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https://www.readbyqxmd.com/read/28717313/study-of-morphology-chemical-and-amino-acid-composition-of-red-deer-meat
#1
Eleonora Okuskhanova, Bahytkul Assenova, Maksim Rebezov, Kumarbek Amirkhanov, Zhanibek Yessimbekov, Farida Smolnikova, Almagul Nurgazezova, Gulnur Nurymkhan, Marilyne Stuart
AIM: The aim of this study was to evaluate red deer (maral) meat quality based on chemical composition, pH, water-binding capacity (WBC), and amino acid content. MATERIALS AND METHODS: Maral meat surface morphology measurements were obtained by scanning electron microscopy. Active acidity (pH) was determined by potentiometry. Samples were analyzed for WBC by exudation of moisture to a filter paper by the application of pressure. Chemical composition (moisture, protein, fat, and ash fractions) was obtained by drying at 150°C and by extraction, using ethylic ether, and ashing at 500-600°C...
June 2017: Veterinary World
https://www.readbyqxmd.com/read/28717206/an-amino-acid-signature-associated-with-obesity-predicts-2-year-risk-of-hypertriglyceridemia-in-school-age-children
#2
Sofia Moran-Ramos, Elvira Ocampo-Medina, Ruth Gutierrez-Aguilar, Luis Macías-Kauffer, Hugo Villamil-Ramírez, Blanca E López-Contreras, Paola León-Mimila, Joel Vega-Badillo, Roxana Gutierrez-Vidal, Ricardo Villarruel-Vazquez, Erandi Serrano-Carbajal, Blanca E Del-Río-Navarro, Adriana Huertas-Vázquez, Teresa Villarreal-Molina, Isabel Ibarra-Gonzalez, Marcela Vela-Amieva, Carlos A Aguilar-Salinas, Samuel Canizales-Quinteros
Childhood obesity is associated with a number of metabolic abnormalities leading to increased cardiovascular risk. Metabolites can be useful as early biomarkers and new targets to promote early intervention beginning in school age. Thus, we aimed to identify metabolomic profiles associated with obesity and obesity-related metabolic traits. We used data from the Obesity Research Study for Mexican children (ORSMEC) in Mexico City and included a case control (n = 1120), cross-sectional (n = 554) and a longitudinal study (n = 301) of 6-12-year-old children...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28710429/the-a2v-mutation-as-a-new-tool-for-hindering-a%C3%AE-aggregation-a-neutron-and-x-ray-diffraction-study
#3
Laura Cantu', Laura Colombo, Tatiana Stoilova, Bruno Demé, Hideyo Inouye, Rachel Booth, Valeria Rondelli, Giuseppe Di Fede, Fabrizio Tagliavini, Elena Del Favero, Daniel A Kirschner, Mario Salmona
We have described a novel C-to-T mutation in the APP gene that corresponds to an alanine to valine substitution at position 673 in APP (A673V), or position 2 of the amyloid-β (Aβ) sequence. This mutation is associated with the early onset of AD-type dementia in homozygous individuals, whereas it has a protective effect in the heterozygous state. Correspondingly, we observed differences in the aggregation properties of the wild-type and mutated Aβ peptides and their mixture. We have carried out neutron diffraction (ND) and x-ray diffraction (XRD) experiments on magnetically-oriented fibers of Aβ1-28WT and its variant Aβ1-28A2V...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28706879/multiple-substrate-usage-of-coxiella-burnetii-to-feed-a-bipartite-metabolic-network
#4
Ina Häuslein, Franck Cantet, Sarah Reschke, Fan Chen, Matteo Bonazzi, Wolfgang Eisenreich
The human pathogen Coxiella burnetii causes Q-fever and is classified as a category B bio-weapon. Exploiting the development of the axenic growth medium ACCM-2, we have now used (13)C-labeling experiments and isotopolog profiling to investigate the highly diverse metabolic network of C. burnetii. To this aim, C. burnetii RSA 439 NMII was cultured in ACCM-2 containing 5 mM of either [U-(13)C3]serine, [U-(13)C6]glucose, or [U-(13)C3]glycerol until the late-logarithmic phase. GC/MS-based isotopolog profiling of protein-derived amino acids, methanol-soluble polar metabolites, fatty acids, and cell wall components (e...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28705827/3-hydroxyisobutyrate-dehydrogenase-is-involved-in-valine-and-isoleucine-degradation-in-a-thaliana
#5
Peter Schertl, Lennart Danne, Hans-Peter Braun
In plants, amino acid catabolism is especially relevant at metabolic stress situations, e.g. limited carbohydrate availability during extended darkness. Under these conditions, amino acids are used as alternative substrates for respiration. Complete oxidation of the branched chain amino acids (BCAAs) Leucine, Isoleucine and Valine in the mitochondria efficiently allows formation of ATP by oxidative phosphorylation. However, the metabolic pathways for BCAA breakdown are largely unknown so far in plants. A systematic search for Arabidopsis genes encoding proteins resembling enzymes involved in BCAA catabolism in animals, fungi and bacteria as well as proteomic analyses of mitochondrial fractions from Arabidopsis allowed identification of a putative 3-Hydroxyisobutyrate dehydrogenase, AtHDH1 (At4g20930), involved in Valine degradation...
