keyword
MENU ▼
Read by QxMD icon Read
search

Chemogenetics

keyword
https://www.readbyqxmd.com/read/29664924/evidence-supporting-a-role-for-astrocytes-in-the-regulation-of-cognitive-flexibility-and-neuronal-oscillations-through-the-ca2-binding-protein-s100%C3%AE
#1
Adam T Brockett, Gary A Kane, Patrick K Monari, Brandy A Briones, Pierre-Antoine Vigneron, Gabriela A Barber, Andres Bermudez, Uma Dieffenbach, Alexander D Kloth, Timothy J Buschman, Elizabeth Gould
The medial prefrontal cortex (mPFC) is important for cognitive flexibility, the ability to switch between two task-relevant dimensions. Changes in neuronal oscillations and alterations in the coupling across frequency ranges have been correlated with attention and cognitive flexibility. Here we show that astrocytes in the mPFC of adult male Sprague Dawley rats, participate in cognitive flexibility through the astrocyte-specific Ca2+ binding protein S100β, which improves cognitive flexibility and increases phase amplitude coupling between theta and gamma oscillations...
2018: PloS One
https://www.readbyqxmd.com/read/29662202/stimulation-of-entorhinal-cortex-dentate-gyrus-circuitry-is-antidepressive
#2
Sanghee Yun, Ryan P Reynolds, Iraklis Petrof, Alicia White, Phillip D Rivera, Amir Segev, Adam D Gibson, Maiko Suarez, Matthew J DeSalle, Naoki Ito, Shibani Mukherjee, Devon R Richardson, Catherine E Kang, Rebecca C Ahrens-Nicklas, Ivan Soler, Dane M Chetkovich, Saïd Kourrich, Douglas A Coulter, Amelia J Eisch
Major depressive disorder (MDD) is considered a 'circuitopathy', and brain stimulation therapies hold promise for ameliorating MDD symptoms, including hippocampal dysfunction. It is unknown whether stimulation of upstream hippocampal circuitry, such as the entorhinal cortex (Ent), is antidepressive, although Ent stimulation improves learning and memory in mice and humans. Here we show that molecular targeting (Ent-specific knockdown of a psychosocial stress-induced protein) and chemogenetic stimulation of Ent neurons induce antidepressive-like effects in mice...
April 16, 2018: Nature Medicine
https://www.readbyqxmd.com/read/29660441/silencing-the-insular-striatal-circuit-decreases-alcohol-self-administration-and-increases-sensitivity-to-alcohol
#3
Anel A Jaramillo, Kalynn Van Voorhies, Patrick A Randall, Joyce Besheer
Internal drug states/cues can impact drug taking, as pretreatment with a moderate to high alcohol dose (i.e., loading dose) can decrease subsequent alcohol self-administration, alcohol-seeking, and relapse-like drinking. The insular cortex (IC) is implicated in processing information about internal states and findings show that silencing the IC and its projections to the nucleus accumbens core (AcbC) enhance sensitivity to the interoceptive effects of alcohol. Therefore, the goal of the present work was to determine the functional role of IC-AcbC projections in modulating the effects of alcohol pretreatment on operant alcohol self-administration...
April 13, 2018: Behavioural Brain Research
https://www.readbyqxmd.com/read/29628879/dopamine-effort-based-choice-and-behavioral-economics-basic-and-translational-research
#4
REVIEW
John D Salamone, Merce Correa, Jen-Hau Yang, Renee Rotolo, Rose Presby
Operant behavior is not only regulated by factors related to the quality or quantity of reinforcement, but also by the work requirements inherent in performing instrumental actions. Moreover, organisms often make effort-related decisions involving economic choices such as cost/benefit analyses. Effort-based decision making is studied using behavioral procedures that offer choices between high-effort options leading to relatively preferred reinforcers vs. low effort/low reward choices. Several neural systems, including the mesolimbic dopamine (DA) system and other brain circuits, are involved in regulating effort-related aspects of motivation...
2018: Frontiers in Behavioral Neuroscience
https://www.readbyqxmd.com/read/29626564/dorsal-ca1-interneurons-contribute-to-acute-stress-induced-spatial-memory-deficits
#5
Jing-Ying Yu, Ping Fang, Chi Wang, Xing-Xing Wang, Kun Li, Qian Gong, Ben-Yan Luo, Xiao-Dong Wang
Exposure to severely stressful experiences disrupts the activity of neuronal circuits and impairs declarative memory. GABAergic interneurons coordinate neuronal network activity, but their involvement in stress-evoked memory loss remains to be elucidated. Here, we provide evidence that interneurons in area CA1 of the dorsal hippocampus partially modulate acute stress-induced memory deficits. In adult male mice, both acute forced swim stress and restraint stress impaired hippocampus-dependent spatial memory and increased the density of c-fos-positive interneurons in the dorsal CA1...
