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Sialic Acid

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https://www.readbyqxmd.com/read/28651174/label-free-chronopotentiometric-glycoprofiling-of-prostate-specific-antigen-using-sialic-acid-recognizing-lectins
#1
Stefan Belicky, Hana Černocká, Tomas Bertok, Alena Holazova, Kamila Réblová, Emil Paleček, Jan Tkac, Veronika Ostatná
In recent decades, it has become clear that most of human proteins are glycosylated and that protein glycosylation plays an important role in health and diseases. At present, simple, fast and inexpensive methods are sought for clinical applications and particularly for improved diagnostics of various diseases, including cancer. We propose a label- and reagent-free electrochemical method based on chronopotentiometric stripping (CPS) analysis and a hanging mercury drop electrode for the detection of interaction of sialylated protein biomarker a prostate specific antigen (PSA) with two important lectins: Sambucus nigra agglutinin (SNA) and Maackia amurensis agglutinin (MAA)...
June 19, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/28649697/bioorthogonal-labeling-of-human-prostate-cancer-tissue-slice-cultures-for-glycoproteomics
#2
David R Spiciarich, Rosalie Nolley, Sophia L Maund, Sean C Purcell, Jason Herschel, Anthony T Iavarone, Donna M Peehl, Carolyn R Bertozzi
Sialylated glycans are found at elevated levels in many types of cancer and have been implicated in disease progression. However, the specific glycoproteins that contribute to the cancer cell-surface sialylation are not well characterized, specifically in bona fide human disease tissue. Metabolic and bioorthogonal labeling methods have previously enabled the enrichment and identification of sialoglycoproteins from cultured cells and model organisms. Herein, we report the first application of this glycoproteomic platform to human tissues cultured ex vivo...
June 26, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28643465/severe-hemolysis-after-plasma-transfusion-in-a-neonate-with-necrotizing-enterocolitis-clostridium-perfringens-infection-and-red-blood-cell-t-polyagglutination
#3
Julia Moh-Klaren, Gwellaouen Bodivit, Myriam Jugie, Philippe Chadebech, Laurent Chevret, Mostafa Mokhtari, Xavier Chamillard, Philippe Gallon, Pierre Tissières, Philippe Bierling, Rachid Djoudi, France Pirenne, Nicolas Burin-des-Roziers
BACKGROUND: Red blood cell (RBC) Thomsen-Friedenreich antigen exposure (T activation) in infants with necrotizing enterocolitis (NEC) has occasionally been associated with posttransfusional intravascular hemolysis thought to be due to anti-T antibodies in the donor plasma. STUDY DESIGN AND METHODS: We describe an infant with NEC and Clostridium perfringens infection complicated by severe hemolysis after plasma transfusion. After this case, infants with confirmed NEC were prospectively evaluated for T activation...
June 22, 2017: Transfusion
https://www.readbyqxmd.com/read/28641925/safety-pharmacokinetics-and-sialic-acid-production-after-oral-administration-of-n-acetylmannosamine-mannac-to-subjects-with-gne-myopathy
#4
Xin Xu, Amy Q Wang, Lea L Latham, Frank Celeste, Carla Ciccone, May Christine Malicdan, Barry Goldspiel, Pramod Terse, James Cradock, Nora Yang, Selwyn Yorke, John C McKew, William A Gahl, Marjan Huizing, Nuria Carrillo
GNE myopathy is a rare, autosomal recessive, inborn error of sialic acid metabolism, caused by mutations in GNE, the gene encoding UDP-N-acetyl-glucosamine-2-epimerase/N-acetylmannosamine kinase. The disease manifests as an adult-onset myopathy characterized by progressive skeletal muscle weakness and atrophy. There is no medical therapy available for this debilitating disease. Hyposialylation of muscle glycoproteins likely contributes to the pathophysiology of this disease. N-acetyl-D-mannosamine (ManNAc), an uncharged monosaccharide and the first committed precursor in the sialic acid biosynthetic pathway, is a therapeutic candidate that prevents muscle weakness in the mouse model of GNE myopathy...
April 26, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28640212/synthesis-and-in-vitro-anti-influenza-virus-evaluation-of-novel-sialic-acid-c-5-and-c-9-pentacyclic-triterpene-derivatives
#5
Xu Han, Long-Long Si, Yong-Ying Shi, Zi-Bo Fan, Shou-Xin Wang, Zhen-Yu Tian, Man Li, Jia-Qi Sun, Ping-Xuan Jiao, Fu-Xiang Ran, Yong-Min Zhang, De-Min Zhou, Su-Long Xiao
The emergence of drug resistant variants of the influenza virus has led to a great need to identify novel and effective antiviral agents. In our previous study, a series of sialic acid (C-2 and C-4)-pentacyclic triterpene conjugates have been synthesized, and a five-fold more potent antiviral activity was observed when sialic acid was conjugated with pentacyclic triterpene via C-4 than C-2. It was here that we further reported the synthesis and anti-influenza activity of novel sialic acid (C-5 and C-9)-pentacyclic triterpene conjugates...
