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https://www.readbyqxmd.com/read/28053185/macrophage-ltb4-drives-efficient-phagocytosis-of-borrelia-burgdorferi-via-blt1-or-blt2
#1
Yan Zhang, Rachel M Olson, Charles R Brown
Unresolved experimental Lyme arthritis in C3H 5-LOX-/- mice is associated with impaired macrophage phag-ocytosis of Borrelia burgdorferi. In the present study, we further investigated the effects of the 5-LOX metabolite, LTB4, on phagocytosis of B. burgdorferi. Bone marrow-derived macrophages (BMDM) from 5-LOX-/- mice were defective in the uptake and killing of B. burgdorferi from the earliest stages of spirochete internali-zation. BMDM from mice deficient for the LTB4 high-affinity receptor (BLT1-/-) were also unable to efficiently phagocytose B...
January 4, 2017: Journal of Lipid Research
https://www.readbyqxmd.com/read/28032600/the-function-of-cancer-shed-gangliosides-in-macrophage-phenotype-involvement-with-angiogenesis
#2
Tae-Wook Chung, Hee-Jung Choi, Mi-Ju Park, Hee-Jin Choi, Syng-Ook Lee, Keuk-Jun Kim, Cheorl-Ho Kim, Changwan Hong, Kyun-Ha Kim, Myungsoo Joo, Ki-Tae Ha
Tumor-derived gangliosides in the tumor microenvironment are involved in the malignant progression of cancer. However, the molecular mechanisms underlying the effects of gangliosides shed from tumors on macrophage phenotype remain unknown. Here, we showed that ganglioside GM1 highly induced the activity and expression of arginase-1 (Arg-1), a major M2 macrophage marker, compared to various gangliosides in bone marrow-derived macrophages (BMDM), peritoneal macrophages and Raw264.7 macrophage cells. We found that GM1 bound to macrophage mannose receptor (MMR/CD206) and common gamma chain (γc)...
December 10, 2016: Oncotarget
https://www.readbyqxmd.com/read/28001907/ionizing-radiation-induces-innate-immune-responses-in-macrophages-by-generation-of-mitochondrial-reactive-oxygen-species
#3
Sup Kim, Jin Ho Choe, Gippeum Joy Lee, Yi Sak Kim, Soo Yeon Kim, Hye-Mi Lee, Hyo Sun Jin, Tae Sung Kim, Jin-Man Kim, Moon-June Cho, Eui-Cheol Shin, Eun-Kyeong Jo, Jun-Sang Kim
During radiotherapy for tumors, the innate immune system also responds to ionizing radiation and induces immune modulation. However, little is known about the molecular mechanisms by which radiation modulates innate immune responses. In this study, we observed that radiation triggered the generation of mitochondrial reactive oxygen species (mROS), leading to innate immune responses in murine bone marrow-derived macrophages (BMDM). Radiation-induced mROS was essential for robust induction of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-12p40 mRNA and protein in BMDM...
December 21, 2016: Radiation Research
https://www.readbyqxmd.com/read/27987189/immunomodulatory-properties-of-filamentous-fungi-cultivated-through-solid-state-fermentation-on-rapeseed-meal
#4
Stéphanie Sutter, France Thevenieau, Anne Bourdillon, Joëlle De Coninck
Water extracts from solid-state fermentation (SSF) on rapeseed meal using filamentous fungi exhibit interesting immunomodulatory activities in vitro. Immunomodulation was determined by the capacity of the compounds to activate blood neutrophils and to influence cytokine production in human peripheral blood mononuclear cells (PBMC) and mouse bone marrow-derived macrophages (BMDM). Among the strains tested, Aspergillus sojae mycelium and SSF extracts were the most promising in terms of enhancing the immune response...
