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https://www.readbyqxmd.com/read/28155960/connecting-quantum-dots-with-enzymes-mediator-based-approaches-for-the-light-directed-read-out-of-glucose-and-fructose-oxidation
#1
M Riedel, N Sabir, F W Scheller, W J Parak, F Lisdat
The combination of the biocatalytic features of enzymes with the unique physical properties of nanoparticles in a biohybrid system provides a promising approach for the development of advanced bioelectrocatalytic devices. This study describes the construction of photoelectrochemical signal chains based on CdSe/ZnS quantum dot (QD) modified gold electrodes as light switchable elements, and low molecular weight redox molecules for the combination with different biocatalysts. Photoelectrochemical and photoluminescence experiments verify that electron transfer can be achieved between the redox molecules hexacyanoferrate and ferrocene, and the QDs under illumination...
February 3, 2017: Nanoscale
https://www.readbyqxmd.com/read/28124619/the-effect-of-pyrroloquinoline-quinone-on-apoptosis-and-autophagy-in-traumatic-brain-injury
#2
Pengju Zhang, Yongqi Ye, Yuhang Qian, Baoxin Yin, Jianmei Zhao, Shunxin Zhu, Li Zhang, Meijuan Yan
BACKGROUND: Pyrroloquinoline quinoe is an anionic, water-soluble compound with antioxidant characteristic. The role of pyrroloquinoline quinoe in pharmacology and nutrition has attracted wide attention of researchers. Although a few experiments have confirmed that pyrroloquinoline quinoe plays an obvious effective role in neuroprotection. There are little reports about the effect of pyrroloquinoline quinoe in traumatic brain injury. Traumatic brain injury is one of the leading causes of adult disability and death in the world...
January 24, 2017: CNS & Neurological Disorders Drug Targets
https://www.readbyqxmd.com/read/28115068/the-role-of-oxidative-stress-in-dna-damage-in-pancreatic-%C3%AE-cells-induced-by-di-2-ethylhexyl-phthalate
#3
Yan She, Liping Jiang, Liangliang Zheng, Heming Zuo, Min Chen, Xiance Sun, Qiujuan Li, Chengyan Geng, Guang Yang, Lijie Jiang, Xiaofang Liu
Di(2-ethyhexyl) phthalate (DEHP) is commonly used as a plasticizer, which loosely binds to plastic materials and easily leaches out of these products and enters into the environment. Exposure to DEHP can impair pancreatic beta cells (INS-1 cells)function, which is associated with Insulin Resistance (IR) and type 2 diabetes. However, the mechanism of how DEHP leads to Insulin Resistance is unknown. Our results showed that the cell viability of INS-1 cells exposed to DEHP (0-1600 μM) were decreased in a concentration-dependent manner...
January 20, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28105098/radioprotective-effects-of-pyrroloquinoline-quinone-on-parotid-glands-in-c57bl-6j-mice
#4
Yuanqing Huang, Ning Chen, Dengshun Miao
The aim of the present study was to investigate whether pyrroloquinoline quinine (PQQ) serve a radioprotective role in parotid gland damage induced by total body irradiation (TBI) in C57BL/6J mice. A total of 15 female 8-week-old C57BL/6J mice were randomly assigned into three treatment groups: i) Untreated control (no irradiation); ii) 4 gray (Gy) X-ray irradiation; iii) 4 Gy X-ray irradiation with additional dietary PQQ (4 mg PQQ/kg in normal diet). Each group included five mice. After 4 weeks, all animals were collected for evaluating the phenotype, body weight, pathological and biochemical parameters...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28063921/tricalcium-phosphate-solubilization-and-nitrogen-fixation-by-newly-isolated-aneurinibacillus-aneurinilyticus-ckmv1-from-rhizosphere-of-valeriana-jatamansi-and-its-growth-promotional-effect
#5
Anjali Chauhan, Shiwani Guleria, Praveen P Balgir, Abhishek Walia, Rishi Mahajan, Preeti Mehta, Chand Karan Shirkot
Aneurinibacillus aneurinilyticus strain CKMV1 was isolated from rhizosphere of Valeriana jatamansi and possessed multiple plant growth promoting traits like production of phosphate solubilization (260mg/L), nitrogen fixation (202.91nmolethylenemL(-1)h(-1)), indole-3-acetic acid (IAA) (8.1μg/mL), siderophores (61.60%), HCN (hydrogen cyanide) production and antifungal activity. We investigated the ability of isolate CKMV1 to solubilize insoluble P via mechanism of organic acid production. High-performance liquid chromatography (HPLC) study showed that isolate CKMV1 produced mainly gluconic (1...
