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https://www.readbyqxmd.com/read/29642593/measuring-the-human-ultra-weak-photon-emission-distribution-using-an-electron-multiplying-charge-coupled-device-as-a-sensor
#1
Fernando Ortega-Ojeda, Matías Calcerrada, Alejandro Ferrero, Joaquín Campos, Carmen Garcia-Ruiz
Ultra-weak photon emission (UPE) is the spontaneous emission from living systems mainly attributed to oxidation reactions, in which reactive oxygen species (ROS) may play a major role. Given the capability of the next-generation electron-multiplying CCD (EMCCD) sensors and the easy use of liquid crystal tunable filters (LCTF), the aim of this work was to explore the potential of a simple UPE spectrometer to measure the UPE from a human hand. Thus, an easy setup was configured based on a dark box for inserting the subject's hand prior to LCTF as a monochromator and an EMCCD sensor working in the full vertical binning mode (FVB) as a spectra detector...
April 10, 2018: Sensors
https://www.readbyqxmd.com/read/29642591/light-emission-from-the-fe-2-egta-h%C3%A2-o%C3%A2-system-possible-application-for-the-determination-of-antioxidant-activity-of-plant-phenolics
#2
Michal Nowak, Wieslaw Tryniszewski, Agata Sarniak, Anna Wlodarczyk, Piotr J Nowak, Dariusz Nowak
Oxidative reactions can result in the formation of electronically excited species that undergo radiative decay depending on electronic transition from the excited state to the ground state with subsequent ultra-weak photon emission (UPE). We investigated the UPE from the Fe2+ -EGTA (ethylene glycol-bis(β-aminoethyl ether)- N , N , N ', N '-tetraacetic acid)-H₂O₂ system with a multitube luminometer (Peltier-cooled photon counter, spectral range 380 to 630 nm). The UPE of 92.6 µmol/L Fe2+ -185.2 µmol/L EGTA-2...
April 10, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29621643/potential-induced-reversible-uptake-release-of-perchlorate-from-wastewater-by-polypyrrole-coni-layered-double-hydroxide-modified-electrode-with-proton-ligand-effect
#3
Yanyan Yang, Xiao Du, Xiaowei An, Shengqi Ding, Feifan Liu, Zhonglin Zhang, Xuli Ma, Xiaogang Hao, Guoqing Guan, Hui Zhang
In this study, CoNi-layered double hydroxide (CoNi-LDH) nanosheets coated conducting polypyrrole (PPy) nanowire was controllably fabricated on Pt plate or carbon cloth by using unipolar pulse electrodeposition (UPED) method and served as a novel electrochemically switched ion exchange (ESIX) hybrid film with proton-ligand effect for the removal of perchlorate anions (ClO4 - ). It is expected that the space among CoNi-LDH nanosheets of the shell could act as the reservoir for the anions while the PPy core serve as the potential-induced element for proton-ligand...
March 29, 2018: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29514605/predictive-value-of-spot-versus-24-hour-measures-of-proteinuria-for-death-end-stage-kidney-disease-or-chronic-kidney-disease-progression
#4
Tracey Ying, Philip Clayton, Chetana Naresh, Steven Chadban
BACKGROUND: Proteinuria is well recognised as a marker of chronic kidney disease (CKD), as a risk factor for progression of CKD among those with known CKD, and as a risk factor for cardiovascular events and death among both the general and CKD populations. Which measure of proteinuria is most predictive of renal events remains uncertain. METHODS: We conducted a prospective study with 144 proteinuric CKD and kidney transplant recipients attending an outpatient clinic of a tertiary care hospital in Australia...
March 7, 2018: BMC Nephrology
https://www.readbyqxmd.com/read/29495522/antimicrobial-monomers-for-polymeric-dental-restoratives-cytotoxicity-and-physicochemical-properties
#5
Diane R Bienek, Stanislav A Frukhtbeyn, Anthony A Giuseppetti, Ugochukwu C Okeke, Drago Skrtic
A trend for the next generation of polymeric dental restoratives is to incorporate multifunctional capabilities to regulate microbial growth and remineralize tooth surfaces. Polymerizable 2-(methacryloyloxy)- N -(2-(methacryloyloxy)ethyl)- N , N -dimethylethan-1-aminium bromide (IDMA1) and N , N '-([1,1'-biphenyl]-2,2'-diylbis(methylene))bis(2-(methacryloyloxy)- N , N -dimethylethan-1-aminium) bromide (IDMA2), intended for utilization in bi-functional antimicrobial and remineralizing composites, were synthesized, purified with an ethanol-diethyl ether-hexane solvent system, and validated by nuclear magnetic resonance (¹H and 13 C NMR) spectroscopy, mass spectrometry, and Fourier-transform infrared spectroscopy...
