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https://www.readbyqxmd.com/read/28346745/emp10-encodes-a-mitochondrial-ppr-protein-that-affects-the-cis-splicing-of-nad2-intron-1-and-seed-development-in-maize
#1
Manjun Cai, L Shuzhen, Feng Sun, Qin Sun, Hailiang Zhao, Xuemei Ren, Yanxin Zhao, Baocai Tan, Zuxin Zhang, Fazhan Qiu
In higher plants, many mitochondrial genes contain group II-type introns that are removed from RNAs by splicing to produce mature transcripts that are then translated into functional proteins. However, the factors involved in the splicing of mitochondrial introns and their biological functions are not well understood in maize. Here, we isolated an empty pericarp 10 (emp10) mutant and identified the underlying gene by map-based cloning. Emp10 encodes a P-type mitochondria-targeted pentatricopeptide repeat (PPR) protein with 10 PPR motifs...
March 27, 2017: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/28343581/proteomic-analysis-of-physiological-function-response-to-hot-summer-in-liver-from-lactating-dairy-cows
#2
Qiangjun Wang, Xiaowei Zhao, Zijun Zhang, Huiling Zhao, Dongwei Huang, Guanglong Cheng, Yongxin Yang
Lactation performance of dairy cattle is susceptible to heat stress. The liver is one of the most crucial organs affected by high temperature in dairy cows. However, the physiological adaption by the liver to hot summer conditions has not been well elucidated in lactating dairy cows. In the present study, proteomic analysis of the liver in dairy cows in spring and hot summer was performed using a label-free method. In total, 127 differentially expressed proteins were identified; most of the upregulated proteins were involved in protein metabolic processes and responses to stimuli, whereas most of the downregulated proteins were related to oxidation-reduction...
April 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/28342583/affinity-chromatographic-methodologies-based-on-immobilized-voltage-dependent-anion-channel-isoform-1-and-application-in-protein-ligand-interaction-analysis-and-bioactive-compounds-screening-from-traditional-medicine
#3
Qian Li, Pan Qiao, Xiu Chen, Jing Wang, Liujiao Bian, Xiaohui Zheng
Voltage dependent anion channel isoform 1 (VDAC-1) serves as an attractive target of anti-cancer drugs by mediating the entry and exit of metabolites between cytoplasm and mitochondria. This work reports on the preparation of a VDAC-1-based bioaffinity chromatographic stationary phase by linking the protein on lecithin modified microspheres. An assay of chromatographic methods including frontal analysis, zonal elution, injection dependent analysis and nonlinear chromatography were utilized to investigate the bindings of ATP, NADH and NADPH to VDAC-1...
March 18, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28335548/study-of-different-variants-of-mo-enzyme-crarc-and-the-interaction-with-its-partners-crcytb5-r-and-crcytb5-1
#4
Alejandro Chamizo-Ampudia, Aurora Galvan, Emilio Fernandez, Angel Llamas
The mARC (mitochondrial Amidoxime Reducing Component) proteins are recently discovered molybdenum (Mo) Cofactor containing enzymes. They are involved in the reduction of several N-hydroxylated compounds (NHC) and nitrite. Some NHC are prodrugs containing an amidoxime structure or mutagens such as 6-hydroxylaminopurine (HAP). We have studied this protein in the green alga Chlamydomonas reinhardtii (crARC). Interestingly, all the ARC proteins need the reducing power supplied by other proteins. It is known that crARC requires a cytochrome b₅ (crCytb5-1) and a cytochrome b₅ reductase (crCytb5-R) that form an electron transport chain from NADH to the substrates...
March 21, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28334681/fluorescent-proteins-such-as-egfp-lead-to-catalytic-oxidative-stress-in-cells
#5
Douglas Ganini, Fabian Leinisch, Ashutosh Kumar, JinJie Jiang, Erik J Tokar, Christine C Malone, Robert M Petrovich, Ronald P Mason
Fluorescent proteins are an important tool that has become omnipresent in life sciences research. They are frequently used for localization of proteins and monitoring of cells [1,2]. Green fluorescent protein (GFP) was the first and has been the most used fluorescent protein. Enhanced GFP (eGFP) was optimized from wild-type GFP for increased fluorescence yield and improved expression in mammalian systems [3]. Many GFP-like fluorescent proteins have been discovered, optimized or created, such as the red fluorescent protein TagRFP [4]...
