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https://www.readbyqxmd.com/read/28107558/predictors-of-summertime-heat-index-levels-in-new-york-city-apartments
#1
Ashlinn Quinn, Patrick Kinney, Jeffrey Shaman
BACKGROUND: During heat waves, fatal overexposure to heat most often occurs at home. It is not known how factors such as building size, floor level, and different types of air conditioning (AC) contribute to excess indoor heat. METHODS: We monitored indoor temperature and humidity in 36 apartments in New York City during summers 2014 and 2015, and used these values to calculate the indoor heat index (HI). We investigated the role of AC type and building-level factors on indoor HI using multilevel regression models...
January 20, 2017: Indoor Air
https://www.readbyqxmd.com/read/28107301/implementation-of-a-pediatric-orthopaedic-bundle-to-reduce-surgical-site-infections
#2
Jan Schriefer, James Sanders, Julie Michels, Kori Wolcott, Connor Ruddy, Jenna Hanson
Surgical site infections (SSIs) cost an estimated $27,288 per case. An analysis of the National Surgical Quality Improvement Program data at the University of Rochester Medical Center suggested that rates of SSIs could be lowered in comparison with both peers and baseline. The aim of this study was to reduce the number of SSIs to zero through the implementation of a "bundle" or a combination of practices. Meetings were held with the multidisciplinary care team that includes surgeons and staff from pediatric pharmacy, pediatric infectious diseases, anesthesia, and nursing to create a care bundle for all pediatric orthopaedic surgery patients...
January 2017: Orthopaedic Nursing
https://www.readbyqxmd.com/read/28106834/heat-stress-decreases-levels-of-nutrient-uptake-and-assimilation-proteins-in-tomato-roots
#3
Anju Giri, Scott Heckathorn, Sasmita Mishra, Charles Krause
Global warming will increase root heat stress, which is already common under certain conditions. Effects of heat stress on root nutrient uptake have rarely been examined in intact plants, but the limited results indicate that heat stress will decrease it; no studies have examined heat-stress effects on the concentration of nutrient-uptake proteins. We grew Solanum lycopersicum (tomato) at 25 °C/20 °C (day/night) and then transferred some plants for six days to 35 °C /30 °C (moderate heat) or 42 °C/37 °C (severe heat) (maximum root temperature = 32 °C or 39 °C, respectively); plants were then moved back to control conditions for seven days to monitor recovery...
January 19, 2017: Plants (Basel, Switzerland)
https://www.readbyqxmd.com/read/28106832/plaimos-a-remote-mobile-healthcare-platform-to-monitor-cardiovascular-and-respiratory-variables
#4
Ramses Miramontes, Raúl Aquino, Arturo Flores, Guillermo Rodríguez, Rafael Anguiano, Arturo Ríos, Arthur Edwards
The number of elderly and chronically ill patients has grown significantly over the past few decades as life expectancy has increased worldwide, leading to increased demands on the health care system and significantly taxing traditional health care practices. Consequently, there is an urgent need to use technology to innovate and more constantly and intensely monitor, report and analyze critical patient physiological parameters beyond conventional clinical settings in a more efficient and cost effective manner...
January 19, 2017: Sensors
https://www.readbyqxmd.com/read/28106399/-1-h-nmr-shows-slow-phospholipid-flip-flop-in-gel-and-fluid-bilayers
#5
Drew Marquardt, Frederick A Heberle, Tatiana Miti, Barbara Eicher, Erwin London, John Katsaras, Georg Pabst
We measured the transbilayer diffusion of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) in large unilamellar vesicles in both the gel (Lβ') and fluid (Lα) phases. The choline resonance of headgroup-protiated DPPC exchanged into the outer leaflet of headgroup-deuterated DPPC-d13 vesicles was monitored using (1)H NMR spectroscopy coupled with the addition of a paramagnetic shift reagent. This allowed us to distinguish between the inner and outer leaflet DPPC, and determine the flip-flop rate as a function of temperature...