February 27, 2018: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/29445715/detection-of-ultraweak-photon-emission-upe-from-cells-as-a-tool-for-pathological-studies
#6
A Shanei, Z Alinasab, A Kiani, M A Nematollahi
Objective: It is well-known that all living cells emit ultra-weak photon emission (UPE), which is due to byproducts of chemical reactions in cell metabolisms. It has been shown that Reactive Oxygen Species (ROS) in the cells enhances the UPE intensity. The magnitude of such UPE is extremely weak (i.e. a few to 103 photons/ (sec.cm22 )), and the detection of such ultra-weak signals is hardly possible via sensitive instruments like photomultiplier tube (PMT) that can detect single photons...
December 2017: Journal of Biomedical Physics & Engineering
https://www.readbyqxmd.com/read/29416750/pharmacological-targeting-of-ros-reaction-network-in-myeloid-leukemia-cells-monitored-by-ultra-weak-photon-emission
#7
Rosilene Cristina Rossetto Burgos, Rawi Ramautar, Eduard P A Van Wijk, Thomas Hankemeier, Jan Van Der Greef, Alireza Mashaghi
Acute myeloid leukemia (AML) is a blood cancer that is caused by a disorder of the process that normally generates neutrophils. Function and dysfunction of neutrophils are key to physiologic defense against pathogens as well as pathologies including autoimmunity and cancer. A major mechanism through which neutrophils contribute to health and disease is oxidative burst, which involves rapid release of reactive oxygen species (ROS) generated by a chemical reaction network catalyzed by enzymes including NADPH oxidase and myeloperoxidase (MPO)...
January 5, 2018: Oncotarget
https://www.readbyqxmd.com/read/29385908/the-calm-before-the-storm-clinical-observations-of-middle-east-respiratory-syndrome-mers-patients
#8
Jaffar A Al-Tawfiq, Kareem Hinedi
BACKGROUND: Middle East Respiratory Syndrome Coronavirus (MERS-CoV) infection emerged in 2012. The majority of cases occurred in the Kingdom of Saudi Arabia and the disease carries a high case fatality rate. METHODS: We present three MERS-CoV cases and highlight the salient clinical features and laboratory, and radiographic characteristics. RESULTS: Although all nasopharyngeal samples were negative, MERS CoV infection was confirmed by reverse transcription-polymerase chain reaction of the E gene (UpE) and open reading frame (ORF1b) on sputum samples...
February 1, 2018: Journal of Chemotherapy
https://www.readbyqxmd.com/read/29186375/unilateral-pulmonary-oedema-after-minimally-invasive-mitral-valve-surgery-a-single-centre-experience
#9
Jochen Renner, Ulf Lorenzen, Christoph Borzikowsky, Felix Schoeneich, Jochen Cremer, Assad Haneya, Johannes Hensler, Bernd Panholzer, Katharina Huenges, Ole Broch
OBJECTIVES: Unilateral pulmonary oedema (UPE) is a rare but potentially life-threatening complication that has been described after minimally invasive mitral valve surgery (MICS). Over the last 8 years, we have witnessed, in our institution, several cases of severe UPE requiring immediate postoperative extracorporeal life support after MICS. Reviewing the available literature, data regarding this complication after MICS are rare. Consequently, we decided to retrospectively analyse patients scheduled for MICS in our institution...
April 1, 2018: European Journal of Cardio-thoracic Surgery
https://www.readbyqxmd.com/read/29083537/impact-of-unplanned-events-on-early-postoperative-results-of-minimally-invasive-esophagectomy
#10
Xufeng Guo, Bo Ye, Yu Yang, Yifeng Sun, Rong Hua, Xiaobing Zhang, Teng Mao, Zhigang Li
BACKGROUND: Minimally invasive esophagectomy (MIE) is increasingly performed worldwide. Unplanned events during thoracoscopy or laparoscopy can jeopardize the procedure, sometimes necessitating conversion to open surgery. The aim of this study was to evaluate the impact of unplanned events on early postoperative outcomes after MIE. METHOD: A consecutive group of 303 patients who underwent MIE between January 2011 and December 2015 were reviewed. The patients were allocated to two groups comprising those with (G-UPE, 85 patients) and without unplanned events (G-Regular, 218 patients)...
