keyword
MENU ▼
Read by QxMD icon Read
search

Enhancement

keyword
https://www.readbyqxmd.com/read/28535663/up-regulation-of-rank-expression-via-erk1-2-by-insulin-contributes-to-the-enhancement-of-osteoclast-differentiation
#1
Ju Hee Oh, Na Kyung Lee
Despite the importance of the receptor activator of nuclear factor (NF)-kappaB ligand (RANKL)-RANK signaling mechanisms on osteoclast differentiation, little has been studied on how RANK expression is regulated or what regulates its expression during osteoclastogenesis. We show here that insulin signaling increases RANK expression, thus enhancing osteoclast differentiation by RANKL. Insulin stimulation induced RANK gene expression in time- and dose-dependent manners and insulin receptor shRNA completely abolished RANK expression induced by insulin in bone marrow-derived monocyte/ macrophage cells (BMMs)...
May 22, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28535660/-clinical-investigation-on-ct-guided-cryoablation-for-treating-invasive-chest-wall-or-pleural-tumors
#2
M Wang, Y W Pan, Z G Zhou, Y Cui, K P Du, S Li
Objective: To evaluate the feasibility and short-term effect of CT guided cryoablation for malignant chest wall or pleural involvement. Methods: To follow up 22 patients with chest wall or pleural involvement of various malignancies who underwent cryoablation from January 2012 to January 2015 by conducting postoperative 1-, 3- and 6-month enhanced CT, MRI or PET-CT examinations, respectively, evaluate local curative effect, and observe their progression-free survival (PFS), postoperative pain remission and complications after the cryoablation...
May 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28535650/-texture-analysis-based-on-contrast-enhanced-mri-can-predict-treatment-response-to-neoadjuvant-chemotherapy-of-breast-cancer
#3
S H Sun, C W Zhou, L Y Zhao, R Z Zhang, H Ouyang
Objective: To investigate whether texture analysis based on contrast-enhanced MRI can predict pathological complete response of locally advanced breast cancer undergoing neoadjuvant chemotherapy(NAC). Methods: Forty-seven patients with breast cancer undergone neoadjuvant chemotherapy from January 2015 to February 2016 were divided into pathological complete response (pCR) group or non-pathological complete response (non-pCR) group based on surgical pathology. Their parameters of texture analysis based on MRI before neoadjuvant chemotherapy and after 2 cycles of treatment were analyzed...
May 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28535649/-cancer-associated-fibroblasts-regulate-the-chemoresistance-of-lung-cancer-cell-line-a549-via-sdf-1-secretion
#4
F Zou, Z H Zhang, Y T Zhang, J Q Zhao, X L Zhang, C L Wen, X Y Song, W M Zhou
Objective: To investigate whether cancer-associated- fibroblasts (CAF), the key component of tumor microenvironment, regulate the chemoresistant capacity of lung cancer cell line A549 through SDF-1 secretion. Methods: Primary cell isolation techniques was used to isolate cancer-associated-fibroblasts from lung cancer patients. MTT assay was applied to determine the proliferation and chemoresistance of A549 cells. Quantative PCR was used to detect the mRNA changes of Bcl-xL. Western blotting was used to detect the protein expression of Bcl-xL...
May 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28535648/-effect-of-%C3%AE-40p53-isoform-on-enhancing-the-pro-apoptotic-function-of-p53-in-tumor-cells
#5
B S Wang, H W Zhao, L X Qiao, J Q Shan, Q S Hou, D X Chen, H L Guo
Objective: To investigate the effect of Δ40p53, an alternative spliced isoform of p53 lacking the N-ter minus, on the pro-apoptotic function of p53. Methods: The wild-type p53 was ectopically expressed in HCT116-p53(-/-) (endogenous Δ40p53 expression), HCT116-p53(+ /+) (wild-type p53) and H1299 (p53-null) cells by adenoviral delivery, while Δ40p53 plasmid were transfected into these cells to overexpress Δ40p53. The levels of Δ40p53 and p53 mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR) and quantitative PCR...
