keyword
MENU ▼
Read by QxMD icon Read
search

Minor antigen

keyword
https://www.readbyqxmd.com/read/28104399/structural-basis-for-the-regulation-of-nuclear-import-of-epstein-barr-virus-nuclear-antigen-1-ebna1-by-phosphorylation-of-the-nuclear-localization-signal
#1
Ryohei Nakada, Hidemi Hirano, Yoshiyuki Matsuura
Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is expressed in every EBV-positive tumor and is essential for the maintenance, replication, and transcription of the EBV genome in the nucleus of host cells. EBNA1 is a serine phosphoprotein, and it has been shown that phosphorylation of S385 in the nuclear localization signal (NLS) of EBNA1 increases the binding affinity to the nuclear import adaptor importin-α1 as well as importin-α5, and stimulates nuclear import of EBNA1. To gain insights into how phosphorylation of the EBNA1 NLS regulates nuclear import, we have determined the crystal structures of two peptide complexes of importin-α1: one with S385-phosphorylated EBNA1 NLS peptide, determined at 2...
January 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28102730/alloimmunisation-in-transfused-patients-serial-cross-matching-in-a-population-of-hospitalised-cats
#2
Layla Hourani, Christiane Weingart, Barbara Kohn
Objectives Cross-matching is currently recommended as part of pre-transfusion testing for repeat transfusions in cats 4 days after having received an initial transfusion. This prospective study determined when and if cats developed positive cross-match (CM) results after having been transfused with AB-compatible blood. Methods Donors were selected according to standard transfusion safety protocols. Twenty-one hospitalised anaemic recipients (blood type A: n = 20; blood type B: n = 1) received 1-4 (median 2) whole-blood transfusions (WBTs) over 1-6 days (median 2) in 33 transfusion instances...
January 1, 2017: Journal of Feline Medicine and Surgery
https://www.readbyqxmd.com/read/28074593/microtubule-associated-protein-map-1b-novel-paraneoplastic-biomarker-running-head-map-1b-igg-novel-paraneoplastic-biomarker
#3
Avi Gadoth, Thomas J Kryzer, Jim Fryer, Andrew McKeon, Vanda A Lennon, Sean J Pittock
To report the identification of microtubule associated protein (MAP)1B as the antigen of the previously described PCA-2 antibody, its frequency and clinical, oncological and serological associations. Methods Archival serum or cerebrospinal fluid (CSF) specimens were available from 96 of 118 consecutive PCA-2-IgG-seropositive patients identified 1993-2016. The autoantigen, defined in mouse brain lysate by western blot and mass spectrometry, was confirmed by dual immunohistochemical staining using commercial antibodies...
January 11, 2017: Annals of Neurology
https://www.readbyqxmd.com/read/28073635/pcna-dependent-cellular-activities-tolerate-dramatic-perturbations-in-pcna-client-interactions
#4
Rosemary H C Wilson, Antonio J Biasutto, Lihao Wang, Roman Fischer, Emma L Baple, Andrew H Crosby, Erika J Mancini, Catherine M Green
Proliferating cell nuclear antigen (PCNA) is an essential cofactor for DNA replication and repair, recruiting multiple proteins to their sites of action. We examined the effects of the PCNA(S228I) mutation that causes PCNA-associated DNA repair disorder (PARD). Cells from individuals affected by PARD are sensitive to the PCNA inhibitors T3 and T2AA, showing that the S228I mutation has consequences for undamaged cells. Analysis of the binding between PCNA and PCNA-interacting proteins (PIPs) shows that the S228I change dramatically impairs the majority of these interactions, including that of Cdt1, DNMT1, PolD3(p66) and PolD4(p12)...