June 22, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28638072/diet-induced-reconstruction-of-mucosal-microbiota-associated-with-alterations-of-epithelium-lectin-expression-and-regulation-in-the-maintenance-of-rumen-homeostasis
#6
Hong Shen, Zhongyan Lu, Zhihui Xu, Zanming Shen
It is unknown whether lectins of the rumen epithelium contribute to the recognition of mucosal microbes and activation of tolerogenic cytokines in ruminant animals. We applied an integrated method of RNA-seq and 16S rRNA gene sequencing to investigate alterations of epithelial lectin expression and regulation with a diet-induced reconstruction of the mucosal microbiota in the goat rumen. Our results showed that the diversity and richness of the rumen mucosal microbiota were promoted by the dietary concentrate...
June 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28635265/metabolic-oligosaccharide-engineering-with-alkyne-sialic-acids-confers-neuraminidase-resistance-and-inhibits-influenza-reproduction
#7
Torben Heise, Christian Büll, Daniëlle M H Beurskens, Emiel Rossing, Marien de Jonge, Gosse J Adema, Thomas J Boltje, Jeroen Langereis
Metabolic incorporation of azide or alkyne modified sialic acids into the cellular glycosylation pathway enables the study of sialoglycan expression, localization and trafficking via bioorthogonal chemistry. Herein we report that such modifications of the sialic acid sugar can have a profound influence on their hydrolysis by neuraminidases (sialidase). Azidoacetyl modified sialic acids were prone to neuraminidase cleavage, whereas propargyloxycarbonyl (Poc) modified sialic acids were largely resistent to cleavage...
June 21, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28634480/fc-galactosylation-of-human-immunoglobulin-gamma-isotypes-improves-c1q-binding-and-enhances-complement-dependent-cytotoxicity
#8
Benjamin Peschke, Christian W Keller, Patrick Weber, Isaak Quast, Jan D Lünemann
Binding of the complement component C1q to the CH2 domain of antigen-bound immunoglobulin gamma (IgG) activates the classical complement pathway and depends on its close proximity to Fc fragments of neighboring antibodies. IgG subclasses contain a highly conserved asparagine 297 (N)-linked biantennary glycan within their CH2 domains, the core structure of which can be extended with terminal galactose and sialic acid residues. To investigate whether Fc-glycosylation regulates effector functions of human IgG subclasses, we cloned the antigen-binding region of the CD20-specific monoclonal antibody rituximab into IgG isotype expression vectors...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28633281/the-sweet-side-of-the-pathogenic-neisseria-the-role-of-glycan-interactions-in-colonisation-and-disease
#9
Tsitsi D Mubaiwa, Evgeny A Semchenko, Lauren E Hartley-Tassell, Christopher J Day, Michael P Jennings, Kate L Seib
Glycomics is a rapidly growing field that focuses on the structure and function of carbohydrates (glycans) in biological systems. Glycan interactions play a major role in infectious disease, at all stages of colonisation and disease progression. Neisseria meningitidis, the cause of meningococcal sepsis and meningitis, and Neisseria gonorrhoeae, which causes the sexually transmitted infection gonorrhoea, are responsible for significant morbidity and mortality worldwide. N. meningitidis displays a range of surface glycosylations including capsule polysaccharide, lipooligosaccharide and O-linked glycoproteins...
June 13, 2017: Pathogens and Disease
https://www.readbyqxmd.com/read/28629745/exposure-to-non-microbial-n-glycolylneuraminic-acid-protects-farmers-children-against-airway-inflammation-and-colitis
#10
Remo Frei, Ruth Ferstl, Caroline Roduit, Mario Ziegler Dipl-Ing, Elisa Schiavi, Weronika Barcik, Noelia Rodriguez-Perez, Oliver F Wirz, Marcin Wawrzyniak, Benoit Pugin, Dirk Nehrbass, Marek Jutel, Sylwia Smolinska, Patrycja Konieczna, Christian Bieli, Susanne Loeliger, Marco Waser, Göran Pershagen, Josef Riedler, Martin Depner, Bianca Schaub, Jon Genuneit, Harald Renz, Juha Pekkanen, Anne M Karvonen, Jean-Charles Dalphin, Marianne van Hage, Gert Doekes, Mübeccel Akdis, Charlotte Braun-Fahrländer, Cezmi A Akdis, Erika von Mutius, Liam O'Mahony, Roger P Lauener
BACKGROUND: Childhood exposure to a farm environment has been shown to protect against the development of inflammatory diseases such as allergy, asthma, and inflammatory bowel disease. OBJECTIVE: We sought to investigate whether besides exposure to microbes also exposure to structures of non-microbial origin such as the sialic acid N-Glycolylneuraminic acid (Neu5Gc) may play a significant role. METHODS: Exposure to Neu5Gc was evaluated by quantifying anti-Neu5Gc antibody levels in the sera of children enrolled in two farm studies: the PARSIFAL study (n=299) and the PASTURE birth cohort (cord blood (n=836), 1 year (n=734), 4...
