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https://www.readbyqxmd.com/read/29351407/pedf-expression-affects-the-oxidative-and-inflammatory-state-of-choroidal-endothelial-cells
#1
Mitra Farnoodian, Christine M Sorenson, Nader Sheibani
Age related macular degeneration (AMD) is the leading cause of vision loss among the elderly population, and is associated with severe macular degeneration and choroidal neovascularization (CNV). Although the pathogenesis of AMD is associated with choroidal dysfunction and CNV, the detailed underlying mechanisms remain unresolved. Altered production of pigment epithelium derived factor (PEDF), a neuroprotective and anti-angiogenic factor, contributes to CNV. Furthermore, exogenous PEDF mitigates angiogenesis in preclinical CNV models...
January 10, 2018: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29341332/cornea-derived-mscs-therapeutically-modulate-macrophage-immunophenotype-and-angiogenic-function
#2
Medi Eslani, Ilham Putra, Xiang Shen, Judy Hamouie, Asha Tadepalli, Khandaker N Anwar, John A Kink, Samaneh Ghassemi, Gaurav Agnihotri, Sofiya Reshetylo, Alireza Mashaghi, Reza Dana, Peiman Hematti, Ali R Djalilian
Macrophages are crucial drivers of inflammatory corneal neovascularization and thus are potential targets for immunomodulatory therapies. We hypothesized that therapeutic use of cornea derived mesenchymal stromal cells (cMSCs) may alter the function of macrophages. We found that cMSCs can modulate the phenotype and angiogenic function of macrophages. In vitro, cMSCs induce apoptosis of macrophages while preferentially promoting a distinct CD14hi CD16hi CD163hi CD206hi immunophenotype that has significantly reduced angiogenic effects based on in vitro angiogenesis assays...
January 17, 2018: Stem Cells
https://www.readbyqxmd.com/read/29259049/scleroderma-fibroblasts-suppress-angiogenesis-via-tgf-%C3%AE-caveolin-1-dependent-secretion-of-pigment-epithelium-derived-factor
#3
Vasiliki Liakouli, Jacobo Elies Comez, Yasser Mohamed El-Sherbiny, Margherita Scarcia, Gary Grant, Giuseppina Abignano, Emma C Derrett-Smith, Filomena Esteves, Paola Cipriani, Paul Emery, Christopher P Denton, Roberto Giacomelli, Georgia Mavria, Francesco Del Galdo
OBJECTIVES: Systemic sclerosis (SSc) is characterised by tissue fibrosis and vasculopathy with defective angiogenesis. Transforming growth factor beta (TGF-β) plays a major role in tissue fibrosis, including downregulation of caveolin-1 (Cav-1); however, its role in defective angiogenesis is less clear. Pigment epithelium-derived factor (PEDF), a major antiangiogenic factor, is abundantly secreted by SSc fibroblasts. Here, we investigated the effect of TGF-β and Cav-1 on PEDF expression and the role of PEDF in the ability of SSc fibroblasts to modulate angiogenesis...
December 19, 2017: Annals of the Rheumatic Diseases
https://www.readbyqxmd.com/read/29258756/insulin-antagonises-pigment-epithelium-derived-factor-pedf-induced-modulation-of-lineage-commitment-of-myocytes-and%C3%A2-heterotrophic-ossification
#4
Revathy Carnagarin, Mina Elahy, Arun M Dharmarajan, Crispin R Dass
Extensive bone defects arising as a result of trauma, infection and tumour resection and other bone pathologies necessitates the identification of effective strategies in the form of tissue engineering, gene therapy and osteoinductive agents to enhance the bone repair process. PEDF is a multifunctional glycoprotein which plays an important role in regulating osteoblastic differentiation and bone formation. PEDF treatment of mice and human skeletal myocytes at physiological concentration inhibited myogenic differentiation and activated Erk1/2 MAPK- dependent osteogenic transdifferentiation of myocytes...
