keyword
MENU ▼
Read by QxMD icon Read
search

MEF

keyword
https://www.readbyqxmd.com/read/29344644/inactivation-of-dna-pk-by-knockdown-dna-pkcs-or-nu7441-impairs-non-homologous-end-joining-of-radiation-induced-double-strand-break-repair
#1
Jun Dong, Yufeng Ren, Tian Zhang, Zhenyu Wang, Clifton C Ling, Gloria C Li, Fuqiu He, Chengtao Wang, Bixiu Wen
The DNA-dependent protein kinase (DNA-PK) complex plays a pivotal role in non-homologous end-joining (NHEJ) repair. We investigated the mechanism of NU7441, a highly selective DNA-PK inhibitor, in NHEJ-competent mouse embryonic fibroblast (MEF) cells and NHEJ-deficient cells and explored the feasibility of its application in radiosensitizing nasopharyngeal carcinoma (NPC) cells. We generated wild-type and DNA-PKcs-/- MEF cells. Clonogenic survival assays, flow cytometry, and immunoblotting were performed to study the effect of NU7441 on survival, cell cycle, and DNA repair...
January 16, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29342503/the-molecular-basis-for-dysregulated-activation-of-nkx2-5-in-vascular-remodelling-of-systemic-sclerosis
#2
Athina Dritsoula, Ioannis Papaioannou, Sandra G Guerra, Carmen Fonseca, Javier Martin, Ariane L Herrick, David J Abraham, Christopher P Denton, Markella Ponticos
OBJECTIVE: NKX2-5 is a homeobox transcription factor required for the formation of the heart and vessels during development, with significant postnatal downregulation and reactivation in disease states characterised by vascular remodelling. In this study, we sought to investigate mechanisms that activate NKX2-5 expression in diseased vessels, such as scleroderma associated pulmonary hypertension (SSc-PH), and identify genetic variability that potentially underlies susceptibility to specific vascular complications...
January 17, 2018: Arthritis & Rheumatology
https://www.readbyqxmd.com/read/29338847/evaluation-and-validation-of-musculoskeletal-force-feasible-set-indices-application-to-manual-wheelchair-propulsion
#3
Vincent Hernandez, Philippe Gorce, Nasser Rezzoug
The aim of this work was to assess handrim wheelchair propulsion effectiveness, related to the applied forces on the handrim, through the force feasible set. For a given posture of the upper-limb, it represents the set of isometric forces that can be applied on the handrim in any direction. The force feasible set was predicted from a musculoskeletal model of the upper-limb and trunk (10 degrees of freedom and 56 muscles). The aim of the first part of the study was to compare the force feasible set prediction and the force currently applied on the handrim...
December 15, 2017: Journal of Biomechanics
https://www.readbyqxmd.com/read/29338005/inferring-cell-state-by-quantitative-motility-analysis-reveals-a-dynamic-state-system-and-broken-detailed-balance
#4
Jacob C Kimmel, Amy Y Chang, Andrew S Brack, Wallace F Marshall
Cell populations display heterogeneous and dynamic phenotypic states at multiple scales. Similar to molecular features commonly used to explore cell heterogeneity, cell behavior is a rich phenotypic space that may allow for identification of relevant cell states. Inference of cell state from cell behavior across a time course may enable the investigation of dynamics of transitions between heterogeneous cell states, a task difficult to perform with destructive molecular observations. Cell motility is one such easily observed cell behavior with known biomedical relevance...
January 16, 2018: PLoS Computational Biology
https://www.readbyqxmd.com/read/29337003/mouse-lung-fibroblasts-are-highly-susceptible-to-necroptosis-in-a-reactive-oxygen-species-dependent-manner
#5
Muadh Hussain, Vanessa Zimmermann, Sjoerd J L van Wijk, Simone Fulda
Mouse embryonic fibroblasts (MEFs) have extensively been used to study necroptosis, a recently identified form of programmed cell death. However, very little is yet known about the role of necroptosis and its regulation by reactive oxygen species (ROS) in cell types naturally exposed to high oxygen levels such as mouse lung fibroblasts (MLFs). Here, we discover that MLFs are highly susceptible to undergo necroptosis in a ROS-dependent manner upon exposure to a prototypic death receptor-mediated necroptotic stimulus, i...
