Read by QxMD icon Read


Jianhua Ren, Yadwinder S Mann, Yuntao Zhang, Michael D Browne
Peptoids are sequence-controlled peptide-mimicking oligomers consisting of N-alkylated glycine units. Among many potential applications, peptoids have been thought of as a type of molecular information storage. Mass spectrometry analysis has been considered the method of choice for sequencing peptoids. Peptoids can be synthesized via solid phase chemistry using a repeating two-step reaction cycle. Here we present a method to manually synthesize oligo-peptoids and to analyze the sequence of the peptoids using tandem mass spectrometry (MS/MS) techniques...
February 21, 2018: Journal of Visualized Experiments: JoVE
Yuan-Sheng Lee, Nirveek Bhattacharjee, Albert Folch
Here we demonstrate a 3D-printable microvalve that is transparent, built with a biocompatible resin, and has a simple architecture that can be easily scaled up into large arrays. The open-at-rest valve design is derived from Quake's PDMS valve design. We used a stereolithographic (SL) 3D printer to print a thin (25 or 10 μm-thick) membrane (1200 or 500 μm-diam.) that is pneumatically pressed (∼3-6 psi) over a bowl-shaped seat to close the valve. We used poly(ethylene diacrylate) (MW = 258) (PEG-DA-258) as the resin because it yields transparent cytocompatible prints...
March 19, 2018: Lab on a Chip
Sufyan Garoushi, Pekka K Vallittu, Lippo Lassila
This study investigated the reinforcing effect of discontinuous-glass fiber fillers with different loading-fractions on selected mechanical properties and wear of resin-modified glass ionomer cement (RMGIC). Experimental fiber-reinforced RMGIC (Exp-RMGIC) was prepared by adding discontinuous-glass fiber of 200-500 µm in length to the powder of RMGIC (GC Fuji II LC) with different weight ratios (15, 20, 25 and 30 wt%). Mechanical properties and wear were determined for each experimental and control material...
March 17, 2018: Dental Materials Journal
Hiroyasu Kurokawa, Koji Shiratsuchi, Shunichi Suda, Yuko Nagura, Soshi Suzuki, Nobuyuki Moritake, Kabun Yamauchi, Masashi Miyazaki
In this study, we investigated the effect of light irradiation and primer/adhesive application on the polymerization reaction and elastic modulus (E) of self-adhesive resin cement (SAC) through ultrasonic velocity (V) measurements. The V values of longitudinal and shear waves were determined based on the transit time through the specimens and specimen thicknesses, and were used to calculate the E values. Analysis of variance and Tukey tests revealed that V increased rapidly, reaching a plateau at 2,700-2,900 m/s, when the SACs were light-irradiated...
March 17, 2018: Dental Materials Journal
Yuling Xu, Juntao Zhang, Haibo Wang, Dong Xie
Several new urethane-based dimethacrylates were synthesized, characterized and used to formulate the resin composites. Compressive strength (CS) was used as a screen tool to evaluate the mechanical property of the formed composites. Flexural strength, diametral tensile strength, water sorption, degree of conversion and shrinkage of the composites were also evaluated. The results show that most of the synthesized urethane-based dimethacrylates were solid, which are not suitable to dental filling restorations...
March 17, 2018: Dental Materials Journal
Feng-Hua Li, Hong-Qing Wang, Xian-Ming Su, Chang-Kang Li, Bao-Ming Li, Ruo-Yun Chen, Jie Kang
Seventeen compounds were isolated from n-butanol extract of the leaves of Moringa oleifera, using column chromatography over macroporous resin HP-20,Sephadex LH-20, and ODS. Their structures were identified as two carboline,tangutorid E(1) and tangutorid F(2); three phenolic glycosides,niazirin(3),benzaldehyde 4-O-α-L-rhamnopyranoside(4) and 4-O-β-D-glucopyranosidebenzoic acid(5); four chlorogenic acid and derivatives,4-caffeoylquinic acid(6),methyl 4-caffeoylquinate(7),caffeoylquinic acid(8) and methyl caffeoylquinate(9); two nucleosids,uridine(10) and adenosine(11); one flavone,quercetin 3-O-β-D-glucopyranoside(12); five other types of compounds,phthalimidineacetic acid(13),3-pyridinecarboxamide(14),3,4-dihydroxy-benzoic acid(15),5-hydroxymethyl-2-furancarboxylic acid(16) and 5-hydroxymethyl-2-furaldehyde(17) by the spectral data of ¹H, ¹³C-NMR and MS...