January 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28106351/real-time-ph-monitoring-of-industrially-relevant-enzymatic-reactions-in-a-microfluidic-side-entry-reactor-%C3%AE-ser-shows-potential-for-ph-control
#6
Pia Gruber, Marco P C Marques, Philipp Sulzer, Roland Wohlgemuth, Torsten Mayr, Frank Baganz, Nicolas Szita
Monitoring and control of pH is essential for the control of reaction conditions and reaction progress for any biocatalytic or biotechnological process. Microfluidic enzymatic reactors are increasingly proposed for process development, however typically lack instrumentation, such as pH monitoring. We present a microfluidic side-entry reactor (μSER) and demonstrate for the first time real-time pH monitoring of the progression of an enzymatic reaction in a microfluidic reactor as a first step towards achieving pH control...
January 20, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28106340/gas-chromatography-with-mass-spectrometry-for-the-determination-of-phthalates-preconcentrated-by-microextraction-based-on-an-ionic-liquid
#7
Juan Ignacio Cacho, Natalia Campillo, Pilar Viñas, Manuel Hernández-Córdoba
A new procedure is proposed for the analysis of migration test solutions obtained from plastic bottles used in the packaging of edible oils. Ultrasound assisted emulsification microextraction with ionic liquids was applied for the preconcentration of six phthalate esters: dimethyl-, diethyl-, di-n-butyl, n-butylbenzyl, di-2-ethylhexyl and di-n-octylphthalate. The enriched ionic liquid was directly analyzed by gas chromatography and mass spectrometry using direct insert microvial thermal desorption. The different factors affecting the microextraction efficiency, like volume of the extracting phase (30 μL of the ionic liquid) and ultrasounds application time (25 s); and the thermal desorption step, such as desorption temperature and time, and gas flow rate, were studied...
January 20, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28106279/a-rapid-electrochemical-monitoring-platform-for-sensitive-determination-of-thiamethoxam-based-on-%C3%AE-cyclodextrin-graphene-composite
#8
XingChen Zhai, Hua Zhang, Min Zhang, Xin Yang, Cheng Gu, GuoPeng Zhou, Haitian Zhao, Zhenyu Wang, Aijun Dong, Jing Wang
A rapid monitoring platform for sensitive voltammetric detection of thiamethoxam (TMX) residues was reported in the present study. The β-cyclodextrin-reduced graphene oxide composite (β-CD-rGO) was used as a reinforcing material in electrochemical determination of TMX. Compared with bare glassy carbon electrode (GCE), the reduction peak currents of TMX at rGO/GCE and β-CD-rGO/GCE were increased by 70 and 124 folds, respectively. The experimental conditions influencing voltammetric determination of TMX, such as the amount of β-CD-rGO, solution pH, temperature and time on accumulation, were optimized...
January 20, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28106167/effects-of-climatic-variables-on-weight-loss-a-global-analysis
#9
Morena Ustulin, Changwon Keum, Junghoon Woo, Jeong-Taek Woo, Sang Youl Rhee
Several studies have analyzed the effects of weather on factors associated with weight loss. In this study, we directly analyzed the effect of weather on intentional weight loss using global-scale data provided by smartphone applications. Through Weather Underground API and the Noom Coach application, we extracted information on weather and body weight for each user located in each of several geographic areas on all login days. We identified meteorological information (pressure, precipitation, wind speed, dew point, and temperature) and self-monitored body weight data simultaneously...
January 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28105843/adaptive-pcr-based-on-hybridization-sensing-of-mirror-image-l-dna
#10
Nicholas M Adams, William E Gabella, Austin N Hardcastle, Frederick R Haselton
Polymerase chain reaction (PCR) is dependent on two key hybridization events during each cycle of amplification, primer annealing and product melting. To ensure that these hybridization events occur, current PCR approaches rely on temperature set points and reaction contents that are optimized and maintained using rigid thermal cycling programs and stringent sample preparation procedures. This report describes a fundamentally simpler and more robust PCR design that dynamically controls thermal cycling by more directly monitoring the two key hybridization events during the reaction...
