keyword
MENU ▼
Read by QxMD icon Read
search

SH3

keyword
https://www.readbyqxmd.com/read/29445232/the-cerebellum-of-patients-with-steatohepatitis-shows-lymphocyte-infiltration-microglial-activation-and-loss-of-purkinje-and-granular-neurons
#1
Tiziano Balzano, Jerónimo Forteza, Pilar Molina, Juan Giner, Ana Monzó, Jennifer Sancho-Jiménez, Amparo Urios, Carmina Montoliu, Vicente Felipo
Peripheral inflammation contributes to minimal hepatic encephalopathy in chronic liver diseases, which could be mediated by neuroinflammation. Neuroinflammation in cerebellum of patients with chronic liver diseases has not been studied in detail. Our aim was to analyze in cerebellum of patients with different grades of liver disease, from mild steatohepatitis to cirrhosis and hepatic encephalopathy: (a) neuronal density in Purkinje and granular layers; (b) microglial activation; (c) astrocyte activation; (d) peripheral lymphocytes infiltration; (e) subtypes of lymphocytes infiltrated...
February 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29426960/two-microcephaly-associated-novel-missense-mutations-in-cask-specifically-disrupt-the-cask-neurexin-interaction
#2
Leslie E W LaConte, Vrushali Chavan, Abdallah F Elias, Cynthia Hudson, Corbin Schwanke, Katie Styren, Jonathan Shoof, Fernando Kok, Sarika Srivastava, Konark Mukherjee
Deletion and truncation mutations in the X-linked gene CASK are associated with severe intellectual disability (ID), microcephaly and pontine and cerebellar hypoplasia in girls (MICPCH). The molecular origin of CASK-linked MICPCH is presumed to be due to disruption of the CASK-Tbr-1 interaction. This hypothesis, however, has not been directly tested. Missense variants in CASK are typically asymptomatic in girls. We report three severely affected girls with heterozygous CASK missense mutations (M519T (2), G659D (1)) who exhibit ID, microcephaly, and hindbrain hypoplasia...
February 9, 2018: Human Genetics
https://www.readbyqxmd.com/read/29416010/role-of-c-abl-and-nephrin-in-podocyte-cytoskeletal-remodeling-induced-by-angiotensin-ii
#3
Yiqiong Ma, Qian Yang, Zhentong Zhong, Wei Liang, Lu Zhang, Yingjie Yang, Guohua Ding
Our previous study showed that angiotensin II (Ang II) exposure diminished the interaction between nephrin and c-Abl, then c-Abl mediated SHIP2-Akt pathway in the process of podocyte injury in vivo and vitro. However, the relationship between nephrin and c-Abl was unknown. Recently, various studies showed that nephrin was required for cytoskeletal remodeling in glomerular podocytes. But its specific mechanisms remain incompletely understood. As a nonreceptor tyrosine kinase involved in cytoskeletal regulation, c-Abl may be a candidate of signaling proteins interacting with Src homology 2/3 (SH2/SH3) domains of nephrin...
February 7, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29405497/structural-insights-into-the-binding-and-catalytic-mechanisms-of-the-listeria-monocytogenes-bacteriophage-glycosyl-hydrolase-plyp40
#4
Patricia Romero, Sergio G Bartual, Mathias Schmelcher, Chaim Glück, Juan A Hermoso, Martin J Loessner
Endolysins are bacteriophage-encoded peptidoglycan hydrolases that specifically degrade the bacterial cell wall at the end of the phage lytic cycle. They feature a distinct modular architecture, consisting of enzymatically active domains (EADs) and cell wall binding domains (CBDs). Structural analysis of the complete enzymes or individual domains is required for better understanding the mechanisms of peptidoglycan degradation and provides guidelines for the rational design of chimeric enzymes. We here report the crystal structure of the EAD of PlyP40, a member of the GH-25 family of glycosyl hydrolases, and the first muramidase reported for Listeria phages...
February 5, 2018: Molecular Microbiology
https://www.readbyqxmd.com/read/29386119/ip3-receptor-dependent-cytoplasmic-ca2-signals-are-tightly-controlled-by-cav%C3%AE-3
#5
Anouar Belkacemi, Xin Hui, Barbara Wardas, Matthias W Laschke, Ulrich Wissenbach, Michael D Menger, Peter Lipp, Andreas Beck, Veit Flockerzi
Voltage-gated calcium channels (Cavs) are major Ca2+ entry pathways in excitable cells. Their β subunits facilitate membrane trafficking of the channel's ion-conducting α1 pore and modulate its gating properties. We report that one β subunit, β3, reduces Ca2+ release following stimulation of phospholipase C-coupled receptors and inositol 1,4,5-trisphosphate (IP3) formation. This effect requires the SH3-HOOK domain of Cavβ3, includes physical β3/IP3 receptor interaction, and prevails when agonist-induced IP3 formation is bypassed by photolysis of caged IP3...
