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https://www.readbyqxmd.com/read/29909551/combined-use-of-leptin-and-mechanical-stress-has-osteogenic-effects-on-ossification-of-the-posterior-longitudinal-ligament
#1
Shuai Chen, Haifeng Zhu, Gangliang Wang, Ziang Xie, Jiying Wang, Jian Chen
PURPOSE: To evaluate the effects of leptin/leptin receptor (LepR) combined with mechanical stress on the development of ossification of the posterior longitudinal ligament (OPLL), which is a disease characterized by ectopic bone formation of the posterior longitudinal ligament (PLL) and can lead to radiculopathy and myelopathy. METHODS: Six human samples of the PLL were analyzed for the expression of leptin and LepR by RT-PCR and western blotting. PLL cells were stimulated with leptin and mechanical stress delivered via a Flexcell tension system, and osteogenic differentiation was evaluated by RT-PCR and western blotting analysis of osteogenic marker expression as well as by alkaline phosphatase (ALP) staining and alizarin red S staining...
June 16, 2018: European Spine Journal
https://www.readbyqxmd.com/read/29908894/microrna-499a-decelerates-glioma-cell-proliferation-while-accelerating-apoptosis-through-the-suppression-of-notch1-and-the-mapk-signaling-pathway
#2
Bang-Qing Wang, Bin Yang, Hua-Chao Yang, Jun-Yi Wang, Sen Hu, Yu-Shuai Gao, Xing-Yao Bu
As the most common and lethal of intracranial tumors, glioma accounts for 81% of all malignant brain tumors. Research data has identified the role of microRNAs (miRs) as functional suppressers in the progression of Glioma. The present study aimed to, ascertain as to whether microRNA-499a (miR-499a) influences cell proliferation and apoptosis through the MAPK signaling pathway by targeting Notch1 in glioma. Both glioma and adjacent tissues between 2012~2016, were obtained from People's Hospital of Zhengzhou University (Henan Provincial People's Hospital)...
June 14, 2018: Brain Research Bulletin
https://www.readbyqxmd.com/read/29908638/gadd45a-knockdown-alleviates-oxidative-stress-through-suppressing-the-p38-mapk-signaling-pathway-in-the-pathogenesis-of-preeclampsia
#3
Fa-Hong Li, Na Han, Yong Wang, Qian Xu
OBJECTIVE: Preeclampsia (PE) is a hypertensive disease associated with vascular oxidative stress (OS). Besides, cell response to OS triggers growth arrest and DNA damage inducible alpha (Gadd45a). Herein, we investigated the effect of Gadd45a on OS in PE. METHODS: Umbilical cord tissues and peripheral blood samples were collected from PE patients and normal pregnant women. Immunohistochemistry was applied to identify Gadd45a level and extent of p38 phosphorylation...
May 2018: Placenta
https://www.readbyqxmd.com/read/29907213/carbon-monoxide-attenuates-lipopolysaccharide-induced-lung-injury-by-mitofusin-proteins-via-p38-mapk-pathway
#4
Shu-An Dong, Yuan Zhang, Jian-Bo Yu, Xiang-Yun Li, Li-Rong Gong, Jia Shi, Yuan-Yuan Kang
BACKGROUND: The protective effects of carbon monoxide against the lipopolysaccharide (LPS)-induced lung injury were attributed to maintenance of mitochondrial dynamics, but the mechanisms remain unexplored. MATERIALS AND METHODS: Using a rat model of acute lung injury induced by LPS and the LPS attacking cell model, we investigated the effects of pretreatment of carbon monoxide molecule-2 (CORM-2) on the acute lung injury and expressions of mitofusin proteins that play a critical role in mitochondrial dynamics...
August 2018: Journal of Surgical Research
https://www.readbyqxmd.com/read/29906814/magnoflorine-enhances-lps-activated-pro-inflammatory-responses-via-myd88-dependent-pathways-in-u937-macrophages
#5
Md Areeful Haque, Ibrahim Jantan, Hemavathy Harikrishnan, Siti Mariam Abdul Wahab
Magnoflorine, a major bioactive metabolite isolated from Tinospora crispa, has been reported for its diverse biochemical and pharmacological properties. However, there is little report on its underlying mechanisms of action on immune responses, particularly on macrophage activation. In this study, we aimed to investigate the effects of magnoflorine, isolated from T. crispa on the pro-inflammatory mediators generation induced by LPS and the concomitant NF- κ B, MAPKs, and PI3K-Akt signaling pathways in U937 macrophages...