May 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28535640/-study-of-diffusion-weighted-imaging-in-combination-with-conventional-mri-signs-for-inflammation-activity-of-chronic-hepatitis-b
#6
H W Ren, H Y Ye, W M An, J H Dong, Y Liu
Objective: To study the correlation between ADC values of diffusion weighted imaging (DWI) and the pathological grading in inflammation activity of chronic hepatitis B, and combined with conventional MRI features to predict the diagnosis effectiveness. Methods: A total of 142 cases of patients with chronic hepatitis B were selected as inflammatory group in 302 Hospital of PLA from January 2014 to December 2015, while 20 cases of healthy subjects without history of liver disease were chosen as control group...
May 23, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28535613/expression-and-purification-of-biologically-active-human-bone-morphogenetic-protein-4-in-recombinant-chinese-hamster-ovary-cells
#7
Minyub Cha, Nara Han, Jia Pi, Yongsu Jeong, Kwanghee Baek, Jaeseung Yoon
Bone morphogenetic protein-4 (BMP-4) is considered to have therapeutic potential for various diseases including cancers; however, the high expression of biologically active recombinant human BMP-4 (rhBMP-4) needed to manufacture rhBMP-4 for therapeutic purposes has yet to be established. In the current study, we established a recombinant Chinese hamster ovary (rCHO) cell line overexpressing rhBMP-4 as well as a production process using 7.5-l bioreactor (5 l working volume). The expression of the mature rhBMP-4 was significantly enhanced by recombinant furin expression...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28535609/synthesis-of-%C3%AE-galactooligosaccharide-using-bifidobacterial-%C3%AE-galactosidase-purified-from-recombinant-escherichia-coli
#8
So Young Oh, So Youn Youn, Myung Soo Park, Hyoung Geun Kim, Nam In Baek, Zhi Peng Li, Geun Eog Ji
Galactooligosaccharides (GOS) are known to be selectively utilized by Bifidobacterium, which can bring about healthy changes of the composition of intestinal microflora. In this study, β-GOS are synthesized using bifidobacterial β-galactosidase(G1) purified from recombinant E. coli with a high GOS yield and with high productivity and enhanced bifidogenic activity. The purified recombinant G1 showed maximum production of β-GOS at pH 8.5 and 45°C. A matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis of the major peaks of the produced β-GOS showed MW of 527 and 689, indicating the synthesis of β-GOS at degrees of polymerization (DP) of 3 and DP4, respectively...
May 24, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28535604/use-of-transcatheter-contrast-enhanced-ultrasound-with-vessel-navigation-to-reduce-the-contrast-requirement-during-transarterial-chemoembolization-in-patients-with-renal-impairment
#9
https://www.readbyqxmd.com/read/28535596/leaf-litter-leachate-concentration-promotes-heterotrophy-in-freshwater-biofilms-understanding-consequences-of-water-scarcity
#10
Aingeru Martínez, John Stephen Kominoski, Aitor Larrañaga
Climate change is increasing overall temporal variability in precipitation resulting in a seasonal water availability, both increasing periods of flooding and water scarcity. During low water availability periods, the concentration of leachates from riparian vegetation increases, subsequently increasing dissolved organic matter (DOM). Moreover, shifts in riparian vegetation by land use changes impact the quantity and quality of DOM. Our objective was to test effects of increasing DOM concentrations from Eucalyptus grandis (one of the most cultivated tree species in the world) leachates on the metabolism (respiration, R; gross primary productivity, GPP) and extracellular enzyme activities (EEAs) of freshwater biofilms...
May 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28535595/enhancement-of-carbon-and-nitrogen-removal-by-helophytes-along-subsurface-water-flowpaths-receiving-treated-wastewater
#11
Miquel Ribot, Susana Bernal, Myrto Nikolakopoulou, Timothy N Vaessen, Joaquín Cochero, Esperança Gacia, Albert Sorolla, Alba Argerich, Francesc Sabater, Manel Isnard, Eugènia Martí
Wastewater treatment plant (WWTP) effluents are sources of dissolved organic carbon (DOC) and inorganic nitrogen (DIN) to receiving streams, which can eventually become saturated by excess of DIN. Aquatic plants (i.e., helophytes) can modify subsurface water flowpaths as well as assimilate nutrients and enhance microbial activity in the rhizosphere, yet their ability to increase DIN transformation and removal in WWTP-influenced streams is poorly understood. We examined the influence of helophytes on DIN removal along subsurface water flowpaths and how this was associated with DOC removal and labile C availability...
