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Nanovesicles

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https://www.readbyqxmd.com/read/29235485/quantification-of-interacting-cognate-odorants-with-olfactory-receptors-in-nanovesicles
#1
Marta Sanmartí-Espinal, Patrizia Iavicoli, Annalisa Calò, Marta Taulés, Roger Galve, M Pilar Marco, Josep Samitier
This study aims to improve our understanding of the interaction between olfactory receptors and odorants to develop highly selective biosensing devices. Natural nanovesicles (NVs) from Saccharomyces cerevisiae, ~100 nm in diameter, carrying either the human OR17-40 or the chimpanzee OR7D4 olfactory receptor (OR) tagged with the c-myc epitope at their N-terminus, are presented as model systems to quantify the interaction between odorant and olfactory receptors. The level of expression of olfactory receptors was determined at individual NVs using a novel competitive ELISA immunoassay comparing the values obtained against those from techniques involving the solubilization of cell membrane proteins and the identification of c-myc-carrying receptors...
December 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29223657/the-evolutionary-field-hypothesis-non-mendelian-transgenerational-inheritance-mediates-diversification-and-evolution
#2
REVIEW
Corrado Spadafora
Epigenetics is increasingly regarded as a potential contributing factor to evolution. Building on apparently unrelated results, here I propose that RNA-containing nanovesicles, predominantly small regulatory RNAs, are released from somatic tissues in the bloodstream, cross the Weismann barrier, reach the epididymis, and are eventually taken up by spermatozoa; henceforth the information is delivered to oocytes at fertilization. In the model, a LINE-1-encoded reverse transcriptase activity, present in spermatozoa and early embryos, plays a key role in amplifying and propagating these RNAs as extrachromosomal structures...
December 6, 2017: Progress in Biophysics and Molecular Biology
https://www.readbyqxmd.com/read/29217761/aptamer-conjugated-extracellular-nanovesicles-for-targeted-drug-delivery
#3
Yuan Wan, Lixue Wang, Chuandong Zhu, Qin Zheng, Guoxiang Wang, Jinlong Tong, Yuan Fang, Yiqiu Xia, Gong Cheng, Xia He, Si-Yang Zheng
Extracellular nanovesicles (ENV) released by many cells contain lipids, proteins and nucleic acids that contribute to intercellular communication. ENV have emerged as biomarkers and therapeutic targets but they have also been explored as drug delivery vehicles. However, for the latter application clinical translation has been limited by low yield and inadequate targeting effects. ENV vectors with desired targeting properties can be produced from parental cells engineered to express membrane-bound targeting ligands, or they can be generated by fusion with targeting liposomes, however, neither approach has met clinical requirements...
December 7, 2017: Cancer Research
https://www.readbyqxmd.com/read/29216513/a-novel-ultradeformable-liposomes-of-naringin-for-anti-inflammatory-therapy
#4
María Pleguezuelos-Villa, Silvia Mir-Palomo, Octavio Díez-Sales, M A Ofelia Vila Buso, Amparo Ruiz Sauri, Amparo Nácher
Ultradeformable liposomes were formulated using naringin (NA), a flavanone glycoside, at different concentrations (3, 6 and 9mg/mL). Nanovesicles were small size (∼100nm), regardless of the NA concentration used, and monodisperse (PI<0.30). All formulations showed a high entrapment efficiency (∼88%) and a highly negative zeta potential (around -30mV). The selected formulations were highly biocompatible as confirmed by in vitro studies using 3T3 fibroblasts. In vitro assay showed that the amounts (%) of NA accumulated in the epidermis (∼10%) could explain the anti-inflammatory properties of ultradeformable liposomes...
December 1, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29197582/label-free-quantitative-proteomic-profiling-of-colon-cancer-cells-identifies-acetyl-coa-carboxylase-alpha-as-antitumor-target-of-citrus-limon-derived-nanovesicles
#5
Stefania Raimondo, Laura Saieva, Marta Cristaldi, Francesca Monteleone, Simona Fontana, Riccardo Alessandro
We have previously isolated exosome-like nanoparticles from Citrus-limon juice, able to inhibit in vitro and in vivo tumor cell growth. In order to deeply understand the mechanism underlying nanovesicle effects, we performed a proteomic profile of treated colorectal cancer cells. Among the proteins differentially expressed after nanovesicle treatment, we found a significant downregulation of the Acetyl-CoA Carboxylase 1 (ACACA) and we demonstrated that silencing ACACA in cancer cells leads to a reduction of cell growth...