March 10, 2017: Redox Biology
https://www.readbyqxmd.com/read/28332713/the-mitochondrial-pentatricopeptide-repeat-protein-ppr19-is-involved-in-the-stabilization-of-nadh-dehydrogenase-1-transcripts-and-is-crucial-for-mitochondrial-function-and-arabidopsis-thaliana-development
#6
Kwanuk Lee, Ji Hoon Han, Youn-Il Park, Catherine Colas des Francs-Small, Ian Small, Hunseung Kang
Despite the importance of pentatricopeptide repeat (PPR) proteins in organellar RNA metabolism and plant development, the functions of many PPR proteins remain unknown. Here, we determined the role of a mitochondrial PPR protein (At1g52620) comprising 19 PPR motifs, thus named PPR19, in Arabidopsis thaliana. The ppr19 mutant displayed abnormal seed development, reduced seed yield, delayed seed germination, and retarded growth, indicating that PPR19 is indispensable for normal growth and development of Arabidopsis thaliana...
March 23, 2017: New Phytologist
https://www.readbyqxmd.com/read/28327357/the-ternary-complex-structure-of-d-mandelate-dehydrogenase-with-nadh-and-anilino-oxo-acetate
#7
Nayuta Furukawa, Akimasa Miyanaga, Masahiro Nakajima, Hayao Taguchi
Enterococcus faecium NAD-dependent d-mandelate dehydrogenase (D-ManDH) belongs to a ketopantoate reductase (KPR)-related D-2-hydroxyacid dehydrogenase family, and exhibits broad substrate specificity toward bulky hydrophobic 2-ketoacids, preferring C3-branched substrates. The ternary complex structure of D-ManDH with NADH and anilino(oxo)acetate (AOA) revealed that the substrate binding induces a shear motion of the N-terminal domain along the C-terminal domain, following the hinge motion induced by the NADH binding, and allows the bound NADH molecule to form favorable interactions with a 2-ketoacid substrate...
March 18, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28319892/mitochondrial-hyperpolarization-in-ipsc-derived-neurons-from-patients-of-ftdp-17-with-10-16-mapt-mutation-leads-to-oxidative-stress-and-neurodegeneration
#8
Noemí Esteras, Jonathan D Rohrer, John Hardy, Selina Wray, Andrey Y Abramov
Tau protein inclusions are a frequent hallmark of a variety of neurodegenerative disorders. The 10+16 intronic mutation in MAPT gene, encoding tau, causes frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17), by altering the splicing of the gene and inducing an increase in the production of 4R tau isoforms, which are more prone to aggregation. However, the molecular mechanisms linking increased 4R tau to neurodegeneration are not well understood. Here, we have used iPSC-derived neurons from patients of FTDP-17 carrying the 10+16 mutation to study the molecular mechanisms underlying neurodegeneration...
March 10, 2017: Redox Biology
https://www.readbyqxmd.com/read/28318242/whole-blood-metabolomics-by-1h-nmr-spectroscopy-provides-a-new-opportunity-to-evaluate-coenzymes-and-antioxidants
#9
G A Nagana Gowda, Daniel Raftery
Conventional human blood metabolomics employs serum or plasma and provides a wealth of metabolic information therein. However, this approach lacks the ability to measure and evaluate important metabolites such as coenzymes that are present at high concentrations in red blood cells. As an important alternative to serum/plasma metabolomics, we show here that a simple 1H NMR experiment can simultaneously measure coenzymes and antioxidants in extracts of whole human blood, in addition to the nearly 70 metabolites that were shown to be quantitated in serum/plasma, recently [Anal...
March 20, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28315593/ingan-gan-nanowires-as-a-new-platform-for-photoelectrochemical-sensors-detection-of-nadh
#10
M Riedel, S Hölzel, P Hille, J Schörmann, M Eickhoff, F Lisdat
InGaN/GaN nanowire heterostructures are presented as nanophotonic probes for the light-triggered photoelectrochemical detection of NADH. We demonstrate that photogenerated electron-hole pairs give rise to a stable anodic photocurrent whose potential- and pH-dependences exhibit broad applicability. In addition, the simultaneous measurement of the photoluminescence provides an additional tool for the analysis and evaluation of light-triggered reaction processes at the nanostructured interface. InGaN/GaN nanowire ensembles can be excited over a wide wavelength range, which avoids interferences of the photoelectrochemical response by absorption properties of the compounds to be analyzed by adjusting the excitation wavelength...
