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https://www.readbyqxmd.com/read/28911166/hormonal-and-growth-regulation-of-epithelial-and-stromal-cells-from-the-normal-and-malignant-endometrium-by-pigment-epithelium-derived-factor
#1
Julien Daubriac, Unnati M Pandya, Kuang-Tzu Huang, Savvas C Pavlides, Patricia Gama, Stephanie V Blank, Pratibha Shukla, Susan E Crawford, Leslie I Gold
We discovered that pigment epithelium-derived factor (PEDF)-null mice have endometrial hyperplasia, the precursor to human type I endometrial cancer (ECA), which is etiologically linked to unopposed estrogen (E2), suggesting that this potent antiangiogenic factor might contribute to dysregulated growth and the development of type I ECA. Treatment of both ECA cell lines and primary ECA cells with recombinant PEDF dose dependently decreased cellular proliferation via an autocrine mechanism by blocking cells in G1 and G2 phases of the cell cycle...
September 1, 2017: Endocrinology
https://www.readbyqxmd.com/read/28860613/the-stem-cell-regulator-pedf-is-dispensable-for-maintenance-and-function-of-hematopoietic-stem-cells
#2
Emma Rörby, Matilda Billing, Maria Dahl, Sarah Warsi, Silja Andradottir, Kenichi Miharada, Kavitha Siva, Jan-Ingvar Jönsson, Ulrika Blank, Göran Karlsson, Stefan Karlsson
Pigment epithelium derived factor (PEDF), a ubiquitously expressed 50 kDa secreted glycoprotein, was recently discovered to regulate self-renewal of neural stem cells and have a supportive effect on human embryonic stem cell growth. Here, we analyzed expression of PEDF in the murine hematopoietic stem cell (HSC) compartments and found that PEDF is highly expressed in primary long-term HSCs. Therefore, we characterized the hematopoietic system in a knockout mouse model for PEDF and using this model we surprisingly found that PEDF is dispensable for HSC regulation...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28800962/contacting-co-culture-of-human-retinal-microvascular-endothelial-cells-alters-barrier-function-of-human-embryonic-stem-cell-derived-retinal-pigment-epithelial-cells
#3
H Skottman, J Muranen, H Lähdekorpi, E Pajula, K Mäkelä, L Koivusalo, A Koistinen, H Uusitalo, K Kaarniranta, K Juuti-Uusitalo
Here we evaluated the effects of human retinal microvascular endothelial cells (hREC) on mature human embryonic stem cell (hESC) derived retinal pigment epithelial (RPE) cells. The hESC-RPE cells (Regea08/017, Regea08/023 or Regea11/013) and hREC (ACBRI 181) were co-cultured on opposite sides of transparent membranes for up to six weeks. Thereafter barrier function, small molecule permeability, localization of RPE and endothelial cell marker proteins, cellular fine structure, and growth factor secretion of were evaluated...
August 8, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28795779/core-shell-fibrous-scaffold-as-a-vehicle-for-sustained-release-of-retinal-pigmented-epithelium-derived-factor-pedf-for-photoreceptor-differentiation-of-conjunctiva-mesenchymal-stem-cells
#4
Fatemeh Nasehi, Mohsen Karshenas, Samad Nadri, Ghasem Barati, Ahdiye Salim
Coaxial electrospinning technique was introduced as a flexible and promising technique for the fabrication of core-shell fibrous scaffold from poly ethylene glycol/poly caprolactone (PEG/PCL), where retinal pigmented epithelium-derived factor (PEDF) was encapsulated in the core, for photoreceptor differentiation of conjunctiva mesenchymal stem cells (CJMSCs) seed on scaffolds. The morphology and structure of fibers were characterized using SEM and TEM and photoreceptor differentiation was examined by quantitative real time PCR (qPCR)...
August 10, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28771223/pigment-epithelium-derived-factor-promotes-tumor-metastasis-through-an-interaction-with-laminin-receptor-in-hepatocellular-carcinomas
#5
Jianjing Hou, Chao Ge, Meiling Cui, Tengfei Liu, Xiaoqin Liu, Hua Tian, Fangyu Zhao, Taoyang Chen, Ying Cui, Ming Yao, Jinjun Li, Hong Li
Pigment epithelium-derived factor (PEDF) has complex functions in tumor metastasis, but little is known about the roles of PEDF and its receptors in hepatocellular carcinoma (HCC). Here we found that high expression of PEDF is associated with shorter overall survival in HCC patients. Forced expression of PEDF enhanced HCC cell aggressive behavior in vitro and in vivo, whereas silencing PEDF expression reduced migration and invasion. Furthermore, PEDF expression led to changes in cell morphology and the expression of epithelial-mesenchymal transition (EMT)-related markers via ERK1/2 signaling pathway, including the upregulation of N-cadherin and slug, and the downregulation of E-cadherin in HCC cells...
