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Joanna Kozlowska, Tomasz Mikula, Magdalena Suchacz, Joanna Jabnonska, Wojciech Stanczak, Janusz Cianciara, Alicja Wiercinska-Drapalo
BACKGROUND/AIM: The aim of this study was to assess the role of serum pigment epithelium-derived factor (PEDF) and matrix metalloproteinase-9 (MMP-9) in progression of liver cirrhosis and development of hepatocellular carcinoma (HCC). PATIENTS AND METHODS: Serum levels of PEDF and MMP-9 were tested in 212 patients with liver cirrhosis and in a control group of 30 healthy volunteers. HCC was diagnosed in 45 of the 212 patients studied (21%). RESULTS: Serum PEDF and MMP-9 were higher in the study group than that in the control group (P < 0...
September 2016: Saudi Journal of Gastroenterology: Official Journal of the Saudi Gastroenterology Association
Qin Long, Xiaoguang Cao, Ailing Bian, Ying Li
Complement activation, specifically complement 3 (C3) activation and C3a generation, contributes to an imbalance between angiogenic stimulation by vascular endothelial growth factor (VEGF) and angiogenic inhibition by pigment epithelial derived factor (PEDF), leading to pathological angiogenesis. This study aimed to investigate the effects of C3a and small interfering RNA (siRNA) targeting C3 on the levels of VEGF and PEDF mRNAs in human retinal pigment epithelial (RPE) cells. ARPE-19 cells were cultured in the presence of exogenous C3a at 0...
2016: BioMed Research International
Masato Kajikawa, Tatsuya Maruhashi, Yumiko Iwamoto, Akimichi Iwamoto, Nozomu Oda, Shinji Kishimoto, Shogo Matsui, Yoshiki Aibara, Takayuki Hidaka, Yasuki Kihara, Kazuaki Chayama, Chikara Goto, Kensuke Noma, Ayumu Nakashima, Takanori Matsui, Sho-Ichi Yamagishi, Yukihito Higashi
BACKGROUND: Pigment epithelium-derived factor (PEDF) is a glycoprotein that belongs to the superfamily of serine protease inhibitors. It is thought that PEDF plays a protective role against atherosclerosis. Clinical studies have shown that serum levels of PEDF are increased in subjects with cardiovascular risk factors. The role of PEDF in cardiovascular disease is still controversial. The purpose of this study was to evaluate the associations between serum levels of PEDF and vascular function and structure...
October 3, 2016: International Journal of Cardiology
Hua Tan, Hualin Yi, Weiling Zhao, Jian-Xing Ma, Yuanyuan Zhang, Xiaobo Zhou
Podocytes are mainly involved in the regulation of glomerular filtration rate (GFR) under physiological condition. Podocyte depletion is a crucial pathological alteration in diabetic nephropathy (DN) and results in a broad spectrum of clinical syndromes such as protein urine and renal insufficiency. Recent studies indicate that depleted podocytes can be regenerated via differentiation of the parietal epithelial cells (PECs), which serve as the local progenitors of podocytes. However, the podocyte regeneration process is regulated by a complicated mechanism of cell-cell interactions and cytokine stimulations, which has been studied in a piecemeal manner rather than systematically...
September 24, 2016: Oncotarget
Irit Miller, Dana Chuderland, Hadas Grossman, Raphael Ron-El, Ido Ben-Ami, Ruth Shalgi
CONTEXT: Ovarian hyperstimulation syndrome (OHSS) is a potentially life-threatening complication of assisted reproductive technologies (ART).This complex syndrome is known to involve massive angiogenesis and inflammation. We have previously established the anti-angiogenic involvement of pigment epithelium-derived factor (PEDF) in the pathophysiology and treatment of OHSS. OBJECTIVE: Evaluate the anti-inflammatory role of PEDF in OHSS. DESIGN: In-vivo mouse OHSS-model and in-vitro cultures of granulosa cells...
