keyword
MENU ▼
Read by QxMD icon Read
search

Exosite

keyword
https://www.readbyqxmd.com/read/27909054/identification-of-a-substrate-selective-exosite-within-the-metalloproteinase-anthrax-lethal-factor
#1
Allison B Goldberg, Eunice Cho, Chad J Miller, Hua Jane Lou, Benjamin E Turk
The metalloproteinase anthrax lethal factor (LF) is secreted by Bacillus anthracis to promote disease virulence through disruption of host signaling pathways. LF is a highly specific protease, exclusively cleaving mitogen-activated protein kinase kinases (MKKs) and rodent NACHT leucine-rich repeat and pyrin domain containing protein 1B (NLRP1B). How LF achieves such restricted substrate specificity is not understood. Previous studies have suggested the existence of an exosite interaction between LF and MKKs that promotes cleavage efficiency and specificity...
December 1, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/27904902/a-novel-hirudin-derivative-inhibiting-thrombin-without-bleeding-for-subcutaneous-injection
#2
Bing Zhao, Yanling Zhang, Yinong Huang, Jinchao Yu, Yaran Li, Qi Wang, Yixin Ma, Hou-Yan Song, Min Yu, Wei Mo
Currently, anticoagulants would be used to prevent thrombosis. Thrombin is an effector enzyme for haemostasis and thrombosis. We designed a direct thrombin inhibitor peptide (DTIP) using molecular simulation and homology modelling and demonstrated that the C-terminus of DTIP interacts with exosite I, and N-terminus with the activity site of thrombin, respectively. DTIP interfered with thrombin-mediated coagulation in human, rat and mouse plasma (n=10 per group) and blocked clotting in human whole blood in vitro...
December 1, 2016: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/27899589/through-bond-effects-in-the-ternary-complexes-of-thrombin-sandwiched-by-two-dna-aptamers
#3
Andrea Pica, Irene Russo Krauss, Valeria Parente, Hisae Tateishi-Karimata, Satoru Nagatoishi, Kouhei Tsumoto, Naoki Sugimoto, Filomena Sica
Aptamers directed against human thrombin can selectively bind to two different exosites on the protein surface. The simultaneous use of two DNA aptamers, HD1 and HD22, directed to exosite I and exosite II respectively, is a very powerful approach to exploit their combined affinity. Indeed, strategies to link HD1 and HD22 together have been proposed in order to create a single bivalent molecule with an enhanced ability to control thrombin activity. In this work, the crystal structures of two ternary complexes, in which thrombin is sandwiched between two DNA aptamers, are presented and discussed...
November 28, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27794633/molecular-dynamics-simulations-of-aptamer-binding-reveal-generalized-allostery-in-thrombin
#4
Jiajie Xiao, Freddie R Salsbury
Thrombin is an attractive target for antithrombotic therapy due to its central role in thrombosis and hemostasis as well as its role in inducing tumor growth, metastasis and tumor invasion. The thrombin-binding DNA aptamer (TBA), is under investigation for anticoagulant drugs. Although aptamer binding experiments have been revealed various effects on thrombin's enzymatic activities, the detailed picture of the thrombin's allostery from TBA binding is still unclear. To investigate thrombin's response to the aptamer-binding at the molecular level, we compare the mechanical properties and free energy landscapes of the free and aptamer-bound thrombin using microsecond-scale all-atom GPU-based molecular dynamics simulations...
October 31, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27767076/direct-and-indirect-mechanisms-of-klk4-inhibition-revealed-by-structure-and-dynamics
#5
Blake T Riley, Olga Ilyichova, Mauricio G S Costa, Benjamin T Porebski, Simon J de Veer, Joakim E Swedberg, Itamar Kass, Jonathan M Harris, David E Hoke, Ashley M Buckle
The kallikrein-related peptidase (KLK) family of proteases is involved in many aspects of human health and disease. One member of this family, KLK4, has been implicated in cancer development and metastasis. Understanding mechanisms of inactivation are critical to developing selective KLK4 inhibitors. We have determined the X-ray crystal structures of KLK4 in complex with both sunflower trypsin inhibitor-1 (SFTI-1) and a rationally designed SFTI-1 derivative to atomic (~1 Å) resolution, as well as with bound nickel...