August 3, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28747334/pedf-expression-affects-retinal-endothelial-cell-pro-angiogenic-properties-through-alterations-in-cell-adhesive-mechanisms
#6
Juliana Falero-Perez, SunYoung Park, Christine M Sorenson, Nader Sheibani
Pigment epithelium derived factor (PEDF) is an endogenous inhibitor of angiogenesis. Although various ocular cell types including retinal endothelial cells (EC) produce PEDF, we know very little about cell autonomous effects of PEDF in these cell types. Here we determined how PEDF expression affects retinal EC proangiogenic properties. Retinal EC were prepared from wild type (PEDF+/+) and PEDF-deficient (PEDF-/-) mice. The identity of EC was confirmed by staining for specific markers including VE-cadherin, CD31, and B4-lectin...
July 26, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/28730105/pigment-epithelium-derived-factor-protects-retinal-ganglion-cells-from-hypoxia-induced-apoptosis-by-preventing-mitochondrial-dysfunction
#7
Shu-Wei Tian, Yuan Ren, Jin-Zhi Pei, Bai-Chao Ren, Yuan He
AIM: To investigate the potential of pigment epithelium-derived factor (PEDF) to protect the immortalized rat retinal ganglion cells-5 (RGC-5) exposed to CoCl2-induced chemical hypoxia. METHODS: After being differentiated with staurosporine (SS), RGC-5 cells were cultured in four conditions: control group cells cultured in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal bovine serum, 100 µmol/mL streptomycin and penicillin (named as normal conditions); hypoxia group cells cultured in DMEM containing 300 µmol/mL CoCl2; cells in the group protected by PEDF were first pretreated with 100 ng/mL PEDF for 2h and then cultured in the same condition as hypoxia group cells; and PEDF group cells that were cultured in the presence of 100 ng/mL PEDF under normal conditions...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28728289/-the-protective-effect-of-pigment-epithelial-derived-factor-modified-human-umbilical-cord-mesenchymal-stem-cells-on-rats-with-diabetic-retinopathy
#8
W Zhang, H T Duan, S Chen, Y X Wang, J H Kong, M Dong, X Bi, J Song
Objective: To investigate the effect of pigment epithelial-derived factor (PEDF) gene-modified human umbilical cord mesenchymal stem cells (MSC) on rats with diabetic retinopathy (DR). Methods: Experimental study. Human umbilical cord MSC were transfected by lentivirus packaging PEDF-MSC-green fluorescent protein (GFP) and GFP-MSC plasmid vectors, and the expression of PEDF and vascular endothelial growth factor (VEGF) was measured in the cell culture medium. Fifty adult male Sprague-Dawley rats were randomly divided into five groups: normal control group (group A), DR control group (group B), phosphate-buffered saline (PBS) treated group (group C), GFP-MSC treated group (group D) and PEDF-MSC-GFP treated group (group E), with 10 rats in each group...
July 11, 2017: [Zhonghua Yan Ke za Zhi] Chinese Journal of Ophthalmology
https://www.readbyqxmd.com/read/28706437/comparison-of-two-neurotrophic-serpins-reveals-a-small-fragment-with-cell-survival-activity
#9
Paige N Winokur, Preeti Subramanian, Jeanee L Bullock, Veronique Arocas, S Patricia Becerra
PURPOSE: Protease nexin-1 (PN-1), a serpin encoded by the SERPINE2 gene, has serine protease inhibitory activity and neurotrophic properties in the brain. PN-1 inhibits retinal angiogenesis; however, PN-1's neurotrophic capacities in the retina have not yet been evaluated. Pigment epithelium-derived factor (PEDF) is a serpin that exhibits neurotrophic and antiangiogenic activities but lacks protease inhibitory properties. The aim of this study is to compare PN-1 and PEDF. METHODS: Sequence comparisons were performed using computer bioinformatics programs...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28698158/pigment-epithelium-derived-factor-play-a-positive-role-in-bone-mineralization-of-osteoblasts-derived-from-diabetic-patients
#10
Na Song, Jiateng Zhong, Jinghang Zhang, Jian Yu, Jinsong Li, Jinbo Qi, Jun Yang, Yanyan Qiu, Wei Su, Zhiwei Feng, Haijun Wang
Pigment epithelium-derived factor (PEDF) is a multifunctional secreted protein which plays important role in anti-angiogenic, anti-tumorigenic, as well as involves in the metabolism and regeneration of bone. In this study, our aim is to investigate the role of PEDF in regulating mineralization of osteoblasts from diabetic patients (DP). We isolated and cultured osteoblasts derived from DP and non-diabetic patients (NDP), in order to analyze the variable differences via gene expression and calcification assay in vitro...