June 15, 2018: Planta Medica
https://www.readbyqxmd.com/read/29906743/costunolide-ameliorates-lipoteichoic-acid-induced-acute-lung-injury-via-attenuating-mapk-signaling-pathway
#6
Zhengxu Chen, Dan Zhang, Man Li, Baolong Wang
Lipoteichoic acid (LTA)-induced acute lung injury (ALI) is an experimental model for mimicking Gram-positive bacteria-induced pneumonia that is a refractory disease with lack of effective medicines. Here, we reported that costunolide, a sesquiterpene lactone, ameliorated LTA-induced ALI. Costunolide treatment reduced LTA-induced neutrophil lung infiltration, cytokine and chemokine production (TNF-α, IL-6 and KC), and pulmonary edema. In response to LTA challenge, treatment with costunolide resulted less iNOS expression and produced less inflammatory cytokines in bone marrow derived macrophages (BMDMs)...
June 12, 2018: International Immunopharmacology
https://www.readbyqxmd.com/read/29906250/oxidant-sensor-cation-channel-trpm2-regulates-neutrophil-extracellular-trap-formation-and-protects-against-pneumoseptic-bacterial-infection
#7
Jitendra Kumar Tripathi, Atul Sharma, Pramod Sukumaran, Yuyang Sun, Bibhuti Bhusan Mishra, Brij Bhan Singh, Jyotika Sharma
Neutrophil extracellular trap (NET) formation constitutes an important extracellular antimicrobial function of neutrophils that plays a protective role in bacterial pneumonia. Formation of reactive oxygen species (ROS) such as highly diffusible hydrogen peroxide (H2 O2 ) is a hallmark of oxidative stress during inflammatory lung conditions including pneumonia. However, the impact of exogenous ROS on NET formation and the signaling pathway involved in the process is not completely understood. Here we demonstrate that the ROS-sensing, nonselective, calcium-permeable channel transient receptor potential melastatin 2 (TRPM2) is required for NET formation in response to exogenous H2 O2 ...
June 15, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29904922/sideroxylin-callistemon-lanceolatus-suppressed-cell-proliferation-and-increased-apoptosis-in-ovarian-cancer-cells-accompanied-by-mitochondrial-dysfunction-the-generation-of-reactive-oxygen-species-and-an-increase-of-lipid-peroxidation
#8
Sunwoo Park, Whasun Lim, Wonsik Jeong, Fuller W Bazer, Dongho Lee, Gwonhwa Song
Sideroxylin is a C-methylated flavone isolated from Callistemon lanceolatus and exerts antimicrobial activity against Staphylococcus aureus. However, the anticancer effects of sideroxylin and its intracellular signaling mechanisms have not yet been identified. Results of our study showed that sideroxylin decreased cell proliferation and increased apoptosis, causing DNA fragmentation, depolarization of the mitochondrial membrane, the generation of reactive oxygen species, and an increase of lipid peroxidation in ovarian cancer cells (ES2 and OV90 cells)...
June 15, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29904407/the-cardioprotective-effects-of-secretory-leukocyte-protease-inhibitor-against-myocardial-ischemia-reperfusion-injury
#9
Eakkapote Prompunt, Jantira Sanit, Stephanie Barrère-Lemaire, Joel Nargeot, Hannah Noordali, Melanie Madhani, Sarawut Kumphune
Protease enzymes generated from injured cells and leukocytes are the primary cause of myocardial cell damage following ischemia/reperfusion (I/R). The inhibition of protease enzyme activity via the administration of particular drugs may reduce injury and potentially save patients' lives. The aim of the current study was to investigate the cardioprotective effects of treatment with recombinant human secretory leukocyte protease inhibitor (rhSLPI) on in vitro and ex vivo models of myocardial I/R injury. rhSLPI was applied to isolated adult rat ventricular myocytes (ARVMs) subjected to simulated I/R and to ex vivo murine hearts prior to I/R injury...
June 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29904339/neuroprotective-effects-of-proanthocyanidins-natural-flavonoids-derived-from-plants-on-rotenone-induced-oxidative-stress-and-apoptotic-cell-death-in-human-neuroblastoma-sh-sy5y-cells
#10
Jian Ma, Shan-Shan Gao, Hai-Jie Yang, Mian Wang, Bin-Feng Cheng, Zhi-Wei Feng, Lei Wang
Proanthocyanidins (PA) are natural flavonoids widely present in many vegetables, fruits, nuts and seeds, and especially in grape seed. In the present study, we examined the neuroprotective effects of PA and the underlying molecular mechanism in rotenone model of Parkinson's disease (PD). We found that pretreatment with PA significantly reduced rotenone-induced oxidative stress in human neuroblastoma SH-SY5Y dopaminergic cells. In addition, PA markedly enhanced cell viability against rotenone neurotoxicity and considerably blocked rotenone-induced activation of caspase-9, caspase-3, and cleavage of poly (ADP-ribose) polymerase (PARP), biochemical features of apoptosis...
