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Fengping Chen, Qian Zhou, Jing Xi, Dong-Liang Li, Guido Schnabel, Jiasui Zhan
BACKGROUND: Metalaxyl is a phenylamide fungicide, introduced for oomycete management in the 1970s. RPA190 gene has been reported to be associated with metalaxyl resistance, although other genes may be involved in the process as well. The present study assessed the evolutionary association of amino acid (AA) mutations in RPA190 with metalaxyl resistance in Phytophthora infestans. RESULTS: All isolates from Yunnan were resistant to metalaxyl with multiple AA mutations in RPA190...
February 19, 2018: Pest Management Science
E Ceyhan, M Canbek, M Uyanoglu, A Ozen, B Durmus Ozen, B Berber, A Ozmen Yaylaci, L Resulzade
BACKGROUND: Although some amino acids are recognized to have favorable effects on the liver regeneration after partial hepatectomy (PH), molecular mechanisms underlying these effects are barely known. OBJECTIVE: Our study was aimed to investigate the effects of valine, glutamine, and leucine amino acids on PH-induced NF-κB signal pathway. The research team studied Leucine in a rat model in vivo. The study took place in the medical and surgical experimental research center at the Eskisehir Osmangazi University in Eskisehir, Turkey...
2018: Bratislavské Lekárske Listy
Sébastien Acket, Anthony Degournay, Mathilde Gosset, Franck Merlier, Manual Adrian Troncoso-Ponce, Brigitte Thomasset
In context of fluxomic studies, 13 C labeling analysis of amino acids are very important for solving the carbon flow calculation, because they are synthesized in various biosynthesis pathways and cellular compartments in plant cells. Traditionally, 13 C labeling analysis are performed using low resolution mass spectrometry detector by GC-MS. We developed a method using capillary electrophoresis-high resolution mass spectrometry without derivatization and with better accuracy assessment of labeling measurements comparing to classical GC-MS...
February 13, 2018: Analytical Biochemistry
Xiaoyan Liu, Xiangming Cheng, Xiang Liu, Lu He, Wenli Zhang, Yajie Wang, Wei Sun, Zhigang Ji
Urine metabolomics have been used to identify biomarkers for clinical diseases. However, inter-individual variations and effect factors need to be further evaluated. In the present study, we explored the urine metabolome in a cohort of 203 health adults, 6 patients with benign bladder lesions, and 53 patients with bladder cancer (BCa) using liquid chromatography coupled with high resolution mass spectrometry. Inter-individual analysis of both healthy controls and bladder cancer patients showed that the urine metabolome was relatively stable...
February 16, 2018: International Journal of Cancer. Journal International du Cancer
Behnaz Pezeshkpoor, Katrin J Czogalla, Michael Caspers, Ann-Cristin Berkemeier, Kerstin Liphardt, Suvoshree Ghosh, Marco Kellner, Silvia Ulrich, Anna Pavlova, Johannes Oldenburg
One of the most common and unwanted side effects during oral anticoagulant therapy (OAT) is bleeding complications. In rare cases, vitamin K antagonist (VKA)-related bleeding events are associated with mutations affecting the F9 propeptide at amino acid position 37 due to a substitution of alanine to either valine or threonine. Based on our actual cohort of 18 patients, we update the knowledge on this rare phenotype and its origin. A founder mutation for both variants was reconfirmed by haplotype analysis of intronic and extragenic short tandem repeat (STR) polymorphisms with a higher prevalence in Switzerland than in other regions of Europe...
