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"Muscle fiber"

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https://www.readbyqxmd.com/read/29053816/histomorphometric-study-of-the-anterior-latissimus-dorsi-muscle-and-evaluation-of-enzymatic-markers-of-broilers-affected-with-dorsal-cranial-myopathy
#1
R Sesterhenn, F M Siqueira, A C Hamerski, D Driemeier, S F Valle, S L Vieira, L Kindlein, V P Nascimento
Dorsal cranial myopathy (DCM), which affects the anterior latissimus dorsi (ALD) muscles of commercial broilers, is of unknown etiology, and it represents up to 6% of the partial condemnations in Brazilian slaughterhouses. This study was performed to achieve histomorphometric characterizations of the ALD muscles from male Cobb 500 broilers slaughtered at either 35 d or 42 d and to evaluate the effects of DCM on the enzymatic markers aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), and lactate dehydrogenase (LDH) and on uric acid and creatinine metabolites...
September 20, 2017: Poultry Science
https://www.readbyqxmd.com/read/29051533/microtome-integrated-microscope-system-for-high-sensitivity-tracking-of-in-resin-fluorescence-in-blocks-and-ultrathin-sections-for-correlative-microscopy
#2
Nicolas Lemercier, Volker Middel, Didier Hentsch, Serge Taubert, Masanari Takamiya, Tanja Beil, Jean-Luc Vonesch, Tilo Baumbach, Patrick Schultz, Claude Antony, Uwe Strähle
Many areas of biological research demand the combined use of different imaging modalities to cover a wide range of magnifications and measurements or to place fluorescent patterns into an ultrastructural context. A technically difficult problem is the efficient specimen transfer between different imaging modalities without losing the coordinates of the regions-of-interest (ROI). Here, we report a new and highly sensitive integrated system that combines a custom designed microscope with an ultramicrotome for in-resin-fluorescence detection in blocks, ribbons and sections on EM-grids...
October 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29051115/an-herbal-formula-consisting-of-schisandra-chinensis-turcz-baill-lycium-chinense-mill-and-eucommia-ulmoides-oliv-alleviates-disuse-muscle-atrophy-in-rats
#3
Seongguk Cho, Riwon Hong, Poorm Yim, Mijung Yeom, Bombi Lee, Woong Mo Yang, Jongki Hong, Hyang Sook Lee, Dae-Hyun Hahm
ETHNOPHARMACOLOGICAL RELEVANCE: Schisandra chinensis (Turcz.) Baill (SC), Lycium chinense Mill (LC) and Eucommia ulmoides Oliv (EU) are representative tonic herbal medicines that help to strengthen body muscles and bones making them stronger according to the Donguibogam, a tradition medical book of the Joseon Dynasty in Korea. AIM OF STUDY: To evaluate effects of an herbal formula consisting of SC, LC and EU on muscle atrophy in C2C12 myotubes and in a rat model of immobilization-induced muscle atrophy...
October 16, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29044624/effect-of-chronic-alcohol-abuse-on-anabolic-and-catabolic-signaling-pathways-in-human-skeletal-muscle
#4
Boris S Shenkman, Svetlana P Belova, Olga E Zinovyeva, Nudlya D Samkhaeva, Timur M Mirzoev, Natalia A Vilchinskaya, Erzhena G Altaeva, Olga V Turtikova, Tatiana Y Kostrominova, Tatiana L Nemirovskaya
BACKGROUND: Animal studies showed that alcoholic myopathy is characterized by the reduction of myofiber cross sectional area (CSA) and by impaired anabolic signaling. The goal of the current study was to compare changes in CSA and fiber type composition with modifications in anabolic and catabolic signaling pathways at the early stages of alcohol misuse in humans. METHODS: Skeletal muscle samples from seven male patients with chronic alcohol abuse (AL; 47.7±2.0 years old; alcohol misuse duration 7...
October 16, 2017: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/29044172/circulating-mir-19b-and-mir-181b-are-potential-biomarkers-for-diabetic-cardiomyopathy
#5
Camila Uribe Copier, Luis León, Mauricio Fernández, David Contador, Sebastián D Calligaris
Diabetic cardiomyopathy is characterized by metabolic changes in the myocardium that promote a slow and silent dysfunction of muscle fibers, leading to myocardium remodelling and heart failure, independently of the presence of coronary artery diseases or hypertension. At present, no imaging methods allow an early diagnosis of this disease. Circulating miRNAs in plasma have been proposed as biomarkers in the prognosis of several cardiac diseases. This study aimed to determine whether circulating miRNAs could be potential biomarkers of diabetic cardiomyopathy...
