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Cristina García, Clara Marín, Pablo Catalá-Gregori, Jose Miguel Soriano
INTRODUCTION: Salmonellosis is a highly prevalent disease still searching for preventive tools to avoid contamination level priority public health. OBJECTIVE: The in vitro effect of bacteriophages against Salmonella enteritidis was evaluated as a prevention tool. METHOD: Two tests with three concentrations of bacteriophages were conducted against two strains of Salmonella Enteritidis inoculated in fresh faeces of laying hens. Each test had a positive control...
June 1, 2015: Nutrición Hospitalaria: Organo Oficial de la Sociedad Española de Nutrición Parenteral y Enteral
Chaker Tlili, Esen Sokullu, Mohammadali Safavieh, Mona Tolba, Minhaz Uddin Ahmed, Mohammed Zourob
Here, we integrate two complementary detection strategies for the identification and quantification of Escherichia coli based on bacteriophage T4 as a natural bioreceptor for living bacteria cells. The first approach involves screening and viability assays, employing bacteriophage as the recognition element in label-free electrochemical impedance spectroscopy. The complementary approach is a confirmation by loop-mediated isothermal amplification (LAMP) to amplify specifically the E. coli Tuf gene after lysis of the bound E...
May 21, 2013: Analytical Chemistry
Fuke Wang, Binrui Cao, Chuanbin Mao
Inorganic ions may direct the self-assembly of biomacromolecules into nanostructures which can further be used as a reactant and matrix for nanomaterials synthesis and assembly. Here we use bone mineral and filamentous bacteriophage as a model to demonstrate this concept. Divalent calcium ions are found to trigger the electrostatic self-assembly of anionic nanofiber-like bacteriophages into bundle structures where calcium ions are pre-organized between bacteriophage nanofibers. The resultant Ca(2+)-bacteriophage bundles can be separated and purified from the aqueous solution...
June 22, 2010: Chemistry of Materials: a Publication of the American Chemical Society
Jonathan O'Driscoll, Daniel F Heiter, Geoffrey G Wilson, Gerald F Fitzgerald, Richard Roberts, Douwe van Sinderen
BACKGROUND: Restriction/modification systems provide the dual function of protecting host DNA against restriction by methylation of appropriate bases within their recognition sequences, and restriction of foreign invading un-methylated DNA, such as promiscuous plasmids or infecting bacteriphage. The plasmid-encoded LlaJI restriction/modification system from Lactococcus lactis recognizes an asymmetric, complementary DNA sequence, consisting of 5'GACGC'3 in one strand and 5'GCGTC'3 in the other and provides a prodigious barrier to bacteriophage infection...
2006: BMC Microbiology
Vadim V Mesyanzhinov, Johan Robben, Barbara Grymonprez, Victor A Kostyuchenko, Maria V Bourkaltseva, Nina N Sykilinda, Victor N Krylov, Guido Volckaert
Bacteriophage phiKZ is a giant virus that efficiently infects Pseudomonas aeruginosa strains pathogenic to human and, therefore, it is attractive for phage therapy. We present here the complete phiKZ genome sequence and a preliminary analysis of its genome structure. The 280,334 bp genome is a linear, circularly permutated and terminally redundant, A+T-rich double-stranded DNA molecule. The phiKZ DNA has no detectable sequence homology to other viruses and microorganisms, and it does not contain NotI, PstI, SacI, SmaI, XhoI, and XmaIII endonuclease restriction sites...
March 15, 2002: Journal of Molecular Biology
B F Cheetham, D B Tattersall, G A Bloomfield, J I Rood, M E Katz
Dichelobacter nodosus is the principal causative agent of ovine footrot. Nucleotide (nt) sequences from the D. nodosus genome have been isolated and a series of overlapping lambda clones defining vap (virulence-associated protein) regions 1, 2 and 3 have been reported [Katz et al., J. Bacteriol. 176 (1994) 2663-2669]. In the present study, the limits of the virulence-associated (va) DNA around vap regions 1 and 3 were determined by dot-blot hybridization experiments using plasmid subclones to probe genomic DNA from the D...
