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https://www.readbyqxmd.com/read/29679653/effects-of-selective-serotonin-reuptake-inhibitors-ssris-on-human-villous-trophoblasts-syncytialization
#1
Hélène Clabault, Denis Flipo, Jean Guibourdenche, Thierry Fournier, Thomas Sanderson, Cathy Vaillancourt
Selective serotonin-reuptake inhibitors (SSRIs) are the most commonly prescribed antidepressants during pregnancy. The human placenta is a highly specialized organ supporting normal growth and development of the fetus. Therefore, this study aims to analyze the effects of SSRIs on villous cytotrophoblasts cells, using BeWo cells and human placental trophoblast cells in primary culture. The SSRIs fluoxetine and its metabolite norfluoxetine, sertraline and venlafaxine did not affect BeWo cell proliferation and viability, nor the percentage of M30-positive (apoptotic) primary trophoblast cells...
April 18, 2018: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/29679053/pregnancy-associated-diamine-oxidase-originates-from-extravillous-trophoblasts-and-is-decreased-in-early-onset-preeclampsia
#2
Philipp Velicky, Karin Windsperger, Karin Petroczi, Sophie Pils, Birgit Reiter, Tamara Weiss, Sigrid Vondra, Robin Ristl, Sabine Dekan, Christian Fiala, David E Cantonwine, Thomas F McElrath, Bernd Jilma, Martin Knöfler, Thomas Boehm, Jürgen Pollheimer
Human extravillous trophoblast (EVT) invasion of the pregnant uterus constitutes a pivotal event for the establishment of the maternal-fetal interface. Compromised EVT function manifesting in inadequate arterial remodeling is associated with the severe pregnancy disorder early-onset preeclampsia (eoPE). Recent studies suggest that EVTs invade the entire uterine vasculature including arteries, veins and lymphatics in the first trimester of pregnancy. We therefore hypothesized that EVT-derived factors accumulate in the circulation of pregnant women early in gestation and may serve to predict eoPE...
April 20, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29673971/changes-in-trophoblasts-gene-expression-in-response-to-perchlorate-exposition
#3
De la Peña Sol, Santiago-Roque Isela, Olivo-Vidal Zendy, Navarro-Meza Mónica, Xochihua-Rosas Irene, Arroyo-Helguera Omar
Contaminated water with chlorates is a public health problem associated with iodine deficiency. Epidemiological evidence shows that iodine deficiency is a risk factor for preeclampsia (PE). In this study we use human BeWo trophoblast cells exposed to perchlorate (KClO4 ) and changes in gene expression were analyzed by microarrays, quantitative RT-PCR (qRT-PCR) and immunoblot. The microarray analysis identified 48 transcripts up-regulated and 112 down-regulated in comparison with non-exposed trophoblast. The qRT-PCR analysis confirmed changes in GAS7, PKP2, Emilin, Dynatic 3, protocadherins 11, 15, gamma A12, EGFR, SAFB1, ACE2, ANXA2, Apoliprotein E, SREBF1, and C/EBP-β...
April 16, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/29673680/two-cesarean-deliveries-after-hemi-hysterectomy-due-to-gestational-trophoblastic-neoplasia
#4
Kentaro Kai, Yasushi Kawano, Mitsutake Yano, Mamiko Okamoto, Eiichi Hori, Kaei Nasu, Hisashi Narahara
OBJECTIVE: Although uterine didelphys per se is not associated with an impaired ability to conceive, the association between uterine anomalies and gestational trophoblastic neoplasia (GTN) remains unclear. The management of chemotherapy-resistant GTN in women with uterine didelphys raises a new issue regarding whether to perform a hemi-hysterectomy. CASE REPORT: A 23-year-old, gravida 1, para 0 Japanese woman was referred with a failed intermittent cervical dilatation for hematometra...
April 2018: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29673663/hmgb1-rage-signaling-pathway-in-pprom
#5
Huan Yan, Linlin Zhu, Zhan Zhang, Hong Li, Pengyun Li, Yan Wang, Maodong Leng
OBJECTIVE: Increased inflammation of the placenta is considered as a risk factor and a promoter of preterm premature rupture of the membranes (pPROM). High-mobility group box 1 (HMGB1) is a recently identified inflammatory cytokine, and HMGB1-RAGE signaling pathway has been associated with many pathophysiological processes. This study aims to reveal the mechanisms of HMGB1-RAGE signaling pathway in pPROM. MATERIALS AND METHODS: The mRNA levels of relative gene of HMGB1 pathway, HMGB1, RAGE, NF-κBp65, MMP-9 and MMP-2, were analyzed by real-time PCR in placentas collected from 60 normal term women, 60 women with PROM and 60 women with pPROM...
