Yue Deng, Yuqi Lin, Siyu Chen, Yuhang Xiang, Hongjia Chen, Shuyuan Qi, Hyung Suk Oh, Biswajit Das, Gloria Komazin-Meredith, Jean M Pesola, David M Knipe, Donald M Coen, Dongli Pan
Herpes simplex virus 1 (HSV-1) latent infection entails repression of viral lytic genes in neurons. By functional screening using luciferase-expressing HSV-1, we identify ten neuron-specific microRNAs potentially repressing HSV-1 neuronal replication. Transfection of miR-9, the most active candidate from the screen, decreases HSV-1 replication and gene expression in Neuro-2a cells. Ectopic expression of miR-9 from lentivirus or recombinant HSV-1 suppresses HSV-1 replication in male primary mouse neurons in culture and mouse trigeminal ganglia in vivo, and reactivation from latency in the primary neurons...
March 5, 2024: Nature Communications