June 16, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28624473/antibody-blood-brain-barrier-efflux-is-modulated-by-glycan-modification
#11
John M Finke, Kari R Ayres, Ryan P Brisbin, Hali A Hill, Emily E Wing, William A Banks
BACKGROUND: Drug delivery to the brain is a major roadblock to treatment of Alzheimer's disease. Recent results of the PRIME study indicate that increasing brain penetration of antibody drugs improves Alzheimer's treatment outcomes. New approaches are needed to better accomplish this goal. Based on prior evidence, the hypothesis that glycan modification alters antibody blood-brain barrier permeability was tested here. METHODS: The blood-brain barrier permeability coefficient Pe of different glycosylated states of anti-amyloid IgG was measured using in vitro models of brain microvascular endothelial cells...
June 14, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28623326/sialic-acid-linkage-differentiation-of-glycopeptides-using-capillary-electrophoresis-electrospray-ionization-mass-spectrometry
#12
Guinevere S M Kammeijer, Bas C Jansen, Isabelle Kohler, Anthonius A M Heemskerk, Oleg A Mayboroda, Paul J Hensbergen, Julie Schappler, Manfred Wuhrer
Sialylation is a glycosylation feature that occurs in different linkages at the non-reducing end of a glycan moiety, the linkage isomers are often differentially associated with various biological processes. Due to very similar physico-chemical properties, the separation of isomeric sialylated glycopeptides remains challenging but of utmost importance in the biomedicine and biotechnology, including biomarker discovery, glyco-engineering and biopharmaceutical characterization. This study presents the implementation of a high-resolution separation platform based on capillary electrophoresis - mass spectrometry (CE-MS) allowing for the selective analysis of α2,3- and α2,6-sialylated glycopeptides...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28620050/engineering-the-fragment-crystallizable-fc-region-of-human-igg1-multimers-and-monomers-to-fine-tune-interactions-with-sialic-acid-dependent-receptors
#13
Patricia A Blundell, Ngoc Phuong Lan Le, Joel Allen, Yasunori Watanabe, Richard J Pleass
Multimeric fragment crystallizable (Fc) regions and Fc-fusion proteins are actively being explored as biomimetic replacements for intravenous immunoglobulin (IVIG) therapy, which is deployed to manage many diseases and conditions but is expensive and not always efficient. The Fc region of human IgG1 (IgG1-Fc) can be engineered into multimeric structures (hexa-Fcs) that bind their cognate receptors with high avidity. The critical influence of the unique N-linked glycan attached at Asn-297 on the structure and function of IgG1-Fc is well documented; however, whether the N-linked glycan has a similarly critical role in multimeric, avidly binding Fcs is unknown...
June 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28619634/enhancement-of-siglec-8-expression-predicts-adverse-prognosis-in-patients-with-clear-cell-renal-cell-carcinoma
#14
Chenzhang Ou, Li Liu, Jiajun Wang, Siyuan Dai, Yang Qu, Ying Xiong, Wei Xi, Jiejie Xu, Jianming Guo
PURPOSE: Sialic acid-binding immunoglobulin-like lectins (siglecs) family has important functions in tumor progression. The purpose of our study is to figure out the correlation between the expression level of Siglec-8 and prognosis of patients with clear cell renal cell carcinoma (ccRCC), and then to predict the overall survival (OS) via a novel nomogram. MATERIALS AND METHODS: A group of patients (n = 267) histologically diagnosed with ccRCC from Zhongshan Hospital were included into our study...
June 12, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/28619036/monocyte-differentiation-and-macrophage-priming-are-regulated-differentially-by-pentraxins-and-their-ligands
#15
Darrell Pilling, Elkin Galvis-Carvajal, Tejas R Karhadkar, Nehemiah Cox, Richard H Gomer
BACKGROUND: Circulating bone marrow-derived monocytes can leave the blood, enter a tissue, and differentiate into M1 inflammatory, M2a remodeling/fibrotic, or M2c/Mreg resolving/immune-regulatory macrophages. Macrophages can also convert from one of the above types to another. Pentraxins are secreted proteins that bind to, and promote efficient clearance of, microbial pathogens and cellular debris during infection, inflammation, and tissue damage. The pentraxins C-reactive protein (CRP), serum amyloid P (SAP), and pentraxin-3 (PTX3) can also bind a variety of endogenous ligands...
