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https://www.readbyqxmd.com/read/29151156/deep-rnaseq-indicates-protective-mechanisms-of-cold-tolerant-indica-rice-plants-during-early-vegetative-stage
#1
Raul Antonio Sperotto, Artur Teixeira de Araújo Junior, Janete Mariza Adamski, Denise Cargnelutti, Felipe Klein Ricachenevsky, Ben-Hur Neves de Oliveira, Renata Pereira da Cruz, Rinaldo Pires Dos Santos, Leila Picolli da Silva, Janette Palma Fett
Cold-tolerance in rice may be related to increased cellulose deposition in the cell wall, membrane fatty acids unsaturation and differential expression of several newly identified genes. Low temperature exposure during early vegetative stages limits rice plant's growth and development. Most genes previously related to cold tolerance in rice are from the japonica subspecies. To help clarify the mechanisms that regulate cold tolerance in young indica rice plants, comparative transcriptome analysis of 6 h cold-treated (10 °C) leaves from two genotypes, cold-tolerant (CT) and cold-sensitive (CS), was performed...
November 18, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/29150945/development-of-an-in-vivo-mouse-model-of-discogenic-low-back-pain
#2
Changgui Shi, Vaskar Das, Xin Li, Ranjan Kc, Sujun Qiu, InSug O-Sullivan, Richard L Ripper, Jeffrey S Kroin, Fackson Mwale, Atiyayein A Wallace, Bingqian Zhu, Lan Zhao, Andre J van Wijnen, Mingliang Ji, Jun Lu, Gina Votta-Velis, Wen Yuan, Hee-Jeong Im
Discogenic Low back pain (DLBP) is extremely common and costly. Effective treatments are lacking due to DLBP's unknown pathogenesis. Currently, there are no in vivo mouse models of DLBP, which restricts research in this field. The aim of this study was to establish a reliable DLBP model in mouse that captures the pathological changes in the disc and allows longitudinal pain testing. The model was generated by puncturing the mouse lumbar discs (L4/5, L5/6, and L6/S1) and removing the nucleus pulposus using a microscalpel under the microscope...
November 18, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29150823/metabolic-dynamics-and-physiological-adaptation-of-panax-ginseng-during-development
#3
Yu-Jin Kim, Sung Chul Joo, Jianxin Shi, Chaoyang Hu, Sheng Quan, Jianping Hu, Johan Sukweenadhi, Padmanaban Mohanan, Deok-Chun Yang, Dabing Zhang
The dynamics of metabolites from leaves to roots of Panax ginseng during development has revealed the tissue-specific and year-specific metabolic networks. Being an essential Oriental medicinal plant, ginseng (Panax ginseng Meyer) is a slow-growing perennial herb-accumulating pharmaceutically active metabolites such as ginsenosides in roots during growth. However, little is known about how ginseng plants survive in the harsh environments such as winter cold and summer heat for a longer period and accumulates those active metabolites as the plant grows...
November 17, 2017: Plant Cell Reports
https://www.readbyqxmd.com/read/29150789/radial-pulse-and-electrocardiography-modulation-by-mild-thermal-stresses-applied-to-feet-an-exploratory-study-with-randomized-crossover-design
#4
Jang-Han Bae, Boncho Ku, Young Ju Jeon, Hyunho Kim, Jihye Kim, Haebeom Lee, Jong Yeol Kim, Jaeuk U Kim
OBJECTIVE: To investigate the changes in radial pulse induced by thermal stresses (TSs). METHODS: Sixty subjects were enrolled. Using an open-label, 2×2 crossover randomization design, both feet of each subject were immersed in 15 °C water for cold stress (CS) and in 40 °C water for heat stress (HS) for 5 min each. Radial pulse, respiration and electrocardiogram (ECG) signals were recorded before, during and immediately after the TSs. RESULTS: The analysis of heart rate variability revealed that CS increased the low-frequency (LF) and high-frequency (HF) components (P <0...
November 17, 2017: Chinese Journal of Integrative Medicine
https://www.readbyqxmd.com/read/29150716/slow-life-histories-in-lizards-living-in-the-highlands-of-the-andes-mountains
#5
Jorgelina M Boretto, Facundo Cabezas-Cartes, Nora R Ibargüengoytía
In the highlands of the Andes, lizards must balance precisely the allocation of energy for growth and reproduction to ensure their survival. We studied the individuals' age, growth rates, age at sexual maturity, and maximum life span of the viviparous lizard Phymaturus antofagastensis, endemic of cold and harsh environments at high altitudes in the Andes Mountains of Catamarca province, Argentina. We also estimated key life history parameters like reproductive effort, lifetime reproductive effort, net reproductive rate, and relative reproductive time in P...
