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Oligomerization

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https://www.readbyqxmd.com/read/29453800/atomic-structures-of-corkscrew-forming-segments-of-sod1-reveal-varied-oligomer-conformations
#1
Smriti Sangwan, Michael R Sawaya, Kevin A Murray, MichaelP Hughes, David S Eisenberg
The aggregation cascade of disease-related amyloidogenic proteins, terminating in insoluble amyloid fibrils, involves intermediate oligomeric states. The structural and biochemical details of these oligomers have been largely unknown. Here we report crystal structures of variants of the cytotoxic oligomer-forming segment residues 28-38 of the ALS-linked protein, SOD1. The crystal structures reveal three different architectures: corkscrew oligomeric structure, non-twisting curved sheet structure and a steric zipper proto-filament structure...
February 17, 2018: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29451229/m-aaa-proteases-mitochondrial-calcium-homeostasis-and-neurodegeneration
#2
REVIEW
Maria Patron, Hans-Georg Sprenger, Thomas Langer
The function of mitochondria depends on ubiquitously expressed and evolutionary conserved m-AAA proteases in the inner membrane. These ATP-dependent peptidases form hexameric complexes built up of homologous subunits. AFG3L2 subunits assemble either into homo-oligomeric isoenzymes or with SPG7 (paraplegin) subunits into hetero-oligomeric proteolytic complexes. Mutations in AFG3L2 are associated with dominant spinocerebellar ataxia (SCA28) characterized by the loss of Purkinje cells, whereas mutations in SPG7 cause a recessive form of hereditary spastic paraplegia (HSP7) with motor neurons of the cortico-spinal tract being predominantly affected...
February 16, 2018: Cell Research
https://www.readbyqxmd.com/read/29450537/enhanced-expression-of-nlrp3-inflammasome-related-inflammation-in-diabetic-retinopathy
#3
Hui Chen, Xiongze Zhang, Nanying Liao, Lan Mi, Yuting Peng, Bing Liu, Shaochong Zhang, Feng Wen
Purpose: The aim of this study was to determine the association between nucleotide-binding and oligomerization domain (NOD)-like receptors (NLRs) family, pyrin domain-containing 3 (NLRP3) inflammasome-induced inflammation and disease severity in diabetic retinopathy (DR). Methods: Blood samples were collected from 64 patients with diabetes (DR, 43; without DR, 21) and 25 healthy controls. The protein and mRNA expression levels of NLRP3 inflammasomes in peripheral blood mononuclear cells were determined using western blotting and quantitative real-time reverse transcription-PCR...
February 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29450114/flavonoid-rich-extract-of-chromolaena-odorata-modulate-circulating-glp-1-in-wistar-rats-computational-evaluation-of-tgr5-involvement
#4
Olaposi Idowu Omotuyi, Oyekanmi Nash, Olumide Kayode Inyang, Joyce Ogidigo, Ojochenemi Enejoh, Okiemute Okpalefe, Tsuyoshi Hamada
Chromolaena odorata is a major bio-resource in folkloric treatment of diabetes. In the present study, its anti-diabetic component and underlying mechanism were investigated. A library containing 140 phytocompounds previously characterized from C. odorata was generated and docked (Autodock Vina) into homology models of dipeptidyl peptidase (DPP)-4, Takeda-G-protein-receptor-5 (TGR5), glucagon-like peptide 1 (GLP1) receptor, renal sodium dependent glucose transporter (SGLUT)-1/2 and nucleotide-binding oligomerization domain (NOD) proteins 1&2...
February 2018: 3 Biotech
https://www.readbyqxmd.com/read/29450109/determination-of-diffusion-coefficients-in-live-cells-using-fluorescence-recovery-after-photobleaching-with-wide-field-fluorescence-microscopy
#5
Akira Kitamura, Masataka Kinjo
Fluorescence recovery after photobleaching (FRAP) enables characterization of quantitative dynamic properties such as diffusion coefficients of fluorescent molecules in living cells by analyzing the recovery of fluorescence intensity after photobleaching in a specific cellular compartment or area. To quantitatively determine high intracellular diffusion coefficients, a suitable optical system as well as an appropriate model for fast diffusion analysis is necessary. Here, we propose a procedure to quantify the diffusion coefficient of rapidly-diffusing fluorescent molecules that makes use of an epi-fluorescence microscope with a photobleaching laser in combination with established models for diffusion analysis...
