keyword
MENU ▼
Read by QxMD icon Read
search

BMDM

keyword
https://www.readbyqxmd.com/read/27898698/myeloid-dll4-does-not-contribute-to-the-pathogenesis-of-non-alcoholic-steatohepatitis-in-ldlr-mice
#1
Mike L J Jeurissen, Sofie M A Walenbergh, Tom Houben, Tim Hendrikx, Jieyi Li, Yvonne Oligschlaeger, Patrick J van Gorp, Marion J J Gijbels, Albert Bitorina, Isabell Nessel, Freddy Radtke, Marc Vooijs, Jan Theys, Ronit Shiri-Sverdlov
Non-alcoholic steatohepatitis (NASH) is characterized by liver steatosis and inflammation. Currently, the underlying mechanisms leading to hepatic inflammation are not fully understood and consequently, therapeutic options are poor. Non-alcoholic steatohepatitis (NASH) and atherosclerosis share the same etiology whereby macrophages play a key role in disease progression. Macrophage function can be modulated via activation of receptor-ligand binding of Notch signaling. Relevantly, global inhibition of Notch ligand Delta-Like Ligand-4 (DLL4) attenuates atherosclerosis by altering the macrophage-mediated inflammatory response...
2016: PloS One
https://www.readbyqxmd.com/read/27866511/impaired-phagocytosis-of-apoptotic-cells-causes-accumulation-of-bone-marrow-derived-macrophages-in-aged-mice
#2
Ok-Hee Kim, Hyojung Kim, Jinku Kang, Dongki Yang, Yu-Hoi Kang, Dae Ho Lee, Gi Jeong Cheon, Sang Chul Park, Byung-Chul Oh
Accumulation of tissue macrophages is a significant characteristic of disease-associated chronic inflammation, and facilitates the progression of disease pathology. However, the functional roles of these bone marrow-derived macrophages (BMDMs) in aging are unclear. Here, we identified age-dependent macrophage accumulation in the bone marrow, showing that aging significantly increases the number of M1 macrophages and impairs polarization of BMDMs. We found that age-related dysregulation of BMDMs is associated with abnormal overexpression of the anti-inflammatory interleukin-10...
November 18, 2016: BMB Reports
https://www.readbyqxmd.com/read/27818939/osteopontin-is-a-key-player-for-local-adipose-tissue-macrophage-proliferation-in-obesity
#3
Matteo Tardelli, Karina Zeyda, Veronica Moreno-Viedma, Bettina Wanko, Nicole G Grün, Günther Staffler, Maximilian Zeyda, Thomas M Stulnig
OBJECTIVE: Recent findings point towards an important role of local macrophage proliferation also in obesity-induced adipose tissue inflammation that underlies insulin resistance and type 2 diabetes. Osteopontin (OPN) is an inflammatory cytokine highly upregulated in adipose tissue (AT) of obese and has repeatedly been shown to be functionally involved in adipose-tissue inflammation and metabolic sequelae. In the present work, we aimed at unveiling both the role of OPN in human monocyte and macrophage proliferation as well as the impact of OPN deficiency on local macrophage proliferation in a mouse model for diet-induced obesity...
November 2016: Molecular Metabolism
https://www.readbyqxmd.com/read/27795296/phd2-is-a-regulator-for-glycolytic-reprogramming-in-macrophages
#4
Annemarie Guentsch, Angelika Beneke, Lija Swain, Katja Farhat, Shunmugam Nagarajan, Ben Wielockx, Kaamini Raithatha, Jan Dudek, Peter Rehling, Anke Zieseniss, Aline Jatho, Mei Chong, Celio X C Santos, Ajay M Shah, Dörthe M Katschinski
The prolyl-4-hydroxylase domain (PHD) enzymes are regarded as the molecular oxygen sensors. There is an interplay between oxygen availability and cellular metabolism, which in turn has significant effects on the functionality of innate immune cells like macrophages. If and how PHDs affect macrophage metabolism however is enigmatic. We hypothesized that via manipulation of PHD2 macrophage metabolism and function can be controlled. We characterized the metabolic phenotype of PHD2-deficient RAW cells and primary PHD2 knock out bone marrow derived macrophages (BMDM)...
