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lactate acid

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https://www.readbyqxmd.com/read/29145596/a-crispr-cas9-based-exploration-into-the-elusive-mechanism-for-lactate-export-in-saccharomyces-cerevisiae
#1
Robert Mans, Else-Jasmijn Hassing, Melanie Wijsman, Annabel Giezekamp, Jack T Pronk, Jean-Marc Daran, Antonius J A van Maris
CRISPR/Cas9-based genome editing allows rapid, simultaneous modification of multiple genetic loci in Saccharomyces cerevisiae. Here, this technique was used in a functional analysis study aimed at identifying the hitherto unknown mechanism of lactate export in this yeast. First, an S. cerevisiae strain was constructed with deletions in 25 genes encoding transport proteins, including the complete aqua(glycero)porin family and all known carboxylic-acid transporters. The 25-deletion strain was then transformed with an expression cassette for Lactobacillus casei lactate dehydrogenase (LcLDH)...
November 14, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/29144833/low-dietary-fiber-intake-increases-collinsella-abundance-in-the-gut-microbiota-of-overweight-and-obese-pregnant-women
#2
Luisa F Gomez-Arango, Helen L Barrett, Shelley A Wilkinson, Leonie K Callaway, H David McIntyre, Mark Morrison, Marloes Dekker Nitert
The gut microbiota contributes to the regulation of glucose metabolism in pregnancy. Abundance of the genus Collinsella is positively correlated with circulating insulin; however, it is unclear what determines Collinsella abundance. This study aims to validate the correlation between Collinsella and insulin and to elucidate if macronutrient intake alters Collinsella abundance and gut microbiota composition. Gut microbiota profiles were assessed by 16S rRNA sequencing in 57 overweight and 73 obese pregnant women from the SPRING (Study of PRobiotics IN Gestational diabetes) trial at 16 weeks gestation and correlated with metabolic hormone levels and macronutrient intake...
November 16, 2017: Gut Microbes
https://www.readbyqxmd.com/read/29144270/alkaline-phosphatase-in-infant-cardiopulmonary-bypass-kinetics-and-relationship-to-organ-injury-and-major-cardiovascular-events
#3
Jesse A Davidson, Tracy T Urban, Christine Baird, Suhong Tong, Alan Woodruff, Mark Twite, James Jaggers, Eric A F Simões, Paul Wischmeyer
OBJECTIVES: To determine the kinetics of alkaline phosphatase (AP) activity and concentration after infant cardiopulmonary bypass, including isoform-specific changes, and to measure the association between postoperative AP activity and major postoperative cardiovascular events, organ injury/dysfunction, and postoperative support requirements STUDY DESIGN: Prospective cohort study of 120 infants ≤120 days of age undergoing cardiopulmonary bypass. AP total and isoform-specific activity was assessed at 6 time points (preoperation, rewarming, 6, 24, 48, and 72 hours postoperation)...
November 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/29144182/plasma-lyte-148-vs-hartmann-s-solution-for-cardiopulmonary-bypass-pump-prime-a-prospective-double-blind-randomized-trial
#4
Laurence Weinberg, Elizabeth Chiam, James Hooper, Frank Liskaser, Angela Kim Hawkins, Denise Massie, Andrew Ellis, Chong O Tan, David Story, Rinaldo Bellomo
BACKGROUND: The mechanisms of acid-base changes during cardiopulmonary bypass (CPB) remain unclear. We tested the hypothesis that, when used as CPB pump prime solutions, Plasma-Lyte 148 (PL) and Hartmann's solution (HS) have differential mechanisms of action in their contribution to acid-base changes. METHODS: We performed a prospective, double-blind, randomized trial in adult patients undergoing elective cardiac surgery with CPB. Participants received a CPB prime solution of 2000 mL, with either PL or HS...
November 1, 2017: Perfusion
https://www.readbyqxmd.com/read/29142539/values-of-magnetic-resonance-imaging-and-cerebrospinal-fluid-analysis-in-the-diagnosis-of-central-nervous-system-associated-infectious-diseases
#5
Dongfeng Zhang
Objective: To discuss the roles of magnetic resonance imaging (MRI) and cerebrospinal fluid analysis in the identification of central nervous system associated infection and provide a reference for the diagnosis and treatment of central nervous system associated infectious diseases. Methods: Seventy-six patients who developed central nervous system infection and were admitted into the Henan People's Hospital between June 2014 and October 2015 were randomly selected as an observation group...