November 17, 2017: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/29150627/high-efficiency-backward-four-wave-mixing-by-quantum-interference
#6
Zi-Yu Liu, Jian-Ting Xiao, Jia-Kang Lin, Jun-Jie Wu, Jz-Yuan Juo, Chin-Yao Cheng, Yong-Fan Chen
Electromagnetically-induced-transparency-based four-wave mixing (FWM) in a resonant four-level double-Λ system has a maximum conversion efficiency (CE) of 25% due to spontaneous emission. Herein, we demonstrate that spontaneous emission can be considerably suppressed by arranging the applied laser beams in a backward configuration. With the backward double-Λ FWM scheme, we observe a CE of 63% in cold rubidium atoms with an optical depth (OD) of 48. To the best of our knowledge, this is the first observation of a CE exceeding the conversion limit in resonant FWM processes...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29150611/uplift-of-the-central-transantarctic-mountains
#7
Phil Wannamaker, Graham Hill, John Stodt, Virginie Maris, Yasuo Ogawa, Kate Selway, Goran Boren, Edward Bertrand, Daniel Uhlmann, Bridget Ayling, A Marie Green, Daniel Feucht
The Transantarctic Mountains (TAM) are the world's longest rift shoulder but the source of their high elevation is enigmatic. To discriminate the importance of mechanical vs. thermal sources of support, a 550 km-long transect of magnetotelluric geophysical soundings spanning the central TAM was acquired. These data reveal a lithosphere of high electrical resistivity to at least 150 km depth, implying a cold stable state well into the upper mantle. Here we find that the central TAM most likely are elevated by a non-thermal, flexural cantilever mechanism which is perhaps the most clearly expressed example anywhere...
November 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/29149974/liver-transplantation-with-old-grafts-a-ten-year-experience
#8
S Roullet, M Defaye, A Quinart, J-P Adam, L Chiche, C Laurent, M Neau-Cransac
BACKGROUND: The persistent scarcity of donors has prompted liver transplantation teams to find solutions for increasing graft availability. We report our experience of liver transplantations performed with grafts from older donors, specifically over 70 and 80 years old. PATIENTS AND METHODS: We analyzed our prospectively maintained single-center database from January 1, 2005, to December 31, 2014, with 380 liver transplantations performed in 354 patients. Six groups were composed according to donor age: <40 (n = 84), 40 to 49 (n = 67), from 50 to 59 (n = 62), from 60 to 69 (n = 76), from 70 to 79 (n = 64), and ≥80 years (n = 27)...
November 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29149972/right-extended-split-liver-transplantation-compared-with-whole-liver-transplantation-lessons-learned-at-a-single-center-in-latin-america-results-from-a-match-case-control-study
#9
S E Gambaro, P Romero, N Pedraza, L Moulin, S Yantorno, D Ramisch, C Rumbo, P Barros-Schelotto, V Descalzi, G E Gondolesi
BACKGROUND: Despite the progressively increasing gap between patients waiting for liver transplant under the Model for End-stage Liver Disease MELD system and the availability of deceased donor organs, the use of right extended split liver grafts (RESLG) has not been accepted by all centers. In this study, we compared the results obtained using RESLG vs a group of matched whole liver graft (WLG) recipients at a single center in Latin America. METHODS: A single-center retrospective review performed between August 2009 and December 2015...
November 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29149960/real-renal-function-after-renal-autotransplantation-through-the-analysis-of-solitary-kidney-autotransplantation-cases
#10
J G Gwon, Y H Kim, D J Han
INTRODUCTION: Previous studies of the safety of renal autotransplantation (RAT) have been limited by a lack of evidence. Because of the influence of the opposite kidney, it is difficult to evaluate actual renal function. This study evaluated the actual results of RAT by collecting only cases involving a solitary kidney. METHODS: Between September 1, 1999, and November 30, 2015, 9 RAT procedures were performed in 9 patients with a solitary kidney. We retrospectively evaluated medical data collected prospectively...