August 30, 1995: Gene
M Rhoades, J Schwartz, J M Wahl
Deletion mutants of bacteriphage T5 can be identified by analyzing the DNA content of single plaques, after alkaline denaturation, by agarose gel electrophoresis. Use of this procedure has resulted in the isolation of a new mutant, designated del2, that lacks a 1.6% segment located 68 to 70% from the left end of T5 DNA. This segment occurs between the early and late gene regions of T5 DNA.
November 1980: Journal of Virology
J P Claverys, J C Lefevre, A M Sicard
The genetic fate of a fragment of Streptococcus pneumoniae DNA cloned into a derivative of the Escherichia coli bacteriphage lambda has been studied in pneumococcal transformation. Transforming activity of this hybrid DNA is 8 times higher than standard S. pneumoniae DNA. Hybrid DNA is mutagenic for the recipient bacteria. Mutations are induced at a rate of 2 per 1000 transformation events. Most of these mutations are deletions adjacent to the cloned pneumococcal fragment, starting at or near its extremities and extending outside...
June 1980: Proceedings of the National Academy of Sciences of the United States of America
J A Steitz
No abstract text is available yet for this article.
May 14, 1968: Journal of Molecular Biology
C L Parsons, R E Beardsley
No abstract text is available yet for this article.
June 1968: Journal of Virology
L P Gage, E P Geiduschek
No abstract text is available yet for this article.
April 1971: Virology
D Bray, P W Robbins
No abstract text is available yet for this article.
December 28, 1967: Journal of Molecular Biology
P H Pouwels, C M Knijnenburg, J van Rotterdam, J A Cohen
No abstract text is available yet for this article.
March 14, 1968: Journal of Molecular Biology
M Osborn, S Person
No abstract text is available yet for this article.
July 1967: Mutation Research
J Huppert, L Blum-Emerique, M M Breugnon
No abstract text is available yet for this article.
October 1967: Virology
W C Schmidt, C D Jeffries
A bacteriphage typing scheme for differentiating Proteus isolated from clinical specimens was developed. Twenty-one distinct patterns of lysis were seen when 15 bacteriophages isolated on 8 Proteus mirabilis, 1 P. vulgaris, and 1 P. morganii were used to type 162 of 189 (85.7%) P. mirabilis and P. vulgaris isolates. Seven phages isolated on 3 P. morganii were used to type 13 of 19 (68.4%) P. morganii isolates. Overall, 84.1% of the 208 isolates were lysed by at least 1 phage at routine test dilution (RTD) or 1,000 x RTD...
January 1974: Applied Microbiology
A Ia Veselov
No abstract text is available yet for this article.
February 1969: Antibiotiki
C Shearman, H Underwood, K Jury, M Gasson
A gene for the lysin of Lactococcus lactis bacteriphage phi vML3 was cloned using an Escherichia coli/bacteriophage lambda host-vector system. The gene was detected by its expression of antimicrobial activity against L. lactis cells in a bioassay. The cloned fragment was analysed by sub-cloning on to E. coli plasmid vectors and by restriction endonuclease and deletion mapping. Its entire DNA sequence was determined and an open reading frame for the lysin structural gene was identified. The sequenced lysin gene would express a protein of 187 amino acids with a molecular weight of 21,090, which is in good agreement with that of a protein detected after in vitro transcription and translation of DNA encoding the gene...
August 1989: Molecular & General Genetics: MGG
J V Paietta, T L McDonald, A H Doermann
An efficient procedure is described for isolating bacteriophage T4 mutants that produce particles with giant capsids. The selective parameters of the technique rely primarily on the giants' characteristic density in cesium gradients and on their resistance to UV radiation.
May 1976: Journal of Virology
C A Pachl, E T Young
Bacteriphage T7 RNAs have been fractionated on preparative polyacrylamide gels. The in vitro coding capacities of the RNAs have been determined by translation of the RNAs in a cell-free system and analysis of the polypeptide products on sodium dodecyl sulfate polyacrylamide slab gels. The T7 early RNAs are fractionated according to their molecular weight and without intermolecular aggregation. Fractionation of the late T7 RNAs gives rise to 10 major RNAs, ranging in size from 0.29 X 10(6) daltons to 2.05 X 10(6) daltons...
February 1976: Proceedings of the National Academy of Sciences of the United States of America
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