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https://www.readbyqxmd.com/read/29785502/comparative-persistence-of-human-sewage-specific-enterococcal-bacteriophages-in-freshwater-and-seawater
#1
Namfon Booncharoen, Skorn Mongkolsuk, Kwanrawee Sirikanchana
Enterococcus faecalis bacteria have been recently reported for their ability to host bacteriophages that are specifically from human sewage, suggesting their application to track human fecal contamination in water resources. However, little is known about the survivability of sewage-specific enterococcal bacteriophages in various water matrices under ambient and storage conditions. In this study, bacteriophages that were derived from the Thailand-isolated E. faecalis strains AIM06 and SR14 exhibited morphologies consistent with the Siphoviridae, Podoviridae, and Myoviridae families...
May 21, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29785473/changes-in-melatonin-cortisol-and-body-temperature-and-the-relationship-between-endogenous-melatonin-levels-and-analgesia-consumption-in-patients-undergoing-bariatric-surgery
#2
Neslihan Altunkaya, Mehmet Ali Erdogan, Ulku Ozgul, Mukadder Sanli, Muharrem Ucar, Onural Ozhan, Fatih Sumer, Selim Erdogan, Cemil Colak, Mahmut Durmus
BACKGROUND: Melatonin has analgesic, anti-inflammatory, sedative, and anxiolytic properties. However, the relationship between endogenous melatonin levels and postoperative analgesic requirements has not been well elucidated in patients undergoing bariatric surgery. We studied endogenous melatonin levels, cortisol levels, body temperatures, and the relationship between the level of endogenous melatonin and postoperative morphine consumption. METHODS: The trial was conducted among 30 patients who were scheduled for laparoscopic bariatric surgery...
May 21, 2018: Obesity Surgery
https://www.readbyqxmd.com/read/29784321/iridoid-blue-based-pigments-of-genipa-americana-l-rubiaceae-extract-influence-of-ph-and-temperature-on-color-stability-and-antioxidant-capacity-during-in-vitro-simulated-digestion
#3
Iramaia Angélica Neri-Numa, Celio Fernando Figueiredo Angolini, Juliano Lemos Bicas, Ana Lucia Tasca Gois Ruiz, Glaucia Maria Pastore
Iridoid blue-based pigments (IBBP) extract of Genipa americana L. represents a natural alternative additive for food applications and also exerts desirable biological effects on human health. In this study the iridoids present in the extract were identified, the influence of pH and temperature on color difference (ΔE) of IBBP was evaluated using a central composite design (CCD) and finally the antioxidant capacity was monitored before and after its in vitro digestion. Ten glucoside iridoids were detected and the main compounds identified were genipin, genipin 1-β-gentiobioside and geniposide...
October 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29784295/use-of-fourier-transform-infrared-spectroscopy-to-monitor-sugars-in-the-beer-mashing-process
#4
Francylaine Silva de Almeida, Cinthia Aparecida de Andrade Silva, Sandro Marcio Lima, Yzel Rondon Suarez, Luis Humberto da Cunha Andrade
Mashing is an enzymatic procedure for the extraction of sugars from malt. It has strong temperature dependence, so monitoring the sugar production is very important for optimization of the brewing process. In this work, Fourier transform infrared spectroscopy (FTIR) was used to monitor three different mashing programs. These all presented high concentrations of maltose and dextrin, enabling their differentiation according to the FTIR absorption bands at 991 and 1022 cm-1 , characteristic of maltose and dextrin, respectively...
October 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29784077/effects-of-ketamine-compared-with-urethane-anesthesia-on-vestibular-sensory-evoked-potentials-and-systemic-physiology-in-mice
#5
Choongheon Lee, Timothy A Jones
The injectable anesthetic mixture ketamine-xylazine is commonly used for electrophysiologic experiments in laboratory animals, especially rodents. General anesthesia can induce significant changes in systemic physiology, including those that compromise neural function, thus introducing research confounds. The extent of such concerns varies by agent. Here in mice, we compared the effects of ketamine-xylazine and urethane-xylazine anesthesia on systemic physiologic parameters and the vestibular sensory evoked potential (VsEP), a tool used commonly to assess peripheral vestibular function...
