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https://www.readbyqxmd.com/read/28932842/microfluidic-isolation-of-platelet-covered-circulating-tumor-cells
#1
Xiaocheng Jiang, Keith H K Wong, Aimal H Khankhel, Mahnaz Zeinali, Eduardo Reategui, Matthew J Phillips, Xi Luo, Nicola Aceto, Fabio Fachin, Anh N Hoang, Wooseok Kim, Annie E Jensen, Lecia V Sequist, Shyamala Maheswaran, Daniel A Haber, Shannon L Stott, Mehmet Toner
The interplay between platelets and tumor cells is known to play important roles in metastasis by enhancing tumor cell survival, tumor-vascular interactions, and escape from immune surveillance. However, platelet-covered circulating tumor cells (CTC) are extremely difficult to isolate due to masking or downregulation of surface epitopes. Here we describe a microfluidic platform that takes advantage of the satellite platelets on the surface of these "stealth" CTCs as a ubiquitous surface marker for isolation...
September 21, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28929739/ultrasensitive-capture-detection-and-release-of-circulating-tumor-cells-using-nanochannel-ionchannel-hybrid-coupled-with-electrochemical-detection-technique
#2
Jing Cao, Xiao-Ping Zhao, Muhammad Rizwan Younis, Zhong-Qiu Li, Xing-Hua Xia, Chen Wang
With the growing demands of the early, accurate, and sensitive diagnosis for cancers, the development of new diagnostic technologies becomes increasingly important. In this study, we proposed a strategy for efficient capture and sensitive detection of circulating tumor cells (CTCs) using array nanochannel-ionchannel hybrid coupled with electrochemical detection technique. The aptamer probe was immobilized on the ionchannel surface to couple with the protein overexpressed on CTCs membrane. Through this special molecular recognition, CTCs can be selectively captured...
September 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28928891/overexpression-of-lamc1-predicts-poor-prognosis-and-enhances-tumor-cell-invasion-and-migration-in-hepatocellular-carcinoma
#3
Yaojun Zhang, Shaoyan Xi, Jinbin Chen, Dongsheng Zhou, Hengjun Gao, Zhongguo Zhou, Li Xu, Minshan Chen
LAMC1 encodes an extracellular matrix protein, laminin γ1 chain, which is involved in several biological and pathological processes including tissue development, tumor cell invasion and metastasis. In present study, we demonstrated that both LAMC1 protein and mRNA levels were elevated in HCC tissue samples compared with non-cancerous tissue samples according to western blot analyses, immunohistochemistry (IHC) and microarray. Moreover, high LAMC1 expression was positively correlated with incomplete encapsulation (p=0...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/28928445/phase-retrieved-tomography-enables-mesoscopic-imaging-of-opaque-tumor-spheroids
#4
Daniele Ancora, Diego Di Battista, Georgia Giasafaki, Stylianos E Psycharakis, Evangelos Liapis, Jorge Ripoll, Giannis Zacharakis
We present a new Phase-Retrieved Tomography (PRT) method to radically improve mesoscopic imaging at regimes beyond one transport mean-free-path and achieve high resolution, uniformly throughout the volume of opaque samples. The method exploits multi-view acquisition in a hybrid Selective Plane Illumination Microscope (SPIM) and Optical Projection Tomography (OPT) setup and a three-dimensional Gerchberg-Saxton phase-retrieval algorithm applied in 3D through the autocorrelation sinogram. We have successfully applied this innovative protocol to image optically dense 3D cell cultures in the form of tumor spheroids, highly versatile models to study cancer behavior and response to chemotherapy...
September 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28921173/maximizing-safe-resections-the-roles-of-5-aminolevulinic-acid-and-intraoperative-mr-imaging-in-glioma-surgery-review-of-the-literature
#5
REVIEW
Eric Suero Molina, S Schipmann, W Stummer
Malignant glioma surgery involves the challenge of preserving the neurological status of patients harboring these lesions while pursuing a maximal tumor resection, which is correlated with overall and progression-free survival. Presently, several tools exist for assisting neurosurgeons in visualizing malignant tissue. Fluorescence-guided surgery (FGS) with 5-aminolevulinic acid (5-ALA) has increasingly been used during the last decade for identifying malignant glioma. Intraoperative magnetic resonance imaging (iMRI), first introduced in the mid-1990s, is being evaluated as a further tool to maximize the extent of resection...
