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https://www.readbyqxmd.com/read/29245117/gold-nanoflowers-synthesized-using-acanthopanacis-cortex-extract-inhibit-inflammatory-mediators-in-lps-induced-raw264-7-macrophages-via-nf-%C3%AE%C2%BAb-and-ap-1-pathways
#1
Sungeun Ahn, Priyanka Singh, Mi Jang, Yu-Jin Kim, Verónica Castro-Aceituno, Shakina Yesmin Simu, Yeon Ju Kim, Deok-Chun Yang
We reported the rapid synthesis (<8s) of gold nanoparticles at room temperature using Acanthopanacis cortex extract (A-AuNPs). We characterized the A-AuNPs using several analytical techniques and found that nano-flower type A-AuNPs, which are known to possess a coarse surface with a high surface to volume ratio, conferring these particles with high binding capacity for various biological molecules. After confirming the stability of the nanoparticles, we investigated the anti-inflammatory effect of A-AuNPs in LPS-stimulated RAW264...
November 17, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29245067/lin28a-functionally-modulates-bupivacaine-induced-dorsal-root-ganglion-neuron-apoptosis-through-trka-activation
#2
Liangsheng Yu, Yuanxu Jiang, Bin Tang
PURPOSE: Lin-28 Homolog A gene (Lin28a) is an important regulator in nerve system. In this study, we investigated the functional mechanism of Lin28a during the process of bupivacaine (BUP)-induced neuronal apoptosis of spinal cord dorsal root ganglion neurons (DRGNs). METHODS: Young mouse DRGNs were cultured in vitro and treated with series concentrations of BUP. Apoptosis was evaluated by TUNEL assay. Corresponding Lin28a mRNA and protein expressions were evaluated by qRT-PCR and western blot (WB) assays...
December 12, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29245047/polyriboinosinic-polyribocytidylic-acid-facilitates-interleukin-6-and-interleukin-8-secretion-in-human-dermal-fibroblasts-via-the-jak-stat3-and-p38-mapk-signal-transduction-pathways
#3
Zhang Ying Zhu, Cong Zhuo Jia, Jian Min Luo, Li Wang
Polyriboinosinic-polyribocytidylic acid (polyI:C) is a viral dsRNA analoguethat promotes wounds healing, accelerates re-epithelialization, granulation and neovascularization, and induces pro-inflammatory cytokine release. Little is known about polyI:C mediated induction of inflammatory mediators in human dermal fibroblast (HDFs), which form the primary scaffold for epithelial cells covering the wound. Here, we found that polyI:C enhances IL-6 and IL-8 mRNA expression and induces of IL-6 and IL-8 production in a concentration-dependent and time-dependent manner in HDFs...
December 12, 2017: Cytokine
https://www.readbyqxmd.com/read/29245030/osmotin-a-plant-defense-tool-against-biotic-and-abiotic-stresses
#4
REVIEW
Hakim, Abid Ullah, Amjad Hussain, Muhammad Shaban, Aamir Hamid Khan, Muna Alariqi, Summia Gul, Zhang Jun, Sun Lin, Jianying Li, Shuangxia Jin, Muhammad Farooq Hussain Munis
Plants are prone to a number of pathogens and abiotic stresses that cause various disorders. However, plants possess a defense mechanism to cope with these stresses. The osmotin protein belongs to the PR-5 family of Pathogenesis-related (PR) proteins, which are produced in response to diseases caused by various biotic and abiotic stresses. Osmotin uses a signal transduction pathway to inhibit the activity of defensive cell wall barriers and increases its own cytotoxic efficiency. However, in response to cytotoxic effects, this pathway stimulates a mitogen-activated protein kinase (MAPK) cascade that triggers changes in the cell wall and enables osmotin's entrance into the plasma membrane...
December 7, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29244975/review-of-the-molecular-mechanisms-in-wound-healing-new-therapeutic-targets
#5
B Palmieri, M Vadalà, C Laurino
The restoration of the skin barrier in acute and chronic wounds is controlled by several molecular mechanisms that synergistically regulate cell kinetics, enzymatic functions, and neurovascular activation. These pathways include genetic and epigenetic activation, which modulate physiological wound healing. Our review describes the genetic background of skin repair, namely transcription-independent diffusible damage signals, individual variability, epigenetic mechanism, controlled qualitative traits, post-translational mechanisms, antioxidants, nutrients, DNA modifications, bacteria activation, mitochondrial activity, and oxidative stress...