September 2017: Pakistan Journal of Medical Sciences Quarterly
https://www.readbyqxmd.com/read/29142413/antiurolithiatic-potential-of-neeri-against-calcium-oxalate-stones-by-crystallization-inhibition-free-radicals-scavenging-and-nrk-52e-cell-protection-from-oxalate-injury
#6
Parveen Kumar Goyal, Santosh Kumar Verma, Anil Kumar Sharma
Background: Neeri is a well-established polyherbal formulation prescribed for renal stones by the physicians but has not been experimentally evaluated for its antiurolithiatic potential using cell-lines. Objective: This study is aimed to scientifically substantiate the antiurolithiatic effect of Neeri extract (NRE) through calcium oxalate (CaOx) crystallization inhibition, scavenging of free radicals, and protection of renal tubular epithelial NRK-52E cells from oxalate-induced injury...
October 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/29141978/cobalamin-status-from-pregnancy-to-early-childhood-lessons-from-global-experience
#7
REVIEW
Rima Obeid, Michelle Murphy, Pol Solé-Navais, Chittaranjan Yajnik
Low cobalamin intake and status during pregnancy or lactation have been linked to adverse maternal and perinatal health outcomes, whereas low cobalamin status during early childhood is associated with impaired development in children. Women who begin pregnancy with depleted stores (low or very low plasma cobalamin) will give birth to depleted infants who are likely to develop deficiency symptoms during the first few weeks or months postpartum. Newly ingested cobalamin during pregnancy and lactation (from diet or supplements) is transferred to the child and is not likely to correct cobalamin status in depleted women...
November 2017: Advances in Nutrition
https://www.readbyqxmd.com/read/29140277/enantioselective-biosynthesis-of-l-phenyllactic-acid-by-whole-cells-of-recombinant-escherichia-coli
#8
Yibo Zhu, Ying Wang, Jiayuzi Xu, Jiahao Chen, Limei Wang, Bin Qi
BACKGROUND: l-Phenyllactic acid (l-PLA)-a valuable building block in the pharmaceutical and chemical industry-has recently emerged as an important monomer in the composition of the novel degradable biocompatible material of polyphenyllactic acid. However, both normally chemically synthesized and naturally occurring phenyllactic acid are racemic, and the product yields of reported l-PLA synthesis processes remain unsatisfactory. METHODS: We developed a novel recombinant Escherichia coli strain, co-expressing l-lactate dehydrogenase (l-LDH) from Lactobacillus plantarum subsp...
November 15, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29137367/glycolytic-reprogramming-through-pck2-regulates-tumor-initiation-of-prostate-cancer-cells
#9
Jiangsha Zhao, Jieran Li, Teresa W M Fan, Steven X Hou
Tumor-initiating cells (TICs) play important roles in tumor progression and metastasis. Identifying the factors regulating TICs may open new avenues in cancer therapy. Here, we show that TIC-enriched prostate cancer cell clones use more glucose and secrete more lactate than TIC-low clones. We determined that elevated levels of phosphoenolpyruvate carboxykinase isoform 2 (PCK2) are critical for the metabolic switch and the maintenance of TICs in prostate cancer. Information from prostate cancer patient databases revealed that higher PCK2 levels correlated with more aggressive tumors and lower survival rates...
October 13, 2017: Oncotarget
https://www.readbyqxmd.com/read/29137304/monocarboxylate-transporter-1-mct1-a-tool-to-stratify-acute-myeloid-leukemia-aml-patients-and-a-vehicle-to-kill-cancer-cells
#10
Filipa Lopes-Coelho, Carolina Nunes, Sofia Gouveia-Fernandes, Rita Rosas, Fernanda Silva, Paula Gameiro, Tânia Carvalho, Maria Gomes da Silva, José Cabeçadas, Sérgio Dias, Luís G Gonçalves, Jacinta Serpa
Dysregulation of glucose/lactate dynamics plays a role in cancer progression, and MCTs are key elements in metabolic remodeling. VEGF is a relevant growth factor in the maintenance of bone marrow microenvironment and it is also important in hematological diseases. Our aim was to investigate the role of VEGF in the metabolic adaptation of Acute myeloid leukemia (AML) cells by evaluating the metabolic profiles and cell features according to the AML lineage and testing lactate as a metabolic coin. Our in vitro results showed that AML promyelocytic (HL60) and monocytic (THP1) (but not erythroid- HEL) lineages are well adapted to VEGF and lactate rich environment...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29136753/-metabolomics-study-of-tris-2-chloroethyl-phosphate-induced-hepaotoxicity-and-nephrotoxicity-in-sprague-dawley-rats
#11
W Q Yang, F Zhao, L Li, Y J Fang
Objective: To discuss the potential toxic target organ and the toxic effects and mechanisms of tris (2-chloroethyl) phosphate (TCEP) on SD rats. Methods: 40 female SD rats weaning from milk for 21 days, weighted (50±2.3)g were selected as subjects and marked by the weight. They were randomly divided into 4 groups, namely control group, 50 (L), 100 (M) and 250 (H) mg·kg(-1)·d(-1) dose of TCEP group. Each group has 10 rats, and administrated the corresponding dose of drug or vehicle by mouth, quaque die for 60 days...