September 18, 2017: Neurosurgical Review
https://www.readbyqxmd.com/read/28920163/correction-of-whistle-deformity-using-autologous-free-fat-grafting-first-results-of-a-pilot-study-and-review-of-the-literature
#6
Sven Holger Baum, Gunnar Rieger, Roman Pförtner, Christopher Mohr
PURPOSE: Whistle deformities are frequent sequelae after surgical correction of cleft lip, trauma, or tumor excision. The aim of this study was to examine the role of autologous free fat grafting in the reconstruction of whistle deformity. PATIENTS: Fifteen patients with whistle deformity were enrolled in this pilot study. The mean follow-up period was 19 months. Liposuction was done followed by the replantation of an average of 2.2 ml autologous fat per patient (range 0...
September 17, 2017: Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28917012/operative-and-short-term-oncologic-outcomes-of-laparoscopic-versus-open-liver-resection-for-colorectal-liver-metastases-located-in-the-posterosuperior-liver-a-propensity-score-matching-analysis
#7
Masayuki Okuno, Claire Goumard, Takashi Mizuno, Kiyohiko Omichi, Ching-Wei D Tzeng, Yun Shin Chun, Thomas A Aloia, Jason B Fleming, Jeffrey E Lee, Jean-Nicolas Vauthey, Claudius Conrad
BACKGROUND: Laparoscopic resection (LLR) of colorectal liver metastases (CRLM) located in the posterosuperior liver (segments 4a, 7, and 8) is challenging but has become more practical recently due to progress in operative techniques. We aimed to compare tumor-specific, perioperative, and short-term oncological outcomes after LLR and open liver resection (OLR) for CRLM. METHODS: Patients who underwent curative resection of CRLM with at least 1 tumor in the posterosuperior liver during 2012-2015 were analyzed...
September 15, 2017: Surgical Endoscopy
https://www.readbyqxmd.com/read/28916260/primary-facial-reanimation-in-head-and-neck-cancer
#8
REVIEW
Axel Sahovaler, David Yeh, John Yoo
Facial Paralysis (FP) profoundly impairs the life of individuals, both functionally and psychosocially. Surgical approaches to treat this condition are myriad, but the ultimate goal is to restore symmetry and movement. Ablative surgery for tumors of the head and neck region are amongst the most common etiologies causing FP and this group of patients represents unique challenges. Surgical defects may have multiple competing reconstructive requirements and addressing the FP must be considered in this context...
September 12, 2017: Oral Oncology
https://www.readbyqxmd.com/read/28912145/dynamic-18-f-fdg-pet-lymphography-for-in-vivo-identification-of-lymph-node-metastases-in-murine-melanoma
#9
Hannah H Lockau, Volker Neuschmelting, Anuja Ogirala, Antoni Vilaseca, Jan Grimm
Objective: Positron Lymphography using (18)F-FDG followed by Cerenkov guided resection of lymph nodes in healthy mice has previously been introduced by our group. Our aim in this study was to further assess the technique`s potential beyond merely localizing sentinel lymph nodes. We now aimed to evaluate the potential of Positron Lymphography to characterize the nodes with respect to their tumor status on order to identify metastatic lymph nodes. We explored if metastatic nodes could be distinguished from normal nodes via dynamic (18)F-FDG-lymphography, in order to then be resected under Cerenkov imaging guidance...
September 14, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/28910208/immunohistochemical-and-immunocytochemical-findings-associated-with-marek-s-disease-virus-in-naturally-infected-laying-hens
#10
O Yavuz, H Erer
We compared immunohistochemical (IHC) staining of tissue sections of liver, kidney, spleen, lung, proventriculus, sciatic nerve, bursa of Fabricius, brain, heart, intestine and skin; immunocytochemical (ICC) staining of peripheral blood samples and touch preparations of liver, spleen and kidney of laying hens naturally infected with Marek's disease (MD) virus. We used one hundred and fifty 5-17-week-old commercial hens. IHC and ICC staining were performed using polymer-based techniques. IHC staining exhibited mostly free immunopositive reactions in tumor cells and in the cytoplasm of the parenchymal cells of liver, kidney, spleen and bursa of Fabricius...