December 16, 2016: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27974684/macrophage-production-and-activation-are-dependent-on-trim33
#5
Anne-Sophie Gallouet, Federica Ferri, Vanessa Petit, Aude Parcelier, Daniel Lewandowski, Nathalie Gault, Vilma Barroca, Stéphanie Le Gras, Eric Soler, Frank Grosveld, Irwin Davidson, Paul-Henri Romeo
The tripartite motif (TRIM) family of proteins plays important roles in innate immunity and antimicrobial infection. None of these proteins has been shown to directly regulate transcription of genes in monocyte/macrophage except TRIM33 that we have recently shown to be a macrophage specific transcriptional inhibitor of Ifnb1. Using ChIP-seq analyses, we now report that TRIM33 is bound to two fold more genes in immature than in mature myeloid cell lines. When located near the same genes, TRIM33 is bound to different sequences in the two cell lines suggesting a role of TRIM33 in both immature and mature myeloid cells...
December 10, 2016: Oncotarget
https://www.readbyqxmd.com/read/27933275/an-in-vitro-co-culture-mouse-model-demonstrates-efficient-vaccine-mediated-control-of-francisella-tularensis-schu-s4-and-identifies-nitric-oxide-as-a-predictor-of-efficacy
#6
Igor Golovliov, Helena Lindgren, Kjell Eneslätt, Wayne Conlan, Amandine Mosnier, Thomas Henry, Anders Sjöstedt
Francisella tularensis is a highly virulent intracellular bacterium and cell-mediated immunity is critical for protection, but mechanisms of protection against highly virulent variants, such as the prototypic strain F. tularensis strain SCHU S4, are poorly understood. To this end, we established a co-culture system, based on splenocytes from naïve, or immunized mice and in vitro infected bone marrow-derived macrophages that allowed assessment of mechanisms controlling infection with F. tularensis. We utilized the system to understand why the clpB gene deletion mutant, ΔclpB, of SCHU S4 shows superior efficacy as a vaccine in the mouse model as compared to the existing human vaccine, the live vaccine strain (LVS)...
2016: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/27898698/myeloid-dll4-does-not-contribute-to-the-pathogenesis-of-non-alcoholic-steatohepatitis-in-ldlr-mice
#7
Mike L J Jeurissen, Sofie M A Walenbergh, Tom Houben, Tim Hendrikx, Jieyi Li, Yvonne Oligschlaeger, Patrick J van Gorp, Marion J J Gijbels, Albert Bitorina, Isabell Nessel, Freddy Radtke, Marc Vooijs, Jan Theys, Ronit Shiri-Sverdlov
Non-alcoholic steatohepatitis (NASH) is characterized by liver steatosis and inflammation. Currently, the underlying mechanisms leading to hepatic inflammation are not fully understood and consequently, therapeutic options are poor. Non-alcoholic steatohepatitis (NASH) and atherosclerosis share the same etiology whereby macrophages play a key role in disease progression. Macrophage function can be modulated via activation of receptor-ligand binding of Notch signaling. Relevantly, global inhibition of Notch ligand Delta-Like Ligand-4 (DLL4) attenuates atherosclerosis by altering the macrophage-mediated inflammatory response...
2016: PloS One
https://www.readbyqxmd.com/read/27866511/impaired-phagocytosis-of-apoptotic-cells-causes-accumulation-of-bone-marrow-derived-macrophages-in-aged-mice
#8
Ok-Hee Kim, Hyojung Kim, Jinku Kang, Dongki Yang, Yu-Hoi Kang, Dae Ho Lee, Gi Jeong Cheon, Sang Chul Park, Byung-Chul Oh
Accumulation of tissue macrophages is a significant characteristic of disease-associated chronic inflammation, and facilitates the progression of disease pathology. However, the functional roles of these bone marrow-derived macrophages (BMDMs) in aging are unclear. Here, we identified age-dependent macrophage accumulation in the bone marrow, showing that aging significantly increases the number of M1 macrophages and impairs polarization of BMDMs. We found that age-related dysregulation of BMDMs is associated with abnormal overexpression of the anti-inflammatory interleukin-10...