December 24, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28007783/early-pqq-supplementation-has-persistent-long-term-protective-effects-on-developmental-programming-of-hepatic-lipotoxicity-and-inflammation-in-obese-mice
#6
Karen R Jonscher, Michael S Stewart, Alba Alfonso-Garcia, Brian C DeFelice, Xiaoxin X Wang, Yuhuan Luo, Moshe Levi, Margaret J R Heerwagen, Rachel C Janssen, Becky A de la Houssaye, Ellen Wiitala, Garrett Florey, Raleigh L Jonscher, Eric O Potma, Oliver Fiehn, Jacob E Friedman
Nonalcoholic fatty liver disease (NAFLD) is widespread in adults and children. Early exposure to maternal obesity or Western-style diet (WD) increases steatosis and oxidative stress in fetal liver and is associated with lifetime disease risk in the offspring. Pyrroloquinoline quinone (PQQ) is a natural antioxidant found in soil, enriched in human breast milk, and essential for development in mammals. We investigated whether a supplemental dose of PQQ, provided prenatally in a mouse model of diet-induced obesity during pregnancy, could protect obese offspring from progression of NAFLD...
December 22, 2016: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27951709/amphiphilic-polymer-mediators-promoting-electron-transfer-on-bioanodes-with-pqq-dependent-glucose-dehydrogenase
#7
Yasuo Nakashima, Norihiro Mizoshita, Hiromitsu Tanaka, Yuichiro Nakaoki
Redox-active phenazinium salts bonded to amphiphilic polymer backbones are demonstrated to function as high-performance electron-transfer mediators in enzymatic bioanodes applicable to biofuel cells. The redox-active moieties could be easily tethered to the electrodes by physical adsorption of the hydrophobic regions of the polymer backbones onto the electrode surface. On the other hand, long hydrophilic chains were essential to ensure high mobility of the redox-active moieties in aqueous solutions and to enhance their electron-transfer properties...
December 13, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27876710/enhancement-of-1-3-dihydroxyacetone-production-from-gluconobacter-oxydans-by-combined-mutagenesis
#8
Xi Lin, Sha Liu, Guangrong Xie, Jing Chen, Penghua Li, Jianhua Chen
Wild strain L-6 was subjected to combined mutagenesis, including UV irradiation, atmospheric and room temperature plasma, and ion beam implantation, to increase the yield of 1,3-dihydroxyacetone (DHA). With application of a high-throughput screening method, mutant Gluconobacter oxydans I-2-239 with a DHA productivity of 103.5 g/l in flask-shake fermentation was finally obtained with the starting glycerol concentration of 120 g/l, which was 115.7% higher than the wild strain. The cultivation time also decreased from 54 h to 36 h...
November 28, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27849146/membrane-bound-glycerol-dehydrogenase-catalyzes-oxidation-of-d-pentonates-to-4-keto-d-pentonates-d-fructose-to-5-keto-d-fructose-and-d-psicose-to-5-keto-d-psicose
#9
Yoshitaka Ano, Roque A Hours, Yoshihiko Akakabe, Naoya Kataoka, Toshiharu Yakushi, Kazunobu Matsushita, Osao Adachi
A novel oxidation of D-pentonates to 4-keto-D-pentonates was analyzed with Gluconobacter thailandicus NBRC 3258. D-Pentonate 4-dehydrogenase activity in the membrane fraction was readily inactivated by EDTA and it was reactivated by the addition of PQQ and Ca(2+). D-Pentonate 4-dehydrogenase was purified to two different subunits, 80 and 14 kDa. The absorption spectrum of the purified enzyme showed no typical absorbance over the visible regions. The enzyme oxidized D-pentonates to 4-keto-D-pentonates at the optimum pH of 4...
February 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/27836608/an-improved-glycerol-biosensor-with-an-au-fes-nad-glycerol-dehydrogenase-anode
#10
Aishwarya Mahadevan, Sandun Fernando
An improved glycerol biosensor was developed via direct attachment of NAD(+)-glycerol dehydrogenase coenzyme-apoenzyme complex onto supporting gold electrodes, using novel inorganic iron (II) sulfide (FeS)-based single molecular wires. Sensing performance factors, i.e., sensitivity, a detection limit and response time of the FeS and conventional pyrroloquinoline quinone (PQQ)-based biosensor were evaluated by dynamic constant potential amperometry at 1.3V under non-buffered conditions. For glycerol concentrations ranging from 1 to 25mM, a 77% increase in sensitivity and a 53% decrease in detection limit were observed for the FeS-based biosensor when compared to the conventional PQQ-based counterpart...
