PCa) detection (83% sensitivity; 67% specificity) in seminal plasma by CE-MS/MS. Moreover, advanced disease was distinguished from organ-confined tumors with 80% sensitivity and 82% specificity. The discovered biomarkers were naturally occurring fragments of larger seminal proteins, predominantly semenogelin 1 and 2, representing endpoints of the ejaculate liquefaction. Here we identified proteases putatively involved in PCa specific protein cleavage, and examined gene expression and tissue protein levels, jointly with cell localization in normal prostate (nP), benign prostate hyperplasia (BPH), seminal vesicles and PCa using qPCR, Western blotting and confocal laser scanning microscopy...
May 4, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28444820/casticin-impairs-cell-migration-and-invasion-of-mouse-melanoma-b16f10-cells-via-pi3k-akt-and-nf-%C3%AE%C2%BAb-signaling-pathways
#9
Yung-Luen Shih, Hsiao-Min Chou, Hsiu-Chen Chou, Hsu-Feng Lu, Yung-Lin Chu, Hung-Sheng Shang, Jing-Gung Chung
Casticin, a polymethoxyflavone, is one of the major active components obtained from Fructus viticis, which have been shown to have anticancer activities including induce cell apoptosis in human cancer cells. The aim of this study was to investigate the molecular mechanisms by which casticin inhibits cell migration and invasion of mouse melanoma B16F10 cells. Cell viability was examined by MTT assay and the results indicated that casticin decreased the total percentages of viable cells in dose-dependent manners...
April 26, 2017: Environmental Toxicology
https://www.readbyqxmd.com/read/28441400/a-genomics-approach-identifies-selective-effects-of-trans-resveratrol-in-cerebral-cortex-neuron-and-glia-gene-expression
#10
Gemma Navarro, Eva Martínez-Pinilla, Alejandro Sánchez-Melgar, Raquel Ortiz, Véronique Noé, Mairena Martín, Carlos Ciudad, Rafael Franco
The mode of action of trans-resveratrol, a promising lead compound for the development of neuroprotective drugs, is unknown. Data from a functional genomics study were retrieved with the aim to find differentially expressed genes that may be involved in the benefits provided by trans-resveratrol. Genes that showed a significantly different expression (p<0.05, cut-off of a two-fold change) in mice fed with a control diet or a control diet containing trans-resveratrol were different in cortex, heart and skeletal muscle...
2017: PloS One
https://www.readbyqxmd.com/read/28424512/human-umbilical-cord-plasma-proteins-revitalize-hippocampal-function-in-aged-mice
#11
Joseph M Castellano, Kira I Mosher, Rachelle J Abbey, Alisha A McBride, Michelle L James, Daniela Berdnik, Jadon C Shen, Bende Zou, Xinmin S Xie, Martha Tingle, Izumi V Hinkson, Martin S Angst, Tony Wyss-Coray
Ageing drives changes in neuronal and cognitive function, the decline of which is a major feature of many neurological disorders. The hippocampus, a brain region subserving roles of spatial and episodic memory and learning, is sensitive to the detrimental effects of ageing at morphological and molecular levels. With advancing age, synapses in various hippocampal subfields exhibit impaired long-term potentiation, an electrophysiological correlate of learning and memory. At the molecular level, immediate early genes are among the synaptic plasticity genes that are both induced by long-term potentiation and downregulated in the aged brain...
April 27, 2017: Nature
https://www.readbyqxmd.com/read/28423650/mir-425-5p-promotes-invasion-and-metastasis-of-hepatocellular-carcinoma-cells-through-scai-mediated-dysregulation-of-multiple-signaling-pathways
#12
Feng Fang, Tianqiang Song, Ti Zhang, Yunlong Cui, Gewen Zhang, Qingqing Xiong
MicroRNAs (miRNAs) play critical roles in hepatocellular carcinoma (HCC) progression and are key determinants of prognosis. In this study, we found that miR-425-5p was elevated in HCC and correlated with poor prognostic clinicopathological features and low post-operative long-term survival. Multivariate survival analysis indicated that miR-425-5p expression was an independent risk factor for overall and disease-free survival. Interestingly, miR-425-5p promoted invasion and metastasis by HCC cells, but not HCC cell proliferation or apoptosis in vitro...
May 9, 2017: Oncotarget
https://www.readbyqxmd.com/read/28392461/recombinant-egfr-mmp-2-bi-targeted-fusion-protein-markedly-binding-to-non-small-cell-lung-carcinoma-and-exerting-potent-therapeutic-efficacy
#13
Jian Xu, Yue Du, Xiu-Jun Liu, Bing-Yan Zhu, Sheng-Hua Zhang, Liang Li, Yi Li, Xiao-Fei Wang, Chuan-Kun Shan, Rui-Qi Wang, Yong-Su Zhen
Overexpression of EGFR and MMP-2 plays an essential role in the initiation and progression of non-small-cell lung carcinoma (NSCLC). In this study, a novel format of EGFR/MMP-2 bi-targeted fusion protein Ec-LDP-TIMP2 and its enediyne-integrated analogue Ec-LDP(AE)-TIMP2 have been prepared by genetic engineering and molecular reconstitution. The Ec-LDP(AE)-TIMP2 comprises endogenous inhibitor of matrix metalloproteinase 2 (TIMP2), EGF-derived oligopeptide (Ec), lidamycin apoprotein (LDP), and the extremely potent cytotoxic enediyne (AE)...