February 15, 2018: Annals of Hematology
Stéphanie Leruez, Thomas Bresson, Juan M Chao de la Barca, Alexandre Marill, Grégoire de Saint Martin, Adrien Buisset, Jeanne Muller, Lydie Tessier, Cédric Gadras, Christophe Verny, Patrizia Amati-Bonneau, Guy Lenaers, Philippe Gohier, Dominique Bonneau, Gilles Simard, Dan Milea, Vincent Procaccio, Pascal Reynier
Purpose: To determine the plasma metabolomic signature of the exfoliative syndrome (XFS), the most common cause worldwide of secondary open-angle glaucoma. Methods: We performed a targeted metabolomic study, using the standardized p180 Biocrates Absolute IDQ p180 kit with a QTRAP 5500 mass spectrometer, to compare the metabolomic profiles of plasma from individuals with XFS (n = 16), and an age- and sex-matched control group with cataract (n = 18). Results: A total of 151 metabolites were detected correctly, 16 of which allowed for construction of an OPLS-DA model with a good predictive capability (Q2cum = 0...
February 1, 2018: Investigative Ophthalmology & Visual Science
M Zacchigna, F Cateni, G Procida, D Zampieri, M G Mamolo
Methoxy polyethylene glycol conjugated with coenzyme Q10 (mPEG)-CoQ10 and analog adducts with amino acids as spacers were synthesized as a new drug delivery systems for CoQ10. Alanine and branched chain amino acids (valine, leucine and isoleucine) were conjugated to mPEG by an amide linkage and to CoQ10 by an ester bond. Recently, branched chain amino acids (BCAAs), which are released along with CoQ10, have received increasing attention as 'anti-fatigue' elements. FT-IR and 1H NMR spectroscopic analysis were useful to characterize the synthesized conjugates...
December 1, 2017: Die Pharmazie
Lisanne J M Kempkes, Georgia C Boles, Jonathan Martens, Giel Berden, Peter B Armentrout, Jos Oomens
Peptide deamidation of asparaginyl residues is a spontaneous post-translational modification that is believed to play a role in aging and several diseases. It is also a well-known small-molecule loss channel in the MS/MS spectra of protonated peptides. Here we investigate the deamidation reaction, as well as other decomposition pathways, of the protonated dipeptide asparagine-valine ([AsnVal+H] + ) upon low-energy activation in a mass spectrometer. Using a combination of infrared ion spectroscopy, guided ion beam tandem mass spectrometry, and theoretical calculations, we have been able to identify product ion structures and determine the energetics and mechanisms for decomposition...
February 13, 2018: Journal of Physical Chemistry. A
Hui-Juan Li, Wen-Xing Li, Shao-Xing Dai, Yi-Cheng Guo, Jun-Juan Zheng, Jia-Qian Liu, Qian Wang, Bi-Wen Chen, Gong-Hua Li, Jing-Fei Huang
The lack of early diagnostic markers and novel therapeutic targets for clear cell renal cell carcinoma (ccRCC) negatively affects patient prognosis. Cancer metabolism is an attractive area for the understanding of the molecular mechanism of carcinogenesis. The present study attempted to identify metabolic changes from the view of the expression of metabolism-associated genes between control samples and those of ccRCC at different disease stages. Data concerning ccRCC gene expression obtained by RNA-sequencing was obtained from The Cancer Genome Atlas and data on metabolism-associated genes were extracted using the Recon2 model...
February 2018: Oncology Letters
Edjane R Dos Santos, Angelica E Graminha, Mario S Schultz, Isabel Correia, Heloisa S Selistre-de-Araújo, Rodrigo S Corrêa, Javier Ellena, Elisângela de Paula S Lacerda, João Costa Pessoa, Alzir A Batista
Thirteen new ruthenium amino acid complexes were synthesized and characterized. They were obtained by the reaction of α-amino acids (AA) with [RuCl2(P-P)(N-N)], where P-P=1,4-bis(diphenylphosphino)butane (dppb) or 1,3-bis(diphenylphosphino)propane (dppp) and N-N=4,4'-dimethyl-2,2'-bipyridine (4'-Mebipy), 5,5'-dimethyl-2,2'-bipyridine (5'-Mebipy) or 4,4'-Methoxy-2-2'-bipyridine (4'-MeObipy). This afforded a family of complexes formulated as [Ru(AA-H)(P-P)(N-N)]PF6, where AA=glycine (Gly), L-alanine (Ala), L-valine (Val), L-tyrosine (Tyr), L-tryptophan (Trp), L-histidine (His) and L-methionine (Met)...
