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https://www.readbyqxmd.com/read/28945039/-effects-of-xuesaitong-injection-on-inflammation-induced-by-ogd-reoxygenation-in-bv2-microglia
#1
Qian Hong, Shi Wang, Chang-Xiu Chen, Yu Zhang, Yang Wang, Yuan Pan, Zeng-Chun Ma
The present study aims to investigate the effects of Xuesaitong (XST)injection on inflammation induced by OGD/reoxygenation in BV2 microglia cell and explore the underlying mechanisms.The effects of XST injection were evaluated in terms of cell viability, secretion of TNF-α, IL-1β, IL-6 and IL-10 into culture media, protein expression of p-ERK1/2, p-JNK and p-p38, and nuclear translocation of NF-κB p65. The results showed that XST injection significantly increased cell viability, suppressed release of TNF-α, IL-1β, IL-6 and IL-10 and down-regulated p-JNK1/2 and p-p38 MAPK expression in BV2 microglia cells induced by OGD/R injury, whereas it had no effect on p-ERK1/2 expression...
January 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28945007/nonmineralized-and-mineralized-collagen-scaffolds-induce-differential-osteogenic-signaling-pathways-in-human-mesenchymal-stem-cells
#2
Qi Zhou, Xiaoyan Ren, David Bischoff, Daniel W Weisgerber, Dean T Yamaguchi, Timothy A Miller, Brendan A C Harley, Justine C Lee
The instructive capabilities of extracellular matrix components in progenitor cell differentiation have recently generated significant interest in the development of bioinspired materials for regenerative applications. Previously, a correlation was described between the osteogenic capabilities of nanoparticulate mineralized collagen glycosaminoglycan scaffolds (MC-GAG) and an autogenous activation of small mothers against decapentaplegic ( Smad1/5) in the canonical bone morphogenetic protein receptor (BMPR) pathway with a diminished extracellular signal regulated kinase 1/2 (ERK1/2) activation when compared to nonmineralized collagen glycosaminoglycan scaffolds (Col-GAG)...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28944996/negative-pressure-wound-therapy-promotes-muscle-derived-stem-cell-osteogenic-differentiation-through-mapk-pathway
#3
Hong Liu, Xun Zheng, Liang Chen, Chao Jian, Xiang Hu, Yong Zhao, Zonghuan Li, Aixi Yu
Negative pressure wound therapy (NPWT) has been revealed to be effective in the treatment of open fractures, although the underlying mechanism is not clear. This article aimed to investigate the effects of NPWT on muscle-derived stem cell (MDSC) osteoblastic differentiation and the related potential mechanism. The cell proliferation rate was substantially increased in NPWT-treated MDSCs in comparison with a static group for 3 days. There was no observable effect on the apoptosis of MDSC treated with NPWT compared with the control group for 3 days...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28944919/proprotein-convertase-subtilisin-kexin-type-6-promotes-in%C3%A2-vitro-proliferation-migration-and-inflammatory-cytokine-secretion-of-synovial-fibroblast%C3%A2-like-cells-from-rheumatoid-arthritis-via-nuclear%C3%A2-%C3%AE%C2%BAb-signal-transducer-and-activator-of-transcription-3-and
#4
Huiyu Jiang, Lin Wang, Feifei Wang, Jihong Pan
Our previous studies demonstrated that expression of proprotein convertase subtilisin/kexin type 6 (PCSK6) is greatly enhanced in rheumatoid arthritis fibroblast‑like synoviocytes (RASFs), and that PCSK6 inhibition decreases cell proliferation, migration and invasion. The present study aimed to investigate the functional role of PCSK6 in the hyperplasia of RASFs. Cultured RASFs from RA patients were stimulated with recombinant human (rh)PCSK6. Subsequent changes in proliferation, invasion, migration and the secretion of inflammatory cytokines were measured in vitro using MTT, wound healing and Transwell assays, and ELISA...
