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https://www.readbyqxmd.com/read/28932219/improving-acetic-acid-production-by-over-expressing-pqq-adh-in-acetobacter-pasteurianus
#1
Xuefeng Wu, Hongli Yao, Lili Cao, Zhi Zheng, Xiaoju Chen, Min Zhang, Zhaojun Wei, Jieshun Cheng, Shaotong Jiang, Lijun Pan, Xingjiang Li
Pyrroquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) is a key enzyme in the ethanol oxidase respiratory chain of acetic acid bacteria (AAB). To investigate the effect of PQQ-ADH on acetic acid production by Acetobacter pasteurianus JST-S, subunits I (adhA) and II (adhB) of PQQ-ADH were over-expressed, the fermentation parameters and the metabolic flux analysis were compared in the engineered strain and the original one. The acetic acid production was improved by the engineered strain (61.42 g L(-1)) while the residual ethanol content (4...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28883836/the-effect-of-pyrroloquinoline-quinone-on-the-expression-of-wisp1-in-traumatic-brain-injury
#2
Yongqi Ye, Pengju Zhang, Yuhang Qian, Baoxin Yin, Meijuan Yan
WISP1, as a member of the CCN4 protein family, has cell protective effects of promoting cell proliferation and inhibiting cell apoptosis. Although some studies have confirmed that WISP1 is concerned with colon cancer and lung cancer, there is little report about the influence of WISP1 in traumatic brain injury. Here, we found that the expression of WISP1 mRNA and protein decreased at 3 d and then increased at 5 d after traumatic brain injury (TBI). Meanwhile, immunofluorescence demonstrated that there was little colocation of WISP1 with GFAP, Iba1, and WISP1 colocalized with NeuN partly...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28709697/effects-of-p-limitation-and-molecules-from-peanut-root-exudates-on-pqqe-gene-expression-and-pqq-promoter-activity-in-the-phosphate-solubilizing-strain-serratia-sp-s119
#3
Liliana M Ludueña, Maria S Anzuay, Cynthia Magallanes-Noguera, Maria L Tonelli, Fernando J Ibañez, Jorge G Angelini, Adriana Fabra, Matthew McIntosh, Tania Taurian
The mineral phosphate-solubilizing phenotype in bacteria is attributed predominantly to secretion of gluconic acid produced by oxidation of glucose by the glucose dehydrogenase enzyme and its cofactor, pyrroloquinoline quinone. This study analyzes pqqE gene expression and pqq promoter activity in the native phosphate-solubilizing bacterium Serratia sp S119 growing under P-limitation, and in the presence of root exudates obtained from peanut plants, also growing under P-limitation. Results indicated that Serratia sp...
July 11, 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28676501/lifespan-extension-by-peroxidase-dual-oxidase-mediated-ros-signaling-through-pyrroloquinoline-quinone-in-c-elegans
#4
Hiroyuki Sasakura, Hiroki Moribe, Masahiko Nakano, Kazuto Ikemoto, Kosei Takeuchi, Ikue Mori
Reactive oxygen species (ROS), originally characterized based on their harmful effects on cells or organisms, are now recognized as important signal molecules regulating various biological processes. In particular, low levels of ROS released from mitochondria extend lifespan. Here, we identified a novel mechanism of generating appropriate levels of ROS at the plasma membrane through a peroxidase/dual oxidase system, which could extend lifespan in Caenorhabditis elegans (C. elegans). A redox co-factor, pyrroloquinoline quinone (PQQ), activates dual oxidase (DUOX)/BLI-3 to produce the ROS H2O2 at the plasma membrane, which is subsequently degraded by peroxidase/MLT-7, eventually ensuring the adequate levels of ROS...
July 4, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28672774/combinatorial-therapy-of-exercise-preconditioning-and-nanocurcumin-formulation-supplementation-improves-cardiac-adaptation-under-hypobaric-hypoxia
#5
Sarita Nehra, Varun Bhardwaj, Anju Bansal, Deepika Saraswat
BACKGROUND: Chronic hypobaric hypoxia (cHH) mediated cardiac insufficiencies are associated with pathological damage. Sustained redox stress and work load are major causative agents of cardiac insufficiencies under cHH. Despite the advancements made in pharmacological (anti-oxidants, vasodilators) and non-pharmacological therapeutics (acclimatization strategies and schedules), only partial success has been achieved in improving cardiac acclimatization to cHH. This necessitates the need for potent combinatorial therapies to improve cardiac acclimatization at high altitudes...