2018: Biophysics and Physicobiology
https://www.readbyqxmd.com/read/29448705/dimerization-of-ethylene-to-butenes-over-ordered-mesoporous-silica-sba-15-supported-ni-al-catalysts-with-different-morphologies
#6
Tae-Wan Kim, Jong Won Jun, Seok Il Hong, Chul-Ung Kim
A series of ordered mesoporous silica (OMS) SBA-15 supports with different morphologies were prepared by different synthetic methods to investigate the effect of the characteristics of the morphology of OMS on the ethylene dimerization outcomes. After additions of Ni and Al species into the SBA-15 support, a dimerization reaction of ethylene was performed using a fixed-bed reactor. Rod-type Ni-Al-SBA-15 with a small micron size showed better catalytic performance compared to those of the other catalysts. From these catalytic results, the particle size and morphology of a SBA-15 support critically influenced the catalytic activities and lifetimes of the dimerization catalysts...
March 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29448037/quantifying-membrane-protein-oligomerization-with-fluorescence-cross-correlation-spectroscopy
#7
Megan J Kaliszewski, Xiaojun Shi, Yixuan Hou, Ryan Lingerak, Soyeon Kim, Paul Mallory, Adam W Smith
Fluorescence cross-correlation spectroscopy (FCCS) is an advanced fluorescence technique that can quantify protein-protein interactions in vivo. Due to the dynamic, heterogeneous nature of the membrane, special considerations must be made to interpret FCCS data accurately. In this study, we describe a method to quantify the oligomerization of membrane proteins tagged with two commonly used fluorescent probes, mCherry (mCH) and enhanced green (eGFP) fluorescent proteins. A mathematical model is described that relates the relative cross-correlation value (f c ) to the degree of oligomerization...
February 12, 2018: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/29447978/the-influence-of-procyanidins-isolated-from-small-leaved-lime-flowers-tilia-cordata-mill-on-human-neutrophils
#8
Monika E Czerwińska, Marta K Dudek, Karolina A Pawłowska, Anna Pruś, Maria Ziaja, Sebastian Granica
Linden flower is a wildly used plant material among patients in the treatment of common cold symptoms and mucosa inflammations. However, the structure and bioactivity of flavan-3-ol derivatives present in infusions from flowers of Tilia cordata have not been studied so far. The aim of current study was to isolate and identify main procyanidins present in the flowers of small-leaved lime and to evaluate their influence on the inflammatory response of human neutrophils ex vivo. The chemical structure of isolated compounds was established by 1D and 2D NMR experiments...
February 12, 2018: Fitoterapia
https://www.readbyqxmd.com/read/29447697/herpes-simplex-virus-1-vp22-inhibits-aim2-dependent-inflammasome-activation-to-enable-efficient-viral-replication
#9
Yuhei Maruzuru, Takeshi Ichinohe, Ryota Sato, Kensuke Miyake, Tokuju Okano, Toshihiko Suzuki, Takumi Koshiba, Naoto Koyanagi, Shumpei Tsuda, Mizuki Watanabe, Jun Arii, Akihisa Kato, Yasushi Kawaguchi
The AIM2 inflammasome is activated by DNA, leading to caspase-1 activation and release of pro-inflammatory cytokines interleukin 1β (IL-1β) and IL-18, which are critical mediators in host innate immune responses against various pathogens. Some viruses employ strategies to counteract inflammasome-mediated induction of pro-inflammatory cytokines, but their in vivo relevance is less well understood. Here we show that the herpes simplex virus 1 (HSV-1) tegument protein VP22 inhibits AIM2-dependent inflammasome activation...
February 14, 2018: Cell Host & Microbe
https://www.readbyqxmd.com/read/29447116/amyloid-toxicity-in-alzheimer-s-disease
#10
REVIEW
Allison B Reiss, Hirra A Arain, Mark M Stecker, Nicolle M Siegart, Lora J Kasselman
A major feature of Alzheimer's disease (AD) pathology is the plaque composed of aggregated amyloid-β (Aβ) peptide. Although these plaques may have harmful properties, there is much evidence to implicate soluble oligomeric Aβ as the primary noxious form. Aβ oligomers can be generated both extracellularly and intracellularly. Aβ is toxic to neurons in a myriad of ways. It can cause pore formation resulting in the leakage of ions, disruption of cellular calcium balance, and loss of membrane potential. It can promote apoptosis, cause synaptic loss, and disrupt the cytoskeleton...
