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https://www.readbyqxmd.com/read/28431296/survival-and-transfer-efficacy-of-mixed-strain-salmonella-enterica-ser-typhimurium-from-beef-burgers-to-abiotic-surfaces-and-determination-of-individual-strain-contribution
#1
E N Gkana, A I Doulgeraki, G-J E Nychas
The aim of the study was to evaluate the survival and transfer efficacy of 3 Salmonella Typhimurium strains from beef burgers to abiotic surfaces and determine the individual strain distribution. S. Typhimurium population on beef burgers during incubation remained constant at initial levels of contamination approximately 3 and 5 log CFU/g. Additionally, the survival of pathogens on soiled HDPE surfaces was significant during incubation at both initial inocula, while ca 1.5 log CFU/cm(2) reduction was observed at 168h...
April 7, 2017: Meat Science
https://www.readbyqxmd.com/read/28431183/efficacy-of-tiamulin-alone-or-in-combination-with-chlortetracycline-against-experimental-mycoplasma-gallisepticum-infection-in-chickens
#2
A Garmyn, M Vereecken, K Degussem, W Depondt, F Haesebrouck, A Martel
Mycoplasma gallisepticum (M. gallisepticum) remains one of the most important diseases in poultry production. Controlling the impact of the disease is done by eradication of positive breeder flocks or by vaccination and medication. A widely used molecule in medication programs is tiamulin, a pleuromutilin antibiotic. Since recent data on the in vivo efficacy of this molecule are scarce, 2 challenge studies were conducted using a recently isolated M. gallisepticum strain belonging to the wildtype population with regard to its tiamulin and tetracycline minimum inhibitory concentration (MIC)...
April 20, 2017: Poultry Science
https://www.readbyqxmd.com/read/28431154/physiological-characterization-of-a-halotolerant-anoxygenic-phototrophic-fe-ii-oxidizing-green-sulfur-bacterium-isolated-from-a-marine-sediment
#3
Katja Laufer, Annika Niemeyer, Verena Nikeleit, Maximilian Halama, James M Byrne, Andreas Kappler
Anoxygenic photoautotrophic bacteria which use light energy and electrons from Fe(II) for growth, so-called photoferrotrophs, are suggested to have been amongst the first phototrophic microorganisms on Earth and to have contributed to the deposition of sedimentary iron mineral deposits, i.e. banded iron formations. To date only two isolates of marine photoferrotrophic bacteria exist, both of which are closely related purple non-sulfur bacteria. Here we present a novel green-sulfur photoautotrophic Fe(II)-oxidizer isolated from a marine coastal sediment, Chlorobium sp...
April 18, 2017: FEMS Microbiology Ecology
https://www.readbyqxmd.com/read/28431114/molecular-characterization-of-cryptococcus-neoformans-isolated-from-the-environment-in-beijing-china
#4
Hongtao Dou, Huizhu Wang, Shaowei Xie, Xinxin Chen, Zhipeng Xu, Yingchun Xu
The molecular type of environmental Cryptococcus neoformans in Beijing was not clear. Our study aims to reveal the molecular characterization of C. neoformans complex from environment in Beijing, China. A total of 435 samples of pigeon droppings from 11 different homes in Beijing were collected from August to November in 2015. Pigeon droppings were inoculated onto caffeic acid cornmeal agar (CACA) to screen C. neoformans complex. Bruker Biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was performed for species identification...
April 20, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28431066/limited-left-atrial-surgical-ablation-effectively-treats-atrial-fibrillation-but-decreases-left-atrial-function
#5
Marieke G Compier, Laurens F Tops, Jerry Braun, Katja Zeppenfeld, Robert J Klautz, Martin J Schalij, Serge A Trines
Aims: Limited left atrial (LA) surgical ablation with bipolar radiofrequency is considered to be an effective procedure for treatment of atrial fibrillation (AF). We studied whether limited LA surgical ablation concomitant to cardiac surgery is able to maintain LA function. Methods and Results: Thirty-six consecutive patients (age 66 ± 12 years, 53% male, 78% persistent AF) scheduled for valve surgery and/or coronary revascularization and concomitant LA surgical ablation were included...