October 17, 2016: Molecular and Cellular Biology
https://www.readbyqxmd.com/read/27793708/cholesteryl-hemiesters-alter-lysosome-structure-and-function-and-induce-proinflammatory-cytokine-production-in-macrophages
#5
Neuza Domingues, Luís M B B Estronca, João Silva, Marisa R Encarnação, Rita Mateus, Diogo Silva, Inês B Santarino, Margarida Saraiva, Maria I L Soares, Teresa M V D Pinho E Melo, António Jacinto, Winchil L C Vaz, Otília V Vieira
RATIONALE: Cholesteryl hemiesters are oxidation products of polyunsaturated fatty acid esters of cholesterol. Their oxo-ester precursors have been identified as important components of the "core aldehydes" of human atheromata and in oxidized lipoproteins (Ox-LDL). We had previously shown, for the first time, that a single compound of this family, cholesteryl hemisuccinate (ChS), is sufficient to cause irreversible lysosomal lipid accumulation (lipidosis), and is toxic to macrophages. These features, coupled to others such as inflammation, are typically seen in atherosclerosis...
October 26, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27785087/adiponectin-treatment-attenuates-inflammatory-response-during-early-sepsis-in-obese-mice
#6
XianFeng Wang, Nancy L Buechler, Barbara K Yoza, Charles E McCall, Vidula Vachharajani
BACKGROUND: Morbid obesity increases the cost of care in critically ill patients. Sepsis is the leading cause of death in noncoronary intensive care units. Circulating cell-endothelial cell interactions in microcirculation are the rate-determining factors in any inflammation; obesity increases these interactions further. Adiponectin deficiency is implicated in increased cardiovascular risk in obese patients. We have shown that adiponectin deficiency increases microvascular dysfunction in early sepsis...
2016: Journal of Inflammation Research
https://www.readbyqxmd.com/read/27727129/recombinant-bacillus-subtilis-spores-for-the-delivery-of-mycobacterium-tuberculosis-ag85b-cfp10-secretory-antigens
#7
Kishore Das, Tima Thomas, Omar Garnica, Subramanian Dhandayuthapani
Tuberculosis continues to be a great cause of morbidity and mortality in different parts of the world. Unfortunately, the current BCG vaccine being administered is not fully protective against tuberculosis; therefore, there is a great need for alternate vaccines. With an aim to develop such vaccines, we have analyzed the utility of Bacillus subtilis spores for the expression of two major immunodominant antigens of Mycobacterium tuberculosis, Ag85B and CFP10. We created three recombinant B. subtilis strains to express a truncated fusion of Ag85B191-325 and CFP101-70 antigens (T85BCFP), either on the spore coat (MTAG1 strain) or in the cytosol of B...
September 28, 2016: Tuberculosis
https://www.readbyqxmd.com/read/27720677/phagocytosis-of-bone-marrow-derived-macrophages-is-controlled-by-phosphoinositide-3-kinase-%C3%AE
#8
Anne Kresinsky, Nadine Schneble, Caroline Schmidt, Adrian Frister, Reinhard Bauer, Reinhard Wetzker, Jörg P Müller
Due to their ability to phagocytise invading microbes macrophages play a key role in the innate and acquired immune system. In this article the role of phosphoinositide 3-kinase gamma (PI3Kγ) for phagocytosis was studied in bone marrow derived macrophages (BMDM). By using genetic and pharmacological approaches our data clearly demonstrate PI3Kγ is acting as a mediator of macrophage phagocytosis. Phagocytosis of LPS activated BMDM was reduced in PI3Kγ depleted primary BMDM or macrophage cell line J774. Depletion of other class I phosphoinositide 3-kinases did not alter phagocytic activity...
