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https://www.readbyqxmd.com/read/29911998/hif2%C3%AE-in-the-uterine-stroma-permits-embryo-invasion-and-luminal-epithelium-detachment
#1
Leona Matsumoto, Yasushi Hirota, Tomoko Saito-Fujita, Norihiko Takeda, Tomoki Tanaka, Takehiro Hiraoka, Shun Akaeda, Hidetoshi Fujita, Ryoko Shimizu-Hirota, Shota Igaue, Mitsunori Matsuo, Hirofumi Haraguchi, Mayuko Saito-Kanatani, Tomoyuki Fujii, Yutaka Osuga
Although it has been reported that hypoxia inducible factor 2 α (Hif2a), a major transcriptional factor inducible by low oxygen tension, is expressed in the mouse uterus during embryo implantation, its role in pregnancy outcomes remains unclear. This study aimed to clarify functions of uterine HIF using transgenic mouse models. Mice with deletion of Hif2a in the whole uterus (Hif2a-uKO mice) showed infertility due to implantation failure. Supplementation with progesterone (P4) and leukemia inhibitory factor (LIF) restored decidual growth arrest and aberrant position of implantation sites in Hif2a-uKO mice, respectively, but did not rescue pregnancy failure...
June 18, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29910815/-toxoplasma-chinese-1-strain-of-wh3%C3%AE-rop16-i-iii-gra15-ii-genetic-background-contributes-to-abnormal-pregnant-outcomes-in-murine-model
#2
Cong Wang, Weisheng Cheng, Qian Yu, Tian Xing, Shoubin Chen, Lei Liu, Li Yu, Jian Du, Qingli Luo, Jilong Shen, Yuanhong Xu
Toxoplasma gondii infection evokes a strong Th1-type response with interleukin (IL)-12 and interferon (IFN)-γ secretion. Recent studies suggest that the infection of pregnant mice with T. gondii may lead to adverse pregnancy results caused by subversion of physiological immune tolerance at maternofetal interface rather than direct invasion of the parasite. Genotype-associated dense granule protein GRA15II tends to induce classically activated macrophage (M1) differentiation and subsequently activating NK, Th1, and Th17 cells whereas rhoptry protein ROP16I/III drives macrophages to alternatively activated macrophage (M2) polarization and elicits Th2 immune response...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29909746/trophoblast-derived-cxcl16-decreased-granzyme-b-production-of-decidual-%C3%AE-%C3%AE-t-cells-and-promoted-bcl-xl-expression-of-trophoblasts
#3
Deng-Xuan Fan, Wen-Jie Zhou, Li-Ping Jin, Ming-Qing Li, Xiang-Hong Xu, Cong-Jian Xu
BACKGROUND: Decidual γδ T cells are known to regulate the function of trophoblasts at the maternal-fetal interface; however, little is known about the molecular mechanisms of cross talk between trophoblast cells and decidual γδ T cells. METHODS: Expression of chemokine C-X-C motif ligand 6 (CXCL16) and its receptor CXCR6 was evaluated in first-trimester human villus and decidual tissues by immunohistochemistry. γδ T cells were isolated from first-trimester human deciduae and cocultured with JEG3 trophoblast cells...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29908721/time-dependent-expression-pattern-of-cytochrome-p450-epoxygenases-and-soluble-epoxide-hydrolase-in-normal-human-placenta
#4
K Cizkova, Z Tauber
CYP2C and CYP2 J enzymes, commonly named as cytochrome P450 (CYP) epoxygenases, convert arachidonic acid to four regioisomeric epoxyeicosatrienoic acids (EETs), biologically active eicosanoids with many functions in organism. EETs are rapidly hydrolysed to less active dihydroxyeicosatrienoic acids (DHETs) by soluble epoxide hydrolase (sEH). We investigated spatio-temporal expression pattern of CYP2C8, CYP2C9, CYP2 J2 and sEH in normal human placenta by immunohistochemical method. In the villous trophoblast, CYP2C8 was the most abundant protein...
June 13, 2018: Acta Histochemica
https://www.readbyqxmd.com/read/29908644/cadmium-induces-inflammatory-cytokines-through-activating-akt-signaling-in-mouse-placenta-and-human-trophoblast-cells
#5
Jun Hu, Hua Wang, Yong-Fang Hu, Xiao-Feng Xu, Yuan-Hua Chen, Mi-Zhen Xia, Cheng Zhang, De-Xiang Xu
INTRODUCTION: Several reports demonstrated that cadmium (Cd) had proinflammatory activities. The present study aimed to investigate whether Cd induces inflammatory cytokines in mouse placenta and human trophoblast cells. METHODS: Human JEG-3 cells were treated with different concentration of CdCl2 (0-50 μM) or CdCl2 (25 μM) for different times. The pregnant mice were administered with CdCl2 (3.0 mg/kg, i.p.) on GD15. RESULTS: TNF-α, IL-8 and IL-6 mRNAs were elevated in CdCl2 -treated JEG-3 cells...