December 23, 2017: Journal of Inorganic Biochemistry
Justyna Wolak-Dinsmore, Eke G Gruppen, Irina Shalaurova, Steven P Matyus, Russell P Grant, Ray Gegen, Stephan J L Bakker, James D Otvos, Margery A Connelly, Robin P F Dullaart
OBJECTIVES: Plasma branched-chain amino acid (BCAA) levels, measured on nuclear magnetic resonance (NMR) metabolomics research platforms or by mass spectrometry, have been shown to be associated with type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD). We developed a new test for quantification of BCAA on a clinical NMR analyzer and used this test to determine the clinical correlates of BCAA in 2 independent cohorts. DESIGN AND METHODS: The performance of the NMR-based BCAA assay was evaluated...
February 9, 2018: Clinical Biochemistry
Rachana Ohri, Sunil Bhakta, Aimee Fourie-O'Donohue, Josefa Dela Cruz-Chuh, Siao Ping Tsai, Ryan Cook, Binqing Wei, Carl Ng, Athena W Wong, Aaron B Bos, Farzam Farahi, Jiten Bhakta, Thomas H Pillow, Helga Raab, Richard Vandlen, Paul Polakis, Yichin Liu, Hans Erickson, Jagath R Junutula, Katherine R Kozak
THIOMAB antibody technology utilizes cysteine residues engineered onto an antibody to allow for site-specific conjugation. The technology has enabled the exploration of different attachment sites on the antibody in combination with small molecules, peptides, or proteins to yield antibody conjugates with unique properties. As reported previously ( Shen , B. Q. , et al. ( 2012 ) Nat. Biotechnol. 30 , 184 - 189 ; Pillow , T. H. , et al. ( 2017 ) Chem. Sci. 8 , 366 - 370 ), the specific location of the site of conjugation on an antibody can impact the stability of the linkage to the engineered cysteine for both thio-succinimide and disulfide bonds...
February 9, 2018: Bioconjugate Chemistry
Pooja Phull, Vaishali Sanchorawala, Lawreen H Connors, Gheorghe Doros, Frederick L Ruberg, John L Berk, Shayna Sarosiek
OBJECTIVE: To identify the prevalence of monoclonal gammopathy of undetermined significance (MGUS) in patients with transthyretin (ATTR) amyloidosis. PATIENTS AND METHODS: We performed a retrospective analysis of patients with biopsy-proven ATTRwt (wild-type transthyretin amyloid protein) and genopositive ATTR V122I (valine-to-isoleucine substitution at position 122 of the TTR gene) amyloidosis evaluated at the Amyloidosis Center at Boston University and Boston Medical Center between 1 January 2003 and 31 December 2016...
February 9, 2018: Amyloid: the International Journal of Experimental and Clinical Investigation
Ana M Gil, Daniela Duarte, Joana Pinto, António S Barros
This nuclear magnetic resonance metabolomics study compared the influence of two different Central Portugal exposomes, one of which comprising an important source of pollutants (the Estarreja Chemical Complex, ECC), on the urinary metabolic trajectory of a cohort of healthy pregnant women (total n=107). An exposome-independent description of pregnancy metabolism was found to comprise a set of 18 metabolites reflecting expected changes in branched-chain-amino acids catabolism, hormone and lipid metabolisms. In addition, a set of small changes in some metabolites were suggested to be exposome-dependent and characteristic of pregnant subjects from the Estarreja region...
