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https://www.readbyqxmd.com/read/28074058/intracellular-atp-in-balance-of-pro-and-anti-inflammatory-cytokines-in-adipose-tissue-with-and-without-tissue-expansion
#1
J H Lee, Y Zhang, Z Zhao, X Ye, X Zhang, H Wang, J Ye
OBJECTIVES: To understand the mechanism of white fat expansion in the presence of inflammation, we examined the balance of pro- and anti-inflammatory cytokines in epididymal fat during weight gain in DIO mice. METHODS: The pro- and anti-inflammatory cytokines were examined in white fat of diet-induced obese mice and lean mice. The mechanism of gene expression was investigated with a focus on intracellular ATP (iATP). ATP activity was tested in cellular and non-cellular systems in activation of serine kinases (IKKβ, JNK and ERK)...
January 11, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/27988246/identification-of-neuronal-and-angiogenic-growth-factors-in-an-in-vitro-blood-brain-barrier-model-system-relevance-in-barrier-integrity-and-tight-junction-formation-and-complexity
#2
Christian Freese, Sanshiro Hanada, Petra Fallier-Becker, C James Kirkpatrick, Ronald E Unger
We previously demonstrated that the co-cultivation of endothelial cells with neural cells resulted in an improved integrity of the in vitro blood-brain barrier (BBB), and that this model could be useful to evaluate the transport properties of potential central nervous system disease drugs through the microvascular brain endothelial. In this study we have used real-time PCR, fluorescent microscopy, protein arrays and enzyme-linked immunosorbent assays to determine which neural- and endothelial cell-derived factors are produced in the co-culture and improve the integrity of the BBB...
December 15, 2016: Microvascular Research
https://www.readbyqxmd.com/read/27973457/pedf-inhibits-the-activation-of-nlrp3-inflammasome-in-hypoxia-cardiomyocytes-through-pedf-receptor-phospholipase-a2
#3
Zhongxin Zhou, Zhu Wang, Qiuhua Guan, Fan Qiu, Yufeng Li, Zhiwei Liu, Hao Zhang, Hongyan Dong, Zhongming Zhang
The nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome has been linked to sterile inflammation, which is involved in ischemic injury in myocardial cells. Pigment epithelium-derived factor (PEDF) is a multifunctional secreted glycoprotein with many biological activities, such as anti-inflammatory, antioxidant and anti-angiogenic properties. However, it is not known whether and how PEDF acts to regulate the activation of the NLRP3 inflammasome in cardiomyocytes. In the present study, we used the neonatal cardiomyocytes models of ischemia-like conditions to evaluate the mitochondrial fission and the activation of the NLRP3 inflammasome...
December 12, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27936049/clinicopathological-features-and-prognosis-of-papillary-thyroid-microcarcinoma-for-surgery-and-relationships-with-the-brafv600e-mutational-status-and-expression-of-angiogenic-factors
#4
Chenlei Shi, Yong Guo, Yichen Lv, Abiyasi Nanding, Tiefeng Shi, Huadong Qin, Jianjun He
OBJECTIVE: To investigate the clinicopathological characteristics of papillary thyroid microcarcinoma (PTMC) for surgery by comparing the difference between PTMC and larger papillary thyroid carcinoma (LPTC). METHODS: We analyzed the differences in the clinicopathological characteristics, prognosis, B-type RAF kinase (BRAF)V600E mutational status and expression of angiogenic factors, including pigment epithelium-derived factor (PEDF), Vascular Endothelial Growth Factor (VEGF), and hypoxia-inducible factor alpha subunit (HIF-1α), between PTMC and LPTC by retrospectively reviewing the records of 251 patients with papillary thyroid carcinoma, 169 with PTMC, and 82 with LPTC (diameter >1 cm)...