November 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/29149876/impact-of-donor-graft-quality-on-deep-anterior-lamellar-keratoplasty-dalk
#11
Friederike Schaub, Philip Enders, Werner Adler, Björn O Bachmann, Claus Cursiefen, Ludwig M Heindl
BACKGROUND: To evaluate main features of donor tissue that may influence clinical outcome or complication rate after deep anterior lamellar keratoplasty (DALK). METHODS: Donor tissue parameters of 84 consecutive corneal donor grafts used for big-bubble DALK surgery between June 2011 and December 2014 in 84 eyes of 84 patients with disorders of anterior corneal stroma were correlated to clinical outcome parameters of recipient eyes 12 months after surgery and 3 months after total suture removal...
November 17, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/29149744/bacterial-community-structure-along-the-subtidal-sandy-sediment-belt-of-a-high-arctic-fjord-kongsfjorden-svalbard-islands
#12
Conte Antonella, Papale Maria, Amalfitano Stefano, Mikkonen Anu, Rizzo Carmen, De Domenico Emilio, Michaud Luigi, Lo Giudice Angelina
Open fjords are subject to contrasting environmental conditions, owing to meltwater glacial inputs, terrestrial runoff, and marine water mass exchanges, which are exacerbated by anthropogenic and climate perturbations. Following a slope-dependent water circulation, the subtidal sandy sediment belt regulates the convergent transport of nutrients downward the fjord depths, and the effective entrapment of suspended particles and microorganisms. In this study, we aimed at testing how glacial and seawater inputs may influence the bacterial community structure of subtidal sand deposits in the Kongsfjorden...
November 14, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/29149645/xylella-fastidiosa-induces-differential-expression-of-lignification-related-genes-and-lignin-accumulation-in-tolerant-olive-trees-cv-leccino
#13
Erika Sabella, Andrea Luvisi, Alessio Aprile, Carmine Negro, Marzia Vergine, Francesca Nicolì, Antonio Miceli, Luigi De Bellis
Recently, Xylella fastidiosa was reported in Italy, associated with the "Olive Quick Decline Syndrome". The cv. Leccino exhibits an evident tolerance with a slow disease progression compared with the other cultivars. Between the mechanisms proposed to explain the putative tolerance of some hosts to X. fastidiosa diseases, lignin deposition plays an important role. Analysis of phenolic compounds in healthy and infected Leccino and Cellina di Nardò leaves showed, in the two cultivars, a reduction of hydroxytyrosol glucoside (usually associated with drought and cold stress) and, only in Leccino, an increase of quinic acid, precursor of lignin...
November 10, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/29149596/rational-design-of-evolutionarily-stable-microbial-kill-switches
#14
Finn Stirling, Lisa Bitzan, Samuel O'Keefe, Elizabeth Redfield, John W K Oliver, Jeffrey Way, Pamela A Silver
The evolutionary stability of synthetic genetic circuits is key to both the understanding and application of genetic control elements. One useful but challenging situation is a switch between life and death depending on environment. Here are presented "essentializer" and "cryodeath" circuits, which act as kill switches in Escherichia coli. The essentializer element induces cell death upon the loss of a bi-stable cI/Cro memory switch. Cryodeath makes use of a cold-inducible promoter to express a toxin. We employ rational design and a toxin/antitoxin titering approach to produce and screen a small library of potential constructs, in order to select for constructs that are evolutionarily stable...
November 16, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29149492/effects-of-tao-hong-si-wu-decoction-on-acute-blood-stasis-in-rats-based-on-a-lc-q-tof-ms-metabolomics-and-network-approach
#15
Qi Ma, Peng-Ling Li, Yong-Li Hua, Peng Ji, Wan-Ling Yao, Xiao-Song Zhang, Li-Jia Zhong, Yan-Ming Wei
A novel approach using metabolomics coupled with a metabolic network was used to investigate the effects of THSWD on the rat model of acute blood stasis syndrome. Acute blood stasis syndrome was induced by placing the rats in ice-cold water following two injections with epinephrine. The hemorheological indicators (WBV and PV), and the blood coagulation indicators (TT, PT, APTT, and FIB) were detected. The nonparametric univariate method and multivariate statistical analysis were performed for determining the potential biomarkers...