February 15, 2018: Reviews in the Neurosciences
https://www.readbyqxmd.com/read/29446720/electrochemistry-of-alzheimer-disease-amyloid-beta-peptides
#11
Ana-Maria Chiorcea-Paquim, Teodor Adrian Enache, Ana Maria Oliveira-Brett
Alzheimer's disease (AD) is a widespread form of dementia that is estimated to affect 44.4 million people worldwide. AD pathology is closely related to the accumulation of amyloid beta (Aβ) peptides in fibrils and plagues, the small oligomeric intermediate species formed during the Aβ peptides aggregation presenting the highest neurotoxicity. This review discusses the recent advances on the Aβ peptides electrochemical characterisation. The Aβ peptides oxidation at a glassy carbon electrode occurs in one or two steps, depending on the amino acid sequence, length and content...
February 13, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29445937/secondary-hemophagocytic-syndrome-associated-with-cog6-gene-defect-report-and-review
#12
Nouf Althonaian, Abdulrahman Alsultan, Eva Morava, Majid Alfadhel
Hemophagocytic lymphohistiocytosis (HLH) is a rare but potentially fatal disease that is characterized by proliferation and infiltration of hyperactivated macrophages and T-lymphocytes. Clinically, it is characterized by prolonged fever, hepatosplenomegaly, hypertriglyceridemia, hypofibrinogenemia, pancytopenia, and hemophagocytosis in the bone marrow, spleen, or lymph nodes. It can be classified as primary if it is due to a genetic defect, or secondary if it is due to a different etiology such as severe infection, immune deficiency syndrome, rheumatological disorder, malignancy, and inborn errors of metabolism such as galactosemia, multiple sulfatase deficiency, lysinuric protein intolerance, Gaucher disease, Niemann-Pick disease, Wolman disease, propionic acidemia, methylmalonic acidemia, biotinidase deficiency, cobalamin C defect, galactosialidosis, Pearson syndrome, and long-chain 3-hydroxyacyl-CoA dehydrogenase (LCHAD) deficiency...
February 15, 2018: JIMD Reports
https://www.readbyqxmd.com/read/29445379/contribution-of-regulatory-t-cells-in-nucleotide-binding-oligomerization-domain-2-response-to-influenza-virus-infection
#13
Benoit Egarnes, Jean Gosselin
Influenza A virus (IAV) is recognized to cause severe pulmonary illnesses in humans, particularly in elderly and children. One of the features associated with IAV infection is an excessive lung inflammation due to an uncontrolled immune response. The nucleotide-binding oligomerization domain 2 (NOD2) receptor is known to recognize ssRNA viruses such as IAV, but its role in the inflammatory process during viral infections remains to be clarified. In a previous report, we have shown that activation of NOD2 with muramyl dipeptide (MDP) significantly reduces both viral loads and lung inflammation and also improves pulmonary function during IAV infection...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29444458/effect-of-glycated-insulin-on-the-blood-brain-barrier-permeability-an-in-vitro-study
#14
Leila Shahriyary, Gholamhossein Riazi, Mohammad Reza Lornejad, Mansoureh Ghezlou, Bahareh Bigdeli, Behdad Delavari, Fatemeh Mamashli, Shayan Abbasi, Jamshid Davoodi, Ali Akbar Saboury
Alteration in permeability of blood-brain barrier (BBB) is of immense importance in the pathogenesis of diabetes-related central nervous system disorders. Considering the presence of glycated insulin in plasma of type 2 diabetic patients, we hypothesized that glycated insulin could induce changes in paracellular permeability in BBB. Therefore, the authors decided to study the effect of glycated insulin on paracellular permeability in a BBB model. Furthermore, the change induced in insulin conformation upon glycation was studied...
February 11, 2018: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29443345/disulphide-bond-exchange-inhibited-by-air-kinetic-and-thermodynamic-products-in-a-library-of-macrocyclic-cysteine-derivatives
#15
Agnieszka Cholewiak, Łukasz Dobrzycki, Janusz Jurczak, Filip Ulatowski
In this paper we present the synthesis and reactivity of dithiols comprising of two cysteine moieties attached to a dipicolinic acid core. Oxidation of these thiols provides oligomeric macrocycles. Monomers with 13-membered rings are kinetic products which are, however, strained and readily transform into higher oligomers under basic conditions or elevated temperature via a disulphide exchange reaction. Dimers, which are the most stable thermodynamic products, equilibrate only under inert conditions with thiolate as a catalyst...