July 13, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28705621/functionalized-pla-peg-nanoparticles-targeting-intestinal-transporter-pept1-for-oral-delivery-of-acyclovir
#6
Betty Gourdon, Caroline Chemin, Amélie Moreau, Thomas Arnauld, Philippe Baumy, Salvatore Cisternino, Jean-Manuel Péan, Xavier Declèves
Targeting intestinal di- and tri-peptide transporter PepT1 with prodrugs is a successful strategy to improve oral drug bioavailability, as demonstrated with valacyclovir, a prodrug of acyclovir. The aim of this new drug delivery strategy is to over-concentrate a poorly absorbed drug on the intestinal membrane surface by targeting PepT1 with functionalized polymer nanoparticles. In the present study, poly(lactic acid)-poly(ethylene glycol)-ligand (PLA-PEG-ligand) nanoparticles were obtained by nanoprecipitation...
July 10, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/28704533/peripheral-blood-mitochondrial-dna-content-in-relation-to-circulating-metabolites-and-inflammatory-markers-a-population-study
#7
Judita Knez, Vannina G Marrachelli, Nicholas Cauwenberghs, Ellen Winckelmans, Zhenyu Zhang, Lutgarde Thijs, Jana Brguljan-Hitij, Michelle Plusquin, Christian Delles, Daniel Monleon, Josep Redón, Jan A Staessen, Tim S Nawrot, Tatiana Kuznetsova
Mitochondrial DNA (mtDNA) content might undergo significant changes caused by metabolic derangements, oxidative stress and inflammation that lead to development and progression of cardiovascular diseases. We, therefore, investigated in a general population the association of peripheral blood mtDNA content with circulating metabolites and inflammatory markers. We examined 310 subjects (50.6% women; mean age, 53.3 years) randomly selected from a Flemish population. Relative mtDNA content was measured by quantitative real-time PCR in peripheral blood cells...
2017: PloS One
https://www.readbyqxmd.com/read/28703303/terminally-truncated-isopenicillin-n-synthase-generates-a-dithioester-product-evidence-for-a-thioaldehyde-intermediate-and-a-new-mode-of-reaction-for-non-heme-iron-oxidases
#8
Luke McNeill, Toby Brown, Malkit Sami, Ian Clifton, Nicolai Burzlaff, Timothy Claridge, Robert Adlington, Jack Baldwin, Peter J Rutledge, Christopher Schofield
Isopenicillin N synthase (IPNS) catalyses the oxidation of a tripeptide, L-δ-(α-aminoadipoyl)-L-cysteinyl-D-valine (ACV), to isopenicillin N (IPN), the first-formed β-lactam in penicillin biosynthesis. IPNS catalysis is dependent upon an iron(II) cofactor and oxygen as co-substrate. In the absence of substrate, the carbonyl oxygen of the side-chain amide of the penultimate residue, Gln330, co-ordinates to the active site metal. Substrate binding ablates this interaction, triggering rearrangement of seven C-terminal residues which move to take up a conformation that extends the final α-helix and encloses the active site...
July 12, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28697549/deamidation-of-pseudopeptidic-molecular-hydrogelators-and-its-application-to-controlled-release
#9
César A Angulo-Pachón, Diego Navarro-Barreda, Celia M Rueda, Francisco Galindo, Juan F Miravet
HYPOTHESIS: The incorporation of a succinic acid-derived moiety in amino acid derivatives would favor an intramolecular catalysis of a deamidation reaction. Such reaction would permit controlled disassembly of molecular hydrogelators and the use of the hydrogels for controlled release of actives. EXPERIMENTAL: Low molecular weight hydrogelators containing a succinic acid-derived moiety were prepared by conventional organic synthesis procedures. Hydrogels were examined by electron microscopy and (1)HNMR studies were carried out to evaluate the solubility in water of the hydrogelators and the deamidation reaction...
