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Bone marrow microcirculation

Laibaik Park, Ken Uekawa, Lidia Garcia-Bonilla, Kenzo Koizumi, Michelle Murphy, Rose Pitstick, Linda H Younkin, Steven G Younkin, Ping Zhou, Geroge A Carlson, Josef Anrather, Costantino Iadecola
Rationale: Increasing evidence indicates that alterations of the cerebral microcirculation may play a role in Alzheimer's disease (AD), the leading cause of late-life dementia. The amyloid-β peptide (Aβ), a key pathogenic factor in AD, induces profound alterations in neurovascular regulation through the innate immunity receptor CD36, which, in turn, activates a Nox2-containing NADPH oxidase leading to cerebrovascular oxidative stress. Brain perivascular macrophages (PVM) located in the perivascular space, a major site of brain Aβ collection and clearance, are juxtaposed to the wall of intracerebral resistance vessels and are a powerful source of reactive oxygen species (ROS)...
May 17, 2017: Circulation Research
Emma Lefrançais, Guadalupe Ortiz-Muñoz, Axelle Caudrillier, Beñat Mallavia, Fengchun Liu, David M Sayah, Emily E Thornton, Mark B Headley, Tovo David, Shaun R Coughlin, Matthew F Krummel, Andrew D Leavitt, Emmanuelle Passegué, Mark R Looney
Platelets are critical for haemostasis, thrombosis, and inflammatory responses, but the events that lead to mature platelet production remain incompletely understood. The bone marrow has been proposed to be a major site of platelet production, although there is indirect evidence that the lungs might also contribute to platelet biogenesis. Here, by directly imaging the lung microcirculation in mice, we show that a large number of megakaryocytes circulate through the lungs, where they dynamically release platelets...
April 6, 2017: Nature
XianFeng Wang, Nancy L Buechler, Barbara K Yoza, Charles E McCall, Vidula Vachharajani
BACKGROUND: Morbid obesity increases the cost of care in critically ill patients. Sepsis is the leading cause of death in noncoronary intensive care units. Circulating cell-endothelial cell interactions in microcirculation are the rate-determining factors in any inflammation; obesity increases these interactions further. Adiponectin deficiency is implicated in increased cardiovascular risk in obese patients. We have shown that adiponectin deficiency increases microvascular dysfunction in early sepsis...
2016: Journal of Inflammation Research
E Peled, M Assalia, L Axelman, D Norman, Y Nadir
INTRODUCTION: The blood supply of the bone and bone marrow are interconnected through a network of vessels. Arteries penetrating the bone flow into the marrow cavity while blood from marrow sinuses and veins leave the tissue via the bone. AIM: The current study was designed to search for thrombosis in the small blood vessels of bone and bone marrow. MATERIALS AND METHODS: Bone biopsies of 40 patients were studied using specific staining to fibrin with martin scarlet blue (MSB) and by immune-staining to tissue factor (TF), TF pathway inhibitor and heparanase that is a pro-angiogenic and pro-coagulant protein...
April 2016: Thrombosis Research
Jens Hillengass, Judith Ritsch, Maximilian Merz, Barbara Wagner, Christina Kunz, Thomas Hielscher, Hendrik Laue, Tobias Bäuerle, Christian M Zechmann, Anthony D Ho, Heinz-Peter Schlemmer, Hartmut Goldschmidt, Thomas M Moehler, Stefan Delorme
This prospective study aimed to investigate the prognostic significance of dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) as a non-invasive imaging technique delivering the quantitative parameters amplitude A (reflecting blood volume) and exchange rate constant kep (reflecting vascular permeability) in patients with asymptomatic monoclonal plasma cell diseases. We analysed DCE-MRI parameters in 33 healthy controls and 148 patients with monoclonal gammopathy of undetermined significance (MGUS) or smouldering multiple myeloma (SMM) according to the 2003 IMWG guidelines...
July 2016: British Journal of Haematology
Marcelo Franchin, David F Cólon, Fernanda V S Castanheira, Marcos G da Cunha, Bruno Bueno-Silva, Severino M Alencar, Thiago M Cunha, Pedro L Rosalen
Vestitol is an isoflavonoid isolated from Brazilian red propolis with potential anti-inflammatory activity. This study investigated the mechanism of action of vestitol on the modulation of neutrophil migration in the inflammatory process. Pre-treatment with vestitol at 1, 3, or 10 mg/kg reduced LPS- or mBSA-induced neutrophil migration and the release of CXCL1/KC and CXCL2/MIP-2 in vivo. Likewise, pre-treatment with vestitol at 1, 3, or 10 μM reduced the levels of CXCL1/KC and CXCL2/MIP-2 in macrophage supernatants in vitro...
