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https://www.readbyqxmd.com/read/28331408/on-some-new-species-of-ancorabolidae-sars-1909-from-the-gulf-of-california-the-genera-ceratonotus-sars-1909-and-dendropsyllus-conroy-dalton-2003-crustacea-copepoda-harpacticoida
#1
Samuel Gómez, Karen Díaz
Two new species of two genera of the family Ancorabolidae, Ceratonotus elongatussp. n. and Dendropsyllus californiensissp. n., found at 1642 m and 1759 m depth, respectively, in the Southern Trough of Guaymas Basin, are described. Ceratonotus elongatussp. n. was attributed to that genus by a series of character states of which the lack of dendroid dorsal processes on the P6-bearing somite and the presence of such processes on the first abdominal somite were definitive. This species was observed to be very close to Ceratonotus thistlei Conroy-Dalton, 2003 from the San Diego Trough, and can be separated by a number of traits of which the elongated sensilla-bearing dorsal tubercles on the second abdominal somite in the new species was definitive...
2017: ZooKeys
https://www.readbyqxmd.com/read/28287551/temporal-ordering-of-dynamic-expression-data-from-detailed-spatial-expression-maps
#2
Charlotte S L Bailey, Robert A Bone, Philip J Murray, J Kim Dale
During somitogenesis, pairs of epithelial somites form in a progressive manner, budding off from the anterior end of the pre-somitic mesoderm (PSM) with a strict species-specific periodicity. The periodicity of the process is regulated by a molecular oscillator, known as the "segmentation clock," acting in the PSM cells. This clock drives the oscillatory patterns of gene expression across the PSM in a posterior-anterior direction. These so-called clock genes are key components of three signaling pathways: Wnt, Notch, and fibroblast growth factor (FGF)...
February 9, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28253363/mutation-in-cpsf6-cfim68-cleavage-and-polyadenylation-specificity-factor-subunit-6-causes-short-3-utrs-and-disturbs-gene-expression-in-developing-embryos-as-revealed-by-an-analysis-of-primordial-germ-cell-migration-using-the-medaka-mutant-naruto
#3
Takao Sasado, Hisato Kondoh, Makoto Furutani-Seiki, Kiyoshi Naruse
Our previous studies analyzing medaka mutants defective in primordial germ cell (PGC) migration identified cxcr4b and cxcr7, which are both receptors of the chemokine sdf1/cxcl12, as key regulators of PGC migration. Among PGC migration mutants, naruto (nar) is unique in that the mutant phenotype includes gross morphological abnormalities of embryos, suggesting that the mutation affects a broader range of processes. A fine genetic linkage mapping and genome sequencing showed the nar gene encodes Cleavage and Polyadenylation Specificity Factor subunit 6 (CPSF6/CFIm68)...
2017: PloS One
https://www.readbyqxmd.com/read/28240769/developmental-mechanisms-of-macroevolutionary-change-in-the-tetrapod-axis-a-case-study-of-sauropterygia
#4
Laura C Soul, Roger B J Benson
Understanding how developmental processes change on macroevolutionary timescales to generate body plan disparity is fundamental to the study of vertebrate evolution. Adult morphology of the vertebral column directly reflects the mechanisms that generate vertebral counts (somitogenesis) and their regionalisation (homeotic effects) during embryonic development. Sauropterygians were a group of Mesozoic marine reptiles that exhibited an extremely high disparity of presacral vertebral/somite counts. Using phylogenetic comparative methods, we demonstrate that somitogenesis and homeotic effects evolved in a co-ordinated way among sauropterygians, contrasting with the wider pattern in tetrapods, in which somitogenetic and homeotic shifts are uncorrelated...
February 27, 2017: Evolution; International Journal of Organic Evolution
https://www.readbyqxmd.com/read/28237626/effects-of-cryopreservation-at-various-temperatures-on-the-survival-of-kelp-grouper-epinephelus-moara-embryos-from-fertilization-with-cryopreserved-sperm
#5
Yongsheng Tian, Zhangfan Chen, Jiang Tang, Huimin Duan, Jieming Zhai, Bo Li, Wenhui Ma, Jiangchun Liu, Yunxia Hou, Zhengxiang Sun
Fish embryo cryopreservation is highly important for the long-term preservation of genomic and genetic information; however, few successful cases of fish embryo cryopreservation have been reported over the past 60 years. This is the first study to use Epinephelus moara embryos from fertilization with cryopreserved sperm as experimental material. Embryos that developed to the 16-22 somite stage and tail-bud stage were treated with the vitrification solution PMG3T according to a five-step equilibration method and cryopreserved at various temperatures and storage duration...
