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https://www.readbyqxmd.com/read/28220137/proteomic-analysis-reveals-the-positive-effect-of-exogenous-spermidine-in-tomato-seedlings-response-to-high-temperature-stress
#1
Qinqin Sang, Xi Shan, Yahong An, Sheng Shu, Jin Sun, Shirong Guo
Polyamines are phytohormones that regulate plant growth and development as well as the response to environmental stresses. To evaluate their functions in high-temperature stress responses, the effects of exogenous spermidine (Spd) were determined in tomato leaves using two-dimensional electrophoresis and MALDI-TOF/TOF MS. A total of 67 differentially expressed proteins were identified in response to high-temperature stress and/or exogenous Spd, which were grouped into different categories according to biological processes...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28219823/proteomic-analysis-of-a-carbapenem-resistant-klebsiella-pneumoniae-strain-in-response-to-meropenem-stress
#2
Arbab Khan, Divakar Sharma, Mohammad Faheem, Deepa Bisht, Asad U Khan
OBJECTIVES: Antibiotic resistance has become a major problem in treating bacterial infections. The aim of this study was to elucidate the effects of meropenem on a blaKPC-2-harbouring multidrug-resistant clinical strain of Klebsiella pneumoniae through a proteomics approach in order to attain a deeper understanding of bacterial resistance strategies. METHODS: Analysis was performed by two-dimensional gel electrophoresis of whole-cell extracts of bacteria exposed to a sublethal concentration of meropenem compared with the untreated control...
February 17, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28219754/identification-of-responsive-proteins-in-panonychus-citri-exposed-to-abamectin-by-a-proteomic-approach
#3
Xiao-Min Shen, Rui Zhong, Wen-Kai Xia, Dong Wei, Tian-Bo Ding, Chong-Yu Liao, Jin-Zhi Niu, Wei Dou, Jin-Jun Wang
: Abamectin is a microbial-derived pesticide widely used for control of agricultural pests. However, sustained use of abamectin has led to the development of resistance in some target species. Previous studies on arthropod resistance to abamectin have mainly used traditional biochemical and molecular approaches. To understand the responses of citrus red mite, Panonychus citri, exposed to abamectin, comparative proteomic analysis was conducted using two-dimensional electrophoresis (2-DE)...
February 17, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28207789/carbapenem-resistance-and-pathogenicity-of-bovine-acinetobacter-indicus-like-isolates
#4
Peter Klotz, Stephan Göttig, Ursula Leidner, Torsten Semmler, Sandra Scheufen, Christa Ewers
The objective of this study was to characterize blaOXA-23 harbouring Acinetobacter indicus-like strains from cattle including genomic and phylogenetic analyses, antimicrobial susceptibility testing and evaluation of pathogenicity in vitro and in vivo. Nasal and rectal swabs (n = 45) from cattle in Germany were screened for carbapenem-non-susceptible Acinetobacter spp. Thereby, two carbapenem resistant Acinetobacter spp. from the nasal cavities of two calves could be isolated. MALDI-TOF mass spectrometry and 16S rDNA sequencing identified these isolates as A...
2017: PloS One
https://www.readbyqxmd.com/read/28192992/protein-adsorbed-magnetic-nanoparticle-mediated-assay-for-rapid-detection-of-bacterial-antibiotic-resistance
#5
Taku A Cowger, Yaping Yang, David E Rink, Trever Todd, Hongmin Chen, Ye Shen, Yajun Yan, Jin Xie
Antibiotic susceptibility tests have been used for years as a crucial diagnostic tool against antibiotic-resistant bacteria. However, due to a lack of biomarkers specific to resistant types, these approaches are often time-consuming, inaccurate, and inflexible in drug selections. Here, we present a novel susceptibility test method named protein-adsorbed nanoparticle-mediated matrix-assisted laser desorption-ionization mass spectrometry, or PANMS. Briefly, we adsorb five different proteins (β-casein, α-lactalbumin, human serum albumin, fibrinogen, and avidin) onto the surface of Fe3O4...