December 16, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/29246287/long-term-pedf-release-in-rat-iris-and-retinal-epithelial-cells-after-sleeping-beauty-transposon-mediated-gene-delivery
#5
Laura Garcia-Garcia, Sergio Recalde, Maria Hernandez, Jaione Bezunartea, Juan Roberto Rodriguez-Madoz, Sandra Johnen, Sabine Diarra, Corinne Marie, Zsuzsanna Izsvák, Zoltán Ivics, Daniel Scherman, Martina Kropp, Gabriele Thumann, Felipe Prosper, Patricia Fernandez-Robredo, Alfredo Garcia-Layana
Pigment epithelium derived factor (PEDF) is a potent antiangiogenic, neurotrophic, and neuroprotective molecule that is the endogenous inhibitor of vascular endothelial growth factor (VEGF) in the retina. An ex vivo gene therapy approach based on transgenic overexpression of PEDF in the eye is assumed to rebalance the angiogenic-antiangiogenic milieu of the retina, resulting in growth regression of choroidal blood vessels, the hallmark of neovascular age-related macular degeneration. Here, we show that rat pigment epithelial cells can be efficiently transfected with the PEDF-expressing non-viral hyperactive Sleeping Beauty transposon system delivered in a form free of antibiotic resistance marker miniplasmids...
December 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/29244789/pigment-epithelium-derived-factor-from-arpe19-promotes-proliferation-and-inhibits-apoptosis-of-human-umbilical-mesenchymal-stem-cells-in-serum-free-medium
#6
Dah-Ching Ding, Yao-Tseng Wen, Rong-Kung Tsai
Clinical expansion of mesenchymal stem cells (MSCs) is hampered by the lack of knowledge regarding how to prevent MSC apoptosis and promote their proliferation in serum-free medium. Our in vitro studies demonstrated that human umbilical cord MSCs (HUCMSCs) underwent apoptosis in the serum-free medium. When HUCMSCs were co-cultured with retinal pigment epithelial cells (ARPE19), however, HUCMSCs exhibited normal growth and morphology in serum-free medium. Their colony formation was promoted by the conditioned medium (CM) of ARPE19 cells on Matrigel...
December 15, 2017: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29210651/mir-25-mediates-retinal-degeneration-via-inhibiting-itgav-and-pedf-in-rat
#7
J Zhang, J Wang, L Zheng, M Wang, Y Lu, Z Li, C Lian, S Mao, X Hou, S Li, J Xu, H Tian, C Jin, F Gao, J Zhang, F Wang, W Li, L Lu, G-T Xu
Age-related macular degeneration (AMD) is the main cause of irreversible blindness in the elderly. Oxidative stress in retinal pigment epithelium (RPE) is deemed to play a pivotal role in the pathogenesis of AMD. miR-25 functions as an essential modulator in response to oxidative-stress in several cell types, but its function in RPE cells is poorly understood. Here, we reported that, in a rat model of retinal degeneration induced by sodium iodate (SI), oxidative stress up-regulated miR-25 in RPE cells in very early stage, accompanied by decreased phagocytosis and reduced growth factor secretion in those cells...
December 5, 2017: Current Molecular Medicine
https://www.readbyqxmd.com/read/29207076/antiangiogenic-effects-of-catalpol-on-rat-corneal%C3%A2-neovascularization
#8
Yun Han, Mei Shen, Li-Yuan Tang, Gang Tan, Qi-Chen Yang, Lei Ye, Lin-Hong Ye, Nan Jiang, Gui-Ping Gao, Yi Shao
To investigate the effects of catalpol on corneal neovascularization (CNV) and associated inflammation, eye drops (5 mM catalpol or PBS) were administered four times daily to alkali‑burn rat models of CNV and inflammation. Clinical evaluations of CNV and the degree of inflammation were performed on days 0, 4, 7, 10 and 14 under slit lamp microscopy. Eyes were collected on day 14 and prepared for hematoxylin and eosin, and immunofluorescence staining; corneal cell apoptosis was investigated via terminal deoxynucleotidyl transferase‑mediated nick end labeling (TUNEL) staining...