October 31, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27805779/a-nernstian-biosupercapacitor
#11
Dmitry Pankratov, Felipe Conzuelo, Piyanut Pinyou, Sabine Alsaoub, Wolfgang Schuhmann, Sergey Shleev
We propose the very first "Nernstian biosupercapacitor", a biodevice based on only one redox polymer: poly(vinyl imidazole-co-allylamine)[Os(bpy)2 Cl], and two biocatalysts. At the bioanode PQQ-dependent glucose dehydrogenase reduces the Os(3+) moieties at the polymer to Os(2+) shifting the Nernst potential of the Os(3+) /Os(2+) redox couple to negative values. Concomitantly, at the biocathode the reduction of O2 by means of bilirubin oxidase embedded in the same redox polymer leads to the oxidation of Os(2+) to Os(3+) shifting the Nernst potential to higher values...
December 5, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27760187/genetically-engineered-escherichia-coli-nissle-1917-synbiotics-reduce-metabolic-effects-induced-by-chronic-consumption-of-dietary-fructose
#12
Chaudhari Archana Somabhai, Ruma Raghuvanshi, G Nareshkumar
AIMS: To assess protective efficacy of genetically modified Escherichia coli Nissle 1917 (EcN) on metabolic effects induced by chronic consumption of dietary fructose. MATERIALS AND METHODS: EcN was genetically modified with fructose dehydrogenase (fdh) gene for conversion of fructose to 5-keto-D-fructose and mannitol-2-dehydrogenase (mtlK) gene for conversion to mannitol, a prebiotic. Charles foster rats weighing 150-200 g were fed with 20% fructose in drinking water for two months...
2016: PloS One
https://www.readbyqxmd.com/read/27638737/-1-h-13-c-and-15-n-resonance-assignments-and-secondary-structure-information-for-methylobacterium-extorquens-pqqd-and-the-complex-of-pqqd-with-pqqa
#13
Robert L Evans, John A Latham, Judith P Klinman, Carrie M Wilmot, Youlin Xia
The ribosomally synthesized and post-translationally modified peptide (RiPP), pyrroloquinoline quinone (PQQ), is a dehydrogenase cofactor synthesized by, but not exclusively used by, certain prokaryotes. RiPPs represent a rapidly expanding and diverse class of natural products-many of which have therapeutic potential-and the biosynthetic pathways for these are gaining attention. Five gene products from the pqq operon (PqqA, PqqB, PqqC, PqqD, and PqqE) are essential for PQQ biosynthesis. The substrate is the peptide PqqA, which is presented to the radical SAM enzyme PqqE by the small protein PqqD...
October 2016: Biomolecular NMR Assignments
https://www.readbyqxmd.com/read/27625643/determination-of-dehydrogenase-activities-involved-in-d-glucose-oxidation-in-gluconobacter-and-acetobacter-strains
#14
Florencia Sainz, María Jesús Torija, Minenosuke Matsutani, Naoya Kataoka, Toshiharu Yakushi, Kazunobu Matsushita, Albert Mas
Acetic acid bacteria (AAB) are known for rapid and incomplete oxidation of an extensively variety of alcohols and carbohydrates, resulting in the accumulation of organic acids as the final products. These oxidative fermentations in AAB are catalyzed by PQQ- or FAD- dependent membrane-bound dehydrogenases. In the present study, the enzyme activity of the membrane-bound dehydrogenases [membrane-bound PQQ-glucose dehydrogenase (mGDH), D-gluconate dehydrogenase (GADH) and membrane-bound glycerol dehydrogenase (GLDH)] involved in the oxidation of D-glucose and D-gluconic acid (GA) was determined in six strains of three different species of AAB (three natural and three type strains)...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27573017/pyrroloquinoline-quinone-ethanol-dehydrogenase-in-methylobacterium-extorquens-am1-extends-lanthanide-dependent-metabolism-to-multicarbon-substrates
#15
Nathan M Good, Huong N Vu, Carly J Suriano, Gabriel A Subuyuj, Elizabeth Skovran, N Cecilia Martinez-Gomez
: Lanthanides are utilized by microbial methanol dehydrogenases, and it has been proposed that lanthanides may be important for other type I alcohol dehydrogenases. A triple mutant strain (mxaF xoxF1 xoxF2; named MDH-3), deficient in the three known methanol dehydrogenases of the model methylotroph Methylobacterium extorquens AM1, is able to grow poorly with methanol if exogenous lanthanides are added to the growth medium. When the gene encoding a putative quinoprotein ethanol dehydrogenase, exaF, was mutated in the MDH-3 background, the quadruple mutant strain could no longer grow on methanol in minimal medium with added lanthanum (La(3+))...