October 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29041497/anisotropic-light-propagation-in-bovine-muscle-tissue-depends-on-the-initial-fiber-orientation-muscle-type-and-wavelength
#6
Robbe Van Beers, Ben Aernouts, Marlon M Reis, Wouter Saeys
The effects of fiber orientation on vis/NIR light propagation were studied in three bovine muscles: biceps brachii, brachialis and soleus. Broadband light was focused onto the sample and the diffuse reflectance spot was captured using a hyperspectral camera (470-1620 nm), after which rhombuses were fitted to equi-intensity points. In samples with fibers running parallel to the measurement surface, the rhombus' major axis was oriented perpendicular to the fiber direction close to the point of illumination. However, at larger distances from the illumination spot, the major axis orientation aligned with the fiber direction...
September 4, 2017: Optics Express
https://www.readbyqxmd.com/read/29040534/macrophages-escape-klotho-gene-silencing-in-the-mdx-mouse-model-of-duchenne-muscular-dystrophy-and-promote-muscle-growth-and-increase-satellite-cell-numbers-through-a-klotho-mediated-pathway
#7
Michelle Wehling-Henricks, Steven S Welc, Guiseppina Samengo, Chiara Rinaldi, Catherine Lindsey, Ying Wang, Jeongyoon Lee, Makoto Kuro-O, James G Tidball
Duchenne muscular dystrophy (DMD) is a muscle wasting disease in which inflammation influences the severity of pathology. We found that the onset of muscle inflammation in the mdx mouse model of DMD coincides with large increases in expression of pro-inflammatory cytokines [tumor necrosis factor-α (TNFα); interferon gamma (IFNγ)] and dramatic reductions of the pro-myogenic protein Klotho in muscle cells and large increases of Klotho in pro-regenerative, CD206+ macrophages. Furthermore, TNFα and IFNγ treatments reduced Klotho in muscle cells and increased Klotho in macrophages...
October 12, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/29040321/co-delivery-of-micronized-urinary-bladder-matrix-damps-regenerative-capacity-of-minced-muscle-grafts-in-the-treatment-of-volumetric-muscle-loss-injuries
#8
Stephen M Goldman, Benjamin T Corona
Minced muscle grafts (MG) promote de novo muscle fiber regeneration and neuromuscular strength recovery in small and large animal models of volumetric muscle loss. The most noteworthy limitation of this approach is its reliance on a finite supply of donor tissue. To address this shortcoming, this study sought to evaluate micronized acellular urinary bladder matrix (UBM) as a scaffolding to promote in vivo expansion of this MG therapy in a rat model. Rats received volumetric muscle loss injuries to the tibialis anterior muscle of their left hind limb which were either left untreated or repaired with minced muscle graft at dosages of 50% and 100% of the defect mass, urinary bladder matrix in isolation, or a with an expansion product consisting of a combination of the two putative therapies in which the minced graft is delivered at a dosage of 50% of the defect mass...
2017: PloS One
https://www.readbyqxmd.com/read/29038428/denervation-related-alterations-and-biological-activity-of-mirnas-contained-in-exosomes-released-by-skeletal-muscle-fibers
#9
Rita De Gasperi, Sayyed Hamidi, Lauren M Harlow, Hanna Ksiezak-Reding, William A Bauman, Christopher P Cardozo
Exosomes are vesicles released by many eukaryotic cells; their cargo includes proteins, mRNA and microRNA (miR) that can be transferred to recipient cells and regulate cellular processes in an autocrine or paracrine manner. While cells of the myoblast lineage secrete exosomes, it is not known whether skeletal muscle fibers (myofibers) release exosomes. In this study, we found that cultured myofibers release nanovesicles that have bilamellar membranes and an average size of 60-130 nm, contain typical exosomal proteins and miRNAs and are taken up by C2C12 cells...
October 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29037825/vitamin-d-prevents-lipid-accumulation-in-murine-muscle-through-regulation-of-ppar%C3%AE-and-perilipin-2-expression
#10
Jiarong Li, Milton Mihalcioiu, Lifeng Li, Mahvash Zakikhani, Anne Camirand, Richard Kremer
Vitamin D plays an important role in regulation of skeletal muscle tone and contraction. Serum vitamin D status is linked to muscle power and force in adolescent girls, and vitamin D deficiency is associated with myopathies in children and poorer physical performance in the elderly. We previously reported that vitamin D deficiency is linked to a significant increase in muscle fatty infiltration in healthy young women, and studies in patients with neuromuscular disorders also associate muscle weakening and lipid content...
