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https://www.readbyqxmd.com/read/28448745/medium-optimization-for-pyrroloquinoline-quinone-pqq-production-by-methylobacillus-sp-zju323-using-response-surface-methodology-and-artificial-neural-network-genetic-algorithm
#1
Peilian Wei, Zhenjun Si, Yao Lu, Qingfei Yu, Lei Huang, Zhinan Xu
Methylobacillus sp. zju323 was adopted to improve the biosynthesis of pyrroloquinoline quinone (PQQ) by systematic optimization of the fermentation medium. The Plackett-Burman design (PBD) was implemented to screen for the key medium components for the PQQ production. CoCl2 · 6H2O, ρ-amino benzoic acid (PABA), and MgSO4 · 7H2O were found capable of enhancing the PQQ production most significantly. A five level-three factor central composite design (CCD) was employed to investigate the direct and interactive effects of these variables...
April 27, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28413833/beneficial-effects-of-a-pyrroloquinolinequinone-containing-dietary-formulation-on-motor-deficiency-cognitive-decline-and-mitochondrial-dysfunction-in-a-mouse-model-of-alzheimer-s-disease
#2
Darrell Sawmiller, Song Li, Takashi Mori, Ahsan Habib, David Rongo, Vedad Delic, Patrick C Bradshaw, R Douglas Shytle, Cyndy Sanberg, Paula Bickford, Jun Tan
Alzheimer's disease (AD), a progressive neurodegenerative disorder, is linked to oxidative stress, altered amyloid precursor protein (APP) proteolysis, tau hyperphosphorylation and the accumulation of amyloid-β (Aβ) plaques and neurofibrillary tangles (NFT). A growing body of evidence suggests that mitochondrial dysfunction can be a key promoter of all of these pathologies and predicts that restoration of mitochondrial function might be a potential therapeutic strategy for AD. Therefore, in the present study, we tested the beneficial effect of a nutraceutical formulation Nutrastem II (Nutra II), containing NT020 (a mitochondrial restorative and antioxidant proprietary formulation) and pyrroloquinolinequinone (PQQ, a stimulator of mitochondria biogenesis) in 5XFAD transgenic mice...
April 2017: Heliyon
https://www.readbyqxmd.com/read/28407913/pyrroloquinoline-quinone-from-gluconobacter-oxydans-fermentation-broth-enhances-superoxide-anion-scavenging-capacity-of-cu-zn-sod
#3
Ke Ma, Jun-Zhu Cui, Jian-Bin Ye, Xian-Mei Hu, Ge-Li Ma, Xue-Peng Yang
A bioassay-guided fractionation of extract from Gluconobacter oxydans fermentation broth afforded Compound 1, which was identified as pyrroloquinoline quinone (PQQ) by spectroscopic methods. PQQ has been shown to enhance the superoxide anion-scavenging capacity significantly for Cu/Zn-SOD. To illustrate the mechanism, the interaction between PQQ and Cu/Zn-SOD was investigated. The multiple binding sites involving hydrogen bonds and van der Waals force between PQQ and Cu/Zn-SOD were revealed by isothermal titration calorimetry...
September 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28386349/pyrroloquinoline-quinone-prevents-testosterone-deficiency-induced-osteoporosis-by-stimulating-osteoblastic-bone-formation-and-inhibiting-osteoclastic-bone-resorption
#4
Xuan Wu, Jie Li, Hengwei Zhang, Hui Wang, Guoyong Yin, Dengshun Miao
Accumulating evidences suggest that oxidative stress caused and deteriorated the aging related osteoporosis and pyrroloquinoline quinone (PQQ) is a powerful antioxidant. However, it is unclear whether PQQ can prevent testosterone deficiency-induced osteoporosis. In this study, the orchidectomized (ORX) mice were supplemented in diet with/without PQQ for 48 weeks, and compared with each other and with sham mice. Results showed that bone mineral density, trabecular bone volume, collagen deposition and osteoblast number were decreased significantly in ORX mice compared with shame mice, whereas PQQ supplementation largely prevented these alterations...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/28374050/enhanced-fed-batch-production-of-pyrroloquinoline-quinine-in-methylobacillus-sp-cctcc-m2016079-with-a-two-stage-ph-control-strategy
#5
Zhenjun Si, David Machaku, Peilian Wei, Lei Huang, Jin Cai, Zhinan Xu
The effects of pH control strategy and fermentative operation modes on the biosynthesis of pyrroloquinoline quinine (PQQ) were investigated systematically with Methylobacillus sp. CCTCC M2016079 in the present work. Firstly, the shake-flask cultivations and benchtop fermentations at various pH values ranging from 5.3 to 7.8 were studied. Following a kinetic analysis of specific cell growth rate (μ x ) and specific PQQ formation rate (μ p ), the discrepancy in optimal pH values between cell growth and PQQ biosynthesis was observed, which stimulated us to develop a novel two-stage pH control strategy...
