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https://www.readbyqxmd.com/read/28921905/antibiotic-resistant-escherichia-coli-in-southeastern-australian-pig-herds-and-implications-for-surveillance
#1
L K van Breda, O P Dhungyel, M P Ward
To investigate public health implications of antibiotics to control post-weaning scours, we surveyed 22 commercial pig herds in southeastern Australia. Fifty faecal samples per herd were collected from pre- and post-weaned piglets. Presumptive Escherichia coli isolates were confirmed by MALDI-TOF MS. Isolates (n = 325) were screened for susceptibility to 19 veterinary antibiotics using MIC broth microdilution. All 325 E. coli isolates underwent further testing against 27 antibiotics used in human medicine and were screened for ETEC adhesin and enterotoxin genes (F4 (K88), F5 (K99), F6 (987P), F18, F41, STa, STb, Stx2e and LT) by multiplex PCR...
September 17, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28904565/class-1-integrons-and-plasmid-mediated-multiple-resistance-genes-of-the-campylobacter-species-from-pediatric-patient-of-a-university-hospital-in-taiwan
#2
Yi-Chih Chang, Ni Tien, Jai-Sing Yang, Chi-Cheng Lu, Fuu-Jen Tsai, Tsurng-Juhn Huang, I-Kuan Wang
BACKGROUND: The Campylobacter species usually causes infection between humans and livestock interaction via livestock breeding. The studies of the Campylobacter species thus far in all clinical isolates were to show the many kinds of antibiotic phenomenon that were produced. Their integrons cause the induction of antibiotic resistance between bacterial species in the Campylobacter species. RESULTS: The bacterial strains from the diarrhea of pediatric patient which isolated by China Medical University Hospital storage bank...
2017: Gut Pathogens
https://www.readbyqxmd.com/read/28903168/-sharing-bacterial-microbiota-between-owners-and-their-pets-dogs-cats
#3
Jan Wipler, Zuzana Čermáková, Tomáš Hanzálek, Hana Horáková, Helena Žemličková
INTRODUCTION: The microbiological aspect of a relationship between pets (dogs/cats) and their owners is mainly concerned with the incidence of shared bacterial species, in particular potential pathogens. Given the great popularity of sharing homes with pets (dogs/cats) in the Czech Republic, there is an increased possibility of communication between microbiota of the two macroorganisms (pet and owner). The aim of the study was to determine the biodiversity of shared bacteria and possibility of exchange of genes of resistance to antimicrobial agents between potential pathogens based on the close relationship between pets and humans...
June 2017: Klinická Mikrobiologie a Infekc̆ní Lékar̆ství
https://www.readbyqxmd.com/read/28900246/maldi-tof-ms-based-identification-and-molecular-characterization-of-food-associated-methicillin-resistant-staphylococcus-aureus
#4
H M Manukumar, S Umesha
Food-borne methicillin resistant Staphylococcus aureus (MRSA) is involved in two-fold higher mortality rate compared to methicillin susceptible S. aureus (MSSA). Eventhough Mysuru recognized as cleanest city in the world, prevalence of food contamination is not detailed. The aim is to screen food samples from Mysuru area and to characterize MRSA strain, employing MALDI-Biotyper, multiplex PCR to distinguish between MRSA and MSSA by PCR-coupled single strand conformation polymorphism (PCR-SSCP). Of all the food-borne pathogens, S...
September 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28895046/characterization-of-cadmium-resistant-klebsiella-pneumoniae-mcc-3091-promoted-rice-seedling-growth-by-alleviating-phytotoxicity-of-cadmium
#5
Krishnendu Pramanik, Soumik Mitra, Anumita Sarkar, Tithi Soren, Tushar Kanti Maiti
Cadmium (Cd) phytotoxicity in agricultural land is a major global concern now-a-days resulting in very poor yield. Plant growth-promoting rhizobacteria (PGPR)-mediated bioremediation is one of the convenient strategies for detoxification of Cd from the soil and for plant growth promotion under Cd stress. The selected strain K5 was identified as Klebsiella pneumoniae based on MALDI-TOF MS ribosomal protein and 16S rDNA sequence-based homology. The strain possessed several PGP traits viz. IAA production (3413 μg/mL), phosphate solubilization (80...