October 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27760842/molecular-mapping-of-%C3%AE-thrombin-%C3%AE-t-%C3%AE-2-glycoprotein-i-%C3%AE-2gpi-interaction-reveals-how-%C3%AE-2gpi-affects-%C3%AE-t-functions
#6
Laura Acquasaliente, Daniele Peterle, Simone Tescari, Nicola Pozzi, Vittorio Pengo, Vincenzo De Filippis
: β2-Glycoprotein I (β2GpI) is the major autoantigen in the antiphospholipid syndrome, a thrombotic autoimmune disease. Nonetheless, the physiological role of β2GpI is still unclear. In a recent work, we have shown that β2GpI selectively inhibits the procoagulant functions of human a-thrombin (αT) (i.e. prolongs fibrin clotting time, tc, and inhibits αT-induced platelets aggregation) without affecting the unique anticoagulant activity of the protease, i.e. the proteolytic generation of the anticoagulant protein C (PC) from the PC zymogen, which interacts with αT exclusively at the protease catalytic site...
October 19, 2016: Biochemical Journal
https://www.readbyqxmd.com/read/27722252/picolinic-acids-as-%C3%AE-exosite-inhibitors-of-botulinum-neurotoxin-a-light-chain
#7
Paul T Bremer, Song Xue, Kim D Janda
In developing small-molecule inhibitors of botulinum neurotoxin serotype A light chain (BoNT/A LC), substituted picolinic acids were identified. Extensive investigation into the SAR of the picolinic acid scaffold revealed 5-(1-butyl-4-chloro-1H-indol-2-yl)picolinic acid (CBIP), which possessed low micromolar activity against BoNT/A. Kinetic and docking studies demonstrated binding of CBIP to the β-exosite: a largely unexplored site on the LC that holds therapeutic relevance for botulism treatment.
October 13, 2016: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/27678152/in-silico-prediction-of-the-molecular-basis-of-cltx-and-aactx-interaction-with-matrix-metalloproteinase-2-mmp-2-to-inhibit-glioma-cell-invasion
#8
Houcemeddine Othman, Silke Andrea Wieninger, Mohamed ElAyeb, Michael Nilges, Najet Srairi-Abid
Glioblastoma is the deadliest type of brain cancer. Treatment could target the Matrix metalloproteinase-2 (MMP-2), which is known to be involved in the invasion process of glioblastoma cells. But current available inhibitors are not selective to MMP-2 due to their interaction with the catalytic binding site, which is highly conserved in all MMPs structures. Interestingly, members of the chloride channel blocker scorpion toxins, such as chlorotoxin (ClTx) and AaCTx, inhibit glioblastoma cell invasion and show a promising therapeutic potential...
September 28, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27624125/thrombin-cleavage-of-plasmodium-falciparum-erythrocyte-membrane-protein-1-inhibits-cytoadherence
#9
Mark R Gillrie, Bernard Renaux, Eleanor Russell-Goldman, Marion Avril, Andrew J Brazier, Koichiro Mihara, Enrico Di Cera, Danny A Milner, Morley D Hollenberg, Joseph D Smith, May Ho
UNLABELLED: Plasmodium falciparum malaria remains one of the most deadly infections worldwide. The pathogenesis of the infection results from the sequestration of infected erythrocytes (IRBC) in vital organs, including the brain, with resulting impairment of blood flow, hypoxia, and lactic acidosis. Sequestration occurs through the adhesion of IRBC to host receptors on microvascular endothelium by Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1), a large family of variant surface antigens, each with up to seven extracellular domains that can bind to multiple host receptors...
September 13, 2016: MBio
https://www.readbyqxmd.com/read/27579517/multiple-allosteric-sites-are-involved-in-the-modulation-of-insulin-degrading-enzyme-activity-by-somatostatin
#10
Grazia R Tundo, Elena Di Muzio, Chiara Ciaccio, Diego Sbardella, Donato Di Pierro, Fabio Polticelli, Massimo Coletta, Stefano Marini
Somatostatin is a cyclic peptide, released in the gastrointestinal system and in the central nervous system, where it is involved in the regulation of cognitive and sensory functions, motor activity and sleep. It is a substrate of the Insulin-Degrading Enzyme (IDE) as well as a modulator of its activity and expression. In this paper, we have investigated the modulatory role of somatostatin on IDE activity at 37°C and pH 7.3 for various substrates (namely Insulin, Aβ1-40 and Bradykinin), in order to quantitatively characterize the correlation between the specific features of the substrates and the regulatory mechanism...