December 31, 2016: DNA Repair
https://www.readbyqxmd.com/read/28067665/selective-graft-versus-leukemia-depends-on-magnitude-and-diversity-of-the-alloreactive-t-cell-response
#5
Cornelis A M van Bergen, Simone A P van Luxemburg-Heijs, Liesbeth C de Wreede, Matthijs Eefting, Peter A von dem Borne, Peter van Balen, Mirjam H M Heemskerk, Arend Mulder, Fransiscus H J Claas, Marcelo A Navarrete, Wilhelmina M Honders, Caroline E Rutten, Hendrik Veelken, Inge Jedema, Constantijn J M Halkes, Marieke Griffioen, J H Frederik Falkenburg
Patients with leukemia who receive a T cell-depleted allogeneic stem cell graft followed by postponed donor lymphocyte infusion (DLI) can experience graft-versus-leukemia (GVL) reactivity, with a lower risk of graft-versus-host disease (GVHD). Here, we have investigated the magnitude, diversity, and specificity of alloreactive CD8 T cells in patients who developed GVL reactivity after DLI in the absence or presence of GVHD. We observed a lower magnitude and diversity of CD8 T cells for minor histocompatibility antigens (MiHAs) in patients with selective GVL reactivity without GVHD...
January 9, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28062697/subclinical-herpes-simplex-virus-type-1-infections-provide-site-specific-resistance-to-an-unrelated-pathogen
#6
Alexander M Rowe, Hongming Yun, Benjamin R Treat, Paul R Kinchington, Robert L Hendricks
HSV-1 infections of the cornea range in severity from minor transient discomfort to the blinding disease herpes stromal keratitis, yet most patients experience a single episode of epithelial keratitis followed by re-establishment of a clear cornea. We asked whether a single transient episode of HSV-1 epithelial keratitis causes long-term changes in the corneal microenvironment that influence immune responses to subsequent corneal infection or trauma. We showed that C57BL/6 mouse corneas infected with HSV-1 KOS, which induces transient herpes epithelial keratitis without herpes stromal keratitis sequelae, possessed a significant leukocytic infiltrate composed primarily of CD4(+) T cells and macrophages along with elevated chemokines and cytokines that persisted without loss of corneal clarity (subclinical inflammation)...
January 6, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28061542/an-efficient-method-for-native-protein-purification-in-the-selected-range-from-prostate-cancer-tissue-digests
#7
Rumana Ahmad, Carrie D Nicora, Anil K Shukla, Richard D Smith, Wei-Jun Qian, Alvin Y Liu
BACKGROUND: Prostate cancer (CP) cells differ from their normal counterpart in gene expression. Genes encoding secreted or extracellular proteins with increased expression in CP may serve as potential biomarkers. For their detection and quantification, assays based on monoclonal antibodies are best suited for development in the clinical setting. One approach to obtain antibodies is to use recombinant proteins as immunogen. However, the synthesis of recombinant protein for each identified candidate is time-consuming and expensive...
December 2016: Chinese Clinical Oncology
https://www.readbyqxmd.com/read/28043513/modulation-of-cd4-and-cd8-response-to-quantiferon-tb-plus-in-patients-with-active-tuberculosis-and-latent-tuberculosis-infection-followed-over-time-during-treatment
#8
Teresa Chiacchio, Elisa Petruccioli, Ilaria Pepponi, Valentina Vanini, Gina Gualano, Daniela Cirillo, Fabrizio Palmieri, Giuseppe Ippolito, Delia Goletti
OBJECTIVE/BACKGROUND: Interferon (IFN)-γ release assays (IGRA) are designed for diagnosing tuberculosis (TB) infection. The new IGRA, QuantiFERON-TB Plus (QFT-Plus), is based on the enzyme-linked immunosorbent assay detection of IFN-γ after stimulation with Mycobacterium tuberculosis TB1 and TB2 antigens. TB1 elicits a cellular-mediated immune (CMI) response by CD4 T cells, and TB2 contains peptides recognized by both CD4 and CD8 T cells. The aim of the study is to characterize the CMI to QFT-Plus peptides in active TB and latent TB infection (LTBI) at baseline and during or after specific treatment (follow-up)...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28039303/antigenic-determinants-of-der-p-1-specificity-and-cross-reactivity-associated-with-ige-antibody-recognition
#9
Jill Glesner, Lisa D Vailes, Caleb Schlachter, Nicholas Mank, Wladek Minor, Tomasz Osinski, Maksymilian Chruszcz, Martin D Chapman, Anna Pomés
Der p 1 and Der f 1 are major allergens from Dermatophagoides pteronyssinus and D. farinae, respectively. An analysis of antigenic determinants on both allergens was performed by site-directed mutagenesis. The analysis was based on the x-ray crystal structures of the allergens in complex with Fab fragments of three murine mAbs that interfere with IgE Ab binding: the two Der p 1-specific mAbs 5H8 and 10B9, and the cross-reactive mAb 4C1. On one hand, selected residues in the epitopes for mAb 5H8 and mAb 4C1 were substituted with amino acids that resulted in impaired Ab binding to Der p 1...