May 1, 2018: Journal of the American Association for Laboratory Animal Science: JAALAS
https://www.readbyqxmd.com/read/29783742/study-of-cmos-soi-integrated-temperature-sensing-circuits-for-on-chip-temperature-monitoring
#6
Maria Malits, Igor Brouk, Yael Nemirovsky
This paper investigates the concepts, performance and limitations of temperature sensing circuits realized in complementary metal-oxide-semiconductor (CMOS) silicon on insulator (SOI) technology. It is shown that the MOSFET threshold voltage ( Vt ) can be used to accurately measure the chip local temperature by using a Vt extractor circuit. Furthermore, the circuit's performance is compared to standard circuits used to generate an accurate output current or voltage proportional to the absolute temperature, i...
May 19, 2018: Sensors
https://www.readbyqxmd.com/read/29783706/effect-of-temperature-changes-on-serum-protein-adsorption-on-thermoresponsive-cell-culture-surfaces-monitored-by-a-quartz-crystal-microbalance-with-dissipation
#7
Jun Kobayashi, Yoshinori Arisaka, Nobuhiko Yui, Yoshikatsu Akiyama, Masayuki Yamato, Teruo Okano
Thermoresponsive cell-culture polystyrene (PS) surfaces that are grafted with poly( N -isopropylacrylamide) (PIPAAm) facilitate the cultivation of cells at 37 °C and the detachment of cultured cells as a sheet with an underlying extracellular matrix (ECM) by reducing the temperature. However, the ECM and cell detachment mechanisms are still unclear because the detachment of cells from thermoresponsive surfaces is governed by complex interactions among the cells/ECM/surface. To explore the dynamic behavior of serum protein adsorption/desorption, thermoresponsive surfaces that correspond to thermoresponsive tissue-culture PS dishes were formed on sensor chips for quartz crystal microbalance with dissipation (QCM-D) measurements...
May 18, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29783131/effect-of-trace-element-addition-and-increasing-organic-loading-rates-on-the-anaerobic-digestion-of-cattle-slaughterhouse-wastewater
#8
Thomas Schmidt, Bernadette K McCabe, Peter W Harris, Seonmi Lee
In this study, anaerobic digestion of slaughterhouse wastewater with the addition of trace elements was monitored for biogas quantity, quality and process stability using CSTR digesters operated at mesophilic temperature. The determination of trace element concentrations was shown to be deficient in Fe, Ni, Co, Mn and Mo compared to recommendations given in the literature. Addition of these trace elements resulted in enhanced degradation efficiency, higher biogas production and improved process stability. Higher organic loading rates and lower hydraulic retention times were achieved in comparison to the control digesters...
May 18, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29782664/stereoselective-hplc-separation-of-alvimopan-on-cellulose-based-immobilized-polysaccharide-as-a-chiral-stationary-phase
#9
Nitin H Dhekale, Dattatray B Gunjal, Anil H Gore, Yagnakirankumar Komaravolu, K Hima Bindu, Govind B Kolekar
Chiral separation by normal phase high performance liquid chromatography is one of the most powerful technique to quantify the chiral purity of the compounds. In this study, a novel, simple, and specific analytical method was proposed to ascertain the chiral purity of alvimopan (ALV). The normal phase HPLC method was developed based on cellulose tris (3,5-dichlorophenylcarbamate) stationary phase. The separation of ALV isomers achieved by using column CHIRALPAK IC (250 × 4.6 mm, 5 μm), mobile phase n-hexane: isopropyl alcohol: ethanol: diethylamine (650:200:150:5 v/v), column oven temperature 30°C, flow rate 1...
May 21, 2018: Chirality
https://www.readbyqxmd.com/read/29780677/the-2016-southeastern-us-drought-an-extreme-departure-from-centennial-wetting-and-cooling
#10
A Park Williams, Benjamin I Cook, Jason E Smerdon, Daniel A Bishop, Richard Seager, Justin S Mankin
The fall 2016 drought in the southeastern United States (SE US) appeared exceptional based on its widespread impacts, but the current monitoring framework that only extends from 1979-present does not readily facilitate evaluation of soil-moisture anomalies in a centennial context. A new method to extend monthly gridded soil-moisture estimates back to 1895 is developed, indicating that since 1895, October-November 2016 soil moisture (0-200 cm) in the SE US was likely the second lowest on record, behind 1954...
