keyword
MENU ▼
Read by QxMD icon Read
search

Lcn2

keyword
https://www.readbyqxmd.com/read/28070126/lipocalin-2-protects-the-brain-during-inflammatory-conditions
#1
S S Kang, Y Ren, C-C Liu, A Kurti, K E Baker, G Bu, Y Asmann, J D Fryer
Sepsis is a prevalent health issue that can lead to central nervous system (CNS) inflammation with long-term behavioral and cognitive alterations. Using unbiased proteomic profiling of over 100 different cytokines, we found that Lipocalin-2 (LCN2) was the most substantially elevated protein in the CNS after peripheral administration of lipopolysaccharide (LPS). To determine whether the high level of LCN2 in the CNS is protective or deleterious, we challenged Lcn2(-/-) mice with peripheral LPS and determined effects on behavior and neuroinflammation...
January 10, 2017: Molecular Psychiatry
https://www.readbyqxmd.com/read/28062797/targeted-proteomics-for-multiplexed-verification-of-markers-of-colorectal-tumorigenesis
#2
Anuli Christiana Uzozie, Nathalie Selevsek, Asa Wahlander, Paolo Nanni, Jonas Grossmann, Achim Weber, Federico Buffoli, Giancarlo Marra
Targeted proteomic methods can accelerate the verification of multiple tumor marker candidates in large series of patient samples. We utilized the targeted approach known as selected / multiple reaction monitoring (SRM/MRM) to verify potential protein markers of colorectal adenoma identified by our group in previous transcriptomic and quantitative shotgun proteomic studies of a large cohort of precancerous colorectal lesions. We developed SRM assays to reproducibly detect and quantify 25 (62.5%) of the 40 selected proteins in an independent series of precancerous and cancerous tissue samples (19 adenoma/normal mucosa pairs; 17 adenocarcinoma/normal mucosa pairs)...
January 4, 2017: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/28058580/dcf1-triggers-dendritic-spine-formation-and-facilitates-memory-acquisition
#3
Qiang Liu, Ruili Feng, Yu Chen, Guanghong Luo, Huang Yan, Ling Chen, Rongfei Lin, Yuqiang Ding, Tieqiao Wen
Dendritic spines, a special kind of structure in nerve cells, play a key role in performing cellular function. Structural abnormalities of the dendritic spine may contribute to synaptic dysfunction and have been implicated in memory formation. However, the molecular mechanisms that trigger dendritic spine loss remain unclear. Here, we show that the absence of dendritic cell factor 1 (Dcf1) appeared dendritic spines dysplasia, which in turn leads to the damage of learning and memory; in contrast, enhancing Dcf1 expression rescues dendritic spines morphology and function, indicating a pivotal role of Dcf1 in cellular function...
January 5, 2017: Molecular Neurobiology
https://www.readbyqxmd.com/read/28013112/urine-interleukin-18-and-lipocalin-2-are-biomarkers-of-acute-tubular-necrosis-in-patients-with-cirrhosis-a-systematic-review-and-meta-analysis
#4
Jeremy Puthumana, Xavier Ariza, Justin M Belcher, Isabel Graupera, Pere Ginès, Chirag R Parikh
BACKGROUND & AIMS: Acute kidney injury (AKI) is a common complication in patients with cirrhosis that increases mortality. The most common causes of AKI in these patients are prerenal azotemia, acute tubular necrosis (ATN), and hepatorenal syndrome; it is important to determine the etiology of AKI to select the proper treatment and predict patient outcome. Urine biomarkers could be used to differentiate between patients with ATN and functional causes of AKI. We performed a systemic review and meta-analysis of published studies to determine whether urine levels of interleukin 18 (IL18) and lipocalin 2 (LCN2 or NGAL) associate with development of ATN in patients with cirrhosis...
December 21, 2016: Clinical Gastroenterology and Hepatology
https://www.readbyqxmd.com/read/28004388/lcn2-overexpression-in-bone-enhances-the-hematopoietic-compartment-via-modulation-of-the-bone-marrow-microenvironment
#5
Delfina Costa, Elisa Principi, Edoardo Lazzarini, Fiorella Descalzi, Ranieri Cancedda, Patrizio Castagnola, Sara Tavella
Lipocalin-2 (LCN2) is a member of the lipocalin family whose expression is modulated in several conditions, including cell differentiation, innate immunity, stress and cancer. Although it is known that it is expressed in bone, its function in this tissue remains poorly studied. To this end, we took advantage of transgenic mice lines that expressed LCN2 driven by a bone specific type I collagen (LCN2-Tg). In the bone marrow (BM) of LCN2-Tg mice we observed an increased number of phenotypically long-term hematopoietic stem cells (LT-HSC) that also displayed a higher proliferation rate compared to wild type controls (Wt)...
