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https://www.readbyqxmd.com/read/29331418/non-coding-rnas-in-cancer-stem-cells
#1
Huiwen Yan, Pengcheng Bu
Cancer stem cells (CSCs) have been shown to play a key role in tumor initiation, progression, metastasis, and therapy resistance. Despite their potential clinical importance, the mechanism of CSC regulation is not well understood. Recent evidence suggests that different types of non-coding RNAs (ncRNA), such as microRNA (miRNA) and long non-coding RNA (LncRNA), play a role in regulating CSC growth and replication by modulating transcription factors and downstream signaling pathways activated in CSCs. Here, we review the recent major findings about how they affect stem cell quality acquisition and maintenance in CSCs, as well as metastasis and therapy resistance...
January 10, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29328463/construction-and-investigation-of-lncrna-associated-cerna-regulatory-network-in-papillary-thyroid-cancer
#2
Yangjing Zhao, Hui Wang, Chengjiang Wu, Meina Yan, Haojie Wu, Jingzhe Wang, Xinxin Yang, Qixiang Shao
Increasing evidence has experimentally proved the competitive endogenous RNA (ceRNA) hypothesis that long non-coding RNA (lncRNA) can affect the expression of RNA targets by competitively combining microRNA (miRNA) via miRNA response elements. However, an extensive ceRNA network of thyroid carcinoma in a large cohort has not been evaluated. We analyzed the RNAseq and miRNAseq data of 348 cases of primary papillary thyroid cancer (PTC) patients with clinical information downloaded from The Cancer Genome Atlas (TCGA) project to search for potential biomarkers or therapeutic targets...
January 10, 2018: Oncology Reports
https://www.readbyqxmd.com/read/29323740/long-non-coding-rna-asbel-promotes-osteosarcoma-cell-proliferation-migration-and-invasion-by-regulating-microrna-21
#3
Jianhui Zhao, Zhongli Gao, Chao Zhang, Han Wu, Rui Gu, Rui Jiang
Osteosarcoma is the most common malignant bone tumor in children and adolescents with high rate of incidence, high frequency of recurrence and high degree of metastasis. This study aimed to investigate the effects of long noncoding RNA antisense ncRNA in the Abundant in neuroepithelium area (ANA)/B-cell translocation gene 3 (BTG3) locus (lncRNA ASBEL) on the pathogenesis of osteosarcoma. The expression levels of ASBEL in human osteoblast cells and human osteosarcoma cells were evaluated using qRT-PCR. Effects of ASBEL knockdown on cell viability, migration and invasion were detected using trypan blue exclusion assay, cell migration and invasion assay, respectively...
January 11, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29323698/xist-accelerates-neuropathic-pain-progression-through-regulation-of-mir-150-and-zeb1-in-cci-rat-models
#4
Xue-Tao Yan, Jing-Min Lu, Yu Wang, Xiao-Li Cheng, Xiang-Hu He, Wen-Zhong Zheng, Hu Chen, Yan-Lin Wang
LncRNAs are reported to participate in neuropathic pain development. LncRNA X-inactive specific transcript (XIST) is involved in the progression of various cancers. However, the role of XIST in neuropathic pain remains unclear. In our present study, we established a chronic constriction injury (CCI) rat model and XIST was found to be greatly upregulated both in the spinal cord tissues and in the isolated microglias of CCI rats. Inhibition of XIST inhibited neuropathic pain behaviors including mechanical and thermal hyperalgesia...
January 11, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29322448/characterization-of-circular-rnas-circrna-associated-with-the-translation-machinery
#5
Deniz Bartsch, Anne Zirkel, Leo Kurian
A substantial proportion of the currently annotated genes in eukaryotes are proposed to function as RNA molecules (>200 bp) with no significant protein coding potential, currently classified as long noncoding RNAs (lncRNA). A distinct subgroup of lncRNAs is circular RNAs (circRNAs), which can be easily identified by unique junction reads, resulting from their biogenesis. CircRNAs are largely cytosolic and thought to either code for micro-peptides or facilitate gene regulation by sequestering microRNAs (miRNAs) or RNA-binding proteins (RBPs) from their targets...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29321088/upregulation-of-long-non-coding-rna-tug1-promotes-bladder-cancer-cell-5-proliferation-migration-and-invasion-by-inhibiting-mir-29c
#6
Peng Guo, Guohui Zhang, Jialin Meng, Qian He, Zhihui Li, Yawei Guan
Bladder cancer (BC) is one of the leading causes of cancer-related death in the word. Long non-coding RNA (lncRNA) taurine-upregulated gene 1 (TUG1) plays an important role in the development and progression of numerous cancers, including BC. However, the exact role of TUG1 in modulating BC progression is still poorly known. In this study, we found that TUG1 was upregulated and microRNA-29c (miR-29c) was downregulated in BC tissues and cell lines. Overexpression of TUG1 promoted the cell proliferation of T24 and EJ cells, whereas TUG1 knockdown had the opposite effect...