February 22, 2017: Cryobiology
https://www.readbyqxmd.com/read/28214321/pr%C3%A3-und-perioperative-aspekte-der-versorgung-dermatochirurgischer-patienten
#6
Cornelia S L Müller, Wakiko Hubner, Sigrid Thieme-Ruffing, Claudia Pföhler, Thomas Vogt, Thomas Volk, Barbara C Gärtner, Patric Bialas
Die Dermatochirurgie nimmt hinsichtlich vieler Punkte eine Sonderstellung unter den operativen Fächern ein. Hierzu gehört in erster Linie die Tatsache, dass bis auf wenige Ausnahmen fast alle Eingriffe traditionell in Lokal- bzw. Regionalanästhesie und oft auch in räumlich-infrastruktureller Trennung von den großen Zentral-Operationssälen stattfinden können. Die peri- und postoperative Überwachung obliegt dabei dem dermatochirurgischen Operationsteam. Das sui generis kleinere OP-Team hat somit eine ganze Reihe perioperativer Notwendigkeiten zu beachten, um die sich in den "großen" chirurgischen Fächern eine Vielzahl verschiedener beteiligter Fachgruppen gemeinsam kümmern...
February 2017: Journal der Deutschen Dermatologischen Gesellschaft, Journal of the German Society of Dermatology: JDDG
https://www.readbyqxmd.com/read/28187574/first-zoeal-stage-of-the-hermit-crab-i-enallopaguropsis-i-i-guatemoci-i-glassell-1937-crustacea-anomura-paguroidea-paguridea-obtained-in-the-laboratory
#7
Manuel Ayón-Parente, Michel E Hendrickx
The first larval stage of Enallopaguropsis guatemoci is described in detail and illustrated. The larva is compared with larvae known for other genera of Paguridae except Pagurus. A synthesis of all characters provided herein indicates that first larvae of E. guatemoci can be separated from all known pagurid larvae (except Pagurus) by the combination of the following characters: carapace with long dorsal carina, posterolateral spines absent; abdominal somites 3-5 with pair of moderately long spines; telson narrowly triangular, not forked; spine at antennal protopod as long as endopod...
February 3, 2017: Zootaxa
https://www.readbyqxmd.com/read/28183698/constitutively-active-notch1-converts-cranial-neural-crest-derived-frontonasal-mesenchyme-to-perivascular-cells-in-vivo
#8
Sophie R Miller, Surangi N Perera, Clare V H Baker
Perivascular/mural cells originate from either the mesoderm or the cranial neural crest. Regardless of their origin, Notch signalling is necessary for their formation. Furthermore, in both chicken and mouse, constitutive Notch1 activation (via expression of the Notch1 intracellular domain) is sufficient in vivo to convert trunk mesoderm-derived somite cells to perivascular cells, at the expense of skeletal muscle. In experiments originally designed to investigate the effect of premature Notch1 activation on the development of neural crest-derived olfactory ensheathing glial cells (OECs), we used in ovo electroporation to insert a tetracycline-inducible NotchΔE construct (encoding a constitutively active mutant of mouse Notch1) into the genome of chicken cranial neural crest cell precursors, and activated NotchΔE expression by doxycycline injection at embryonic day 4...
March 15, 2017: Biology Open
https://www.readbyqxmd.com/read/28178531/in%C3%A2-vivo-human-somitogenesis-guides-somite-development-from-hpscs
#9
Haibin Xi, Wakana Fujiwara, Karen Gonzalez, Majib Jan, Simone Liebscher, Ben Van Handel, Katja Schenke-Layland, April D Pyle
Somites form during embryonic development and give rise to unique cell and tissue types, such as skeletal muscles and bones and cartilage of the vertebrae. Using somitogenesis-stage human embryos, we performed transcriptomic profiling of human presomitic mesoderm as well as nascent and developed somites. In addition to conserved pathways such as WNT-β-catenin, we also identified BMP and transforming growth factor β (TGF-β) signaling as major regulators unique to human somitogenesis. This information enabled us to develop an efficient protocol to derive somite cells in vitro from human pluripotent stem cells (hPSCs)...