2016: PloS One
https://www.readbyqxmd.com/read/27931509/transplantation-of-human-urine-derived-stem-cells-transfected-with-pigment-epithelium-derived-factor-to-protect-erectile-function-in-a-rat-model-of-cavernous-nerve-injury
#5
Qiyun Yang, Xin Chen, Tao Zheng, Dayu Han, Heng Zhang, Yanan Shi, Jun Bian, Xiangzhou Sun, Kai Xia, Xiaoyan Liang, Guihua Liu, Yuanyuan Zhang, Chunhua Deng
The aim of this study was to investigate whether intracavernous injection of urine-derived stem cells (USCs) or USCs genetically modified with pigment epithelium-derived factor (PEDF) could protect the erectile function and cavernous structure in a bilateral cavernous nerve injury-induced erectile dysfunction (CNIED) rat model. USCs were cultured from the urine of six healthy male donors. Seventy-five rats were randomly divided into five groups (n=15 per group): sham, bilateral cavernous nerve (CN) crush injury (BCNI), USC, USCGFP+, and USCGFP/PEDF+ groups...
November 2016: Cell Transplantation
https://www.readbyqxmd.com/read/27914705/pigment-epithelium-derived-factor-a-noninhibitory-serine-protease-inhibitor-is-renoprotective-by-inhibiting-the-wnt-pathway
#6
Xuemin He, Rui Cheng, Kyoungmin Park, Siribhinya Benyajati, Gennadiy Moiseyev, Chengyi Sun, Lorin E Olson, Yanhui Yang, Bonnie K Eby, Kai Lau, Jian-Xing Ma
Pigment epithelium-derived factor (PEDF) expression is downregulated in the kidneys of diabetic rats, and delivery of PEDF suppressed renal fibrotic factors in these animals. PEDF has multiple functions including anti-angiogenic, anti-inflammatory and antifibrotic activities. Since the mechanism underlying its antifibrotic effect remains unclear, we studied this in several murine models of renal disease. Renal PEDF levels were significantly reduced in genetic models of type 1 and type 2 diabetes (Akita and db/db, respectively), negatively correlating with Wnt signaling activity in the kidneys...
November 30, 2016: Kidney International
https://www.readbyqxmd.com/read/27855634/identification-of-novel-targets-of-diabetic-nephropathy-and-pedf-peptide-treatment-using-rna-seq
#7
Ana Rubin, Anna C Salzberg, Yuka Imamura, Anzor Grivitishvilli, Joyce Tombran-Tink
BACKGROUND: Diabetic nephropathy (DN) is a major complication of type1 and type 2 diabetes. Understanding how diabetes regulate transcriptome dynamics in DN is important for understanding the biology of the disease and for guiding development of new treatments. RESULTS: We analyzed the kidney transcriptome of a DN mouse model, D2.B6-Ins2 (Akita) /MatbJ, before/after treatment with P78-PEDF. Age, weight, and gender-matched mice and wild-type (wt) littermates were treated at 6 weeks (early treatment) or 12 weeks (late treatment) of age for the duration of 6 weeks...
