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Michael P Whyte, Emilina Lim, William H McAlister, Gary S Gottesman, Lien Trinh, Deborah J Veis, Vinieth N Bijanki, Matthew G Boden, Angela Nenninger, Steven Mumm, David Buchbinder
Pediatric granulomatous arthritis (PGA) refers to two formerly separate entities; autosomal dominant Blau syndrome (BS) and its sporadic phenocopy early-onset sarcoidosis (EOS). In 2001 BS and in 2005 EOS became explained by heterozygous mutations within the gene that encodes nucleotide-binding oligomerization domain-containing protein 2 (NOD2), also called caspase recruitment domain-containing protein 15 (CARD15). NOD2 is a microbe sensor in leukocyte cytosol that activates and regulates inflammation. PGA is characterized by a triad of auto-inflammatory problems (dermatitis, uveitis, and arthritis) in early childhood, which suggests the causal NOD2/CARD15 mutations are activating defects...
June 22, 2018: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
Shiwei Xie, Weiping Fang, Dan Wei, Yongjian Liu, Peng Yin, Jin Niu, Lixia Tian
A 25-days experiment was conducted to evaluate the effect of dietary Haematococcus pluvialis on growth, survival, immune response and stress tolerance ability of post-larval Litopenaeus vannamei. Post-larval white shrimp (mean initial weight 2.1 mg) were fed five isoenergic and isonitrogenous diets containing grade levels of Haematococcus pluvialis (0, 1.7, 3.3, 6.7 and 13.3 g kg-1 diet, respectively). Results indicated that 3.3 g Haematococcus pluvialis kg-1 diet increased the survival rate of post-larval white shrimp...
June 19, 2018: Fish & Shellfish Immunology
Fangfang Zhou, Yangjun Liu, Binbin Yang, Zhiping Hu
The sulfonylurea receptor 1 (SUR1)-regulated NCca-ATP channels were progressively upregulated and demonstrated unchecked opening in central nervous system (CNS) injury, which induced cerebral damage. Glibenclamide (GLI) can block NCca-ATP channels and consequently exert protective effects. Recent studies have found that GLI has antioxidative effects. In this study, we primarily explored the antioxidative effects of GLI in a rat model of intracerebral hemorrhage (ICH). We found that GLI could scavenge free radicals, reduce activated-caspase-3 expression, increase the Bcl-2/Bax ratio, inhibit apoptosis, and improve functional neurological outcomes in a rat model of ICH...
June 19, 2018: Brain Research Bulletin
Behnaz Ghaemi, Elnaz Shaabani, Roqya Najafi Taher, Saeedeh Jafari Nodooshan, Amin Sadeghpour, Sharmin Kharrazi, Amir Amani
In this study, we investigated whether ZnO coating on Ag nanoparticles tunes electron flux and hole figuration at Metal-semiconductor interface under UV radiation. This effect triggers photo activity and generation of reactive oxygen species (ROS) from Ag@ZnO nanoparticles which results in enhanced cytotoxic effects and apoptotic cell death in human breast cancer cells (MDA-MB231). In this context, up-regulation of apoptotic cascade proteins (i.e. Bax/Bcl2 association, p53, Cytochrome C and caspase-3) along with activation of oxidative stress proteins suggested occurrence of apoptosis by Ag@ZnO NPs in cancer cells through mitochondrial pathway...
June 22, 2018: ACS Applied Materials & Interfaces
Jonghae Lee, Yang Lee, Emily C LaVoy, Michihisa Umetani, Junyoung Hong, Yoonjung Park
Activation of the nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome mediates the release of pro-inflammatory cytokine interleukin (IL)-1β and thereby plays a pivotal role in the inflammatory response in vascular pathology. An active lifestyle has beneficial effects on inflammation-associated vascular dysfunction in obesity. However, it remains unclear how physical activity regulates NLRP3 inflammasome-mediated vascular dysfunction in obesity. Therefore, we explored the protective effect of physical activity on NLRP3 inflammasome-associated vascular dysfunction in mouse hearts, and the potential underlying mechanisms...
