keyword
MENU ▼
Read by QxMD icon Read
search

TIMP2

keyword
https://www.readbyqxmd.com/read/28980922/rs3918242-variant-genotype-frequency-and-increased-timp-2-and-mmp-9-expression-are-positively-correlated-with-cancer-invasion-in-urinary-bladder-cancer
#1
S Pençe, E Özbek, N Ozan Tiryakioğlu, N Ersoy Tunali, H H Pençe, H Tunali
To study the role of MMP9 and TIMP2 genotypes and expression in predisposition to bladder cancer and relation with metastasis. 100 urinary bladder cancer patients and 100 healthy controls were included in the study. rs3918242 and rs8179090 genotypes were determined with PCR-RFLP. Quantitative real-time polymerase chain reaction was employed to assess the MMP-9 and TIMP-2 expression in tumors and adjacent healthy tissues. Variant genotype (TT) for rs3918242 polymorphism and rs8179090 variant genotype are not associated with bladder cancer risk...
September 30, 2017: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/28979918/dcas9-mediated-transcriptional-activation-of-tissue-inhibitor-of-metalloproteinases
#2
Tyler Duellman, Andrea Doll, Xi Chen, Rie Wakamiya, Jay Yang
Selective gene activation with the dCas9 (deactivated clustered regularly interspaced short palindromic repeats [CRISPR] associated protein 9)/CRISPR targeting of a transcriptional activator effector is now well established. However, the optimal targeting of guide RNA (gRNA) for a given gene is largely a matter of trial and error. We explored the optimal targeting site for tissue inhibitor of metalloproteinases (TIMPs) by first screening multiple gRNA target sites using a luciferase-based promoter-reporter system and next confirmed the effective TIMP induction in the mouse motor neuron-like neuron-enriched spinal cord 34 (NSC34) cells...
2017: Metalloproteinases in Medicine
https://www.readbyqxmd.com/read/28943392/an-integrative-analysis-of-chemically-induced-cirrhosis-associated-hepatocarcinogenesis-histological-biochemical-and-molecular-features
#3
Guilherme Ribeiro Romualdo, Tony Fernando Grassi, Renata Leme Goto, Mariana Baptista Tablas, Lucas Tadeu Bidinotto, Ana Angélica Henrique Fernandes, Bruno Cogliati, Luís Fernando Barbisan
This study aimed the integrative characterization of morphological, biochemical and molecular features of chemically-induced cirrhosis-associated hepatocarcinogenesis. Thus, male Wistar rats were submitted to a diethylnitrosamine (DEN)/thioacetamide (TAA)-induced model. Liver tissue was processed for global gene expression, histopathological and collagen evaluations; as well as immunohistochemical and oxidative stress analysis. Gene Ontology and functional analysis showed the upregulation of extracellular matrix deposition genes, such as collagen type I alpha 1 and 2 (Col1α1 and Col1α2) and tissue inhibitor of metalloproteinase 1 and 2 genes (Timp1 and Timp2)...
September 21, 2017: Toxicology Letters
https://www.readbyqxmd.com/read/28900109/region-specific-differential-corneal-and-scleral-mrna-expressions-of-mmp2-timp2-and-tgfb2-in-highly-myopic-astigmatic-chicks
#4
Lisa Yan-Yan Xi, Shea Ping Yip, Sze Wan Shan, Jody Summers-Rada, Chea-Su Kee
Myopia and astigmatism, two common refractive errors frequently co-exist, are affecting vision at all working distances in the affected populations worldwide. Eyeballs having these refractive errors are known to exhibit abnormal eye shape at the anterior and posterior eye segments, but whether the outer coats of these abnormal eyeballs, cornea anteriorly and sclera posteriorly, are regulated by region-specific molecular mechanism remains unclear. Here we presented the changes in mRNA expression levels of three genes (MMP2, TIMP2, and TGFB2), all known to participate in extracellular matrix organization, at five regions of the cornea and sclera in chickens developing high myopia and astigmatism induced by form deprivation...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28888475/promoter-methylation-status-of-the-timp2-and-adamts4-genes-and-patellar-tendinopathy
#5
Louis Y El Khoury, Rebecca Rickaby, Tom Samiric, Stuart M Raleigh
OBJECTIVES: Patellar tendinopathy (PT) is a debilitating and prevalent condition that tends to affect those who are physically active or engaged in jumping sports. Although tendinopathies are known to have a genetic basis, the role of DNA methylation as an epigenetic factor and risk determinant for human PT has never been described. We sought to determine whether differences existed between the methylation profiles of both the TIMP2 and ADAMTS4 gene promoter sequences in a cohort of males having undergone surgery for patellar tendinopathy compared to controls...
