Daniëlle de Jong-Bolm, Mohsen Sadeghi, Cristian A Bogaciu, Guobin Bao, Gabriele Klaehn, Merle Hoff, Lucas Mittelmeier, F Buket Basmanav, Felipe Opazo, Frank Noé, Silvio O Rizzoli
Multiplexed cellular imaging typically relies on the sequential application of detection probes, as antibodies or DNA barcodes, which is complex and time-consuming. To address this, we developed here protein nanobarcodes, composed of combinations of epitopes recognized by specific sets of nanobodies. The nanobarcodes are read in a single imaging step, relying on nanobodies conjugated to distinct fluorophores, which enables a precise analysis of large numbers of protein combinations. Fluorescence images from nanobarcodes were used as input images for a deep neural network, which was able to identify proteins with high precision...
December 11, 2023: PLoS Biology