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Haoxin Lv, Akio Tani
Oharaeibacter diazotrophicus strain SM30T , isolated from rice rhizosphere, is an aerobic, facultative lanthanide (Ln3+ )-utilizing methylotroph and diazotroph that belongs to the Methylocystaceae family. In this research, the complete genome sequence of strain SM30T was determined, and its methylotrophy modules were characterized. The genome consists of one chromosome and two plasmids, comprising a total of 5,004,097 bp, and the GC content was 71.6 mol%. A total of 4497 CDSs, 67 tRNA, and 9 rRNA were encoded...
June 15, 2018: Journal of Bioscience and Bioengineering
Hongyue Li, Weizhu Zeng, Jingwen Zhou
Pyrroloquinoline quinone (PQQ) is a bacterial dehydrogenase coenzyme. PQQ can promote body growth and regulate the function of free radical level of the body. It could be applied in food, medicine and other fields. Due to the extremely high cost of chemical synthesis, the production of PQQ by microbial fermentation attracted more and more attention. At present, the production titer of PQQ by fermentation method is too low to achieve industrial application. Due to the lack of a thorough understanding of the PQQ biosynthesis and its regulation mechanisms, and the lack of necessary genetic engineering modification methods for wild type strains, metabolic engineering of microorganisms to enhance PQQ production still lacks essential requirements...
May 25, 2018: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
Xing-Qin Zhou, Zhi-Wen Yao, Ying Peng, Shi-Shi Mao, Dong Xu, Xiao-Feng Qin, Rong-Jun Zhang
Oxidative stress is known to be associated with various age-related diseases. D-galactose (D-gal) has been considered a senescent model which induces oxidative stress response resulting in memory dysfunction. Pyrroloquinoline quinone (PQQ) is a redox cofactor which is found in various foods. In our previous study, we found that PQQ may be converted into a derivative by binding with amino acid, which is beneficial to several pathological processes. In this study, we found a beneficial glutamate mixture which may diminish neurotoxicity by oxidative stress in D-gal induced mouse...
June 11, 2018: Scientific Reports
Yusuke Yamauchi, Yosi Nindita, Keisuke Hara, Asako Umeshiro, Yu Yabuuchi, Toshihiro Suzuki, Haruyasu Kinashi, Kenji Arakawa
Reinvestigation of the metabolite profile in a disruptant of the quinoprotein dehydrogenase (orf23) gene revealed that the Orf23 protein catalyzes dehydrogenation of the C23-C25 lactate moiety to pyruvate during lankacidin biosynthesis in Streptomyces rochei 7434AN4. The dehydrogenase activity was expressed and detected in a soluble fraction of the Streptomyceslividans recombinant harboring orf23. The Orf23 protein preferentially converts lankacidinol to lankacidin C in the presence of pyrroloquinoline quinone (PQQ)...
June 2, 2018: Journal of Bioscience and Bioengineering
Paul Hwang, Darryn S Willoughby
There is clear evidence that endurance exercise training elicits intramuscular adaptations that can lead to elevations in mitochondrial biogenesis, oxidative capacity, mitochondrial density, and mitochondrial function. Mitochondrial biogenesis is regulated by the activation of the transcriptional coactivator peroxisome proliferator-activated receptor gamma coactivator 1-alpha. This master regulator of mitochondrial biogenesis activates nuclear respiratory factors (NRF-1, NRF-2) and mitochondrial transcription factor A, which enables the expansion of mitochondrial size and transcription of mitochondrial DNA...