May 2018: Placenta
https://www.readbyqxmd.com/read/29908641/gestational-changes-in-prmt1-expression-of-murine-placentas
#6
Anna Sato, Jun-Dal Kim, Hayase Mizukami, Misaki Nakashima, Koichiro Kako, Junji Ishida, Atsuo Itakura, Satoru Takeda, Akiyoshi Fukamizu
INTRODUCTION: In mammals, the placenta is an organ that is required to maintain the development of fetus during pregnancy. Although the proper formation of placenta is in part regulated by the post-translational modifications of proteins, little is known regarding protein arginine methylation during placental development. Here, we characterized developmental expression of protein arginine methyltransferase 1 (PRMT1) in mouse placentas. METHODS: Expression levels of PRMT1 mRNA and protein in placentas were investigated using the real-time quantitative PCR and Western blot, respectively...
May 2018: Placenta
https://www.readbyqxmd.com/read/29908639/a-bioinformatics-transcriptome-meta-analysis-highlights-the-importance-of-trophoblast-differentiation-in-the-pathology-of-hydatidiform-moles
#7
Christophe Desterke, Rima Slim, Jean-Jacques Candelier
INTRODUCTION: Hydatidiform mole (HM) is an aberrant human pregnancy with abnormal trophoblastic development, migration/invasion of the extravillous trophoblast in the decidua. These abnormalities are established in a hypoxic environment during the first trimester of gestation. METHODS: Using text mining, we identified 72 unique genes that are linked to HM (HM-linked genes) that we studied by bioinformatic analysis in publicly available transcriptomes of primary chorionic villous cells (cytotrophoblast, syncytiotrophoblast, extravillous trophoblast, and arterial and venous endothelial) isolated from normal placentas or established trophoblastic cell lines cultured under different oxygen concentrations...
May 2018: Placenta
https://www.readbyqxmd.com/read/29908376/the-increased-level-of-tspan5-in-villi-suggests-more-proliferation-and-invasiveness-of-trophoblasts-in-tubal-pregnancy
#8
Tianyang Gao, Yingqiu Liang, Haiyu Tang, Li Quan
OBJECTIVE: This study was to determinate the expression of Tspan5 in tubal ectopic implantation sites and to explore the correlation of the expressive level of Tspan5 at maternal-fetal interface and the occurrence of tubal ectopic pregnancy. STUDY DESIGN: This is a retrospective study. Trophoblastic and endometrial tissues were collected from tubal ectopic pregnancy(Total of 40), and intrauterine pregnancy(Total of 41), who had voluntary abortion, non-pregnancy women(Total of 12), who recieved an diagnostic uterine curettage before IVF-ET for male infertility...
June 5, 2018: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://www.readbyqxmd.com/read/29907882/relevance-of-placental-type-i-interferon-beta-regulation-for-pregnancy-success
#9
Ja-Young Kwon, Paulomi Aldo, Yuan You, Jiahui Ding, Karen Racicot, Xiaoyan Dong, John Murphy, Guy Glukshtad, Michelle Silasi, Jian Peng, Li Wen, Vikki M Abrahams, Roberto Romero, Gil Mor
Pregnancy is a unique immunologic and microbial condition that requires an adequate level of awareness to provide a fast and protective response against pathogens as well as to maintain a state of tolerance to paternal antigens. Dysregulation of inflammatory pathways in the placenta triggered by pathogens is one of the main factors responsible for pregnancy complications. Type I IFNs are key molecules modulating immune responses at the level of the placenta and are crucial for protection of the pregnancy via their antiviral and immune modulatory properties...
June 15, 2018: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/29905829/two-hormones-for-one-receptor-evolution-biochemistry-actions-and-pathophysiology-of-lh-and-hcg
#10
Livio Casarini, Daniele Santi, Giulia Brigante, Manuela Simoni
Luteinizing hormone (LH) and chorionic gonadotropin (CG) are glycoproteins fundamental for sexual development and reproduction. Since they act on the same receptor (LHCGR), there is a general consensus that LH and hCG are equivalent. However, separate evolution of LHβ and hCGβ subunits occurred in primates, resulting in two molecules sharing ∼85% identity and regulating different physiological events. Pituitary, pulsatile LH production results in a ∼90 min half-life molecule targeting the gonads, to regulate gametogenesis and androgen synthesis...