April 1, 2017: Europace: European Pacing, Arrhythmias, and Cardiac Electrophysiology
https://www.readbyqxmd.com/read/28431022/cpp1-phosphatase-mediated-signaling-crosstalk-between-hog1-and-cek1-mitogen-activated-protein-kinases-is-involved-in-the-phenotypic-transition-in-candida-albicans
#6
Fu-Sheng Deng, Ching-Hsuan Lin
Cellular signaling pathways involved in cell growth and differentiation mediated by mitogen-activated protein kinase (MAPK) cascades have been well characterized in fungi. However, the mechanisms of signaling crosstalk between MAPKs to ensure signaling specificity are largely unknown. Previous work showed that activation of the Candida albicans Cek1 MAPK pathway resulted in opaque cell formation and filamentation, which mirrored the phenotypes to hog1Δ. Additionally, deleting the HOG1 gene stimulated Cek1p...
April 20, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/28430186/independence-of-nutrient-limitation-and-carbon-dioxide-impacts-on-the-southern-ocean-coccolithophore-emiliania-huxleyi
#7
Marius N Müller, Thomas W Trull, Gustaaf M Hallegraeff
Future oceanic conditions induced by anthropogenic greenhouse gas emissions include warming, acidification and reduced nutrient supply due to increased stratification. Some parts of the Southern Ocean are expected to show rapid changes, especially for carbonate mineral saturation. Here we compare the physiological response of the model coccolithophore Emiliania huxleyi (strain EHSO 5.14, originating from 50(o)S, 149(o)E) with pH/CO2 gradients (mimicking ocean acidification ranging from 1 to 4 × current pCO2 levels) under nutrient-limited (nitrogen and phosphorus) and -replete conditions...
April 21, 2017: ISME Journal
https://www.readbyqxmd.com/read/28429692/pulmonary-artery-and-right-ventricle-function-in-patients-with-bicuspid-aortic-valve
#8
Çetin Geçmen, Gamze Babür Güler, Suzan Hatipoğlu, Muzaffer Kahyaoğlu, Murat Çap, Servet İzci, Çağatay Önal, Emrah Erdoğan, Aykun Hakgör, Özkan Candan, Arzu Kalaycı, Tuba Unkun, İbrahim Akın İzgi
OBJECTIVE: Bicuspid aortic valve (BAV) is a complex developmental anomaly caused by abnormal aortic leaflet formation during valvulogenesis. The present study is an assessment of the effects of BAV disease on the ascending aorta and pulmonary artery (PA), and an evaluation of the consequences for systolic and diastolic functioning of the left and right ventricles. METHODS: Total of 66 patients were eligible for inclusion. Pulmonary artery maximum diameter (PAD) was obtained 1 cm distal to the pulmonary annulus...
April 2017: Türk Kardiyoloji Derneği Arşivi: Türk Kardiyoloji Derneğinin Yayın Organıdır
https://www.readbyqxmd.com/read/28429529/impaired-left-atrial-function-predicts-inappropriate-shocks-in-primary-prevention-implantable-cardioverter-defibrillator-candidates
#9
Susumu Tao, Hiroshi Ashikaga, Luisa A Ciuffo, Kihei Yoneyama, Joao A C Lima, Terry F Frank, Robert G Weiss, Gordon F Tomaselli, Katherine C Wu
INTRODUCTION: Inappropriate implantable cardioverter-defibrillator (ICD) shocks, commonly caused by atrial fibrillation (AF), are associated with an increased mortality. Because impaired left atrial (LA) function predicts development of AF, we hypothesized that impaired LA function predicts inappropriate shocks beyond a history of AF. METHODS AND RESULTS: We prospectively analyzed the association between LA function and incident inappropriate shocks in primary prevention ICD candidates...
April 21, 2017: Journal of Cardiovascular Electrophysiology
https://www.readbyqxmd.com/read/28429279/improvement-of-lipid-production-by-the-oleaginous-yeast-rhodosporidium-toruloides-through-uv-mutagenesis
#10
Ryosuke Yamada, Tomomi Kashihara, Hiroyasu Ogino
Oleaginous yeasts are considered a promising alternative lipid source for biodiesel fuel production. In this study, we attempted to improve the lipid productivity of the oleaginous yeast Rhodosporidium toruloides through UV irradiation mutagenesis and selection based on ethanol and H2O2 tolerance or cerulenin, a fatty acid synthetase inhibitor. Glucose consumption, cell growth, and lipid production of mutants were evaluated. The transcription level of genes involved in lipid production was also evaluated in mutants...