November 29, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29197000/proteomics-method-to-identification-of-protein-profiles-in-exosomes
#6
Andrew Lai, Vyjayanthi Kinhal, Zarin Nuzhat, Ramkumar Menon, Gregory E Rice, Carlos Salomon
Exosomes are membrane-bound nanovesicles that transport molecular signals (e.g., proteins) between cells and are released from a wide range of cells, including the human placenta. Interestingly, the levels of exosomes present in maternal circulation are higher in preeclamptic pregnancies and their protein content profile change in response to the microenvironment milieu. Through the discovery of candidate biomarkers, mass spectrometry (MS)-based proteomics may provide a better understanding of the pathophysiology underlying pregnancy-associated disorders...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29180759/nano-enabled-pancreas-cancer-immunotherapy-using-immunogenic-cell-death-and-reversing-immunosuppression
#7
Jianqin Lu, Xiangsheng Liu, Yu-Pei Liao, Felix Salazar, Bingbing Sun, Wen Jiang, Chong Hyun Chang, Jinhong Jiang, Xiang Wang, Anna M Wu, Huan Meng, Andre E Nel
While chemotherapy delivery by nanocarriers has modestly improved the survival prospects of pancreatic ductal adenocarcinoma (PDAC), additional engagement of the immune response could be game changing. We demonstrate a nano-enabled approach for accomplishing robust anti-PDAC immunity in syngeneic mice through the induction of immunogenic cell death (ICD) as well as interfering in the immunosuppressive indoleamine 2,3-dioxygenase (IDO) pathway. This is accomplished by conjugating the IDO inhibitor, indoximod (IND), to a phospholipid that allows prodrug self-assembly into nanovesicles or incorporation into a lipid bilayer that encapsulates mesoporous silica nanoparticles (MSNP)...
November 27, 2017: Nature Communications
https://www.readbyqxmd.com/read/29175307/extracellular-vesicles-in-obesity-and-diabetes-mellitus
#8
REVIEW
Fabián Pardo, Roberto Villalobos-Labra, Bastián Sobrevia, Fernando Toledo, Luis Sobrevia
Cell-to-cell communication happens via diverse mechanisms including the synthesis, release and transfer to target cells of extracellular vesicles (EVs). EVs include nanovesicles (i.e., exosomes) and microvesicles, including apoptotic bodies. The amount and cargo of released EVs, which consist of microRNAs (miRNAs), mRNA, proteins, DNA, among other molecules, are altered in obesity and diabetes mellitus. EVs from these diseases show with altered cargo including several miRNAs and the enrichment with molecules involved in inflammation, immune efficiency, and cell activation...
November 24, 2017: Molecular Aspects of Medicine
https://www.readbyqxmd.com/read/29172449/exoplexs-chimeric-drug-delivery-platform-from-the-fusion-of-cell-derived-nanovesicles-and-liposomes
#9
Wei Jiang Goh, Shui Zou, Choon Keong Lee, Yi-Hsuan Ou, Jiong-Wei Wang, Bertrand Czarny, Giorgia Pastorin
Cell Derived Nanovesicles (CDNs) have been recently investigated as novel Drug Delivery Systems (DDS), due to the preservation of key features from the cell membrane of their precursor cells, which are responsible for an efficient cellular uptake by target cells. However, CDNs suffer from low drug loading efficiencies as well as challenges in functionalization compared to conventional DDS like liposomes. Here, we describe the first study proposing the fusion of CDNs with liposomes to form EXOPLEXs. We report the preservation of cell membranes from precursor cells similarly to CDNs, as well as high loading efficiencies of more than 65% with doxorubicin hydrochloride, a model chemotherapeutic drug...