August 31, 2016: FEBS Journal
https://www.readbyqxmd.com/read/27576863/allosteric-activation-of-senp1-by-sumo1-%C3%AE-grasp-domain-involves-a-dock-and-coalesce-mechanism
#11
Jingjing Guo, Huan-Xiang Zhou
Small ubiquitin-related modifiers (SUMOs) are conjugated to proteins to regulate a variety of cellular processes. SENPs are cysteine proteases with a catalytic center located within a channel between two subdomains that catalyzes SUMO C-terminal cleavage for processing of SUMO precursors and de-SUMOylation of target proteins. The β-grasp domain of SUMOs binds to an exosite cleft, and allosterically activates SENPs via an unknown mechanism. Our molecular dynamics simulations showed that binding of the β-grasp domain induces significant conformational and dynamic changes in SENP1, including widening of the exosite cleft and quenching of nanosecond dynamics in all but a distal region...
2016: ELife
https://www.readbyqxmd.com/read/27497176/detecting-allosteric-networks-using-molecular-dynamics-simulation
#12
S Bowerman, J Wereszczynski
Allosteric networks allow enzymes to transmit information and regulate their catalytic activities over vast distances. In principle, molecular dynamics (MD) simulations can be used to reveal the mechanisms that underlie this phenomenon; in practice, it can be difficult to discern allosteric signals from MD trajectories. Here, we describe how MD simulations can be analyzed to reveal correlated motions and allosteric networks, and provide an example of their use on the coagulation enzyme thrombin. Methods are discussed for calculating residue-pair correlations from atomic fluctuations and mutual information, which can be combined with contact information to identify allosteric networks and to dynamically cluster a system into highly correlated communities...
2016: Methods in Enzymology
https://www.readbyqxmd.com/read/27474452/direct-inhibition-of-matrix-metalloproteinase-2-mmp-2-by-epigallocatechin-3-gallate-a-possible-role-for-the-fibronectin-type-ii-repeats
#13
Shalinee Jha, Shankar Prasad Kanaujia, Anil M Limaye
Matrix metalloproteinases (MMPs) -2 and -9, also called gelatinases, constitute a distinct subgroup within the MMP family of extracellular matrix remodeling proteases. Gelatinases are implicated in tumor cell invasion and metastasis, and are attractive therapeutic targets. Several synthetic small molecule inhibitors of MMPs developed till date have failed in clinical trials. This has prompted explorations into the gamut of dietary compounds and nutraceuticals for specific inhibitors of MMPs with desirable properties...
November 15, 2016: Gene
https://www.readbyqxmd.com/read/27460023/considerations-on-inhibition-approaches-for-proinflammatory-functions-of-adam-proteases
#14
Daniela Dreymueller, Andreas Ludwig
Proteases of the disintegrin and metalloproteinase (ADAM) family mediate the proteolytic shedding of various surface molecules including cytokine precursors, adhesion molecules, growth factors, and receptors. Within the vasculature ADAM10 and ADAM17 regulate endothelial permeability, transendothelial leukocyte migration, and the adhesion of leukocytes and platelets. In vivo studies show that both proteases are implicated in several inflammatory pathologies, for example, edema formation, leukocyte infiltration, and thrombosis...
July 26, 2016: Platelets
https://www.readbyqxmd.com/read/27362887/functional-characterization-of-selective-exosite-binding-inhibitors-of-matrix-metalloproteinase-13-mmp-13-experimental-validation-in-human-breast-and-colon-cancer
#15
Roopa Kothapalli, Kodappully Sivaraman Siveen, Tuan Zea Tan, Jean Paul Thiery, Alan Prem Kumar, Gautam Sethi, Kunchithapadam Swaminathan
Considering the pathological significance of MMP-13 in breast and colon cancers, exosite-based inhibition of the C-terminal hemopexin (Hpx) domain could serve as an alternative strategy to develop selective inhibitors for MMP-13.Two of six lead compounds, compound 5 (2,3-dihydro-1,4-benzodioxine-5-carboxylic acid) and compound 6 (1-acetyl-4-hydroxypyrrolidine-2-carboxylic acid) exhibited considerable inhibitory activity against MMP-13. Complementing to this study, we have also shown the gene expression levels of MMP-13 within the subtypes of colon and breast cancers classified from patients' tissue samples to provide a better understanding on which subtype of breast cancer patients would get benefited by MMP-13 inhibitors...