December 22, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27999286/testosterone-deficiency-induces-changes-of-the-transcriptomes-of-visceral-adipose-tissue-in-miniature-pigs-fed-a-high-fat-and-high-cholesterol-diet
#6
Lifan Zhang, Yueqin Cai, Shengjuan Wei, Yun Ling, Liang Zhu, Dongfeng Li, Zhaowei Cai
Testosterone deficiency causes fat deposition, particularly in visceral fat, and its replacement might reverse fat accumulation, however, the underlying mechanisms of such processes under diet-induced adiposity are largely unknown. To gain insights into the genome-wide role of androgen on visceral adipose tissue (VAT), RNA-Seq was used to investigate testosterone deficiency induced changes of VAT in miniature pigs fed a high-fat and high-cholesterol (HFC) diet among intact male pigs (IM), castrated male pigs (CM), and castrated male pigs with testosterone replacement (CMT) treatments...
December 16, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27983637/limited-link-between-oxidative-stress-and-ochratoxin-a-induced-renal-injury-in-an-acute-toxicity-rat-model
#7
Liye Zhu, Tao Yu, Xiaozhe Qi, Jing Gao, Kunlun Huang, Xiaoyun He, Haoshu Luo, Wentao Xu
Ochratoxin A (OTA) displays nephrotoxicity and hepatotoxicity. However, in the acute toxicity rat model, there is no evidence on the relationship between OTA and nephrotoxicity and hepatotoxicity. Based on this, the integrated analysis of physiological status, damage biomarkers, oxidative stress, and DNA damage were performed. After OTA treatment, the body weight decreased and AST, ALP, TP, and BUN levels in serum increased. Hydropic degeneration, swelling, vacuolization, and partial drop occurred in proximal tubule epithelial cells...
December 14, 2016: Toxins
https://www.readbyqxmd.com/read/27980236/expression-of-uterine-lipocalin-2-and-its-receptor-during-early-to-mid-pregnancy-period-in-mares
#8
Shingo Haneda, Kentaro Nagaoka, Yasuo Nambo, Masato Kikuchi, Yasuko Nakano, Junyou Li, Motozumi Matsui, Yo-Ichi Miyake, Kazuhiko Imakawa
From previous cDNA subtraction studies analyzing gene expression in equine endometrium, high lipocalin 2 (LCN2) mRNA expression was found in the gravid endometrium. In the uterus, LCN2 may transport hydrophobic molecules and siderophores with iron, or may form a complex with another protein, however, the expression of uterine LCN2 beyond day 20 of equine pregnancy and its receptor has not been characterized. To study the expression and potential roles of uterine LCN2 from pre-implantation to mid-gestation period, stage-specific endometrial samples were obtained from day 13 (day 0 = ovulation) cyclic and days 13, 19, 25, and 60 to 131 pregnant mares...
December 16, 2016: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/27912767/lipocalin2-suppresses-metastasis-of-colorectal-cancer-by-attenuating-nf-%C3%AE%C2%BAb-dependent-activation-of-snail-and-epithelial-mesenchymal-transition
#9
Meibao Feng, Jieqiong Feng, Wuzhen Chen, Wubin Wang, Xuesong Wu, Jing Zhang, Fangying Xu, Maode Lai
BACKGROUND: Lipocalin2 (LCN2) is a secretory protein that is aberrantly expressed in several types of cancer and has been involved in metastatic progression. However, neither mechanisms nor the role that LCN2 plays in the metastasis of colorectal cancer are clear. METHODS: LCN2 expression in colorectal cancer was detected by immunohistochemistry in 400 tissue specimens and Kaplan-Meier survival analysis was performed. In vitro, real-time PCR, western blot, colony formation assay, immunofluorescence assay, wound healing assay, migration and invasion experiment were performed to investigate the effects of LCN2 in epithelial mesenchymal transition (EMT), migration and invasion, respectively...
December 3, 2016: Molecular Cancer
https://www.readbyqxmd.com/read/27902979/altered-systemic-adipokines-in-patients-with-chronic-urticaria
#10
Hoang Kim Tu Trinh, Duy Le Pham, Ga-Young Ban, Hyun-Young Lee, Hae-Sim Park, Young-Min Ye
BACKGROUND: Increasing evidence suggests that adipokines affect immune responses and chronic urticaria (CU) is associated with an altered immune response related to chronic systemic inflammation. Our objectives were to investigate whether adipokines are involved in CU pathogenesis and to outline relationships between adipokines and urticaria severity and quality of life. METHODS: Serum adiponectin, leptin, lipocalin-2 (LCN2), interleukin (IL)-10, IL-6, and tumor necrosis factor (TNF)-α concentrations were measured by enzyme-linked immunosorbent assays in 191 CU patients and 89 healthy controls...