February 17, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28173787/first-time-identification-of-pandoraea-sputorum-from-a-patient-with-cystic-fibrosis-in-argentina-a-case-report
#6
Pablo F Martina, Mónica Martínez, Guillermo Frada, Florencia Alvarez, Lorena Leguizamón, Claudia Prieto, Carolina Barrias, Marisa Bettiol, Antonio Lagares, Alejandra Bosch, Julián Ferreras, Martha Von Specht
BACKGROUND: Pandoraea species are considered emerging pathogens in the context of cystic fibrosis (CF) and are difficult to identify by conventional biochemical methods. These multidrug resistant bacteria remain poorly understood particularly in terms of natural resistance, mechanisms of acquired resistance and impact on the prognosis of the disease and the lung function. Among them, Pandoraea sputorum has been previously described in few cases of CF patients from Spain, Australia, France and United States, underlining the need of more clinical data for a better knowledge of its pathogenicity...
February 7, 2017: BMC Pulmonary Medicine
https://www.readbyqxmd.com/read/28170113/helicoverpa-zea-gut-associated-bacteria-indirectly-induce-defenses-in-tomato-by-triggering-a-salivary-elicitor-s
#7
Jie Wang, Michelle Peiffer, Kelli Hoover, Cristina Rosa, Rensen Zeng, Gary W Felton
Insect gut-associated microbes modulating plant defenses have been observed in beetles and piercing-sucking insects, but the role of caterpillar-associated bacteria in regulating plant induced defenses has not been adequately examined. We identified bacteria from the regurgitant of field-collected Helicoverpa zea larvae using 16S ribosomal RNA (rRNA) gene sequencing and matrix-assisted laser desorption/ionization time of flight (MALDI-TOF) mass spectrometry. A combination of biochemical, molecular, and confocal electron microscopy methods were used to determine the role of caterpillar-associated bacteria in mediating defenses in Solanum lycopersicum (tomato)...
February 7, 2017: New Phytologist
https://www.readbyqxmd.com/read/28161294/pantoea-calida-bacteremia-in-an-adult-with-end-stage-stomach-cancer-under-inpatient-care
#8
Kageto Yamada, Machiko Kashiwa, Katsumi Arai, Kazuya Satoyoshi, Hiroyuki Nishiyama
Pantoea calida is a gram-negative bacillus that was first identified in 2010. Here, we describe the first known case of P. calida bacteremia in a 77-year-old woman with end-stage stomach cancer under inpatient care. The patient was admitted to our hospital for pain after receiving anti-cancer therapy at outpatient facility. Thirteen days after admission, her temperature rose to 39.6 °C. A blood culture was ordered for suspected bacterial infection, and the patient was treated empirically with ampicillin/sulbactam...
February 2, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28146197/-chronic-osteomyelitis-due-to-corynebacterium-striatum-in-a-female-adolescent
#9
Claudia Beltrán-Arroyave, Alejandro Díaz-Díaz, Natalia Loaiza-Díaz
A case of a girl with chronic posttraumatic osteomyelitis of the right tibia with microbiological isolation of Bacillus spp. by conventional methods and confirmation by mass spectrometry MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight mass) of Corynebacterium striatum is presented. Diagnostic methods, clinical manifestations, and resistance pattern of these infections are described.
December 2016: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
https://www.readbyqxmd.com/read/28135327/characterization-of-multidrug-resistant-e-faecalis-strains-from-pigs-of-local-origin-by-adsrrs-fingerprinting-and-maldi-tof-ms-evaluation-of-the-compatibility-of-methods-employed-for-multidrug-resistance-analysis
#10
Aneta Nowakiewicz, Grażyna Ziółkowska, Przemysław Zięba, Sebastian Gnat, Aleksandra Trościańczyk, Łukasz Adaszek
The aim of this study was to characterize multidrug resistant E. faecalis strains from pigs of local origin and to analyse the relationship between resistance and genotypic and proteomic profiles by amplification of DNA fragments surrounding rare restriction sites (ADSRRS-fingerprinting) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI -TOF MS). From the total pool of Enterococcus spp. isolated from 90 pigs, we selected 36 multidrug resistant E. faecalis strains, which represented three different phenotypic resistance profiles...