July 3, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/28696790/competitive-inhibition-of-amino-acid-transport-in-human-preovulatory-ovarian-follicles
#10
Maciej Jóźwik, Marcin Jóźwik, Anna Justyna Milewska, Frederick C Battaglia, Michał Jóźwik
To date we have yet to examine whether amino acid (AA) transport in human ovarian follicles is affected by competitive inhibition. In contrast, transplacental transfer of AAs in late-gestation sheep is characterized by reciprocal competition. This phenomenon has been described by algebraic equations of umbilical uptake of AAs based on maternal arterial concentrations. In the present translational study at a university teaching hospital, we verified whether these equations apply to the transport of AAs from blood to follicular fluid (FF) in human preovulatory follicles...
July 11, 2017: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/28696673/how-nothing-boosts-affinity-hydrophobic-ligand-binding-to-the-virtually-vacated-s1-pocket-of-thermolysin
#11
Stefan G Krimmer, Jonathan Cramer, Johannes Schiebel, Andreas Heine, Gerhard Klebe
We investigated the hydration state of the deep, well accessible hydrophobic S1' specificity pocket of the metalloprotease thermolysin with purposefully designed ligands using high-resolution crystallography and isothermal titration calorimetry. The S1' pocket is known to recognize selectively a very stringent set of aliphatic side chains such as valine, leucine and isoleucine of putative substrates. We engineered a weak-binding ligand covering the active site of the protease without addressing the S1' pocket, thus transforming it into an enclosed cavity...
July 11, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28695583/quantitative-13-c-isotope-labelling-based-analysis-to-elucidate-the-influence-of-environmental-parameters-on-the-production-of-fermentative-aromas-during-wine-fermentation
#12
Stéphanie Rollero, Jean-Roch Mouret, Audrey Bloem, Isabelle Sanchez, Anne Ortiz-Julien, Jean-Marie Sablayrolles, Sylvie Dequin, Carole Camarasa
Nitrogen and lipids are key nutrients of grape must that influence the production of fermentative aromas by wine yeast, and we have previously shown that a strong interaction exists between these two nutrients. However, more than 90% of the acids and higher alcohols (and their acetate ester derivatives) were derived from intermediates produced by the carbon central metabolism (CCM). The objective of this study was to determine how variations in nitrogen and lipid resources can modulate the contribution of nitrogen and carbon metabolisms for the production of fermentative aromas...
July 11, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28692538/metabolic-syndrome-and-obesity-are-the-cornerstones-of-liver-fibrosis-in-hiv-monoinfected-patients-results-of-the-metafib-study
#13
Maud Lemoine, Karine Lacombe, Jean Philippe Bastard, Manuela Sébire, Laurent Fonquernie, Nadia Valin, Soraya Fellahi, Jacqueline Capeau, Pierre-Marie Girard, Jean-Luc Meynard
BACKGROUND: Metabolic syndrome (MetS) and nonalcoholic fatty liver disease (NAFLD) have become a common finding in HIV-infected patients. However, the severity, risk factors and pathogenesis of liver fibrosis in this population have been poorly documented. OBJECTIVES: to assess the impact of MetS on liver fibrosis and analyze the association between MetS, liver fibrosis and markers of adipose tissue and macrophage activation. METHODS: In a matched cohort of HIV-1-monoinfected patients with and without MetS, after exclusion of other causes of liver disease, we assessed liver stiffness measurement (LSM) and measured levels of serum adipokines, HOMA index and soluble CD163 and CD14 as markers of fat, insulin resistance and macrophage/monocyte activation, respectively...
July 7, 2017: AIDS
https://www.readbyqxmd.com/read/28691814/intervention-of-dietary-dipeptide-gamma-l-glutamyl-l-valine-%C3%AE-ev-ameliorates-inflammatory-response-in-a-mouse-model-of-lps-induced-sepsis
#14
MacKenzie E Chee, Kaustav Majumder, Yoshinori Mine
Sepsis, the systemic inflammatory response syndrome (SIRS) with infection is one of the leading causes of death in critically ill patients in the developed world due to the lack of effective antisepsis treatments. This study examined the efficacy of dietary dipeptide gamma-l-glutamyl-l-valine (γ-EV), which was characterized previously as an anti-inflammatory peptide, in an LPS-induced mouse model of sepsis. BALB/c mice were administered γ-EV via oral gavage followed by an intraperitoneal injection of LPS to induce sepsis...
July 10, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28691070/molecular-local-and-network-level-basis-for-the-enhanced-stiffness-of-hydrogel-networks-formed-from-coassembled-racemic-peptides-predictions-from-pauling-and-corey
#15
Katelyn Nagy-Smith, Peter J Beltramo, Eric Moore, Robert Tycko, Eric M Furst, Joel P Schneider
Hydrogels prepared from self-assembling peptides are promising materials for medical applications, and using both l- and d-peptide isomers in a gel's formulation provides an intuitive way to control the proteolytic degradation of an implanted material. In the course of developing gels for delivery applications, we discovered that a racemic mixture of the mirror-image β-hairpin peptides, named MAX1 and DMAX1, provides a fibrillar hydrogel that is four times more rigid than gels formed by either peptide alone-a puzzling observation...