October 5, 2016: Immunology Letters
https://www.readbyqxmd.com/read/27692982/mtorc1-inhibition-with-rapamycin-exacerbates-adipose-tissue-inflammation-in-obese-mice-and-dissociates-macrophage-phenotype-from-function
#9
Vivian A Paschoal, Mariane T Amano, Thiago Belchior, Juliana Magdalon, Patricia Chimin, Maynara L Andrade, Milene Ortiz-Silva, Érique Castro, Alex S Yamashita, José Cesar Rosa Neto, Niels O Câmara, William T Festuccia
Genetic- and diet-induced obesity and insulin resistance are associated with an increase in mechanistic target of rapamycin complex (mTORC) 1 activity in adipose tissue. We investigated herein the effects of pharmacological mTORC1 inhibition in the development of adipose tissue inflammation induced by high-fat diet (HFD) feeding, as well as in the polarization, metabolism and function of bone marrow-derived macrophages (BMDM). For this, C57BL/6J mice fed with a standard chow diet or a HFD (60% of calories from fat) and treated with either vehicle (0...
September 28, 2016: Immunobiology
https://www.readbyqxmd.com/read/27662364/leishmania-donovani-inhibits-macrophage-apoptosis-and-pro-inflammatory-response-through-akt-mediated-regulation-of-%C3%AE-catenin-and-foxo-1
#10
Purnima Gupta, Supriya Srivastav, Shriya Saha, Pijush K Das, Anindita Ukil
In order to establish infection, intra-macrophage parasite Leishmania donovani needs to inhibit host defense parameters like inflammatory cytokine production and apoptosis. In the present study, we demonstrate that the parasite achieves both by exploiting a single host regulator AKT for modulating its downstream transcription factors, β-catenin and FOXO-1. L. donovani-infected RAW264.7 and bone marrow-derived macrophages (BMDM) treated with AKT inhibitor or dominant negative AKT constructs showed decreased anti-inflammatory cytokine production and increased host cell apoptosis resulting in reduced parasite survival...
November 1, 2016: Cell Death and Differentiation
https://www.readbyqxmd.com/read/27641912/atp-induced-il-1%C3%AE-secretion-is-selectively-impaired-in-microglia-as-compared-to-hematopoietic-macrophages
#11
Saskia Maria Burm, Ella Alwine Zuiderwijk-Sick, Paola Massiel Weert, Jeffrey John Bajramovic
Under stressful conditions nucleotides are released from dying cells into the extracellular space, where they can bind to purinergic P2X and P2Y receptors. High concentrations of extracellular ATP in particular induce P2X7-mediated signaling, which leads to inflammasome activation. This in turn leads to the processing and secretion of pro-inflammatory cytokines, like interleukin (IL)-1β. During neurodegenerative diseases, innate immune responses are shaped by microglia and we have previously identified microglia-specific features of inflammasome-mediated responses...
September 19, 2016: Glia
https://www.readbyqxmd.com/read/27632706/inhibition-of-lanthanide-nanocrystal-induced-inflammasome-activation-in-macrophages-by-a-surface-coating-peptide-through-abrogation-of-ros-production-and-trpm2-mediated-ca-2-influx
#12
Han Yao, Yunjiao Zhang, Liu Liu, Youcui Xu, Xi Liu, Jun Lin, Wei Zhou, Pengfei Wei, Peipei Jin, Long-Ping Wen
Lanthanide-based nanoparticles (LNs) hold great promise in medicine. A variety of nanocrystals, including LNs, elicits potent inflammatory response through activation of NLRP3 inflammasome. We have previously identified an LNs-specific surface coating peptide RE-1, with the sequence of 'ACTARSPWICG', which reduced nanocrystal-cell interaction and abrogated LNs-induced autophagy and toxicity in both HeLa cells and liver hepatocytes. Here we show that RE-1 coating effectively inhibited LNs-induced inflammasome activation, mostly mediated by NLRP3, in mouse bone marrow derived macrophage (BMDM) cells, human THP-1 cells and mouse peritoneal macrophages and also reduced LNs-elicited inflammatory response in vivo...
