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Red Nucleus

Ahmed M Elkady, Dana Cobzas, Hongfu Sun, Gregg Blevins, Alan H Wilman
BACKGROUND: Combined R2* and quantitative susceptibility (QS) has been previously used in cross-sectional multiple sclerosis (MS) studies to distinguish deep gray matter (DGM) iron accumulation and demyelination. PURPOSE: We propose and apply discriminative analysis of regional evolution (DARE) to define specific changes in MS and healthy DGM. STUDY TYPE: Longitudinal (baseline and 2-year follow-up) retrospective study. SUBJECTS: Twenty-seven relapsing-remitting MS (RRMS), 17 progressive MS (PMS), and corresponding age-matched healthy subjects...
March 14, 2018: Journal of Magnetic Resonance Imaging: JMRI
A Papadimitriou, I Ketikidis, M-E K Stathopoulou, C N Banti, C Papachristodoulou, L Zoumpoulakis, S Agathopoulos, G V Vagenas, S K Hadjikakou
Curcumin (Curc) reacts with zinc di‑iodine (ZnI2 ) in 2:1molar ratio in the presence of an excess of a base triethylamine ((CH3 CH2 )3 N) in methanol (CH3 OH) solution towards the amorphous solid material of formula [ZnI2 (Curc)2 ] (1). The complex was characterized by melting point (m.p.), Fourier Transform-Infra Red (FT-IR) and Nuclear Magnetic Resonance of hydrogen nucleus (1 H NMR) spectroscopy. The formula of 1 was determined by X-ray fluorescence (XRF) analysis. The retention of the structure in solution was confirmed by1 H NMR spectroscopy...
March 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
Christine Senneca, Gabriel Garcia, Justin F Rosenberg, Darryl Heard, Erin Porter, Lilian Olivera, Lisa Farina
A neutered male Siberian tiger ( Panthera tigris altaica) presented with paraplegia of 5 days' duration. Thoracolumbar magnetic resonance imaging (MRI) revealed a focal, linear T2-W hyperintense intramedullary lesion at the level of T10-11 as well as reduced size of the nucleus pulposus of the T10-11 intervertebral disc. The animal was humanely euthanatized as a result of the severity of clinical signs and the poor prognosis. Gross necropsy revealed a dark red, firm focus on the spinal cord at the level of the T11 nerve roots...
March 2018: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
Stefania Ferraro, Anna Nigri, Maria Grazia Bruzzone, Luca Brivio, Alberto Proietti Cecchini, Mattia Verri, Luisa Chiapparini, Massimo Leone
Objective We tested the hypothesis of a defective functional connectivity between the posterior hypothalamus and diencephalic-mesencephalic regions in chronic cluster headache based on: a) clinical and neuro-endocrinological findings in cluster headache patients; b) neuroimaging findings during cluster headache attacks; c) neuroimaging findings in drug-refractory chronic cluster headache patients improved after successful deep brain stimulation. Methods Resting state functional magnetic resonance imaging, associated with a seed-based approach, was employed to investigate the functional connectivity of the posterior hypothalamus in chronic cluster headache patients (n = 17) compared to age and sex-matched healthy subjects (n = 16)...
January 1, 2018: Cephalalgia: An International Journal of Headache
Chenwen Xiao, Ling Li, Limin Lao, Yan Liu, Qiang Wei, Quan'an Ji, Guochang Sun, Fucheng Lin, Jiaoyu Wang, Guolian Bao
Trichophyton mentagrophytes is a fungus that causes skin disease in humans and other animals worldwide. Studies on molecular biology and fluorescent labeling of the fungus are limited. Here, we applied mCherry for the first time in T. mentagrophytes to label the fungus and its organelles. We constructed four expression vectors of mCherry or mCherry fusions containing a variety of resistance markers and promoters, which were then integrated, together with two previous mCherry expression vectors, in T. mentagrophytes via Agrobacterium tumefaciens-mediated transformation (AtMT)...
March 1, 2018: FEMS Microbiology Letters
Shaoqing Zang, Xin Zhang, Jingcheng Zhang, Chen Li, Jingguang Wei, Qiwei Qin
The eukaryotic initiation factor 2 alpha subunit (eIF2α) is a key translation regulator that plays an important role in different cellular pressures and stimuli, including virus infection. In the present study, an eIF2α homolog (EceIF2α) from the orange-spotted grouper (Epinephelus coioides) was cloned and its roles during fish viral infection were characterized. EceIF2α encodes a putative protein of 315 amino acid residues, and shares a high degree of similarity with eIF2αs from other species. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that EceIF2α was distributed in all examined tissues...
February 15, 2018: Fish & Shellfish Immunology
Qi Wu, Jing Li, Pingping Song, Jing Chen, Yuanzhi Xu, Shengcai Qi, Ji Ma, Qiuhui Pan
PURPOSE: Neurotrophin receptor-interacting MAGE homologue (Nrage) plays an important role in bone development and the metabolism of normal skeletal structures. Our previous study showed that Nrage inhibited the odontogenic differentiation of mouse dental pulp cells. However, the potential roles and mechanism of Nrage in regulating odontogenic differentiation are unknown. The aim of this study was to investigate the molecular mechanism of Nrage in odontogenic differentiation of mouse odontoblast-like cells...