April 2018: Taiwanese Journal of Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29673026/in-vitro-effects-of-seng-and-tgf-%C3%AE-on-human-umbilical-vein-endothelial-cells-and-trophoblasts
#6
Ketao Wang, Caihua Lu, Yanpo Liu, Ye Tao
AIM: The aim of this study was to evaluate the in vitro effects of sEng and TGF-β on human umbilical vein endothelial cells (HUVECs) and trophoblasts. METHODS: HUVECs and trophoblasts were treated with 1, 5, 10, 15, 30, 50, 80 and 100 μg/L sEng, TGF-β or (sEng + TGF-β) for 6, 12, 24, 36, 48 and 72 h, respectively. sEng, TGF-β and NO levels in culture media of HUVECs were measured by ELISA. Survival rates of HUVECs and trophoblasts were detected by 3-[4, 5-dimethylthiazol-2-yl]-2, 5 diphenyltetrazolium bromide (MTT) assay...
April 19, 2018: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/29670668/association-of-maternal-and-fetal-single-nucleotide-polymorphisms-in-metalloproteinase-mmp1-mmp2-mmp3-and-mmp9-genes-with-preeclampsia
#7
Agata Sakowicz, Michalina Lisowska, Lidia Biesiada, Magda Rybak-Krzyszkowska, Agnieszka Gach, Bartosz Sakowicz, Mariusz Grzesiak, Hubert Huras, Tadeusz Pietrucha
Background: Metalloproteinases (MMPs) play a pivotal role during the process of trophoblast invasion and placentation. The appearance of five functional single-nucleotide polymorphisms (SNP) in the genes of the metalloproteinases most commonly implicated in the implantation process may influence the development of preeclampsia. Methods: Blood samples were collected from 86 mothers and 86 children after preeclampsia and 85 mothers and 85 children with uncomplicated pregnancies...
2018: Disease Markers
https://www.readbyqxmd.com/read/29668580/metastatic-epithelioid-trophoblastic-tumor-of-the-lung-a-case-report
#8
Wangjun Lei, Fangbiao Zhang, Chunhui Zheng, Chun Zhao, Shaosong Tu, Yingwei Bao
RATIONALE: Epithelioid trophoblastic tumor (ETT) is a very rare form of gestational trophoblastic disease (GTD) which arises from neoplastic proliferation of intermediate trophoblasts. Metastatic ETT of the lung is extremely rare in postmenopausal women. PATIENT CONCERNS: Here we describe a 50-year-old woman with a metastatic ETT of the lung showing increasing tracer uptake at PET/CT. DIAGNOSIS: Hematoxylin-eosin staining showed a tumor composed of nests of epithelioid cells with necrotic debris and peritumoral hyaline-like material...
April 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29665822/toxoplasma-gondii-dense-granule-protein-15-induces-apoptosis-in-choriocarcinoma-jeg-3-cells-through-endoplasmic-reticulum-stress
#9
Wei Wei, Fangfang Zhang, He Chen, Yuanyuan Tang, Tian Xing, Qingli Luo, Li Yu, Jian Du, Jilong Shen, Linjie Zhang
BACKGROUND: Toxoplasma gondii, a single-celled parasite commonly found in mammals, has been shown to induce trophoblast cell apoptosis and subsequently cause fetal damage and abortion. Although dense granule protein 15 (GRA15) has been identified as a key component in innate immunity to T. gondii infection and its pathogenesis, its role in host cell apoptosis remains unclarified. METHODS: Type II GRA15 (GRA15II ) cDNA was inserted into a plasmid encoding enhanced green fluorescent protein (pEGFP)...