October 27, 2017: Journal of Geophysical Research. Atmospheres: JGR
https://www.readbyqxmd.com/read/29780521/translating-molecular-detections-into-a-simple-temperature-test-using-a-target-responsive-smart-thermometer
#11
Jingjing Zhang, Hang Xing, Yi Lu
While it has been well recognized that affordable and pocket-size devices play a major role in environmental monitoring, food safety and medical diagnostics, it often takes a tremendous amount of resources to develop such devices. Devices that have been developed are often dedicated devices that can detect only one or a few targets. To overcome these limitations, we herein report a novel target-responsive smart thermometer for translating molecular detection into a temperature test. The sensor system consists of a functional DNA-phospholipase A2 (PLA2 ) enzyme conjugate, a liposome-encapsulated NIR dye, and a thermometer interfaced with a NIR-laser device...
April 28, 2018: Chemical Science
https://www.readbyqxmd.com/read/29780215/expression-of-a-rice-soluble-starch-synthase-gene-in-transgenic-wheat-improves-the-grain-yield-under-heat-stress-conditions
#12
Bin Tian, Shyamal K Talukder, Jianming Fu, Allan K Fritz, Harold N Trick
Wheat ( Triticum aestivum L.) is a temperate cereal with an optimum temperature range of 15-22°C during the grain filling stage. Heat stress is one of the major environmental constraints for wheat production worldwide. Temperatures above 25°C during the grain filling stage significantly reduced wheat yield and quality. This reduction was reported due to the inactivation of the soluble starch synthase, a key heat-labile enzyme in starch transformation of wheat endosperm. To improve wheat productivity under heat stress, the rice soluble starch synthase I, under the control of either a constitutive promoter or an endosperm-specific promoter, was expressed in wheat and the transgenic lines were monitored for expression and the effects on yield-related traits...
2018: In Vitro Cellular & Developmental Biology. Plant: Journal of the Tissue Culture Association
https://www.readbyqxmd.com/read/29779921/challenges-of-cold-chain-quality-for-routine-epi-in-south-west-burkina-faso-an-assessment-using-automated-temperature-recording-devices
#13
C Sow, C Sanou, C Medah, M Schlumberger, F Mireux, I Ouédraogo, S M Ouédraogo, E Betsem
BACKGROUND: Abnormal temperatures are a major issue for vaccines within the Expanded Program of Immunization in tropical climates. Prolonged exposure to temperatures outside the standard +2 °C/+8 °C range can impact vaccine potency. METHODS: The current study used automatic temperature recording devices (Testostore 171-1©) to monitor cold chain in remote areas of Western Burkina Faso. A series of 25 randomly selected health centers representing 33% of the existing 176 EPI facilities in Western Burkina Faso were prospectively assessed for eight months in 2015...
May 17, 2018: Vaccine
https://www.readbyqxmd.com/read/29779674/forecasting-respiratory-infectious-outbreaks-using-ed-based-syndromic-surveillance-for-febrile-ed-visits-in-a-metropolitan-city
#14
Tae Han Kim, Ki Jeong Hong, Sang Do Shin, Gwan Jin Park, Sungwan Kim, Nhayoung Hong
BACKGROUND: Monitoring and detecting sudden outbreaks of respiratory infectious disease is important. Emergency Department (ED)-based syndromic surveillance systems have been introduced for early detection of infectious outbreaks. The aim of this study was to develop and validate a forecasting model of respiratory infectious disease outbreaks based on a nationwide ED syndromic surveillance using daily number of emergency department visits with fever. METHODS: We measured the number of daily ED visits with body temperature ≥ 38...
May 10, 2018: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/29778871/linking-fish-tolerance-to-water-quality-criteria-for-the-assessment-of-environmental-flows-a-practical-method-for-streamflow-regulation-and-pollution-control
#15
Changsen Zhao, Shengtian Yang, Junguo Liu, Changming Liu, Fanghua Hao, Zhonggen Wang, Huitong Zhang, Jinxi Song, Simon M Mitrovic, Richard P Lim
The survival of aquatic biota in stream ecosystems depends on both water quantity and quality, and is particularly susceptible to degraded water quality in regulated rivers. Maintenance of environmental flows (e-flows) for aquatic biota with optimum water quantity and quality is essential for sustainable ecosystem services, especially in developing regions with insufficient stream monitoring of hydrology, water quality and aquatic biota. Few e-flow methods are available that closely link aquatic biota tolerances to pollutant concentrations in a simple and practical manner...