June 2018: Physiological Reports
Xiao-Ling Gao, Jiao-Jiao Yang, Shu-Juan Wang, Yan Chen, Bei Wang, Er-Jing Cheng, Jian-Nan Gong, Yan-Ting Dong, Dai Liu, Xiang-Li Wang, Ya-Qiong Huang, Dong-Dong An
Breast cancer is known as the most prevalent cancer in women worldwide, and has an undeniable negative impact on public health, both physically, and mentally. This study aims to investigate the effects of toll-like receptor 4 (TLR4) gene silencing on proliferation and apoptosis of human breast cancer cells to explore for a new theoretical basis for its treatment. TLR4 small interference RNA (siRNA) fragment recombinant plasmids were constructed, including TLR4 siRNA-1, TLR4 siRNA-2, and TLR4 siRNA-3. Human breast cancer MCF-7 and MDA-MB-231 cells were assigned into blank, negative control (NC), TLR4 siRNA-1, TLR4 siRNA-2, and TLR4 siRNA-3 groups...
June 22, 2018: Journal of Cellular Physiology
Afaf El-Ansary, Geir Bjørklund, Asma M Khemakhem, Laila Al-Ayadhi, Salvatore Chirumbolo, Abir Ben Bacha
Autism spectrum disorder (ASD) is a neuro-behavioral syndrome with a broad spectrum of different mechanisms and etiologies that are caused by abnormal brain development. To date, no highly reliable and effective diagnostic biomarker to assess ASD is available so far. The present study investigated the predictivity potential of some suggested markers in ASD diagnosis focusing onto the relative ratios of several plasma biomarkers of electron transport chain function, and mitochondrial metabolism in 41 patients with ASD evaluated for behavior deficits measured using Childhood Autism Rating Scales (CARS)...
June 22, 2018: Journal of Molecular Neuroscience: MN
Thao V Nguyen, Andrea C Alfaro, Fabrice Merien, Ronald Lulijwa, Tim Young
Copper is a common contaminant in aquatic environments, which may cause physiological dysfunction in marine organisms. However, the toxicity mechanisms of copper in marine bivalves is not fully understood. In this study, we applied an integrated approach that combines flow cytometry and Gas Chromatography-Mass Spectrometry (GC-MS)-based metabolomics to characterize cellular and molecular mechanisms of copper immunotoxicity in New Zealand Greenshell™ mussel (Perna canaliculus) haemolymph. Flow cytometric results showed significant increases in haemocyte mortality, production of reactive oxygen species and apoptosis (via alteration of caspase 3/7 and mitochondrial membrane potential) of haemocytes exposed to increasing total concentrations of Cu2+ (62...
June 22, 2018: Metallomics: Integrated Biometal Science
Kevin A Bockerstett, Luciana H Osaki, Christine P Petersen, Catherine W Cai, Chun Fung Wong, Thanh-Long M Nguyen, Eric L Ford, Daniel F Hoft, Jason C Mills, James R Goldenring, Richard J DiPaolo
Background & Aims: Atrophic gastritis caused by chronic inflammation in the gastric mucosa leads to the loss of gastric glandular cells, including acid-secreting parietal cells. Parietal cell atrophy in a setting of chronic inflammation induces spasmolytic polypeptide expressing metaplasia, a critical step in gastric carcinogenesis. However, the mechanisms by which inflammation causes parietal cell atrophy and spasmolytic polypeptide expressing metaplasia are not well defined. We investigated the role of interleukin-17A (IL-17A) in causing parietal cell atrophy...