September 11, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28887200/purification-and-characterization-of-a-novel-anti-staphylococcal-peptide-asp-1-from-bacillus-sp-urid-12-1
#6
Ajay Ghosh Chalasani, Utpal Roy, Sushma Nema
A strong anti-methicillin resistant Staphylococcus aureus (MRSA) peptide (ASP-1) from the Bacillus subtilis URID 12.1 strain that is active against cefoxitin- and methicillin-resistant clinical isolates was purified to homogeneity by solvent extraction, silica gel-based adsorption chromatography and reversed phase High Performance Liquid Chromatography. The peptide sequence of ASP-1 as determined by MALDI-TOF/MS and ESI-FTICR MS was acetylated Phe-Thr-Ala-Val-Dhb-Phe-Ile/Leu. The peptide was further analyzed by alkaline hydrolysis, ESI-Q-TOF-MS and an ion mobility assay, which detected the presence of a lactone ring in the intact peptide and a cyclic nature, subsequently revealing the peptide sequence as acPhe-Thr-Val-Ala-Dhb-Phe-Ile/Leu...
September 5, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28859120/semi-quantitative-maldi-tof-for-antimicrobial-susceptibility-testing-in-staphylococcus-aureus
#7
Tucker Maxson, Cheryl L Taylor-Howell, Timothy D Minogue
Antibiotic resistant bacterial infections are a significant problem in the healthcare setting, in many cases requiring the rapid administration of appropriate and effective antibiotic therapy. Diagnostic assays capable of quickly and accurately determining the pathogen resistance profile are therefore crucial to initiate or modify care. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is a standard method for species identification in many clinical microbiology laboratories and is well positioned to be applied towards antimicrobial susceptibility testing...
2017: PloS One
https://www.readbyqxmd.com/read/28856505/comparative-analysis-of-constitutive-proteome-between-resistant-and-susceptible-tomato-genotypes-regarding-to-late-blight
#8
Bruno Soares Laurindo, Renata Dias Freitas Laurindo, Patrícia Pereira Fontes, Camilo Elber Vital, Fábio Teixeira Delazari, Maria Cristina Baracat-Pereira, Derly José Henriques da Silva
Late blight is one of the most destructive diseases of the tomato, resulting in substantial economic losses. There is difficulty in controlling this disease, so the molecular characterization of tomato genotypes may help in the selection of higher resistance tomato plants against Phytophthora infestans, late blight's pathogen. The objective was to analyze the differences with regard to the constitutive proteome between the access Vegetable Germplasm Bank (BGH)-2127, resistant genotype, and Santa Clara-susceptible genotype to late blight...
August 30, 2017: Functional & Integrative Genomics
https://www.readbyqxmd.com/read/28856222/comparison-of-clinical-methods-for-detecting-carbapenem-resistant-enterobacteriaceae
#9
Kirsten Kost, Jumi Yi, Beverly Rogers, Robert Jerris
We evaluated detection of carbapenem-resistant Enterobacteriaceae (CRE) by routine minimal inhibitory concentration (MIC) testing, polymerase chain reaction (PCR) using Xpert® Carba-R assay, hydrolysis of ertapenem and imipenem detected by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS), and hydrolysis by colorimetry using the EPI-CRE assay. Ninety-six Enterobacteriaceae isolates possessing carbapenemase genes and 29 carbapenem-susceptible Enterobacteriaceae were available for testing...
August 2017: Pract Lab Med
https://www.readbyqxmd.com/read/28831593/impact-of-rapid-diagnosis-of-staphylococcus-aureus-bacteremia-from-positive-blood-cultures-on-patient-management
#10
M P Romero-Gómez, E Cendejas-Bueno, J García Rodriguez, J Mingorance
We have performed a retrospective, before-after comparison of turnaround time and therapy adjustment parameters before and after the introduction of matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) plus mecA polymerase chain reaction (PCR) for the identification of methicillin-resistant Staphylococcus aureus (MRSA) in positive blood cultures. There were 227 episodes of S. aureus bacteremia during the study periods. The pre-MALDI-TOF and post-MALDI-TOF groups included 133 and 94 patients, respectively...