June 13, 2018: Endocrine Reviews
https://www.readbyqxmd.com/read/29904999/p57-kip-2-immunostaining-for-diagnosis-of-hydatidiform-mole
#11
B Kaur, N J Sebire
Accurate diagnosis and sub-classification of hydatidiform moles (HM) and distinction from non-molar (NM) gestations is important, since risk of persistent gestational trophoblastic disease (pGTD) and therefore clinical management differ. P57KIP 2 immunostaining has been widely described as an ancillary test to distinguish complete (CHM) and partial (PHM) hydatidiform moles, but cannot discern PHM from non-molar gestations (NM) or identify androgenetic vs biparental CHM, which may be associated with increased recurrence risk...
June 15, 2018: BJOG: An International Journal of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/29901071/curcumin-inhibits-cell-proliferation-and-motility-via-suppression-of-trop2-in-bladder-cancer-cells
#12
Lianhua Zhang, Guoliang Yang, Ruiyun Zhang, Liang Dong, Haige Chen, Juanjie Bo, Wei Xue, Yiran Huang
Bladder cancer (BC) has become a serious health prob-lem and represents the second most commonly diagnosed urological tumor. Curcumin is a principal active natural component of turmeric and has long been used in Asia as a traditional herbal medicine. Curcumin suppresses cell growth in various types of cancer, including BC, by regulating numerous molecular signaling pathways. The human trophoblast cell surface antigen 2 (Trop2) belongs to the tumor-associated calcium signal transducer gene family. Trop2 has been described as a cancer driver and is deregulated in various types of cancer...
May 30, 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29898663/cell-type-specific-analysis-of-transcriptome-changes-in-the-porcine-endometrium-on-day-12-of-pregnancy
#13
Shuqin Zeng, Jochen Bick, Susanne E Ulbrich, Stefan Bauersachs
BACKGROUND: Along with trophoblast elongation (Days 10 to 12), estradiol is secreted in increasing amounts for recognition of pregnancy. Endometrial secretions driven by ovarian progesterone and conceptus signals are essential for conceptus growth and development. Results of transcriptome analyses of whole endometrial tissue samples in the pig indicated the need for cell type-specific endometrial gene expression analysis for a better understanding of transcriptome changes associated with establishment of pregnancy...
June 14, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29897391/a-case-series-of-five-patients-with-pure-or-mixed-gestational-epithelioid-trophoblastic-tumors-and-a-literature-review-on-mixed-tumors
#14
Ka Yu Tse, Keith Wan Hang Chiu, Karen Kar Loen Chan, Mandy Man Yee Chu, Siew Fei Ngu, Annie Nga Yin Cheung, Hextan Yuen Sheung Ngan, Philip Pun Ching Ip
Objectives: To review the clinicopathologic features of five patients with epithelioid trophoblastic tumor (ETT). Methods: Characteristics of patients diagnosed with ETT in 2000 to 2012 were reviewed. Results: Among 190 patients with gestational trophoblastic neoplasia (GTN), two had pure ETT and three had mixed ETT and choriocarcinoma. The median age was 32.5 years. All the patients had localized disease in the uterus. One patient with pure ETT had a recurrence in the ureter 6 years after the initial treatment...
June 11, 2018: American Journal of Clinical Pathology
https://www.readbyqxmd.com/read/29896727/-pre-implantation-genetic-screening-of-discarded-embryos-through-whole-genome-amplification-and-next-generation-sequencing
#15
Yurong Zhu, Chunling Liu, Sheng Yang, Peng Liu, Guosong Shen
OBJECTIVE: To assess the value of whole-genome amplification (WGA) and next generation sequencing (NGS) for the pre-implantation screening of discarded embryos. METHODS: In total 476 discarded embryos were collected. After continued culture, 23 high-quality blastocysts were obtained. Blastocysts graded as 4BC or above based on Gardner classification were subjected for blastula biopsy. Five to ten nourish ectoderm cells were hatched with a biopsy needle. Following WGA and NGS, deletion and/or duplication of chromosomal fragments and numerical chromosomal aberrations were analyzed...