2018: Cellular and Molecular Gastroenterology and Hepatology
Federica Tarquini, Elena Picchiassi, Giuliana Coata, Michela Centra, Vittorio Bini, Samanta Meniconi, Chiara Antonelli, Irene Giardina, Gian Carlo Di Renzo
The purpose of the present study was to search for associations between spontaneous preterm birth (sPTB), single nucleotide polymorphisms (SNPs) associated with the apoptotic pathway as triggered by oxidative stress, maternal lifestyle and health status. SNP genotyping [rs7560 for c-Jun N-terminal kinase (JNK), rs9517320 for mammalian STE20-like protein kinase 3 (MST3), rs1049216 for caspase 3 (CASP3)] in the placenta and maternal blood of 300 controls with at-term birth and 43 cases of sPTB was performed. No association was identified in genotype frequencies or combinations of foetal/maternal genotypes between single SNPs and sPTB...
July 2018: Biomedical Reports
Matias Eliseo Melendez, Renato José Silva-Oliveira, Anna Luiza Silva Almeida Vicente, Lidia Maria Rebolho Batista Arantes, Ana Carolina de Carvalho, Alberto Luis Epstein, Rui Manuel Reis, André Lopes Carvalho
Apoptosis induction has emerged as a treatment option for anticancer therapy. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a type II transmembrane protein, is a potent and specific pro-apoptotic protein ligand, which activates the extrinsic apoptosis pathway of the cell death receptors. Here we describe the construction and characterization of a new soluble TRAIL, sfTRAIL, stabilized with the trimerization Foldon domain from the Fibritin protein of the bacteriophage T4. Supernatants of 0...
June 5, 2018: Oncotarget
Abdel Aouacheria, Kyle W Cunningham, J Marie Hardwick, Zdena Palková, Ted Powers, Fedor F Severin, Libuše Váchová
Shlezinger et al (Reports, 8 September 2017, p. 1037) report that the common fungus Aspergillus fumigatus , a cause of aspergillosis, undergoes caspase-dependent apoptosis-like cell death triggered by lung neutrophils. However, the technologies they used do not provide reliable evidence that fungal cells die via a protease signaling cascade thwarted by a fungal caspase inhibitor homologous to human survivin.
June 22, 2018: Science
Michael G Kattah, Ling Shao, Yenny Y Rosli, Hiromichi Shimizu, Michael I Whang, Rommel Advincula, Philip Achacoso, Sanjana Shah, Bao H Duong, Michio Onizawa, Priscilia Tanbun, Barbara A Malynn, Averil Ma
A20 ( TNFAIP3 ) and ABIN-1 ( TNIP1 ) are candidate susceptibility genes for inflammatory bowel disease and other autoimmune or inflammatory diseases, but it is unclear how these proteins interact in vivo to prevent disease. Here we show that intestinal epithelial cell (IEC)-specific deletion of either A20 or ABIN-1 alone leads to negligible IEC loss, whereas simultaneous deletion of both A20 and ABIN-1 leads to rapid IEC death and mouse lethality. Deletion of both A20 and ABIN-1 from enteroids causes spontaneous cell death in the absence of microbes or hematopoietic cells...
June 21, 2018: Journal of Experimental Medicine
Yvan Jamilloux, Brice Lagrange, Antonia Di MIcco, Emilie Bourdonnay, Angélina Provost, Rémy Tallant, Thomas Henry, Fabio Martinon
The inflammasome is a major component of the innate immune system, and its main function is to activate caspase-1, a cysteine protease that promotes inflammation by inducing interleukin-1β (IL-1β) maturation and release into the extracellular milieu. To prevent uncontrolled inflammation, this complex is highly regulated. Once it is assembled, the inflammasome is insoluble, which has long precluded the analysis of its interactions with other proteins. Here, we used the proximity-dependent biotinylation assay (BioID) to identify proteins associated with caspase-1 during inflammasome activation...
June 21, 2018: Journal of Biological Chemistry
Fenqin Chen, Guozhu Wei, Jiao Xu, Xiaoyu Ma, Qiuyue Wang
BACKGROUND: The Nucleotide binding and oligomerization domain-like receptorfamily pyrin domain-containing 3 (NLRP3)-inflammasome plays an important role in various diseases, including a variety of kidney diseases. Naringin exhibits anti-inflammatory and anti-oxidation effects among others, but its specific mechanisms are not clear. We investigated the expression of the NLRP3-inflammasome under high-glucose conditions, assessed the effects of naringin on that process, and further elucidated the role of naringin in the pathogenesis of diabetic kidney disease(DKD)...