September 26, 2017: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/28655819/functional-role-of-lanthanides-in-enzymatic-activity-and-transcriptional-regulation-of-pyrroloquinoline-quinone-dependent-alcohol-dehydrogenases-in-pseudomonas-putida-kt2440
#6
Matthias Wehrmann, Patrick Billard, Audrey Martin-Meriadec, Asfaw Zegeye, Janosch Klebensberger
The oxidation of alcohols and aldehydes is crucial for detoxification and efficient catabolism of various volatile organic compounds (VOCs). Thus, many Gram-negative bacteria have evolved periplasmic oxidation systems based on pyrroloquinoline quinone-dependent alcohol dehydrogenases (PQQ-ADHs) that are often functionally redundant. Here we report the first description and characterization of a lanthanide-dependent PQQ-ADH (PedH) in a nonmethylotrophic bacterium based on the use of purified enzymes from the soil-dwelling model organism Pseudomonas putida KT2440...
June 27, 2017: MBio
https://www.readbyqxmd.com/read/28578007/sensitivity-of-hipsc-derived-neural-stem-cells-nsc-to-pyrroloquinoline-quinone-depends-on-their-developmental-stage
#7
J Augustyniak, J Lenart, M Zychowicz, G Lipka, P Gaj, M Kolanowska, P P Stepien, L Buzanska
Pyrroloquinoline quinone (PQQ) is a factor influencing on the mitochondrial biogenesis. In this study the PQQ effect on viability, total cell number, antioxidant capacity, mitochondrial biogenesis and differentiation potential was investigated in human induced Pluripotent Stem Cells (iPSC) - derived: neural stem cells (NSC), early neural progenitors (eNP) and neural progenitors (NP). Here we demonstrated that sensitivity to PQQ is dependent upon its dose and neural stage of development. Induction of the mitochondrial biogenesis by PQQ at three stages of neural differentiation was evaluated at mtDNA, mRNA and protein level...
May 31, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28572558/synthesis-and-crystal-structure-of-pyrroloquinoline-quinol-pqqh2-and-pyrroloquinoline-quinone-pqq
#8
Kazuto Ikemoto, Shigeki Mori, Kazuo Mukai
Pyrroloquinoline quinone (PQQ) is a water-soluble quinone compound first identified as a cofactor of alcohol- and glucose-dehydrogenases (ADH and GDH) in bacteria. For example, in the process of ADH reaction, alcohol is oxidized to the corresponding aldehyde, and inversely PQQ is reduced to pyrroloquinoline quinol (PQQH2). PQQ and PQQH2 molecules play an important role as a cofactor in ADH and GDH reactions. However, crystal structure analysis has not been performed for PQQ and PQQH2. In the present study, the synthesis of PQQH2 powder crystals was performed under air, by utilizing vitamin C as a reducing agent...
June 1, 2017: Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials
https://www.readbyqxmd.com/read/28500115/erroneously-elevated-glucose-values-due-to-maltose-interference-in-mutant-glucose-dehydrogenase-pyrroloquinolinequinone-mutant-gdh-pqq-based-glucometer
#9
Partha Pratim Chakraborty, Shinjan Patra, Rana Bhattacharjee, Subhankar Chowdhury
Currently available glucose test strip enzymes include glucose oxidase (GOD) and glucose dehydrogenase (GDH). In GDH-based glucometers, glucose oxidation can be catalysed by different cofactors: nicotinamide adenine dinucleotide (GDH-NAD), flavin adenine dinucleotide (GDH-FAD), pyrroloquinolinequinone (GDH-PQQ) and mutant GDH-PQQ. GOD-based and GDH-NAD-based glucometers are substrate-specific and do not react with sugars other than glucose. GDH-FAD reacts with xylose only in addition to glucose. GDH-PQQ is not glucose-specific; in addition to glucose, it reacts with different other sugars and produces falsely high values of capillary glucose in the presence of such substances...
May 12, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28484812/metabolic-engineering-of-gluconobacter-oxydans-621h-for-increased-biomass-yield
#10
Ines Kiefler, Stephanie Bringer, Michael Bott
The obligatory aerobic acetic acid bacterium Gluconobacter oxydans incompletely oxidizes carbon sources regio- and stereoselectively in the periplasm and therefore is used industrially for oxidative biotransformations, e. g., in vitamin C production. However, it has a very low biomass yield as the oxidized products largely remain in the medium and cannot be used for anabolism. Cytoplasmic carbon metabolism occurs via the pentose phosphate pathway and the Entner-Doudoroff pathway, whereas glycolysis and the tricarboxylic acid cycle are incomplete...