January 2018: Thoracic Cancer
https://www.readbyqxmd.com/read/29061378/candidate-recommendations-for-protein-electrophoresis-reporting-from-the-canadian-society-of-clinical-chemists-monoclonal-gammopathy-working-group
#11
Ronald A Booth, Christopher R McCudden, Cynthia M Balion, Ivan M Blasutig, Ihssan Bouhtiauy, Karina Rodriguez-Capote, Peter Catomeris, Pak Cheung Chan, Yu Chen, Christine Collier, Kristin Hauff, Jawahar Kalra, Dailin Li, Dan C Lin, Amy H Lou, Qing H Meng, Tracy Morrison, Maria D Pasic, Mabood Qureshi, Ed Randell, Kun-Young Sohn, Vinita Thakur, Dylan Thomas, Andrea Thoni, Cheryl Tomalty, Liju Yang, Mohebullah Zamkanei
Protein electrophoresis is commonly used as an aid in the diagnosis of monoclonal gammopathies and is performed in many laboratories in Canada and throughout the world. However, unlike many other diagnostic tests, there is limited guidance for standardization and neither guidance nor specific recommendations for clinical reporting of serum (SPE) or urine (UPE) protein electrophoresis and immunotyping available in the literature. Therefore, a Canadian effort was undertaken to recommend standards that cover all aspects of clinical reporting with an ultimate goal towards reporting standardization...
January 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/28926741/cellular-glutathione-levels-in-hl-60-cells-during-respiratory-burst-are-not-correlated-with-ultra-weak-photon-emission
#12
Rosilene Cristina Rossetto Burgos, Wei Zhang, Eduard P A van Wijk, Thomas Hankemeier, Rawi Ramautar, Jan van der Greef
Recently, ultra-weak photon emission (UPE) was developed as a novel tool for measuring oxidative metabolic processes, as its generation is related to reactive oxygen species (ROS). Both an imbalance in ROS or the uncontrolled production of ROS can lead to oxidative stress, which is commonly associated with many diseases. In addition to playing several biological functions, the thiol amino acid glutathione has an important antioxidant function in the body's defense against ROS. Specifically, glutathione is an important endogenous antioxidant that helps maintain oxidant levels...
September 7, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28917262/determination-of-several-element-levels-in-hardaliye-beverages-using-flame-atomic-absorption-spectrometry-after-ultrasound-probe-extraction
#13
Dilek Bakircioglu, Nukte Topraksever, Yasemin Bakircioglu Kurtulus
In the present study, concentrations of calcium (Ca), copper (Cu), magnesium (Mg), manganese (Mn), sodium (Na), and zinc (Zn) in hardaliye samples produced in Turkey were determined by flame atomic absorption spectrometry after ultrasound probe extraction (UPE), microwave-assisted extraction (MAE), and wet extraction procedures. At present, there is limited work in the literature on UPE for the determination of trace elements in beverage samples. Our single-correlation analysis showed that the elements studied with the UPE method in hardaliye were strongly correlated with the MAE procedure...
September 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28900100/oscillations-of-ultra-weak-photon-emission-from-cancer-and-non-cancer-cells-stressed-by-culture-medium-change-and-tnf-%C3%AE
#14
Pierre Madl, Thomas Verwanger, Mark Geppert, Felix Scholkmann
Cells spontaneously emit photons in the UV to visible/near-infrared range (ultra-weak photon emission, UPE). Perturbations of the cells' state cause changes in UPE (evoked UPE). The aim of the present study was to analyze the evoked UPE dynamics of cells caused by two types of cell perturbations (stressors): (i) a cell culture medium change, and (ii) application of the pro-inflammatory cytokine tumor necrosis factor alpha (TNF-α). Four types of human cell lines were used (squamous cell carcinoma cells, A431; adenocarcinomic alveolar basal epithelial cells, A549; p53-deficient keratinocytes, HaCaT, and cervical cancer cells, HeLa)...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28865316/oxidative-species-induced-excitonic-transport-in-tubulin-aromatic-networks-potential-implications-for-neurodegenerative-disease
#15
P Kurian, T O Obisesan, T J A Craddock
Oxidative stress is a pathological hallmark of neurodegenerative tauopathic disorders such as Alzheimer's disease and Parkinson's disease-related dementia, which are characterized by altered forms of the microtubule-associated protein (MAP) tau. MAP tau is a key protein in stabilizing the microtubule architecture that regulates neuron morphology and synaptic strength. When MAP tau is degraded in tauopathic disorders, neuron dysfunction results. The precise role of reactive oxygen species (ROS) in the tauopathic disease process, however, is poorly understood...