May 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28429161/on-the-in-vivo-function-of-the-mitral-heart-valve-leaflet-insights-into-tissue-interstitial-cell-biomechanical-coupling
#11
Chung-Hao Lee, Will Zhang, Kristen Feaver, Robert C Gorman, Joseph H Gorman, Michael S Sacks
There continues to be a critical need for developing data-informed computational modeling techniques that enable systematic evaluations of mitral valve (MV) function. This is important for a better understanding of MV organ-level biomechanical performance, in vivo functional tissue stresses, and the biosynthetic responses of MV interstitial cells (MVICs) in the normal, pathophysiological, and surgically repaired states. In the present study, we utilized extant ovine MV population-averaged 3D fiducial marker data to quantify the MV anterior leaflet (MVAL) deformations in various kinematic states...
April 20, 2017: Biomechanics and Modeling in Mechanobiology
https://www.readbyqxmd.com/read/28429151/corynebacterium-species-nasopharyngeal-carriage-in-asymptomatic-individuals-aged%C3%A2-%C3%A2-%C3%A2-65%C3%A2-years-in-germany
#12
Barbara Teutsch, Anja Berger, Durdica Marosevic, Katharina Schönberger, Thiên-Trí Lâm, Kerstin Hubert, Steffi Beer, Peter Wienert, Nikolaus Ackermann, Heike Claus, Maria Drayß, Kathrin Thiel, Mark van der Linden, Ulrich Vogel, Andreas Sing
PURPOSE: The prevalence of protective anti-diphtheria toxin antibodies decreases with age. Therefore, the elderly might serve as reservoir for potentially toxigenic Corynebacterium (C.) species (C. diphtheriae, C. ulcerans, and C. pseudotuberculosis). This study aimed to examine the colonization rate of the nasopharynx with corynebacteria of individuals aged 65 years and older. METHODS: In the period from October 2012 to June 2013, nasal and throat swabs were taken from 714 asymptomatic subjects aged 65-106 years (average age 77...
April 20, 2017: Infection
https://www.readbyqxmd.com/read/28429058/effects-of-genome-duplication-on-phenotypes-and-industrial-applications-of-saccharomyces-cerevisiae-strains
#13
Ke Zhang, Ya-Hong Fang, Ke-Hui Gao, Yang Sui, Dao-Qiong Zheng, Xue-Chang Wu
Polyploidy is common in Saccharomyces cerevisiae strains, but the physiological and phenotypic effects of ploidy changes have not been fully clarified. Here, isogenic diploid, triploid, and tetraploid S. cerevisiae strains were constructed from a haploid strain, CEN.PK2-1C. Stress tolerance and ethanol fermentation performance of the four euploid strains were compared. Each euploid strain had strengths and weaknesses in tolerance to certain stressors, and no single strain was tolerant of all stressors. The diploid had higher ethanol production than the other strains in normal fermentation medium, while the triploid strain showed the fastest fermentation rate in the presence of inhibitors found in lignocellulosic hydrolysate...
April 20, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28428315/improved-high-quality-draft-genome-sequence-and-annotation-of-burkholderia-contaminans-lmg-23361-t
#14
Ji Young Jung, Youngbeom Ahn, Ohgew Kweon, John J LiPuma, David Hussong, Bernard S Marasa, Carl E Cerniglia
Burkholderia contaminans LMG 23361 is the type strain of the species isolated from the milk of a dairy sheep with mastitis. Some pharmaceutical products contain disinfectants such as benzalkonium chloride (BZK) and previously we reported that B. contaminans LMG 23361(T) possesses the ability to inactivate BZK with high biodegradation rates. Here, we report an improved high-quality draft genome sequence of this strain.
April 20, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28428307/draft-genome-sequences-of-three-listeria-monocytogenes-isolates-from-foods-in-china
#15
Xudong Su, Guojie Cao, Dai Kuang, Jianmin Zhang, Yi Chen, Marc Allard, Eric Brown, Xianming Shi, Jianghong Meng, Xuebin Xu
Listeria monocytogenes is a foodborne pathogen of global concern because of the high mortality rate among patients. The draft genome sequences of three L. monocytogenes strains isolated from foods are reported here. The availability of these genomes should provide useful information on the genomic diversity of L. monocytogenes isolated from foods in China.
