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P H Zaidi, K Seetharam, Girish Krishna, L Krishnamurthy, S Gajanan, Raman Babu, M Zerka, M T Vinayan, B S Vivek
An association mapping panel, named as CIMMYT Asia association mapping (CAAM) panel, involving 396 diverse tropical maize lines were phenotyped for various structural and functional traits of roots under drought and well-watered conditions. The experiment was conducted during Kharif (summer-rainy) season of 2012 and 2013 in root phenotyping facility at CIMMYT-Hyderabad, India. The CAAM panel was genotyped to generate 955, 690 SNPs through GBS v2.7 using Illumina Hi-seq 2000/2500 at Institute for Genomic Diversity, Cornell University, Ithaca, NY, USA...
2016: PloS One
Ana Lúcia Anversa Segatto, Claudia Elizabeth Thompson, Loreta Brandão Freitas
Developmental genes are believed to contribute to major changes during plant evolution, from infrageneric to higher levels. Due to their putative high sequence conservation, developmental genes are rarely used as molecular markers, and few studies including these sequences at low taxonomic levels exist. WUSCHEL-related homeobox genes (WOX) are transcription factors exclusively present in plants and are involved in developmental processes. In this study, we characterized the infrageneric genetic variation of Petunia WOX genes...
October 20, 2016: Genetics and Molecular Biology
Delphine Mieulet, Sylvie Jolivet, Maud Rivard, Laurence Cromer, Aurore Vernet, Pauline Mayonove, Lucie Pereira, Gaëtan Droc, Brigitte Courtois, Emmanuel Guiderdoni, Raphael Mercier
Introduction of clonal reproduction through seeds (apomixis) in crops has the potential to revolutionize agriculture by allowing self-propagation of any elite variety, in particular F1 hybrids. In the sexual model plant Arabidopsis thaliana synthetic clonal reproduction through seeds can be artificially implemented by (i) combining three mutations to turn meiosis into mitosis (MiMe) and (ii) crossing the obtained clonal gametes with a line expressing modified CENH3 and whose genome is eliminated in the zygote...
October 21, 2016: Cell Research
Jenny Y Y Lau, Chun-Chiu Pang, Lawrence Ramsden, Richard M K Saunders
The floral phenology, pollination ecology and breeding systems of two sympatric early-divergent angiosperms, Goniothalamus tapisoides and G. suaveolens (Annonaceae) are compared. The flowers are protogynous and morphologically similar, with anthesis over 23-25 h. Both species are predominantly xenogamous and pollinated by small beetles: G. tapisoides mainly by Curculionidae and G. suaveolens mainly by Nitidulidae. Coevolution and reproductive resource partitioning, reducing interspecific pollen transfer, is achieved by temporal isolation, due to contrasting floral phenologies; and ethological isolation, due to contrasting floral scents that contain attractants specific to the two beetle families...
October 21, 2016: Scientific Reports
Abolfazl Aslani, Behzad Zolfaghari, Fatemeh Davoodvandi
Purpose: Recurrent aphthous stomatitis is a disease with unknown etiology that's mostly treated symptomatically and has no definite cure. Pomegranate (Punica granatum) flowers have been used as medicinal herb that due to its antimicrobial, antioxidant, anti-inflammatory, analgesic and healing effects, has been useful in treatment of oral aphthous. Therefore, we decided to formulate a mucoadhesive gel with pomegranate flower extract to reduce the need for corticosteroid therapy in patients. Methods: Pomegranate flowers are extracted by percolation method...
September 2016: Advanced Pharmaceutical Bulletin
Nancy Kokalis-Burelle, Erin N Rosskopf, David M Butler, Steven A Fennimore, John Holzinger
Steam and soil solarization were investigated for control of the root-knot nematode Meloidogyne arenaria in 2 yr of field trials on a commercial flower farm in Florida. The objective was to determine if preplant steam treatments in combination with solarization, or solarization alone effectively controlled nematodes compared to methyl bromide (MeBr). Trials were conducted in a field with naturally occurring populations of M. arenaria. Treatments were solarization alone, steam treatment after solarization using standard 7...