October 13, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29037386/influence-of-temperature-and-exercise-on-growth-performance-muscle-and-adipose-tissue-in-pacus-piaractus-mesopotamicus
#11
Vander Bruno Dos Santos, Marcelo Wendeborn Miranda de Oliveira, Rondinelle Artur Simões Salomão, Rosemeire de Souza Santos, Tassiana Gutierrez de Paula, Maeli Dal Pai Silva, Edson Assunção Mareco
The aim of this study was to evaluate the effects of temperature and swimming exercise on fish growth in pacus (Piaractus mesopotamicus). Pacus weighing 0.9 - 1.9g and 2.7 - 4.2cm in standard length were cultivated at an initial density of 120 fish m(-3) in 3 recirculation systems containing 6 water tanks at a volume of 0.5m(3) each at temperatures of 24, 28 and 32°C. At each temperature, three tanks were modified to generate exercise activity in the specimens and force the fish to swim under a current speed of 27...
October 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/29037169/left-ventricular-non-compaction-cardiomyopathy-with-coronary-artery-anomaly-complicated-by-ventricular-tachycardia
#12
Gustav Mattsson, Abdullah Baroudi, Hoshmand Tawfiq, Peter Magnusson
BACKGROUND: Non-compaction cardiomyopathy (NCCM) is characterized by prominent trabeculations, deep intertrabecular recesses, and a thick non-compacted endocardial myocardium. Prevalence in the general population remains unclear, but echocardiography series report 0.05%. During fetal development muscle fibers and trabeculae should compact into a solid myocardium and when this fails, NCCM occurs. The condition is genetic, even though acquired forms have been described. Worsening myocardial dysfunction may lead to heart failure and/or arrhythmias...
October 16, 2017: BMC Cardiovascular Disorders
https://www.readbyqxmd.com/read/29032593/association-analysis-of-the-actn3-r577x-polymorphism-with-passive-muscle-stiffness-and-muscle-strain-injury
#13
Naokazu Miyamoto, Eri Miyamoto-Mikami, Kosuke Hirata, Noriko Kimura, Noriyuki Fuku
Passive muscle stiffness is considered to be a major factor affecting joint flexibility and is thought to relate to the occurrence of muscle strain injury. In skinned muscle fiber experiments, the R577X polymorphism of the α-actinin-3 gene (ACTN3) has been associated with passive muscle stiffness. Our primary purpose was to clarify whether the ACTN3 R577X polymorphism influences passive stiffness of human muscle in vivo. We also examined whether the ACTN3 R577X polymorphism is associated with the occurrence of hamstring strain injury...
October 15, 2017: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/29031337/physiologic-changes-of-the-musculoskeletal-system-with-aging-a-brief-review
#14
REVIEW
Walter R Frontera
Aging is one of the important challenges of modern society. Advanced adult age is associated with changes in many physiologic systems. Of particular interest is the musculoskeletal system because it directly contributes to mobility and functional independence. Skeletal muscle mass and strength decline with age. These changes are mostly due to a reduction in the number of muscle fibers and cellular and molecular changes that reduce the force-generation process. Bone mass and architecture are compromised and may result in fractures...
November 2017: Physical Medicine and Rehabilitation Clinics of North America
https://www.readbyqxmd.com/read/29031330/histological-changes-in-the-proximal-suspensory-ligament-after-neurectomy-of-the-deep-branch-of-the-lateral-palmar-nerve-of-horses-with-induced-proximal-suspensory-desmitis
#15
Gabriela Lopez-Navarro, Francisco J Trigo-Tavera, Alejandro Rodriguez-Monterde, Gabriel Gutierrez-Ospina, Miguel Arechavaleta-Velasco, John Schumacher, James Schumacher
Proximal suspensory desmitis (PSD) is a common cause of lameness in the pelvic limb, but could also affect the thoracic limb of competing and non-competing horses. Most horses diagnosed with PSD in a thoracic limb respond to rest followed by controlled exercise, but in a small percentage of affected horses, lameness persists. In one study, four horses chronically lame because of PSD in a thoracic limb became sound after neurectomy of the deep branch of the lateral palmar nerve (DBLPaN), which innervates the proximal aspect of the suspensory ligament (SL; Guasco et al...