May 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28535590/emission-of-volatile-organic-compounds-and-production-of-secondary-organic-aerosol-from-stir-frying-spices
#12
Tengyu Liu, Qianyun Liu, Zijun Li, Lei Huo, ManNin Chan, Xue Li, Zhen Zhou, Chak K Chan
Cooking is an important source of volatile organic compounds (VOCs) and a potential source of secondary organic aerosol (SOA) both indoors and outdoors. In this study, VOC emissions from heating corn oil and stir-frying spices (i.e. garlic, ginger, myrcia and zanthoxylum piperitum (Sichuan pepper)) were characterized using an on-line membrane inlet vacuum ultraviolet single-photon ionization time-of-flight mass spectrometer (VUV-SPI-TOFMS). VOC emissions from heating corn oil were dominated by aldehydes, which were enhanced by factors of one order of magnitude when stir-frying spices...
May 19, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28535545/portuguese-recommendations-for-the-use-of-methotrexate-in-rheumatic-diseases-2016-update
#13
Ana Catarina Duarte, Daniela Santos-Faria, Maria João Gonçalves, Alexandre Sepriano, Ana Filipa Mourão, Cátia Duarte, Joana Sousa Neves, Ana Filipa Águeda, Pedro Ávila Ribeiro, Alexandra Daniel, Adriano Neto, Ana Cordeiro, Ana Rodrigues, Anabela Barcelos, Cândida Silva, Cristina Ponte, Elsa Vieira-Sousa, Filipa Teixeira, Filipa Oliveira-Ramos, Filipe Araújo, Filipe Barcelos, Helena Canhão, Helena Santos, João Ramos, Joaquim Pereira, José Costa, José António Melo Gomes, Luís Cunha-Miranda, Lúcia Costa, Marcos Cerqueira, Margarida Cruz, Maria José Santos, Miguel Bernardes, Paula Oliveira, Pedro Abreu, Ricardo Figueira, Rita Barros, Sandra Falcão, Patrícia Pinto, Sofia Pimenta, Susana Capela, Vitor Teixeira, João Eurico Fonseca
BACKGROUND: Methotrexate (MTX) is the first-line drug in the treatment of rheumatoid arthritis (RA) and the most commonly prescribed disease modifying anti-rheumatic drug. Moreover, it is also used as an adjuvant drug in patients under biologic therapies, enhancing the efficacy of biologic agents. OBJECTIVES: To review the literature and update the Portuguese recommendations for the use of MTX in rheumatic diseases first published in 2009. METHODS: The first Portuguese guidelines for the use of MTX in rheumatic diseases were published in 2009 and were integrated in the multinational 3E Initiative (Evidence Expertise Exchange) project...
May 9, 2017: Acta Reumatológica Portuguesa
https://www.readbyqxmd.com/read/28535537/triggering-of-suicidal-erythrocyte-death-by-exemestane
#14
Abdulla Al Mamun Bhuyan, Rosi Bissinger, Hang Cao, Florian Lang
BACKGROUND/AIMS: The steroidal aromatase inactivator exemestane blocks estrogen biosynthesis and is thus employed for the prevention and treatment of breast cancer. Exemestane is in part effective by stimulation of suicidal cell death or apoptosis. Side effects of exemestane treatment include anemia. At least in theory, exemestane induced anemia could be secondary to stimulation of suicidal erythrocyte death or eryptosis, characterized by cell shrinkage and cell membrane scrambling with phosphatidylserine translocation to the erythrocyte surface...
May 11, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28535525/expanded-hemodialysis-a-new-therapy-for-a-new-class-of-membranes
#15
Claudio Ronco, Gaetano La Manna
A wide spectrum of molecules is retained in end-stage kidney disease, normally defined as uremic toxins. These solutes have different molecular weights and radii. Current dialysis membranes and techniques only remove solutes in the range of 50-15,000 Da, with limited or no capability to remove solutes in the middle to high molecular weight range (up to 50,000 Da). Improved removal has been obtained with high cut-off (HCO) membranes, with albumin loss representing a limitation to their practical application...