September 2016: Journal of Nematology
Ivana Generalić Mekinić, Ivica Blažević, Ivana Mudnić, Franko Burčul, Mia Grga, Danijela Skroza, Iva Jerčić, Ivica Ljubenkov, Mladen Boban, Mladen Miloš, Višnja Katalinić
Sea fennel, a rediscovered star of the coastal cuisine, has been investigated for its phytochemical profile and biological potential. Sea fennel flowers, stems and leaves were analyzed for essential oils (EOs) isolated by hydrodistillation, as well as non-volatiles obtained by ethanolic extraction. Limonene were found to be a dominant compound in EOs and ethanolic extracts; ranging from 57.5-74.2 % and 0.7-8.1 mg/g dry plant material, respectively. In addition total phenolic content was determined for ethanolic extracts...
July 2016: Journal of Food Science and Technology
Lynn S Adler, Lissa M Leege, Rebecca E Irwin
PREMISE OF THE STUDY: Floral evolution is frequently ascribed to selection by pollinators, but may also be shaped by antagonists. However, remarkably few studies have examined geographic mosaics in resistance to floral antagonists or the consequences for other floral interactions. METHODS: Gelsemium sempervirens experiences frequent nectar robbing in northern Georgia, but rarely in southern Georgia. We conducted common-garden experiments in both locations using genotypes from each region and measured robbing, pollinator attraction, floral attractive and defensive traits, and plant reproduction...
October 7, 2016: American Journal of Botany
Janet Ayello, Jessica Hochberg, Allyson Flower, Yaya Chu, Laxmi V Baxi, William Quish, Carmella van de Ven, Mitchell S Cairo
NK cells play a significant role in reducing relapse in patients with hematological malignancies following allogeneic stem cell transplantation but NK cell number and naturally occurring inhibitory signals limit their capability. IL-15 and 4-1BBL are important modulators of NK expansion and functional activation. With an aim to overcome these limitations, cord blood (CB) mononuclear cells (MNC) were ex-vivo expanded (EvE) for 7 days with genetically modified K562-mbIL15-41BBL (MODK562) or wildtype K562 (WTK562)...
October 17, 2016: Experimental Hematology
Jun Li, Wei Zhen, Dengkai Long, Ling Ding, Anhui Gong, Chenghong Xiao, Weike Jiang, Xiaoqing Liu, Tao Zhou, Luqi Huang
Pseudostellaria heterophylla (Miq.) Pax is a mild tonic herb widely cultivated in the Southern part of China. The tuberous roots of P. heterophylla accumulate high levels of secondary metabolism products of medicinal value such as saponins, flavonoids, and isoquinoline alkaloids. Despite numerous studies on the pharmacological importance and purification of these compounds in P. heterophylla, their biosynthesis is not well understood. In the present study, we used Illumina HiSeq 4000 sequencing platform to sequence the RNA from flowers, leaves, stem, root cortex and xylem tissues of P...
2016: PloS One
Tomasz Góral, Halina Wiśniewska, Piotr Ochodzki, Dorota Walentyn-Góral
Resistance to Fusarium head blight in 32 winter triticale and 34 winter wheat accessions was evaluated. Triticale and wheat were sown in field experiments in two locations. At the time of flowering, heads were inoculated with three Fusarium culmorum isolates. Fusarium head blight index was scored and after the harvest percentage of Fusarium damaged kernels was assessed. Grain was analysed for type B trichothecenes (deoxynivalenol and derivatives, nivalenol) and zearalenone (ZEN) content. The average Fusarium head blight indexes were 28...
October 18, 2016: Toxins
Larkin Strong, Zeno Franco, Mark Flower
No abstract text is available yet for this article.
2016: Progress in Community Health Partnerships: Research, Education, and Action
Zeno E Franco, Clinton Logan, Mark Flower, Bob Curry, Leslie Ruffalo, Ruta Brazauskas, Jeff Whittle
No abstract text is available yet for this article.
2016: Progress in Community Health Partnerships: Research, Education, and Action
Rachel A Ryskin, Zhenghan Qi, Melissa C Duff, Sarah Brown-Schmidt
Verbs often participate in more than 1 syntactic structure, but individual verbs can be biased in terms of whether they are used more often with 1 structure or the other. For instance, in a sentence such as "Bop the bunny with the flower," the phrase "with the flower" is more likely to indicate an instrument with which to "bop," rather than which "bunny" to bop. Conversely, in a sentence such as "Choose the cow with the flower," the phrase "with the flower" is more likely to indicate which "cow" to choose. An open question is where these biases come from and whether they continue to be shaped in adulthood in a way that has lasting consequences for real-time processing of language...