November 18, 2016: BMB Reports
https://www.readbyqxmd.com/read/27818939/osteopontin-is-a-key-player-for-local-adipose-tissue-macrophage-proliferation-in-obesity
#9
Matteo Tardelli, Karina Zeyda, Veronica Moreno-Viedma, Bettina Wanko, Nicole G Grün, Günther Staffler, Maximilian Zeyda, Thomas M Stulnig
OBJECTIVE: Recent findings point towards an important role of local macrophage proliferation also in obesity-induced adipose tissue inflammation that underlies insulin resistance and type 2 diabetes. Osteopontin (OPN) is an inflammatory cytokine highly upregulated in adipose tissue (AT) of obese and has repeatedly been shown to be functionally involved in adipose-tissue inflammation and metabolic sequelae. In the present work, we aimed at unveiling both the role of OPN in human monocyte and macrophage proliferation as well as the impact of OPN deficiency on local macrophage proliferation in a mouse model for diet-induced obesity...
November 2016: Molecular Metabolism
https://www.readbyqxmd.com/read/27795296/phd2-is-a-regulator-for-glycolytic-reprogramming-in-macrophages
#10
Annemarie Guentsch, Angelika Beneke, Lija Swain, Katja Farhat, Shunmugam Nagarajan, Ben Wielockx, Kaamini Raithatha, Jan Dudek, Peter Rehling, Anke Zieseniss, Aline Jatho, Mei Chong, Celio X C Santos, Ajay M Shah, Dörthe M Katschinski
The prolyl-4-hydroxylase domain (PHD) enzymes are regarded as the molecular oxygen sensors. There is an interplay between oxygen availability and cellular metabolism, which in turn has significant effects on the functionality of innate immune cells, such as macrophages. However, if and how PHD enzymes affect macrophage metabolism are enigmatic. We hypothesized that macrophage metabolism and function can be controlled via manipulation of PHD2. We characterized the metabolic phenotypes of PHD2-deficient RAW cells and primary PHD2 knockout bone marrow-derived macrophages (BMDM)...
January 1, 2017: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27793708/cholesteryl-hemiesters-alter-lysosome-structure-and-function-and-induce-proinflammatory-cytokine-production-in-macrophages
#11
Neuza Domingues, Luís M B B Estronca, João Silva, Marisa R Encarnação, Rita Mateus, Diogo Silva, Inês B Santarino, Margarida Saraiva, Maria I L Soares, Teresa M V D Pinho E Melo, António Jacinto, Winchil L C Vaz, Otília V Vieira
RATIONALE: Cholesteryl hemiesters are oxidation products of polyunsaturated fatty acid esters of cholesterol. Their oxo-ester precursors have been identified as important components of the "core aldehydes" of human atheromata and in oxidized lipoproteins (Ox-LDL). We had previously shown, for the first time, that a single compound of this family, cholesteryl hemisuccinate (ChS), is sufficient to cause irreversible lysosomal lipid accumulation (lipidosis), and is toxic to macrophages. These features, coupled to others such as inflammation, are typically seen in atherosclerosis...
February 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27785087/adiponectin-treatment-attenuates-inflammatory-response-during-early-sepsis-in-obese-mice
#12
XianFeng Wang, Nancy L Buechler, Barbara K Yoza, Charles E McCall, Vidula Vachharajani
BACKGROUND: Morbid obesity increases the cost of care in critically ill patients. Sepsis is the leading cause of death in noncoronary intensive care units. Circulating cell-endothelial cell interactions in microcirculation are the rate-determining factors in any inflammation; obesity increases these interactions further. Adiponectin deficiency is implicated in increased cardiovascular risk in obese patients. We have shown that adiponectin deficiency increases microvascular dysfunction in early sepsis...