June 15, 2017: BMC Immunology
https://www.readbyqxmd.com/read/28615470/identification-of-the-group-b-streptococcus-capsule-type-using-a-dual-phenotypic-genotypic-assay
#16
Areej Alhhazmi, Armaan Pandey, Gregory J Tyrrell
The Group B streptococcus (GBS) capsular polysaccharide (CPS) is an important virulence factor which is also used for GBS typing. There are 10 CPS types (Ia, Ib and II-IX). GBS that do not phenotypically type are considered non-typable. All genes required for CPS synthesis are found on the GBS cps operon, which contains a highly variable CPS-determining region (cpsG-cpsK).The objective of this study was development of an assay to detect sialic acid on the GBS cell surface followed by a genotypic PCR CPS typing assay...
June 14, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28614672/composition-and-variation-of-macronutrients-immune-proteins-and-human-milk-oligosaccharides-in-human-milk-from-nonprofit-and-commercial-milk-banks
#17
Laura Meredith-Dennis, Gege Xu, Elisha Goonatilleke, Carlito B Lebrilla, Mark A Underwood, Jennifer T Smilowitz
BACKGROUND: When human milk is unavailable, banked milk is recommended for feeding premature infants. Milk banks use processes to eliminate pathogens; however, variability among methods exists. Research aim: The aim of this study was to compare the macronutrient (protein, carbohydrate, fat, energy), immune-protective protein, and human milk oligosaccharide (HMO) content of human milk from three independent milk banks that use pasteurization (Holder vs. vat techniques) or retort sterilization...
June 1, 2017: Journal of Human Lactation: Official Journal of International Lactation Consultant Association
https://www.readbyqxmd.com/read/28614533/effect-of-fluoride-on-salivary-immunoglobulins-and-sialic-acid
#18
Kadriye Görkem Ulu Güzel, Zuhal Kirzioğlu, Ali Kudret Adiloğlu, Münciye Semra Özay Ertürk
Objective: The aim of our study was to evaluate the effect of fluoride on salivary immunoglobulin and sialic acid levels in children with dental fluorosis and healthy teeth who live in places with high fluoride concentration in drinking water. Method: Fifty-one (51) healthy children between 6 and 12 years old with no caries were randomly selected from primary schools enrolled in the dental-care program operated by the Department of Pediatric Dentistry. The children were divided into two groups: group I comprised 26 children with dental fluorosis [Thylstrup-Fejerskov Dental Fluorosis Index (TFI) = 4] who lived in Isparta (2...
April 2017: Revista da Associação Médica Brasileira
https://www.readbyqxmd.com/read/28608355/-neuronal-glycolipids-regulate-glial-cell-division-negatively-during-development-and-following-a-lesion
#19
REVIEW
M Nieto-Sampedro, V C Muneton-Gomez
Glial cells in the central nervous system of adult mammals outnumber neurons 10-fold. Their number remains stationary throughout adulthood, controlled by the concomitant presence of mitogens and mitogen inhibitors. The most abundant inhibitor, neurostatin, is ganglioside GD1b O-acetylated on hydroxyl 9 of its outermost sialic acid. Neurostatin inhibited the proliferation of primary microglia and astroblasts in culture (cytostatic) as well as both rodent and human glioma cells (cytotoxic) at nanomolar concentrations...
June 16, 2017: Revista de Neurologia
https://www.readbyqxmd.com/read/28606317/molecular-occurrence-of-trypanosomes-erythrocyte-and-serum-sialic-acid-concentrations-of-muturu-and-bunaji-cattle-in-benue-state-nigeria
#20
Samuel Ode, Mathew Adamu, Moeti Taioe, Oriel Thekisoe, Sani Adamu, Daniel I Saror
One hundred each, of Muturu and Bunaji cattle were screened, using polymerase chain reaction (PCR), for trypanosomes in Makurdi and Gboko Local Government Areas of Benue State, Nigeria. Erythrocyte surface sialic acid (ESSA) and free serum sialic acid (FSSA) concentrations were determined and compared in both breeds with the aim of providing baseline data for research and diagnostic purposes. Five per cent (5%) and 23% of the Muturu and Bunaji cattle, respectively, were positive for trypanosomes. The result at p=0...
August 15, 2017: Veterinary Parasitology
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