April 4, 2018: Neuropharmacology
https://www.readbyqxmd.com/read/29624516/neural-pathways-in-medial-septal-cholinergic-modulation-of-chronic-pain-distinct-contribution-of-anterior-cingulate-cortex-and-ventral-hippocampus
#6
Ying-Ying Jiang, Shan Shao, Yu Zhang, Jie Zheng, Xi Chen, Shuang Cui, Feng-Yu Liu, You Wan, Ming Yi
One specific behavior can be synergistically modulated by different neural pathways. Medial septal (MS) cholinergic system innervates widespread cortical and subcortical regions and participates in pain modulation, but the underlying neural pathways are not fully understood. The present study examined the contribution of MS cholinergic neurons and two neural pathways: MS-rostral anterior cingulate cortex (rACC) and MS-ventral hippocampal CA1 (vCA1), in modulating perceptual and affective pain behaviors in a mouse model of chronic inflammatory pain...
April 5, 2018: Pain
https://www.readbyqxmd.com/read/29623029/hypersensitivity-of-prelimbic-cortex-neurons-contributes-to-aggravated-nociceptive-responses-in-rats-with-experience-of-chronic-inflammatory-pain
#7
Xiao-Cen Fan, Su Fu, Feng-Yu Liu, Shuang Cui, Ming Yi, You Wan
Previous experience of chronic pain causes enhanced responses to upcoming noxious events in both humans and animals, but the underlying mechanisms remain unclear. In the present study, we found that rats with complete Freund's adjuvant (CFA)-induced chronic inflammatory pain experience exhibited aggravated pain responses to later formalin test. Enhanced neuronal activation upon formalin assaults and increased phosphorylated cAMP-response element binding protein (CREB) were observed in the prelimbic cortex (PL) of rats with chronic inflammatory pain experience, and inhibiting PL neuronal activities reversed the aggravated pain...
2018: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29618778/dynamic-interaction-of-local-and-transhemispheric-networks-is-necessary-for-progressive-intensification-of-hippocampal-seizures
#8
Fredrik Berglind, My Andersson, Merab Kokaia
The detailed mechanisms of progressive intensification of seizures often occurring in epilepsy are not well understood. Animal models of kindling, with progressive intensification of stimulation-induced seizures, have been previously used to investigate alterations in neuronal networks, but has been obscured by limited recording capabilities during electrical stimulations. Remote networks in kindling have been studied by physical deletions of the connected structures or pathways, inevitably leading to structural reorganisations and related adverse effects...
April 4, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29618134/melanin-concentrating-hormone-expressing-neurons-adjust-slow-wave-sleep-dynamics-to-catalyze-paradoxical-rem-sleep
#9
Christophe Varin, Pierre-Hervé Luppi, Patrice Fort
Study Objectives: Experimental studies over the last fifteen years established a role in sleep of the tuberal hypothalamic neurons that express melanin-concentrating hormone (MCH). Controversies still remain regarding their actual contribution to both slow-wave sleep (SWS) and paradoxical sleep (PS aka REM sleep) or PS alone. Methods: To address this point, we compared effects of chemogenetic activation and inhibition of MCH neurons on SWS and PS amounts and EEG rhythmic activities in transgenic Pmch-cre mice...
March 29, 2018: Sleep
https://www.readbyqxmd.com/read/29556030/circuit-dissection-of-the-role-of-somatostatin-in-itch-and-pain
#10
Jing Huang, Erika Polgár, Hans Jürgen Solinski, Santosh K Mishra, Pang-Yen Tseng, Noboru Iwagaki, Kieran A Boyle, Allen C Dickie, Mette C Kriegbaum, Hendrik Wildner, Hanns Ulrich Zeilhofer, Masahiko Watanabe, John S Riddell, Andrew J Todd, Mark A Hoon
Stimuli that elicit itch are detected by sensory neurons that innervate the skin. This information is processed by the spinal cord; however, the way in which this occurs is still poorly understood. Here we investigated the neuronal pathways for itch neurotransmission, particularly the contribution of the neuropeptide somatostatin. We find that in the periphery, somatostatin is exclusively expressed in Nppb+ neurons, and we demonstrate that Nppb+ somatostatin+ cells function as pruriceptors. Employing chemogenetics, pharmacology and cell-specific ablation methods, we demonstrate that somatostatin potentiates itch by inhibiting inhibitory dynorphin neurons, which results in disinhibition of GRPR+ neurons...