November 6, 2017: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29136560/cytoprotective-effects-of-glycyrrhetinic-acid-liposome-against-cyclophosphamide-induced-cystitis-through-inhibiting-inflammatory-stress
#12
Bo Ge, Daping Yang, Xinmou Wu, Jing Zhu, Wu Wei, Bin Yang
This study was designed to investigate the pharmacological efficacy of glycyrrhetinic acid liposome (GAL) against female mice with nonbacterial cystitis induced by cyclophosphamide (CPS). Mice in different groups were subjected to tests for lactate dehydrogenase (LD), cytokine contents (IL-6, TNF-α) in serum, and histological changes in bladder tissue and to immunoassays. As a result, cyclophosphamide-induced cystitis in mice showed an increased LD level in serum, and the contents of cytokines (IL-6, TNF-α) were elevated...
November 11, 2017: International Immunopharmacology
https://www.readbyqxmd.com/read/29135861/in-vitro-and-in-vivo-antimelanoma-effect-of-ethyl-ester-cyclohexyl-analog-of-ethylenediamine-dipropanoic-acid
#13
Andjelka M Isakovic, Sasa M Petricevic, Slavica M Ristic, Dusan M Popadic, Tamara K Kravic-Stevovic, Nevena S Zogovic, Jelena M Poljarevic, Tatjana V Zivanovic Radnic, Tibor J Sabo, Aleksandra J Isakovic, Ivanka D Markovic, Vladimir S Trajkovic, Sonja T Misirlic-Dencic
Melanoma, an aggressive skin tumor with high metastatic potential, is associated with high mortality and increasing morbidity. Multiple available chemotherapeutic and immunotherapeutic modalities failed to improve survival in advanced disease, and the search for new agents is ongoing. The aim of this study was to investigate antimelanoma effects of O,O-diethyl-(S,S)-ethylenediamine-N,N'di-2-(3-cyclohexyl) propanoate dihydrochloride (EE), a previously synthesized and characterized organic compound. Mouse melanoma B16 cell viability was assessed using acid phosphatase, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, sulforhodamine B, and lactate dehydrogenase assays...
November 13, 2017: Melanoma Research
https://www.readbyqxmd.com/read/29134060/modelling-pyruvate-dehydrogenase-under-hypoxia-and-its-role-in-cancer-metabolism
#14
Filmon Eyassu, Claudio Angione
Metabolism is the only biological system that can be fully modelled at genome scale. As a result, metabolic models have been increasingly used to study the molecular mechanisms of various diseases. Hypoxia, a low-oxygen tension, is a well-known characteristic of many cancer cells. Pyruvate dehydrogenase (PDH) controls the flux of metabolites between glycolysis and the tricarboxylic acid cycle and is a key enzyme in metabolic reprogramming in cancer metabolism. Here, we develop and manually curate a constraint-based metabolic model to investigate the mechanism of pyruvate dehydrogenase under hypoxia...
October 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/29132539/the-diabetic-heart-utilizes-ketone-bodies-as-an-energy-source
#15
Yuji Mizuno, Eisaku Harada, Hitoshi Nakagawa, Yoshinobu Morikawa, Makoto Shono, Fumihito Kugimiya, Michihiro Yoshimura, Hirofumi Yasue
BACKGROUND: Diabetic heart is characterized by failure of insulin to increase glucose uptake and increasingly relies on free fatty acids (FFAs) as a source of fuel in animal models. However, it is not well known how cardiac energy metabolism is altered in diabetic hearts in humans. We examined cardiac fuel metabolism in the diabetics as compared to non-diabetics who underwent cardiac catheterization for heart diseases. MATERIAL AND METHODS: The study subjects comprised 81 patients (male 55, female 26, average age 63...