September 28, 2016: Journal of Clinical Endocrinology and Metabolism
Ivanka Dacheva, Christoph Ullmer, Karolina Ceglowska, Everson Nogoceke, Guido Hartmann, Stephan Müller, Robert Rejdak, Katarzyna Nowomiejska, Michael Reich, Matthias Nobl, Tamer Tandogan, Florian T A Kretz, Gerd U Auffarth, Michael J Koss
PURPOSE: To analyze the levels of lysophosphatidic acids (LPAs) and autotaxin (ATX) in undiluted vitreous of untreated patients with retinal vein occlusion (RVO). METHODS: Sixty-four vitreous samples (40 RVO, 24 controls with idiopathic floaters) were analyzed in this retrospective case series using LC/MS for LPAs 16:0, 18:0, 18:1, 20:4, and ELISA kits or Luminex technology for ATX, angiopoetin-1 (ANG-1), interleukin-6 (IL-6), interleukin-7 (IL-7), interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), pigment epithelium-derived factor (PEDF), and vascular endothelial growth factor (VEGF)...
July 12, 2016: Retina
Bin B Dong, Jia S Yan, Yang Y Yan, Tian C Xie, Liang Xu, Guang H Hu, Yun F Xu, Min Liu
BACKGROUND: Pigment epithelium-derived factor (PEDF) has been reported to inhibit angiogenesis in bladder cancer, but whether it modulates epithelial-mesenchymal transition (EMT) remains unknown. METHODS: We have included 68 samples of non-muscle invasive bladder cancer in this study. Immunohistochemistry was performed in all sample sections for expressions of PEDF, SNAI2, Vimentin and E-cadherin, which were quantified and The EMT index (EMTi) was calculated by Vimentin/E-cadherin...
September 20, 2016: Panminerva Medica
Preeti Subramanian, Emily F Mendez, S Patricia Becerra
PURPOSE: 5-Lipoxygenase (5-LOX) oxygenates arachidonic acid to form 5-hydroperoxyeicosatetraenoic acid, which is further converted into biologically detrimental leukotrienes, such as leukotriene B4 (LTB4). The RPE and retina express the PNPLA2 gene for pigment epithelium-derived factor receptor (PEDF-R), a lipase involved in cell survival. The purpose here was to investigate the role of PEDF-R on the 5-LOX pathway in oxidative stress of RPE. METHODS: Lipoxygenase activity assays were performed with soybean and potato lipoxygenase...
September 1, 2016: Investigative Ophthalmology & Visual Science
Xiaohua Li, Xiaohui Liu, Haoyi Guo, Zhaoxia Zhao, Yun Sui Li, Guoming Chen
The purpose of this study was to evaluate the correlation of expression of phosphorylated methyl-CpG binding protein 2-Ser421 (MeCP2-S421) and VEGF in the membranes of patients with PDR. We examined the expression of phospho-MeCP2-S80, S421, VEGF and PEDF in surgically excised PDR membranes from 33 patients with diabetes, and idiopathic epiretinal membranes from 11 patients without diabetes, using immunohistochemistry and western blot. The colocalization of MeCP2-S421 with VEGF, PEDF, CD31, GFAP and αSMA was revealed by fluorescent double labeling...
2016: Scientific Reports
Bei Xu, Quansheng Jin, Jun Zeng, Ting Yu, Yan Chen, Shuangzhi Li, Daoqiong Gong, Lili He, Xiaoyue Tan, Li Yang, Gu He, Jinhui Wu, Xiangrong Song
A rational combination is critical to achieve efficiently synergistic therapeutic efficacy for tumor treatment. Hence, we designed novel antitumor combinations (T-NPs) by integrating the tumor vascular and tumor cells dual-targeting ligand with antiangiogenesis/antitumor agents. The truncated bFGF peptide (tbFGF), which could effectively bind to FGFR1 overexpressed on tumor neovasculature endothelial cells and tumor cells, was selected to modify PLGA nanoparticles (D/P-NPs) simultaneously loaded with PEDF gene and paclitaxel in this study...
