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https://www.readbyqxmd.com/read/29220151/integration-of-enzymes-in-polyaniline-sensitized-3d-inverse-opal-tio2-architectures-for-light-driven-biocatalysis-and-light-to-current-conversion
#1
Marc Riedel, Fred Lisdat
Inspired by natural photosynthesis, coupling of artificial light-sensitive entities with biocatalysts in a biohybrid format can result in advanced photobioelectronic systems. Herein, we report on the integration of sulfonated polyanilines (PMSA1) and PQQ glucose dehydrogenase (PQQ-GDH) into inverse opal TiO2 (IO-TiO2) electrodes. While PMSA1 introduces sensitivity for visible light into the biohybrid architecture and ensures the efficient wiring between the IO-TiO2 electrode and the biocatalytic entity, PQQ-GDH provides the catalytic activity for the glucose oxidation and therefore feeds the light-driven reaction with electrons for an enhanced light-to-current conversion...
December 8, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29185751/ca2-switchable-glucose-dehydrogenase-associated-with-electrochemical-electronic-interfaces-applications-to-signal-controlled-power-production-and-biomolecular-release
#2
Ashkan Koushanpour, María Gamella, Zhong Guo, Elham Honarvarfard, Arshak Poghossian, Michael J Schoening, Kirill Alexandrov, Evgeny Katz
An artificial Ca2+-regulated PQQ glucose dehydrogenase (PQQ-GDH) enzyme was electrically connected to conducting electrodes and semiconductor interfaces. Direct electron transfer from the enzyme to the conducting electrode support was stimulated by the addition of Ca2+ cations resulting in reversible enzyme activation. A signal-switchable biofuel cell and biomolecular release have been realized using the Ca2+-activated enzyme immobilized on conducting electrodes. Interfacing the signal-switchable enzyme with a semiconductor chip allowed electronic read out of the enzyme ON-OFF states...
November 29, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/29185343/pyrroloquinoline-quinone-a-redox-active-o-quinone-stimulates-mitochondrial-biogenesis-by-activating-sirt1-pgc-1%C3%AE-signaling-pathway
#3
Kazuhiro Saihara, Ryosuke Kamikubo, Kazuto Ikemoto, Koji Uchida, Mitsugu Akagawa
Pyrroloquinoline quinone (PQQ), a redox-active o-quinone found in various foods and mammalian tissues, has received increasing attention in a number of health benefits due to its ability to enhance mitochondrial biogenesis. However, its underlying molecular mechanism remains incompletely understood. We have now established that the exposure of mouse NIH/3T3 fibroblasts to a physiologically relevant concentration of PQQ significantly stimulates the mitochondrial biogenesis. The exposure of NIH/3T3 cells to 10-100 nM PQQ for 48 h resulted in increased Mitotracker staining, the mitochondrial DNA content, and the mitochondrially-encoded cytochrome c oxidase subunit 1 (MTCO1) protein...
November 29, 2017: Biochemistry
https://www.readbyqxmd.com/read/29148251/the-enzymatic-detoxification-of-the-mycotoxin-deoxynivalenol-identification-of-depa-from-the-don-epimerization-pathway
#4
Jason Carere, Yousef I Hassan, Dion Lepp, Ting Zhou
The biological detoxification of mycotoxins, including deoxynivalenol (DON), represents a very promising approach to address the challenging problem of cereal grain contamination. The recent discovery of Devosia mutans 17-2-E-8 (Devosia spp. 17-2-E-8), a bacterial isolate capable of transforming DON to the non-toxic stereoisomer 3-epi-deoxynivalenol, along with earlier reports of bacterial species capable of oxidizing DON to 3-keto-DON, has generated interest in the possible mechanism and enzyme(s) involved...
November 17, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/29118900/effect-and-mechanism-of-pyrroloquinoline-quinone-on-anti-osteoporosis-in-bmi-1-knockout-mice-anti-oxidant-effect-of-pyrroloquinoline-quinone
#5
Yuanqing Huang, Ning Chen, Dengshun Miao
Pyrroloquinoline quinone (PQQ), considered as an ROS scavenger,could protect mitochondrial activity from damage of oxidative stress. To determine the role of PQQ supplement in rescuing long bone osteoporosis in Bmi-1(-/-) mice. We fed Bmi-1 knockout mice a diet supplemented with PQQ (BKO+PQQ), BKO mice with normal diet (BKO) and wild type mice with normal diet (WT) as controls. We compared the differences of skeletal phenotype by means of imaging, histopathological and molecular biology methods in three groups of animals...