January 10, 2018: Oncology Research
https://www.readbyqxmd.com/read/29321082/long-noncoding-rna-atb-promotes-proliferation-migration-and-invasion-in-bladder-cancer-by-suppressing-microrna-126
#7
Xingquan Zhai, Wei Xu
This study was aimed to explore the biological functions of long non-coding RNA activated by transforming growth factor-beta (lncRNA-ATB) in bladder cancer cells. For the expressions of lncRNA-ATB, miR-126 and KRAS, T24 cells were transfected with their specific vectors/shRNA or mimic/inhibitor. Then, cell viability, migration, invasion and apoptosis, as well as the protein levels of apoptosis-related factors and PI3K/AKT and mTOR signal pathways were measured, respectively. Further, the relationships of lncRNA-ATB and miR-126 or miR-126 and KRAS were analyzed by dual-luciferase report assay...
January 10, 2018: Oncology Research
https://www.readbyqxmd.com/read/29295724/long-non-coding-rna-cat104-promotes-cell-viability-migration-and-invasion-in-gastric-carcinoma-cells-through-activation-of-microrna-381-inhibiting-zinc-finger-e-box-binding-homeobox-1-zeb1-expression
#8
Gang Yuan, Jingzi Quan, Dongfang Dong, Qunying Wang
Gastric carcinoma (GC) remains the second leading cause of cancer-related death worldwide. Good biomarker is of paramount importance for GC therapy. This study was aimed to assess the role of long non-coding RNA (LncRNA) CAT104 in GC. We found that CAT104 was highly expressed in human GC NCI-N87, SGC7901, BGC823, BGC803 and AGS cells. Suppression of CAT104 decreased NCI-N87 cell viability, migration and invasion, but promoted apoptosis. CAT104 knockdown enhanced the expression of microRNA-381 (miR-381) expression in NCI-N87 cells...
January 2, 2018: Oncology Research
https://www.readbyqxmd.com/read/29286144/effect-of-long-non-coding-rna-pvt1-on-cell-proliferation-and-migration-in-melanoma
#9
Lamei Chen, Dongmei Ma, Yuanyuan Li, Xiaoying Li, Lei Zhao, Jing Zhang, Yali Song
The present study aimed to investigate the potential role of the long non‑coding RNA (lncRNA) Pvt1 oncogene (non‑protein coding) (PVT1) in the progression and metastasis of malignant melanoma, and to reveal its possible molecular mechanisms. The expression of lncRNA PVT1 in melanoma tissues and adjacent normal skin from patients with melanoma, and in the melanoma A‑375 and sk‑mel‑5 cell lines, was analyzed using reverse transcription‑quantitative polymerase chain reaction and western blot analyses...
December 20, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29284304/altered-long-non-coding-rna-transcriptomic-profiles-in-ischemic-stroke
#10
Wenzhen He, Duncan Wei, De Cai, Siqia Chen, Shunxian Li, Wenjie Chen
BACKGROUND: Our previous study described the important regulatory roles of microRNAs (miRNAs) in ischemic stroke. However, the functional significance of long non coding RNA (lncRNAs) in ischemic stroke was largely unknown. This study was aimed to identify the lncRNA profiling and elucidate the regulatory mechanisms in pathophysiology of stroke. MATERIALS AND METHODS: Herein, we used RNA sequencing to sequence the RNA from blood of three ischemic stroke patients and three normal controls...