November 27, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/29158713/formulation-and-characterization-of-novel-soft-nanovesicles-for-enhanced-transdermal-delivery-of-eprosartan-mesylate
#10
Abdul Ahad, Abdulmohsen A Al-Saleh, Abdullah M Al-Mohizea, Fahad I Al-Jenoobi, Mohammad Raish, Alaa Eldeen B Yassin, Mohd Aftab Alam
The objective of the present work was to formulate, optimize and evaluate the potential of novel soft nanovesicles i.e. nano-transfersomes, containing eprosartan mesylate (EM) for transdermal delivery. Nano-transfersomes of EM were developed using Phospholipon 90G, Span 80 (SP) and sodium deoxycholate (SDC) and characterized for vesicle size, shape, entrapment efficiency, in vitro skin permeation study and confocal laser scanning microscopy. The optimized nano-transfersomes formulation showed vesicles size of 108...
November 2017: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://www.readbyqxmd.com/read/29155074/photodynamic-diagnosis-of-parathyroid-glands-with-nano-stealth-aminolevulinic-acid-liposomes
#11
Shaimaa Elbassiouny, Maha Fadel, Tarek Elwakeel, Mahmoud Saber
AIM OF THE STUDY: Exploring whether the formulation of ALA in nano-stealth liposomes would inflict its photodiagnostic effectiveness. The present study represents ALA in nano-stealth liposomes as well as in conventional nanoliposomes in order to be used for photodiagnosis of parathyroid glands intraoperatively. MATERIALS AND METHODS: Preparation of ALA nanovesicles and characterization for the drug encapsulation%, vesicle size, Zeta potential, in vitro drug release profile and stability...
November 15, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/29148794/preparation-and-formation-process-of-zn-ii-coordinated-nanovesicles
#12
Wuxiao Ding, Naohiro Kameta, Hiroyuki Minamikawa, Mitsutoshi Masuda, Masaki Kogiso
Mixing a glycylglycine-lipid and zinc acetate has been reported to form novel supramolecular Zn(II)-coordinated nanovesicles in ethanol. In this study, we investigate in detail the formation of nanovesicles by using three lipids at different temperatures and discuss their formation process. The original lipids show extremely low solubilities and appear as plate structures in ethanol. Within a small window of lipid solubility, the formation of lipid-Zn(II) complexes occurs mainly on the solid surfaces of plate structures...
November 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29147558/discovery-and-design-of-self-assembling-peptides
#13
REVIEW
Shuguang Zhang
Peptides are ubiquitous in nature and useful in many fields, from agriculture as pesticides, in medicine as antibacterial and antifungal drugs founded in the innate immune systems, to medicinal chemistry as hormones. However, the concept of peptides as materials was not recognized until 1990 when a self-assembling peptide as a repeating segment in a yeast protein was serendipitously discovered. Peptide materials are so called because they have bona fide materials property and are made from simple amino acids with well-ordered nanostructures under physiological conditions...
December 6, 2017: Interface Focus
https://www.readbyqxmd.com/read/29145622/plant-extracellular-vesicles-are-incorporated-by-a-fungal-pathogen-and-inhibit-its-growth
#14
Mariana Regente, Marcela Pinedo, Hélène San Clemente, Thierry Balliau, Elisabeth Jamet, Laura de la Canal
Extracellular vesicles (EV) are membrane particles released by cells into their environment and are considered to be key players in intercellular communication. EV are produced by all domains of life but limited knowledge about EV in plants is available, although their implication in plant defense has been suggested. We have characterized sunflower EV and tested whether they could interact with fungal cells. EV were isolated from extracellular fluids of seedlings and characterized by transmission electron microscopy and proteomic analysis...