June 22, 2018: BMC Complementary and Alternative Medicine
Liang Xie, Songqing He, Na Kong, Ying Zhu, Yi Tang, Jianhua Li, Zhengbing Liu, Jing Liu, Jianbin Gong
BACKGROUND/AIMS: Toll-like receptors (TLRs) have been implicated in myocardial ischemia/ reperfusion (I/R) injury. We examined the effect of CpG-oligodeoxynucleotide (ODN) on myocardial I/R injury. METHODS: Male Sprague-Dawley rats were treated with either CpG-ODN or control ODN 1 h prior to myocardial ischemia (30 min) followed by reperfusion. Rats treated with phosphate-buffered saline (PBS) served as I/R controls (n = 8/group). Infarct size was determined by 2,3,5-triphenyltetrazolium chloride and Evans blue straining...
June 19, 2018: Cellular Physiology and Biochemistry
Yu Zhang, Weida Gao, Kongbin Yang, Haiquan Tao, Haicheng Yang
BACKGROUND/AIMS: Alcohol consumption has been shown to cause neuroinflammation and increase a variety of immune-related signaling processes. Microglia are a crucial part of alcohol-induced neuroinflammation and undergo apoptosis. Even though the importance of these inflammatory processes in the effects of alcohol-related neurodegeneration have been established, the mechanism of alcohol-induced microglia apoptosis is unknown. In prior research, we discovered that alcohol increases expression of salt-inducible kinase 1 (SIK1) in rodent brain tissue...
June 19, 2018: Cellular Physiology and Biochemistry
Pan Lu, Shan Lei, Weisong Li, Yang Lu, Juan Zheng, Ning Wang, Yongjun Xia, Haixia Lu, Xinlin Chen, Yong Liu, Peng-Bo Zhang
BACKGROUND/AIMS: Ketamine inhibits the proliferation of neural stem cells (NSCs) and disturbs normal neurogenesis. Dexmedetomidine provides neuroprotection against volatile anesthetic-induced neuroapoptosis and cognitive impairment in the developing brain. Whether it may protect NSCs from ketamine-induced injury remains unknown. In this study, we investigated the protective effects of dexmedetomidine on ketamine-exposed NSCs and explored the mechanisms potentially involved. METHODS: Primary NSC cultures were characterized using immunofluorescence...
June 19, 2018: Cellular Physiology and Biochemistry
Upendra K Sharma, Dinesh Mohanakrishnan, Nandini Sharma, Danish Equbal, Dinkar Sahal, Arun K Sinha
The inherent affinity of natural compounds for biological receptors has been comprehensively exploited with great success for the development of many drugs, including antimalarials. Here the natural flavoring compound vanillin has been used as an economical precursor for the synthesis of a series of novel bischalcones whose in vitro antiplasmodial activities have been evaluated against erythrocytic stages of Plasmodium falciparum. Bischalcones 9, 11 and 13 showed promising antiplasmodial activity {Chloroquine (CQ) sensitive Pf3D7 IC50 (μM): 2...
June 15, 2018: European Journal of Medicinal Chemistry
Mohammad Rasheduzzaman, Ji-Hong Moon, Ju-Hee Lee, Uddin Md Nazim, Sang-Youel Park
Telmisartan broadly used for the treatment of hypertension that is also known for its anticancer properties. TRAIL has the potential to kill tumor cells with minimal toxicity in normal cells by binding to death receptors, DR4 and DR5. Unfortunately, these TRAIL-death receptors have failed as most human cancers are resistant to TRAIL-mediated apoptosis. In this study, we evaluated telmisartan as a novel TRAIL-DR5-targeting agent with the aim of rendering TRAIL-based cancer therapies more active. Herein, we demonstrated that telmisartan could sensitize TRAIL and enhance NSCLC tumor cell death...
June 18, 2018: International Journal of Biochemistry & Cell Biology
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