December 30, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28034917/active-surveillance-for-low-risk-localized-prostate-cancer-what-do-men-and-their-partners-think
#10
Arun Mallapareddi, Julie Ruterbusch, Elyse Reamer, Susan Eggly, Jinping Xu
BACKGROUND: Active surveillance (AS) is recognized as a reasonable treatment option for low-risk localized prostate cancer (LPC) but continues to be chosen by a minority of men. To date, limited data are available regarding reasons why men with low-risk LPC adopt AS. PURPOSE: The aim of this study is to better understand conceptualizations, experiences and reasons why men with low-risk LPC and their partners adopt AS. METHODS: We conducted five focus groups (FGs), three among men with low-risk LPC who had chosen AS and two with their partners...
December 29, 2016: Family Practice
https://www.readbyqxmd.com/read/28017213/revised-diagnostic-criteria-for-neurocysticercosis
#11
REVIEW
O H Del Brutto, T E Nash, A C White, V Rajshekhar, P P Wilkins, G Singh, C M Vasquez, P Salgado, R H Gilman, H H Garcia
BACKGROUND: A unified set of criteria for neurocysticercosis (NCC) has helped to standardize its diagnosis in different settings. METHODS: Cysticercosis experts were convened to update current diagnostic criteria for NCC according to two principles: neuroimaging studies are essential for diagnosis, and all other information provides indirect evidence favoring the diagnosis. Recent diagnostic advances were incorporated to this revised set. RESULTS: This revised set is structured in absolute, neuroimaging and clinical/exposure criteria...
January 15, 2017: Journal of the Neurological Sciences
https://www.readbyqxmd.com/read/28002060/embryonic-origin-of-primary-colon-cancer-predicts-pathologic-response-and-survival-in-patients-undergoing-resection-for-colon-cancer-liver-metastases
#12
Suguru Yamashita, Kristoffer Watten Brudvik, Scott E Kopetz, Dipen Maru, Callisia N Clarke, Guillaume Passot, Claudius Conrad, Yun Shin Chun, Thomas A Aloia, Jean-Nicolas Vauthey
BACKGROUND: The aim of this study was to determine the prognostic value of embryonic origin in patients undergoing resection after chemotherapy for colon cancer liver metastases (CCLM). METHODS: We identified 725 patients with primary colon cancer and known RAS mutation status who underwent hepatic resection after preoperative chemotherapy for CCLM (1990 to 2015). Survival after resection of CCLM from midgut origin (n = 238) and hindgut origin (n = 487) was analyzed...