April 3, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28344084/a-rapid-procedure-for-the-in-situ-assay-of-periplasmic-pqq-dependent-methanol-dehydrogenase-in-intact-single-bacterial-colonies
#6
Venkata Ramana Vemuluri, Shreya Shaw, Caroline Autenrieth, Robin Ghosh
Mechanistic details of methanol oxidation catalyzed by the periplasmically-located pyrroloquinoline quinone-dependent methanol dehydrogenase of methylotrophs can be elucidated using site-directed mutants. Here, we present an in situ colony assay of methanol dehydrogenase, which allows robotic screening of large populations of intact small colonies, and regrowth of colonies for subsequent analysis.
March 23, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28283693/genetically-engineered-escherichia-coli-nissle-1917-synbiotic-counters-fructose-induced-metabolic-syndrome-and-iron-deficiency
#7
Archana Somabhai Chaudhari, Ruma Raghuvanshi, G Naresh Kumar
Consumption of fructose leads to metabolic syndrome, but it is also known to increase iron absorption. Present study investigates the effect of genetically modified Escherichia coli Nissle 1917 (EcN) synbiotic along with fructose on non-heme iron absorption. Charles foster rats weighing 150-200 g were fed with iron-deficient diet for 2 months. Probiotic treatment of EcN (pqq) and EcN (pqq-glf-mtlK) was given once per week, 10(9) cells after 2 months with fructose in drinking water. Iron levels, blood, and liver parameters for oxidative stress, hyperglycemia, and dyslipidemia were estimated...
March 10, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28155960/connecting-quantum-dots-with-enzymes-mediator-based-approaches-for-the-light-directed-read-out-of-glucose-and-fructose-oxidation
#8
M Riedel, N Sabir, F W Scheller, W J Parak, F Lisdat
The combination of the biocatalytic features of enzymes with the unique physical properties of nanoparticles in a biohybrid system provides a promising approach for the development of advanced bioelectrocatalytic devices. This study describes the construction of photoelectrochemical signal chains based on CdSe/ZnS quantum dot (QD) modified gold electrodes as light switchable elements, and low molecular weight redox molecules for the combination with different biocatalysts. Photoelectrochemical and photoluminescence experiments verify that electron transfer can be achieved between the redox molecules hexacyanoferrate and ferrocene, and the QDs under illumination...
February 3, 2017: Nanoscale
https://www.readbyqxmd.com/read/28124619/the-effect-of-pyrroloquinoline-quinone-on-apoptosis-and-autophagy-in-traumatic-brain-injury
#9
Pengju Zhang, Yongqi Ye, Yuhang Qian, Baoxin Yin, Jianmei Zhao, Shunxin Zhu, Li Zhang, Meijuan Yan
BACKGROUND: Pyrroloquinoline quinoe is an anionic, water-soluble compound with antioxidant characteristic. The role of pyrroloquinoline quinoe in pharmacology and nutrition has attracted wide attention of researchers. Although a few experiments have confirmed that pyrroloquinoline quinoe plays an obvious effective role in neuroprotection. There are little reports about the effect of pyrroloquinoline quinoe in traumatic brain injury. Traumatic brain injury is one of the leading causes of adult disability and death in the world...
January 24, 2017: CNS & Neurological Disorders Drug Targets
https://www.readbyqxmd.com/read/28115068/the-role-of-oxidative-stress-in-dna-damage-in-pancreatic-%C3%AE-cells-induced-by-di-2-ethylhexyl-phthalate
#10
Yan She, Liping Jiang, Liangliang Zheng, Heming Zuo, Min Chen, Xiance Sun, Qiujuan Li, Chengyan Geng, Guang Yang, Lijie Jiang, Xiaofang Liu
Di(2-ethyhexyl) phthalate (DEHP) is commonly used as a plasticizer, which loosely binds to plastic materials and easily leaches out of these products and enters into the environment. Exposure to DEHP can impair pancreatic beta cells (INS-1 cells)function, which is associated with Insulin Resistance (IR) and type 2 diabetes. However, the mechanism of how DEHP leads to Insulin Resistance is unknown. Our results showed that the cell viability of INS-1 cells exposed to DEHP (0-1600 μM) were decreased in a concentration-dependent manner...