February 15, 2018: Connective Tissue Research
Kang Li, Yan Li, Jie Mi, Lu Mao, Xiuguo Han, Jie Zhao
Oxidative stress induced disc cell apoptosis plays an important role in intervertebral disc (IVD) degeneration. The present study aims to investigate effects of resveratrol (RV), a natural polyphenol compound, on sodium nitroprusside (SNP) induced nucleus pulposus (NP) cell apoptosis and related mechanism. Rat NP cells were pretreated with RV, N-acetyl cysteine (NAC) and carboxy-PTIO (PTIO) before SNP treatment. Cell Counting Kit-8 assay was carried out for cell viability evaluation. Annexin V/propidium iodide (PI), Hoechst 33258 and Actin‑Tracker Green and Tubulin-Tracker Red staining were conducted to detect NP cell apoptosis and apoptotic structural changes...
February 6, 2018: International Journal of Molecular Medicine
A S Rix, T J Grove, K M O'Brien
The long evolution of the Antarctic perciform suborder of Notothenioidei in the icy, oxygen-rich waters of the Southern Ocean may have reduced selective pressure to maintain a hypoxic response. To test this hypothesis, cDNA of the key transcriptional regulator of hypoxic genes, hypoxia-inducible factor-1α (HIF-1α), was sequenced in heart ventricles of the red-blooded notothenioid, Notothenia coriiceps, and the hemoglobinless icefish, Chaenocephalus aceratus. HIF-1α cDNA is 4500 base pairs (bp) long and encodes 755 amino acids in N...
December 2017: Polar Biology
Astrid J F Thielen, Sacha Zeerleder, Diana Wouters
The complement system represents the first line of defense that is involved in the clearance of pathogens, dying cells and immune complexes via opsonization, induction of an inflammatory response and the formation of a lytic pore. Red blood cells (RBCs) are very important for the delivery of oxygen to tissues and are continuously in contact with complement proteins in the blood plasma. To prevent complement activation on RBCs, various complement regulatory proteins can be found in plasma and on the cell membrane...
January 31, 2018: Blood Reviews
Bence György, Lilian Cruz, David Yellen, Massimo Aufiero, Isabel Alland, Xuan Zhang, Maria Ericsson, Cornel Fraefel, Yu-Ching Li, Shuko Takeda, Bradley T Hyman, Xandra O Breakefield
Most cases of early onset torsion dystonia (DYT1) are caused by a 3-base pair deletion in one allele of the TOR1A gene causing loss of a glutamate in torsinA, a luminal protein in the nuclear envelope. This dominantly inherited neurologic disease has reduced penetrance and no other medical manifestations. It has been challenging to understand the neuronal abnormalities as cells and mouse models which are heterozygous (Het) for the mutant allele are quite similar to wild-type (WT) controls. Here we found that patient fibroblasts and mouse neurons Het for this mutation showed significant differences from WT cells in several parameters revealed by infection with herpes simplex virus type 1 (HSV) which replicates in the nucleus and egresses out through the nuclear envelope...
February 2, 2018: Scientific Reports
Jessica C Tempany, Jie Hs Zhou, Philip D Hodgkin, Vanessa L Bryant
The discovery of cell division tracking properties of 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE) by Lyons and Parish in 1994 led to a broad range of new methods and numerous important biological discoveries. After labeling, CFSE is attached to free amine groups and intracellular proteins in the cytoplasm and nucleus of a cell, and halves in fluorescence intensity with each round of cell division, enabling enumeration of the number of divisions a cell has undergone. A range of popular division tracking dyes were subsequently developed, including CellTrace Violet (CTV), making available the green fluorescent channel previously occupied by CFSE...
October 19, 2017: Immunology and Cell Biology
Sharon J Brown, Sarah A Turner, Birender S Balain, Neil T Davidson, Sally Roberts
Objective The purpose of this study was to investigate whether a simple, biologically robust method for inducing calcification of degenerate intervertebral discs (IVD) could be developed to provide an alternative treatment for patients requiring spinal fusion. Design Nucleus pulposus (NP) cells isolated from 14 human IVDs were cultured in monolayer and exposed to osteogenic medium, 1,25-dihydroxyvitamin D3 (VitD3), parathyroid hormone (PTH), and bone morphogenic proteins (BMPs) 2/7 to determine if they could become osteogenic...
January 1, 2018: Cartilage
Duo Li, Zhenzhen Qiao, Yanru Yu, Jinlu Tang, Xiaoxiao He, Hui Shi, Xiaosheng Ye, Yanli Lei, Kemin Wang
A facile, general and turn-on nucleus imaging strategy was first developed based on in situ fluorescence activation of C-rich dark silver nanoclusters by G-rich telomeres. After a simple incubation without washing, nanoclusters could selectively stain the nucleus with intense red luminescence, which was confirmed using fixed/living cells and several cell lines.