January 30, 2018: Cell Reports
https://www.readbyqxmd.com/read/29363593/stac-proteins-associate-to-the-iq-domain-of-cav1-2-and-inhibit-calcium-dependent-inactivation
#6
Marta Campiglio, Pierre Costé de Bagneaux, Nadine J Ortner, Petronel Tuluc, Filip Van Petegem, Bernhard E Flucher
The adaptor proteins STAC1, STAC2, and STAC3 represent a newly identified family of regulators of voltage-gated calcium channel (CaV) trafficking and function. The skeletal muscle isoform STAC3 is essential for excitation-contraction coupling and its mutation causes severe muscle disease. Recently, two distinct molecular domains in STAC3 were identified, necessary for its functional interaction with CaV1.1: the C1 domain, which recruits STAC proteins to the calcium channel complex in skeletal muscle triads, and the SH3-1 domain, involved in excitation-contraction coupling...
January 23, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29358330/histone-variant-h3-3-mediated-chromatin-remodeling-is-essential-for-paternal-genome-activation-in-mouse-preimplantation-embryos
#7
Qingran Kong, Laura A Banaszynski, Fuqiang Geng, Xiaolei Zhang, Jiaming Zhang, Heng Zhang, Claire Liane O'Neill, Peidong Yan, Zhonghua Liu, Koji Shido, Gianpiero D Palermo, C David Allis, Shahin Rafii, Zev Rosenwaks, Duancheng Wen
Derepression of chromatin-mediated transcriptional repression of paternal and maternal genomes is considered the first major step that initiates zygotic gene expression after fertilization. The histone variant H3.3 is present in both male and female gametes and is thought to be important for remodeling the paternal and maternal genomes for activation during both fertilization and embryogenesis. However, the underlying mechanisms remain poorly understood. Using our H3.3B-HA-tagged mouse model, engineered to report H3...
January 22, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29330290/dual-inhibition-of-cdk4-and-cdk2-via-targeting-p27-tyrosine-phosphorylation-induces-a-potent-and-durable-response-in-breast-cancer-cells
#8
Priyank Patel, Vladislav Tsiperson, Susan R S Gottesman, Jonathan Somma, Stacy W Blain
Cyclin-dependent kinase 4/6 (CDK4/6)-specific inhibitors, such as palbociclib, have shown clinical efficacy, but primary or secondary resistance has emerged as a problem. To develop more effective therapeutic approaches, investigation is needed into the mechanisms of resistance or adaption. Here, it is demonstrated that CDK2 compensates for loss of CDK4 activity to rescue palbociclib-arrested breast cancer cells, suggesting that inhibition of both kinases is required to achieve durable response. In addition, a novel strategy is described to inhibit tyrosine phosphorylation of p27Kip1 (CDKN1B) and simultaneously inhibit both CDK2 and CDK4...
January 12, 2018: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/29328992/transcriptome-wide-identification-and-competitive-disruption-of-sacum-binding-partners-in-human-colorectal-cancer
#9
Yinguang Zhang, Yongwang Zhang, Yuxiang Zhang
Human sacum is regulatory adaptor protein involved in cellular signaling network of colorectal cancer. Molecular evidences suggest that the protein is integrated into oncogenic signaling network by binding to SH3-containing proteins through its proline-rich motifs. In this study, we have performed a transcriptome-wide analysis and identification of sacum-binding partners in the genome profile of human colorectal cancer. The sacum-binding potency of SH3-containing proteins found in colorectal cancer was investigated by using bioinformatics modeling and intermolecular binding analysis...
December 30, 2017: Journal of Molecular Graphics & Modelling
https://www.readbyqxmd.com/read/29321253/role-of-the-hof1-cyk3-interaction-in-cleavage-furrow-ingression-and-primary-septum-formation-during-yeast-cytokinesis
#10
Meng Wang, Ryuichi Nishihama, Masayuki Onishi, John R Pringle
In Saccharomyces cerevisiae, it is well established that Hof1, Cyk3, and Inn1 contribute to septum formation and cytokinesis. Because hof1∆ and cyk3∆ single mutants have relatively mild defects but hof1∆ cyk3∆ double mutants are nearly dead, it has been hypothesized that these proteins contribute to parallel pathways. However, there is also evidence that they interact physically. In this study, we examined this interaction and its functional significance in detail. Our data indicate that the interaction (i) is mediated by a direct binding of the Hof1 SH3 domain to a proline-rich motif in Cyk3; (ii) occurs specifically at the time of cytokinesis but is independent of the (hyper)phosphorylation of both proteins that occurs at about the same time; (iii) is dispensable for the normal localization of both proteins; (iv) is essential for normal primary-septum formation and a normal rate of cleavage-furrow ingression; and (v) becomes critical for growth when either Inn1 or the type II myosin Myo1 (a key component of the contractile actomyosin ring) is absent...