March 1, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28105098/radioprotective-effects-of-pyrroloquinoline-quinone-on-parotid-glands-in-c57bl-6j-mice
#11
Yuanqing Huang, Ning Chen, Dengshun Miao
The aim of the present study was to investigate whether pyrroloquinoline quinine (PQQ) serve a radioprotective role in parotid gland damage induced by total body irradiation (TBI) in C57BL/6J mice. A total of 15 female 8-week-old C57BL/6J mice were randomly assigned into three treatment groups: i) Untreated control (no irradiation); ii) 4 gray (Gy) X-ray irradiation; iii) 4 Gy X-ray irradiation with additional dietary PQQ (4 mg PQQ/kg in normal diet). Each group included five mice. After 4 weeks, all animals were collected for evaluating the phenotype, body weight, pathological and biochemical parameters...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28063921/tricalcium-phosphate-solubilization-and-nitrogen-fixation-by-newly-isolated-aneurinibacillus-aneurinilyticus-ckmv1-from-rhizosphere-of-valeriana-jatamansi-and-its-growth-promotional-effect
#12
Anjali Chauhan, Shiwani Guleria, Praveen P Balgir, Abhishek Walia, Rishi Mahajan, Preeti Mehta, Chand Karan Shirkot
Aneurinibacillus aneurinilyticus strain CKMV1 was isolated from rhizosphere of Valeriana jatamansi and possessed multiple plant growth promoting traits like production of phosphate solubilization (260mg/L), nitrogen fixation (202.91nmolethylenemL(-1)h(-1)), indole-3-acetic acid (IAA) (8.1μg/mL), siderophores (61.60%), HCN (hydrogen cyanide) production and antifungal activity. We investigated the ability of isolate CKMV1 to solubilize insoluble P via mechanism of organic acid production. High-performance liquid chromatography (HPLC) study showed that isolate CKMV1 produced mainly gluconic (1...
April 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28007783/early-pqq-supplementation-has-persistent-long-term-protective-effects-on-developmental-programming-of-hepatic-lipotoxicity-and-inflammation-in-obese-mice
#13
Karen R Jonscher, Michael S Stewart, Alba Alfonso-Garcia, Brian C DeFelice, Xiaoxin X Wang, Yuhuan Luo, Moshe Levi, Margaret J R Heerwagen, Rachel C Janssen, Becky A de la Houssaye, Ellen Wiitala, Garrett Florey, Raleigh L Jonscher, Eric O Potma, Oliver Fiehn, Jacob E Friedman
Nonalcoholic fatty liver disease (NAFLD) is widespread in adults and children. Early exposure to maternal obesity or Western-style diet (WD) increases steatosis and oxidative stress in fetal liver and is associated with lifetime disease risk in the offspring. Pyrroloquinoline quinone (PQQ) is a natural antioxidant found in soil, enriched in human breast milk, and essential for development in mammals. We investigated whether a supplemental dose of PQQ, provided prenatally in a mouse model of diet-induced obesity during pregnancy, could protect obese offspring from progression of NAFLD...
April 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/27951709/amphiphilic-polymer-mediators-promoting-electron-transfer-on-bioanodes-with-pqq-dependent-glucose-dehydrogenase
#14
Yasuo Nakashima, Norihiro Mizoshita, Hiromitsu Tanaka, Yuichiro Nakaoki
Redox-active phenazinium salts bonded to amphiphilic polymer backbones are demonstrated to function as high-performance electron-transfer mediators in enzymatic bioanodes applicable to biofuel cells. The redox-active moieties could be easily tethered to the electrodes by physical adsorption of the hydrophobic regions of the polymer backbones onto the electrode surface. On the other hand, long hydrophilic chains were essential to ensure high mobility of the redox-active moieties in aqueous solutions and to enhance their electron-transfer properties...
December 13, 2016: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/27876710/enhancement-of-1-3-dihydroxyacetone-production-from-gluconobacter-oxydans-by-combined-mutagenesis
#15
Xi Lin, Sha Liu, Guangrong Xie, Jing Chen, Penghua Li, Jianhua Chen
Wild strain L-6 was subjected to combined mutagenesis, including UV irradiation, atmospheric and room temperature plasma, and ion beam implantation, to increase the yield of 1,3-dihydroxyacetone (DHA). With application of a high-throughput screening method, mutant Gluconobacter oxydans I-2-239 with a DHA productivity of 103.5 g/l in flask-shake fermentation was finally obtained with the starting glycerol concentration of 120 g/l, which was 115.7% higher than the wild strain. The cultivation time also decreased from 54 h to 36 h...