January 11, 2018: Biochemical Pharmacology
https://www.readbyqxmd.com/read/29330306/an-rb-family-independent-e2f3-transcription-factor-variant-impairs-stat5-signaling-and-mammary-gland-remodeling-during-pregnancy-in-mice
#6
Yang Liao, Wei Du
E2F transcription factors are regulated by binding to the retinoblastoma (Rb) tumor suppressor family of proteins. Previously, we reported an E2FLQ mutation that disrupts the binding with Rb proteins without affecting E2F's transcriptional activity. We also showed that mouse embryonic fibroblasts (MEFs) with an E2F3LQ mutation exhibit increased E2F activity and more rapid cell proliferation. In this report, we analyzed E2F3LQ mice to further characterize the in vivo consequences of Rb family-independent E2F3 activity...
January 12, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29312817/murine-pluripotent-stem-cells-that-escape-differentiation-inside-teratomas-maintain-pluripotency
#7
Yangli Pei, Liang Yue, Wei Zhang, Jinzhu Xiang, Zhu Ma, Jianyong Han
Background: Pluripotent stem cells (PSCs) offer immense potential as a source for regenerative therapies. The teratoma assay is widely used in the field of stem cells and regenerative medicine, but the cell composition of teratoma is still elusive. Methods: We utilized PSCs expressing enhanced green fluorescent protein (EGFP) under the control of the Pou5f1 promoter to study the persistence of potential pluripotent cells during teratoma formation in vivo. OCT4-MES (mouse embryonic stem cells) were isolated from the blastocysts of 3...
2018: PeerJ
https://www.readbyqxmd.com/read/29312622/acute-nutritional-stress-during-pregnancy-affects-placental-efficiency-fetal-growth-and-adult-glucose-homeostasis
#8
Sajida Malik, Alan Diot, Karl Morten, Eszter Dombi, Manu Vatish, C A Richard Boyd, Joanna Poulton
Exposure to maternal malnutrition impairs postnatal health. Acute nutritional stress is less clearly implicated in intrauterine programming. We studied the effects of stressing pregnant mothers on perinatal growth and adult glucose homeostasis. We compared one group ("stressed", mothers fasted for 16 hours) with controls ("unstressed"). We found that fasting stress had adverse effects on the weight of the fetuses conceived (p<0.005) and the placental efficiency (p<0.001) in stressed compared to unstressed offspring...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29291401/wound-healing-related-properties-detected-in-an-experimental-model-with-a-collagen-gel-contraction-assay-are-affected-in-the-absence-of-tenascin-x
#9
Kei Hashimoto, Naoyo Kajitani, Yasunori Miyamoto, Ken-Ichi Matsumoto
Patients with tenascin-X (TNX)-deficient type Ehlers-Danlos syndrome (EDS) do not exhibit delayed wound healing, unlike classic type EDS patients, who exhibit mutations in collagen genes. Similarly, in TNX-knockout (KO) mice, wound closure of the skin is normal even though these mice exhibit a reduced breaking strength. Therefore, we speculated that the wound healing process may be affected in the absence of TNX. In this study, to investigate the effects of TNX absence on wound healing-related properties, we performed collagen gel contraction assays with wild-type (WT) and TNX-KO mouse embryonic fibroblasts (MEFs)...
December 29, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/29289385/phenotypic-and-molecular-characterization-of-streptococcus-pneumoniae-in-pre-conjugate-vaccine-era-a-chinese-hospital-based-retrospective-study
#10
Ling Li, Jinjian Fu, Shunming Li, Dan Guo, Zhiyao Chen, Sidong Chen, Xiaohua Ye
BACKGROUND: Streptococcus pneumoniae (S. pneumoniae) is an important pathogen in causing global morbidity and mortality among children. This study aimed to determine phenotypic and molecular characteristics of S. pneumoniae causing infections in children under five years in China. METHODS: A hospital-based retrospective study was conducted. All 537 S. pneumoniae isolates were tested for antimicrobial susceptibility by E-test method, molecular characteristics including resistance genes, virulence genes and serotypes by multiplex polymerase chain reaction (PCR) method, and sequence types (STs) by sequencing seven housekeeping genes...
December 27, 2017: Vaccine
https://www.readbyqxmd.com/read/29284267/integrating-metal-enhanced-fluorescence-and-surface-acoustic-waves-for-sensitive-and-rapid-quantification-of-cancer-biomarkers-from-real-matrices
#11
Jun Liu, Shuangming Li, Venkat R Bhethanabotla
Metal-enhanced fluorescence (MEF) is utilized to lower the detection limit of carcinoembryonic antigen (CEA), a prognostic biomarker for colorectal cancer among others, in immunofluorescence assays. In addition, Rayleigh surface acoustic waves (SAWs) were utilized to remove non-specifically bound proteins, improve mixing and reduce incubation times. Fluorescence intensity was plasmonically enhanced by incubating silver nanocubes (AgNCs) of 50 nm edge-length on a SAW device. This increased sensor sensitivity by a factor of six and lowered the limit of detection to below 1 ng/mL in fluorescence detection of the antigen...