November 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29201190/pigment-epithelium-derived-factor-short-peptides-facilitate-full-thickness-cutaneous-wound-healing-by-promoting-epithelial-basal-cell-and-hair-follicle-stem-cell-proliferation
#9
Shawn H Tsai, Li-Pen Tsao, Shih-Hsin Chang, Tsung-Chuan Ho, Kwang-Yi Tung, Ai-Ching Wu, Yeou-Ping Tsao
A previous study by our group showed that a 44-amino-acid fragment of pigment epithelium-derived factor (PEDF) facilitated corneal epithelial wound healing. In the present study this fragment was shortened to obtain peptides of 18, 20 and 29 amino acids in length, and their promoting effects on the healing of full-thickness skin wounds were assessed. Peptides were delivered periodically by topical application to punch wounds of mice. The wound healing speed was evaluated by measuring the reduction of wound areas at 4 and 7 days after injury...
November 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29186716/m%C3%A3-ller-cell-derived-pedf-mediates-neuroprotection-via-stat3-activation
#10
Wolfram Eichler, Helena Savković-Cvijić, Susanne Bürger, Mike Beck, Manuela Schmidt, Peter Wiedemann, Andreas Reichenbach, Jan Darius Unterlauft
Background/ Aims: This study was performed to reveal signaling pathways exploited by pigment epithelium-derived factor (PEDF) derived from retinal (glial) Müller cells to protect retinal ganglion cells (RGCs) from cell death. METHODS: The survival of RGCs was determined in the presence of conditioned culture media (MCM) from or in co-cultures with Müller cells. The significance of PEDF-induced STAT3 activation was evaluated in viability assays and using Western blotting analyses and siRNA-transfected cells...
November 30, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29180961/ginsenoside-rb1-mediated-anti-angiogenesis-via-regulating-pedf-and-mir-33a-through-the-activation-of-ppar-%C3%AE-pathway
#11
Huixia Lu, Xunian Zhou, Hoi-Hin Kwok, Mei Dong, Zhaoqiang Liu, Po-Ying Poon, Xiaorong Luan, Ricky Ngok-Shun Wong
Angiogenesis is the formation of new blood vessels from the existing vasculature, which is involved in multiple biological processes, including atherosclerosis, ischemic heart disease, and cancer. Ginsenoside-Rb1 (Rb1), the most abundant ginsenoside isolated form Panax ginseng, has been identified as a promising anti-angiogenic agent via the up-regulation of PEDF. However, the underlying molecular mechanisms still unknown. In the present study, human umbilical vein endothelial cells (HUVECs) were selected to perform in vitro assays...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29177891/plasma-levels-of-hypoxia-regulated-factors-in-patients-with-age-related-macular-degeneration
#12
Zygoula Ioanna, Schori Christian, Grimm Christian, Barthelmes Daniel
PURPOSE: Various hypoxia-related proteins are differentially expressed in the retina and secreted to the vitreous and/or aqueous humor of patients affected by dry or neovascular age-related macular degeneration (nAMD). To determine whether these conditions alter concentrations of cytokines also in the systemic circulation, we measured plasma levels of six hypoxia-related proteins. METHODS: Plasma was prepared from EDTA blood that was collected from patients affected by dry AMD (n = 5), nAMD (n = 11), proliferative diabetic retinopathy (PDR; n = 9), and patients with an epiretinal membrane (ERM; n = 11)...