May 15, 2018: Water Research
https://www.readbyqxmd.com/read/29777098/stepwise-control-of-host-guest-interaction-using-a-coordination-polymer-gel
#16
Rahul Dev Mukhopadhyay, Gourab Das, Ayyappanpillai Ajayaghosh
Precise control of host-guest interaction as seen in biological processes is difficult to achieve with artificial systems. Herein we have exploited the thermodynamic benefits of a system in equilibrium to achieve controlled stepwise release and capture of cyclodextrin (guest) using a coordination polymer (Mg-CP) as the host and temperature as the stimulus. Since temperature is not a precision stimulus for artificial host-guest interaction, the present system is a distinct prototype that manifests temperature-controlled natural host-guest interaction...
May 18, 2018: Nature Communications
https://www.readbyqxmd.com/read/29776438/uninterrupted-monitoring-of-drug-effects-in-human-induced-pluripotent-stem-cell-derived-cardiomyocytes-with-bioluminescence-ca-2-microscopy
#17
Kazushi Suzuki, Takahito Onishi, Chieko Nakada, Shunsuke Takei, Matthew J Daniels, Masahiro Nakano, Tomoki Matsuda, Takeharu Nagai
OBJECTIVE: Cardiomyocytes derived from human-induced pluripotent stem cells are a powerful platform for high-throughput drug screening in vitro. However, current modalities for drug testing, such as electrophysiology and fluorescence imaging have inherent drawbacks. To circumvent these problems, we report the development of a bioluminescent Ca2+ indicator GmNL(Ca2+ ), and its application in a customized microscope for high-throughput drug screening. RESULTS: GmNL(Ca2+ ) gives a 140% signal change with Ca2+ , and can image drug-induced changes of Ca2+ dynamics in cultured cells...
May 18, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29775421/probability-of-heat-intolerance-standardized-interpretation-of-heat-tolerance-testing-results-versus-specialist-judgment
#18
Haggai Schermann, Erin Craig, Einat Yanovich, Itay Ketko, Gary Kalmanovich, Ran Yanovich
CONTEXT:   The heat-tolerance test (HTT) is a screening tool for secondary prevention of exertional heat illness by the Israel Defense Forces. To discern participant tolerance, recruits are exposed to intermediate environmental and exercise stresses, and their physiological responses, core temperature, and heart rate are monitored. When their physiological measures rise at a higher rate or exceed the upper levels of absolute values compared with other participants, heat intolerance (HI) is diagnosed...
April 2018: Journal of Athletic Training
https://www.readbyqxmd.com/read/29774894/value-of-diffusion-weighted-imaging-for-monitoring-tissue-change-during-magnetic-resonance-guided-high-intensity-focused-ultrasound-therapy-in-bone-applications-an-ex-vivo-study
#19
Sharon L Giles, Jessica M Winfield, David J Collins, Ian Rivens, John Civale, Gail R Ter Haar, Nandita M deSouza
Background: Magnetic resonance (MR)-guided high-intensity focused ultrasound (HIFU) can palliate metastatic bone pain by periosteal neurolysis. We investigated the value of diffusion-weighted imaging (DWI) for monitoring soft tissue changes adjacent to bone during MR-guided HIFU. We evaluated the repeatability of the apparent diffusion coefficient (ADC) measurement, the temporal evolution of ADC change after sonication, and its relationship with thermal parameters. Methods: Ex-vivo experiments in lamb legs ( n = 8) were performed on a Sonalleve MR-guided HIFU system...
2018: European radiology experimental
https://www.readbyqxmd.com/read/29773927/influence-of-meteorological-factors-on-the-level-and-characteristics-of-culturable-bacteria-in-the-air-in-gliwice-upper-silesia-poland
#20
Ewa Brągoszewska, Józef S Pastuszka
Numerous studies have focused on occupational and indoor environments because people spend more than 90% of their time in them. Nevertheless, air is the main source of bacteria in indoors, and outdoor exposure is also crucial. Worldwide studies have indicated that bacterial concentrations vary among different types of outdoor environments, with considerable seasonal variations as well. Conducting comprehensive monitoring of atmospheric aerosol concentrations is very important not only for environmental management but also for the assessment of the health impacts of air pollution...
2018: Aerobiologia
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