keyword
MENU ▼
Read by QxMD icon Read
search

Functional morphology

keyword
https://www.readbyqxmd.com/read/29235444/contact-guidance-for-cardiac-tissue-engineering-using-3d-bioprinted-gelatin-patterned-hydrogel
#1
Ajay Tijore, Scott Alexander Irvine, Udi Sarig, Priyadarshini Mhaisalkar, Vrushali Baisane, Subbu S Venkatraman
Here, we have developed a 3D bioprinted microchanneled gelatin hydrogel that promotes human mesenchymal stem cell (hMSC) myocardial commitment and supports native cardiomyocytes (CMs) contractile functionality. Firstly, we studied the effect of bioprinted microchanneled hydrogel on the alignment, elongation, and differentiation of hMSC. Notably, the cells displayed well defined F-actin anisotropy and elongated morphology on the microchanneled hydrogel, hence showing the effects of topographical control over cell behavior...
December 13, 2017: Biofabrication
https://www.readbyqxmd.com/read/29235216/surface-molding-of-microscale-hydrogels-with-microactuation-functionality
#2
John J Bowen, Mark A Rose, Abhiteja Konda, Stephen A Morin
This work describes the fabrication of numerous hydrogel microstructures, μ-gels, via a process called "surface molding"-chemically patterned elastomeric assembly substrates were used to organize and manipulate the geometry of liquid prepolymer microdroplets, which, following photo-initiated crosslinking, maintained the desired morphology. By adjusting the state of strain during the crosslinking process, a continua of structures could be created using one pattern. These arrays of μ-gels have stimuli-responsive properties that are directly applicable to actuation where the basis shape and array geometry of the μ-gels can be used to rationally generate microactuators with programmed motions...
December 12, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29235182/spinal-cord-injury-induced-cardiomyocyte-atrophy-and-impaired-cardiac-function-are-severity-dependent
#3
Jordan W Squair, Jie Liu, Wolfram Tetzlaff, Andrei V Krassioukov, Christopher R West
The principle aim of the present study was to assess how the severity of spinal cord injury (SCI) affects LV mechanics, function, and underlying cardiomyocyte morphology. Here, we utilized different severities of T3 spinal cord contusions (MODERATE, 200Kdyn contusion; SEVERE, 400kdyn contusion; SHAM) and combined standard echocardiography with speckle tracking analyses to investigate in vivo cardiac function and deformation (contractility) after experimental SCI in the Wistar rat. In addition, we investigated changes in the intrinsic structure of cardiac myocytes ex vivo...
December 12, 2017: Experimental Physiology
https://www.readbyqxmd.com/read/29235100/embryonic-exposure-to-valproic-acid-affects-the-histaminergic-system-and-the-social-behavior-of-adult-zebrafish-danio-rerio
#4
Diego Baronio, Henri A J Puttonen, Maria Sundvik, Svetlana Semenova, Essi Lehtonen, Pertti Panula
BACKGROUND AND PURPOSE: Histamine modulates several behaviors and physiological functions and its deficiency is associated with neuropsychiatric disorders. Gestational intake of valproic acid (VPA) is linked to Autism Spectrum Disorder (ASD), which is characterized by impaired sociability and stereotypies. VPA effects on the neurochemistry and functional morphology of the histaminergic system in ASD are unclear. Zebrafish are highly social and given the similarities between zebrafish and human neurotransmitter systems, we decided to use zebrafish to study VPA effects on histamine...
December 12, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29234951/zinc-finger-transcription-factor-odd-skipped-related-1-regulates-cranial-bone-formation
#5
Shinji Kawai, Masashi Yamauchi, Atsuo Amano
Knowledge of the molecular mechanisms of bone formation has been advanced by novel findings related to genetic control. Odd-skipped related 1 (Osr1) is known to play important roles in embryonic, heart, and urogenital development. To elucidate the in vivo function of Osr1 in bone formation, we generated transgenic mice overexpressing full-length Osr1 under control of its 2.8-kb promoter, which were smaller than their wild-type littermates. Notably, abnormalities in the skull of Osr1 transgenic mice were revealed by analysis of X-ray, skeletal preparation, and morphological findings, including round skull and cranial dysraphism...