November 15, 2016: Journal of Bacteriology
https://www.readbyqxmd.com/read/27565673/comparative-analysis-of-l-sorbose-dehydrogenase-by-docking-strategy-for-2-keto-l-gulonic-acid-production-in-ketogulonicigenium-vulgare-and-bacillus-endophyticus-consortium
#16
Si Chen, Nan Jia, Ming-Zhu Ding, Ying-Jin Yuan
Improving the yield of 2-keto-L-gulonic acid (2-KGA), the direct precursor of vitamin C, draws more and more attention in industrial production. In this study, we try to increase the 2-KGA productivity by computer-aided selection of genes encoding L-sorbose dehydrogenases (SDH) of Ketogulonicigenium vulgare. First, six SDHs were modeled by docking strategy to predict the binding mode with co-factor PQQ. The binding energy between SSDA1-H/SSDA1-L and PQQ was the highest, followed by SSDA3/SSDA2. The binding energy between SSDA1-P/SSDB and PQQ was the lowest...
November 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27526146/effects-of-antioxidant-supplements-biopqq%C3%A2-on-cerebral-blood-flow-and-oxygen-metabolism-in-the-prefrontal-cortex
#17
Masahiko Nakano, Yuta Murayama, Lizhen Hu, Kazuto Ikemoto, Tatsuo Uetake, Kaoru Sakatani
Pyrroloquinoline quinone (PQQ) is a quinone compound originally identified in methanol-utilizing bacteria and is a cofactor for redox enzymes. At the Meeting of the International Society on Oxygen Transport to Tissue (ISOTT) 2014, we reported that PQQ disodium salt (BioPQQ™) improved cognitive function in humans, as assessed by the Stroop test. However, the physiological mechanism of PQQ remains unclear. In the present study, we measured regional cerebral blood flow (rCBF) and oxygen metabolism in prefrontal cortex (PFC), before and after administration of PQQ, using time-resolved near-infrared spectroscopy (tNIRS)...
2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27491947/the-cooperativity-effect-in-the-reaction-of-soluble-quinoprotein-pqq-containing-glucose-dehydrogenase-is-not-due-to-subunit-interaction-but-to-substrate-assisted-catalysis
#18
Johannis A Duine, Marc J F Strampraad, Wilfred R Hagen, Simon de Vries
: Soluble quinoprotein (PQQ-containing) glucose dehydrogenase (sGDH, EC 1.1.99.35) catalyzes the oxidation of β-d-glucose to d-glucono-δ-lactone. Although sGDH has many analytical applications, the relationship between activity and substrate concentration is not well established. Previous steady-state kinetic studies revealed a negative cooperativity effect which has recently been ascribed to subunit interaction. To investigate this conclusion, stopped-flow kinetic experiments were carried out on the reaction in which oxidized enzyme (Eox ) was reduced with substrates to Ered ...
October 2016: FEBS Journal
https://www.readbyqxmd.com/read/27464830/novel-and-efficient-screening-of-pqq-high-yielding-strains-and-subsequent-cultivation-optimization
#19
Zhenjun Si, Jianzhong Zhu, Wenguang Wang, Lei Huang, Peilian Wei, Jin Cai, Zhinan Xu
A novel and efficient screening method for pyrroloquinoline quinone (PQQ) high-yielding methylotrophic strains was developed by using glucose dehydrogenase apoenzyme (GDHA) which depended on PQQ as the cofactor. Using this high-throughput method, PQQ high-yielding strains were rapidly screened out from thousands of methylotrophic colonies at a time. The comprehensive phylogenetic analysis revealed that the highest PQQ-producing strain zju323 (CCTCC M 2016079) could be assigned to a novel species in the genus Methylobacillus of the Betaproteobacteria...
December 2016: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27463000/engineered-pqq-glucose-dehydrogenase-as-a-universal-biosensor-platform
#20
Zhong Guo, Lindy Murphy, Viktor Stein, Wayne A Johnston, Siro Alcala-Perez, Kirill Alexandrov
Biosensors with direct electron output hold promise for nearly seamless integration with portable electronic devices. However, so far, they have been based on naturally occurring enzymes that significantly limit the spectrum of detectable analytes. Here, we present a novel biosensor architecture based on analyte-driven intermolecular recombination and activity reconstitution of a re-engineered component of glucometers: PQQ-glucose dehydrogenase. We demonstrate that this sensor architecture can be rapidly adopted for the detection of immunosuppressant drugs, α-amylase protein, or protease activity of thrombin and Factor Xa...
August 17, 2016: Journal of the American Chemical Society
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