2016: Journal of Inflammation Research
https://www.readbyqxmd.com/read/27727129/recombinant-bacillus-subtilis-spores-for-the-delivery-of-mycobacterium-tuberculosis-ag85b-cfp10-secretory-antigens
#13
Kishore Das, Tima Thomas, Omar Garnica, Subramanian Dhandayuthapani
Tuberculosis continues to be a great cause of morbidity and mortality in different parts of the world. Unfortunately, the current BCG vaccine being administered is not fully protective against tuberculosis; therefore, there is a great need for alternate vaccines. With an aim to develop such vaccines, we have analyzed the utility of Bacillus subtilis spores for the expression of two major immunodominant antigens of Mycobacterium tuberculosis, Ag85B and CFP10. We created three recombinant B. subtilis strains to express a truncated fusion of Ag85B191-325 and CFP101-70 antigens (T85BCFP), either on the spore coat (MTAG1 strain) or in the cytosol of B...
December 2016: Tuberculosis
https://www.readbyqxmd.com/read/27720677/phagocytosis-of-bone-marrow-derived-macrophages-is-controlled-by-phosphoinositide-3-kinase-%C3%AE
#14
Anne Kresinsky, Nadine Schneble, Caroline Schmidt, Adrian Frister, Reinhard Bauer, Reinhard Wetzker, Jörg P Müller
Due to their ability to phagocytise invading microbes macrophages play a key role in the innate and acquired immune system. In this article the role of phosphoinositide 3-kinase gamma (PI3Kγ) for phagocytosis was studied in bone marrow derived macrophages (BMDM). By using genetic and pharmacological approaches our data clearly demonstrate PI3Kγ is acting as a mediator of macrophage phagocytosis. Phagocytosis of LPS activated BMDM was reduced in PI3Kγ depleted primary BMDM or macrophage cell line J774. Depletion of other class I phosphoinositide 3-kinases did not alter phagocytic activity...
October 5, 2016: Immunology Letters
https://www.readbyqxmd.com/read/27692982/mtorc1-inhibition-with-rapamycin-exacerbates-adipose-tissue-inflammation-in-obese-mice-and-dissociates-macrophage-phenotype-from-function
#15
Vivian A Paschoal, Mariane T Amano, Thiago Belchior, Juliana Magdalon, Patricia Chimin, Maynara L Andrade, Milene Ortiz-Silva, Érique Castro, Alex S Yamashita, José Cesar Rosa Neto, Niels O Câmara, William T Festuccia
Genetic- and diet-induced obesity and insulin resistance are associated with an increase in mechanistic target of rapamycin complex (mTORC) 1 activity in adipose tissue. We investigated herein the effects of pharmacological mTORC1 inhibition in the development of adipose tissue inflammation induced by high-fat diet (HFD) feeding, as well as in the polarization, metabolism and function of bone marrow-derived macrophages (BMDM). For this, C57BL/6J mice fed with a standard chow diet or a HFD (60% of calories from fat) and treated with either vehicle (0...
February 2017: Immunobiology
https://www.readbyqxmd.com/read/27662364/leishmania-donovani-inhibits-macrophage-apoptosis-and-pro-inflammatory-response-through-akt-mediated-regulation-of-%C3%AE-catenin-and-foxo-1
#16
Purnima Gupta, Supriya Srivastav, Shriya Saha, Pijush K Das, Anindita Ukil
In order to establish infection, intra-macrophage parasite Leishmania donovani needs to inhibit host defense parameters like inflammatory cytokine production and apoptosis. In the present study, we demonstrate that the parasite achieves both by exploiting a single host regulator AKT for modulating its downstream transcription factors, β-catenin and FOXO-1. L. donovani-infected RAW264.7 and bone marrow-derived macrophages (BMDM) treated with AKT inhibitor or dominant negative AKT constructs showed decreased anti-inflammatory cytokine production and increased host cell apoptosis resulting in reduced parasite survival...