June 10, 2018: Zhonghua Yi Xue Yi Chuan Xue za Zhi, Zhonghua Yixue Yichuanxue Zazhi, Chinese Journal of Medical Genetics
https://www.readbyqxmd.com/read/29896240/effects-of-forkhead-box-protein-m1-on-trophoblast-invasion-and-its-role-in-preeclampsia-development
#16
Xuena Cui, Jin'e Xu, Yuzhi Ji, Xiuhong Song, Junhuan Wang, Lijuan Zhang, Shengmei Yang, Yuanhua Ye
The present study aimed to investigate the expression of the forkhead box protein M1 (FOXM1) in the placenta of patients with preeclampsia, and its effect on trophoblasts. A total of 28 patients with preeclampsia and 30 patients without preeclampsia (controls) who underwent cesarean section and were admitted to the Affiliated Hospital of Qingdao University between June 2013 and September 2016 were enrolled in the present study. The expression of FOXM1 in placental tissues was examined by reverse transcription-quantitative polymerase chain reaction, western blotting and immunohistochemistry...
July 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29895936/int6-eif3e-silencing-promotes-placenta-angiogenesis-in-a-rat-model-of-pre-eclampsia
#17
Qin Li, Baolin Yao, Alexander Endler, Li Chen, Futoshi Shibasaki, Haidong Cheng
We investigated whether stable eukaryotic translation initiation factor 3e/inter 6 (eIF-3e/Int6) RNA-silencing (siRNA-Int6) can ameliorate pre-eclampsia (PE) by promoting angiogenesis in an N-nitro-L-arginine methyl ester (L-NAME)-induced rat pre-eclampsia (PE) model. Twenty-four pregnant female Sprague-Dawley rats were allocated into 4 groups, including controls (Con) without any treatment, and 18 from gestational day (GD) 7 to GD17 L-NAME-treated rats, which were divided into stable siRNA-Int6 transfected (siRNA-Int6), negative vector control siRNA (NC-siRNA) and PE control (PE-Con) groups...
June 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29895842/human-trophoblast-stem-cell-self-renewal-and-differentiation-role-of-decorin
#18
Pinki Nandi, Hyobin Lim, Eloy Jose Torres-Garcia, Peeyush K Lala
The origin and regulation of stem cells sustaining trophoblast renewal in the human placenta remain unclear. Decorin, a leucine-rich proteoglycan restrains trophoblast proliferation, migration/invasiveness and endovascular differentiation, and local decorin overproduction is associated with preeclampsia (PE). Here, we tested the role of decorin in human trophoblast stem cell self-renewal and differentiation, using two models: an immortalized first trimester trophoblast cell line HTR-8/SVneo (HTR) and freshly isolated primary trophoblast (p-trophoblast) from early first trimester (6-9 weeks) placentas...
June 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29891454/-role-of-endoplasmic-reticulum-stress-induced-apoptosis-of-trophoblasts-in-intrahepatic-cholestasis-during-pregnancy
#19
Hai-Zhen Wang, Dan-Chun Cai, Dan-Dan Liao, Mei Zhong, Yun-Fei Gao, Chao Sheng
OBJECTIVE: To investigate the role of endoplasmic reticulum stress (ERS)-induced trophoblast apoptosis in the development of intrahepatic cholestasis during pregnancy (ICP). METHODS: Twenty pregnant women with ICP and 20 normal pregnant women undergoing cesarean section were enrolled in this study. The number of placenta syncytial knots in these women was determined using HE staining. The mRNA expressions of GRP78, CHOP, caspase-3, and caspase-7 were detected using RT-PCR in the placental tissues of the women and also in HTR-8/SVneo cells treated with different doses of deoxycholic acid (DCA)...
May 20, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/29887485/first-line-hysterectomy-for-women-with-low-risk-non-metastatic-gestational-trophoblastic-neoplasia-no-longer-wishing-to-conceive
#20
Pierre-Adrien Bolze, Mélodie Mathe, Touria Hajri, Benoit You, Yohann Dabi, Anne-Marie Schott, Sophie Patrier, Jérôme Massardier, François Golfier
BACKGROUND: Low-risk gestational trophoblastic neoplasia (GTN) patients (FIGO score ≤6) are generally treated with single agent chemotherapy (methotrexate or dactinomycin) resulting in a 5-year mortality rate of 0.3%. However, despite these encouraging survival rates, chemotherapy is associated with significant adverse events in most patients. Although it is generally accepted that patients who no longer wish to conceive may be treated by hysterectomy for a hydatidiform mole, the evidence to support this strategy in low-risk GTN patients is lacking...
June 8, 2018: Gynecologic Oncology
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