January 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Sheng Lin, Peng Fu, Yun-Heng Shen, Ji Ye, Zhong-Xiao Zhang, Xian-Wen Yang, Hui-Liang Li, Run-Hui Liu, Xi-Ke Xu, Wei-Dong Zhang
Application of a combination of various chromatographic techniques including column chromatography over silica gel, Sephadex LH-20, macroporous adsorbent resin, and reversed-phase HPLC, led to the isolation of 173 compounds including irdidoids, monoterpenes, sesquiterpenes, triterpenes, lignans, flavonoids, and simple aromatic derivatives from the ethyl acetate-soluble fraction of the whole plants of Valeriana jatamansi(Valerianaceae), and their structures were elucidated by spectroscopic methods including 1D, 2D NMR UV, IR, and MS techniques...
January 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
Ines Kallel, Eya Moussaoui, Fadwa Chtioui, Nabiha Douki
According to the American Association of Endodontists, "a 'true' vertical root fracture is defined as a complete or incomplete fracture initiated from the root at any level, usually directed buccolingually." Vertical root fracture (VRF) usually starts from an internal dentinal crack and develops over time, due to masticatory forces and occlusal loads. When they occur in teeth, those types of fractures can present difficulties in diagnosis, and there are however many clinic and radiographical signs which can guide clinicians to the existence of the fracture...
2018: Case Reports in Dentistry
Sameh S M Soliman, Manish N Raizada
Fungal Taxol acquired lots of attention in the last few decades mainly because of the hope that fungi could be manipulated more easily than yew trees to scale up the production level of this valuable anticancer drug. Several researchers have studied diverse factors to enhance fungal Taxol production. However, up to date fungal Taxol production has never been enhanced to the commercial level. We have hypothesized that optimization of fungal Taxol production may require clear understanding of the fungal habitat in its original host plant...
2018: Frontiers in Microbiology
Fernanda Ferruzzi Lima, Constantino Fernandes Neto, José H Rubo, Gildo Coelho Santos, Maria Jacinta Moraes Coelho Santos
STATEMENT OF PROBLEM: Factors that may affect the marginal adaptation of computer-aided design and computer-aided manufacturing (CAD-CAM) restorations include preparation design, impression technique, and CAD-CAM system. The influence of impression technique and preparation design on CAD-CAM partial coverage restorations has not been fully addressed. PURPOSE: The purpose of this in vitro study was to investigate the influence of direct and indirect digital impression techniques and 2 preparation designs on the marginal adaptation of CAD-CAM onlays...
March 15, 2018: Journal of Prosthetic Dentistry
Won-Suk Oh, Ju-Mi Park
No abstract text is available yet for this article.
March 15, 2018: Journal of Prosthetic Dentistry
Keitaro Kubo, Takashi Koike, Takayuki Ueda, Kaoru Sakurai
STATEMENT OF PROBLEM: Information is lacking about the selection criteria for silicone resilient denture liners applied as a matrix material for attachments on overdentures. PURPOSE: The purpose of this in vitro study was to investigate the mechanical properties of silicone resilient denture liners and their influence on the initial retention force of overdenture attachments and the reduction in retention force over time. MATERIAL AND METHODS: Nine types of silicone resilient denture liner were injected and fixed to the matrix section of an experimental denture base...
March 15, 2018: Journal of Prosthetic Dentistry
Jody L Clements, Daranee Tantbirojn, Antheunis Versluis, David R Cagna
STATEMENT OF PROBLEM: Denture tooth fracture may limit the longevity of dental prostheses. Whether the strength of the denture tooth material is affected by the denture processing technique is unclear. PURPOSE: The purpose of this in vitro study was to investigate whether the denture processing technique affects the mechanical properties of denture tooth materials. MATERIAL AND METHODS: Two denture processing techniques, injection and compression molding, were tested for 3 types of denture teeth: nanohybrid composite (NHC), interpenetrating network (IPN), and microfiller-reinforced polyacrylic (MRP)...
March 15, 2018: Journal of Prosthetic Dentistry
Hamad S AlRumaih, Abdulaziz AlHelal, Nadim Z Baba, Charles J Goodacre, Ali Al-Qahtani
STATEMENT OF PROBLEM: Clinical studies have identified advantages of digital complete denture technology including patient satisfaction, improved mastication, increased retention, and technique efficiency. However, studies that focusing on the effect of denture adhesive on the retention of milled and heat-activated denture bases are lacking. PURPOSE: The purpose of this clinical study was to evaluate the effectiveness of denture adhesive on the retention of milled and heat-activated denture bases...