May 1, 2018: Journal of the American College of Nutrition
Fato Tano Patrice, Kaipei Qiu, Li-Jun Zhao, Essy Kouadio Fodjo, Da-Wei Li, Yi-Tao Long
Inspired by the addition-elimination catalytic mechanism of natural pyrroloquinoline quinone (PQQ) containing proteins, PQQ-modified hybrid nanomaterials have been increasingly developed recently as biomimetic heterogeneous electrocatalysts. However, up until now, no existing electrochemical approach was able to assess the intrinsic catalytic activity of PQQ sites, impeding the design of efficient PQQ-based electrocatalysts. Herein, in this work, we introduced a new method to calculate the turnover frequency (TOF) of any individual PQQ functional group for electrocatalytic oxidation of tris(2-carboxyethyl)phosphine (TCEP), through the study of single PQQ-decorated carbon nanotube (CNT) collisions at a carbon fiber ultramicroelectrode by chronoamperometry...
May 15, 2018: Analytical Chemistry
Giovanni Fusco, Gero Göbel, Robertino Zanoni, Maria Paola Bracciale, Gabriele Favero, Franco Mazzei, Fred Lisdat
In this study, polythiophene copolymers have been used as modifier for electrode surfaces in order to allow the immobilization of active pyrroloquinoline quinone dependent glucose dehydrogenase (PQQ-GDH) and to simultaneously improve the direct electrical connection of the enzyme with the electrode. Polymer films are electrosynthesized in aqueous solution without the need of surfactants onto carbon nanotubes modified gold electrodes from mixtures of 3-thiopheneacetic acid (ThCH2 CO2 H) and 3-methoxythiophene (ThOCH3 ) using a potentiostatic pulse method...
July 30, 2018: Biosensors & Bioelectronics
Prosenjit Pyne, Masrure Alam, Moidu Jameela Rameez, Subhrangshu Mandal, Abhijit Sar, Nibendu Mondal, Utsab Debnath, Boby Mathew, Anup Kumar Misra, Amit Kumar Mandal, Wriddhiman Ghosh
The SoxXAYZB(CD)2 -mediated pathway of bacterial sulfur-chemolithotrophy explains the oxidation of thiosulfate, sulfide, sulfur and sulfite but not tetrathionate. Advenella kashmirensis, which oxidizes tetrathionate to sulfate, besides forming it as an intermediate during thiosulfate oxidation, possesses a soxCDYZAXOB operon. Knock-out mutations proved that only SoxBCD is involved in A. kashmirensis tetrathionate oxidation, whereas thiosulfate-to-tetrathionate conversion is Sox independent. Expression of two glutathione metabolism-related proteins increased under chemolithotrophic conditions, as compared to the chemoorganotrophic one...
April 18, 2018: Molecular Microbiology
Sarita Nehra, Varun Bhardwaj, Anju Bansal, Pronobesh Chattopadhyay, Deepika Saraswat
This study investigates the therapeutic effect of a nanocurcumin formulation (NCF) containing nanocurcumin (NC) and pyrroloquinoline quinone (PQQ) on ameliorating hypoxia-induced stress in hypertrophied primary human ventricular cardiomyocytes (HVCM) under hypoxic conditions, as validated in a Sprague-Dawley rat model of chronic hypobaric hypoxia (cHH)-induced right ventricular hypertrophy (RVH). Based on our previous findings, here, we analyzed the improvement in the protective efficacy of NCF against mitochondrial damage...
December 1, 2017: Experimental & Molecular Medicine
Anikó Várnai, Kiwamu Umezawa, Makoto Yoshida, Vincent G H Eijsink
Fungi secrete a set of glycoside hydrolases and oxidoreductases, including lytic polysaccharide monooxygenases (LPMOs), for the degradation of plant polysaccharides. LPMOs catalyze the oxidative cleavage of glycosidic bonds after activation by an external electron donor. So far, only flavin-dependent oxidoreductases (from the auxiliary activity family AA3) have been shown to activate LPMOs. Here we present LPMO activation by a pyrroloquinoline-quinone (PQQ)-dependent pyranose dehydrogenase (PDH) from Coprinopsis cinerea , Cc PDH, the founding member of the recently discovered auxiliary activity family AA12...