March 19, 2018: Nature Neuroscience
https://www.readbyqxmd.com/read/29551417/oxytocin-signaling-in-the-lateral-septum-prevents-social-fear-during-lactation
#11
Rohit Menon, Thomas Grund, Iulia Zoicas, Ferdinand Althammer, Dominik Fiedler, Verena Biermeier, Oliver J Bosch, Yuichi Hiraoka, Katsuhiko Nishimori, Marina Eliava, Valery Grinevich, Inga D Neumann
Oxytocin (OXT)-mediated behavioral responses to social and stressful cues have extensively been studied in male rodents. Here, we investigated the capacity of brain OXT receptor (OXTR) signaling in the lateral septum (LS) to prevent social fear expression in female mice using the social-fear-conditioning paradigm. Utilizing the activated OXT system during lactation, we show that lactating mice did not express fear 24 hr after social fear conditioning. Supporting the role of OXTR signaling in the LS in attenuation of social fear, synthetic OXT infusion or overexpression of OXTR in the LS diminished social fear expression, whereas constitutive OXTR knockout severely impaired social fear extinction in virgin mice...
March 7, 2018: Current Biology: CB
https://www.readbyqxmd.com/read/29550367/contemporary-strategies-for-dissecting-the-neuronal-basis-of-neurodevelopmental-disorders
#12
Dong-Oh Seo, Laura E Motard, Michael R Bruchas
Great efforts in clinical and basic research have shown progress in understanding the neurobiological mechanisms of neurodevelopmental disorders, such as autism, schizophrenia, and attention-deficit hyperactive disorders. Literature on this field have suggested that these disorders are affected by the complex interaction of genetic, biological, psychosocial and environmental risk factors. However, this complexity of interplaying risk factors during neurodevelopment has prevented a complete understanding of the causes of those neuropsychiatric symptoms...
March 14, 2018: Neurobiology of Learning and Memory
https://www.readbyqxmd.com/read/29536948/optogenetic-dissection-of-ictogenesis-in-search-of-a-targeted-anti-epileptic-therapy
#13
Kyle P Lillis, Kevin J Staley
For over a century, epileptic seizures have been characterized as a state of pathological, hypersynchronous brain activity. Anti-epileptic therapies have been developed largely based on the dogma that the altered brain rhythms result from an overabundance of glutamatergic activity or insufficient GABAergic inhibition. The most effective drugs in use today act to globally decrease excitation, increase inhibition, or decrease all activity. Unfortunately, such broad alterations to brain activity often lead to impactful side effects such as mood disordersdrowsiness, cognitive impairment, and sleep disruption...
March 14, 2018: Journal of Neural Engineering
https://www.readbyqxmd.com/read/29533926/reducing-cxcr4-mediated-nociceptor-hyperexcitability-reverses-painful-diabetic-neuropathy
#14
Nirupa D Jayaraj, Bula J Bhattacharyya, Abdelhak A Belmadani, Dongjun Ren, Craig A Rathwell, Sandra Hackelberg, Brittany E Hopkins, Herschel R Gupta, Richard J Miller, Daniela M Menichella
Painful diabetic neuropathy (PDN) is an intractable complication of diabetes that affects 25% of patients. PDN is characterized by neuropathic pain and small-fiber degeneration, accompanied by dorsal root ganglion (DRG) nociceptor hyperexcitability and loss of their axons within the skin. The molecular mechanisms underlying DRG nociceptor hyperexcitability and small-fiber degeneration in PDN are unknown. We hypothesize that chemokine CXCL12/CXCR4 signaling is central to this mechanism, as we have shown that CXCL12/CXCR4 signaling is necessary for the development of mechanical allodynia, a pain hypersensitivity behavior common in PDN...
March 13, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29507308/circuit-specific-and-neuronal-subcellular-wide-e-i-balance-in-cortical-pyramidal-cells
#15
Weiguo Yang, Qian-Quan Sun
We used ChR2-assisted circuit mapping (CRACM) to examine neuronal/compartmental excitatory and inhibitory synaptic balance (E-I balance) in pyramidal cells (PCs) located in several brain regions (including both neocortices and paleocortices). Within the vS1, different inputs on the same neurons, or the same inputs formed on different targets, induced different E/I ratios. E/I ratios in PCs from different regions were largely different. Chemogenetic silencing of somatostatin (SOM)- or parvalbumin (PV)-containing interneurons (INs) while optogenetically activating long-range M1 inputs demonstrated differential contribution of PV and SOM INs to the E/I ratios in a layer-specific manner in S1...