March 11, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28315482/modes-of-antibacterial-action-of-curcumin-under-dark-and-light-conditions-a-toxicoproteomics-approach
#11
Ilya Shlar, Samir Droby, Victor Rodov
Curcumin is a potent natural food-grade antimicrobial compound. Exposure to light further enhances its antimicrobial capacity. Proteomic methods were used in this study for investigating the mechanistic aspects of the antibacterial curcumin effects in the dark and upon illumination. Escherichia coli cells exposed to water-dispersible curcumin-methyl-β-cyclodextrin inclusion complex under dark and light conditions were compared with the non-treated cells kept under the same illumination regimes. Curcumin treatment in the dark evoked adaptive responses aimed at mitigation of oxidative stress, DNA protection, proteostasis, modulation of redox state via changing NADH level, and gasotransmitter (H2S and NH3) biosynthesis...
March 14, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28315453/defects-in-mitochondrial-energetic-function-compels-fanconi-anemia-cells-to-glycolytic-metabolism
#12
Enrico Cappelli, Paola Cuccarolo, Giorgia Stroppiana, Maurizio Miano, Roberta Bottega, Vanessa Cossu, Paolo Degan, Silvia Ravera
Energetic metabolism plays an essential role in the differentiation of haematopoietic stem cells (HSC). In Fanconi Anaemia (FA), DNA damage is accumulated during HSC differentiation, an event that is likely associated with bone marrow failure (BMF). One of the sources of the DNA damage is altered mitochondrial metabolism and an associated increment of oxidative stress. Recently, altered mitochondrial morphology and a deficit in the energetic activity in FA cells have been reported. Considering that mitochondria are the principal site of aerobic ATP production, we investigated FA metabolism in order to understand what pathways are able to compensate for this energy deficiency...
March 14, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28304162/rational-design-synthesis-and-biological-evaluation-of-heterocyclic-quinolones-targeting-the-respiratory-chain-of-mycobacterium-tuberculosis
#13
Weiqian David Hong, Peter D Gibbons, Suet C Leung, Richard Amewu, Paul A Stocks, Andrew Valentine Stachulski, Pedro C Horta, Maria Lurdes Santos Cristiano, Alison E Shone, Darren Moss, Alison Ardrey, Raman Sharma, Ashley J Warman, Paul T P Bedingfield, Nicholas E Fisher, Ghaith Aljayyoussi, Sally Mead, Maxine Caws, Neil G Berry, Stephen A Ward, Giancarlo A Biagini, Paul M O'Neill, Gemma Louise Nixon
A High-throughput screen (HTS) was undertaken against the respiratory chain dehydrogenase component, NADH:menaquinone oxidoreductase (Ndh) of Mycobacterium tuberculosis (Mtb). 11,000 compounds were selected for the HTS based on the known phenothiazine Ndh inhibitors, trifluoperazine and thioridazine. Combined HTS (11,000 compounds) and in-house screening of a limited number of quinolones (50 compounds) identified ~100 hits and four distinct chemotypes, the most promising of which contained the quinolone core...
March 17, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28303934/redox-sensing-regulator-rex-regulates-aerobic-metabolism-morphological-differentiation-and-avermectin-production-in-streptomyces-avermitilis
#14
Xingchao Liu, Yaqing Cheng, Mengya Lyu, Ying Wen, Yuan Song, Zhi Chen, Jilun Li
The regulatory role of redox-sensing regulator Rex was investigated in Streptomyces avermitilis. Eleven genes/operons were demonstrated to be directly regulated by Rex; these genes/operons are involved in aerobic metabolism, morphological differentiation, and secondary metabolism. Rex represses transcription of target genes/operons by binding to Rex operator (ROP) sequences in the promoter regions. NADH reduces DNA-binding activity of Rex to target promoters, while NAD(+) competitively binds to Rex and modulates its DNA-binding activity...
March 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28303650/enzymatic-electrosynthesis-of-formic-acid-through-co2-reduction-in-bioelectrochemical-system-bes-effect-of-immobilization-and-carbonic-anhydrase-addition
#15
Sandipam Srikanth, Yolanda Alvarez Gallego, Karolien Vanbroekhoven, Deepak Pant
Enzymatic electrosynthesis of formic acid from carbon dioxide (CO2) reduction using formate dehydrogenase (FDH) as catalyst at cathode both in its free and immobilized forms was studied in detail in bioelectrochemical system (BES). The essential role of solubilizing CO2 for its conversion was also studied by adding carbonic anhydrase (CA) to the FDH enzyme both in free and immobilized forms. FDH alone in the free form showed large variation in reduction current (-6.2±3.9 A/m2), while the immobilized form showed less variation (-3...