January 10, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29320755/downregulated-sash1-expression-indicates-poor-clinical-prognosis-in-gastric-cancer
#11
Nan Zhou, Can Liu, Xudong Wang, Qinsheng Mao, Qin Jin, Peng Li
SASH1 (SAM- and SH3-domain containing 1), a novel candidate tumor suppressor, has attracted attention due to its role in intracellular signal transduction and its tumor prognostic value in diverse cancers. Reports have demonstrated that reduced SASH1 expression correlates with tumor proliferation, invasion, and metastasis. However, the expression and prognostic significance of SASH1 in gastric cancer (GC) remain unclear. In this study, 8 paired fresh-frozen GC tissues and corresponding gastric mucosal tissues were examined by Western blot to analyze the protein expression of SASH1...
January 7, 2018: Human Pathology
https://www.readbyqxmd.com/read/29319304/atp-site-ligands-determine-the-assembly-state-of-the-abelson-kinase-regulatory-core-via-the-activation-loop-conformation
#12
Rajesh Sonti, Ines Hertel-Hering, Allan Joaquim Lamontanara, Oliver Hantschel, Stephan Grzesiek
The constituent SH3, SH2, and kinase domains of the Abl kinase regulatory core can adopt an assembled (inactive) or a disassembled (active) conformation. We show that this assembly state strictly correlates with the conformation of the kinase activation loop induced by a total of 14 ATP site ligands, comprising all FDA-approved Bcr-Abl inhibiting drugs. The disassembly of the core by certain ligands can be explained by an induced push on the kinase N-lobe via A- and P-loop towards the SH3 domain. A similar sized P-loop motion is expected during nucleotide binding and release, which would be impeded in the assembled state, in agreement with its strongly reduced kinase activity...
January 10, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29314743/sensor-kinase-kinb-and-its-pathway-associated-key-factors-sense-the-signal-of-nutrition-starvation-in-sporulation-of-bacillus%C3%A2-subtilis
#13
Weipeng Liu, Zeying He, Feng Gao, Jinyuan Yan, Xiaowei Huang
Bacillus subtilis responds to environmental stress cues and develops endospores for survival. In the process of endospore formation, sporulation initiation is a vital stage and this stage is governed by autophosphorylation of the sensor histidine kinases. The second major sensor kinase KinB perceives the intracellular changes of GTP and ATP during sporulation. However, determination of the environmental signals as well as its related signaling pathway of KinB requires further elucidation. Our current study found that, contrary to the sporulation failure induced by ΔkinA in the nutrient-rich 2× SG medium, the sensor kinase KinB sensed the environmental cues in the nutrient-poor MM medium...
January 3, 2018: MicrobiologyOpen
https://www.readbyqxmd.com/read/29311151/tks-adaptor-proteins-at-a-glance
#14
REVIEW
Priyanka Saini, Sara A Courtneidge
Tyrosine kinase substrate (Tks) adaptor proteins are considered important regulators of various physiological and/or pathological processes, particularly cell migration and invasion, and cancer progression. These proteins contain PX and SH3 domains, and act as scaffolds, bringing membrane and cellular components in close proximity in structures known as invadopodia or podosomes. Tks proteins, analogous to the related proteins p47phox, p40phox and NoxO1, also facilitate local generation of reactive oxygen species (ROS), which aid in signaling at invadopodia and/or podosomes to promote their activity...
January 8, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/29286349/compressional-puffing-pretreatment-enhances-neuroprotective-effects-of-fucoidans-from-the-brown-seaweed-sargassum-hemiphyllum-on-6-hydroxydopamine-induced-apoptosis-in-sh-sy5y-cells
#15
Chun-Yung Huang, Chia-Hung Kuo, Po-Wei Chen
In this study, a compressional-puffing process (CPP) was used to pretreat Sargassum hemiphyllum (SH) and then fucoidan was extracted from SH by hot water. Three fucoidan extracts, namely SH1 (puffing at 0 kg/cm²); SH2 (puffing at 1.7 kg/cm²); and SH3 (puffing at 10.0 kg/cm²) were obtained, and their compositions and biological activities were evaluated. The results indicate that CPP increased the extraction yield, total sugar content, and molar ratios of sulfate/fucose of fucoidan and decreased molecular weight and impurities of fucoidan...