March 5, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29507292/fear-extinction-requires-infralimbic-cortex-projections-to-the-basolateral-amygdala
#16
Daniel W Bloodgood, Jonathan A Sugam, Andrew Holmes, Thomas L Kash
Fear extinction involves the formation of a new memory trace that attenuates fear responses to a conditioned aversive memory, and extinction impairments are implicated in trauma- and stress-related disorders. Previous studies in rodents have found that the infralimbic prefrontal cortex (IL) and its glutamatergic projections to the basolateral amygdala (BLA) and basomedial amygdala (BMA) instruct the formation of fear extinction memories. However, it is unclear whether these pathways are exclusively involved in extinction, or whether other major targets of the IL, such as the nucleus accumbens (NAc) also play a role...
March 6, 2018: Translational Psychiatry
https://www.readbyqxmd.com/read/29507147/activity-dependent-myelination-of-parvalbumin-interneurons-mediated-by-axonal-morphological-plasticity
#17
J Stedehouder, D Brizee, G Shpak, S A Kushner
Axonal myelination of neocortical pyramidal neurons is dynamically modulated by neuronal activity. Recent studies have shown that a substantial proportion of neocortical myelin content is contributed by fast-spiking, parvalbumin (PV)-positive interneurons. However, it remains unknown whether the myelination of PV+ interneurons is also modulated by intrinsic activity. Here, we utilized cell-type specific Designer Receptors Exclusively Activated by Designer Drugs (DREADDs) in adult male and female mice to activate a sparse population of medial prefrontal cortex PV+ interneurons...
March 5, 2018: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/29502952/stress-accelerates-defensive-responses-to-looming-in-mice-and-involves-a-locus-coeruleus-superior-colliculus-projection
#18
Lei Li, Xiaolong Feng, Zheng Zhou, Huiqi Zhang, Qianqian Shi, Zhuogui Lei, Peilei Shen, Qingning Yang, Binghao Zhao, Shuran Chen, Lin Li, Yulin Zhang, Pengjie Wen, Zhonghua Lu, Xiang Li, Fuqiang Xu, Liping Wang
Defensive responses to threatening stimuli are crucial to the survival of species. While expression of these responses is considered to be instinctive and unconditional, their magnitude may be affected by environmental and internal factors. The neural circuits underlying this modulation are still largely unknown. In mice, looming-evoked defensive responses are mediated by the superior colliculus (SC), a subcortical sensorimotor integration center. We found that repeated stress caused an anxiety-like state in mice and accelerated defensive responses to looming...
February 26, 2018: Current Biology: CB
https://www.readbyqxmd.com/read/29500819/camp-producing-chemogenetic-activation-of-indirect-pathway-striatal-projection-neurons-and-the-downstream-effects-on-the-globus-pallidus-and-subthalamic-nucleus-in-freely-moving-mice
#19
Safa Bouabid, Fu-Ming Zhou
The indirect pathway striatal medium spiny projection neurons (iMSNs) are critical to motor and cognitive brain functions. These neurons express a high level of cAMP-increasing adenosine A2a receptors (A2aRs). However, the potential effects of cAMP production on iMSN spiking activity have not been established, and recording identified iMSNs in freely moving animals is challenging. Here we show that in the transgenic mice expressing cAMP-producing G protein Gs -coupled designer receptor exclusively activated by designer drug (Gs-DREADD) in iMSNs, the baseline spike firing in MSNs is normal, indicating DREADD expression does not affect the normal physiology of these neurons...
March 3, 2018: Journal of Neurochemistry
https://www.readbyqxmd.com/read/29490273/action-selection-and-flexible-switching-controlled-by-the-intralaminar-thalamic-neurons
#20
Shigeki Kato, Ryoji Fukabori, Kayo Nishizawa, Kana Okada, Nozomu Yoshioka, Masateru Sugawara, Yuko Maejima, Kenju Shimomura, Masahiro Okamoto, Satoshi Eifuku, Kazuto Kobayashi
Learning processes contributing to appropriate selection and flexible switching of behaviors are mediated through the dorsal striatum, a key structure of the basal ganglia circuit. The major inputs to striatal subdivisions are provided from the intralaminar thalamic nuclei, including the central lateral nucleus (CL) and parafascicular nucleus (PF). Thalamostriatal neurons in the PF modulate the acquisition and performance of stimulus-response learning. Here, we address the roles of the CL thalamostriatal neurons in learning processes by using a selective neural pathway targeting technique...
February 27, 2018: Cell Reports
keyword
keyword
49247
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"