November 25, 2017: Graefe's Archive for Clinical and Experimental Ophthalmology
https://www.readbyqxmd.com/read/29137305/mir-93-3p-inhibition-suppresses-clear-cell-renal-cell-carcinoma-proliferation-metastasis-and-invasion
#13
Lu Wang, Guang Yang, Xiangwei Zhu, Ziqi Wang, Hongzhi Wang, Yang Bai, Pengcheng Sun, Li Peng, Wei Wei, Guang Chen, Guangbin Li, Andrey A Zamyatnin, Peter V Glybochko, Wanhai Xu
miRNA dysregulation is associated with many human diseases, including cancer. This study explored the effects of miR-93-3p on clear cell renal cell carcinoma (ccRCC). We found that miR-93-3p is upregulated an average of 38-fold in 138 ccRCC specimens compared to matched normal kidney tissues, which correlated with poor patient outcome. miR-93-3p inhibition reduced ccRCC cell growth, invasion, and migration in vitro and in a mouse xenograft model. A search of the TargetScan, miRanda, and PicTar databases revealed that miR-93-3p is predicted to regulate pigment epithelium-derived factor (PEDF)...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29117277/naringenin-eye-drops-inhibit-corneal-neovascularization-by-anti-inflammatory-and-antioxidant-mechanisms
#14
Ana P M T Oguido, Miriam S N Hohmann, Felipe A Pinho-Ribeiro, Jefferson Crespigio, Talita P Domiciano, Waldiceu A Verri, Antonio M B Casella
Purpose: To investigate the effect of naringenin eye drops in corneal neovascularization induced by alkali (1 N NaOH) burn in mice. Methods: Corneal neovascularization in the right eye of male Swiss mice was induced by alkali. Treatment with naringenin eye drops (0.08-80 μg; 8 μL of 0.01-10 g/L solution) or vehicle (saline) started 2 days before corneal neovascularization was induced and was performed twice a day. Mice were treated up until the time animals were euthanized and cornea tissue was collected for testing, which was 2, 4, and 6 hours after alkali stimulus for cytokine and antioxidant capacity measurements, and 3 and/or 7 days after alkali stimulus for the assessment of corneal epithelial thickness and neovascularization, neutrophil, and macrophage recruitment, and vascular endothelial growth factor (Vegf), platelet-derived growth factor (Pdgf), matrix metalloproteinase-14 (Mmp14), and pigment epithelium-derived factor (Pedf) mRNA expression...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29105655/pre-metastatic-cancer-exosomes-induce-immune-surveillance-by-patrolling-monocytes-at-the-metastatic-niche
#15
Michael P Plebanek, Nicholas L Angeloni, Elena Vinokour, Jia Li, Anna Henkin, Dalia Martinez-Marin, Stephanie Filleur, Reshma Bhowmick, Jack Henkin, Stephen D Miller, Igal Ifergan, Yesung Lee, Iman Osman, C Shad Thaxton, Olga V Volpert
Metastatic cancers produce exosomes that condition pre-metastatic niches in remote microenvironments to favor metastasis. In contrast, here we show that exosomes from poorly metastatic melanoma cells can potently inhibit metastasis to the lung. These "non-metastatic" exosomes stimulate an innate immune response through the expansion of Ly6C(low) patrolling monocytes (PMo) in the bone marrow, which then cause cancer cell clearance at the pre-metastatic niche, via the recruitment of NK cells and TRAIL-dependent killing of melanoma cells by macrophages...
November 6, 2017: Nature Communications
https://www.readbyqxmd.com/read/29104038/extremely-low-level-of-serum-pigment-epithelium-derived-factor-is-a-special-biomarker-of-chinese-osteogenesis-imperfecta-patients-with-serpinf1-mutations
#16
Jian-Yi Wang, Lu-Jiao Li, Qian Zhang, Yi Liu, Fang Lv, Xiao-Jie Xu, Yu-Wen Song, Ou Wang, Yan Jiang, Wei-Bo Xia, Xiao-Ping Xing, Mei Li
BACKGROUNDS: SERPINF1 mutations caused deficiency of pigment epithelium-derived factor (PEDF) and would lead to osteogenesis imperfecta (OI) type VI. However, serum PEDF levels were unclear in Chinese OI patients who had clear molecular diagnosis. OBJECTIVE: To assess PEDF levels in different genotypes of OI, to evaluate the influencing factors of PEDF in Chinese OI patients with clear molecular diagnosis. METHODS: Known candidate genes of OI were examined by a targeted next generation sequence...