November 2016: Biomaterials
https://www.readbyqxmd.com/read/27597295/gpbar1-tgr5-attenuates-liver-ischemia-reperfusion-injury-via-the-inhibition-of-tlr4-signaling-in-mice
#13
Haojun Yang, Haoming Zhou, Lin Zhuang, Xuehao Wang, Ling Lv
BACKGROUND: The G protein-coupled receptor TGR5 is a novel plasma membrane-bound G protein-coupled bile acid (BA) receptor. TGR5-induced liver protection has been demonstrated during several liver diseases, except during ischemia/reperfusion injury (IRI). METHOD: Male adult wild-type and TGR5 knockout mice were subjected to liver partial warm ischemia/reperfusion. Hepatic injury was evaluated based on serum alanine aminotransferase (ALT) and serum aspartate aminotransferase (AST)...
September 6, 2016: Liver Transplantation
https://www.readbyqxmd.com/read/27590510/ll202-protects-against-dextran-sulfate-sodium-induced-experimental-colitis-in-mice-by-inhibiting-mapk-ap-1-signaling
#14
Yuan Gao, Yujie Huang, Yue Zhao, Yang Hu, Zhiyu Li, Qinglong Guo, Kai Zhao, Na Lu
LL202, a newly-synthesized flavonoid derivative, has been reported to inhibit inflammatory-induced angiogenesis. However, the exact role of LL202 in inflammation along with its mechanism has not been explored. In this study, we investigated the anti-inflammatory effect of LL202 on intestinal inflammation by establishing dextran sulfate sodium (DSS)-induced experimental colitis. LL202 attenuated DSS-induced body weight loss, colon length shortening and colonic pathological damage. The inflammatory cells infiltration, myeloperoxidase (MPO) and inducible nitric oxide synthase (iNOS) activities were decreased by LL202 in a dose-dependent manner...
August 31, 2016: Oncotarget
https://www.readbyqxmd.com/read/27570978/polarized-macrophages-treated-with-nonylphenol-differently-regulate-lipopolysaccharide-induced-sepsis
#15
Jae-Wook Lee, Sojin Park, Hye-Kyoung Han, Sung Hee Um, Eun-Yi Moon
Nonylphenol (NP) as well-known "endocrine disrupter" influences sexual and reproductive development. Here, we investigated the effect of NP on M1-/M2-type macrophages and their role in lipopolysaccharide (LPS)-induced sepsis. Polarized macrophages of M1- and M2-types were obtained by the treatment with LPS and interleukin-4 (IL-4) to bone marrow-derived macrophages (BMDM), respectively. Coincubation of M1-macrophages with NP decreased COX-2, iNOS, IL-6, and TNF-α expression but no changes were detected in the production of nitric oxide (NO)...
August 29, 2016: Environmental Toxicology
https://www.readbyqxmd.com/read/27566204/paeoniflorin-inhibits-high-glucose-induced-macrophage-activation-through-tlr2-dependent-signal-pathways
#16
Yun-Xia Shao, Xin-Xing Xu, Yuan-Yuan Li, Xiang-Ming Qi, Kun Wang, Yong-Gui Wu, Xiao-Ming Meng
ETHNOPHARMACOLOGICAL RELEVANCE: Paeoniflorin(PF), extracted from the root peeled of Paeonia lactiflora Pall(Family: Ranunculaceae), has therapeutic potential in many animal models of inflammatory diseases. AIM OF THE STUDY: Although the anti-inflammatory efficacy of PF has been well illustrated in several animal models, whether it could attenuate diabetic nephropathy and detailed mechanisms are still obscure. Till now, accumulating evidence has proposed the pivotal role of toll-like receptors (TLRs) in renal inflammation in diabetic patients...