December 28, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/29277611/long-non-coding-rna-pvt1-5-promotes-cell-proliferation-by-regulating-mir-126-slc7a5-axis-in-lung-cancer
#11
Hongliang Li, Shuxian Chen, Jia Liu, Xiaopeng Guo, Xudong Xiang, Tianqi Dong, Pengzhan Ran, Qian Li, Bei Zhu, Xiyu Zhang, Dan Wang, Chunjie Xiao, Shangyong Zheng
Dysregulated long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) play key roles in the development of human cancers. The lncRNA plasmacytoma variant translocation 1 (PVT1) is reported to be an oncogene in a variety of cancers. However, the roles of PVT1-5 and its related miRNAs in lung cancer are poorly understood. In this study, we found that PVT1-5 expression was significantly increased in lung cancer tissues and cell lines. By using biotin-labeled lncRNA-PVT1-5 probe for miRNA in vivo precipitation (miRIP) in lung cancer cells and dual-luciferase reporterassays, we identified that miR-126 was associated with lncRNA-PVT1-5...
December 23, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29259349/are-fusion-transcripts-in-relapsed-metastatic-head-and-neck-cancer-patients-predictive-of-response-to-anti-egfr-therapies
#12
Paolo Bossi, Marco Siano, Cristiana Bergamini, Maria Cossu Rocca, Andrea P Sponghini, Marco Giannoccaro, Luca Tonella, Alessandro Paoli, Edoardo Marchesi, Federica Perrone, Silvana Pilotti, Laura D Locati, Silvana Canevari, Lisa Licitra, Loris De Cecco
Prediction of benefit from combined chemotherapy and the antiepidermal growth factor receptor cetuximab is a not yet solved question in head and neck squamous cell carcinoma (HNSCC). In a selected series of 14 long progression-free survival (PFS) and 26 short PFS patients by whole gene and microRNA expression analysis, we developed a model potentially predictive of cetuximab sensitivity. To better decipher the "omics" profile of our patients, we detected transcript fusions by RNA-seq through a Pan-Cancer panel targeting 1385 cancer genes...
2017: Disease Markers
https://www.readbyqxmd.com/read/29246322/long-noncoding-rna-hcal-facilitates-the-growth-and-metastasis-of-hepatocellular-carcinoma-by-acting-as-a-cerna-of-laptm4b
#13
Cheng-Rong Xie, Fei Wang, Sheng Zhang, Fu-Qiang Wang, Sen Zheng, Zhao Li, Jie Lv, He-Qiang Qi, Qin-Liang Fang, Xiao-Min Wang, Zhen-Yu Yin
Long noncoding RNAs (lncRNAs) are a new class of regulatory noncoding RNAs. Emerging evidences indicate that lncRNAs play a critical role in the development of hepatocellular carcinoma (HCC). Although several lncRNAs have been annotated, the association of most lncRNAs with HCC is unknown. In this study, we investigated lncRNA alterations in HCC by performing lncRNA microarray analysis. We identified a novel lncRNA called HCC-associated lncRNA (HCAL) that was highly expressed in HCC tissues. HCAL upregulation was clinically associated with poor differentiation, intravascular cancer embolus, and decreased survival of patients with HCC...
December 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/29246025/mirna-and-lncrna-as-biomarkers-in-cholangiocarcinoma-cca
#14
REVIEW
Bo Zheng, Seogsong Jeong, Yanjing Zhu, Lei Chen, Qiang Xia
The microRNAs are a group of 20 nucleotides-long non-coding RNAs. By binding to the 3'UTR region of target mRNA, microRNAs can perform extensive actions mediating gene expression at post-trancriptional stages. It makes microRNAs serve as very crucial regulators in various biological progress including carcinogenesis. Long non-coding RNAs, however, are a subgroup of RNA with the length of 200 nucleotides. Unlike microRNAs, long non-coding RNAs can form secondary of tertiary domain based on their length. With the ability of directly interacting with DNA, RNA, protein, long non-coding RNAs have promoting or inhibitive functions in gene expression regulation...
November 21, 2017: Oncotarget
https://www.readbyqxmd.com/read/29238035/long-non-coding-rna-meg3-functions-as-a-competing-endogenous-rna-to-regulate-ischemic-neuronal-death-by-targeting-mir-21-pdcd4-signaling-pathway
#15
Honglin Yan, Jie Rao, Jingping Yuan, Likun Gao, Wenxian Huang, Lina Zhao, Jiacai Ren
Long non-coding RNA (lncRNA) maternally expressed gene 3 (MEG3) has been demonstrated as an important regulator in diverse human cancers. However, its function and regulatory mechanism in ischemic stroke remains largely unknown. Here, we report that MEG3 is physically associated with microRNA-21 (miR-21), while miR-21 is downregulated following ischemia in the ischemic core in vitro and in vivo, which is opposite to MEG3. Besides, overexpression of miR-21 protects oxygen-glucose deprivation and reoxygenation (OGD/R)-induced apoptotic cell death...