September 2017: Veterinary Journal
https://www.readbyqxmd.com/read/29024874/electrical-impedance-myography-changes-after-incomplete-cervical-spinal-cord-injury-an-examination-of-hand-muscles
#16
Le Li, Henry Shin, Argyrios Stampas, Xiaoyan Li, Ping Zhou
OBJECTIVE: This study was to apply the newly developed electrical impedance myography (EIM) technique to examine hand muscles in patients with an incomplete cervical spinal cord injury (SCI). METHODS: EIM was performed on the thenar, hypothenar, and first dorsal interosseous (FDI) muscles of SCI (n=16) and age-matched healthy control (n=18) subjects. By sending low intensity and high frequency current through the skin and measuring the consequent voltage, EIM estimates the major impedance parameters, which include resistance (R), reactance (X) and phase angle (θ)...
September 20, 2017: Clinical Neurophysiology: Official Journal of the International Federation of Clinical Neurophysiology
https://www.readbyqxmd.com/read/29023221/evaluation-of-the-effect-of-phototherapy-in-patients-with-mandibular-fracture-on-mandibular-dynamics-pain-edema-and-bite-force-a-pilot-study
#17
Leandro Lauriti, João Gualberto de Cerqueira Luz, Raquel Agnelli Mesquita-Ferrari, Kristianne Porta Santos Fernandes, Alessandro Melo Deana, Anna Carolina Ratto Tempestini Horliana, Larissa Costa-Santos, Aldo Brugnera Junior, Sandra Kalil Bussadori
OBJECTIVE: The aim of this study was to evaluate the effect of phototherapy on bite force, facial swelling, mandibular movements, and pain in patients having undergone surgical treatment for mandibular fractures. BACKGROUND: These are among the predominant types of facial fractures, and treatment involving surgical fixation with titanium plates is one of the most common procedures in oral-maxillofacial surgery. Phototherapy has been used to accelerate the muscle healing process and significantly improves muscle regeneration by inducing the formation of new muscle fibers...
October 12, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/29023009/myogenic-satellite-cells-biological-milieu-and-possible-clinical-applications
#18
Raed S Said, Ayman G Mustafa, Hasan A Asfour, Emad I Shaqoura
Adult skeletal muscle is a post-mitotic terminally differentiated tissue that possesses an immense potential for regeneration after injury. This regeneration can be achieved by adult stem cells named satellite cells that inhabit the muscular tissue. These cells were first identified in 1961 and were described as being wedged between the plasma membrane of the muscle fiber and the surrounding basement membrane. Since their discovery, many researchers investigated their embryological origin and the exact role they play in muscle regeneration and repair...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29021362/chloride-channels-take-center-stage-in-acute-regulation-of-excitability-in-skeletal-muscle-implications-for-fatigue
#19
REVIEW
Ole Bækgaard Nielsen, Frank Vincenzo de Paoli, Anders Riisager, Thomas Holm Pedersen
Initiation and propagation of action potentials in muscle fibers is a key element in the transmission of activating motor input from the central nervous system to their contractile apparatus, and maintenance of excitability is therefore paramount for their endurance during work. Here, we review current knowledge about the acute regulation of ClC-1 channels in active muscles and its importance for muscle excitability, function, and fatigue.
November 2017: Physiology
https://www.readbyqxmd.com/read/29019587/end-to-side-versus-end-to-end-neurorrhaphy-at-the-peroneal-nerve-in-rats
#20
Fausto Viterbo, Ryane Schmidt Brock, Fábio Maciel, Benoit Ayestaray, José Antonio Garbino, Cecília Pessoa Rodrigues
PURPOSE: To evaluate three different kinds of neurorrhaphy of the peroneal nerve. METHODS: Eigthy rats were divided into 5 groups. Control: nerve had no intervention. End-to-end (EE): nerve was cut and elongated with a nerve graft with two end-to-end neurorrhaphies. End-to-side (ES): nerve was cut and sutured to the graft with at the lateral side of the nerve. Side-to-end (SE): the nerve was cut and sutured to the graft with end-to-end neurorrhaphy. Denervated: nerve was cut and both endings were buried into the muscle...
September 2017: Acta Cirúrgica Brasileira
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