December 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/29131416/characterization-of-sweat-induced-with-pilocarpine-physical-exercise-and-collected-passively-by-metabolomic-analysis
#16
S L Souza, G Graça, A Oliva
BACKGROUND/PURPOSE: The elimination of the pain associated with needle picking is a strong motivation for the development of clinical non-invasive diagnostic methods. Sweat has been described as an alternative biological sample that may have a direct relation to the plasma composition. MATERIALS AND METHODS: In this study, analysis of sweat of human volunteers obtained by induction with pilocarpine is compared with sweat samples obtained by physical exercise and by passive collection along 7 hours...
November 12, 2017: Skin Research and Technology
https://www.readbyqxmd.com/read/29131337/effect-of-farming-system-on-donkey-milk-composition
#17
Emanuela Valle, Luisa Pozzo, Marzia Giribaldi, Domenico Bergero, Maria Silvia Gennero, Daniela Dezzutto, Amy McLean, Giorgio Borreani, Mauro Coppa, Laura Cavallarin
BACKGROUND: Donkey milk is considered a functional food for sensitive consumers, such as children allergic to cow milk. No information is available regarding the effect of farming systems on the quality of donkey milk. The study was aimed at evaluating the effect of the farming system and lactation stage on gross composition, fat-soluble vitamins and fatty acids (FA) in donkey milk. RESULTS: Individual milk samples were collected from lactating jennies (n=53) on the six of the largest farms located in North West Italy...
November 13, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29130749/does-maternal-synbiotic-supplementation-affect-conjugated-linoleic-acid-level-in-breast-milk-a-randomized-placebo-controlled-clinical-trial
#18
Leila Nikniaz, Reza Mahdavi, Alireza Ostadrahimi, Zeinab Nikniaz, Soghra Aliasgharzadeh
AIM: As conjugated linoleic acid (CLA)is a potential growth promotor in newborns, the present pilot study aimed at measuring the effect of synbiotic supplementation on breast milk CLA level. MATERIALS AND METHODS: Sixty lactating mothers were randomly allocated to take either synbiotic (n = 31) or placebo (n = 29) for 30 days. Maternal anthropometric indices were measured at the onset and end of the study by standard methods and body mass index was calculated...
November 13, 2017: Breastfeeding Medicine: the Official Journal of the Academy of Breastfeeding Medicine
https://www.readbyqxmd.com/read/29130237/in-situ-forming-oxidised-hyaluronic-acid-adipic-acid-dihydrazide-hydrogel-for-prevention-of-epidural-fibrosis-after-laminectomy
#19
M H Hu, K C Yang, Y H Sun, Y C Chen, S H Yang, F H Lin
Post-operative epidural fibrosis is a biological response after laminectomy that may lead to clinical symptoms, such as radicular pain. An ideal material for prevention of epidural fibrosis should be able to inhibit fibroblast adhesions and reduce formation of scar tissue. An injectable hydrogel would be the material of choice for this purpose, since it could fill an irregular surgical defect completely, gelate in situ and be delivered in a minimally-invasive manner. The objective of this study was to evaluate, in vitro and in vivo, the cytocompatibility and anti-adhesive effect of an oxidised hyaluronic acid/adipic acid dihydrazide (oxi-HA/ADH) hydrogel...
November 13, 2017: European Cells & Materials
https://www.readbyqxmd.com/read/29130117/correction-to-the-metabolic-cost-of-nesting-body-condition-and-blood-parameters-of-caiman-crocodilus-and-melanosuchus-niger-in-central-amazonia
#20
José António Lemos Barão-Nóbrega, Boris Marioni, Robinson Botero-Arias, António José Arsénia Nogueira, Emerson Silva Lima, William Ernest Magnusson, Ronis Da Silveira, Jaydione Luiz Marcon
Although nesting ecology is well studied in several crocodilian species, it is not known how nest attendance influences physiology and body condition of nesting females. In this study, we describe body condition and serum biochemical values of nesting female, non-nesting female and male spectacled caiman (Caiman crocodilus) and black caiman (Melanosuchus niger) in two areas of Central Amazonia. We also evaluated the effect of nest age and nest distance to water on body condition and blood parameters of nesting females...
November 13, 2017: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
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