April 20, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28427450/presence-of-susceptible-wild-strains-of-anopheles-gambiae-in-a-large-industrial-palm-farm-located-in-aboisso-south-eastern-of-c%C3%A3-te-d-ivoire
#16
Cécile M A Sadia-Kacou, Ludovic P Ahoua Alou, Ako V C Edi, Celine M Yobo, Maurice A Adja, Allassane F Ouattara, David Malone, Alphonsine A Koffi, Yao Tano, Benjamin G Koudou
BACKGROUND: The effectiveness of malaria control programmes through implementation of vector control activities is challenged by the emergence of insecticide resistance. In the South-Eastern region of Côte d'Ivoire, where palm oil plantations remain the predominant agricultural crop, the susceptibility of wild Anopheles gambiae sensu lato species is still unknown and thus requires a particular attention. The current study was carried out to address the gap by in-depth characterization of susceptibility level of An...
April 20, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28426933/triggering-assembly-and-disassembly-of-a-supramolecular-cage
#17
Carlo Bravin, Elena Badetti, Francesca Anna Scaramuzzo, Giulia Licini, Cristiano Zonta
A novel supramolecular cage built from the self-assembly of tris(2-pyridylmethyl)amine TPMA zinc complexes through imine condensation chemistry is reported. The cage recognition properties over a variety of structurally related guests, together with the kinetic study of the template assembly and disassembly, have been investigated in detail. This knowledge has been used to selectively modulate the rate of both assembly and disassembly processes. In particular, a novel disassembly method induced by strain release of the guest has been developed...
April 20, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28426720/a-new-model-mimicking-persistent-hbv-e-antigen-negative-infection-using-covalently-closed-circular-dna-in-immunocompetent-mice
#18
Lei Wang, Min Cao, Qing Lu Wei, Zhong Hua Zhao, Qin Xiang, Hui Juan Wang, Hua Tang Zhang, Guo Qi Lai
Despite the availability of an effective vaccine, hepatitis B virus (HBV) infection remains a major health problem. HBV e antigen (HBeAg)-negative strains have become prevalent. Previously, no animal model mimicked the clinical course of HBeAg-negative HBV infection. To establish an HBeAg-negative HBV infection model, the 3.2-kb full-length genome of HBeAg-negative HBV was cloned from a clinical sample and then circularized to form covalently closed circular (cccDNA). The resulting cccDNA was introduced into the liver of C57BL/6J mice through hydrodynamic injection...
2017: PloS One
https://www.readbyqxmd.com/read/28426144/a-multiplex-culture-system-for-the-long-term-growth-of-fission-yeast-cells
#19
Celine Callens, Nelson C Coelho, Aaron W Miller, Maria Rosa Domino Sananes, Maitreya J Dunham, Matthieu Denoual, Damien Coudreuse
Maintenance of long-term cultures of yeast cells is central to a broad range of investigations, from metabolic studies to laboratory evolution assays. However, repeated dilutions of batch cultures lead to variations in medium composition, with implications for cell physiology. In S. cerevisiae, powerful miniaturized chemostat setups, or ministat arrays, have been shown to allow for constant dilution of multiple independent cultures. Here we set out to adapt these arrays for continuous culture of a morphologically and physiologically distinct yeast, the fission yeast Schizosaccharomyces pombe, with the goal of maintaining constant population density over time...
April 20, 2017: Yeast
https://www.readbyqxmd.com/read/28425834/rift-valley-fever-vaccines-an-overview-of-the-safety-and-efficacy-of-the-live-attenuated-mp-12-vaccine-candidate
#20
Tetsuro Ikegami
Rift Valley fever (RVF) is a mosquito-borne zoonotic viral disease endemic to Africa and the Arabian Peninsula. High rates of abortion among infected ruminants and hemorrhagic fever in infected humans are major public health concerns. Commercially available veterinary RVF vaccines are important for preventing the spread of the Rift Valley fever virus (RVFV) in endemic countries; however, RVFV outbreaks continue to occur frequently in endemic countries in the 21(st) century. In the U.S., the live-attenuated MP-12 vaccine has been developed for both animal and human vaccination...
April 20, 2017: Expert Review of Vaccines
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