July 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28481092/nuclear-magnetic-resonance-structure-and-binding-studies-of-pqqd-a-chaperone-required-in-the-biosynthesis-of-the-bacterial-dehydrogenase-cofactor-pyrroloquinoline-quinone
#11
Robert L Evans, John A Latham, Youlin Xia, Judith P Klinman, Carrie M Wilmot
Biosynthesis of the ribosomally synthesized and post-translationally modified peptide (RiPP), pyrroloquinoline quinone (PQQ), is initiated when the precursor peptide, PqqA, is recognized and bound by the RiPP precursor peptide recognition element (RRE), PqqD, for presentation to the first enzyme in the pathway, PqqE. Unlike other RiPP-producing, postribosomal peptide synthesis (PRPS) pathways in which the RRE is a component domain of the first enzyme, PqqD is predominantly a separate scaffolding protein that forms a ternary complex with the precursor peptide and first tailoring enzyme...
May 30, 2017: Biochemistry
https://www.readbyqxmd.com/read/28469179/highly-selective-and-sensitive-self-powered-glucose-sensor-based-on-capacitor-circuit
#12
Gymama Slaughter, Tanmay Kulkarni
Enzymatic glucose biosensors are being developed to incorporate nanoscale materials with the biological recognition elements to assist in the rapid and sensitive detection of glucose. Here we present a highly sensitive and selective glucose sensor based on capacitor circuit that is capable of selectively sensing glucose while simultaneously powering a small microelectronic device. Multi-walled carbon nanotubes (MWCNTs) is chemically modified with pyrroloquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BOD) at anode and cathode, respectively, in the biofuel cell arrangement...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28454714/growth-promotion-of-peanut-arachis-hypogaea-l-and-maize-zea-mays-l-plants-by-single-and-mixed-cultures-of-efficient-phosphate-solubilizing-bacteria-that-are-tolerant-to-abiotic-stress-and-pesticides
#13
María Soledad Anzuay, María Gabriela Ruiz Ciancio, Liliana Mercedes Ludueña, Jorge Guillermo Angelini, Germán Barros, Nicolás Pastor, Tania Taurian
The aims of this study were, to analyze in vitro phosphate solubilization activity of six native peanut bacteria and to determine the effect of single and mixed inoculation of these bacteria on peanut and maize plants. Ability to produce organic acids and cofactor PQQ, to solubilize FePO4 and AlPO4 and phosphatase activity were analyzed. Also, the ability to solubilize phosphate under abiotic stress and in the presence of pesticides of the selected bacteria was determined. The effect of single and mixed bacterial inocula was analyzed on seed germination, maize plant growth and in a crop rotation plant assay with peanut and maize...
June 2017: Microbiological Research
https://www.readbyqxmd.com/read/28448745/medium-optimization-for-pyrroloquinoline-quinone-pqq-production-by-methylobacillus-sp-zju323-using-response-surface-methodology-and-artificial-neural-network-genetic-algorithm
#14
Peilian Wei, Zhenjun Si, Yao Lu, Qingfei Yu, Lei Huang, Zhinan Xu
Methylobacillus sp. zju323 was adopted to improve the biosynthesis of pyrroloquinoline quinone (PQQ) by systematic optimization of the fermentation medium. The Plackett-Burman design was implemented to screen for the key medium components for the PQQ production. CoCl2 · 6H2O, ρ-amino benzoic acid, and MgSO4 · 7H2O were found capable of enhancing the PQQ production most significantly. A five-level three-factor central composite design was used to investigate the direct and interactive effects of these variables...
August 9, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28413833/beneficial-effects-of-a-pyrroloquinolinequinone-containing-dietary-formulation-on-motor-deficiency-cognitive-decline-and-mitochondrial-dysfunction-in-a-mouse-model-of-alzheimer-s-disease
#15
Darrell Sawmiller, Song Li, Takashi Mori, Ahsan Habib, David Rongo, Vedad Delic, Patrick C Bradshaw, R Douglas Shytle, Cyndy Sanberg, Paula Bickford, Jun Tan
Alzheimer's disease (AD), a progressive neurodegenerative disorder, is linked to oxidative stress, altered amyloid precursor protein (APP) proteolysis, tau hyperphosphorylation and the accumulation of amyloid-β (Aβ) plaques and neurofibrillary tangles (NFT). A growing body of evidence suggests that mitochondrial dysfunction can be a key promoter of all of these pathologies and predicts that restoration of mitochondrial function might be a potential therapeutic strategy for AD. Therefore, in the present study, we tested the beneficial effect of a nutraceutical formulation Nutrastem II (Nutra II), containing NT020 (a mitochondrial restorative and antioxidant proprietary formulation) and pyrroloquinolinequinone (PQQ, a stimulator of mitochondria biogenesis) in 5XFAD transgenic mice...