September 14, 2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/28900598/cationic-pegylated-polycaprolactone-nanoparticles-carrying-post-operation-docetaxel-for-glioma-treatment
#11
Cem Varan, Erem Bilensoy
Background: Brain tumors are the most common tumors among adolescents. Although some chemotherapeutics are known to be effective against brain tumors based on cell culture studies, the same effect is not observed in clinical trials. For this reason, the development of drug delivery systems is important to treat brain tumors and prevent tumor recurrence. The aim of this study was to develop core-shell polymeric nanoparticles with positive charge by employing a chitosan coating. Additionally, an implantable formulation for the chemotherapeutic nanoparticles was developed as a bioadhesive film to be applied at the tumor site following surgical operation for brain glioma treatment...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28898798/free-disease-long-term-survival-in-primary-thoracic-spine-leiomyosarcoma-after-total-en-bloc-spondylectomy-a-case-report
#12
José Ramirez-Villaescusa, Adriana Canosa-Fernández, Antonio Martin-Benlloch, David Ruiz-Picazo, Jesús López-Torres Hidalgo
INTRODUCTION: To describe an unusual primary vertebral leiomyosarcoma in thoracic spine. PRESENTATION OF CASE: An isolated lesion of the T11 vertebra in a 62-year-old woman with no neurologic deficit is reported. Imaging findings indicated a nonspecific high-grade malignant lesion. TC-guided biopsy failed thus open incisional biopsy was needed. A diagnosis of low-intermediate mesenchymal sarcoma was made. A total en bloc spondylectomy of T11 was performed with three-column reconstruction...
September 1, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/28889390/dna-methylation-analysis-from-body-fluids
#13
Dimo Dietrich
Circulating cell-free DNA (ccfDNA) can be found in various body fluids, i.e., blood (serum and plasma), urine, pleural effusions, and ascites. While ccfDNA predominantly originates from physiological processes, a fraction might be related to pathological events, e.g., cancer. Aberrant DNA methylation, which is considered a hallmark of cancer, can be assessed accurately in ccfDNA. Consequently, DNA methylation testing in body fluids represents a powerful diagnostic tool in the clinical management of malignant diseases...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28885214/breast-sensitivity-after-mastectomy-and-autologous-reconstruction
#14
Norbert Heine, Christoph Koch, Vanessa Brebant, Andreas Kehrer, Alexandra Anker, Lukas Prantl
BACKGROUND: Breast Carcinoma is the most common malign tumor disease in women. 20-30% of these tumors have to be treated by removing all of the breast tissue. After that kind of therapeutic procedure, most of the women are severely traumatized and ask for reconstruction. The goldstandard of breast reconstruction is the free perforator flap from the lower abdomen (DIEAP-flap), which can provide body-own tissue and natural shape for the reconstructed breast.Many studies evaluate the shape of the reconstructed breast but neglect the skin sensitivity...
September 4, 2017: Clinical Hemorheology and Microcirculation
https://www.readbyqxmd.com/read/28881740/unit-resection-of-buccal-squamous-cell-carcinoma-description-of-a-new-surgical-technique
#15
Zhen-Hu Ren, Zhao-Jian Gong, Han-Jiang Wu
This study characterized the infiltration of primary tumors along the muscles, fascia and spaces of the maxillofacial region in buccal squamous cell carcinoma (BSCC) and suggested a new surgical strategy that is suitable for most stages. Based on the anatomic characteristics and infiltration of the primary tumor a new surgical approach - unit resection buccal surgery (URBS) - was developed. We evaluated this new surgical strategy, across a cohort of 127 BSCCs: 60 cases treated with URBS and 67 cases treated with conventional surgery...
August 8, 2017: Oncotarget
https://www.readbyqxmd.com/read/28878541/an-unusual-localization-of-chondroblastoma-the-triradiate-cartilage-from-a-case-report-a-reconstructive-technique-proposal-with-imaging-evolution
#16
Carmine Zoccali, Francesco Arrigoni, Silvia Mariani, Federico Bruno, Antonio Barile, Carlo Masciocchi
INTRODUCTION: Chondroblastoma (also known as Codman tumor) is a rare intermediate grade cartilaginous neoplasm, representing less than 1% of all primary bone tumors; it characteristically arises in the epiphysis or apophysis of a long bone in young patients, predominantly males. The most frequent location of chondroblastoma is the humerus (70% incidence rate) and more rarely it is located in the pelvis. When it affects the hip, the triradiate cartilage is the most common site. MATERIALS AND METHODS: An unusual case of Chondroblastoma located in the triradiate cartilage is reported...