November 28, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27849146/membrane-bound-glycerol-dehydrogenase-catalyzes-oxidation-of-d-pentonates-to-4-keto-d-pentonates-d-fructose-to-5-keto-d-fructose-and-d-psicose-to-5-keto-d-psicose
#16
Yoshitaka Ano, Roque A Hours, Yoshihiko Akakabe, Naoya Kataoka, Toshiharu Yakushi, Kazunobu Matsushita, Osao Adachi
A novel oxidation of D-pentonates to 4-keto-D-pentonates was analyzed with Gluconobacter thailandicus NBRC 3258. D-Pentonate 4-dehydrogenase activity in the membrane fraction was readily inactivated by EDTA and it was reactivated by the addition of PQQ and Ca(2+). D-Pentonate 4-dehydrogenase was purified to two different subunits, 80 and 14 kDa. The absorption spectrum of the purified enzyme showed no typical absorbance over the visible regions. The enzyme oxidized D-pentonates to 4-keto-D-pentonates at the optimum pH of 4...
February 2017: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/27836608/an-improved-glycerol-biosensor-with-an-au-fes-nad-glycerol-dehydrogenase-anode
#17
Aishwarya Mahadevan, Sandun Fernando
An improved glycerol biosensor was developed via direct attachment of NAD(+)-glycerol dehydrogenase coenzyme-apoenzyme complex onto supporting gold electrodes, using novel inorganic iron (II) sulfide (FeS)-based single molecular wires. Sensing performance factors, i.e., sensitivity, a detection limit and response time of the FeS and conventional pyrroloquinoline quinone (PQQ)-based biosensor were evaluated by dynamic constant potential amperometry at 1.3V under non-buffered conditions. For glycerol concentrations ranging from 1 to 25mM, a 77% increase in sensitivity and a 53% decrease in detection limit were observed for the FeS-based biosensor when compared to the conventional PQQ-based counterpart...
June 15, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27805779/a-nernstian-biosupercapacitor
#18
Dmitry Pankratov, Felipe Conzuelo, Piyanut Pinyou, Sabine Alsaoub, Wolfgang Schuhmann, Sergey Shleev
We propose the very first "Nernstian biosupercapacitor", a biodevice based on only one redox polymer: poly(vinyl imidazole-co-allylamine)[Os(bpy)2 Cl], and two biocatalysts. At the bioanode PQQ-dependent glucose dehydrogenase reduces the Os(3+) moieties at the polymer to Os(2+) shifting the Nernst potential of the Os(3+) /Os(2+) redox couple to negative values. Concomitantly, at the biocathode the reduction of O2 by means of bilirubin oxidase embedded in the same redox polymer leads to the oxidation of Os(2+) to Os(3+) shifting the Nernst potential to higher values...
December 5, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27760187/genetically-engineered-escherichia-coli-nissle-1917-synbiotics-reduce-metabolic-effects-induced-by-chronic-consumption-of-dietary-fructose
#19
Chaudhari Archana Somabhai, Ruma Raghuvanshi, G Nareshkumar
AIMS: To assess protective efficacy of genetically modified Escherichia coli Nissle 1917 (EcN) on metabolic effects induced by chronic consumption of dietary fructose. MATERIALS AND METHODS: EcN was genetically modified with fructose dehydrogenase (fdh) gene for conversion of fructose to 5-keto-D-fructose and mannitol-2-dehydrogenase (mtlK) gene for conversion to mannitol, a prebiotic. Charles foster rats weighing 150-200 g were fed with 20% fructose in drinking water for two months...
2016: PloS One
https://www.readbyqxmd.com/read/27638737/-1-h-13-c-and-15-n-resonance-assignments-and-secondary-structure-information-for-methylobacterium-extorquens-pqqd-and-the-complex-of-pqqd-with-pqqa
#20
Robert L Evans, John A Latham, Judith P Klinman, Carrie M Wilmot, Youlin Xia
The ribosomally synthesized and post-translationally modified peptide (RiPP), pyrroloquinoline quinone (PQQ), is a dehydrogenase cofactor synthesized by, but not exclusively used by, certain prokaryotes. RiPPs represent a rapidly expanding and diverse class of natural products-many of which have therapeutic potential-and the biosynthetic pathways for these are gaining attention. Five gene products from the pqq operon (PqqA, PqqB, PqqC, PqqD, and PqqE) are essential for PQQ biosynthesis. The substrate is the peptide PqqA, which is presented to the radical SAM enzyme PqqE by the small protein PqqD...
October 2016: Biomolecular NMR Assignments
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