February 14, 2018: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/29442516/engineering-nitroxide-functional-surfaces-using-bioinspired-adhesion
#16
Hendrik Woehlk, Jan Steinkoenig, Christiane Lang, Lukas Michalek, Vanessa Trouillet, Peter Krolla-Sidenstein, Anja S Goldmann, Leonie Barner, James P Blinco, Christopher Barner-Kowollik, Kathryn E Fairfull-Smith
We pioneer a versatile surface modification strategy based on mussel inspired oxidative catecholamine polymerization for the design of nitroxide containing thin polymer films. A 3,4-dihydroxy-L-phenylalanine (L-DOPA) monomer equipped with a 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) derived oxidation-labile hydroxylamine functional group is employed as a universal coating agent to generate polymer scaffolds with persistent radical character. Various types of materials including silicon, titanium, ceramic alumina and inert poly(tetrafluoroethylene) (PTFE) were successfully coated with poly(DOPA-TEMPO) thin films in a one-step dip-coating procedure under aerobic, slightly alkaline (pH 8...
February 14, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29442498/proteostasis-and-the-regulation-of-intra-and-extracellular-protein-aggregation-by-atp-independent-molecular-chaperones-lens-%C3%AE-crystallins-and-milk-caseins
#17
John A Carver, Heath Ecroyd, Roger J W Truscott, David C Thorn, Carl Holt
Molecular chaperone proteins perform a diversity of roles inside and outside the cell. One of the most important is the stabilization of misfolding proteins to prevent their aggregation, a process that is potentially detrimental to cell viability. Diseases such as Alzheimer's, Parkinson's, and cataract are characterized by the accumulation of protein aggregates. In vivo, many proteins are metastable and therefore under mild destabilizing conditions have an inherent tendency to misfold, aggregate, and hence lose functionality...
February 14, 2018: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29440986/reconsideration-of-amyloid-hypothesis-and-tau-hypothesis-in-alzheimer-s-disease
#18
REVIEW
Fuyuki Kametani, Masato Hasegawa
The so-called amyloid hypothesis, that the accumulation and deposition of oligomeric or fibrillar amyloid β (Aβ) peptide is the primary cause of Alzheimer's disease (AD), has been the mainstream concept underlying AD research for over 20 years. However, all attempts to develop Aβ-targeting drugs to treat AD have ended in failure. Here, we review recent findings indicating that the main factor underlying the development and progression of AD is tau, not Aβ, and we describe the deficiencies of the amyloid hypothesis that have supported the emergence of this idea...
2018: Frontiers in Neuroscience
https://www.readbyqxmd.com/read/29440708/differential-role-of-fl-bid-and-t-bid-during-verotoxin-1-induced-apoptosis-in-burkitt-s-lymphoma-cells
#19
Justine Debernardi, Emilie Hollville, Marc Lipinski, Joëlle Wiels, Aude Robert
The globotriaosylceramide Gb3 is a glycosphingolipid expressed on a subpopulation of germinal center B lymphocytes which has been recognized as the B cell differentiation antigen CD77. Among tumoral cell types, Gb3/CD77 is strongly expressed in Burkitt's lymphoma (BL) cells as well as other solid tumors including breast, testicular and ovarian carcinomas. One known ligand of Gb3/CD77 is Verotoxin-1 (VT-1), a Shiga toxin produced in specific E. coli strains. Previously, we have reported that in BL cells, VT-1 induces apoptosis via a caspase-dependent and mitochondria-dependent pathway...
February 14, 2018: Oncogene
https://www.readbyqxmd.com/read/29437575/saturated-fatty-acids-undergo-intracellular-crystallization-and-activate-the-nlrp3-inflammasome-in-macrophages
#20
Tadayoshi Karasawa, Akira Kawashima, Fumitake Usui-Kawanishi, Sachiko Watanabe, Hiroaki Kimura, Ryo Kamata, Koumei Shirasuna, Yutaro Koyama, Ayana Sato-Tomita, Takashi Matsuzaka, Hiroshi Tomoda, Sam-Yong Park, Naoya Shibayama, Hitoshi Shimano, Tadashi Kasahara, Masafumi Takahashi
OBJECTIVE: Inflammation provoked by the imbalance of fatty acid composition, such as excess saturated fatty acids (SFAs), is implicated in the development of metabolic diseases. Recent investigations suggest the possible role of the NLRP3 (nucleotide-binding oligomerization domain, leucine-rich repeat and pyrin domain containing 3) inflammasome, which regulates IL-1β (interleukin 1β) release and leads to inflammation, in this process. Therefore, we investigated the underlying mechanism by which SFAs trigger NLRP3 inflammasome activation...
February 8, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
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