September 1, 2017: Journal of Science and Medicine in Sport
https://www.readbyqxmd.com/read/28871006/post-transcriptional-regulation-of-mmp16-and-timp2-expression-via-mir-382-mir-410-and-mir-200b-in-endometrial-cancer
#6
Beata Rak, Dawid Mehlich, Filip Garbicz, Zofia Domosud, Wiktor Paskal, Janina M Marczewska, Paweł K Włodarski
BACKGROUND/AIM: The post-transcriptional regulation of matrix metalloproteinases (MMPs) via microRNAs (miRNAs) has been recently described in numerous human malignancies. However, the exact mechanisms of miRNA-mediated MMPs deregulation in endometrial cancer (EC) remain unclear. Herein, we aimed to analyze the expression of MMP2, MMP16 and TIMP2 and identify miRNAs that modulate their expression. MATERIALS AND METHODS: Protein expression was assessed by immunohistochemistry in formalin-fixed paraffin-embedded EC samples...
September 2017: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/28843701/fine-particulate-matter-exposure-induces-cell-cycle-arrest-and-inhibits-migration-and-invasion-of-human-extravillous-trophoblast-as-determined-by-an-itraq-based-quantitative-proteomics-strategy
#7
Zhe Qin, Haiyan Hou, Feng Fu, Jun Wu, Bin Han, Wen Yang, Liwen Zhang, Jin Cao, Xiaohan Jin, Shixiang Cheng, Zhen Yang, Min Zhang, Xiaoxia Lan, Ting Yao, Qulong Dong, Siyu Wu, Jingjing Zhang, Zhongwei Xu, Yuming Li, Yaqiong Chen
Long-term exposure to fine particulate matter (PM2.5) may cause adverse pregnancy outcomes but the mechanisms are not clear. Our research confirms that PM2.5 induced DNA damage, and inhibited cell proliferation in HTR-8/SVneo cells, presenting in a dose- and time-dependent manners. Using quantitative proteomics, the 182 and 486 differentially expressed proteins in cells treated with 120μgml(-1) PM2.5 for 24 and 48h were involved in many critical biological processes, including of cell proliferation, response to DNA damage, regulation of small GTPase mediated signal transduction, and etc...
August 24, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/28807218/teduglutide-effects-on-gene-regulation-of-fibrogenesis-on-an-animal-model-of-intestinal-anastomosis
#8
Beatriz P Costa, Ana C Gonçalves, Ana M Abrantes, Paulo Matafome, Raquel Seiça, Ana B Sarmento-Ribeiro, Maria Filomena Botelho, Francisco Castro-Sousa
BACKGROUND: Teduglutide is an enterotrophic analog of glucagon-like peptide 2 approved for the rehabilitation of short-bowel syndrome. This study aims to analyze the effects of teduglutide administration on the gene regulation of fibrogenesis during the intestinal anastomotic healing on an animal model. METHODS: Wistar rats (n = 62) were assigned into four groups: "Ileal Resection and Anastomosis" or "Laparotomy," each one subdivided into "Postoperative Teduglutide Administration" or "No Treatment," and sacrificed at the third or at the seventh days, with ileal sample harvesting...
August 2017: Journal of Surgical Research
https://www.readbyqxmd.com/read/28802022/microrna-492-overexpression-involves-in-cell-proliferation-migration-and-radiotherapy-response-of-cervical-squamous-cell-carcinomas
#9
Mei Liu, Jusheng An, Manni Huang, Liming Wang, Binbin Tu, Yan Song, Kai Ma, Yu Wang, Shuren Wang, Hongxia Zhu, Ningzhi Xu, Lingying Wu
MicroRNAs (miRNAs) are small non-coding RNA that target protein-coding mRNAs at the post-transcriptional level. The aim of this study was to define the role of miR-492 in cervical squamous cell carcinomas. After microRNA profiling and comparison, we firstly detected miR-492 expression in 104 tumor tissues biopsies derived from advanced staged (FIGO IIB-IIIB) cervical squamous cell carcinoma patients before receiving concomitant chemoradiotherapy and found miR-492 expression was significantly higher in the specimens that were sensitive to concomitant chemoradiotherapy, as compared with insensitive cancer specimens (p < 0...
August 12, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28792670/comments-on-the-review-biomarkers-in-acute-kidney-injury-pathophysiological-basis-and-clinical-performance-acta-physiol-2017-219-556-574-an-update-on-kidney-localization-of-igfbp7-and-timp2
#10
David R Emlet, Xiaoyan Wen, John A Kellum
In March of 2017, Acta Physiologica published a special series on renoprotection, and one of the publications in that series was a review entitled "Biomarkers in acute kidney injury - pathophysiological basis and clinical performance". This review described very well the current understanding of the pathophysiology, cellular localization, and clinical performance of the most important currently proposed biomarkers of AKI, to include insulin-like growth factor binding protein 7 (IGFBP7) and tissue inhibitor of metalloproteinases 2 (TIMP2)...