January 3, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28105596/monitoring-nutrients-fate-after-digestate-spreading-in-a-short-rotation-buffer-area
#11
Nicolò Colombani, Bruno Boz, Bruna Gumiero, Micòl Mastrocicco
One of the main sources of reactive nitrogen pollution is animal manure. The disposal of digestate (material remaining after the anaerobic digestion of a biodegradable feedstock) in agricultural soils could solve both the problems of soil fertilization and waste removal, but the fate of digestate in the environment must be assessed carefully before its massive utilization. To investigate whether digestate could be safely employed as a soil fertilizer, an agricultural field located in Monastier di Treviso (Northern Italy) and characterized by the presence of low hydraulic conductivity clay soils, was selected to be amended with bovine digestate...
January 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28102691/thermodynamic-and-kinetic-stabilities-of-g-quadruplexes-in-apolar-solvents
#12
Elham Fadaei, Miguel Martín-Arroyo, Mohsen Tafazzoli, David González-Rodríguez
In contrast to more polar media, G-quadruplex assembly reveals remarkably high thermodynamic and kinetic stability in toluene solutions. Depending on the substituents installed at the lipophilic guanosine, either a dodecamer or an octamer complex is formed in the presence of K(+) or Na(+) salts that resist conditions of high dilution and elevated temperatures without exhibiting significant dissociation. Moreover, kinetic exchange between complexed and uncomplexed G is slow enough in NMR to monitor G-quadruplex formation along a day time scale...
January 19, 2017: Organic Letters
https://www.readbyqxmd.com/read/28102317/time-resolved-neutron-scattering-provides-new-insight-into-protein-substrate-processing-by-a-aaa-unfoldase
#13
Ziad Ibrahim, Anne Martel, Martine Moulin, Henry S Kim, Michael Härtlein, Bruno Franzetti, Frank Gabel
We present a combination of small-angle neutron scattering, deuterium labelling and contrast variation, temperature activation and fluorescence spectroscopy as a novel approach to obtain time-resolved, structural data individually from macromolecular complexes and their substrates during active biochemical reactions. The approach allowed us to monitor the mechanical unfolding of a green fluorescent protein model substrate by the archaeal AAA+ PAN unfoldase on the sub-minute time scale. Concomitant with the unfolding of its substrate, the PAN complex underwent an energy-dependent transition from a relaxed to a contracted conformation, followed by a slower expansion to its initial state at the end of the reaction...
January 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28102286/chelerythrine-down-regulates-expression-of-vegfa-bcl2-and-kras-by-arresting-g-quadruplex-structures-at-their-promoter-regions
#14
Jagannath Jana, Soma Mondal, Payel Bhattacharjee, Pallabi Sengupta, Tanaya Roychowdhury, Pranay Saha, Pallob Kundu, Subhrangsu Chatterjee
A putative anticancer plant alkaloid, Chelerythrine binds to G-quadruplexes at promoters of VEGFA, BCL2 and KRAS genes and down regulates their expression. The association of Chelerythrine to G-quadruplex at the promoters of these oncogenes were monitored using UV absorption spectroscopy, fluorescence anisotropy, circular dichroism spectroscopy, CD melting, isothermal titration calorimetry, molecular dynamics simulation and quantitative RT-PCR technique. The pronounced hypochromism accompanied by red shifts in UV absorption spectroscopy in conjunction with ethidium bromide displacement assay indicates end stacking mode of interaction of Chelerythrine with the corresponding G-quadruplex structures...
January 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28101802/cold-pcr-technologies-in-the-area-of-personalized-medicine-methodology-and-applications
#15
REVIEW
Florence Mauger, Alexandre How-Kit, Jörg Tost
Somatic mutations bear great promise for use as biomarkers for personalized medicine, but are often present only in low abundance in biological material and are therefore difficult to detect. Many assays for mutation analysis in cancer-related genes (hotspots) have been developed to improve diagnosis, prognosis, prediction of drug resistance, and monitoring of the response to treatment. Two major approaches have been developed: mutation-specific amplification methods and methods that enrich and detect mutations without prior knowledge on the exact location and identity of the mutation...