2018: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/29904032/phloretin-promotes-adipogenesis-via-mitogen-activated-protein-kinase-pathways-in-mouse-marrow-stromal-st2-cells
#11
Ayumu Takeno, Ippei Kanazawa, Masakazu Notsu, Ken-Ichiro Tanaka, Toshitsugu Sugimoto
Phloretin, a glucose transporter (GLUT) inhibitor, has pleiotropic effects. The present study examined the effects of phloretin on the commitment of marrow stromal cells to adipocytes, using the mouse marrow stromal cell line ST2. Oil red O staining showed that treatment with phloretin 10⁻100 µM promoted lipid accumulation. Real-time PCR showed that phloretin significantly increased the expression of adipogenic markers, including PPARγ, C/EBPα, fatty acid synthase, fatty acid-binding protein 4, and adiponectin...
June 14, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29904013/farrerol-relieve-lipopolysaccharide-lps-induced-mastitis-by-inhibiting-akt-nf-%C3%AE%C2%BAb-p65-erk1-2-and-p38-signaling-pathway
#12
Yanwei Li, Qian Gong, Wenjin Guo, Xingchi Kan, Dianwen Xu, He Ma, Shoupeng Fu, Juxiong Liu
Farrerol has been proved to have an anti-inflammatory effect. However, the effects of farrerol on mastitis have not been investigated. This study was aimed to investigate the effect and mechanism of farrerol in lipopolysaccharide (LPS)-induced mouse mastitis and LPS-induced inflammatory response of mouse mammary epithelial cells (mMECs). In vivo, LPS were injected to the tetrad pair of nipples for establishing mouse mastitis, and then tested the effect of farrerol on histopathological changes, inflammatory response and activation degree of protein kinase B (AKT), nuclear factor-kappa B p65 (NF-κB p65), p38, extracellular regulated protein kinase (ERK1/2)...
June 14, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29903983/4-methoxyresveratrol-alleviated-age-induced-inflammation-via-rage-mediated-nf-%C3%AE%C2%BAb-and-nlrp3-inflammasome-pathway
#13
Wenzhe Yu, Mengru Tao, Yueliang Zhao, Xiaoqian Hu, Mingfu Wang
Advanced glycation end products (AGEs) could interact with the receptor for AGE (RAGE) as a sterile danger signal to induce inflammation. 4′-methoxyresveratrol (4′MR), a polyphenol derived from Dipterocarpaceae, has not been studied for its anti-inflammation effects. In the present study, we sought to explore the protective role of 4′MR in AGEs-induced inflammatory model using RAW264.7 macrophages. 4′MR significantly inhibited gene expression of pro-inflammatory cytokines and chemokines, such as interleukin 1β (IL-1β), interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-α) and monocyte chemoattractant protein-1 (MCP-1), as well as two typical pro-inflammatory enzymes, inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX2)...
June 14, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29903766/cytoprotective-and-pro-angiogenic-functions-of-thrombomodulin-are-preserved-in-the-c-loop-of-the-fifth-epidermal-growth-factor-like-domain
#14
Xiangmin Wang, Bin Pan, Goichi Honda, Xintao Wang, Yuko Hashimoto, Hiroshi Ohkawara, Kailin Xu, Lingyu Zeng, Takayuki Ikezoe
We previously found that the fifth epidermal growth factor-like domain of thrombomodulin (TME5) exerts cytoprotective and pro-angiogenic functions via G-protein coupled receptor 15 (GPR15). TME5 is comprised of three S-S bonds that divide it into three loops: A (TME5A), B (TME5B), and C (TME5C). Here, we identified the minimum structure of TME5 that produces favorable effects in vascular endothelial cells (ECs). We found that TME5C, composed of 19 amino acids, but not TME5A or TME5B, stimulated the proliferation of human umbilical vein endothelial cells (HUVECs) and human hepatic sinusoidal endothelial cells (HHSECs)...
June 14, 2018: Haematologica
https://www.readbyqxmd.com/read/29903060/bifunctional-supramolecular-hydrogel-alleviates-myocardial-ischemia-reperfusion-injury-by-inhibiting-autophagy-and-apoptosis
#15
Yi Deng, Guoqin Chen, Min Ye, Yingyan He, Zehua Li, Xianbao Wang, Caiwen Ou, Zhimou Yang, Minsheng Chen
Generally, nitric oxide (NO) is an important multi-functional cardioprotective soluble gas molecule. However, it may be detrimental when in excessive levels or combined with O2- a kind of reactive oxygen species (ROS)-to form ONOO-. The latter will rapidly decompose to highly reactive oxidant components. ROS will be abundantly produced during the ischemia/reperfusion (I/R) procedure. Therefore, an NO donor coupled with another antioxidant would be a more promising strategy for I/R treatment. In this study, we report on a novel self-assembly supramolecular hydrogel capable of constantly releasing both NO and curcumin (Cur) simultaneously, and we found that the combinational treatment of Cur and NO from the gel could efficiently reduce I/R injuries...