August 9, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/28755073/urinary-biomarkers-of-cell-cycle-arrest-are-delayed-predictors-of-acute-kidney-injury-after-pediatric-cardiopulmonary-bypass
#11
Liqun Dong, Qing Ma, Michael Bennett, Prasad Devarajan
BACKGROUND: Several novel biomarkers that predict acute kidney injury (AKI) have recently been proposed. We have evaluated the sequential patterns of biomarker elevation after pediatric cardiopulmonary bypass (CPB) and determined their diagnostic accuracy. METHODS: We measured the ability of neutrophil gelatinase-associated lipocalin (NGAL), interleukin-18 (IL-18), liver type fatty-acid binding protein (L-FABP), kidney injury molecule-1 (KIM-1), tissue inhibitor of metalloproteinase-2 (TIMP-2), and insulin-like growth factor binding protein 7 (IGFBP7), to predict AKI (≥50% increase in serum creatinine from baseline)...
July 28, 2017: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/28742099/variants-of-acan-are-associated-with-severity-of-lumbar-disc-herniation-in-patients-with-chronic-low-back-pain
#12
Romain Shanil Perera, Poruwalage Harsha Dissanayake, Upul Senarath, Lalith Sirimevan Wijayaratne, Aranjan Lional Karunanayake, Vajira Harshadeva Weerabaddana Dissanayake
INTRODUCTION: Disc herniation is a complex spinal disorder associated with disability and high healthcare cost. Lumbar disc herniation is strongly associated with disc degeneration. Candidate genes of the aggrecan metabolic pathway may associate with the severity of lumbar disc herniation. OBJECTIVES: This study evaluated the association of single nucleotide variants (SNVs) of the candidate genes of the aggrecan metabolic pathway with the severity of lumbar disc herniation in patients with chronic mechanical low back pain...
2017: PloS One
https://www.readbyqxmd.com/read/28731196/microrna-106a-promotes-cell-migration-and-invasion-by-targeting-tissue-inhibitor-of-matrix-metalloproteinase-2-in-cervical-cancer
#13
Xia Li, Qi Zhou, Ling Tao, Chunxia Yu
Increasing evidence has demonstrated that miRNAs play a critical role in tumor development and progression. Previous studies have revealed that miR-106a is abnormally expressed in various cancers. However, its function and underlying mechanism in cervical cancer (CC) remains unknown. In this study, we confirmed that the expression of miR-106a was significantly upregulated in both CC cell lines and tissues by qRT-PCR. The increased expression of miR-106a was obviously associated with adverse prognostic features...
July 18, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28724216/the-extract-from-punica-granatum-pomegranate-peel-induces-apoptosis-and-impairs-metastasis-in-prostate-cancer-cells
#14
Yuanle Deng, Yali Li, Fangfang Yang, Anqi Zeng, Shuping Yang, Yi Luo, Yiwen Zhang, Yongmei Xie, Tinghong Ye, Yong Xia, Wenya Yin
Prostate cancer is a big threat to male for its poor prognosis and high mortality rate. Natural compounds are important resources of many anticancer drugs. Pomegranate is a kind of antioxidant-rich fruit and its peel and seed has potential anticancer activities. In this study, we aimed to investigate the effects of pomegranate peel extract (PoPx) on the apoptosis and metastasis of prostate cancer cells and the related mechanism. We found that PoPx showed growth inhibition on prostate cancer cells. Nuclei morphological and flow cytometer (FCM) analysis indicated that PoPx could induce prostate cancer apoptosis...
September 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28721084/urinary-timp2%C3%A2-igfbp7-for-the-prediction-of-platinum-induced-acute-renal-injury
#15
Moritz Schanz, Anette Hoferer, Jing Shi, Mark Dominik Alscher, Martin Kimmel
INTRODUCTION: Platinum-based chemotherapy (PBC) is a potent antineoplastic treatment, but cisplatin nephrotoxicity is often the limiting factor. Identifying the patients who are at risk for developing platinum-induced renal injury is an important issue. We tested urinary TIMP2·IGFBP7, a new US Food and Drug Administration (FDA)-cleared test to assess the risk of acute kidney injury (AKI), in a cohort of patients with malignant neoplastic disease receiving PBC. PATIENTS AND METHODS: A total of 58 patients with malignant neoplastic disease were enrolled in this study, of whom 32 patients had both urine samples and subsequent serum creatinine values available for detecting AKI within 72 hours...