December 13, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/29236319/effects-of-long-non-coding-rna-host2-on-cell-migration-and-invasion-by-regulating-microrna-let-7b-in-breast-cancer
#16
Peng-Wei Lu, Lin Li, Fang Wang, Yuan-Ting Gu
The study intends to investigate the effects of long non-coding RNA HOST2 (lncRNA HOST2) on cell migration and invasion by regulating microRNA let-7b (let-7b) in breast cancer. Breast cancer and adjacent normal tissues were collected from 98 patients with breast cancer. Breast cancer MCF-7 cells were divided into the blank, negative control (NC), pcDNA3-Mock, siHOST2, let-7b inhibitor, pcDNA3-HOST2, let-7b mimic, pcDNA3-HOST2 + let-7b mimic and siHOST2 + let-7b inhibitor groups. RT-qPCR was used to detect the mRNA expressions of HOST2, let-7b and c-Myc...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29231270/profiling-and-characterization-of-micrornas-responding-to-sodium-butyrate-treatment-in-a549-cells
#17
Xiaoqiang Xiao, Yingjie Cao, Haoyu Chen
Butyrate inhibits growth of lung cancer. However, the molecular mechanism is still unclear. Here we profiled miRNAs that responded to sodium butyrate(NaB) stimulation in A549 cells, a non-small cell lung cancer cell line, using microarray. We found 33 up-regulated microRNAs and 22 down-regulated microRNAs (log2≥1.5 folds, P-value<0.05). The expression of miR-3935, miR-574-3p and miR-494-3p was confirmed by realtime qPCR. Then,we explored their potential targets of miR-574-3p and miR-494-3p using long noncoding RNA(LncRNA) microarray...
December 12, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29226325/lncrna-malat1-induces-colon-cancer-development-by-regulating-mir-129-5p-hmgb1-axis
#18
Ying Jie, Haijian Zhao
Recent studies have exhibited significant roles of lncRNAs in various tumors' development, including colon cancer. Our study focused on the biological roles of lncRNA MALAT1 in colon cancer. In our study, it was demonstrated that MALAT1 was upregulated in human colon cancer cell lines including Lovo, HCT116, SW480 and HT29 cells compared to the normal human intestinal epithelial HIEC cells. Moreover, we observed that miR-129-5p was downregulated in colon cancer cells with a significant increase of HMGB1 expression...
December 11, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29224950/mrna-microrna-and-lncrna-as-novel-bladder-tumor-markers
#19
REVIEW
Edyta Wieczorek, Edyta Reszka
Early detection of bladder cancer (BC) is essential for improvement of the patient's prognosis and general survival rates. Current diagnostic methods are still limited, so new specific and cost-effective biomarkers are emerging as the noninvasive tools in treatment decisions in recurrent BC. Gene expression and epigenetic profile can be analysed using quantitative real-time-PCR (qRT-PCR) method in urine, blood and tissue. This review provides an update of recent findings on BC molecular profile as novel markers in diagnosis and prognosis of bladder tumors...
December 7, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29221210/the-molecular-classification-of-astrocytic-tumors
#20
Chen-Xue Mao, Ji-Ye Yin, Ying Zhang, Zhi-Bin Wang, Zhi-Quan Yang, Zheng-Wen He, Xiang-Min Li, Xiao-Yuan Mao, Ru-Tao Cui, Xue-Jun Li, Xi Li, Wei Zhang, Hong-Hao Zhou, Zhao-Qian Liu
Aim: This study will explore the genetic and epigenetic alterations in astrocytomas, and identify the critical molecular signatures and signaling pathways for prognosis assessment by multiplatform comprehensive analysis. Method: We performed integration analyses of incorporating DNA methylation, mRNA expression, microRNA expression, and long non-coding RNA (lncRNA) expression in 33 astrocytic tumor tissues and 9 non-tumor brain tissues. Result: We observed that 11,795 DNA methylation sites, 3,627 genes, 136 microRNAs, and 3,334 lncRNAs were significantly differential between tumors and non-tumor brain tissues, and the filtered signatures through comprehensive analysis were significantly enriched in calcium signaling pathway...
November 10, 2017: Oncotarget
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