October 20, 2016: Journal of Experimental Psychology. Learning, Memory, and Cognition
J C Menon, J K Joseph, M P Jose, B L Dhananjaya, O V Oommen
INTRODUCTION: Snakebite is an occupational hazard causing considerable morbidity and mortality worldwide, particularly so in tropical countries like India. OBJECTIVE: The aim of this study is to (i) review the demographic, clinical and laboratory findings in patients (1051) admitted with venomous snakebite (ii) to correlate mortality, morbidity and duration of hospital stay with clinical signs, symptoms and laboratory parameters. METHODS: A retrospective study of 1051 patients treated for snakebite over 10 years (2000 - 2009) in Little Flower Hospital, Angamaly, Kerala...
August 2016: Journal of the Association of Physicians of India
Shuangshuang Zhao, Lei Zhao, Fengxia Liu, Yongzhen Wu, Zuofeng Zhu, Chuanqing Sun, Lubin Tan
Grain yield in rice (Oryza sativa L.) is closely related to leaf and flower development. Coordinative regulation of leaf, pollen, and seed development in rice as a critical biological and agricultural question should to be addressed. Here we identified two allelic rice mutants with narrow and semi-rolled leaves, named narrow and rolled leaf 2-1 (nrl2-1) and nrl2-2. Map-based molecular cloning revealed that NRL2 encodes a novel protein with unknown biochemical function. The mutation of NRL2 caused pleiotropic effects, including a reduction in the number of longitudinal veins, defective abaxial sclerenchymatous cell differentiation, abnormal tapetum degeneration and microspore development, and the formation of more slender seeds compared with the wild type (WT)...
October 20, 2016: Journal of Integrative Plant Biology
Bin Cai, Xiangxiong Kong, Chao Zhong, Suli Sun, Xiao Feng Zhou, Yin Hua Jin, Youning Wang, Xia Li, Zhendong Zhu, Jing Bo Jin
SIZ1 is a small ubiquitin-related modifier (SUMO) E3 ligase that mediates post-translational SUMO modification of target proteins and thereby regulates developmental processes and hormonal and environmental stress responses in Arabidopsis. However, the role of SUMO E3 ligases in crop plants is largely unknown. Here, we identified and characterized two Glycine max (soybean) SUMO E3 ligases, GmSIZ1a and GmSIZ1b. Expression of GmSIZ1a and GmSIZ1b was induced in response to salicylic acid (SA), heat, and dehydration treatment, but not in response to cold, ABA, and NaCl treatment...
October 20, 2016: Journal of Integrative Plant Biology
Parayil Varghese Christapher, Subramani Parasuraman, Palanimuthu Vasanth Raj, Sultan Ayesh Mohammed Saghir, Mohd Zaini Asmawi, Murugaiyah Vikneswaran
OBJECTIVE: To investigate the antihyperlipidemic, antioxidant, and cytotoxic effect of aqueous and methanol extract of leaves of Polygonum minus. MATERIALS AND METHODS: Acute antihyperlipidemic effect was studied on chemically induced hyperlipidemic rat model. Treated groups received aqueous and methanol extract of leaves of P. minus respectively (1000 mg/kg; oral) whereas standard treated group received atorvastatin (60 mg/kg; oral) for 3 consecutive days. Blood samples were collected at fixed intervals for lipid profile analysis...
July 2016: Pharmacognosy Magazine
Lekha T Pazhamala, Gaurav Agarwal, Prasad Bajaj, Vinay Kumar, Akanksha Kulshreshtha, Rachit K Saxena, Rajeev K Varshney
Seed development is an important event in plant life cycle that has interested humankind since ages, especially in crops of economic importance. Pigeonpea is an important grain legume of the semi-arid tropics, used mainly for its protein rich seeds. In order to understand the transcriptional programming during the pod and seed development, RNA-seq data was generated from embryo sac from the day of anthesis (0 DAA), seed and pod wall (5, 10, 20 and 30 DAA) of pigeonpea variety "Asha" (ICPL 87119) using Illumina HiSeq 2500...
2016: PloS One
Hai Du, Feng Ran, Hong-Li Dong, Jing Wen, Jia-Na Li, Zhe Liang
Cytochrome P450 93 family (CYP93) belonging to the cytochrome P450 superfamily plays important roles in diverse plant processes. However, no previous studies have investigated the evolution and expression of the members of this family. In this study, we performed comprehensive genome-wide analysis to identify CYP93 genes in 60 green plants. In all, 214 CYP93 proteins were identified; they were specifically found in flowering plants and could be classified into ten subfamilies-CYP93A-K, with the last two being identified first...
2016: PloS One
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