April 6, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/28392366/mirna-221-acts-as-an-oncogenic-role-by-directly-targeting-timp2-in-non-small-cell-lung-carcinoma
#14
Zijun Yin, Meng Xu, Ping Li
MicroRNA-221 (miRNA-221) plays an important role in occurrence and development of tumor. However, expression pattern and role of miRNA-221 in the non-small-cell lung carcinoma (NSCLC) remains poorly understood. In the current study, we explored the roles and the underlying mechanism of miR-221 in NSCLC by gain and loss of function analysis. We found that the expression of miRNA-221 is significantly higher in NSCLC tissues and NSCLC cells compared to normal tissues and cells, respectively (P<0.05). NSCLC cell lines SPCA1 and H1299 were transfected with miRNA-221 mimic or inhibitor, respectively, and then performed to analysis...
July 15, 2017: Gene
https://www.readbyqxmd.com/read/28382019/transcriptome-analysis-showed-a-differential-signature-between-invasive-and-non-invasive-corticotrophinomas
#15
Leonardo Jose Tadeu de Araújo, Antonio Marcondes Lerario, Margaret de Castro, Clarissa Silva Martins, Marcello Delano Bronstein, Marcio Carlos Machado, Ericka Barbosa Trarbach, Maria Candida Barisson Villares Fragoso
ACTH-dependent hypercortisolism caused by a pituitary adenoma [Cushing's disease (CD)] is the most common cause of endogenous Cushing's syndrome. CD is often associated with several morbidities, including hypertension, diabetes, osteoporosis/bone fractures, secondary infections, and increased cardiovascular mortality. While the majority (≈80%) of the corticotrophinomas visible on pituitary magnetic resonance imaging are microadenomas (MICs, <10 mm of diameter), some tumors are macroadenomas (MACs, ≥10 mm) with increased growth potential and invasiveness, exceptionally exhibiting malignant demeanor...
2017: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/28356942/anti-metastatic-activity-of-fangchinoline-in-human-gastric-cancer-ags-cells
#16
Zhengrong Chen, Tengfei He, Kui Zhao, Chungen Xing
Fangchinoline (FCL) is an active component isolated from the traditional medicinal plant Stephania tetrandra S. Moore, and has been reported to possess anti-cancer functions in several types of cancers; however, the effect of FCL on gastric cancer metastasis and its underlying molecular mechanisms remain unknown. The current study aimed to investigate the effect of FCL on the cell migration and invasion of human metastatic gastric cancer AGS cells and its mechanisms. Our study demonstrates that FCL dosage dependently suppressed the adhesion, migration and invasion capacities of human gastric cancer AGS cells without obvious cytotoxic effects...
February 2017: Oncology Letters
https://www.readbyqxmd.com/read/28353090/tissue-inhibitors-of-metalloproteinase-expressing-cells-in-human-anterior-pituitary-and-pituitary-adenoma
#17
Alimuddin Tofrizal, Ken Fujiwara, Morio Azuma, Motoshi Kikuchi, Depicha Jindatip, Takashi Yashiro, Shozo Yamada
Extracellular matrix (ECM) is essential in tissue physiology and pathologic conditions such as tumorigenesis. It affects tumor cell behavior, proliferation, and metastasis. Pituitary adenomas differ in their clinical characteristics, including ECM deposition, and we recently reported that the characteristics of collagen-producing cells differed between control human anterior pituitary gland and pituitary adenomas. ECM deposition is not defined solely by production; degradation and maintenance are also important...
March 28, 2017: Medical Molecular Morphology
https://www.readbyqxmd.com/read/28299490/molecular-effects-of-fractional-ablative-erbium-yag-laser-treatment-with-multiple-stacked-pulses-on-standardized-human-three-dimensional-organotypic-skin-models
#18
Laurenz Schmitt, P M Amann, Y Marquardt, R Heise, K Czaja, P A Gerber, T Steiner, F Hölzle, Jens Malte Baron
The molecular changes in gene expression following ablative laser treatment of skin lesions, such as atrophic scars and UV-damaged skin, are not completely understood. A standardized in vitro model of human skin, to study the effects of laser treatment on human skin, has been recently developed. Therefore, the aim of the investigation was to examine morphological and molecular changes caused by fractional ablative erbium:YAG laser treatment on an in vitro full-thickness 3D standardized organotypic model of human skin...
March 15, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28270187/multiplex-serum-protein-analysis-reveals-potential-mechanisms-and-markers-of-response-to-hyperimmune-caprine-serum-in-systemic-sclerosis
#19
Niamh Quillinan, Kristina E N Clark, Bryan Youl, Jeffrey Vernes, Deirdre McIntosh, Syed Haq, Christopher P Denton
BACKGROUND: Hyperimmune caprine serum (HICS) is a novel biological therapy with potential benefit for skin in established diffuse cutaneous systemic sclerosis. Here we report multiplex protein analysis of blood samples from a placebo-controlled phase II clinical trial and explore mechanisms of action and markers of response. METHODS: Patients were treated with HICS (n = 10) or placebo (n = 10) over 26 weeks, with follow-up open-label treatment to 52 weeks in 14 patients...
March 7, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28209269/association-of-matrix-metalloproteinase-levels-with-collagen-degradation-in-the-context-of-abdominal-aortic-aneurysm
#20
V Klaus, F Tanios-Schmies, C Reeps, M Trenner, E Matevossian, H-H Eckstein, J Pelisek
OBJECTIVE/BACKGROUND: Matrix metalloproteinases (MMPs) have already been identified as key players in the pathogenesis of abdominal aortic aneurysm (AAA). However, the current data remain inconclusive. In this study, the expression of MMPs at mRNA and protein levels were investigated in relation to the degradation of collagen I and collagen III. METHODS: Tissue samples were obtained from 40 patients with AAA undergoing open aortic repair, and from five healthy controls during kidney transplantation...
February 10, 2017: European Journal of Vascular and Endovascular Surgery
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