April 17, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29665647/mir-362-3p-regulates-cell-proliferation-migration-and-invasion-of-trophoblastic-cells-under-hypoxia-through-targeting-pax3
#10
Nan Wang, Yaling Feng, Jianjuan Xu, Jinfang Zou, Minghua Chen, Yue He, Huan Liu, Min Xue, Yanfang Gu
Preeclampsia (PE), a common obstetrical disorder, is one of the leading causes of pregnancy associated death. PE is closely linked with impaired migration and invasion ability of trophoblastic cells. miR-362-3p recently received our particular attention due not only to its aberrant expression in the placentas of patients with PE, but also to its important roles in regulating migration and invasion of various cells. This study was thus conducted to investigate the roles of miR-362-3p in PE and the related mechanism...
March 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29665170/breast-carcinoma-in-pregnancy-with-spheroid-like-placental-metastases-a-case-report
#11
Karolin Froehlich, Hanne Stensheim, Udo R Markert, Gitta Turowski
Breast cancer is one of the most common malignancies diagnosed in pregnancy. Although the tumor is often detected at an advanced stage, placental metastases are rare. Here, we describe the case of a woman with breast cancer recurrence during pregnancy and subsequent metastases. The focus of this study is the large amount of placenta metastases, which have been analyzed immunohistochemically. Staining with trophoblast markers (placenta alkaline phosphatase, beta human chorionic gonadotropin and human placental lactogen) showed the strict localization of metastases in the intervillous space without invasion into fetal tissue...
April 17, 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/29663570/lefty-promotes-the-proliferation-and-invasion-of-trophoblast-cells-by-inhibiting-nodal-expression
#12
Hong Li, Rui Cao, Liang Bai, Xin-Min Qiao, Yong-Qin Zhao
Trophoblast cells play a vital role in embryo implantation. Exploring cytokines secreted by trophoblast cells will be significant in revealing the molecular mechanism of embryo implantation. In vitro-cultured trophoblast cells treated with Lefty revealed that Lefty could downregulate the expression of Nodal while promoting the expression of MMP-2 and MMP-9. Thus, Lefty may promote the proliferation and invasion of trophoblast cells, thereby reducing the amount of apoptosis of these cells. Lefty siRNA knockdown or overexpression of Nodal showed opposite effects to treatment with Lefty...
April 16, 2018: Cell Biology International
https://www.readbyqxmd.com/read/29662820/effects-of-selenium-on-the-survival-and-invasion-of-trophoblasts
#13
Jee Yoon Na, Jin Seok, Sohae Park, Jung Seok Kim, Gi Jin Kim
Objective: Placental oxidative stress is known to be a factor that contributes to pregnancy failure. The aim of this study was to determine whether selenium could induce antioxidant gene expression and regulate invasive activity and mitochondrial activity in trophoblasts, which are a major cell type of the placenta. Methods: To understand the effects of selenium on trophoblast cells exposed to hypoxia, the viability and invasive activity of trophoblasts were analyzed...
March 2018: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29662139/decreased-expression-of-jhdmid-in-placenta-is-associated-with-preeclampsia-through-hla-g
#14
Shouling Luo, Jiangnan Pei, Xiaotian Li, Weirong Gu
The exact mechanism of preeclampsia (PE) remains unclear, accumulating researches have indicated multiple epigenetic factors relate to PE and histone methylation plays a crucial role in modifying the gene expression. So we aimed to confirm that abnormal expression of histone demethylase JHDM1D contributes to PE and lower expression of HLA-G in PE. We tested the expression of JHDM1D, H3K9me2, and H3K27me2 in the placentas of PE and normal control (NC)women who had a healthy pregnancy with Immunohistochemistry and we found that JHDM1D, H3K9me2, and H3K27me2 were all mainly expressed in the nuclei of the extra-villous trophoblasts (EVTs)...
April 6, 2018: Journal of Human Hypertension
https://www.readbyqxmd.com/read/29660299/infection-induced-thrombin-production-a-potential-novel-mechanism-for-preterm-premature-rupture-of-membranes-pprom
#15
Liping Feng, Terrence K Allen, William P Marinello, Amy P Murtha
BACKGROUND: Preterm premature rupture of membranes (PPROM) is a leading contributor to maternal and neonatal morbidity and mortality. Epidemiologic and experimental studies have demonstrated that thrombin causes fetal membrane weakening and subsequently PPROM. Although blood is suspected as the likely source of thrombin in fetal membranes and amniotic fluid of patients with PPROM, this has not been proven. Ureaplasma Parvum (U. parvum) is emerging as a pathogen involved in prematurity, including PPROM, but until now, prothrombin production directly induced by bacteria in fetal membranes has not been described...