November 1, 2016: Cell Death and Differentiation
https://www.readbyqxmd.com/read/27641912/atp-induced-il-1%C3%AE-secretion-is-selectively-impaired-in-microglia-as-compared-to-hematopoietic-macrophages
#17
Saskia Maria Burm, Ella Alwine Zuiderwijk-Sick, Paola Massiel Weert, Jeffrey John Bajramovic
Under stressful conditions nucleotides are released from dying cells into the extracellular space, where they can bind to purinergic P2X and P2Y receptors. High concentrations of extracellular ATP in particular induce P2X7-mediated signaling, which leads to inflammasome activation. This in turn leads to the processing and secretion of pro-inflammatory cytokines, like interleukin (IL)-1β. During neurodegenerative diseases, innate immune responses are shaped by microglia and we have previously identified microglia-specific features of inflammasome-mediated responses...
September 19, 2016: Glia
https://www.readbyqxmd.com/read/27632706/inhibition-of-lanthanide-nanocrystal-induced-inflammasome-activation-in-macrophages-by-a-surface-coating-peptide-through-abrogation-of-ros-production-and-trpm2-mediated-ca-2-influx
#18
Han Yao, Yunjiao Zhang, Liu Liu, Youcui Xu, Xi Liu, Jun Lin, Wei Zhou, Pengfei Wei, Peipei Jin, Long-Ping Wen
Lanthanide-based nanoparticles (LNs) hold great promise in medicine. A variety of nanocrystals, including LNs, elicits potent inflammatory response through activation of NLRP3 inflammasome. We have previously identified an LNs-specific surface coating peptide RE-1, with the sequence of 'ACTARSPWICG', which reduced nanocrystal-cell interaction and abrogated LNs-induced autophagy and toxicity in both HeLa cells and liver hepatocytes. Here we show that RE-1 coating effectively inhibited LNs-induced inflammasome activation, mostly mediated by NLRP3, in mouse bone marrow derived macrophage (BMDM) cells, human THP-1 cells and mouse peritoneal macrophages and also reduced LNs-elicited inflammatory response in vivo...
November 2016: Biomaterials
https://www.readbyqxmd.com/read/27597295/plasma-membrane-bound-g-protein-coupled-bile-acid-receptor-attenuates-liver-ischemia-reperfusion-injury-via-the-inhibition-of-toll-like-receptor-4-signaling-in-mice
#19
Haojun Yang, Haoming Zhou, Lin Zhuang, Johan Auwerx, Kristina Schoonjans, Xuehao Wang, Cheng Feng, Ling Lu
The plasma membrane-bound G protein-coupled bile acid receptor (TGR5) displays varied levels of expression in different tissues. TGR5-induced liver protection has been demonstrated during several liver diseases, except during ischemia/reperfusion injury (IRI). Male adult wild-type and TGR5 knockout (KO) mice were subjected to liver partial warm ischemia/reperfusion. Hepatic injury was evaluated based on serum alanine aminotransferase and serum aspartate aminotransferase. Liver histological injury and inflammatory cell infiltration were evaluated in tissue sections using liver immunohistochemical analysis...
January 2017: Liver Transplantation
https://www.readbyqxmd.com/read/27590510/ll202-protects-against-dextran-sulfate-sodium-induced-experimental-colitis-in-mice-by-inhibiting-mapk-ap-1-signaling
#20
Yuan Gao, Yujie Huang, Yue Zhao, Yang Hu, Zhiyu Li, Qinglong Guo, Kai Zhao, Na Lu
LL202, a newly-synthesized flavonoid derivative, has been reported to inhibit inflammatory-induced angiogenesis. However, the exact role of LL202 in inflammation along with its mechanism has not been explored. In this study, we investigated the anti-inflammatory effect of LL202 on intestinal inflammation by establishing dextran sulfate sodium (DSS)-induced experimental colitis. LL202 attenuated DSS-induced body weight loss, colon length shortening and colonic pathological damage. The inflammatory cells infiltration, myeloperoxidase (MPO) and inducible nitric oxide synthase (iNOS) activities were decreased by LL202 in a dose-dependent manner...
September 27, 2016: Oncotarget
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