December 29, 2017: ACS Sensors
https://www.readbyqxmd.com/read/29275121/ve-cadherin-regulates-the-self-renewal-of-mouse-embryonic-stem-cells-via-lif-stat3-signaling-pathway
#12
Ningning He, Xiaoniao Chen, Dan Wang, Ke Xu, Lingling Wu, Yuanyuan Liu, Hongyan Tao, Qinjun Zhao, Xiaocang Cao, Yuhao Li, Na Liu, Xin Qi, Zhongchao Han, Deling Kong, Jun Yang, Zongjin Li
With the abilities of self-renewal and differentiation, embryonic stem (ES) cells provide an unlimited source for stem cell-based therapeutics. However, the maintenance of ES cells with mouse embryonic fibroblasts (MEFs) can limit the clinical translation of ES cells. In the present study, we synthesized a fusion protein of the immunoglobulin G (IgG) fragment crystallizable region and vascular endothelial cadherin (VE-cadherin) extracellular domain (VE-cad-Fc) as a substrate for mouse ES cell culture, and we hypothesized that VE-cadherin could enhance the pluripotency and self-renewal of ES cells...
December 16, 2017: Biomaterials
https://www.readbyqxmd.com/read/29272917/purification-and-characterization-of-peptides-inhibiting-mmp-1-activity-with-c-terminate-of-gly-leu-from-simulated-gastrointestinal-digestion-hydrolysates-of-tilapia-oreochromis-niloticus-skin-gelatin
#13
Sun Liping, Liu Qiuming, Fan Jian, Li Xiao, Zhuang Yongliang
Tilapia skin gelatin hydrolysates (TSGH) were prepared by simulated gastrointestinal digestion and separated by gel filtration and semipreparative reverse phase-high performance liquid chromatography. The antiphotoaging effects were evaluated using an ultraviolet radiation B (UVB)-induced mouse embryonic fibroblasts (MEFs) photoaging model in vitro. Three fractions from TSGH with high inhibitory intercellular matrix metalloproteinases-1 (MMP-1) activities and reactive oxygen species (ROS) production were obtained...
December 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29259016/oncogenic-ras-induced-perinuclear-signaling-complexes-requiring-ksr1-regulate-signal-transmission-to-downstream-targets
#14
Sandip K Basu, Sook Lee, Jacqueline Salotti, Srikanta Basu, Krisada Sakchaisri, Zhen Xiao, Vijay Walia, Christopher J Westlake, Deborah K Morrison, Peter F Johnson
The precise characteristics that distinguish normal and oncogenic RAS signaling remain obscure. Here we show that oncogenic RAS and BRAF induce perinuclear re-localization of several RAS pathway proteins, including the kinases CK2 and p-ERK1/2 and the signaling scaffold KSR1. This spatial reorganization requires endocytosis, the kinase activities of MEK-ERK and CK2, and the presence of KSR1. CK2α co-localizes with KSR1 and Rab11, a marker of recycling endosomes, whereas p-ERK associates predominantly with a distinct KSR1-positive endosomal population...
December 19, 2017: Cancer Research
https://www.readbyqxmd.com/read/29247004/caveolin-1-promotes-the-tumor-suppressor-properties-of-oncogene-induced-cellular-senescence
#15
Daniela Volonte, Avani R Vyas, Chen Chen, Sanja Dacic, Laura P Stabile, Brenda F Kurland, Shira R Abberbock, Timothy F Burns, James M Herman, Yuanpu Peter Di, Ferruccio Galbiati
Oncogene-induced senescence (OIS) is considered a powerful tumor suppressor mechanism. Caveolin-1 acts as a scaffolding protein to functionally regulate signaling molecules. We demonstrate that a lack of caveolin-1 expression inhibits oncogenic K-Ras (K-RasG12V)-induced premature senescence in mouse embryonic fibroblasts (MEFs) and normal human bronchial epithelial (NHBE) cells. Oncogenic K-Ras induces senescence by limiting the detoxification function of MTH1. We find that K-RasG12V promotes the interaction of caveolin-1 with MTH1, which results in inhibition of MTH1 activity...