August 23, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28831086/potential-use-of-maldi-tof-mass-spectrometry-for-rapid-detection-of-antifungal-resistance-in-the-human-pathogen-candida-glabrata
#11
Antonietta Vella, Elena De Carolis, Enrica Mello, David S Perlin, Dominique Sanglard, Maurizio Sanguinetti, Brunella Posteraro
The echinocandins are relatively new antifungal drugs that represent, together with the older azoles, the recommended and/or preferred agents to treat candidaemia and other forms of invasive candidiasis in human patients. If "time is of the essence" to reduce the mortality for these infections, the administration of appropriate antifungal therapy could be accelerated by the timely reporting of laboratory test results. In this study, we attempted to validate a MALDI-ToF mass spectrometry-based assay for the antifungal susceptibility testing (AFST) of the potentially multidrug-resistant pathogen Candida glabrata against anidulafungin and fluconazole...
August 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28826423/pseudomonas-aeruginosa-blood-stream-infection-isolates-from-patients-with-recurrent-blood-stream-infection-is-it-the-same-genotype
#12
K L McCARTHY, T J Kidd, D L Paterson
The type identity of strains of Pseudomonas aeruginosa from primary and recurrent blood stream infection (BSI) has not been widely studied. Twenty-eight patients were identified retrospectively from 2008 to 2013 from five different laboratories; available epidemiological, clinical and microbiological data were obtained for each patient. Isolates were genotyped by iPLEX MassARRAY MALDI-TOF MS and rep-PCR. This showed that recurrent P. aeruginosa BSI was more commonly due to the same genotypically related strain as that from the primary episode...
August 22, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28811086/proteomic-profiling-of-the-antifungal-drug-response-of-aspergillus-fumigatus-to-voriconazole
#13
Nansalmaa Amarsaikhan, Daniela Albrecht-Eckardt, Christoph Sasse, Gerhard H Braus, Zumrut B Ogel, Olaf Kniemeyer
Antifungal resistance is an emerging problem and one of the reasons for treatment failure of invasive aspergillosis (IA). Voriconazole has become a standard therapeutic for the treatment of this often fatal infection. We studied the differentially expressed proteins as a response of Aspergillus fumigatus to voriconazole by employing the two-dimensional difference gel electrophoresis (DIGE) technique. Due to addition of drug, a total of 135 differentially synthesized proteins were identified by MALDI-TOF/TOF-mass spectrometry...
August 5, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28795274/novel-gemcitabine-conjugated-albumin-nanoparticles-a-potential-strategy-to-enhance-drug-efficacy-in-pancreatic-cancer-treatment
#14
Varun Kushwah, Ashish Kumar Agrawal, Chander Parkash Dora, David Mallinson, Dimitrios A Lamprou, Ramesh C Gupta, Sanyog Jain
PURPOSE: The present study reports a novel conjugate of gemcitabine (GEM) with bovine serum albumin (BSA) and thereof nanoparticles (GEM-BSA NPs) to potentiate the therapeutic efficacy by altering physicochemical properties, improving cellular uptake and stability of GEM. METHODS: The synthesized GEM-BSA conjugate was extensively characterized by NMR, FTIR, MALDI-TOF and elemental analysis. Conjugation mediated changes in structural conformation and physicochemical properties were analysed by fluorescence, Raman and CD spectroscopy, DSC and contact angle analysis...
August 9, 2017: Pharmaceutical Research
https://www.readbyqxmd.com/read/28780309/clinical-and-microbiological-characteristics-of-pantoea-agglomerans-infection-in-children
#15
Ayşe Büyükcam, Özlem Tuncer, Deniz Gür, Banu Sancak, Mehmet Ceyhan, Ali Bülent Cengiz, Ateş Kara
Pantoea agglomerans is an environmental Gram-negative bacterium that rarely is responsible for the infections in humans but it is often a causative factor of a number of occupational diseases. This study evaluated the clinical and microbiological characteristics and pathogenicity of P. agglomerans in children. We retrospectively reviewed microbiological test results for all children (1 month old to 18 years old) who were admitted to our pediatric hospital between January 2000 to June 2015 and had positive clinical specimen cultures for P...