December 12, 2017: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/29234889/translaminar-circuits-formed-by-the-pyramidal-cells-in-the-superficial-layers-of-cat-visual-cortex
#6
German Koestinger, Kevan A C Martin, Elisha S Rusch
Pyramidal cells in the superficial layers of the neocortex provide a major excitatory projection to layer 5, which contains the pyramidal cells that project to subcortical motor-related targets. Both structurally and functionally rather little is known about this interlaminar pathway, especially in higher mammals. Here, we made sparse ultrastructural reconstructions of the projection to layer 5 of three pyramidal neurons from layer 3 in cat V1 whose morphology, physiology, and synaptic connections with layers 2 and 3 were known...
December 12, 2017: Brain Structure & Function
https://www.readbyqxmd.com/read/29234687/delivery-of-cr2-fh-using-aav-vector-therapy-as-treatment-strategy-in-the-mouse-model-of-choroidal-neovascularization
#7
Gloriane Schnabolk, Nathaniel Parsons, Elisabeth Obert, Balasubramaniam Annamalai, Cecile Nasarre, Stephen Tomlinson, Alfred S Lewin, Bärbel Rohrer
Complement activation plays a significant role in age-related macular degeneration (AMD) pathogenesis, and polymorphisms interfering with factor H (fH) function, a complement alternative pathway (AP) inhibitor, are associated with increased AMD risk. We have previously validated an AP inhibitor, a fusion protein consisting of a complement receptor 2 fragment linked to the inhibitory domain of fH (CR2-fH) as an efficacious treatment for choroidal neovascularization (CNV) when delivered intravenously. Here we tested an alternative approach of AAV-mediated delivery (AAV5-VMD2-CR2-fH or AAV5-VMD2-mCherry) using subretinal delivery in C57BL/6J mice...
June 15, 2018: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/29234492/no-preliminary-evidence-of-differences-in-astrocyte-density-within-the-white-matter-of-the-dorsolateral-prefrontal-cortex-in-autism
#8
Ting Ting Lee, Efstratios Skafidas, Mirella Dottori, Daniela Zantomio, Christos Pantelis, Ian Everall, Gursharan Chana
Background: While evidence for white matter and astrocytic abnormalities exist in autism, a detailed investigation of astrocytes has not been conducted. Such an investigation is further warranted by an increasing role for neuroinflammation in autism pathogenesis, with astrocytes being key players in this process. We present the first study of astrocyte density and morphology within the white matter of the dorsolateral prefrontal cortex (DLPFC) in individuals with autism. Methods: DLPFC formalin-fixed sections containing white matter from individuals with autism (n = 8, age = 4-51 years) and age-matched controls (n = 7, age = 4-46 years) were immunostained for glial fibrillary acidic protein (GFAP)...
2017: Molecular Autism
https://www.readbyqxmd.com/read/29234407/jinhong-tablet-reduces-damage-of-intestinal-mucosal-barrier-in-rats-with-acute-biliary-infection-via-bcl-2-bax-mrna-and-protein-regulation
#9
Xiaoqiang Liang, Xiao Ni, YongQi Wang, Jinkun Xie, Xuelin Zhang, Honggang Gu, Jingzhe Zhang
Objective: To explore the effects and mechanism of Jinhong Tablet on intestinal mucosal barrier function and SIRS in rats with acute biliary infection. Methods: 36 SD male rats were divided into three groups: sham operation (control), acute biliary infection (ABI) model, and Jinhong Tablet (Jinhong) group. Jinhong group were force-fed with Jinhong Tablet, while the other two groups received oral saline. At days 3 and 5, morphological changes of intestinal mucosa were assessed...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/29234347/ectopic-expression-of-pumpkin-nac-transcription-factor-cmnac1-improves-multiple-abiotic-stress-tolerance-in-arabidopsis
#10
Haishun Cao, Li Wang, Muhammad A Nawaz, Mengliang Niu, Jingyu Sun, Junjun Xie, Qiusheng Kong, Yuan Huang, Fei Cheng, Zhilong Bie
Drought, cold and salinity are the major environmental stresses that limit agricultural productivity. NAC transcription factors regulate the stress response in plants. Pumpkin (Cucurbita moschata) is an important cucurbit vegetable crop and it has strong resistance to abiotic stress; however, the biological functions of stress-related NAC genes in this crop are largely unknown. This study reports the function of CmNAC1, a stress-responsive pumpkin NAC domain protein. The CmNAC1-GFP fusion protein was transiently expressed in tobacco leaves for subcellular localization analysis, and we found that CmNAC1 is localized in the nucleus...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29234165/short-physical-performance-battery-for-cardiovascular-disease-inpatients-implications-for-critical-factors-and-sarcopenia
