Tyler S Alioto, Marta Gut, Bruno Kotska Rodiño-Janeiro, Fernando Cruz, Jèssica Gómez-Garrido, Juan Carlos Vázquez-Ucha, Caterina Mata, Regina Antoni, Ferran Briansó, Marc Dabad, Eloi Casals, Matthew Ingham, Miguel Álvarez-Tejado, Germán Bou, Ivo G Gut
In response to the threat of increasing antimicrobial resistance, we must increase the amount of available high-quality genomic data gathered on antibiotic-resistant bacteria. To this end, we developed an integrated pipeline for high-throughput long-read sequencing, assembly, annotation and analysis of bacterial isolates and used it to generate a large genomic data set of carbapenemase-producing Enterobacterales (CPE) isolates collected in Spain. The set of 461 isolates were sequenced with a combination of both Illumina and Oxford Nanopore Technologies (ONT) DNA sequencing technologies in order to provide genomic context for chromosomal loci and, most importantly, structural resolution of plasmids, important determinants for transmission of antimicrobial resistance...
November 2023: Microbial Genomics