November 17, 2016: BMC Genomics
https://www.readbyqxmd.com/read/27820950/the-ingenious-interactions-between-macrophages-and-functionally-plastic-retinal-pigment-epithelium-cells
#8
Takahiro Yamawaki, Eiko Ito, Atsushi Mukai, Morio Ueno, Jun Yamada, Chie Sotozono, Shigeru Kinoshita, Junji Hamuro
Purpose: The purpose of this study was to clarify the interactions between macrophages (MPs) and RPE cells in coculture systems to investigate the functional plasticity of RPE cells. Methods: Adherent peritoneal cells or murine MP cell line Raw 264.7 was cocultured with primary RPE cells taken from C57BL/6 mice, with or without lipopolysaccharide (LPS) or TNF-α stimulation. The cytokine levels of the culture supernatants (CSs) were then analyzed with the Bio-Plex murine 23-Plex Panel Assay Kit (Bio-Rad Laboratories)...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27796462/novel-mutations-in-serpinf1-result-in-rare-osteogenesis-imperfecta-type-vi
#9
Jian-Yi Wang, Yi Liu, Li-Jie Song, Fang Lv, Xiao-Jie Xu, A San, Jian Wang, Huan-Ming Yang, Zi-Ying Yang, Yan Jiang, Ou Wang, Wei-Bo Xia, Xiao-Ping Xing, Mei Li
Osteogenesis imperfecta (OI) is a group of inherited disorders characterized by recurrent fragile fractures. Serpin peptidase inhibitor, clade F, member 1 (SERPINF1) is known to cause a distinct, extremely rare autosomal recessive form of type VI OI. Here we report, for the first time, the detection of SERPINF1 mutations in Chinese OI patients. We designed a novel targeted next-generation sequencing panel of OI-related genes to identify pathogenic mutations, which were confirmed with Sanger sequencing and by co-segregation analysis...
October 28, 2016: Calcified Tissue International
https://www.readbyqxmd.com/read/27748324/pigment-epithelium-derived-factor-and-matrix-metalloproteinase-9-in-liver-cirrhosis
#10
Joanna Kozlowska, Tomasz Mikula, Magdalena Suchacz, Joanna Jabnonska, Wojciech Stanczak, Janusz Cianciara, Alicja Wiercinska-Drapalo
BACKGROUND/AIM: The aim of this study was to assess the role of serum pigment epithelium-derived factor (PEDF) and matrix metalloproteinase-9 (MMP-9) in progression of liver cirrhosis and development of hepatocellular carcinoma (HCC). PATIENTS AND METHODS: Serum levels of PEDF and MMP-9 were tested in 212 patients with liver cirrhosis and in a control group of 30 healthy volunteers. HCC was diagnosed in 45 of the 212 patients studied (21%). RESULTS: Serum PEDF and MMP-9 were higher in the study group than that in the control group (P < 0...
September 2016: Saudi Journal of Gastroenterology: Official Journal of the Saudi Gastroenterology Association
https://www.readbyqxmd.com/read/27747237/c3a-increases-vegf-and-decreases-pedf-mrna-levels-in-human-retinal-pigment-epithelial-cells
#11
Qin Long, Xiaoguang Cao, Ailing Bian, Ying Li
Complement activation, specifically complement 3 (C3) activation and C3a generation, contributes to an imbalance between angiogenic stimulation by vascular endothelial growth factor (VEGF) and angiogenic inhibition by pigment epithelial derived factor (PEDF), leading to pathological angiogenesis. This study aimed to investigate the effects of C3a and small interfering RNA (siRNA) targeting C3 on the levels of VEGF and PEDF mRNAs in human retinal pigment epithelial (RPE) cells. ARPE-19 cells were cultured in the presence of exogenous C3a at 0...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27716557/circulating-level-of-pigment-epithelium-derived-factor-is-associated-with-vascular-function-and-structure-a-cross-sectional-study
#12
Masato Kajikawa, Tatsuya Maruhashi, Yumiko Iwamoto, Akimichi Iwamoto, Nozomu Oda, Shinji Kishimoto, Shogo Matsui, Yoshiki Aibara, Takayuki Hidaka, Yasuki Kihara, Kazuaki Chayama, Chikara Goto, Kensuke Noma, Ayumu Nakashima, Takanori Matsui, Sho-Ichi Yamagishi, Yukihito Higashi
BACKGROUND: Pigment epithelium-derived factor (PEDF) is a glycoprotein that belongs to the superfamily of serine protease inhibitors. It is thought that PEDF plays a protective role against atherosclerosis. Clinical studies have shown that serum levels of PEDF are increased in subjects with cardiovascular risk factors. The role of PEDF in cardiovascular disease is still controversial. The purpose of this study was to evaluate the associations between serum levels of PEDF and vascular function and structure...