April 22, 2016: Journal of Natural Products
Gen Takagi, Masaaki Miyamoto, Yoshimitsu Fukushima, Masahiro Yasutake, Shuhei Tara, Ikuyo Takagi, Naoki Seki, Shinichiro Kumita, Wataru Shimizu
UNLABELLED: One problem of vascular angiogenesis therapy is the lack of reliable methods for evaluating blood flow in the microcirculation. We aimed to assess whether (99m)Tc-macroaggregated albumin perfusion scintigraphy ((99m)Tc-MAA) predicts quantitated blood flow after therapeutic angiogenesis in patients with peripheral artery disease. METHODS: Forty-six patients with peripheral artery disease were treated with bone marrow mononuclear cell implantation (BMCI)...
February 2016: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Dominga Lapi, Sabrina Vagnani, Daniela Sapio, Teresa Mastantuono, Francesca Boscia, Giuseppe Pignataro, Claudia Penna, Pasquale Pagliaro, Antonio Colantuoni
Previous studies have shown that the pial microcirculation remodeling improves neurological outcome after middle cerebral artery occlusion (MCAO), accompanied by higher expression of vascular endothelial growth factor (VEGF) and endothelial nitric oxide synthase (eNOS), modulating in vivo angiogenesis. This study was aimed to assess the effects of bone marrow mesenchymal stem cells (BM-MSCs) infused after MCAO on rat pial microcirculation. Animals were subjected to 2 h MCAO followed by BM-MSCs infusion into internal carotid artery...
2015: Frontiers in Cellular Neuroscience
Kamil Bujak, Jarosław Wasilewski, Tadeusz Osadnik, Sandra Jonczyk, Aleksandra Kołodziejska, Marek Gierlotka, Mariusz Gąsior
Red blood cell distribution width (RDW) is a measure of red blood cell volume variations (anisocytosis) and is reported as part of a standard complete blood count. In recent years, numerous studies have noted the importance of RDW as a predictor of poor clinical outcomes in the settings of various diseases, including coronary artery disease (CAD). In this paper, we discuss the prognostic value of RDW in CAD and describe the pathophysiological connection between RDW and acute coronary syndrome. In our opinion, the negative prognostic effects of elevated RDW levels may be attributed to the adverse effects of independent risk factors such as inflammation, oxidative stress, and vitamin D3 and iron deficiency on bone marrow function (erythropoiesis)...
2015: Disease Markers
Dipanjan Pan, Christine T N Pham, Katherine N Weilbaecher, Michael H Tomasson, Samuel A Wickline, Gregory M Lanza
Sn2 lipase labile phospholipid prodrugs in conjunction with contact-facilitated drug delivery offer an important advancement in Nanomedicine. Many drugs incorporated into nanosystems, targeted or not, are substantially lost during circulation to the target. However, favorably altering the pharmacokinetics and volume of distribution of systemic drug delivery can offer greater efficacy with lower toxicity, leading to new prolonged-release nanoexcipients. However, the concept of achieving Paul Erhlich's inspired vision of a 'magic bullet' to treat disease has been largely unrealized due to unstable nanomedicines, nanosystems achieving low drug delivery to target cells, poor intracellular bioavailability of endocytosed nanoparticle payloads, and the substantial biological barriers of extravascular particle penetration into pathological sites...
January 2016: Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology
Maximilian Merz, Thomas M Moehler, Judith Ritsch, Tobias Bäuerle, Christian M Zechmann, Barbara Wagner, Anna Jauch, Dirk Hose, Christina Kunz, Thomas Hielscher, Hendrik Laue, Hartmut Goldschmidt, Stefan Delorme, Jens Hillengass
OBJECTIVES: Aim of this prospective study was to investigate prognostic significance of increased bone marrow microcirculation as detected by dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) for survival and local complications in patients with multiple myeloma (MM). METHODS: We performed DCE-MRI of the lumbar spine in 131 patients with newly diagnosed MM and analysed data according to the Brix model to acquire amplitude A and exchange rate constant kep...
May 2016: European Radiology
Jan Skóra, Artur Pupka, Dariusz Janczak, Piotr Barć, Tomasz Dawiskiba, Krzysztof Korta, Dagmara Baczyńska, Agnieszka Mastalerz-Migas, Jerzy Garcarek
INTRODUCTION: Our study was designed to investigate the safety and efficacy of combined autologous bone marrow mononuclear cell (MNC) and gene therapy in comparison to conventional drug therapy in patients with critical limb ischemia (CLI). MATERIAL AND METHODS: Thirty-two patients with CLI persisting for 12-48 months (average time 27.5 months) were randomized into 2 groups, each group consisting of 16 patients. In the first group, administration of autologous bone marrow MNC and vascular endothelial growth factor (VEGF) plasmid was performed...
April 25, 2015: Archives of Medical Science: AMS
Yonggang Pang, Olga Tsigkou, Joel A Spencer, Charles P Lin, Craig Neville, Brian Grottkau
Vascularization is a key challenge in tissue engineering. Three-dimensional structure and microcirculation are two fundamental parameters for evaluating vascularization. Microscopic techniques with cellular level resolution, fast continuous observation, and robust 3D postimage processing are essential for evaluation, but have not been applied previously because of technical difficulties. In this study, we report novel video-rate confocal microscopy and 3D postimage processing techniques to accomplish this goal...