December 19, 2016: Annals of Surgery
https://www.readbyqxmd.com/read/27996105/a-cluster-of-group-a-streptococcal-infections-in-a-skilled-nursing-facility-the-potential-role-of-healthcare-worker-presenteeism
#13
Miwako Kobayashi, Meghan M Lyman, Louise K Francois Watkins, Karrie-Ann Toews, Leon Bullard, Rachel A Radcliffe, Bernard Beall, Gayle Langley, Chris Van Beneden, Nimalie D Stone
OBJECTIVES: To determine the extent of a group A streptococcus (GAS) cluster (2 residents with invasive GAS (invasive case-patients), 2 carriers) caused by a single strain (T antigen type 2 and M protein gene subtype 2.0 (T2, emm 2.0)), evaluate factors contributing to transmission, and provide recommendations for disease control. DESIGN: Cross-sectional analysis and retrospective review. SETTING: Skilled nursing facility (SNF). PARTICIPANTS: SNF residents and staff...
December 2016: Journal of the American Geriatrics Society
https://www.readbyqxmd.com/read/27992776/differential-response-of-olfactory-sensory-neuron-populations-to-copper-ion-exposure-in-zebrafish
#14
Maurizio Lazzari, Simone Bettini, Liliana Milani, Maria Gabriella Maurizii, Valeria Franceschini
The peripheral olfactory system of fish is in direct contact with the external aqueous environment, so dissolved contaminants can easily impair sensory functions and cause neurobehavioral injuries. The olfactory epithelium of fish is arranged in lamellae forming a rosette in the olfactory cavity and contains three main types of olfactory sensory neurons (OSNs): ciliated (cOSNs) and microvillous olfactory sensory neurons (mOSNs), common to all vertebrates, and a third minor group of olfactory neurons, crypt cells, absent in tetrapods...
December 14, 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27982091/a-detergent-based-procedure-for-the-preparation-of-igg-like-bispecific-antibodies-in-high-yield
#15
Jyoti Gupta, Mehboob Hoque, Masihuz Zaman, Rizwan Hasan Khan, M Saleemuddin
Bispecific antibodies (BsAbs), with the ability to recognize two different epitopes simultaneously, offer remarkable advantages in bioassays, cancer therapy, biosensors, and enzyme electrodes. Preparation and purification of BsAbs in adequate quantities remains a major hurdle in their use in various applications. Poor yield is also the principal limitation in the preparation of BsAbs by the redox procedure. IgG with reduced inter-heavy chain disulfides do not dissociate into half molecules at neutral pH. In this study, we report that the dissociation occurs in presence of sodium dodecyl sulphate (SDS) and inclusion of the detergent during the redox procedure results in remarkable increase in the formation of the BsAbs...
December 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27981498/humoral-immunity-profiling-of-subjects-with-myalgic-encephalomyelitis-using-a-random-peptide-microarray-differentiates-cases-from-controls-with-high-specificity-and-sensitivity
#16
Sahajpreet Singh, Phillip Stafford, Karen A Schlauch, Richard R Tillett, Martin Gollery, Stephen Albert Johnston, Svetlana F Khaiboullina, Kenny L De Meirleir, Shanti Rawat, Tatjana Mijatovic, Krishnamurthy Subramanian, András Palotás, Vincent C Lombardi
Myalgic encephalomyelitis (ME) is a complex, heterogeneous illness of unknown etiology. The search for biomarkers that can delineate cases from controls is one of the most active areas of ME research; however, little progress has been made in achieving this goal. In contrast to identifying biomarkers that are directly involved in the pathological process, an immunosignature identifies antibodies raised to proteins expressed during, and potentially involved in, the pathological process. Although these proteins might be unknown, it is possible to detect antibodies that react to these proteins using random peptide arrays...