October 5, 2016: ACS Applied Materials & Interfaces
Natalya I Minkevich, Ludmilla A Morozova-Roche, Elena N Iomdina, Tatiana V Rakitina, Anna P Bogachuk, Dmitry L Kakuev, Evgeniya V Smirnova, Igor I Babichenko, Valery M Lipkin
Pigment Epithelium-Derived Factor (PEDF) is a secreted glycoprotein belonging to the family of non-inhibitory serpins. It is known, that in cases of complicated myopia, the content of PEDF in aqueous humor of the anterior chamber is significantly reduced. Here we examined a bulk of Tenon's capsule samples obtained from various groups of myopes, to examine PEDF processing in progressive myopia. We have analyzed the distribution of full length PEDF50 and its truncated form PEDF45 in the soluble and insoluble fractions extracted from Tenon's capsule of myopic and control (non-myopic) patients using SDS-polyacrylamide gel electrophoresis, as well as monitored the proteolytic degradation of PEDF ex vivo by enzyme-linked immunosorbent assay...
August 30, 2016: Experimental Eye Research
Ya-Jie Yu, Bin Mo, Lu Liu, Yan-Kun Yue, Chang-Li Yue, Wu Liu
AIM: To evaluate the effects of lentivirus-mediated pigment epithelium-derived factor (PEDF) gene transfer performed in treatment of rats with established choroidal neovascularization (CNV), and investigates the mechanism by which PEDF inhibits CNV in rats. METHODS: Brown Norway (BN) rats (n=204) were induced by exposure to a laser, and then randomly assigned to 3 groups: no treatment; treatments with intravitreal injection of lentivirus-PEDF-green fluorescent protein (GFP) or lentivirus-control GFP (free fluorescent protein)...
2016: International Journal of Ophthalmology
Wei-Min Zhang, Zhi-Ren Zhang, Yong-Gang Zhang, Yan-Sheng Gao
BACKGROUND: Pigment epithelium-derived factor (PEDF) is regarded as a multifunctional protein possessing neurotrophic and neuroprotective properties. PEDF has a very short half-life, and it would require multiple injections to maintain a therapeutically relevant level without a delivery system. However, multiple injections are prone to cause local damage or infection. To overcome this, we chose a cell-based system that provided sustained delivery of PEDF and compared the effect of weekly injections of PEDF and neural stem cell (NSC)-based intraocular administration of PEDF on retinal ganglion cell (RGC) survival and axon regeneration after optic nerve injury...
September 2016: Iranian Journal of Medical Sciences
Glenn S Belinsky, Leanne Ward, Chuhan Chung
Osteogenesis imperfecta (OI) Type VI is characterized by a defect in bone mineralization, which results in multiple fractures early in life. Null mutations in the PEDF gene, Serpinf1, are the cause of OI VI. Whether PEDF restoration in a murine model of OI Type VI could improve bone mass and function was previously unknown. In Belinsky et al, we provided evidence that PEDF delivery enhanced bone mass and improved parameters of bone function in vivo. Further, we demonstrated that PEDF temporally inhibits Wnt signaling to enhance osteoblast differentiation...
2016: Rare Diseases
Yuhan Yang, Lili He, Yongmei Liu, Shan Xia, Aiping Fang, Yafei Xie, Li Gan, Zhiyao He, Xiaoyue Tan, Chunling Jiang, Aiping Tong, Xiangrong Song
Cervical cancer presents extremely low PEDF expression which is associated with tumor progression and poor prognosis. In this study, folate receptor α (FRα)-targeted nano-liposomes (FLP) were designed to enhance the anti-tumor effect by targeting delivery of exogenous PEDF gene to cervical cancer cells. The targeting molecule F-PEG-Chol was firstly synthesized by a novel simpler method. FLP encapsulating PEDF gene (FLP/PEDF) with a typical lipid-membrane structure were prepared by a film dispersion method...