August 1, 2018: Journal of Biomedical Nanotechnology
https://www.readbyqxmd.com/read/29902518/differential-effects-of-typical-and-atypical-antipsychotics-on-astroglial-cells-in-vitro
#16
Larissa Daniele Bobermin, Amanda da Silva, Diogo Onofre Souza, André Quincozes-Santos
Astrocytes are glial cells that are essential for the maintenance of central nervous system functions, modulating neurotransmitters, providing metabolic, trophic and antioxidant support, and producing a wide range of cytokines to modulate the inflammatory response. These cells can be targets for antipsychotics, medications used in the treatment of neuropsychiatric disorders. In this regard, several studies have shown that antipsychotics are able to modulate peripheral cytokine release, but their effects on astroglial inflammatory response need to be further investigated...
June 11, 2018: International Journal of Developmental Neuroscience
https://www.readbyqxmd.com/read/29901206/protein-kinase-d1-regulates-hypoxic-metabolism-through-hif-1%C3%AE-and-glycolytic-enzymes-incancer-cells
#17
Jiao Chen, Bomiao Cui, Yaping Fan, Xiaoying Li, Qian Li, Yue Du, Yun Feng, Ping Zhang
Protein kinase D1 (PKD1), one of the protein kinase D (PKD) family members, plays a prominent role in multiple bio-behaviors of cancer cells. Low pH and hypoxia are unique characteristics of the tumor microenvironment. The aim of this study was to investigate the role and mechanism of PKD1 in regulating metabolism in the human tongue squamous cell carcinoma (TSCC) cell line SCC25 under a hypoxic condition, as well as growth and apoptosis. Here, we found that hypoxia not only induced the expression of HIF-1α, but also induced the expression and activation of PKD1...
June 7, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29901190/curcumin-suppresses-cardiac-fibroblasts-activities-by-regulating-the-proliferation-and-cell-cycle-via-the-inhibition-of-the-p38-mapk-erk-signaling-pathway
#18
Guanhua Fang, Shaoqin Chen, Qiuyu Huang, Liangwan Chen, Dongshan Liao
Cardiac fibrosis is a deleterious effect of many cardiovascular diseases. Previous studies have shown that curcumin has exhibited protective effects on cardiovascular diseases. The aim of the present study was to evaluate the effects of curcumin on the activity of human cardiac fibroblasts (CFs) and to elucidate the underlying mechanisms involved. Human CFs were incubated with or without curcumin (20 µmol/l) and transforming growth factor β1 (TGF‑β1; 10 ng/ml), and the expression of α‑smooth muscle actin (α‑SMA), collagen type Iα (COLA)‑1 and COLA3 was evaluated using reverse transcription‑quantitative polymerase chain reaction and western blot analysis...
June 1, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29901118/the-effect-of-mg%C3%A2-2zn%C3%A2-0-5nd-alloy-on-the-mtor-signalling-pathway-in-l6-cells
#19
Wei Lu, Lei Guo, Tianlong Jiang, Shenghui Ni, Yuyan Zhao
Magnesium alloys have shown potential as biodegradable metallic materials for orthopaedic applications due to their degradability, and their resemblance to cortical bone and biocompatible degradation/corrosion products. However, the fast corrosion rate and the potential toxicity of their alloying element has limited the clinical application of Mg alloys. In the present study, a novel Mg‑2Zn‑0.5Nd alloy was prepared, and then the effects on the cell biological behaviour of the Mg‑2Zn‑0.5Nd alloy was compared with 317L stainless steel and titanium (Ti‑6Al‑4V) alloys as controls...
May 30, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29901107/autophagy-protects-bone-marrow-mesenchymal-stem-cells-from-palmitate%C3%A2-induced-apoptosis-through-the-ros%C3%A2-jnk-p38-mapk-signaling-pathways
#20
Yongyi Liu, Ning Wang, Shaokun Zhang, Qingwei Liang
In recent years, the association between saturated fatty acids (FA) and bone cells has received a high level of attention. Previous studies have shown that palmitate (PA), a common saturated FA, can cause apoptosis in bone marrow mesenchymal stem cells (BMSCs). However, whether PA can induce autophagy, an important intracellular protection mechanism that is closely associated with apoptosis, in BMSCs is still unknown; the association between autophagy and apoptosis is also unclear. The aim of the present study was to determine whether PA can induce autophagy in BMSCs...
May 30, 2018: Molecular Medicine Reports
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