June 29, 2016: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/27338096/polyphosphate-is-a-novel-cofactor-for-regulation-of-complement-by-a-serpin-c1-inhibitor
#16
Lakshmi C Wijeyewickrema, Emilie Lameignere, Lilian Hor, Renee C Duncan, Toshikazu Shiba, Richard J Travers, Piyushkumar R Kapopara, Victor Lei, Stephanie A Smith, Hugh Kim, James H Morrissey, Robert N Pike, Edward M Conway
The complement system plays a key role in innate immunity, inflammation, and coagulation. The system is delicately balanced by negative regulatory mechanisms that modulate the host response to pathogen invasion and injury. The serpin, C1-esterase inhibitor (C1-INH), is the only known plasma inhibitor of C1s, the initiating serine protease of the classical pathway of complement. Like other serpin-protease partners, C1-INH interaction with C1s is accelerated by polyanions such as heparin. Polyphosphate (polyP) is a naturally occurring polyanion with effects on coagulation and complement...
September 29, 2016: Blood
https://www.readbyqxmd.com/read/27305147/dabigatran-and-argatroban-diametrically-modulate-thrombin-exosite-function
#17
Calvin H Yeh, Alan R Stafford, Beverly A Leslie, James C Fredenburgh, Jeffrey I Weitz
Thrombin is a highly plastic molecule whose activity and specificity are regulated by exosites 1 and 2, positively-charged domains that flank the active site. Exosite binding by substrates and cofactors regulates thrombin activity by localizing thrombin, guiding substrates, and by inducing allosteric changes at the active site. Although inter-exosite and exosite-to-active-site allostery have been demonstrated, the impact of active site ligation on exosite function has not been examined. To address this gap, we used surface plasmon resonance to determine the effects of dabigatran and argatroban, active site-directed inhibitors, on thrombin binding to immobilized γA/γA-fibrin or glycoprotein Ibα peptide via exosite 1 and 2, respectively, and thrombin binding to γA/γ'-fibrin or factor Va, which is mediated by both exosites...
2016: PloS One
https://www.readbyqxmd.com/read/27262193/effect-of-thrombin-inhibitors-on-positive-feedback-in-the-coagulation-cascade
#18
N B Ustinov, E G Zav'yalova, A M Kopylov
The coagulation cascade is a series of sequential reactions of limited proteolysis of protein factors resulting in generation of thrombin. Thrombin mediates both positive and negative feedback in regulating this cascade by taking part in activation of several factors. Some thrombin inhibitors, by affecting positive feedback, inhibit generation of thrombin itself. In the current study, we used two thrombin inhibitors: argatroban, a low molecular weight reversible competitive inhibitor that binds to the active site, and bivalirudin, a bivalent oligopeptide that blocks the active site and binding center of protein substrates (exosite I)...
March 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27179421/exploiting-the-antithrombotic-effect-of-the-pro-thrombin-inhibitor-bothrojaracin
#19
Mariane Assafim, Flávia S Frattani, Marcos S Ferreira, Dione M Silva, Robson Q Monteiro, Russolina B Zingali
Bothrojaracin is a 27 kDa C-type lectin-like protein from Bothrops jararaca snake venom. It behaves as a potent thrombin inhibitor upon high-affinity binding to thrombin exosites. Bothrojaracin also forms a stable complex with prothrombin that can be detected in human plasma. Formation of the zymogen-inhibitor complex severely decreases prothrombin activation and contributes to the anticoagulant activity of bothrojaracin. In the present study, we employed two rodent models to evaluate the antithrombotic effect of bothrojaracin in vivo: stasis-induced thrombosis and thrombin-induced pulmonary thromboembolism...
September 1, 2016: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/27159247/cation-coordination-alters-the-conformation-of-a-thrombin-binding-g-quadruplex-dna-aptamer-that-affects-inhibition-of-thrombin
#20
Elena Zavyalova, Grigory Tagiltsev, Roman Reshetnikov, Alexander Arutyunyan, Alexey Kopylov
Thrombin-binding aptamers are promising anticoagulants. HD1 is a monomolecular antiparallel G-quadruplex with two G-quartets linked by three loops. Aptamer-thrombin interactions are mediated with two TT-loops that bind thrombin exosite I. Several cations were shown to be coordinated inside the G-quadruplex, including K(+), Na(+), NH4(+), Ba(2+), and Sr(2+); on the contrary, Mn(2+) was coordinated in the grooves, outside the G-quadruplex. K(+) or Na(+) coordination provides aptamer functional activity. The effect of other cations on aptamer functional activity has not yet been described, because of a lack of relevant tests...
May 9, 2016: Nucleic Acid Therapeutics
keyword
keyword
49519
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"