April 2017: Heliyon
https://www.readbyqxmd.com/read/28407913/pyrroloquinoline-quinone-from-gluconobacter-oxydans-fermentation-broth-enhances-superoxide-anion-scavenging-capacity-of-cu-zn-sod
#16
Ke Ma, Jun-Zhu Cui, Jian-Bin Ye, Xian-Mei Hu, Ge-Li Ma, Xue-Peng Yang
A bioassay-guided fractionation of extract from Gluconobacter oxydans fermentation broth afforded Compound 1, which was identified as pyrroloquinoline quinone (PQQ) by spectroscopic methods. PQQ has been shown to enhance the superoxide anion-scavenging capacity significantly for Cu/Zn-SOD. To illustrate the mechanism, the interaction between PQQ and Cu/Zn-SOD was investigated. The multiple binding sites involving hydrogen bonds and van der Waals force between PQQ and Cu/Zn-SOD were revealed by isothermal titration calorimetry...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28386349/pyrroloquinoline-quinone-prevents-testosterone-deficiency-induced-osteoporosis-by-stimulating-osteoblastic-bone-formation-and-inhibiting-osteoclastic-bone-resorption
#17
Xuan Wu, Jie Li, Hengwei Zhang, Hui Wang, Guoyong Yin, Dengshun Miao
Accumulating evidences suggest that oxidative stress caused and deteriorated the aging related osteoporosis and pyrroloquinoline quinone (PQQ) is a powerful antioxidant. However, it is unclear whether PQQ can prevent testosterone deficiency-induced osteoporosis. In this study, the orchidectomized (ORX) mice were supplemented in diet with/without PQQ for 48 weeks, and compared with each other and with sham mice. Results showed that bone mineral density, trabecular bone volume, collagen deposition and osteoblast number were decreased significantly in ORX mice compared with shame mice, whereas PQQ supplementation largely prevented these alterations...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28374050/enhanced-fed-batch-production-of-pyrroloquinoline-quinine-in-methylobacillus-sp-cctcc-m2016079-with-a-two-stage-ph-control-strategy
#18
Zhenjun Si, David Machaku, Peilian Wei, Lei Huang, Jin Cai, Zhinan Xu
The effects of pH control strategy and fermentative operation modes on the biosynthesis of pyrroloquinoline quinine (PQQ) were investigated systematically with Methylobacillus sp. CCTCC M2016079 in the present work. Firstly, the shake-flask cultivations and benchtop fermentations at various pH values ranging from 5.3 to 7.8 were studied. Following a kinetic analysis of specific cell growth rate (μ x ) and specific PQQ formation rate (μ p ), the discrepancy in optimal pH values between cell growth and PQQ biosynthesis was observed, which stimulated us to develop a novel two-stage pH control strategy...
April 3, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28344084/a-rapid-procedure-for-the-in-situ-assay-of-periplasmic-pqq-dependent-methanol-dehydrogenase-in-intact-single-bacterial-colonies
#19
Venkata Ramana Vemuluri, Shreya Shaw, Caroline Autenrieth, Robin Ghosh
Mechanistic details of methanol oxidation catalyzed by the periplasmically-located pyrroloquinoline quinone-dependent methanol dehydrogenase of methylotrophs can be elucidated using site-directed mutants. Here, we present an in situ colony assay of methanol dehydrogenase, which allows robotic screening of large populations of intact small colonies, and regrowth of colonies for subsequent analysis.
June 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28283693/genetically-engineered-escherichia-coli-nissle-1917-synbiotic-counters-fructose-induced-metabolic-syndrome-and-iron-deficiency
#20
Archana Somabhai Chaudhari, Ruma Raghuvanshi, G Naresh Kumar
Consumption of fructose leads to metabolic syndrome, but it is also known to increase iron absorption. Present study investigates the effect of genetically modified Escherichia coli Nissle 1917 (EcN) synbiotic along with fructose on non-heme iron absorption. Charles foster rats weighing 150-200 g were fed with iron-deficient diet for 2 months. Probiotic treatment of EcN (pqq) and EcN (pqq-glf-mtlK) was given once per week, 10(9) cells after 2 months with fructose in drinking water. Iron levels, blood, and liver parameters for oxidative stress, hyperglycemia, and dyslipidemia were estimated...
March 10, 2017: Applied Microbiology and Biotechnology
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