September 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944873/coxsackieviruses-b3-infection-of-myocardial-microvascular-endothelial-cells-activates-fractalkine-via-the-erk1-2-signaling-pathway
#5
Jili Wen, Congxin Huang
Infections by pathogens may lead to cardiovascular diseases, including acute/chronic myocarditis. (Coxsackieviruses B3) CVB3 is considered to be the most common causative agent in m‑yocarditis, which can lead to dilated cardiomyopathy. The present study aimed to investigate the mechanism of CVB3‑infected myocardial microvascular endothelial cells. The CVB3 infection was detected by 50% tissue culture infective dose (TCID50). The role of fractalkine (FKN) in the infection was detected using western blotting and RNA interference...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944867/succinate-promotes-skeletal-muscle-protein-synthesis-via-erk1-2-signaling-pathway
#6
Yexian Yuan, Yaqiong Xu, Jingren Xu, Bingqing Liang, Xingcai Cai, Canjun Zhu, Lina Wang, Songbo Wang, Xiaotong Zhu, Ping Gao, Xiuqi Wang, Yongliang Zhang, Qingyan Jiang, Gang Shu
It is well known that endurance training is effective to attenuate skeletal muscle atrophy. Succinate is a typical TCA metabolite, of which exercise could dramatically increase the content. The present study aimed to investigate the effect of succinate on protein synthesis in skeletal muscle, and try to delineate the underlying mechanism. The in vitro study revealed that succinate dose‑dependently increased protein synthesis in C2C12 myotube along with the enhancement of phosphorylation levels of AKT Serine/Threonine Kinase 1(Akt), mammalian target of rapamycin, S6, eukaryotic translation initiation factor 4E, 4E binding protein 1 and forkhead box O (FoxO) 3a...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944860/polyaniline-promotes-peripheral-nerve-regeneration-by-enhancement-of-the-brain%C3%A2-derived-neurotrophic-factor-and-ciliary-neurotrophic-factor-expression-and-activation-of-the-erk1-2-mapk-signaling-pathway
#7
Lin Fan, Yan Xiong, Zhen Fu, Dingfeng Xu, Lei Wang, Yong Chen, Haoyang Xia, Na Peng, Shaojun Ye, Yanfeng Wang, Lina Zhang, Qifa Ye
A previous study has demonstrated a progression in the nerve regeneration by polyaniline/cellulose (PANI/RC), although the underlying mechanism was not elucidated. In the present study, regenerated nerves were investigated, using histological techniques, functional assays and western blot analysis. The triceps surae muscle weight ratio percentages of the sham, regenerated cellulose (RC) and the PANI/RC groups were 38.88±4.76 and 76.32±7.11%, respectively. The thickness of the myelin sheath for the aforementioned groups were as follows: 1...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944626/caveolin-1-secreted-from-adipose-tissues-and-adipocytes-functions-as-an-adipogenesis-enhancer
#8
Chia-Chu Chang, Chen-Yu Chen, Hui-Chin Wen, Chih-Yang Huang, Ming-Shiu Hung, Hsi-Chi Lu, Woan-Ling Chen, Chung-Ho Chang
OBJECTIVE: Caveolin-1 (Cav-1) is expressed abundantly in adipose tissue and involved in many physiological processes. While Cav-1 has been reported to be secreted in pancreatic acinar cells and LNCaP prostate cancer cells, its secretion from adipose tissue awaits investigation. METHODS: Cav-1 secretion from 3T3-L1 adipocytes and fat tissues from normal chow diet- and high-fat diet (HFD)-fed mice was measured. Functions and uptake of secreted Cav-1 proteins were assessed by adding Cav-1 back to preadipocytes and LNCaP cells...
September 25, 2017: Obesity
https://www.readbyqxmd.com/read/28943444/p-p-methoxyl-diphenyl-diselenide-loaded-polymeric-nanocapsules-as-a-novel-approach-to-inflammatory-pain-treatment-behavioral-biochemistry-and-molecular-evidence
#9
Marcel Henrique Marcondes Sari, Vanessa Angonesi Zborowski, Luana Mota Ferreira, Natália Silva Jardim, Allanna Valentini Barbieri, Letícia Cruz, Cristina Wayne Nogueira
The current study investigated the effect of organoselenium compound p,p'-methoxyl-diphenyl diselenide [(OMePhSe)2], free or incorporated into nanocapsules, on behavioral, biochemical and molecular alterations in an inflammatory pain model induced by complete Freund's adjuvant (CFA). Male Swiss mice received an intraplantar injection of CFA in the hindpaw and 24 h later they were treated via the intragastric route with a single (OMePhSe)2 administration, in its free form (dissolved in canola oil) or (OMePhSe)2 NC...
September 21, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28943431/src-mediated-ligand-release-independent-egfr-transactivation-involves-tgf-%C3%AE-induced-smad3-activation-in-mesangial-cells
#10
Yan Chen, Fang-Fang Peng, Jing Jin, Hong-Min Chen, Hong Yu, Bai-Fang Zhang
A great deal of evidence highlighted the pathophysiologic importance of TGF-β1/Smad3 pathway in masangial extracellular matrix (ECM) accumulation, but some alternative signaling pathways are also involved. TGF-β was shown recently to induce rapid and transient epidermal-like growth factor receptor (EGFR) transactivation and subsequent fibronectin expression via heparin-binding epidermal-like growth factors (HB-EGF) release and binding in mesangial cells, which is independent of Smad2 activation. However, whether TGF-β could induce persistent EGFR transactivation remains to be identified...