December 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29236231/dimethyl-fumarate-a-review-in-moderate-to-severe-plaque-psoriasis
#16
Hannah A Blair
Fumaric acid esters (FAEs) have been used in the treatment of psoriasis in some European countries for over 20 years, and are recommended in the European guidelines for the management of moderate to severe plaque psoriasis. Dimethyl fumarate (Skilarence®; hereafter referred to as DMF) is an orally administered FAE indicated for the treatment of moderate to severe plaque psoriasis in adults in need of systemic medicinal therapy; unlike other available FAEs, it is not formulated in combination with monoethyl fumarate salts...
December 13, 2017: Drugs
https://www.readbyqxmd.com/read/29233735/n-terminal-acetylation-modulates-bax-targeting-to-mitochondria
#17
Sara Alves, Leire Neiri, Susana Rodrigues Chaves, Selma Vieira, Dário Trindade, Stephen Manon, Veronica Dominguez, Belen Pintado, Veronique Jonckheere, Petra Van Damme, Rui Duarte Silva, Rafael Aldabe, Manuela Côrte-Real
The pro-apoptotic Bax protein is the main effector of mitochondrial permeabilization during apoptosis. Bax is controlled at several levels, including post-translational modifications such as phosphorylation and S-palmitoylation. However, little is known about the contribution of other protein modifications to Bax activity. Here, we used heterologous expression of human Bax in yeast to study the involvement of N-terminal acetylation by yNaa20p (yNatB) on Bax function. We found that human Bax is N-terminal (Nt-)acetylated by yNaa20p and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of yeast and Mouse Embryonic Fibroblast (MEF) cells...
December 9, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29229671/h3s10ph-broadly-marks-early-replicating-domains-in-interphase-escs-and-shows-reciprocal-antagonism-with-h3k9me2
#18
Carol C L Chen, Preeti Goyal, Mohammad M Karimi, Marie H Abildgaard, Hiroshi Kimura, Matthew C Lorincz
Phosphorylation of histone H3 at serine 10 (H3S10ph) by Aurora kinases plays an important role in mitosis; however, H3S10ph also marks regulatory regions of inducible genes in interphase mammalian cells, implicating mitosis-independent functions. Using the fluorescent ubiquitin-mediated cell cycle indicator (FUCCI), we found that 30% of the genome in interphase mouse embryonic stem cells (ESCs) is marked with H3S10ph. H3S10ph broadly demarcates gene-rich regions in G1 and is positively correlated with domains of early DNA replication timing (RT) but negatively correlated with H3K9me2 and lamin-associated domains (LADs)...
December 11, 2017: Genome Research
https://www.readbyqxmd.com/read/29228832/nadph-oxidase-4-mediates-ros-production-in-radiation-induced-senescent-cells-and-promotes-migration-of-inflammatory-cells
#19
Yuri Sakai, Tohru Yamamori, Yoji Yoshikawa, Tomoki Bo, Motofumi Suzuki, Kumiko Yamamoto, Tetsuro Ago, Osamu Inanami
Excessive DNA damage induced by ionizing radiation (IR) to normal tissue cells is known to trigger cellular senescence, a process termed stress-induced premature senescence (SIPS). SIPS is often accompanied by the production of reactive oxygen species (ROS), and this is reported to be important for the initiation and maintenance of SIPS. However, the source of ROS during SIPS after IR and their significance in radiation-induced normal tissue damage remain elusive. In the present study, we tested the hypothesis that the NADPH oxidase (NOX) family of proteins mediates ROS production in SIPS-induced cells after IR and plays a role in SIPS-associated biological events...
December 12, 2017: Free Radical Research
https://www.readbyqxmd.com/read/29220666/chromatin-accessibility-dynamics-during-ipsc-reprogramming
#20
Dongwei Li, Jing Liu, Xuejie Yang, Chunhua Zhou, Jing Guo, Chuman Wu, Yue Qin, Lin Guo, Jiangping He, Shenyong Yu, He Liu, Xiaoshan Wang, Fang Wu, Junqi Kuang, Andrew P Hutchins, Jiekai Chen, Duanqing Pei
Cell-fate decisions remain poorly understood at the chromatin level. Here, we map chromatin remodeling dynamics during induction of pluripotent stem cells. ATAC-seq profiling of MEFs expressing Oct4-Sox2-Klf4 (OSK) reveals dynamic changes in chromatin states shifting from open to closed (OC) and closed to open (CO), with an initial burst of OC and an ending surge of CO. The OC loci are largely composed of genes associated with a somatic fate, while the CO loci are associated with pluripotency. Factors/conditions known to impede reprogramming prevent OSK-driven OC and skew OC-CO dynamics...
December 7, 2017: Cell Stem Cell
keyword
keyword
49805
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"