March 30, 2018: Applied and Environmental Microbiology
Jiaying Qiu, Qingqing Fang, Tongtong Xu, Changyue Wu, Lai Xu, Lingbin Wang, Xiaoming Yang, Shu Yu, Qi Zhang, Fei Ding, Hualin Sun
Skeletal muscle atrophy occurs under various conditions, such as disuse, denervation, fasting, aging, and various diseases. Although the underlying molecular mechanisms are still not fully understood, skeletal muscle atrophy is closely associated with reactive oxygen species (ROS) overproduction. In this study, we aimed to investigate the involvement of ROS in skeletal muscle atrophy from the perspective of gene regulation, and further examine therapeutic effects of antioxidants on skeletal muscle atrophy. Microarray data showed that the gene expression of many positive regulators for ROS production were up-regulated and the gene expression of many negative regulators for ROS production were down-regulated in mouse soleus muscle atrophied by denervation (sciatic nerve injury)...
2018: Frontiers in Physiology
Yang Zhou, Honghui Zhu, Qing Yao
Phosphorus (P) cycling is a fundamental process driven by microorganisms, and plants can regulate P cycling directly or via their influence on the soil microbial community. However, the differential P cycling patterns associated with legumes and grass are largely unknown. Therefore, we investigated the microbial community involved in P cycling in subtropical soil grown with stylo (Stylosanthes guianensis, legume) or bahiagrass (Paspalum notatum, grass) using metagenomic sequencing. P fractionation indicated that sparingly soluble inorganic P (Pi) accounted for approximately 75% of P pool...
March 24, 2018: Environmental Microbiology Reports
Linchun Wen, Xiyan Lu, Rui Wang, Xiaowei Jin, Liqiang Hu, Chuanwen You
Pyrroloquinoline quinone (PQQ) has been reported to contribute to cancer cell apoptosis and death; however, little is known of its underlying mechanisms. The present study was designed to investigate the role of PQQ in chondrosarcoma cell apoptosis and the underlying mechanism. A cell cytotoxicity assay was used to detect cell death; flow cytometry analysis was also performed to determine cell apoptosis and intracellular reactive oxygen species (ROS). Biochemical methods were employed to detect the activity and the expression of superoxide dismutase (SOD)1, SOD2 and glutathione...
May 2018: Molecular Medicine Reports
Jacob E Friedman, Evgenia Dobrinskikh, Alba Alfonso-Garcia, Alexander Fast, Rachel C Janssen, Taylor K Soderborg, Aimee L Anderson, Julie A Reisz, Angelo D'Alessandro, Daniel N Frank, Charles E Robertson, Becky A de la Houssaye, Linda K Johnson, David J Orlicky, Xiaoxin X Wang, Moshe Levi, Eric O Potma, Karim C El Kasmi, Karen R Jonscher
Increasingly, evidence suggests that exposure to maternal obesity creates an inflammatory environment in utero , exerting long-lasting postnatal signatures on the juvenile innate immune system and microbiome that may predispose offspring to development of fatty liver disease. We found that exposure to a maternal Western-style diet (WD) accelerated fibrogenesis in the liver of offspring and was associated with early recruitment of proinflammatory macrophages at 8-12 weeks and microbial dysbiosis as early as 3 weeks of age...
March 2018: Hepatology Communications
Thinh-Phat Cao, Jin Myung Choi, Si Wouk Kim, Sung Haeng Lee
The first crystal structure of a pyrroloquinoline quinone (PQQ)-dependent methanol dehydrogenase (MDH) from a marine methylotrophic bacterium, Methylophaga aminisulfidivorans MPT (MDH Mas ), was determined at 1.7 Å resolution. The active form of MDH Mas (or MDHI Mas ) is a heterotetrameric α2 β2 , where each β-subunit assembles on one side of each of the α-subunits, in a symmetrical fashion, so that two β-subunits surround the two PQQ-binding pockets on the α-subunits. The active site consists of a PQQ molecule surrounded by a β-propeller fold for each α-subunit...