October 2015: Tissue Engineering. Part C, Methods
Lin Ou-Yang, Guang-ming Lu
STUDY DESIGN: Descriptive study, stratified sampling. OBJECTIVE: Using dynamic computed tomographic perfusion (CTP) to explore the age-related distribution patterns of the microcirculation perfusion in the vertebral marrow, the vertebral bone mineral density (BMD), and the intervertebral discal degeneration (IDD) further to discuss the possible causation between them. SUMMARY OF BACKGROUND DATA: A latest viewpoint deemed that reduced blood supply of the vertebral marrow was correlated with an increased incidence of IDD and loss of BMD...
May 15, 2015: Spine
B Meehan, A Dombrovsky, N Magnus, J Rak
The critical role of the vasculature in cancer progression is predominantly studied at the capillary level, and often equated with angiogenesis. However, the mechanisms that ensure the supply of increasing blood volume to the expanding tumor microcirculation remain presently unclear. Here we used established mouse tumor models to document the enlargement (arteriogenesis), of macroscopic feeding vessels at considerable distances upstream from the malignant lesion, but not contralaterally. These changes are not affected by the procoagulant host tissue factor (TF), but are modulated by vascular ageing and atherosclerosis in ApoE-/- mice...
2015: Neoplasma
Andrea Zanetti, Michelle Grata, Emily B Etling, Regeant Panday, Flordeliza S Villanueva, Catalin Toma
Mesenchymal stem cells (MSCs) have been extensively explored in a variety of regenerative medicine applications. The relatively large size of MSCs expanded in tissue culture flasks leads to retention in the microcirculation of the lungs following intravenous delivery, reducing their capacity to reach target sites. We explored whether the expansion of whole marrow in suspension cultures would yield smaller MSCs with increased capacity to traverse the pulmonary microcirculation compared with traditional monolayer cultures...
July 2015: Tissue Engineering. Part C, Methods
Rintaro Nishimura, Tetsu Nishiwaki, Takeshi Kawasaki, Ayumi Sekine, Rika Suda, Takashi Urushibara, Toshio Suzuki, Shin Takayanagi, Jiro Terada, Seiichiro Sakao, Koichiro Tatsumi
Exposure to hypoxia induces changes in the structure and functional phenotypes of the cells composing the pulmonary vascular wall from larger to most peripheral vessels. Endothelial progenitor cells (EPCs) may be involved in vascular endothelial repair. Resident EPCs with a high proliferative potential are found in the pulmonary microcirculation. However, their potential location, identification, and functional role have not been clearly established. We investigated whether resident EPCs or bone marrow (BM)-derived EPCs play a major role in hypoxic response of pulmonary vascular endothelial cells (PVECs)...
April 15, 2015: American Journal of Physiology. Lung Cellular and Molecular Physiology
Hii-Sun Jeong, Hye-Kyung Lee, Kwan-Chul Tark, Dae-Hyun Lew, Yoon-Woo Koh, Chul-Hoon Kim, In-Suck Seo
The purpose of this study was to devise an expanded ischemic flap model and to investigate the role of AMD-3100 (Plerixafor, chemokine receptor 4 inhibitor) in this model by confirming its effect on mobilization of stem cells from the bone marrow. Male Sprague-Dawley rats were used as an animal research model. The mobilization of stem cells from the bone marrow was confirmed in the AMD-3100-treated group. The fractions of endothelial progenitor cells (EPC) and the vascular endothelial growth factor receptor (VEGFR) 2+ cells in the peripheral blood were increased in groups treated with AMD-3100...
November 2014: Journal of Korean Medical Science
Maximilian Merz, Judith Ritsch, Christina Kunz, Barbara Wagner, Sandra Sauer, Dirk Hose, Thomas Moehler, Stefan Delorme, Hartmut Goldschmidt, Christian Zechmann, Jens Hillengass
PURPOSE: To noninvasively assess bone marrow microcirculation before and after therapy in patients with newly diagnosed multiple myeloma with dynamic contrast-enhanced MRI (DCE-MRI). EXPERIMENTAL DESIGN: Ninety-six patients received DCE-MRI before and after primary treatment for newly diagnosed multiple myeloma. For the 91 evaluable patients, treatment consisted of high-dose therapy (HDT) with autologous stem cell transplantation (ASCT) in 82 patients and chemotherapy without ASCT in 9 patients...
January 1, 2015: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Yi-Xian Qin, Minyi Hu
While mechanotransductive signal is proven essential for tissue regeneration, it is critical to determine specific cellular responses to such mechanical signals and the underlying mechanism. Dynamic fluid flow induced by mechanical loading has been shown to have the potential to regulate bone adaptation and mitigate bone loss. Mechanotransduction pathways are of great interests in elucidating how mechanical signals produce such observed effects, including reduced bone loss, increased bone formation, and osteogenic cell differentiation...
2014: BioMed Research International
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