September 21, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28943399/testosterone-bicalutamide-antagonism-at-the-predicted-extracellular-androgen-binding-site-of-zip9
#11
Ahmed Bulldan, Viveka Nand Malviya, Neha Upmanyu, Lutz Konrad, Georgios Scheiner-Bobis
ZIP9 is a Zn(2+) transporter, testosterone receptor, and mediator of signaling events through G-proteins. Despite these pivotal properties, however, its physiological and pathophysiological significance has not yet been comprehensively addressed. Using a cell line that lacks the classical androgen receptor we show that ZIP9-mediated phosphorylation of Erk1/2, CREB, or ATF-1 and expression of claudin-5 and zonula occludens-1 by testosterone can be completely antagonized by bicalutamide (Casodex), an anti-androgen of significant clinical impact...
September 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28943238/free-fatty-acid-receptor-4-ffa4-modulates-ros-generation-and-cox-2-expression-via-the-c-terminal-%C3%AE-arrestin-phosphosensor-in-raw-264-7-macrophages
#12
Ameneh Cheshmehkani, Ilya S Senatorov, Jyothi Dhuguru, Ola Ghoneim, Nader H Moniri
Agonism of the G protein-coupled Free-Fatty Acid receptor-4 (FFA4) has been shown to promote numerous anti-inflammatory effects in macrophages that arise due to interaction with β-arrestin partner proteins. Humans express functionally distinct short and long FFA4 splice variants, such that FFA4-S signals through Gαq/11 and β-arrestin, while FFA4-L is intrinsically biased solely towards β-arrestin signaling. Recently, we and others have shown that phosphorylation of the FFA4 C-terminal tail is responsible for β-arrestin interactability and signaling...
September 21, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28940899/low-intensity-pulsed-ultrasound-activates-erk1-2-and-pi3k-akt-signalling-pathways-and-promotes-the-proliferation-of-human-amnion-derived-mesenchymal-stem-cells
#13
Li Ling, Tianqin Wei, Lianli He, Yaping Wang, Yan Wang, Xiushan Feng, Wenqian Zhang, Zhengai Xiong
OBJECTIVES: This study was to investigate the effect and mechanism of low-intensity pulsed ultrasound (LIPUS) on the proliferation of human amnion-derived mesenchymal stem cells (hAD-MSCs). METHODS: Human amnion-derived mesenchymal stem cells were isolated from the amnion of term placentas and identified by flow cytometry and differentiation culture. Proliferation of hAD-MSCs was investigated by Cell Counting Kit-8, cell cycle and EdU assays. Western blotting was used to determine the protein expression levels...
September 22, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28939773/pleiotrophin-a-multifunctional-cytokine-and-growth-factor-induces-leukocyte-responses-through-the-integrin-mac-1
#14
Di Shen, Nataly P Podolnikova, Valentin P Yakubenko, Christopher L Ardell, Arnat Balabiyev, Tatiana P Ugarova, Xu Wang
Pleiotrophin (PTN) is a multifunctional, cationic, glycosaminoglycan-binding cytokine and growth factor involved in numerous physiological and pathological processes, including tissue repair and inflammation-related diseases. PTN has been shown to promote leukocyte responses by inducing their migration and expression of inflammatory cytokines. However, the mechanisms through which PTN mediates these responses remain unclear. Here, we identified the integrin Mac-1 (αMβ2, CD11b/CD18) as the receptor mediating macrophage adhesion and migration to PTN...
September 22, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28939558/targeting-the-mapk-signaling-pathway-in-cancer-promising-preclinical-activity-with-the-novel-selective-erk1-2-inhibitor-bvd-523-ulixertinib
#15
Ursula A Germann, Brinley F Furey, William Markland, Russell R Hoover, Alex M Aronov, Jeffrey J Roix, Micheal Hale, Diane M Boucher, David A Sorrell, Gabriel Martinez-Botella, Matthew Fitzgibbon, Paul Shapiro, Michael J Wick, Ramin Samadani, Kathryn Meshaw, Anna Groover, Gary DeCrescenzo, Mark Namchuk, Caroline M Emery, Saurabh Saha, Dean J Welsch
Aberrant activation of signaling through the RAS-RAF-MEK-ERK (MAPK) pathway is implicated in numerous cancers, making it an attractive therapeutic target. Although BRAF- and MEK-targeted combination therapy has demonstrated significant benefit beyond single-agent options, the majority of patients develop resistance and disease progression after approximately 12 months. Reactivation of ERK signaling is a common driver of resistance in this setting. Here we report the discovery of BVD-523 (ulixertinib), a novel, reversible, ATP-competitive ERK1/2 inhibitor with high potency and ERK1/2 selectivity...