2016: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/27888128/neutrophil-gelatinase-associated-lipocalin-production-negatively-correlates-with-hk-2-cell-impairment-evaluation-of-ngal-as-a-marker-of-toxicity-in-hk-2-cells
#11
Martina Hauschke, Erika Roušarová, Pavel Flídr, Jan Čapek, Antonín Libra, Tomáš Roušar
Neutrophil gelatinase-associated lipocalin is an extracellular protein produced mostly in kidney. Recently, it has become a promising biomarker of renal damage in vivo. On the other hand, the validation of NGAL as a biomarker for nephrotoxicity estimation in vitro has not been characterized in detail yet. Since the HK-2 cells are frequently used human kidney cell line, we aimed to characterize the production of NGAL in these cells and to evaluate NGAL as a possible marker of cell impairment. We used heavy metals (mercury, cadmium), peroxide, drugs (acetaminophen, gentamicin) and cisplatin to mimic nephrotoxicity...
March 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/27869162/the-histone-demethylase-kdm4b-regulates-peritoneal-seeding-of-ovarian-cancer
#12
C Wilson, L Qiu, Y Hong, T Karnik, G Tadros, B Mau, T Ma, Y Mu, J New, R J Louie, S Gunewardena, A K Godwin, O W Tawfik, J Chien, K F Roby, A J Krieg
Epithelial ovarian cancer (EOC) has poor prognosis and rapid recurrence because of widespread dissemination of peritoneal metastases at diagnosis. Multiple pathways contribute to the aggressiveness of ovarian cancer, including hypoxic signaling mechanisms. In this study, we have determined that the hypoxia-inducible histone demethylase KDM4B is expressed in ∼60% of EOC tumors assayed, including primary and matched metastatic tumors. Expression of KDM4B in tumors is positively correlated with expression of the tumor hypoxia marker CA-IX, and is robustly induced in EOC cell lines exposed to hypoxia...
November 21, 2016: Oncogene
https://www.readbyqxmd.com/read/27825391/oestrous-cycle-dependent-equine-uterine-immune-response-to-induced-infectious-endometritis
#13
Christina D Marth, Simon M Firestone, Lisa Y Glenton, Glenn F Browning, Neil D Young, Natali Krekeler
Infectious endometritis is a major cause of reduced pregnancy rates in horses. The objectives of this study were to establish a timeline of the innate immune response in the uterus of healthy horses and to investigate the oestrous cycle effect on this. Endometrial biopsies were collected from five horses before and at 3, 12, 24, 48 and 72 h after inoculation of Escherichia coli, once in oestrus and once in dioestrus. They were analysed by quantitative real-time PCR, microbiology and histology. Neutrophil numbers increased from very low levels in the absence of inflammation to severe neutrophilia 3 h after inoculation...
November 8, 2016: Veterinary Research
https://www.readbyqxmd.com/read/27808277/the-influence-of-warm-ischemia-elimination-on-kidney-injury-during-transplantation-clinical-and-molecular-study
#14
Dorota Kamińska, Katarzyna Kościelska-Kasprzak, Paweł Chudoba, Agnieszka Hałoń, Oktawia Mazanowska, Agnieszka Gomółkiewicz, Piotr Dzięgiel, Dominika Drulis-Fajdasz, Marta Myszka, Agnieszka Lepiesza, Wojciech Polak, Maria Boratyńska, Marian Klinger
Kidney surface cooling was used during implantation to assess the effect of warm ischemia elimination on allograft function, histological changes and immune-related gene expression. 23 recipients were randomly assigned to a group operated on with kidney surface cooling during implantation (ice bag technique, IBT group), and the other 23 recipients receiving the contralateral kidney from the same donor were operated on with a standard technique. Three consecutive kidney core biopsies were obtained during the transplantation procedure: after organ recovery, after cold ischemia and after reperfusion...
November 3, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27800610/lipocalin-2-ngal-attenuates-autophagy-to-exacerbate-cardiac-apoptosis-induced-by-myocardial-ischemia
#15
Hye Kyoung Sung, Yee Kwan Chan, Meng Han, James Won Suk Jahng, Erfei Song, Danielson Eric, Thorsten Berger, Tak W Mak, Gary Sweeney
Lipocalin-2 (Lcn2; also termed neutrophil gelatinase-associated lipocalin (NGAL)) levels correlate positively with heart failure (HF) yet mechanisms via which Lcn2 contributes to the pathogenesis of HF remian unclear. In this study we used coronary artery ligation surgery to induce ischemia in wild type (wt) mice and this induced a significant increase in myocardial Lcn2. We then compared wt and Lcn2 knockout (KO) mice and observed that wt mice showed greater ischemia-induced caspase-3 activation and DNA damage measured by TUNEL than Lcn2KO mice...