2017: PloS One
https://www.readbyqxmd.com/read/28134321/rapid-sputum-multiplex-detection-of-the-m-tuberculosis-complex-mtbc-and-resistance-mutations-for-eight-antibiotics-by-nucleotide-maldi-tof-ms
#11
Kang-Yi Su, Bo-Shiun Yan, Hao-Chieh Chiu, Chong-Jen Yu, So-Yi Chang, Ruwen Jou, Jia-Long Liu, Po-Ren Hsueh, Sung-Liang Yu
The increasing incidence of multidrug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis (MTB) adds further urgency for rapid and multiplex molecular testing to identify the MTB complex and drug susceptibility directly from sputum for disease control. A nucleotide matrix-assisted-laser-desorption-ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based assay was developed to identify MTB (MTBID panel) and 45 chromosomal mutations for resistance to eight antibiotics (MTBDR panel)...
January 30, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28122803/a-decade-of-development-of-chromogenic-culture-media-for-clinical-microbiology-in-an-era-of-molecular-diagnostics
#12
REVIEW
John D Perry
In the last 25 years, chromogenic culture media have found widespread application in diagnostic clinical microbiology. In the last decade, the range of media available to clinical laboratories has expanded greatly, allowing specific detection of additional pathogens, including Pseudomonas aeruginosa, group B streptococci, Clostridium difficile, Campylobacter spp., and Yersinia enterocolitica. New media have also been developed to screen for pathogens with acquired antimicrobial resistance, including vancomycin-resistant enterococci, carbapenem-resistant Acinetobacter spp...
April 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/28119478/rapid-direct-detection-of-carbapenemase-producing-enterobacteriaceae-in-clinical-urine-samples-by-maldi-tof-ms-analysis
#13
Marina Oviaño, Cecilia de la Luna Ramírez, Luis Pedro Barbeyto, Germán Bou
OBJECTIVES: Development of an automated MALDI-TOF MS-based method for the rapid, direct detection of carbapenemase-producing Enterobacteriaceae in clinical urine samples within 90 min of sample reception. METHODS: A total of 3041 urine samples were processed by flow cytometry, and a cut-off value of ≥1.5 × 10(5) bacteria/mL was used to select samples for the study. Following these criteria, 608 samples were selected for direct bacterial identification...
January 23, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28116632/itraq-based-quantitative-proteomic-analysis-on-differentially-expressed-proteins-of-rat-mandibular-condylar-cartilage-induced-by-reducing-dietary-loading
#14
Liting Jiang, Yinyin Xie, Li Wei, Qi Zhou, Ning Li, Xinquan Jiang, Yiming Gao
As muscle activity during growth is considerably important for mandible quality and morphology, reducing dietary loading directly influences the development and metabolic activity of mandibular condylar cartilage (MCC). However, an overall investigation of changes in the protein composition of MCC has not been fully described in literature. To study the protein expression and putative signaling in vivo, we evaluated the structural changes of MCC and differentially expressed proteins induced by reducing functional loading in rat MCC at developmental stages...
January 23, 2017: Frontiers of Medicine
https://www.readbyqxmd.com/read/28109846/antimicrobial-susceptibility-of-clinical-isolates-of-actinomyces-and-related-genera-reveals-an-unusual-clindamycin-resistance-among-actinomyces-urogenitalis-strains
#15
Claudia Barberis, Mabel Budia, Susana Palombarani, Carlos Hernán Rodriguez, María Soledad Ramírez, Barbara Arias, Laura Bonofiglio, Angela Famiglietti, Marta Mollerach, Marisa Almuzara, Carlos Vay
OBJECTIVES: Patterns of antimicrobial susceptibility in Actinomyces and related genera are very limited in the literature. Data of predominant susceptibility profiles could contribute to the establishment of an accurate empirical treatment. METHODS: A total of 113 isolates from clinical samples were included in this study. Each isolate was identified using phenotypic methods and MALDI-TOF/MS. When discrepancies were observed, 16S rRNA gene sequencing was performed...