2017: American Journal of Translational Research
https://www.readbyqxmd.com/read/29059797/a-self-powered-glucose-biosensor-based-on-pyrolloquinoline-quinone-glucose-dehydrogenase-and-bilirubin-oxidase-operating-under-physiological-conditions
#6
Tanmay Kulkarni, Gymama Slaughter
A novel biosensing system capable of simultaneously sensing glucose and powering portable electronic devices such as a digital glucometer is described. The biosensing system consists of enzymatic glucose biofuel cell bioelectrodes functionalized with pyrolloquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BOD) at the bioanode and biocathode, respectively. A dual-stage power amplification circuit is integrated with the single biofuel cell to amplify the electrical power generated. In addition, a capacitor circuit was incorporated to serve as the transducer for sensing glucose...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/29049946/rechargeable-flexible-and-mediator-free-biosupercapacitor-based-on-transparent-ito-nanoparticle-modified-electrodes-acting-in-%C3%A2%C2%B5m-glucose-containing-buffers
#7
Tim Bobrowski, Elena González Arribas, Roland Ludwig, Miguel D Toscano, Sergey Shleev, Wolfgang Schuhmann
We present a transparent and flexible self-charging biosupercapacitor based on an optimised mediator- and membrane-free enzymatic glucose/oxygen biofuel cell. Indium tin oxide (ITO) nanoparticles were spray-coated on transparent conducting ITO supports resulting in a flocculent, porous and nanostructured electrode surface. By this, high capacitive currents caused by an increased electrochemical double layer as well as enhanced catalytic currents due to a higher number of immobilised enzyme molecules were obtained...
October 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28932219/improving-acetic-acid-production-by-over-expressing-pqq-adh-in-acetobacter-pasteurianus
#8
Xuefeng Wu, Hongli Yao, Lili Cao, Zhi Zheng, Xiaoju Chen, Min Zhang, Zhaojun Wei, Jieshun Cheng, Shaotong Jiang, Lijun Pan, Xingjiang Li
Pyrroquinoline quinone-dependent alcohol dehydrogenase (PQQ-ADH) is a key enzyme in the ethanol oxidase respiratory chain of acetic acid bacteria (AAB). To investigate the effect of PQQ-ADH on acetic acid production by Acetobacter pasteurianus JST-S, subunits I (adhA) and II (adhB) of PQQ-ADH were over-expressed, the fermentation parameters and the metabolic flux analysis were compared in the engineered strain and the original one. The acetic acid production was improved by the engineered strain (61.42 g L(-1)) while the residual ethanol content (4...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28883836/the-effect-of-pyrroloquinoline-quinone-on-the-expression-of-wisp1-in-traumatic-brain-injury
#9
Yongqi Ye, Pengju Zhang, Yuhang Qian, Baoxin Yin, Meijuan Yan
WISP1, as a member of the CCN4 protein family, has cell protective effects of promoting cell proliferation and inhibiting cell apoptosis. Although some studies have confirmed that WISP1 is concerned with colon cancer and lung cancer, there is little report about the influence of WISP1 in traumatic brain injury. Here, we found that the expression of WISP1 mRNA and protein decreased at 3 d and then increased at 5 d after traumatic brain injury (TBI). Meanwhile, immunofluorescence demonstrated that there was little colocation of WISP1 with GFAP, Iba1, and WISP1 colocalized with NeuN partly...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28709697/effects-of-p-limitation-and-molecules-from-peanut-root-exudates-on-pqqe-gene-expression-and-pqq-promoter-activity-in-the-phosphate-solubilizing-strain-serratia-sp-s119
#10
Liliana M Ludueña, Maria S Anzuay, Cynthia Magallanes-Noguera, Maria L Tonelli, Fernando J Ibañez, Jorge G Angelini, Adriana Fabra, Matthew McIntosh, Tania Taurian
The mineral phosphate-solubilizing phenotype in bacteria is attributed predominantly to secretion of gluconic acid produced by oxidation of glucose by the glucose dehydrogenase enzyme and its cofactor, pyrroloquinoline quinone. This study analyzes pqqE gene expression and pqq promoter activity in the native phosphate-solubilizing bacterium Serratia sp S119 growing under P-limitation, and in the presence of root exudates obtained from peanut plants, also growing under P-limitation. Results indicated that Serratia sp...