September 5, 2017: Gene
https://www.readbyqxmd.com/read/28667418/diabetic-nephropathy-serum-mir-9-confers-a-poor-prognosis-in-and-is-associated-with-level-changes-of-vascular-endothelial-growth-factor-and-pigment-epithelium-derived-factor
#11
Yanxin Xiao, Shuqin Guo, Yunliang Zhang, Zhiying Bian, Liyan Jia, Yanyun Hu, Jie Chen, Chao Yin, Ning Li, Dongxun Zhang, Xincui Zhao, Jun Wang
OBJECTIVE: To investigate the relationship between the serum level of miR-9 and the progression of diabetic nephropathy (DN) and related molecular mechanisms. RESULTS: Thirty-five healthy subjects and 140 DN patients were divided into five groups: control, DN I-II, DN III, DN IV and DN V. Serum level of miR-9 was measured by real-time qPCR. Serum levels of vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF) lipids, fasting glucose, insulin, hemoglobin A1c (HBA1c), creatinine, fibrinogen and insulin resistance (HOMA-IR) were also measured...
June 30, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28666802/a-novel-modified-physiologically-relevant-model-for-cardiac-angiogenesis
#12
Hao Zhang, Jiajun Pan, Fan Qiu, Tengteng Wei, Zhimin Li, Xia Jiang, Wei Zhuang, Zhiwei Liu, Zhongming Zhang, Hongyan Dong
Angiogenesis assays are important tools for studying both the mechanisms of cardiac angiogenesis and the potential development of therapeutic strategies to ischemic heart diseases. Currently, various assays have been used to quantitate cardiac tubule formation, yet no consensus has been reached regarding a suitable assay for evaluating the efficacy of angiogenic stimulants or inhibitors. Most in vivo angiogenesis assays are complex and difficult to interpret, whereas traditional in vitro angiogenesis models measure only one aspect of this process...
June 28, 2017: Microvascular Research
https://www.readbyqxmd.com/read/28662501/cold-exposure-differentially-stimulates-angiogenesis-in-bat-and-wat-of-mice-implication-in-adrenergic-activation
#13
Xiao Luo, Ru Jia, Xiao-Qin Luo, Guan Wang, Qiang-Ling Zhang, Hu Qiao, Nan Wang, Jian-Qun Yan
BACKGROUND/AIMS: To characterize the temporal profile of cold-induced angiogenesis in brown and white adipose tissues of mice in vivo and the temporal changes of angiogenic factors in primary mice brown (BA) and white adipocytes (WA) treated with β3-adrenoceptor agonist (CL316,243) in vitro. METHODS: 8-week old male C57BL/6J mice were individually housed in conventional cages under cold exposure (4°C) for 1, 2, 3, 4 and 5 days. Interscapular brown adipose tissue (iBAT), inguinal subcutaneous (sWAT) and epididymal white adipose tissues (eWAT) were harvested for immunohistochemical and gene expression analysis...
June 27, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28648555/comparison-of-plasma-pigment-epithelium-derived-factor-pedf-retinol-binding-protein-4-rbp-4-chitinase-3-like-protein-1-ykl-40-and-brain-derived-neurotrophic-factor-bdnf-for-the-identification-of-insulin-resistance
#14
F J K Toloza, M C Pérez-Matos, M L Ricardo-Silgado, M C Morales-Álvarez, J O Mantilla-Rivas, J A Pinzón-Cortés, M Pérez-Mayorga, M L Arévalo-García, G Tolosa-González, C O Mendivil
AIMS: To evaluate and compare the association of four potential insulin resistance (IR) biomarkers (pigment-epithelium-derived factor [PEDF], retinol-binding-protein-4 [RBP-4], chitinase-3-like protein 1 [YKL-40] and brain-derived neurotrophic factor [BDNF]) with objective measures of IR. METHODS: We studied 81 subjects with different metabolic profiles. All participants underwent a 5-point OGTT with calculation of multiple IR indexes. A subgroup of 21 participants additionally underwent a hyperinsulinemic-euglycemic clamp...
June 9, 2017: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/28644145/colorectal-carcinoma-with-osseous-metaplasia
#15
Xibo Liu, Jinghong Xu, Lirong Chen
Osseous metaplasia (OM) is rarely observed in colorectal cancer (incidence < 0.4% in rectal cancer), where it has a non-specific clinical presentation and unknown pathogenesis. Here, we report three cases of colorectal carcinoma with OM and propose a new hypothesis. All three patients (two males and one female) were Chinese and had different sites of colorectal carcinoma with OM: rectum, sigmoid colon, and appendix. The pathologic diagnoses were serrated adenocarcinoma; moderately to poorly differentiated adenocarcinoma with micropapillary carcinoma and cribriform comedo-type adenocarcinoma; and mucinous adenocarcinoma, respectively...