November 2, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29100284/exosomes-containing-differential-expression-of-microrna-and-mrna-in-osteosarcoma-that-can-predict-response-to-chemotherapy
#17
Ji-Feng Xu, Ya-Ping Wang, Shui-Jun Zhang, Yu Chen, Hai-Feng Gu, Xiao-Fan Dou, Bing Xia, Qing Bi, Shun-Wu Fan
A major challenge in osteosarcoma (OS) is the selection of the most effective chemotherapeutic agents for individual patients, while the administration of ineffective chemotherapy increases mortality and decreases quality of life in patients. This emphasizes the need to evaluate every patient's probability of responding to each chemotherapeutic agent. We developed a profiling strategy for serum exosomal microRNAs and mRNAs in OS patients with differential chemotherapeutic responses. Twelve miRNAs were up regulated and 18 miRNAs were under regulated significantly in OS patient with poor chemotherapeutic response when compared with those in good chemotherapeutic response (p<0...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29075761/corneal-mesenchymal-stromal-cells-are-directly-antiangiogenic-via-pedf-and-sflt-1
#18
Medi Eslani, Ilham Putra, Xiang Shen, Judy Hamouie, Neda Afsharkhamseh, Soroush Besharat, Mark I Rosenblatt, Reza Dana, Peiman Hematti, Ali R Djalilian
Purpose: To evaluate the angiogenic properties of corneal derived mesenchymal stromal cells (Co-MSC). Methods: Co-MSCs were extracted from human cadaver, and wild-type (C57BL/6J) and SERPINF1-/- mice corneas. The MSC secretome was collected in a serum-free medium. Human umbilical vein endothelial cell (HUVEC) tube formation and fibrin gel bead assay (FIBA) sprout formation were used to assess the angiogenic properties of Co-MSC secretome. Complete corneal epithelial debridement was used to induce corneal neovascularization in wild-type mice...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29042415/long-term-effects-of-weight-loss-exercise-on-biomarkers-associated-with-angiogenesis
#19
Catherine Duggan, Jean de Dieu Tapsoba, Ching-Yun Wang, Karen E Foster-Schubert, Anne McTiernan
BACKGROUND: We tested the effect of weight-loss on circulating levels of the angiogenic factors vascular endothelial growth factor (VEGF), and pigment epithelium-derived factor (PEDF) in postmenopausal overweight/obese women, 18 months after completing a year-long 4-arm randomized controlled trial of behavioral weight-loss and/or exercise vs. control (i.e., 30-months post-randomization). METHODS: 439 overweight/obese, postmenopausal women, 50-75 years, were randomized to: diet (goal: 10% weight-loss, N=118), exercise (225 min/week moderate-to-vigorous activity, N=117), diet+exercise (N=117), or control (N=87)...
October 17, 2017: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/29029440/colorectal-carcinoma-with-osseous-metaplasia
#20
Xibo Liu, Jinghong Xu, Lirong Chen
Osseous metaplasia (OM) is rarely observed in colorectal cancer (incidence < 0.4% in rectal cancer), where it has a non-specific clinical presentation and unknown pathogenesis. Here, we report three cases of colorectal carcinoma with OM and propose a new hypothesis. All three patients (two males and one female) were Chinese and had different sites of colorectal carcinoma with OM: rectum, sigmoid colon, and appendix. The pathologic diagnoses were serrated adenocarcinoma; moderately to poorly differentiated adenocarcinoma with micropapillary carcinoma and cribriform comedo-type adenocarcinoma; and mucinous adenocarcinoma, respectively...
September 12, 2017: Oncotarget
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