November 1, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27796299/disposal-of-iron-by-a-mutant-form-of-lipocalin-2
#16
Jonathan Barasch, Maria Hollmen, Rong Deng, Eldad A Hod, Peter B Rupert, Rebecca J Abergel, Benjamin E Allred, Katherine Xu, Shaun F Darrah, Yared Tekabe, Alan Perlstein, Rebecca Wax, Efrat Bruck, Jacob Stauber, Kaitlyn A Corbin, Charles Buchen, Vesna Slavkovich, Joseph Graziano, Steven L Spitalnik, Guanhu Bao, Roland K Strong, Andong Qiu
Iron overload damages many organs. Unfortunately, therapeutic iron chelators also have undesired toxicity and may deliver iron to microbes. Here we show that a mutant form (K3Cys) of endogenous lipocalin 2 (LCN2) is filtered by the kidney but can bypass sites of megalin-dependent recapture, resulting in urinary excretion. Because K3Cys maintains recognition of its cognate ligand, the iron siderophore enterochelin, this protein can capture and transport iron even in the acidic conditions of urine. Mutant LCN2 strips iron from transferrin and citrate, and delivers it into the urine...
October 31, 2016: Nature Communications
https://www.readbyqxmd.com/read/27793136/activation-of-innate-immune-genes-in-caprine-blood-leukocytes-after-systemic-endotoxin-challenge
#17
Øyvind Salvesen, Malin R Reiten, Peter M H Heegaard, Michael A Tranulis, Arild Espenes, Kerstin Skovgaard, Cecilie Ersdal
BACKGROUND: Sepsis is a serious health problem associated with a range of infectious diseases in animals and humans. Early events of this syndrome can be mimicked by experimental administration of lipopolysaccharides (LPS). Compared with mice, small ruminants and humans are highly sensitive to LPS, making goats valuable in inflammatory models. We performed a longitudinal study in eight Norwegian dairy goats that received LPS (0.1 μg/kg, Escherichia coli O26:B6) intravenously. A control group of five goats received corresponding volumes of sterile saline...
October 28, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27782193/lipocalin-2-lcn2-is-a-promising-target-for-cholangiocarcinoma-treatment-and-bile-lcn2-level-is-a-potential-cholangiocarcinoma-diagnostic-marker
#18
Kun-Chun Chiang, Ta-Sen Yeh, Ren-Chin Wu, Jong-Hwei S Pang, Chi-Tung Cheng, Shang-Yu Wang, Horng-Heng Juang, Chun-Nan Yeh
Cholangiocarcinoma (CCA) is a devastating disease due to resistance to traditional chemotherapies and radiotherapies. New therapeutic strategies against CCA are urgently needed. This study investigated the role of lipocalin-2 (LCN2) in human cholangiocarcinoma as a potential therapeutic target and diagnostic marker. So far, the role of LCN2 in cancer is still controversial and studies regarding the role of LCN2 in CCA are limited. LCN2 knockdown inhibited CCA cell growth in vitro and in vivo through induction of cell cycle arrest at G0/G1 phases and decreased metastatic potential due to repression of epithelial-mesenchymal transition (EMT)...
October 26, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27734904/lipocalin-2-prevents-intestinal-inflammation-by-enhancing-phagocytic-bacterial-clearance-in-macrophages
#19
Takahiko Toyonaga, Minoru Matsuura, Kiyoshi Mori, Yusuke Honzawa, Naoki Minami, Satoshi Yamada, Taku Kobayashi, Toshifumi Hibi, Hiroshi Nakase
Lipocalin 2 (Lcn2), also called neutrophil gelatinase B-associated lipocalin (NGAL), is an anti-microbial peptide originally identified in neutrophil granules. Although Lcn2/NGAL expression is increased in the inflamed intestinal tissues of patients with inflammatory bowel disease, the role of Lcn2/NGAL in the development of intestinal inflammation remains unclear. Here we investigated the role of Lcn2/NGAL in intestinal inflammation using a spontaneous mouse colitis model, interleukin-10 knock out (IL-10 KO) mice...
October 13, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27729871/lipocalin-2-lcn2-expression-in-hepatic-malfunction-and-therapy
#20
Anastasia Asimakopoulou, Sabine Weiskirchen, Ralf Weiskirchen
Lipocalin 2 (LCN2) is a secreted protein that belongs to the Lipocalins, a group of transporters of small lipophilic molecules such as steroids, lipopolysaccharides, iron, and fatty acids in circulation. Two decades after its discovery and after a high variety of published findings, LCN2's altered expression has been assigned to critical roles in several pathological organ conditions, including liver injury and steatosis, renal damage, brain injury, cardiomyopathies, muscle-skeletal disorders, lung infection, and cancer in several organs...
2016: Frontiers in Physiology
keyword
keyword
49444
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"