December 2, 2017: Journal of Wound Care
https://www.readbyqxmd.com/read/29244903/dermal-fibroblast-in-cutaneous-development-and-healing
#6
REVIEW
Venkata Thulabandu, Demeng Chen, Radhika P Atit
The skin is the largest organ of the body and is composed of two layers: the overlying epidermis and the underlying dermis. The dermal fibroblasts originate from distinct locations of the embryo and contain the positional identity and patterning information in the skin. The dermal fibroblast progenitors differentiate into various cell types that are fated to perform specific functions such as hair follicle initiation and scar formation during wound healing. Recent studies have revealed the heterogeneity and plasticity of dermal fibroblasts within skin, which has implications for skin disease and tissue engineering...
December 15, 2017: Wiley Interdisciplinary Reviews. Developmental Biology
https://www.readbyqxmd.com/read/29244872/the-ubiquitin-proteasome-system-is-required-for-african-swine-fever-replication
#7
Lucía Barrado-Gil, Inmaculada Galindo, Diego Martínez-Alonso, Sergio Viedma, Covadonga Alonso
Several viruses manipulate the ubiquitin-proteasome system (UPS) to initiate a productive infection. Determined viral proteins are able to change the host's ubiquitin machinery and some viruses even encode their own ubiquitinating or deubiquitinating enzymes. African swine fever virus (ASFV) encodes a gene homologous to the E2 ubiquitin conjugating (UBC) enzyme. The viral ubiquitin-conjugating enzyme (UBCv1) is expressed throughout ASFV infection and accumulates at late times post infection. UBCv is also present in the viral particle suggesting that the ubiquitin-proteasome pathway could play an important role at early ASFV infection...
2017: PloS One
https://www.readbyqxmd.com/read/29244796/rsv-bronchiolitis-versus-rhinovirus-difference-in-nasal-airway-microrna-profiles-and-nf%C3%AE%C2%BAb-signaling
#8
Kohei Hasegawa, Marcos Pérez-Losada, Claire E Hoptay, Samuel Epstein, Jonathan M Mansbach, Stephen J Teach, Pedro A Piedra, Carlos A Camargo, Robert J Freishtat
BACKGROUND: Although rhinovirus infection is associated with increased risks of acute and chronic respiratory outcomes during childhood compared with respiratory syncytial virus (RSV), the underlying mechanisms remain unclear. We aimed to determine differences in nasal airway microRNA profiles and their downstream effects between infants with rhinovirus and RSV bronchiolitis. METHODS: As part of multicenter cohort study of infants hospitalized for bronchiolitis, we examined nasal samples obtained from 16 infants with rhinovirus and 16 infants with RSV...
December 15, 2017: Pediatric Research
https://www.readbyqxmd.com/read/29244790/lentivirus-mediated-silencing-of-the-ptc1-and-ptc2-genes-promotes-recovery-from-spinal-cord-injury-by-activating-the-hedgehog-signaling-pathway-in-a-rat-model
#9
Ya-Dong Zhang, Zhong-Sheng Zhu, Dong Zhang, Zhen Zhang, Bin Ma, Shi-Chang Zhao, Feng Xue
This study aimed to investigate the effect of Patched-1 (PTC1) and PTC2 silencing in a rat model, on Hedgehog (Hh) pathway-mediated recovery from spinal cord injury (SCI). An analytical emphasis on the relationship between the sonic hedgehog (Shh) pathway and nerve regeneration was explored. A total of 126 rats were divided into normal, sham, SCI, negative control (NC), PTC1-RNAi, PTC2-RNAi and PTC1/PTC2-RNAi groups. The Basso, Beattie and Bresnahan (BBB) scale was employed to assess hind limb motor function...
December 15, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29244788/dual-modulation-of-human-hepatic-zonation-via-canonical-and-non-canonical-wnt-pathways
#10
Laura McEnerney, Kara Duncan, Bo-Ram Bang, Sandra Elmasry, Meng Li, Toshio Miki, Sadeesh K Ramakrishnan, Yatrik M Shah, Takeshi Saito
The hepatic lobule is divided into three zones along the portal-central vein axis. Hepatocytes within each zone exhibit a distinctive gene expression profile that coordinates their metabolic compartmentalization. The zone-dependent heterogeneity of hepatocytes has been hypothesized to result from the differential degree of exposure to oxygen, nutrition and gut-derived toxins. In addition, the gradient of Wnt signaling that increases towards the central vein seen in rodent models is believed to play a critical role in shaping zonation...