November 17, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29149482/melatonin-alleviates-low-ps-i-limited-carbon-assimilation-under-elevated-co2-and-enhances-the-cold-tolerance-of-offspring-in-chlorophyll-b-deficient-mutant-wheat
#16
Xiangnan Li, Marian Brestic, Dun-Xian Tan, Marek Zivcak, Xiancan Zhu, Shengqun Liu, Fengbin Song, Russel J Reiter, Fulai Liu
Melatonin is involved in the regulation of carbohydrate metabolism and induction of cold tolerance in plants. The objective of this study was to investigate the roles of melatonin in modulation of carbon assimilation of wild type wheat and the Chl b-deficient mutant ANK32B in response to elevated CO2 concentration ([CO2 ]) and the transgenerational effects of application of exogenous melatonin (hereafter identified as melatonin priming) on the cold tolerance in offspring. The results showed that the melatonin priming enhanced the carbon assimilation in ANK32B under elevated [CO2 ], via boosting the activities of ATPase and sucrose synthesis and maintaining a relatively higher level of total chlorophyll concentration in leaves...
November 17, 2017: Journal of Pineal Research
https://www.readbyqxmd.com/read/29149095/proton-nuclear-magnetic-resonance-%C3%A2-h-nmr-based-metabolomic-evaluation-of-human-renal-allografts-from-donations-after-circulatory-death
#17
Zijie Wang, Haiwei Yang, Chunchun Zhao, Jifu Wei, Junsong Wang, Zhijian Han, Jun Tao, Zhen Xu, Xiaobin Ju, Ruoyun Tan, Min Gu
BACKGROUND Delayed graft function (DGF) is a common complication that impairs allograft function after kidney transplantation. However, the mechanism of DGF remains unclear. Nuclear magnetic resonance (NMR)-based analysis has been widely used in recent times to assess changes in metabolite levels. MATERIAL AND METHODS Samples of perfusate from allografts donated after circulatory death were collected prior to transplantation, during static cold storage. ¹H-NMR-based metabolomics combined with the statistical methods, orthogonal partial least-squares discriminant analysis (OPLS-DA), and principle-component analysis (PCA), were employed to test different levels of metabolites between the allografts that exhibited DGF and those that exhibited immediate graft function (IGF)...
November 17, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29148978/antidiabetic-and-in-vitro-antioxidant-effects-of-hydromethanol-extract-of-paullinia-pinnata-root-bark-in-alloxan-induced-diabetic-rat
#18
Onoja Samuel Okwudili, Nwachukwu Glory Chimaobi, Ezeja Maxwell Ikechukwu, Omeh Yusuf Ndukaku
Background The study evaluated phytochemical composition, antidiabetic, oral glucose tolerance test and in vitro antioxidant activities of hydromethanol extract of Paullinia pinnata root bark. Methods Cold maceration method was used in extract preparation and scavenging of 2,2-diphenyl-1-picrylhydrazyl radicals was used to evaluate antioxidant properties of the extract. Diabetes was induced with alloxan at the dose of 160 mg/kg. The antidiabetic activity of the extract was tested at doses of 50, 100 and 200 mg/kg, and glibenclamide was used as reference drug...
November 17, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/29148825/on-the-growth-and-detectability-of-land-plants-on-habitable-planets-around-m-dwarfs
#19
Duo Cui, Feng Tian, Yuwei Wang, Changshen Li, Chaoqing Yu, Le Yu
One signature of life on Earth is the vegetation red edge (VRE) feature of land plants, a dramatic change of reflectivity at wavelength near 0.7 μm. Potentially habitable planets around M dwarfs are tidally locked, which can limit the distribution of land plants. In this study, we used a biogeochemical model to investigate the distribution of land plants on potentially habitable planets around M dwarfs driven by climate data produced in a general circulation model (GCM). When considering the effects of clouds, the observation time needed for VRE detection on nearby p = 1 exoplanets around nearby M dwarfs is on the order of days using a 25 m(2) telescope if a large continent faces Earth during observations...
November 17, 2017: Astrobiology
https://www.readbyqxmd.com/read/29148824/biobanking-of-fresh-frozen-cancer-tissue-rna-is-stable-independent-of-tissue-type-with-less-than-1-hour-of-cold-ischemia
#20
Sang Yong Song, Jonghyun Jun, Miyeon Park, Seo Kyu Park, Wonju Choi, Kyunghee Park, Kee-Taek Jang, Myoyong Lee
BACKGROUND: The effects of preanalytical variables in tissue processing and storage periods on RNA quality of tissues have been well documented in each type of cancer. However, few studies have been performed on a comparative assessment of the impacts across different cancer tissues, even though it is well known that RNase activity is highly variable in various tissue types and RNase-rich tissues have been found to yield low-quality RNA. METHODS: We investigated the impacts of cold ischemia times and long-term storage on RNA integrity in various types of cancer tissue, which had been fresh-frozen and collected at the Samsung Medical Center Biobank...
November 17, 2017: Biopreservation and Biobanking
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