October 3, 2016: International Journal of Cardiology
https://www.readbyqxmd.com/read/27683034/intraglomerular-crosstalk-elaborately-regulates-podocyte-injury-and-repair-in-diabetic-patients-insights-from-a-3d-multiscale-modeling-study
#13
Hua Tan, Hualin Yi, Weiling Zhao, Jian-Xing Ma, Yuanyuan Zhang, Xiaobo Zhou
Podocytes are mainly involved in the regulation of glomerular filtration rate (GFR) under physiological condition. Podocyte depletion is a crucial pathological alteration in diabetic nephropathy (DN) and results in a broad spectrum of clinical syndromes such as protein urine and renal insufficiency. Recent studies indicate that depleted podocytes can be regenerated via differentiation of the parietal epithelial cells (PECs), which serve as the local progenitors of podocytes. However, the podocyte regeneration process is regulated by a complicated mechanism of cell-cell interactions and cytokine stimulations, which has been studied in a piecemeal manner rather than systematically...
September 24, 2016: Oncotarget
https://www.readbyqxmd.com/read/27680872/the-dual-role-of-pedf-in-the-pathogenesis-of-ohss-negating-both-angiogenic-and-inflammatory-pathways
#14
Irit Miller, Dana Chuderland, Hadas Grossman, Raphael Ron-El, Ido Ben-Ami, Ruth Shalgi
CONTEXT: Ovarian hyperstimulation syndrome (OHSS) is a potentially life-threatening complication of assisted reproductive technologies. This complex syndrome is known to involve massive angiogenesis and inflammation. We have previously established the anti-angiogenic involvement of pigment epithelium-derived factor (PEDF) in the pathophysiology and treatment of OHSS. OBJECTIVE: Evaluate the anti-inflammatory role of PEDF in OHSS. DESIGN: In vivo mouse OHSS model and in vitro cultures of granulosa cells...
December 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27648638/lysophosphatidic-acids-and-autotaxin-in-retinal-vein-occlusion
#15
Ivanka Dacheva, Christoph Ullmer, Karolina Ceglowska, Everson Nogoceke, Guido Hartmann, Stephan Müller, Robert Rejdak, Katarzyna Nowomiejska, Michael Reich, Matthias Nobl, Tamer Tandogan, Florian T A Kretz, Gerd U Auffarth, Michael J Koss
PURPOSE: To analyze the levels of lysophosphatidic acids (LPAs) and autotaxin (ATX) in undiluted vitreous of untreated patients with retinal vein occlusion (RVO). METHODS: Sixty-four vitreous samples (40 RVO, 24 controls with idiopathic floaters) were analyzed in this retrospective case series using LC/MS for LPAs 16:0, 18:0, 18:1, 20:4, and ELISA kits or Luminex technology for ATX, angiopoetin-1 (ANG-1), interleukin-6 (IL-6), interleukin-7 (IL-7), interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), pigment epithelium-derived factor (PEDF), and vascular endothelial growth factor (VEGF)...
December 2016: Retina
https://www.readbyqxmd.com/read/27644257/downregulation-of-pigment-epithelium-derived-factor-is-associated-with-increased-epithelial-mesenchymal-transition-emt-in-bladder-cancer
#16
Bin B Dong, Jia S Yan, Yang Y Yan, Tian C Xie, Liang Xu, Guang H Hu, Yun F Xu, Min Liu
BACKGROUND: Pigment epithelium-derived factor (PEDF) has been reported to inhibit angiogenesis in bladder cancer, but whether it modulates epithelial-mesenchymal transition (EMT) remains unknown. METHODS: We have included 68 samples of non-muscle invasive bladder cancer in this study. Immunohistochemistry was performed in all sample sections for expressions of PEDF, SNAI2, Vimentin and E-cadherin, which were quantified and The EMT index (EMTi) was calculated by Vimentin/E-cadherin...