January 18, 2017: Molecular Diagnosis & Therapy
https://www.readbyqxmd.com/read/28101566/effect-of-physical-activity-in-the-first-five-days-after-cardiac-surgery
#16
Sean F Mungovan, Preetraj Singh, Gregory C Gass, Neil A Smart, Andrew D Hirschhorn
OBJECTIVES: To quantify physiotherapist-supervised and independent physical activity undertaken from the first to the fifth day after cardiac surgery (POD1 to POD5), and to relate the amount of physical activity undertaken with hospital stay and postoperative physiological functional capacity on POD6. METHODS: Physiotherapist-supervised and independent physical activity were monitored in 83 adult patients undergoing cardiac surgery, using a bi-axial accelerometer and skin sensors that measured, galvanic skin response and body temperature...
January 19, 2017: Journal of Rehabilitation Medicine
https://www.readbyqxmd.com/read/28100807/cost-minimisation-analysis-of-thermometry-in-two-different-hospital-systems
#17
Kenneth Hayes, Amanda Shepard, August Cesarec, Robert Likić
INTRODUCTION: Temperature monitoring can be accomplished by various methods, including oral (OT), rectal (RT), axillary (AT), tympanic membrane (TMT) and temporal artery (TAT) thermometry, with varying amounts of cost incurred by healthcare systems. METHODS: The potential thermometry cost savings in two hospital systems-University Hospital Centre Zagreb (UHCZ), which uses TMT (device Covidien Genius 2) and University of Michigan Hospitals (UMH), which relies on OT, RT and AT (device Welch Allyn suretemp plus 692)-were analysed to evaluate institution-wide TAT (device Exergen TAT-5000) implementation...
January 18, 2017: Postgraduate Medical Journal
https://www.readbyqxmd.com/read/28099965/pilot-test-of-a-new-personal-health-system-integrating-environmental-and-wearable-sensors-for-telemonitoring-and-care-of-elderly-people-at-home-smarta-project
#18
Lucia Pigini, Gabriele Bovi, Claudia Panzarino, Valerio Gower, Maurizio Ferratini, Giuseppe Andreoni, Roberto Sassi, Massimo W Rivolta, Maurizio Ferrarin
BACKGROUND: The increase in life expectancy is accompanied by a growing number of elderly subjects affected by chronic comorbidities, a health issue which also implies important socioeconomic consequences. Shifting from hospital or community dwelling care towards a home personalized healthcare paradigm would promote active aging with a better quality of life, along with a reduction in healthcare-related costs. OBJECTIVE: The aim of the SMARTA project was to develop and test an innovative personal health system integrating standard sensors as well as innovative wearable and environmental sensors to allow home telemonitoring of vital parameters and detection of anomalies in daily activities, thus supporting active aging through remote healthcare...
January 19, 2017: Gerontology
https://www.readbyqxmd.com/read/28099549/warming-up-for-mechanosynthesis-temperature-development-in-ball-mills-during-synthesis
#19
Hannes Kulla, Manuel Wilke, Franziska Fischer, Mathias Röllig, Christiane Maierhofer, Franziska Emmerling
We present a first direct measurement of the temperature during milling combined with in situ Raman spectroscopy monitoring. The data reveal a low temperature increase due to the mechanical impact and clear temperature increases as a consequence of the reaction heat. Based on the data, temperature rises as postulated in the magma plasma and hot spot theory can be excluded for soft matter milling syntheses.
January 18, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/28099162/thermography-based-blood-flow-imaging-in-human-skin-of-the-hands-and-feet-a-spectral-filtering-approach
#20
A A Sagaidachnyi, A V Fomin, D A Usanov, A V Skripal
The determination of the relationship between skin blood flow and skin temperature dynamics is the main problem in thermography-based blood flow imaging. Oscillations in skin blood flow are the source of thermal waves propagating from micro-vessels toward the skin's surface, as assumed in this study. This hypothesis allows us to use equations for the attenuation and dispersion of thermal waves for converting the temperature signal into the blood flow signal, and vice versa. We developed a spectral filtering approach (SFA), which is a new technique for thermography-based blood flow imaging...
January 18, 2017: Physiological Measurement
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