October 2017: Research in Microbiology
https://www.readbyqxmd.com/read/28676501/lifespan-extension-by-peroxidase-and-dual-oxidase-mediated-ros-signaling-through-pyrroloquinoline-quinone-in-c-elegans
#11
Hiroyuki Sasakura, Hiroki Moribe, Masahiko Nakano, Kazuto Ikemoto, Kosei Takeuchi, Ikue Mori
Reactive oxygen species (ROS), originally characterized based on their harmful effects on cells or organisms, are now recognized as important signal molecules regulating various biological processes. In particular, low levels of ROS released from mitochondria extend lifespan. Here, we identified a novel mechanism of generating appropriate levels of ROS at the plasma membrane through a peroxidase and dual oxidase (DUOX) system, which could extend lifespan in Caenorhabditis elegans A redox co-factor, pyrroloquinoline quinone (PQQ), activates the C...
August 1, 2017: Journal of Cell Science
https://www.readbyqxmd.com/read/28672774/combinatorial-therapy-of-exercise-preconditioning-and-nanocurcumin-formulation-supplementation-improves-cardiac-adaptation-under-hypobaric-hypoxia
#12
Sarita Nehra, Varun Bhardwaj, Anju Bansal, Deepika Saraswat
BACKGROUND: Chronic hypobaric hypoxia (cHH) mediated cardiac insufficiencies are associated with pathological damage. Sustained redox stress and work load are major causative agents of cardiac insufficiencies under cHH. Despite the advancements made in pharmacological (anti-oxidants, vasodilators) and non-pharmacological therapeutics (acclimatization strategies and schedules), only partial success has been achieved in improving cardiac acclimatization to cHH. This necessitates the need for potent combinatorial therapies to improve cardiac acclimatization at high altitudes...
September 26, 2017: Journal of Basic and Clinical Physiology and Pharmacology
https://www.readbyqxmd.com/read/28655819/functional-role-of-lanthanides-in-enzymatic-activity-and-transcriptional-regulation-of-pyrroloquinoline-quinone-dependent-alcohol-dehydrogenases-in-pseudomonas-putida-kt2440
#13
Matthias Wehrmann, Patrick Billard, Audrey Martin-Meriadec, Asfaw Zegeye, Janosch Klebensberger
The oxidation of alcohols and aldehydes is crucial for detoxification and efficient catabolism of various volatile organic compounds (VOCs). Thus, many Gram-negative bacteria have evolved periplasmic oxidation systems based on pyrroloquinoline quinone-dependent alcohol dehydrogenases (PQQ-ADHs) that are often functionally redundant. Here we report the first description and characterization of a lanthanide-dependent PQQ-ADH (PedH) in a nonmethylotrophic bacterium based on the use of purified enzymes from the soil-dwelling model organism Pseudomonas putida KT2440...
June 27, 2017: MBio
https://www.readbyqxmd.com/read/28578007/sensitivity-of-hipsc-derived-neural-stem-cells-nsc-to-pyrroloquinoline-quinone-depends-on-their-developmental-stage
#14
J Augustyniak, J Lenart, M Zychowicz, G Lipka, P Gaj, M Kolanowska, P P Stepien, L Buzanska
Pyrroloquinoline quinone (PQQ) is a factor influencing on the mitochondrial biogenesis. In this study the PQQ effect on viability, total cell number, antioxidant capacity, mitochondrial biogenesis and differentiation potential was investigated in human induced Pluripotent Stem Cells (iPSC) - derived: neural stem cells (NSC), early neural progenitors (eNP) and neural progenitors (NP). Here we demonstrated that sensitivity to PQQ is dependent upon its dose and neural stage of development. Induction of the mitochondrial biogenesis by PQQ at three stages of neural differentiation was evaluated at mtDNA, mRNA and protein level...
May 31, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28572558/synthesis-and-crystal-structure-of-pyrroloquinoline-quinol-pqqh2-and-pyrroloquinoline-quinone-pqq
#15
Kazuto Ikemoto, Shigeki Mori, Kazuo Mukai
Pyrroloquinoline quinone (PQQ) is a water-soluble quinone compound first identified as a cofactor of alcohol- and glucose-dehydrogenases (ADH and GDH) in bacteria. For example, in the process of ADH reaction, alcohol is oxidized to the corresponding aldehyde, and inversely PQQ is reduced to pyrroloquinoline quinol (PQQH2). PQQ and PQQH2 molecules play an important role as a cofactor in ADH and GDH reactions. However, crystal structure analysis has not been performed for PQQ and PQQH2. In the present study, the synthesis of PQQH2 powder crystals was performed under air, by utilizing vitamin C as a reducing agent...