September 20, 2016: Panminerva Medica
https://www.readbyqxmd.com/read/27635633/a-novel-inhibitor-of-5-lipoxygenase-5-lox-prevents-oxidative-stress-induced-cell-death-of-retinal-pigment-epithelium-rpe-cells
#17
Preeti Subramanian, Emily F Mendez, S Patricia Becerra
PURPOSE: 5-Lipoxygenase (5-LOX) oxygenates arachidonic acid to form 5-hydroperoxyeicosatetraenoic acid, which is further converted into biologically detrimental leukotrienes, such as leukotriene B4 (LTB4). The RPE and retina express the PNPLA2 gene for pigment epithelium-derived factor receptor (PEDF-R), a lipase involved in cell survival. The purpose here was to investigate the role of PEDF-R on the 5-LOX pathway in oxidative stress of RPE. METHODS: Lipoxygenase activity assays were performed with soybean and potato lipoxygenase...
September 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27616658/the-significance-of-the-increased-expression-of-phosphorylated-mecp2-in-the-membranes-from-patients-with-proliferative-diabetic-retinopathy
#18
Xiaohua Li, Xiaohui Liu, Haoyi Guo, Zhaoxia Zhao, Yun Sui Li, Guoming Chen
The purpose of this study was to evaluate the correlation of expression of phosphorylated methyl-CpG binding protein 2-Ser421 (MeCP2-S421) and VEGF in the membranes of patients with PDR. We examined the expression of phospho-MeCP2-S80, S421, VEGF and PEDF in surgically excised PDR membranes from 33 patients with diabetes, and idiopathic epiretinal membranes from 11 patients without diabetes, using immunohistochemistry and western blot. The colocalization of MeCP2-S421 with VEGF, PEDF, CD31, GFAP and αSMA was revealed by fluorescent double labeling...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27615739/combined-tumor-and-neovascular-dual-targeting-gene-chemo-therapy-suppresses-tumor-growth-and-angiogenesis
#19
Bei Xu, Quansheng Jin, Jun Zeng, Ting Yu, Yan Chen, Shuangzhi Li, Daoqiong Gong, Lili He, Xiaoyue Tan, Li Yang, Gu He, Jinhui Wu, Xiangrong Song
A rational combination is critical to achieve efficiently synergistic therapeutic efficacy for tumor treatment. Hence, we designed novel antitumor combinations (T-NPs) by integrating the tumor vascular and tumor cells dual-targeting ligand with antiangiogenesis/antitumor agents. The truncated bFGF peptide (tbFGF), which could effectively bind to FGFR1 overexpressed on tumor neovasculature endothelial cells and tumor cells, was selected to modify PLGA nanoparticles (D/P-NPs) simultaneously loaded with PEDF gene and paclitaxel in this study...
October 5, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27590659/abnormal-pigment-epithelium-derived-factor-processing-in-progressive-myopia
#20
Natalya I Minkevich, Ludmilla A Morozova-Roche, Elena N Iomdina, Tatiana V Rakitina, Anna P Bogachuk, Dmitry L Kakuev, Evgeniya V Smirnova, Igor I Babichenko, Valery M Lipkin
Pigment Epithelium-Derived Factor (PEDF) is a secreted glycoprotein belonging to the family of non-inhibitory serpins. It is known, that in cases of complicated myopia, the content of PEDF in aqueous humor of the anterior chamber is significantly reduced. Here we examined a bulk of Tenon's capsule samples obtained from various groups of myopes, to examine PEDF processing in progressive myopia. We have analyzed the distribution of full length PEDF50 and its truncated form PEDF45 in the soluble and insoluble fractions extracted from Tenon's capsule of myopic and control (non-myopic) patients using SDS-polyacrylamide gel electrophoresis, as well as monitored the proteolytic degradation of PEDF ex vivo by enzyme-linked immunosorbent assay...
August 30, 2016: Experimental Eye Research
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