June 20, 2017: Oncotarget
https://www.readbyqxmd.com/read/28642110/pedf-plus-dha-modulate-inflammation-and-stimulate-nerve-regeneration-after-hsv-1-infection
#16
Jiucheng He, Donna Neumann, Azucena Kakazu, Thang Luong Pham, Farhana Musarrat, M Soledad Cortina, Haydee E P Bazan
Herpes simplex virus type-1 (HSV-1) infection leads to impaired corneal sensation and, in severe cases, to corneal ulceration, melting and perforation. Here, we explore the potential therapeutic action of pigment epithelial-derived factor (PEDF) plus docosahexaenoic acid (DHA) on corneal inflammation and nerve regeneration following HSV-1 infection. Rabbits inoculated with 100,000 PFU/eye of HSV-1 strain 17Syn+ were treated with PEDF + DHA or vehicle. PEDF + DHA treatment resulted in a biphasic immune response with stronger infiltration of CD4+T cells, neutrophils and macrophages at 7-days post-treatment (p...
August 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28637898/activation-of-complement-by-pigment-epithelium-derived-factor-in-rheumatoid-arthritis
#17
Leonie M Vogt, Simone Talens, Ewa Kwasniewicz, Carsten Scavenius, André Struglics, Jan J Enghild, Tore Saxne, Anna M Blom
The aim of this study was to identify molecules that trigger complement activation in rheumatic joints. C4d, the final cleavage product of C4 activation, is found in the diseased joint and can bind covalently to complement-activating molecules. By using a highly specific Ab against a cleavage neoepitope in C4d, several molecules that were specifically bound to C4d were identified from pooled synovial fluid (SF) from four rheumatoid arthritis (RA) patients. One of these molecules, pigment epithelium-derived factor (PEDF), is a broadly expressed multifunctional member of the serine proteinase inhibitor family...
June 21, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28627623/p18-peptide-a-functional-fragment-of-pigment-epithelial-derived-factor-inhibits-angiogenesis-in-hepatocellular-carcinoma-via-modulating-vegf-vegfr2-signalling-pathway
#18
Xin Wang, Peng Xiu, Fuhai Wang, Jingtao Zhong, Honglong Wei, Zongzhen Xu, Feng Liu, Jie Li
The P18 peptide is a functional fragment of pigment epithelial-derived factor (PEDF), which is an endogenic angiogenesis inhibitor. This study sought to determine the anti-angiogenic bioactivity of the P18 peptide in hepato-cellular carcinoma (HCC) and to elucidate the underlying mechanism. Xenograft tumour growth assays demonstrated the P18 peptide suppressed angiogenesis of HCC in vivo. Wound healing, Transwell and Matrigel-culture assays indicated that the P18 peptide inhibited the cell migration and tube formation of endothelial cells (ECs) in vitro...
June 14, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28620131/exosomes-containing-differential-expression-of-microrna-and-mrna-in-osteosarcoma-that-can-predict-response-to-chemotherapy
#19
Ji-Feng Xu, Ya-Ping Wang, Shui-Jun Zhang, Yu Chen, Hai-Feng Gu, Xiao-Fan Dou, Bing Xia, Qing Bi, Shun-Wu Fan
A major challenge in osteosarcoma (OS) is the selection of the most effective chemotherapeutic agents for individual patients, while the administration of ineffective chemotherapy increases mortality and decreases quality of life in patients. This emphasizes the need to evaluate every patient's probability of responding to each chemotherapeutic agent. We developed a profiling strategy for serum exosomal microRNAs and mRNAs in OS patients with differential chemotherapeutic responses. Twelve miRNAs were up regulated and 18 miRNAs were under regulated significantly in OS patient with poor chemotherapeutic response when compared with those in good chemotherapeutic response (p<0...
June 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28602715/microstructure-dependent-binding-of-pigment-epithelium-derived-factor-pedf-to-type-i-collagen-fibrils
#20
Meagan Cauble, Phillip Yang, Ulrich Baumann, Jan M Gebauer, Bradford G Orr, Le T Duong, Mark M Banaszak Holl
Pigment epithelium derived factor (PEDF) is a multifunctional extracellular protein. In addition to its known anti-angiogenic and neurotrophic roles in collagen rich tissues, PEDF is thought to be involved in collagen fibril assembly due to its sequence specific binding to the collagen fibril and high expression in regions of active bone formation. In order to image the presence of the protein on the fibrils, PEDF was recombinantly made with a strep tag (strep-PEDF) and then gold nanoparticles conjugated to streptavidin (AuNP) were used as a secondary tag...
June 12, 2017: Journal of Structural Biology
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