December 15, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27927927/use-of-recombinant-antigens-for-sensitive-serodiagnosis-of-american-tegumentary-leishmaniasis-caused-by-different-leishmania-species
#17
Camila Massae Sato, Maria Carmen Arroyo Sanchez, Beatriz Julieta Celeste, Malcolm S Duthie, Jeffrey Guderian, Steven G Reed, Maria Edileuza Felinto Brito, Marliane Batista Campos, Helia Valeria de Souza Encarnação, Jorge Guerra, Tirza Gabrielle Ramos de Mesquita, Suzana Kanawati Pinheiro, Rajendranath Ramasawmy, Fernando Tobias Silveira, Marina de Assis Souza, Hiro Goto
American tegumentary leishmaniasis (ATL; also known as cutaneous leishmaniasis (CL)) is caused by various species of protozoa of the genus Leishmania The diagnosis is achieved on a clinical, epidemiological and pathological basis supported with positive parasitological exams and demonstration of Leishmanin delayed-type hypersensitivity. Serological assays are not routinely used in the diagnosis because many are considered to have low sensitivity and the particular Leishmania species causing the disease can lead to variable perfomance...
December 7, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27895917/validation-of-biomarkers-to-predict-response-to-immunotherapy-in-cancer-volume-i-pre-analytical-and-analytical-validation
#18
REVIEW
Giuseppe V Masucci, Alessandra Cesano, Rachael Hawtin, Sylvia Janetzki, Jenny Zhang, Ilan Kirsch, Kevin K Dobbin, John Alvarez, Paul B Robbins, Senthamil R Selvan, Howard Z Streicher, Lisa H Butterfield, Magdalena Thurin
Immunotherapies have emerged as one of the most promising approaches to treat patients with cancer. Recently, there have been many clinical successes using checkpoint receptor blockade, including T cell inhibitory receptors such as cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) and programmed cell death-1 (PD-1). Despite demonstrated successes in a variety of malignancies, responses only typically occur in a minority of patients in any given histology. Additionally, treatment is associated with inflammatory toxicity and high cost...
2016: Journal for Immunotherapy of Cancer
https://www.readbyqxmd.com/read/27886076/pathogens-inactivated-by-low-energy-electron-irradiation-maintain-antigenic-properties-and-induce-protective-immune-responses
#19
Jasmin Fertey, Lea Bayer, Thomas Grunwald, Alexandra Pohl, Jana Beckmann, Gaby Gotzmann, Javier Portillo Casado, Jessy Schönfelder, Frank-Holm Rögner, Christiane Wetzel, Martin Thoma, Susanne M Bailer, Ekkehard Hiller, Steffen Rupp, Sebastian Ulbert
Inactivated vaccines are commonly produced by incubating pathogens with chemicals such as formaldehyde or β-propiolactone. This is a time-consuming process, the inactivation efficiency displays high variability and extensive downstream procedures are often required. Moreover, application of chemicals alters the antigenic components of the viruses or bacteria, resulting in reduced antibody specificity and therefore stimulation of a less effective immune response. An alternative method for inactivation of pathogens is ionizing radiation...
November 23, 2016: Viruses
https://www.readbyqxmd.com/read/27872868/minor-antigen-disparities-impede-induction-of-long-lasting-chimerism-and-tolerance-through-bone-marrow-transplantation-with-costimulation-blockade
#20
Sinda Bigenzahn, Ines Pree, Christoph Klaus, Nina Pilat, Benedikt Mahr, Elisabeth Schwaiger, Patrick Nierlich, Friedrich Wrba, Thomas Wekerle
Mixed chimerism and tolerance can be successfully induced in rodents through allogeneic bone marrow transplantation (BMT) with costimulation blockade (CB), but varying success rates have been reported with distinct models and protocols. We therefore investigated the impact of minor antigen disparities on the induction of mixed chimerism and tolerance. C57BL/6 (H2(b)) mice received nonmyeloablative total body irradiation (3 Gy), costimulation blockade (anti-CD40L mAb and CTLA4Ig), and 2 × 10(7) bone marrow cells (BMC) from either of three donor strains: Balb/c (H2(d)) (MHC plus multiple minor histocompatibility antigen (mHAg) mismatched), B10...
2016: Journal of Immunology Research
keyword
keyword
49407
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"