August 2017: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/28875398/total-hilar-en-bloc-resection-with-left-hemihepatectomy-and-caudate-lobectomy-a-novel-approach-for-treatment-of-left-sided-perihilar-cholangiocarcinoma-with-video
#17
Cai De Lu, Jing Huang, Sheng Dong Wu, Yong Fei Hua, Ammar A Javed, Jiong Zhe Fang, Chun Nian Wang, Sheng Ye
BACKGROUND: "Hilar en bloc resection" using a no-touch technique has been advocated as a standard procedure in right-sided hepatectomies for treatment of perihilar cholangiocarcinoma (PHC). In principle, it has never been reported for left-sided tumors. The aim is to describe the procedures of total hilar en bloc resection with left hemihepatectomy and caudate lobectomy (THER-LH) for advanced PHC and discuss feasibility and clinical significance of this novel technique. METHODS: A retrospective study using a prospectively maintained database was performed to identify eight patients who had received THER-LH for advanced PHC from January 2013 to December 2015...
September 5, 2017: Journal of Gastrointestinal Surgery: Official Journal of the Society for Surgery of the Alimentary Tract
https://www.readbyqxmd.com/read/28868287/intracameral-chemotherapy-melphalan-for-aqueous-seeding-in-retinoblastoma-bicameral-injection-technique-and-related-toxicity-in-a-pilot-case-study
#18
Francis L Munier, Marie-Claire Gaillard, Sarah Decembrini, Massimo Bongiovanni, Maja Beck-Popovic
BACKGROUND: The anterior chamber has been shown by pharmacokinetic studies to represent a sanctuary never achieving a tumoricidal dose with the present administration routes, such as systemic, intra-arterial, or intravitreal injections. METHOD: A novel intracameral chemotherapy technique is described to control aqueous seeding in a pilot unilateral group E retinoblastoma case with primary aqueous seeding. Anterior segment toxicity was carefully monitored. RESULTS: Control of the retinal tumor and vitreous seeding was achieved by intra-arterial and intravitreal chemotherapies...
July 2017: Ocular Oncology and Pathology
https://www.readbyqxmd.com/read/28868131/circulating-tumor-cells-silent-predictors-of-metastasis
#19
REVIEW
LanLan Zhou, David T Dicker, Elizabeth Matthew, Wafik S El-Deiry, R Katherine Alpaugh
Circulating tumor cells (CTCs) were added to the arsenal of clinical testing in 2004 for three cancer types: metastatic breast, prostate, and colorectal cancer. CTCs were found to be an independent prognostic indicator of survival for these three diseases. Multiple enrichment/isolation strategies have been developed and numerous assay applications have been performed using both single and pooled captured/enriched CTCs. We have reviewed the isolation techniques and touched on many analyses. The true utility of a CTC is that it acts as a "silent" predictor of metastatic disease...
2017: F1000Research
https://www.readbyqxmd.com/read/28863309/polymeric-nanoparticles-of-sirna-prepared-by-a-double-emulsion-solvent-diffusion-technique-physicochemical-properties-toxicity-biodistribution-and-efficacy-in-a-mammary-carcinoma-mice-model
#20
Meital Ben David-Naim, Etty Grad, Gil Aizik, Mirjam M Nordling-David, Ofra Moshel, Zvi Granot, Gershon Golomb
siRNA-loaded nanoparticles (NPs) administered systemically can overcome the poor stability and rapid elimination of free double-stranded RNA in circulation, resulting in increased tumor accumulation and efficacy. siRNA against osteopontin (siOPN), a protein involved in breast cancer development, was encapsulated in poly(D,L-lactic-co-glycolic acid) NPs by a double emulsion solvent diffusion (DESD) technique. We also compared the effect of polyethylenimine (PEI) molecular weight (800 Da and 25 kDa), used as the counter-ion for siRNA complexation, on the physicochemical properties of the NPs, cytotoxicity, and cellular uptake...
August 23, 2017: Biomaterials
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