March 15, 2018: Journal of Prosthetic Dentistry
Arch Creasy, Joseph Lomino, Gregory Barker, Anurag Khetan, Giorgio Carta
Protein retention in hydrophobic interaction chromatography is described by the solvophobic theory as a function of the kosmostropic salt concentration. In general, an increase in salt concentration drives protein partitioning to the hydrophobic surface while a decrease reduces it. In some cases, however, protein retention also increases at low salt concentrations resulting in a U-shaped retention factor curve. During gradient elution the salt concentration is gradually decreased from a high value thereby reducing the retention factor and increasing the protein chromatographic velocity...
March 9, 2018: Journal of Chromatography. A
Atsushi Hirano, Kazuki Iwashita, Shun Sakuraba, Kentaro Shiraki, Tsutomu Arakawa, Tomoshi Kameda
Ion-exchange chromatography and multimodal ion-exchange chromatography are widely used for the separation of small molecules, peptides and proteins. Salts generally attenuate the electrostatic interactions between charged moieties of solutes and those of resins through electrostatic screening. However, little is known about how salts affect the interaction between the uncharged moieties of the solutes, such as aromatic moieties, and the charged moieties of the resins. In this study, we used alkyl gallates as model aromatic solutes to investigate the interaction mechanism of aromatic moieties with multimodal and conventional ion-exchange resins...
March 6, 2018: Journal of Chromatography. A
Justin Weinberg, Shaojie Zhang, Allison Kirkby, Enosh Shachar, Giorgio Carta, Todd Przybycien
We have proposed chemical modification of Protein A (ProA) chromatography ligands with polyethylene glycol (PEGylation) as a strategy to increase the resin selectivity and robustness by providing the ligand with a steric repulsion barrier against non-specific binding. Here, we report on robustness and selectivity benefits for Repligen CaptivA PriMAB resin with ligands modified with 5.2 kDa and 21.5 kDa PEG chains, respectively. PEGylation of ProA ligands allowed the resin to retain a higher percentage of static binding capacity relative to the unmodified resin upon digestion with chymotrypsin, a representative serine protease...
February 24, 2018: Journal of Chromatography. A
Justin Weinberg, Shaojie Zhang, Gillian Crews, Giorgio Carta, Todd Przybycien
Chemical modification of Protein A (ProA) chromatography ligands with polyethylene glycol (PEGylation) has been proposed as a strategy to increase the process selectivity and resin robustness by providing the ligand with a steric repulsion barrier against non-specific binding. This article comprises a comprehensive study of IgG adsorption and transport in Repligen CaptivA PriMAB resin with PEGylated ProA ligands that are modified using 5.2 and 21.5 kDa PEG chains. We studied the impact of the molecular weight of the PEG as well as the extent of PEGylation for the 5...
March 9, 2018: Journal of Chromatography. A
Xueting Liu, Liping Zeng, Zhongqiu Zhao, Yang Xie, Shan Wang, Junyan Zhang, Ying He, Zehong Zou, Jianguo Zhang, Ailin Tao
Staphylococcal enterotoxin A is well known as a superantigen and able to be used for cancer immunotherapy. In this study, recombinant Staphylococcal enterotoxin A was genetically conjugated to epidermal growth factor to produce a chimeric protein recombinant Staphylococcal enterotoxin A-epidermal growth factor expressed in Escherichia coli. The recombinant Staphylococcal enterotoxin A-epidermal growth factor protein was purified using Strep-Tactin affinity chromatography and Endotoxin Removal Resin and identified by sodium dodecyl sulfate-polyacrylamide gel electropheresis and liquid chromatography-tandem mass spectrometry analysis...
January 1, 2018: Technology in Cancer Research & Treatment
Wei Chen, Suhong Pan, Hao Cheng, Andrew J Sweetman, Hao Zhang, Kevin C Jones
A passive water sampler based on the diffusive gradients in thin-films (DGT) technique was developed and tested for 3 groups of endocrine disrupting chemicals (EDCs, including oestrogens, alkyl-phenols and bisphenols). Three different resins (hydrophilic-lipophilic-balanced (HLB), XAD18 and Strata-XL-A (SXLA)) were investigated for their suitability as the binding phase for DGT devices. Laboratory tests across a range of pH (3.5-9.5), ionic strength (0.001-0.5 M) and dissolved organic matter concentration (0-20 mg L-1 ) showed HLB and XAD18-DGT devices were more stable compared to SXLA-DGT...
March 12, 2018: Water Research
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"