January 18, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28109552/-rapid-methods-for-detection-of-bacterial-resistance-to-antibiotics
#16
Gabriel Alberto March-Rosselló
The most widely used antibiotic susceptibility testing methods in Clinical Microbiology are based on the phenotypic detection of antibiotic resistance by measuring bacterial growth in the presence of the antibiotic being tested. These conventional methods take typically 24hours to obtain results. Here we review the main techniques for rapid determination of antibiotic susceptibility. Data obtained with different methods such as molecular techniques, microarrays, commercial methods used in work routine, immunochromatographic methods, colorimetric methods, image methods, nephelometry, MALDI-TOF mass spectrometry, flow cytometry, chemiluminescence and bioluminescence, microfluids and methods based on cell disruption are analysed in detail...
January 18, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28108122/-fast-methods-of-fungal-and-bacterial-identification-maldi-tof-mass-spectrometry-chromogenic-media
#17
María Siller-Ruiz, Sara Hernández-Egido, Fernando Sánchez-Juanes, José Manuel González-Buitrago, Juan Luis Muñoz-Bellido
MALDI-TOF mass spectrometry is now a routine resource in Clinical Microbiology, because of its speed and reliability in the identification of microorganisms. Its performance in the identification of bacteria and yeasts is perfectly contrasted. The identification of mycobacteria and moulds is more complex, due to the heterogeneity of spectra within each species. The methodology is somewhat more complex, and expanding the size of species libraries, and the number of spectra of each species, will be crucial to achieve greater efficiency...
January 17, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28098550/determination-of-elizabethkingia-diversity-by-maldi-tof-mass-spectrometry-and-whole-genome-sequencing
#18
Helle Brander Eriksen, Heidi Gumpert, Cecilie Haase Faurholt, Henrik Westh
In a hospital-acquired infection with multidrug-resistant Elizabethkingia, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rRNA gene analysis identified the pathogen as Elizabethkingia miricola. Whole-genome sequencing, genus-level core genome analysis, and in silico DNA-DNA hybridization of 35 Elizabethkingia strains indicated that the species taxonomy should be further explored.
February 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28092721/use-of-maldi-tof-mass-spectrometry-in-the-battle-against-bacterial-infectious-diseases-recent-achievements-and-future-perspectives
#19
Vassilios Sandalakis, Ioannis Goniotakis, Iosif Vranakis, Dimosthenis Chochlakis, Anna Psaroulaki
Advancements in microbial identification occur increasingly faster as more laboratories explore, refine and extend the use of mass spectrometry in the field of microbiology. Areas covered: This review covers the latest knowledge found in the literature for quick identification of various classes of bacterial pathogens known to cause human infection by the use of MALDI-TOF MS technology. Except for identification of bacterial strains, more researchers try to 'battle time' in favor of the patient. These novel approaches to identify bacteria directly from clinical samples and even determine antibiotic resistance are extensively revised and discussed...
January 22, 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/28091797/mass-spectrometric-identification-of-phenol-soluble-modulins-in-the-atcc%C3%A2-43300-standard-strain-of-methicillin-resistant-staphylococcus-aureus-harboring-two-distinct-phenotypes
#20
K S Jang, M Park, J Y Lee, J S Kim
Staphylococcus aureus subsp. aureus Rosenbach (ATCC® 43300™) is a representative methicillin-resistant S. aureus (MRSA) strain that is used as a quality control strain for testing the methicillin susceptibility of clinical isolates. It has been consistently observed that colonies with two different colors (golden yellow and white) grow from the ATCC stock on blood agar plates. In this study, staphylococcal peptide and protein profiling was performed using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry...
January 14, 2017: European Journal of Clinical Microbiology & Infectious Diseases
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