February 7, 2017: Cell Reports
https://www.readbyqxmd.com/read/28166301/stimulus-triggered-enhancement-of-chilling-tolerance-in-zebrafish-embryos
#10
Bernadett Faragó, Tímea Kollár, Katalin Szabó, Csilla Budai, Eszter Losonczi, Gergely Bernáth, Zsolt Csenki-Bakos, Béla Urbányi, Csaba Pribenszky, Ákos Horváth, Judit Cserepes
BACKGROUND: Cryopreservation of zebrafish embryos is still an unsolved problem despite market demand and massive efforts to preserve genetic variation among numerous existing lines. Chilled storage of embryos might be a step towards developing successful cryopreservation, but no methods to date have worked. METHODS: In the present study, we applied a novel strategy to improve the chilling tolerance of zebrafish embryos by introducing a preconditioning hydrostatic pressure treatment to the embryos...
2017: PloS One
https://www.readbyqxmd.com/read/28160066/pax2-pax8-defined-subdomains-and-the-occurrence-of-apoptosis-in-the-posterior-placodal-area-of-mice
#11
Stefan Washausen, Wolfgang Knabe
The present work aims to improve our understanding of the causes and functions of apoptosis during the morphogenesis of epibranchial placodes in mice. Schematic maps helped to compare the spatiotemporal sequence of apoptotic events with the protein expression patterns of general (Six1) and specific placodal markers (Pax2, Pax8). Our findings challenge the view that, in mammals, all three epibranchial placodes spring from the original posterior placodal area (PPA) of presomite or early somite embryos. Instead, close-meshed analysis of the Pax2/Pax8 expression patterns demonstrates the stepwise emergence of two subdomains which both belong to the gradually expanding PPA, and which largely give rise to the otic placode and epibranchial placode 1 (anterior subdomain), or to the caudal epibranchial placodes (posterior subdomain)...
February 3, 2017: Brain Structure & Function
https://www.readbyqxmd.com/read/28130672/a-new-species-of-hamaticolax-ho-lin-2006-copepoda-bomolochidae-from-deep-water-macrourids-in-the-mediterranean
#12
David Pérez-I-García, Maite Carrassón, Geoffrey Allan Boxshall
Hamaticolax resupinus n. sp. is described from specimens collected from the gill cavities of Coelorinchus mediterraneus Iwamoto & Ungaro and Coryphaenoides mediterraneus (Giglioli) (Gadiformes: Macrouridae) caught in the Western Mediterranean Sea at depths between 1,236 and 1,626 m. Hamaticolax resupinus n. sp. closely resembles H. maleus Oldewage, 1994, but differs from the latter by its smaller body size and in having a genital double-somite in the female that is markedly wider than the free abdominal somites and has strongly convex lateral margins...
February 2017: Systematic Parasitology
https://www.readbyqxmd.com/read/28122043/correction-somite-derived-retinoic-acid-regulates-zebrafish-hematopoietic-stem-cell-formation
#13
Laura M Pillay, Kacey J Mackowetzky, Sonya A Widen, Andrew Jan Waskiewicz
[This corrects the article DOI: 10.1371/journal.pone.0166040.].
2017: PloS One
https://www.readbyqxmd.com/read/28101674/posterior-tail-development-in-the-salamander-eurycea-cirrigera-exploring-cellular-dynamics-across-life-stages
#14
Janet L Vaglia, Chet Fornari, Paula K Evans
During embryogenesis, the body axis elongates and specializes. In vertebrate groups such as salamanders and lizards, elongation of the posterior body axis (tail) continues throughout life. This phenomenon of post-embryonic tail elongation via addition of vertebrae has remained largely unexplored, and little is known about the underlying developmental mechanisms that promote vertebral addition. Our research investigated tail elongation across life stages in a non-model salamander species, Eurycea cirrigera (Plethodontidae)...
January 18, 2017: Development Genes and Evolution
https://www.readbyqxmd.com/read/28100224/radical-scavenging-activities-of-lagerstroemia-speciosa-l-pers-petal-extracts-and-its-hepato-protection-in-ccl4-intoxicated-mice
#15
Bipransh Kumar Tiwary, Somit Dutta, Priyankar Dey, Mossaraf Hossain, Anoop Kumar, Sony Bihani, Ashis Kumar Nanda, Tapas Kumar Chaudhuri, Ranadhir Chakraborty
BACKGROUND: Lagerstroemia speciosa (L.) Pers. has medicinal importance. Bioactive phytochemicals isolated from different parts of L. speciosa, have revealed hypoglycemic, antibacterial, anti-inflammatory, antioxidant and hepato protective properties. Despite one report from Philippines detailing the use of L. speciosa as curative for fever and as well as diuretic, there is no experimental evidence about the hepatoprotective activity of the flower extracts. METHODS: Several spectroscopic methods, including GC-MS, were used to characterize phytochemicals present in the petal extract of L...