December 15, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29244752/proteomic-analysis-reveals-coordinated-regulation-of-anthocyanin-biosynthesis-through-signal-transduction-and-sugar-metabolism-in-black-rice-leaf
#11
Linghua Chen, Yining Huang, Ming Xu, Zuxin Cheng, Jingui Zheng
Black rice (Oryza sativa L.) is considered to be a healthy food due to its high content of anthocyanins in the pericarp. The synthetic pathway of anthocyanins in black rice grains has been identified, however, the proteomic profile of leaves during grain development is still unclear. Here, isobaric Tags Relative and Absolute Quantification (iTRAQ) MS/MS was carried out to identify statistically significant changes of leaf proteome in the black rice during grain development. Throughout three sequential developmental stages, a total of 3562 proteins were detected and 24 functional proteins were differentially expressed 3-10 days after flowering (DAF)...
December 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29244196/iron-overload-induces-g1-phase-arrest-and-autophagy-in-murine-preosteoblast-cells
#12
Wan-Jing Cen, Yi Feng, Shu-Shu Li, Wei-Liang Huang, Tao Zhang, Wu Zhang, Wei-Dong Kong, Jian-Wei Jiang
This study aimed to investigate the cell cycle arrest and autophagy induced by iron overload inMC3T3-E1 cells. MC3T3-E1 cells were cultured in different concentrations of ferric ammonium citrate (FAC), and Perls' Prussian blue reaction was used to detect the iron levels of the cells. CCK-8 assays were used to detect the growth of MC3T3-E1. The level of reactive oxygen species (ROS) within cells was investigated with DCFH-DA. PI staining was used to analyze the cell cycle distribution of MC3T3-E1 cells. Finally, the expression levels of cell cycle related proteins, autophagy related proteins, AKT, p38 MAPK, Stat3 and their downstream proteins were detected with western blot assays...
December 15, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29244193/comparative-peptidomic-profile-between-human-hypertrophic-scar-tissue-and-matched-normal-skin-for-identification-of-endogenous-peptides-involved-in-scar-pathology
#13
Jingyun Li, Ling Chen, Qian Li, Jing Cao, Yanli Gao, Jun Li
Endogenous peptides recently attract increasing attention for their participation in various biological processes. Their roles in the pathogenesis of human hypertrophic scar remains poorly understood. In this study, we used liquid chromatography-tandem mass spectrometry to construct a comparative peptidomic profiling between human hypertrophic scar tissue and matched normal skin. A total of 179 peptides were significantly differentially expressed in human hypertrophic scar tissue, with 95 upregulated and 84 downregulated peptides between hypertrophic scar tissue and matched normal skin...
December 15, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29244152/regulation-of-t-cell-proliferation-with-drug-responsive-microrna-switches
#14
Remus S Wong, Yvonne Y Chen, Christina D Smolke
As molecular and cellular therapies advance in the clinic, the role of genetic regulation is becoming increasingly important for controlling therapeutic potency and safety. The emerging field of mammalian synthetic biology provides promising tools for the construction of regulatory platforms that can intervene with endogenous pathways and control cell behavior. Recent work has highlighted the development of synthetic biological systems that integrate sensing of molecular signals to regulated therapeutic function in various disease settings...
December 13, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29243967/extracts-of-porphyra-tenera-nori-seaweed-activate-the-immune-response-in-mouse-raw264-7-macrophages-via-nf-%C3%AE%C2%BAb-signaling
#15
Ji-Hye Song, Hee-Bum Kang, Seung-Ho Park, Ji-Hoon Jeong, Jeongjin Park, Yanghee You, Yoo-Hyun Lee, Jeongmin Lee, Eungpil Kim, Kyung-Chul Choi, Woojin Jun
Porphyra tenera, also known as nori, is a red algal species of seaweed. It is cultivated in Asia for culinary purposes. We report that P. tenera extract (PTE) enhances the immune response in mouse macrophages. We found that P. tenera extract regulates the NF-κB IκB kinase (IKK) signaling pathway, and we assessed the expression and translocation of p65, a subunit of NF-κB, in RAW264.7 mouse macrophage cells after treatment with PTE. We also investigated the effects of 10% ethanol PTE (PTE10) in RAW264.7 cells...