June 28, 2017: ACS Central Science
https://www.readbyqxmd.com/read/28690380/identification-of-inhibitors-against-%C3%AE-isopropylmalate-synthase-of-mycobacterium-tuberculosis-using-docking-mm-pbsa-hybrid-approach
#16
Preeti Pandey, Andrew M Lynn, Pradipta Bandyopadhyay
α-Isopropylmalate Synthase (α-IPMS) encoded by leuA in Mycobacterium tuberculosis (M.tb) is involved in the leucine biosynthesis pathway and is extremely critical for the synthesis of branched-chain amino acids (leucine, isoleucine and valine). α-IPMS activity is required not only for the proliferation of M.tb but is also indispensable for its survival during the latent phase of infection. It is absent in humans and is widely regarded as one of the validated drug targets against Tuberculosis (TB). Despite its essentiality, any study on designing of potential chemical inhibitors against α-IPMS has not been reported so far...
2017: Bioinformation
https://www.readbyqxmd.com/read/28682414/a-practical-approach-to-asymmetric-synthesis-of-dolastatin-10
#17
Wen Zhou, Xiao-Di Nie, Yu Zhang, Chang-Mei Si, Zhu Zhou, Xun Sun, Bang-Guo Wei
Dolastatin 10, an antineoplastic agent for cancer chemotherapy, is a linear peptide possessing N,N-dimethyl Val-OH, l-valine, (3R,4S,5S)-dolaisoleucine, (2R,3R,4S)-dolaproine and (S)-dolaphenine. Our efficient synthesis includes the following three key features: (1) SmI2-induced cross-coupling was employed to couple aldehyde 11 with (S)-N-tert-butanesulfinyl imine 12 to generate the required stereocenters of Dap (7); (2) asymmetric addition of chiral N-sulfinyl imine 10 provided a straightforward approach to the synthesis of the protected Doe ((S,S)-8); (3) a practical method to the key subunit Val-Dil (24a) has been established as an alternative synthetic route for the synthesis of this challenging chemical structure...
July 6, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28678685/expression-of-wild-type-or-g1862t-mutant-hbe-antigen-of-subgenotype-a1-of-hepatitis-b-virus-and-the-unfolded-protein-response-in-huh7-cells
#18
Nimisha Harshadrai Bhoola, Anna Kramvis
The G1862T mutation, which occurs most frequently in subgenotype A1 of the hepatitis B virus (HBV), results in a valine to phenylalanine substitution at the -3 position of the signal peptide cleavage site at the amino end of the precore/core (preC/C) precursor protein. The objective of this study was to functionally characterize the G1862T mutation relative to its wild-type counterpart in subgenotype A1. Huh7 cells were transfected with subgenotype A1 replication-competent plasmids, with and without G1862T...
June 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28676720/a-channelopathy-mutation-in-the-voltage-sensor-discloses-contributions-of-a-conserved-phenylalanine-to-gating-properties-of-kv1-1-channels-and-ataxia
#19
Sonia Hasan, Cecilia Bove, Gabriella Silvestri, Elide Mantuano, Anna Modoni, Liana Veneziano, Lara Macchioni, Therese Hunter, Gary Hunter, Mauro Pessia, Maria Cristina D'Adamo
Channelopathy mutations prove informative on disease causing mechanisms and channel gating dynamics. We have identified a novel heterozygous mutation in the KCNA1 gene of a young proband displaying typical signs and symptoms of Episodic Ataxia type 1 (EA1). This mutation is in the S4 helix of the voltage-sensing domain and results in the substitution of the highly conserved phenylalanine 303 by valine (p.F303V). The contributions of F303 towards K(+) channel voltage gating are unclear and here have been assessed biophysically and by performing structural analysis using rat Kv1...
July 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28675044/delivery-of-chemotherapeutic-vcmmae-using-tobacco-mosaic-virus-nanoparticles
#20
Daniel L Kernan, Amy M Wen, Andrzej S Pitek, Nicole F Steinmetz
The first-line treatment for non-Hodgkin's lymphoma is chemotherapy. While generally well tolerated, off-target effects and chemotherapy-associated complications are still of concern. To overcome the challenges associated with systemic chemotherapy, we developed a biology-inspired, nanoparticle drug delivery system (nanoDDS) making use of the nucleoprotein components of the tobacco mosaic virus (TMV). Virus-based nanoparticles, including the high-aspect ratio soft nanorods formed by TMV, are growing in popularity as nanoDDS due to their simple genetic and chemical engineerability, size and shape tunability, and biocompatibility...
January 1, 2017: Experimental Biology and Medicine
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