February 9, 2018: Journal of Proteome Research
M Guevara-Cruz, J M Vargas-Morales, A L Méndez-García, A M López-Barradas, O Granados-Portillo, G Ordaz-Nava, A K Rocha-Viggiano, C A Gutierrez-Leyte, E Medina-Cerda, J L Rosado, J C Morales, N Torres, A R Tovar, L G Noriega
BACKGROUND AND AIMS: An increase in plasma branched-chain amino acids is associated with a higher risk of developing type 2 diabetes and cardiovascular diseases. However, little is known about the basal plasma amino acid concentrations in young adults. Our aim was to determine the plasma amino acid profiles of young adults and to evaluate how these profiles were modified by sex, body mass index (BMI) and insulin resistance (IR). METHODS AND RESULTS: We performed a transversal study with 608 Mexican young adults aged 19...
January 10, 2018: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
Felipe Campos de Almeida, Bruna Karina Banin Hirata, Carolina Batista Ariza, Roberta Losi Guembarovski, Karen Brajão de Oliveira, Karen Mayumi Suzuki, Alda Losi Guembarovski, Julie Massayo Maeda Oda, Glauco Akelinghton Freire Vitiello, Maria Angelica Ehara Watanabe
BACKGROUND: The HER2 (human epidermal growth factor receptor-2) Ile655Val (rs1136201) genetic polymorphism can alter the receptor structure and its auto-activation, which can modify the signal transduction and, consequently, the cell cycle regulation. For this reason, this polymorphism has been extensively investigated as a candidate marker for breast cancer (BC). In this context, the aim of this study was to evaluate the possible influence of HER2 Ile655Val in BC susceptibility and prognostic factors in a Brazilian population...
February 8, 2018: Journal of Clinical Laboratory Analysis
Paige M E Hawkins, Andrew M Giltrap, Gayathri Nagalingam, Warwick J Britton, Richard J Payne
The first total synthesis of the potent anti-mycobacterial cyclic depsipeptide natural product ecumicin is described. Synthesis was achieved via a solid-phase strategy, incorporating the synthetic non-proteinogenic amino acids N-methyl-4-methoxy-l-tryptophan and threo-β-hydroxy-l-phenylalanine into the growing linear peptide chain. The synthesis employed key on-resin esterification and dimethylation steps as well as a final macrolactamization between the unusual N-methyl-4-methoxy-l-tryptophan unit and a bulky N-methyl-l-valine residue...
February 7, 2018: Organic Letters
Jinhuan Qi, Xiaoxiao Zhang, Zhenlang Chen, Yunfan Shi, Qiuyun Liu
No abstract text is available yet for this article.
January 1, 2018: European Journal of Preventive Cardiology
Lei Zhu, Zhi Jun Zhao, Xiao Bin Ren, Qiang Li, Hua Ding, Zhou Sun, Qing Jun Kao, Li Hua Wang
The molecular pathogenesis of T-2 toxin-induced cartilage destruction has not been fully unraveled yet. The aim of this study was to detect changes in serum metabolites in a rat anomaly model with articular cartilage destruction. Thirty healthy male Wistar rats were fed a diet containing T-2 toxin (300 ng/kg chow) for 3 months. Histopathological changes in femorotibial cartilage were characterized in terms of chondrocyte degeneration/necrosis and superficial cartilage defect, and the endogenous metabolite profile of serum was determined by UPLC/Q-TOF MS...
January 2018: Biomedical and Environmental Sciences: BES
Hyang-Gi Ji, Yeong-Ran Lee, Min-Seuk Lee, Kyeong-Hwan Hwang, Clara Yongjoo Park, Eun-Hee Kim, Jun Seong Park, Young-Shick Hong
With increase of tea (Camellia sinensis) consumption, its chemical or metabolite compositions play a crucial role in the determination of tea quality. In general, metabolite compositions of fresh tea leaves including shoots depend on plucking seasons and tea cultivators. Therefore, choosing specific plucking time of tea leaves can provide use-specified tea products. Artificial control of tea growing, typically shade treatments, can lead to significant changes of the tea metabolite compositions of tea. However, metabolic characteristics of tea grown under various shade treatments conditions remain unclear...
February 6, 2018: Journal of Agricultural and Food Chemistry
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