June 1, 2017: Acta Crystallographica Section B, Structural Science, Crystal Engineering and Materials
https://www.readbyqxmd.com/read/28500115/erroneously-elevated-glucose-values-due-to-maltose-interference-in-mutant-glucose-dehydrogenase-pyrroloquinolinequinone-mutant-gdh-pqq-based-glucometer
#16
Partha Pratim Chakraborty, Shinjan Patra, Rana Bhattacharjee, Subhankar Chowdhury
Currently available glucose test strip enzymes include glucose oxidase (GOD) and glucose dehydrogenase (GDH). In GDH-based glucometers, glucose oxidation can be catalysed by different cofactors: nicotinamide adenine dinucleotide (GDH-NAD), flavin adenine dinucleotide (GDH-FAD), pyrroloquinolinequinone (GDH-PQQ) and mutant GDH-PQQ. GOD-based and GDH-NAD-based glucometers are substrate-specific and do not react with sugars other than glucose. GDH-FAD reacts with xylose only in addition to glucose. GDH-PQQ is not glucose-specific; in addition to glucose, it reacts with different other sugars and produces falsely high values of capillary glucose in the presence of such substances...
May 12, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/28484812/metabolic-engineering-of-gluconobacter-oxydans-621h-for-increased-biomass-yield
#17
Ines Kiefler, Stephanie Bringer, Michael Bott
The obligatory aerobic acetic acid bacterium Gluconobacter oxydans incompletely oxidizes carbon sources regio- and stereoselectively in the periplasm and therefore is used industrially for oxidative biotransformations, e. g., in vitamin C production. However, it has a very low biomass yield as the oxidized products largely remain in the medium and cannot be used for anabolism. Cytoplasmic carbon metabolism occurs via the pentose phosphate pathway and the Entner-Doudoroff pathway, whereas glycolysis and the tricarboxylic acid cycle are incomplete...
July 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28481092/nuclear-magnetic-resonance-structure-and-binding-studies-of-pqqd-a-chaperone-required-in-the-biosynthesis-of-the-bacterial-dehydrogenase-cofactor-pyrroloquinoline-quinone
#18
Robert L Evans, John A Latham, Youlin Xia, Judith P Klinman, Carrie M Wilmot
Biosynthesis of the ribosomally synthesized and post-translationally modified peptide (RiPP), pyrroloquinoline quinone (PQQ), is initiated when the precursor peptide, PqqA, is recognized and bound by the RiPP precursor peptide recognition element (RRE), PqqD, for presentation to the first enzyme in the pathway, PqqE. Unlike other RiPP-producing, postribosomal peptide synthesis (PRPS) pathways in which the RRE is a component domain of the first enzyme, PqqD is predominantly a separate scaffolding protein that forms a ternary complex with the precursor peptide and first tailoring enzyme...
May 30, 2017: Biochemistry
https://www.readbyqxmd.com/read/28469179/highly-selective-and-sensitive-self-powered-glucose-sensor-based-on-capacitor-circuit
#19
Gymama Slaughter, Tanmay Kulkarni
Enzymatic glucose biosensors are being developed to incorporate nanoscale materials with the biological recognition elements to assist in the rapid and sensitive detection of glucose. Here we present a highly sensitive and selective glucose sensor based on capacitor circuit that is capable of selectively sensing glucose while simultaneously powering a small microelectronic device. Multi-walled carbon nanotubes (MWCNTs) is chemically modified with pyrroloquinoline quinone glucose dehydrogenase (PQQ-GDH) and bilirubin oxidase (BOD) at anode and cathode, respectively, in the biofuel cell arrangement...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28454714/growth-promotion-of-peanut-arachis-hypogaea-l-and-maize-zea-mays-l-plants-by-single-and-mixed-cultures-of-efficient-phosphate-solubilizing-bacteria-that-are-tolerant-to-abiotic-stress-and-pesticides
#20
María Soledad Anzuay, María Gabriela Ruiz Ciancio, Liliana Mercedes Ludueña, Jorge Guillermo Angelini, Germán Barros, Nicolás Pastor, Tania Taurian
The aims of this study were, to analyze in vitro phosphate solubilization activity of six native peanut bacteria and to determine the effect of single and mixed inoculation of these bacteria on peanut and maize plants. Ability to produce organic acids and cofactor PQQ, to solubilize FePO4 and AlPO4 and phosphatase activity were analyzed. Also, the ability to solubilize phosphate under abiotic stress and in the presence of pesticides of the selected bacteria was determined. The effect of single and mixed bacterial inocula was analyzed on seed germination, maize plant growth and in a crop rotation plant assay with peanut and maize...
June 2017: Microbiological Research
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