#11
Tomohiro Yasuda, Toshiaki Nakajima, Tatsuya Sawaguchi, Naohiro Nozawa, Tomoe Arakawa, Reiko Takahashi, Yuta Mizushima, Satoshi Katayanagi, Kazuhisa Matsumoto, Shigeru Toyoda, Teruo Inoue
We examined the relationship between Short Physical Performance Battery (SPPB) and clinical and laboratory factors and the effect of sarcopenia and sarcopenic obesity (SO) on clinical and laboratory factors for cardiovascular disease (CVD) inpatients. CVD male (n = 318) and female (n = 172) inpatients were recruited. A stepwise multiple-regression analysis was performed to predict total SPPB scores and assess clinical and laboratory factors (physical characteristics, functional and morphological assessments, etc...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29234131/a-gene-expression-study-of-ornamental-fin-shape-in-neolamprologus-brichardi-an-african-cichlid-species
#12
Ehsan Pashay Ahi, Florian Richter, Kristina M Sefc
The diversity of fin morphology within and across fish taxa offers great, but still largely unexplored, opportunities to investigate the proximate mechanisms underlying fin shape variation. Relying on available genetic knowledge brought forth mainly by the comprehensive study of the zebrafish caudal fin, we explored candidate molecular mechanisms for the maintenance and formation of the conspicuously elongated filaments adorning the unpaired fins of the East African "princess cichlid" Neolamprologus brichardi...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29234072/biochemical-and-transcriptomic-analyses-of-drought-stress-responses-of-ly1306-tobacco-strain
#13
Huijuan Yang, Li Zhao, Shimin Zhao, Jing Wang, Hongzhi Shi
This study aimed to investigate drought resistance of the LY1306 tobacco strain. Seedlings of tobacco strains LY1306, ZhongYan 100 (ZY100) and Hong Hua Da Jin Yuan (HHDJY) were treated with polyethylene glycol (PEG)-6000 to induce osmotic stress. As validation, water-deficit-induced drought treatments, including mild drought (MD; watering 1.5 L/week) and severe drought (SD, without watering) were carried out. Changes in cell morphology, leaf water potential, antioxidant enzyme activity, as well as contents of malondialdehyde (MDA) and proline were determined for each treatment...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29233887/st6gal-i-sialyltransferase-promotes-tumor-necrosis-factor-tnf-mediated-cancer-cell-survival-via-sialylation-of-the-tnf-receptor-1-tnfr1-death-receptor
#14
Andrew T Holdbrooks, Colleen M Britain, Susan L Bellis
Activation of the TNFR1 death receptor by TNF induces either cell survival or cell death. However, the mechanisms mediating these distinct outcomes remain poorly understood. In the present study, we report that the ST6Gal-I sialyltransferase, an enzyme upregulated in numerous cancers, sialylates TNFR1, and thereby protects tumor cells from TNF-induced apoptosis. Using pancreatic and ovarian cancer cells with ST6Gal-I knockdown or overexpression, we determined that α2-6 sialylation of TNFR1 had no effect on early TNF-induced signaling events including the rapid activation of NFκB, JNK, ERK, and Akt (occurring within 15m)...
December 12, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29233811/dissecting-the-roles-of-the-calcineurin-pathway-in-unisexual-reproduction-stress-responses-and-virulence-in-cryptococcus-deneoformans
#15
Ci Fu, Nicholas Donadio, Maria E Cardenas, Joseph Heitman
The Ca2+/calmodulin-dependent protein phosphatase calcineurin orchestrates sexual reproduction, stress responses, and virulence via branched downstream pathways in the opportunistic human fungal pathogen, Cryptococcus neoformans The calcineurin binding protein Cbp1, the calcineurin temperature suppressor Cts1, the calcineurin responsive zinc finger transcription factor Crz1, and the calcineurin targets Pbp1, Tif3, and Puf4 all function downstream of calcineurin to orchestrate distinct cellular processes. To elucidate how the calcineurin pathway regulatory network governs unisexual reproduction, stress responses, and virulence, we have analyzed the self-filamentous C...