January 12, 2018: Chemical Communications: Chem Comm
David Perez-Guaita, Kamila Kochan, Mitchell Batty, Christian Doerig, Jose Garcia-Bustos, Shirley Josefina Espinoza Herrera, Don McNaughton, Philip Heraud, Bayden R Wood
Atomic Force Microscopy-Infrared (AFM-IR) spectroscopy is a powerful new technique that can be applied to study molecular composition of cells and tissues at the nano-scale. AFM-IR maps are acquired using single wavenumber value: they show either the absorbance plotted against a single wavenumber value, or a ratio of two absorbance values. Here we implement multivariate image analysis to generate multivariate AFM-IR maps, and use this approach to resolve subcellular structural information in red blood cells infected with Plasmodium falciparum at different stages of development...
January 12, 2018: Analytical Chemistry
Marina Mann, Somayeh Fattah-Hosseini, El-Desouky Ammar, Richard Stange, EricaRose Warrick, Kasie Sturgeon, Robert Shatters, Michelle Heck
"Candidatus Liberibacter asiaticus" is the causative bacterium associated with citrus greening disease. "Ca. L. asiaticus" is transmitted by Diaphorina citri more efficiently when it is acquired by nymphs rather than adults. Why this occurs is not known. We compared midguts of D. citri reared on healthy or "Ca. L. asiaticus"-infected citrus trees using quantitative PCR, confocal microscopy, and mitochondrial superoxide staining for evidence of oxidative stress. Consistent with its classification as propagative, "Ca...
January 8, 2018: Infection and Immunity
Qifang Yang, Lina Zhou, Chen Liu, Daihong Liu, Yang Zhang, Chang Li, Yongning Shang, Xin Wei, Chuanming Li, Jian Wang
This study was performed to investigate iron deposition in the brain of type 2 diabetes mellitus (T2DM) patients using quantitative susceptibility mapping (QSM) and the associated cognitive impairments. Sixty patients diagnosed with T2DM were subjected to neuropsychological tests to determine their cognitive status, and the results were used to subdivide the patients into a T2DM without mild cognitive impairment (MCI) group (n = 30) and a T2DM with MCI group (n = 30). All patients underwent high-resolution susceptibility-weighted imaging, and data processing was performed using SMART (Susceptibility Mapping and Phase Artifacts Removal Toolbox) software...
January 3, 2018: Brain Imaging and Behavior
Bing-Zhu Hou, Cheng Xu, Yuan-Yue Shen
Strawberry (Fragaria × ananassa) is a model plant for studying non-climacteric fruit ripening regulated by abscisic acid (ABA), however, its defined molecular mechanisms are yet not fully understood. Here, a strawberry predicted leu-rich repeat (LRR) receptor-like kinase, red-initial protein kinase 1 (FaRIPK1), was screened and shown to interact with a putative ABA receptor, FaABAR, using a yeast two-hybrid assay. This association was confirmed by bimolecular fluorescence complementation and co-immunoprecipitation assays, and shown to occur in the nucleus...
December 21, 2017: Journal of Experimental Botany
Upashi Goswami, Anushree Dutta, Asif Raza, Raghuram Kandimalla, Sanjeeb Kalita, Siddhartha Sankar Ghosh, Arun Chattopadhyay
Transferrin (Tf) templated luminescent blue copper nanoclusters (Tf-CuNCs) are synthesized. They are further formulated into spherical transferrin copper nanocluster-doxorubicin nanoparticles (Tf-CuNC-Dox-NPs) based on electrostatic interaction with doxorubicin (Dox). The as synthesized Tf-CuNC-Dox-NPs are explored for bioimaging and targeted drug delivery to delineate high therapeutic efficacy. Förster resonance energy transfer (FRET) within the Tf-CuNC-Dox-NPs exhibited striking red luminescence, wherein the blue luminescence of Tf-CuNCs (donor) is quenched due to absorption by Dox (acceptor)...
December 26, 2017: ACS Applied Materials & Interfaces
Ji-Hye Song, Hee-Bum Kang, Seung-Ho Park, Ji-Hoon Jeong, Jeongjin Park, Yanghee You, Yoo-Hyun Lee, Jeongmin Lee, Eungpil Kim, Kyung-Chul Choi, Woojin Jun
Porphyra tenera, also known as nori, is a red algal species of seaweed. It is cultivated in Asia for culinary purposes. We report that P. tenera extract (PTE) enhances the immune response in mouse macrophages. We found that P. tenera extract regulates the NF-κB IκB kinase (IKK) signaling pathway, and we assessed the expression and translocation of p65, a subunit of NF-κB, in RAW264.7 mouse macrophage cells after treatment with PTE. We also investigated the effects of 10% ethanol PTE (PTE10) in RAW264.7 cells...
December 2017: Journal of Medicinal Food
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