October 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28864832/mri-findings-associated-with-microscopic-residual-tumor-following-unplanned-excision-of-soft-tissue-sarcomas-in-the-extremities
#16
Lee Wang, Juan Pretell-Mazzini, Darcy A Kerr, Lydia Chelala, Xuan Yang, Jean Jose, Ty K Subhawong
INTRODUCTION: MRI is often used to determine the presence of residual disease following unplanned excisions (UPE) of soft tissue sarcomas (STS). We sought to identify MRI features associated with histologic evidence of residual disease after TBE. MATERIALS AND METHODS: This was an IRB-approved retrospective review of 27 patients with R1-type UPE of STS over a 32-month period, with subsequent MRI and TBE. MRI studies were retrospectively evaluated to determine depth of tissue involvement, presence of nodular enhancement, and maximum length of soft tissue edema normalized to extremity size...
February 2018: Skeletal Radiology
https://www.readbyqxmd.com/read/28840986/performance-evaluation-of-the-powerchek-mers-upe-orf1a-real-time-pcr-kit-for-the-detection-of-middle-east-respiratory-syndrome-coronavirus-rna
#17
Hee Jae Huh, Ji Youn Kim, Hyeon Jeong Kwon, Sun Ae Yun, Myoung Keun Lee, Chang Seok Ki, Nam Yong Lee, Jong Won Kim
BACKGROUND: Molecular detection of Middle East respiratory syndrome coronavirus (MERS-CoV) using real-time reverse transcription (rRT)-PCR assays is the method of choice for diagnosis of MERS. We evaluated the performance of the PowerChek MERS (upE & ORF1a) real-time PCR Kit (PowerChek MERS assay; Kogene Biotech, Korea) a one-step rRT-PCR assay for the qualitative detection of MERS-CoV. METHODS: We evaluated PowerChek MERS assay performance in comparison with nested RT-PCR and sequencing of the RNA-dependent RNA polymerase (RdRp) and N genes...
November 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28807812/evaluation-and-clinical-validation-of-two-field-deployable-reverse-transcription-insulated-isothermal-pcr-assays-for-the-detection-of-the-middle-east-respiratory-syndrome-coronavirus
#18
Yun Young Go, Yeon-Sook Kim, Shinhye Cheon, Sangwoo Nam, Keun Bon Ku, Meehyein Kim, Nam Hyuk Cho, Hyun Park, Pei-Yu Alison Lee, Yu-Chun Lin, Yun-Long Tsai, Hwa-Tang Thomas Wang, Udeni B R Balasuriya
Middle East respiratory syndrome (MERS) is an emerging zoonotic viral respiratory disease that was first identified in Saudi Arabia in 2012. In 2015, the largest MERS outbreak outside of the Middle East region occurred in the Republic of Korea. The rapid nosocomial transmission of MERS coronavirus (MERS-CoV) in Korean health care settings highlighted the importance and urgent need for a rapid and reliable on-site diagnostic assay to implement effective control and preventive measures. Here, the evaluation and validation of two newly developed reverse transcription-insulated isothermal PCR (RT-iiPCR) methods targeting the ORF1a and upE genes of MERS-CoV are described...
August 12, 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28616218/soluble-klotho-may-be-a-marker-of-phosphate-reabsorption
#19
Sven-Jean Tan, Edward R Smith, Stephen G Holt, Tim D Hewitson, Nigel D Toussaint
Background: Membrane-bound α-klotho functions as a co-receptor with fibroblast growth factor receptor at the renal tubule conferring specificity to fibroblast growth factor-23 (FGF-23), allowing it to inhibit tubular phosphate reabsorption at physiological concentrations. α-klotho also exists as a soluble protein. However, the complex interrelationships between soluble α-klotho (sKl), FGF-23 and phosphate reabsorption are poorly understood, with little known about the links between sKl, FGF-23 and phosphate reabsorption in chronic kidney disease (CKD)...
June 2017: Clinical Kidney Journal
https://www.readbyqxmd.com/read/28577115/the-psychiatry-major-a-curricular-innovation-to-improve-undergraduate-psychiatry-education-in-china
#20
Ling Jing, Wing Chung Chang, Robert Rohrbaugh, Xuan Ouyang, Eric Chen, Zhening Liu, Xinran Hu
OBJECTIVE: In China, a psychiatry major curriculum (PMC) has been implemented in select medical schools to improve the quality of undergraduate psychiatry education (UPE). Our aim was to describe this PMC and compare it with UPE in the standard Chinese clinical medicine curriculum (CMC). We also benchmarked PMC to UPE programs in the Hong Kong Special Administrative Region of China and the United States of America (USA) to determine how well it met standards of well-established programs and to highlight areas for improvement...
June 2018: Academic Psychiatry
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