2017: Contributions to Nephrology
https://www.readbyqxmd.com/read/28535517/coupled-plasma-filtration-adsorption-application-for-liver-and-thyroid-toxins
#16
Gabriele Donati, Irene Capelli, Anna Laura Croci Chiocchini, Nicolò Natali, Anna Scrivo, Gaetano La Manna
Coupled plasma filtration and adsorption (CPFA) is a detoxification system that combines a plasma adsorption circuit and a continuous renal replacement therapy circuit. Its main application is for sepsis and septic shock with or without acute renal failure. Several recent studies have suggested that CPFA can reduce the mortality when the volume of plasma absorbed on the styrenic resin is at least >0.18 L/kg/day. At present, new applications for CPFA are under investigation, also in patients without significant kidney failure...
2017: Contributions to Nephrology
https://www.readbyqxmd.com/read/28535513/pkc-%C3%AE-triggers-egfr-ubiquitination-endocytosis-and-erk-activation-in-podocytes-stimulated-with-high-glucose
#17
Chun-Tao Lei, Yan-Hong Wei, Hui Tang, Qian Wen, Chen Ye, Chun Zhang, Hua Su
BACKGROUND: Protein Kinase C-α (PKC-α) and epidermal growth factor receptor (EGFR) are both involved in diabetic kidney disease; however, the connection between these two proteins during high glucose-induced podocyte injury remains uncertain. METHODS: Diabetes was induced in SD rats by streptozotocin (STZ). Fourteen days later, the kidney cortex was removed and subjected to plasma membrane isolation and lipid raft fractionation. In vitro study human podocyte cell line was differentiated and subjected to various treatments...
May 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28535511/capn4-enhances-osteopontin-expression-through-activation-of-the-wnt-%C3%AE-catenin-pathway-to-promote-epithelial-ovarian-carcinoma-metastasis
#18
Xiaoming Yang, Jing Sun, Dandan Xia, Xupei Can, Lei Liu, Jing Zhang, Huali Xu, Na Du, Wenxue Liu, Fangqian Shen, Zhenbo Zhang, Yunyan Sun, Xiaowei Xi
BACKGROUND AND AIM: Increasing evidence shows that the calpain regulatory subunit Capn4 can modulate the proliferation and metastasis of cancer cells, and plays an important role in the development of malignant tumors. However, there is no information on the clinical significance of Capn4 in epithelial ovarian carcinoma (EOC) or the molecular mechanisms by which Capn4 promotes the growth and metastasis of EOC. Therefore, the aim of this study was to clarify the role of Capn4 in EOC. METHODS: We evaluated Capn4 and osteopontin (OPN) expression in EOC cell lines and tissues from patients with ovarian cancer by western blotting and immunohistochemical analysis...
May 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28535499/the-role-of-5-htr6-in-mossy-fiber-sprouting-activating-fyn-and-p-erk1-2-in-pilocarpine-induced-chronic-epileptic-rats
#19
Wanhui Lin, Wenli Huang, Shenggen Chen, Mingxing Lin, Qingyu Huang, Huapin Huang
OBJECTIVE: Our primary objective is to verify whether 5-HTR6 is involved in the development of mossy fiber sprouting (MFS), and to determine how the progression of MFS is affected by 5-HTR6. METHODS: A total of 90 male adult Sprague-Dawley rats were allocated into either the control group (n=36) or the epileptic group (n=54). Status epilepticus (SE) of rats was induced by the intraperitoneal (i.p.) injection of LiCl-pilocarpine. We conducted our experiments in two stages...
May 25, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28535491/efficient-bioconversion-of-high-content-volatile-fatty-acids-into-microbial-lipids-by-cryptococcus-curvatus-atcc-20509
#20
Jia'nan Liu, Xiangfeng Huang, Rui Chen, Ming Yuan, Jia Liu
The lower utilization ratios of high-content VFAs resulted in less lipid yield by the oleaginous yeast Cryptococcus curvatus ATCC 20509. In this work, increasing the inoculation concentration to OD600=5.1 improved the acetic acid utilization ratio (99.8%) and lipids production (4.63g/L) in mediums with 30g/L of acetic acid. For the higher acetic acid concentration (40g/L), increasing the nitrogen to carbon ratio (0.033) and raising the initial pH (pH=8) was superior over improvement in the inoculate, with the lipid production increased from 1...
April 21, 2017: Bioresource Technology
keyword
keyword
49496
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"