2016: Scientific Reports
Chi-Hsien Peng, Jen-Hua Chuang, Mong-Lien Wang, Yong-Yu Jhan, Ke-Hung Chien, Yu-Chien Chung, Kuo-Hsuan Hung, Chia-Ching Chang, Chao-Kuei Lee, Wei-Lien Tseng, De-Kuang Hwang, Chia-Hsien Hsu, Tai-Chi Lin, Shih-Hwa Chiou, Shih-Jen Chen
Advanced age-related macular degeneration (AMD) may lead to geographic atrophy or fibrovascular scar at macular, dysfunctional retinal microenvironment, and cause profound visual loss. Recent clinical trials have implied the potential application of pluripotent cell-differentiated retinal pigment epithelial cells (dRPEs) and membranous scaffolds implantation in repairing the degenerated retina in AMD. However, the efficacy of implanted membrane in immobilization and supporting the viability and functions of dRPEs, as well as maintaining the retinal microenvironment is still unclear...
August 22, 2016: Oncotarget
Jingjing Gong, Glenn Belinsky, Usman Sagheer, Xuchen Zhang, Paul J Grippo, Chuhan Chung
An increase in autophagy characterizes pancreatic carcinogenesis, but the signals that regulate this process are incompletely understood. Because canonical Wnt/β-catenin signaling is necessary for the transition from early to advanced pancreatic intraepithelial neoplasia (PanIN) lesions, we assessed whether Wnt ligands and endogenous inhibitors of Wnt signaling modulate autophagy. In this study, canonical Wnt3a ligand induced autophagy markers and vacuoles in murine PanIN cells. Furthermore, pigment epithelium-derived factor (PEDF), a secreted glycoprotein known for its anti-tumor properties, blocked Wnt3a-directed induction of autophagy proteins...
October 14, 2016: Journal of Biological Chemistry
Feng Li, Gillian B Armstrong, Joyce Tombran-Tink, Christopher Niyibizi
Pigment epithelium-derived factor (PEDF) encoded by serpinf1 is a potent antiangiogenic factor found in a wide variety of fetal and adult tissues. Several reports have shown that lack of PEDF leads to osteogenesis imperfecta (OI) type VI whose hallmark is a defect in mineralization that leads to excessive osteoid build up that fails to mineralize. Because PEDF is antiangiogenic factor it would pose serious consequences on bone development and healing of fractures. To understand possible mechanisms by which PEDF plays a role in bone development and regulation of matrix mineralization, we determined the effects of exogenous PEDF on vascular endothelial growth factor (VEGF) expression by human mesenchymal stem cells (hMSCs) and mechanisms of its regulation by PEDF...
September 23, 2016: Biochemical and Biophysical Research Communications
Ben-Skowronek Iwona
Neurodegeneration is an initial process in the development of diabetic retinopathy (DR). High quantity of glutamate, oxidative stress, the induction of the renin-angiotensin system (RAS) and the elevated levels of RAGE is crucial element in the retinal neurodegeneration caused by diabetes mellitus. At least , there is emerging proofs to indicate that the equilibrium between the neurotoxic and neuroprotective components will influence on the state of the retinal neurons. Somatostatin (SST), pigment epithelium- derived factor (PEDF), erythropoietin (Epo) are endogenous neuroprotective peptides that are decreased in the eye of diabetic persons and play a essential role in retinal homeostasis...
August 13, 2016: Current Neuropharmacology
Sarvenaz Zand, Elizabeth Buzney, Lyn M Duncan, Soheil S Dadras
OBJECTIVES: Histologic and molecular heterogeneity is well recognized in malignant melanoma; however, the diversity of expression of new and classic melanoma markers has not been correlated in serial sections of metastases. METHODS: We examined and correlated the expression of microphthalmia transcription factor (MITF) with its transcriptional targets, including melastatin (MLSN1/TRPM1), pigment epithelium-derived factor (SERPINF1/PEDF), SILV/PMEL17/GP100 (human melanoma black 45 [HMB-45]), and melanoma antigen recognized by T cells 1 (MART-1)/MLANA, in 13 melanoma metastases in lymph nodes of 13 patients...
September 2016: American Journal of Clinical Pathology
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