December 29, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29258818/remodeling-of-hiv-1-nef-structure-by-src-family-kinase-binding
#16
Jamie A Moroco, John Jeff Alvarado, Ryan P Staudt, Haibin Shic, Thomas E Wales, Thomas E Smithgall, John R Engen
The HIV-1 accessory protein Nef controls multiple aspects of the viral life cycle and host immune response, making it an attractive therapeutic target. Previous X-ray crystal structures of Nef in complex with key host cell binding partners have shed light on protein-protein interactions critical to Nef function. Crystal structures of Nef in complex with either the SH3 or tandem SH3-SH2 domains of Src-family kinases reveal distinct dimer conformations of Nef. However, the existence of these Nef dimer complexes in solution has not been established...
December 16, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/29241010/mechanisms-of-arsenic-sequestration-by-prosopis-juliflora-during-phytostabilization-of-metalliferous-mine-tailings
#17
Corin M Hammond, Robert Aubrey Root, Raina M Maier, Jon Chorover
Phytostabilization is a cost-effective long-term bioremediation technique for immobilization of metalliferous mine tailings. However the biogeochemical processes affecting metal(loid) molecular stabilization and mobility in the root zone remain poorly resolved. Roots of Prosopis juliflora grown for up to 36 months in compost-amended pyritic mine tailings from a federal Superfund site were investigated by micro-scale and bulk synchrotron X-ray absorption spectroscopy (XAS) and multiple energy micro X-ray fluorescence (ME-μXRF) imaging to determine iron, arsenic, sulfur speciation, abundance, and spatial distribution...
December 14, 2017: Environmental Science & Technology
https://www.readbyqxmd.com/read/29226323/cops5-and-lasp1-synergistically-interact-to-down-regulate-14-3-3%C3%AF-expression-and-promote-colorectal-cancer-progression-via-activating-pi3k-akt-pathway
#18
Rui Zhou, Ziyun Shao, Jian Liu, Wanqi Zhan, Qingzu Gao, Zhihua Pan, Ling Wu, Lijun Xu, Yanqing Ding, Liang Zhao
Overexpression of LIM and SH3 protein 1 (LASP1) is required for colorectal cancer (CRC) development and progression. Here, C-Jun activation domain-binding protein-1 (Jab1), also known as COPS5, was verified as a new LASP1-interacting protein through yeast two-hybrid assay. The role of COPS5 in LASP1-mediated CRC progression remains unknown. GST pull-down assay indicated that the SH3 domain of LASP1 could directly bind to MPN domain of COPS5. In vitro gain- and loss-of-function analyses revealed the stimulatory role of COPS5 on CRC cell proliferation, migration and invasion...
December 11, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29224116/chemical-shift-assignments-of-the-partially-deuterated-fyn-sh2-sh3-domain
#19
Fabien Kieken, Karine Loth, Nico van Nuland, Peter Tompa, Tom Lenaerts
Src Homology 2 and 3 (SH2 and SH3) are two key protein interaction modules involved in regulating the activity of many proteins such as tyrosine kinases and phosphatases by respective recognition of phosphotyrosine and proline-rich regions. In the Src family kinases, the inactive state of the protein is the direct result of the interaction of the SH2 and the SH3 domain with intra-molecular regions, leading to a closed structure incompetent with substrate modification. Here, we report the 1H, 15N and 13C backbone- and side-chain chemical shift assignments of the partially deuterated Fyn SH3-SH2 domain and structural differences between tandem and single domains...
December 9, 2017: Biomolecular NMR Assignments
https://www.readbyqxmd.com/read/29218600/the-sh3-binding-domain-of-cx43-participates-in-loop-tail-interactions-critical-for-cx43-hemichannel-activity
#20
Jegan Iyyathurai, Nan Wang, Catheleyne D'hondt, Jean X Jiang, Luc Leybaert, Geert Bultynck
Connexin 43 (Cx43) hemichannels establish local signaling networks via the release of ATP and other molecules, but their excessive opening may result in cell death. Hence, the activity of Cx43-hemichannels ought to be critically controlled. This involves interactions between the C-terminal tail (CT) and the cytoplasmic loop (CL), more particularly the L2 domain within CL. Previous work revealed an important role for the last nine amino acids of the Cx43 CT by targeting the L2 domain, as these nine amino acids were sufficient to restore the activity of CT-truncated Cx43-hemichannels...
December 7, 2017: Cellular and Molecular Life Sciences: CMLS
keyword
keyword
49154
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"