March 17, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/28303408/methylene-blue-decreases-mitochondrial-lysine-acetylation-in-the-diabetic-heart
#16
Jessica M Berthiaume, Chia-Heng Hsiung, Alison B Austin, Sean P McBrayer, Mikayla M Depuydt, Margaret P Chandler, Masaru Miyagi, Mariana G Rosca
Diabetic cardiomyopathy is preceded by mitochondrial alterations, and progresses to heart failure. We studied whether treatment with methylene blue (MB), a compound that was reported to serve as an alternate electron carrier within the mitochondrial electron transport chain (ETC), improves mitochondrial metabolism and cardiac function in type 1 diabetes. MB was administered at 10 mg/kg/day to control and diabetic rats. Both echocardiography and hemodynamic studies were performed to assess cardiac function...
March 16, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28303352/redirecting-carbon-flux-through-pgi-deficient-and-heterologous-transhydrogenase-toward-efficient-succinate-production-in-corynebacterium-glutamicum
#17
Chen Wang, Zhihui Zhou, Heng Cai, Zhongjun Chen, Hongtao Xu
Corynebacterium glutamicum is particularly known for its potentiality in succinate production. We engineered C. glutamicum for the production of succinate. To enhance C3-C4 carboxylation efficiency, chromosomal integration of the pyruvate carboxylase gene pyc resulted in strain NC-4. To increase intracellular NADH pools, the pntAB gene from Escherichia coli, encoding for transhydrogenase, was chromosomally integrated into NC-4, leading to strain NC-5. Furthermore, we deleted pgi gene in strain NC-5 to redirect carbon flux to the pentose phosphate pathway (PPP)...
March 16, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28298443/a-four-gene-operon-in-bacillus-cereus-produces-two-rare-spore-decorating-sugars
#18
Zi Li, Thiya Mukherjee, Kyle Bowler, Sholeh Namdari, Zachary Snow, Sarah Prestridge, Alexandra Carlton, Maor Bar-Peled
Bacterial glycan structures on cell surfaces are critical for cell-cell recognition and adhesion and in host-pathogen interactions. Accordingly, unraveling the sugar composition of bacterial cell surfaces can shed light on bacterial growth and pathogenesis. Here, we found that two rare sugars with a 3-C-methyl-6-deoxy-hexose structure were linked to spore glycan in Bacillus cereus ATCC 14579 and ATCC 10876. Moreover, we identified a four-gene operon in B. cereus ATCC 14579 that encodes proteins with the following sequential enzyme activities as determined by mass spectrometry and one- and two-dimensional NMR methods: CTP:glucose-1-phosphate cytidylyltransferase, a CDP-Glc 4,6-dehydratase, an NADH-dependent SAM:C-methyltransferase, and an NADPH-dependent CDP-3-C-methyl-6-deoxy-hexose 4-reductase...
March 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28298441/identification-of-the-binding-sites-for-ubiquinone-and-inhibitors-in-the-na-pumping-nadh-ubiquinone-oxidoreductase-of-vibrio-cholerae-by-photoaffinity-labeling
#19
Takeshi Ito, Masatoshi Murai, Satoshi Ninokura, Yuki Kitazumi, Katherine G Mezic, Brady F Cress, Mattheos A G Koffas, Joel E Morgan, Blanca Barquera, Hideto Miyoshi
The Na(+)-pumping NADH-quinone oxidoreductase (Na(+)-NQR) is the first enzyme of the respiratory chain and the main ion transporter in many marine and pathogenic bacteria, including Vibrio cholerae The V. cholerae Na(+)-NQR has been extensively studied, but its binding sites for ubiquinone and inhibitors remain controversial. Here, using a photoreactive ubiquinone PUQ-3 as well as two aurachin-type inhibitors [(125)I]PAD-1 and [(125)I]PAD-2 and photoaffinity labeling experiments on the isolated enzyme, we demonstrate that the ubiquinone ring binds to the NqrA subunit in the regions Leu32-Met39 and Phe131-Lys138, encompassing the rear wall of a predicted ubiquinone-binding cavity...
March 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28296386/direct-drawing-method-of-graphite-onto-paper-for-high-performance-flexible-electrochemical-sensors
#20
Murilo Santhiago, Mathias Strauss, Mariane P Pereira, Andréia S Chagas, Carlos C B Bufon
A simple and fast fabrication method to create high-performance pencil-drawn electrochemical sensors is reported for the first time. The sluggish electron transfer observed on bare pencil-drawn surfaces was enhanced using two electrochemical steps: first oxidizing the surface and then reducing it in a subsequent step. The heterogeneous rate constant was found to be 5.1 × 10(-3) cm s(-1), which is the highest value reported so far for pencil-drawn surfaces. We mapped the origin of such performance by atomic force microscopy, X-ray photoelectron spectroscopy, and Raman spectroscopy...
March 22, 2017: ACS Applied Materials & Interfaces
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