2017: International Journal of Nephrology and Renovascular Disease
https://www.readbyqxmd.com/read/28625714/proteomic-analysis-of-seminal-plasma-from-locally-adapted-curraleiro-p%C3%A3-duro-bulls-bos-taurus-identifying-biomarkers-involved-in-sperm-physiology-in-endangered-animals-for-conservation-of-biodiversity
#16
E B Menezes, R V de Oliveira, M F van Tilburg, E A Barbosa, N V Nascimento, A L M C S Velho, F B Moreno, R A Moreira, A C O Monteiro-Moreira, G M C Carvalho, A F Ramos, E Memili, A A Moura
The present study was aimed at evaluating the seminal plasma proteins and sperm parameters of Curraleiro Pé-Duro bulls. Semen was collected from 10 bulls by electroejaculation, and sperm parameters were evaluated in fresh and frozen-thawed semen. Seminal plasma proteins were analyzed by 2-D SDS-PAGE and mass spectrophotometry. Tools in computational biology were used to generate bioinformatic knowledge and evaluate gene ontology, protein-protein interactions, phylogenetic trees and multiple sequence alignments...
May 29, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28620234/ezh2-mediated-epigenetic-silencing-of-timp2-promotes-ovarian-cancer-migration-and-invasion
#17
Xiaoqing Yi, Jianfeng Guo, Jing Guo, Si Sun, Ping Yang, Junjie Wang, Yuan Li, Lisha Xie, Jing Cai, Zehua Wang
Enhancer of zeste homolog 2 (EZH2) is often increased in malignant tumors and is involved in metastasis. EZH2 silences gene expression by tri-methylating the lysine 27 residue of histone H3 (H3K27me3). However, the mechanism underlying EZH2 promotion of ovarian cancer metastasis remains elusive. Here, we showed that EZH2 is up-regulated in ovarian cancer and is associated with tumor metastasis and poor survival by mRNA sequencing and microarray results from databases. Tissue microarray and immunohistochemistry results revealed that EZH2 was negatively correlated with the expression of tissue inhibitor of metalloproteinases 2 (TIMP2)...
June 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28570941/diet-dependent-retinoid-effects-on-liver-gene-expression-include-stellate-and-inflammation-markers-and-parallel-effects-of-the-nuclear-repressor-shp
#18
Meghan Maguire, Justin R Bushkofsky, Michele Campaigne Larsen, Yee Hoon Foong, Sherry A Tanumihardjo, Colin R Jefcoate
For mice, a maternal vitamin A (VA)-deficient diet initiated from midgestation (GVAD) produces serum retinol deficiency in mature offspring. We hypothesize that the effects of GVAD arise from preweaning developmental changes. We compare the effect of this GVAD protocol in combination with a postweaning high-fat diet (HFD) or high-carbohydrate diet (LF12). Each is compared to an equivalent VA-sufficient combination. GVAD extensively decreased serum retinol and liver retinol, retinyl esters, and retinoid homeostasis genes (Lrat, Cyp26b1 and Cyp26a1)...
September 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/28552795/rhukgf-ameliorates-protease-anti-protease-imbalance-in-emphysematous-mice
#19
Sudhir Kotnala, Amit Tyagi, Jai Prakash Muyal
We attempted to elucidate the beneficial role of rHuKGF supplementation in the amelioration of protease/antiprotease imbalance and TGF-β1 signaling pathway leading to alveolar tissue maintenance in elastase induced emphysematous mice. Thirty two male C57BL mice were divided into four groups i.e. control, emphysema, therapy and rHuKGF only and were oropharyngeally instilled with saline/porcine pancreatic elastase/rHuKGF. Subsequently, lungs from mice were collected for histopathology and molecular biology studies...
August 2017: Pulmonary Pharmacology & Therapeutics
https://www.readbyqxmd.com/read/28537976/interest-of-urinary-timp-2-%C3%A3-igfbp-7-for-predicting-the-occurrence-of-acute-kidney-injury-after-cardiac-surgery-a-gray-zone-approach
#20
Toufic Finge, Sébastien Bertran, Claire Roger, Damien Candela, Bruno Pereira, Christopher Scott, Laurent Muller, Benjamin Louart, Jean-Yves Lefrant
BACKGROUND: This study assessed the ability of 3-hour postoperative urinary tissue inhibitor of metalloproteinases-2 × insulin-like growth factor binding protein-7 ([TIMP-2] × [IGFBP-7]) to predict postoperative acute kidney injury (AKI) in patients undergoing cardiopulmonary bypass during cardiac surgery. METHODS: Patients undergoing cardiac surgery with cardiopulmonary bypass were eligible for this study. Patients with initial chronic renal insufficiency requiring renal replacement therapy, patients <18 years of age, and parturients were not included...
September 2017: Anesthesia and Analgesia
keyword
keyword
49883
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"