April 13, 2018: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29659791/human-placental-growth-hormone-variant-in-pathological-pregnancies
#16
Shutan Liao, Mark H Vickers, Joanna L Stanley, Philip N Baker, Jo K Perry
Growth hormone (GH), an endocrine hormone primarily secreted from the anterior pituitary, stimulates growth, cell reproduction and regeneration and is a major regulator of postnatal growth. Humans have two GH genes which encode two versions of GH proteins: a pituitary version (GH-N/GH1), and a placental GH variant (GH-V/GH2) which is expressed in the syncytiotrophoblast and extravillous trophoblast cells of the placenta. During pregnancy, placental GH replaces pituitary GH in the maternal circulation at mid-late gestation as the major circulating form of GH...
April 6, 2018: Endocrinology
https://www.readbyqxmd.com/read/29659388/imaging-of-metastatic-epithelioid-trophoblastic-tumor-with-18f-fdg-pet-ct
#17
Tirth Patel, Jorge Oldan
Epithelioid trophoblastic tumors are exceptionally rare malignant forms of gestational trophoblastic neoplasia arising from the placenta. We present here a case of a 33-year-old woman with an epithelioid trophoblastic tumor with metastatic disease to the lungs that was initially thought to represent cervical carcinoma. After appropriate diagnosis and treatment, the tumor responded to therapy and was in remission. The cancer recurred 5 years later with new lung metastases. F-FDG PET/CT was used for initial tumor staging, to evaluate treatment response, and to help detect the recurrence...
April 13, 2018: Clinical Nuclear Medicine
https://www.readbyqxmd.com/read/29658922/three-dimensional-rendering-and-analysis-of-immunolabeled-clarified-human-placental-villous-vascular-networks
#18
George Merz, Valerie Schwenk, Ruchit Shah, Carolyn Salafia, Phillip Necaise, Michael Joyce, Tom Villani, Michael Johnson, Nick Crider
Nutrient and gas exchange between mother and fetus occurs at the interface of the maternal intervillous blood and the vast villous capillary network that makes up much of the parenchyma of the human placenta. The distal villous capillary network is the terminus of the fetal blood supply after several generations of branching of vessels extending out from the umbilical cord. This network has a contiguous cellular sheath, the syncytial trophoblast barrier layer, which prevents mixing of fetal blood and the maternal blood in which it is continuously bathed...
March 29, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29658087/complex-analysis-of-total-and-fetal-dna-and-cytokines-in-blood-plasma-of-pregnant-women-with-preeclampsia
#19
A M Krasnyi, M I Gracheva, A A Sadekova, V V Vtorushina, I S Balashov, N E Kan, P I Borovikov, L V Krechetova, V L Tyutyunnik
We performed a complex analysis of total and fetal extracellular DNA, 8 cytokines (IL-2, IL-4, IL-6, IL-8, IL-10, granulocyte-macrophage CSF, IFNγ, and TNFα) in blood plasma obtained from women with preeclampsia prior to labor onset. Total (sensitivity 89.47%, specificity 93.75%) and fetal extracellular DNA (sensitivity 73.68%, specificity 87.5%) were the most accurate parameters determining preeclampsia. We revealed a high correlation (p=3×10-6 ) between total and fetal extracellular DNA levels in the group of preeclampsia...
April 16, 2018: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/29651447/impaired-gal-9-dysregulates-the-pbmc-induced-th1-th2-imbalance-in-abortion-prone-matings
#20
Mengzhou He, Ming Jiang, Yuan Zhou, Fanfan Li, Meitao Yang, Yao Fan, Yin Xie, Rajluxmee Beejadhursing, Ling Feng, Dongrui Deng
Recurrent miscarriage is defined as the loss of 3 or more consecutive pregnancies; however, the underlying immunologic mechanisms that trigger pregnancy loss remain largely unelucidated. Galectin-9 (Gal-9) may modulate a variety of biologic functions and play an important role in Th1/Th2 immune deviation. To analyze the mechanism of Gal-9 in abortion, we used the classical abortion-prone mouse model (DBA/2-mated CBA/J mice) to detect the expression of Gal-9 at the maternal-fetal interface. We also mimicked the immune environment of pregnancy by culturing trophoblast cells with peripheral blood mononuclear cells (PBMCs) to explore how Gal-9 might be involved in the pathogenesis of abortion...
2018: Journal of Immunology Research
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