November 14, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/29133980/tailored-nanostructured-platforms-for-boosting-transcorneal-permeation-box-behnken-statistical-optimization-comprehensive-in-vitro-ex-vivo-and-in-vivo-characterization
#15
Ibrahim Elsayed, Sinar Sayed
Ocular drug delivery systems suffer from rapid drainage, intractable corneal permeation and short dosing intervals. Transcorneal drug permeation could increase the drug availability and efficiency in the aqueous humor. The aim of this study was to develop and optimize nanostructured formulations to provide accurate doses, long contact time and enhanced drug permeation. Nanovesicles were designed based on Box-Behnken model and prepared using the thin film hydration technique. The formed nanodispersions were evaluated by measuring the particle size, polydispersity index, zeta potential, entrapment efficiency and gelation temperature...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29128664/intravenous-anti-mrsa-phosphatiosomes-mediate-enhanced-affinity-to-pulmonary-surfactants-for-effective-treatment-of-infectious-pneumonia
#16
Ching-Yun Hsu, Calvin T Sung, Ibrahim A Aljuffali, Chun-Han Chen, Kai-Yin Hu, Jia-You Fang
The aim of this study was to develop PEGylated phosphatidylcholine (PC)-rich nanovesicles (phosphatiosomes) carrying ciprofloxacin (CIPX) for lung targeting to eradicate extracellular and intracellular methicillin-resistant Staphylococcus aureus (MRSA). Soyaethyl morphonium ethosulfate (SME) was intercalated in the nanovesicle surface with the dual goals of achieving strengthened bactericidal activity of CIPX-loaded phosphatiosomes and delivery to the lungs. The isothermal titration calorimetry (ITC) results proved the strong association of SME phosphatiosomes with pulmonary surfactant...
November 8, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29127888/histamine-stimulates-secretion-of-extracellular-vesicles-with-nucleotidase-activity-in-rat-submandibular-gland
#17
Débora Alejandra González, Martín Matías Barbieri van Haaster, Emmanuel Quinteros Villarruel, Macarena Brandt, María Belén Benítez, Graciela Mabel Stranieri, Betina Orman
BACKGROUND: Extracellular vesicles released by different cells have been isolated from diverse fluids including saliva. We previously reported that rat submandibular glands secrete nanovesicles that catalyze hydrolysis of ATP, ADP and AMP, which are actors of the purinergic signaling system along with adenosine. Extracellular nucleotides like ATP and adenosine are involved in the regulation of inflammatory processes and apoptosis. Histamine, a widely distributed biogenic amine, is involved in inflammatory response...
October 18, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29126906/development-and-characterization-of-an-organic-solvent-free-proliposomal-formulation-of-busulfan-using-quality-by-design-approach
#18
Dhawal Chobisa, Ketan Patel, Jasmin Monpara, Mayank Patel, Pradeep Vavia
Parenteral administration of Busulfan (BU) conquers the bioavailability and biovariability related issues of oral BU by maintaining the plasma drug concentration in therapeutic range with minimal fluctuations thereby significantly reducing the side effects. Busulfex(®) is the only commercially available parenteral formulation of BU composed of organic solvents N, N-dimethylacetamide and polyethylene glycol 400. Since, BU is highly susceptible to hydrolytic degradation; Busulfex(®) has poor physical and chemical stability in IV fluids...
November 7, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29125180/exosomes-biology-therapeutic-potential-and-emerging-role-in-musculoskeletal-repair-and-regeneration
#19
REVIEW
Neil J Cobelli, Daniel J Leong, Hui B Sun
Exosomes are nanovesicles secreted from cells that play key roles in intercellular communication. They carry unique content derived from parental cells and are capable of transferring this cargo between cells. The role and function of exosomes largely depends on the origin and functional status of the parental cells. Emerging evidence indicates that exosomes are associated with biological processes and pathogenesis of certain diseases. These nanovesicles offer great potential as biomarkers, enabling the monitoring and diagnosis of various diseases in a noninvasive manner...
November 10, 2017: Annals of the New York Academy of Sciences
https://www.readbyqxmd.com/read/29111329/exosomes-derived-from-pancreatic-cancer-cells-induce-activation-and-profibrogenic-activities-in-pancreatic-stellate-cells
#20
Atsushi Masamune, Naoki Yoshida, Shin Hamada, Tetsuya Takikawa, Tatsuhide Nabeshima, Tooru Shimosegawa
Pancreatic cancer cells (PCCs) interact with pancreatic stellate cells (PSCs), which play a pivotal role in pancreatic fibrogenesis, to develop the cancer-conditioned tumor microenvironment. Exosomes are membrane-enclosed nanovesicles, and have been increasingly recognized as important mediators of cell-to-cell communications. The aim of this study was to clarify the effects of PCC-derived exosomes on cell functions in PSCs. Exosomes were isolated from the conditioned medium of Panc-1 and SUIT-2 PCCs. Human primary PSCs were treated with PCC-derived exosomes...
October 27, 2017: Biochemical and Biophysical Research Communications
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