Saidbakhrom Saidjalolov, Emmanuelle Braud, Zainab Edoo, Laura Iannazzo, Filippo Rusconi, Margaux Riomet, Antoine Sallustrau, Frédéric Taran, Michel Arthur, Matthieu Fonvielle, Mélanie Etheve-Quelquejeu
β-Lactams, the cornerstone of antibiotherapy, inhibit multiple and partially redundant targets referred to as transpeptidases or penicillin-binding proteins. These enzymes catalyze the essential cross-linking step of the polymerization of cell wall peptidoglycan. The understanding of the mechanisms of action of β-lactams and of resistance to these drugs requires the development of reliable methods to characterize their targets. Here, we describe an activity-based purification method of β-lactam targets based on click and release chemistry...
May 17, 2021: Chemistry: a European Journal