September 22, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/28939254/inhibition-of-mek-erk1-2-map-kinase-signalling-pathway-reduces-rabies-virus-induced-pathologies-in-mouse-model
#16
Venkataravanappa Manjunatha, Karam Pal Singh, Mani Saminathan, Rajendra Singh, Nayakwadi Shivasharanappa, Channakeshava Sokke Umeshappa, Kuldeep Dhama, Gundallahalli Bayyappa Manjunathareddy
The extracellular signal-regulated kinase (ERK) pathway has been shown to regulate pathogenesis of many viral infections, but its role during rabies virus (RV) infection in vivo is not clear. In the present study, we investigated the potential role of MEK-ERK1/2 signalling pathway in the pathogenesis of rabies in mouse model and its regulatory effects on pro-inflammatory cytokines and other mediators of immunity, and kinetics of immune cells. Mice were infected with 25 LD50 of challenge virus standard (CVS) strain of RV by intracerebral (i...
September 19, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28939105/protein-kinase-c-eta-regulates-mcl-1-level-via-erk1
#17
Deepanwita Pal, Alakananda Basu
Protein kinase C (PKC)-eta (PKCη) is a member of the novel category of PKC family. It is overexpressed in breast cancer and was shown to inhibit apoptosis and contribute to chemoresistance. Since the anti-apoptotic Bcl-2 family protein myeloid cell leukemia-1 (Mcl-1) plays an important role in breast cancer cell survival and chemoresistance, we investigated if PKCη regulates Mcl-1 level. Silencing of PKCη decreased Mcl-1 in several breast cancer cells, including MCF-7 and T47D cells. PKCη depletion had no effect on MCL1 mRNA but the decrease in Mcl-1 by PKCη knockdown was blocked by proteasomal inhibitors, such as MG132 and lactacystin...
September 19, 2017: Cellular Signalling
https://www.readbyqxmd.com/read/28938613/high-phlpp1-expression-levels-predicts-longer-time-of-acquired-resistance-to-egfr-tyrosine-kinase-inhibitors-in-patients-with-lung-adenocarcinoma
#18
Youyou Xie, Dongqing Lv, Wei Wang, Minhua Ye, Xiaofeng Chen, Haihua Yang
BACKGROUND: In spite of an initial good response to epidermal growth factor receptor tyrosine kinase inhibitors (EGFR TKIs) in lung adenocarcinoma patients, resistance to treatment eventually occurs. Epidermal growth factor receptor (EGFR) activation stimulates Ras/Raf/Erk/MAPK and influences PI3K/Akt pathways, respectively. PHLPP negatively regulates PI3K/Akt and the RAF/RAS/ERK signaling pathways. Our study aimed to investigate the association between PH domain leucine-rich-repeats protein phosphatase (PHLPP) expression levels and the acquired resistance to EGFR TKIs in lung adenocarcinoma...
August 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/28938523/hypothermia-induced-by-anesthesia-regulates-various-signals-expressions-in-the-hippocampus-of-animals
#19
Huimei Hao, Shanshan Wang
Though important for a variety of medical procedures, general anesthesia is not a problem free. Some anesthetics have been suggested to affect a number of signals, which are associated with memory consolidation and cognition. In the present study, we attempted to investigate the molecular mechanism of anesthesia-regulated processes. We found that under hypothermic condition, anesthetic of isoflurane enhances various signals expression in hippocampus, including Hspd1, Actb, Mgst1, THBS4, Syp, C1QC, Serpine, Plat, and Ngf, which were related to cellular stress, neural plasticity responses, and hippocampal injury...
September 18, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28938449/tsh-igf-1-receptor-cross-talk-rapidly-activates-extracellular-signal-regulated-kinases-in-multiple-cell-types
#20
Christine C Krieger, Joseph D Perry, Sarah J Morgan, George J Kahaly, Marvin C Gershengorn
We previously showed that TSH/IGF-1 receptor cross-talk appears to be involved in Graves' orbitopathy (GO) pathogenesis and up-regulation of thyroid-specific genes in human thyrocytes. In orbital fibroblasts from GO patients (GOFs), co-administration of TSH and IGF-1 induces synergistic increases in hyaluronan secretion. In human thyrocytes, TSH plus IGF-1 synergistically increased expression of sodium-iodide symporter that appeared to involve ERK1/2 activation. However, the details of ERK1/2 activation were not known nor whether ERK1/2 was involved in this synergism in other cell types...
August 11, 2017: Endocrinology
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