December 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/29243844/in-vitro-improvement-of-quail-primordial-germ-cell-expansion-through-activation-of-tgf-beta-signaling-pathway
#16
Saeed Yakhkeshi, Shaban Rahimi, Mohsen Sharafi, Seyedeh-Nafiseh Hassani, Sara Taleahmad, Abdolhossein Shahverdi, Hossein Baharvand
Avian primordial germ cells (PGCs) have valuable potentials to cell-based approaches for transgenic bird production. In this regard, improvement of avian PGC expansion in vitro is necessary. Among experimental avian species, quail is a good model for transgenic technology, especially due to its short generation time. In the present study, we have examined the proliferative effects of transforming growth factor β (TGF-β) on the quail PGCs. After isolation of quail PGCs from blood [Hamburger-Hamilton (HH stages 13-15)] and gonads (HH stages 28-30), these cells were cultured on quail embryonic fibroblasts (QEF)...
December 15, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29243843/signaling-cascades-in-thyroid-cancer-increasing-the-armory-of-archers-to-hit-bullseye
#17
Faiza Abdul Rashid, Qaisar Mansoor, Hafsa Aziz, Sobia Tabassum, Waleed O Arfat, George E Naoum, Muhammad Ismail, Ammad Ahmad Farooqi
Thyroid cancer is a multifaceted and therapeutically challenging disease and rapidly accumulating experimentally verified findings have considerably improve our understanding of the molecular mechanisms which underlie its development. Substantial fraction of information has been added into existing landscape of molecular oncology and we have started to develop a sharper understanding of the underlying mechanisms of thyroid cancer. Wealth of information demystified different intracellular signaling cascades which are frequently deregulated in thyroid cancer...
December 15, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29243835/diallyl-trisulfide-inhibits-breast-cancer-stem-cells-via-suppression-of-wnt-%C3%AE-catenin-pathway
#18
Xiaoting Li, Yu Meng, Chunfeng Xie, Jianyun Zhu, Xiaoqian Wang, Yuan Li, Shanshan Geng, Jieshu Wu, Caiyun Zhong, Min Li
Cancer stem cells (CSCs) play a central role in the development of breast cancer. The canonical Wnt/β-catenin signal pathway is critical for maintaining CSCs characteristics. Diallyl trisulfide (DATS), a natural organosulfur compound from the garlic, exhibits effective antitumor properties. However, the role of DATS in regulating breast CSCs activity and the underlying molecular mechanisms remain obscure. In the present study, we reported that DATS efficiently inhibited the viability of breast CSCs as evidenced by reducing turmorspheres formation, decreasing the expression of breast CSCs markers (CD44, ALDH1A1, Nanog and Oct4), as well as inhibiting proliferation and inducing apoptosis...
December 15, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29243812/dysregulated-mitogen-activated-protein-kinase-signalling-as-an-oncogenic-basis-for-clear-cell-sarcoma-of-the-kidney
#19
Colin Kenny, Naomi McDonagh, Antonio Lazaro, Elaine O'Meara, Rut Klinger, Darran O'Connor, Fiona Roche, Karsten Hokamp, Maureen J O'Sullivan
The oncogenic mechanisms and tumour biology underpinning Clear Cell Sarcoma of Kidney (CCSK), the second commonest paediatric renal malignancy, are poorly understood and currently therapy depends heavily on doxorubicin with cardiotoxic side-effects. Previously, we characterised the balanced t(10;17)(q22;p13) chromosomal translocation, identified at that time as the only recurrent genetic aberration in CCSK. This translocation results in an in-frame fusion of the genes YWHAE (encoding 14-3-3ε)and NUTM2, with a somatic incidence of 12%...
December 15, 2017: Journal of Pathology
https://www.readbyqxmd.com/read/29243780/long-non-coding-rna-crnde-is-a-novel-tumor-promoter-by-modulating-pi3k-akt-signal-pathways-in-human-gastric-cancer
#20
D-X Du, D-B Lian, B-H Amin, W Yan
OBJECTIVE: Long non-coding RNA CRNDE (CRNDE) recently emerged as a carcinogenic promoter in various cancers including gastric cancer (GC). However, the functions and molecular mechanisms of CRNDE to GC are still largely unclear. The aim of this study was to investigate the clinical significance and functional mechanisms of CRNDE expression in GC. PATIENTS AND METHODS: The expression of CRNDE was detected by quantitative Real-time PCR (qRT-PCR) in GC specimens and cell lines...
December 2017: European Review for Medical and Pharmacological Sciences
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