April 2018: Journal of Microbiology / the Microbiological Society of Korea
Toshiharu Yakushi, Yuka Terada, Seishiro Ozaki, Naoya Kataoka, Yoshihiko Akakabe, Osao Adachi, Minenosuke Matsutani, Kazunobu Matsushita
Membrane-bound, pyrroloquinoline quinone (PQQ)-dependent glycerol dehydrogenase (GLDH, or polyol dehydrogenase) of Gluconobacter sp. oxidizes various secondary alcohols to produce the corresponding ketones, such as oxidation of D-sorbitol to L-sorbose in vitamin C production. Substrate specificity of GLDH is considered limited to secondary alcohols in the D-erythro configuration at the next to the last carbon. Here, we suggest that L-ribose, D- and L-lyxoses, and L-tagatose are also substrates of GLDH, but these sugars do not meet the substrate specificity rule of GLDH...
April 2018: Applied Microbiology and Biotechnology
Yuanqing Huang, Ning Chen, Dengshun Miao
To investigate whether pyrroloquinoline quinine (PQQ) plays an important role in rescuing Bmi-1-/- mice induced developmental disorders of teeth and mandible by regulating oxidative stress. We fed Bmi-1-/- mice a diet supplemented with PQQ (BKO+PQQ), BKO mice with normal diet (BKO) and wild type mice with normal diet (WT) as controls. We compared the differences of dental, mandibular phenotype by means of X-ray photography, micro CT scanning and three-dimensional reconstruction, HE staining, histochemistry, immunoistohemistry, TUNEL staining, Western blot and Flow cytometry in three groups of animals...
2018: American Journal of Translational Research
Toshiharu Yakushi, Kazutaka Komatsu, Minenosuke Matsutani, Naoya Kataoka, Alisa S Vangnai, Hirohide Toyama, Osao Adachi, Kazunobu Matsushita
Gluconobacter oxydans produces 3-dehydroquinate by oxidation of quinate through a reaction catalyzed by the quinate dehydrogenase (QDH), membrane-bound, pyrroloquinoline quinone (PQQ)-dependent dehydrogenase. We previously reported the nucleotide and deduced amino acid sequence of QDH and constructed a heterologous expression system of QDH in Pseudomonas sp. (A.S. Vangnai, W. Promden, W. De-Eknamkul, K. Matsushita, H. Toyama, Biochemistry (Moscow) 75:452-459, 2010). Through this study, we aim to update the sequences of QDH and improve the heterologous expression of QDH in Gluconobacter strains using a broad-host-range plasmid...
May 2018: Protein Expression and Purification
Matthias Wehrmann, Janosch Klebensberger
Due to their ability for direct electron transfer to electrodes, the utilization of rare earth metals as cofactor, and their periplasmic localization, pyrroloquinoline quinone-dependent alcohol dehydrogenases (PQQ-ADHs) represent an interesting class of biocatalysts for various biotechnological applications. For most biocatalysts protein stability is crucial, either to increase the performance of the protein under a given process condition or to maximize robustness of the protein towards mutational manipulations, which are often needed to enhance or introduce a functionality of interest...
March 2018: Microbial Biotechnology
Natsaran Saichana, Katsuyuki Tanizawa, Hiroshi Ueno, Jiří Pechoušek, Petr Novák, Jitka Frébortová
PqqE is a radical S -adenosyl-l-methionine (SAM) enzyme that catalyzes the initial reaction of pyrroloquinoline quinone (PQQ) biosynthesis. PqqE belongs to the SPASM (subtilosin/PQQ/anaerobic sulfatase/mycofactocin maturating enzymes) subfamily of the radical SAM superfamily and contains multiple Fe - S clusters. To characterize the Fe - S clusters in PqqE from Methylobacterium extorquens AM1, Cys residues conserved in the N-terminal signature motif (CX3 CX2 C) and the C-terminal seven-cysteine motif (CX9-15 GX4 CX n CX2 CX5 CX3 CX n C; n  = an unspecified number) were individually or simultaneously mutated into Ser...
December 2017: FEBS Open Bio
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