December 12, 2017: Genetics
https://www.readbyqxmd.com/read/29233778/phenotypic-indications-of-ftsz-inhibition-in-hok-sok-induced-bacterial-growth-changes-and-stress-response
#16
ChinweU Chukwudi, Liam Good
The hok/sok locus has been shown to enhance the growth of bacteria in adverse growth conditions such as high temperature, low starting-culture densities and antibiotic treatment. This is in addition to their well-established plasmid-stabilization effect via post-segregational killing of plasmid-free daughter cells. It delays the onset of growth by prolonging the lag phase of bacterial culture, and increases the rate of exponential growth when growth eventually begins. This enables the cells adapt to the prevailing growth conditions and enhance their survival in stressful conditions...
December 9, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29233554/accelerated-and-improved-differentiation-of-retinal-organoids-from-pluripotent-stem-cells-in-rotating-wall-vessel-bioreactors
#17
Tyler DiStefano, Holly Yu Chen, Christopher Panebianco, Koray Dogan Kaya, Matthew J Brooks, Linn Gieser, Nicole Y Morgan, Tom Pohida, Anand Swaroop
Pluripotent stem cells can be differentiated into 3D retinal organoids, with major cell types self-patterning into a polarized, laminated architecture. In static cultures, organoid development may be hindered by limitations in diffusion of oxygen and nutrients. Herein, we report a bioprocess using rotating-wall vessel (RWV) bioreactors to culture retinal organoids derived from mouse pluripotent stem cells. Organoids in RWV demonstrate enhanced proliferation, with well-defined morphology and improved differentiation of neurons including ganglion cells and S-cone photoreceptors...
December 1, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/29233476/e2f-dp-prevents-cell-cycle-progression-in-endocycling-fat-body-cells-by-suppressing-datm-expression
#18
Ana Guarner, Robert Morris, Michael Korenjak, Myriam Boukhali, Maria Paula Zappia, Capucine Van Rechem, Johnathan R Whetstine, Sridhar Ramaswamy, Lee Zou, Maxim V Frolov, Wilhelm Haas, Nicholas J Dyson
To understand the consequences of the complete elimination of E2F regulation, we profiled the proteome of Drosophila dDP mutants that lack functional E2F/DP complexes. The results uncovered changes in the larval fat body, a differentiated tissue that grows via endocycles. We report an unexpected mechanism of E2F/DP action that promotes quiescence in this tissue. In the fat body, dE2F/dDP limits cell-cycle progression by suppressing DNA damage responses. Loss of dDP upregulates dATM, allowing cells to sense and repair DNA damage and increasing replication of loci that are normally under-replicated in wild-type tissues...
December 1, 2017: Developmental Cell
https://www.readbyqxmd.com/read/29233188/the-rio-protein-kinase-encoding-gene-sj-riok-2-is-involved-in-key-reproductive-processes-in-schistosoma-japonicum
#19
Lu Zhao, Xin He, Christoph G Grevelding, Qing Ye, Ying Li, Robin B Gasser, Colette Dissous, Mudassar N Mughal, Yan-Qin Zhou, Jun-Long Zhao, Min Hu
BACKGROUND: Schistosomiasis is one of the most prevalent parasitic diseases worldwide and is caused by parasitic trematodes of the genus Schistosoma. The pathogenesis of schistosomiasis is caused by eggs whose production is the consequence of the pairing of schistosomes and the subsequent sexual maturation of the female. Previous studies have demonstrated that protein kinases are involved in processes leading to the male-induced differentiation of the female gonads, ovary and vitellarium...
December 12, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29232857/mir-34a-regulates-sperm-motility-in-zebrafish
#20
Wenjie Guo, Binyue Xie, Shuting Xiong, Xufang Liang, Jian-Fang Gui, Jie Mei
Increasing attention has been focused on the role of microRNAs in post-transcription regulation during spermatogenesis. Recently, the miR-34 family has been shown to be involved in the spermatogenesis, but the clear function of the miR-34 family in spermatogenesis is still obscure. Here we analyzed the function of miR-34a, a member of the miR-34 family, during spermatogenesis using miR-34a knockout zebrafish generated by the clustered regularly interspaced short palindromic repeats/associated protein 9 (CRISPR/Cas9) system...
December 10, 2017: International Journal of Molecular Sciences
keyword
keyword
49631
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"