August 23, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27509208/netrin-1-reduces-monocyte-and-macrophage-chemotaxis-towards-the-complement-component-c5a
#17
Lewis Taylor, Maximillian Hugo Brodermann, David McCaffary, Asif Jilani Iqbal, David R Greaves
Netrin-1, acting at its cognate receptor UNC5b, has been previously demonstrated to inhibit CC chemokine-induced immune cell migration. In line with this, we found that netrin-1 was able to inhibit CCL2-induced migration of bone marrow derived macrophages (BMDMs). However, whether netrin-1 is capable of inhibiting chemotaxis to a broader range of chemoattractants remains largely unexplored. As our initial experiments demonstrated that RAW264.7 and BMDMs expressed high levels of C5a receptor 1 (C5aR1) on their surface, we aimed to determine the effect of netrin-1 exposure on monocyte/macrophage cell migration induced by C5a, a complement peptide that plays a major role in multiple inflammatory pathologies...
2016: PloS One
https://www.readbyqxmd.com/read/27475716/a-novel-real-time-imaging-platform-to-quantify-macrophage-phagocytosis
#18
Theodore S Kapellos, Lewis Taylor, Heyne Lee, Sally A Cowley, William S James, Asif J Iqbal, David R Greaves
Phagocytosis of pathogens, apoptotic cells and debris is a key feature of macrophage function in host defense and tissue homeostasis. Quantification of macrophage phagocytosis in vitro has traditionally been technically challenging. Here we report the optimization and validation of the IncuCyte ZOOM® real time imaging platform for macrophage phagocytosis based on pHrodo® pathogen bioparticles, which only fluoresce when localized in the acidic environment of the phagolysosome. Image analysis and fluorescence quantification were performed with the automated IncuCyte™ Basic Software...
September 15, 2016: Biochemical Pharmacology
https://www.readbyqxmd.com/read/27438468/chronic-calcium-channel-inhibitor-verapamil-antagonizes-tnf-%C3%AE-mediated-inflammatory-reaction-and-protects-against-inflammatory-arthritis-in-mice
#19
Wenhan Wang, Zhong Li, Qingjuan Meng, Pei Zhang, Pengcheng Yan, Zhenbiao Zhang, Hao Zhang, Jingrui Pan, Yujia Zhai, Yaoge Liu, Xiaokai Wang, Weiwei Li, Yunpeng Zhao
It is well established that the tumor necrosis factor-α (TNF-α) plays a dominant role in rheumatoid arthritis (RA). Calcium channel is recently reported to be closely associated with various inflammatory diseases. However, whether chronic calcium channel blocker verapamil plays a role in RA still remains unknown. To investigate the role of verapamil in antagonizing TNF-α-mediated inflammation reaction and the underlying mechanisms, bone marrow-derived macrophages (BMDM) cells were cultured with stimulation of TNF-α, in the presence or absence of verapamil...
October 2016: Inflammation
https://www.readbyqxmd.com/read/27406916/liver-x-receptor-and-stat1-cooperate-downstream-of-gas6-mer-to-induce-anti-inflammatory-arginase-2-expression-in-macrophages
#20
Si-Yoon Kim, Eun-Jin Lim, Young-So Yoon, Young-Ho Ahn, Eun-Mi Park, Hee-Sun Kim, Jihee Lee Kang
Mer signaling increases the transcriptional activity of liver X receptor (LXR) to promote the resolution of acute sterile inflammation. Here, we aimed to understand the pathway downstream of Mer signaling after growth arrest-specific protein 6 (Gas6) treatment that leads to LXR expression and transcriptional activity in mouse bone-marrow derived macrophages (BMDM). Gas6-induced increases in LXRα and LXRβ and expression of their target genes were inhibited in BMDM from STAT1(-/-) mice or by the STAT1-specific inhibitor fludarabine...
2016: Scientific Reports
keyword
keyword
49150
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"