Chen-Hui Shen, Min Tang, Xiao-Fei Li, Li Zhu, Wei Li, Pan Deng, Qing Zhai, Gang Wu, Xiao-Hong Yan
Mylabris sibirica is a hypermetamorphic insect whose adults feed on oilseed rape. However, due to a shortage of effective and appropriate endogenous references, studies on molecular functional genes in Mylabris sibirica , have been tremendously limited. In this study, ten internal reference genes ( ACT , ARF1 , AK , EF1α , GAPDH , α-TUB , RPL6 , RPL13 , RPS3 and RPS18 ) were tested and assessed under four selected treatments including adult ages, adult tissues, temperatures, and sex by RT-qPCR based on five methods (Ct value, geNorm, NormFinder, BestKeeper and RefFinder)...
2024: Frontiers in Physiology