January 18, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28097653/prolongation-of-somitogenesis-in-two-anguilliform-species-the-japanese-eel-anguilla-japonica-and-pike-eel-muraenesox-cinereus-with-refined-descriptions-of-their-early-development
#16
T Kawakami, Y Yamada, S Tanaka, K Tsukamoto
The embryonic development of the Japanese eel Anguilla japonica and pike eel Muraenesox cinereus was morphologically investigated with laboratory-reared specimens to clarify the characteristics of somitogenesis. In A. japonica, somites were first observed at 18 h post fertilization (hpf) when epiboly reached 90%. Somitogenesis progressed at a rate of 1·6 h(-1) at mean ± s.d. 22·6 ± 0·7° C and completed at 107 hpf (3 days post hatching; dph) when total number of somites (ST) reached 114, which corresponds to the species' number of vertebrae (112-119)...
January 18, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28096216/basal-filopodia-and-vascular-mechanical-stress-organize-fibronectin-into-pillars-bridging-the-mesoderm-endoderm-gap
#17
Yuki Sato, Kei Nagatoshi, Ayumi Hamano, Yuko Imamura, David Huss, Seiichi Uchida, Rusty Lansford
Cells may exchange information with other cells and tissues by exerting forces on the extracellular matrix (ECM). Fibronectin (FN) is an important ECM component that forms fibrils through cell contacts and creates directionally biased geometry. Here, we demonstrate that FN is deposited as pillars between widely separated germ layers, namely the somitic mesoderm and the endoderm, in quail embryos. Alongside the FN pillars, long filopodia protrude from the basal surfaces of somite epithelial cells. Loss-of-function of Ena/VASP, α5β1-integrins or talin in the somitic cells abolished the FN pillars, indicating that FN pillar formation is dependent on the basal filopodia through these molecules...
January 15, 2017: Development
https://www.readbyqxmd.com/read/28087631/papc-couples-the-segmentation-clock-to-somite-morphogenesis-by-regulating-n-cadherin-dependent-adhesion
#18
Jérome Chal, Charlène Guillot, Olivier Pourquié
Vertebrate segmentation is characterized by the periodic formation of epithelial somites from the mesenchymal presomitic mesoderm (PSM). How the rhythmic signaling pulse delivered by the segmentation clock is translated into the periodic morphogenesis of somites remains poorly understood. Here, we focused on the role of paraxial protocadherin (PAPC/Pcdh8) in this process. We showed that in chicken and mouse embryos, PAPC expression is tightly regulated by the clock and wavefront system in the posterior PSM...
February 15, 2017: Development
https://www.readbyqxmd.com/read/28060338/a-submerged-filter-paper-sandwich-for-long-term-ex-ovo-time-lapse-imaging-of-early-chick-embryos
#19
Manuel Schmitz, Ben K A Nelemans, Theodoor H Smit
Due to its availability, low cost, flat geometry, and transparency, the ex ovo chick embryo has become a major vertebrate animal model for the study of morphogenetic events, such as gastrulation(2), neurulation(3)(-)(5), somitogenesis(6), heart bending(7,8), and brain formation(9)(-)(13), during early embryogenesis. Key to understanding morphogenetic processes is to follow them dynamically by time-lapse imaging. The acquisition of time-lapse movies of chick embryogenesis ex ovo has been limited either to short time windows or to the need for an incubator to control temperature and humidity around the embryo(14)...
December 28, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28049690/rewiring-of-embryonic-glucose-metabolism-via-suppression-of-pfk-1-and-aldolase-during-mouse-chorioallantoic-branching
#20
Hidenobu Miyazawa, Yoshifumi Yamaguchi, Yuki Sugiura, Kurara Honda, Koki Kondo, Fumio Matsuda, Takehiro Yamamoto, Makoto Suematsu, Masayuki Miura
Adapting the energy metabolism state to changing bioenergetic demands is essential for mammalian development accompanying massive cell proliferation and cell differentiation. However, it remains unclear how developing embryos meet the changing bioenergetic demands during the chorioallantoic branching (CB) stage, when the maternal-fetal exchange of gases and nutrients is promoted. In this study, using metabolome analysis with mass-labeled glucose, we found that developing embryos redirected glucose carbon flow into the pentose phosphate pathway via suppression of the key glycolytic enzymes PFK-1 and aldolase during CB...
January 1, 2017: Development
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