August 2, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28776045/whole-genome-sequencing-of-human-clinical-klebsiella-pneumoniae-isolates-reveals-misidentification-and-misunderstandings-of-klebsiella-pneumoniae-klebsiella-variicola-and-klebsiella-quasipneumoniae
#16
S Wesley Long, Sarah E Linson, Matthew Ojeda Saavedra, Concepcion Cantu, James J Davis, Thomas Brettin, Randall J Olsen
Klebsiella pneumoniae is a major threat to public health, causing significant morbidity and mortality worldwide. The emergence of highly drug-resistant strains is particularly concerning. There has been a recognition and division of Klebsiella pneumoniae into three distinct phylogenetic groups: Klebsiella pneumoniae, Klebsiella variicola, and Klebsiella quasipneumoniae. K. variicola and K. quasipneumoniae have often been described as opportunistic pathogens that have less virulence in humans than K. pneumoniae does...
July 2017: MSphere
https://www.readbyqxmd.com/read/28763660/vancomycin-resistant-enterococci-with-vana-gene-in-treated-municipal-wastewater-and-their-association-with-human-hospital-strains
#17
Veronika Oravcova, Matus Mihalcin, Jana Zakova, Lucie Pospisilova, Martina Masarikova, Ivan Literak
Vancomycin-resistant enterococci (VRE) are pathogens of increasing medical importance. In Brno, Czech Republic, we collected 37 samples from the effluent of a wastewater treatment plant (WWTP), 21 surface swabs from hospital settings, and 59 fecal samples from hospitalized patients and staff. Moreover, we collected 284 gull cloacal swabs from the colony situated 35km downstream the WWTP. Samples were cultured selectively. Enterococci were identified using MALDI-TOF MS, phenotypically tested for susceptibility to antibiotics, and by PCR for occurrence of resistance and virulence genes...
December 31, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28757016/characterization-of-corynebacterium-diphtheriae-c-confusum-and-c-amycolatum-isolated-from-sows-with-genitourinary-infection
#18
André P Poor, Luisa Z Moreno, Carlos E C Matajira, Beatriz M Parra, Vasco T M Gomes, Ana Paula S Silva, Mauricio C Dutra, Ana Paula G Christ, Mikaela R F Barbosa, Maria Inês Z Sato, Andrea M Moreno
Porcine Corynebacterium infection is still poorly studied, even though the pig has been described as an asymptomatic carrier of Corynebacterium species, including the zoonotic species C. ulcerans, C. confusum and C. amycolatum. Here we present the identification, molecular and antimicrobial susceptibility characterization of coryneform bacteria isolated from sows with urinary tract infection. C. diphtheriae, C. confusum and C. amycolatum were isolated from sows with urinary infection and metritis. Corynebacterium species were identified by MALDI-TOF MS and confirmed by 16S rRNA and rpoB sequencing...
August 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28747897/identification-of-emerging-human-mastitis-pathogens-by-maldi-tof-and-assessment-of-their-antibiotic-resistance-patterns
#19
María Marín, Rebeca Arroyo, Irene Espinosa-Martos, Leónides Fernández, Juan M Rodríguez
Lactational mastitis constitutes one of the main causes of undesired weaning, depriving the mother-infant pair from the benefits of breastfeeding; therefore, this condition should be considered a relevant public health issue. The role of specific microorganisms remains unclear since human milk cultures and antibiotic susceptibility testing (AST) are not routinely performed, despite the fact that this would be key to ensure an early and effective diagnosis and treatment. The objective of this study was to describe the culturable microbial diversity in 647 milk samples from breastfeeding women with clinical symptoms of mastitis by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) VITEK MS technology and to analyze the antimicrobial susceptibility profiles of a collection of isolates from these samples by the VITEK 2 AST system...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28743946/identification-of-the-eskape-pathogens-by-mass-spectrometric-analysis-of-microbial-membrane-glycolipids
#20
Lisa M Leung, William E Fondrie, Yohei Doi, J Kristie Johnson, Dudley K Strickland, Robert K Ernst, David R Goodlett
Rapid diagnostics that enable identification of infectious agents improve patient outcomes, antimicrobial stewardship, and length of hospital stay. Current methods for pathogen detection in the clinical laboratory include biological culture, nucleic acid amplification, ribosomal protein characterization, and genome sequencing. Pathogen identification from single colonies by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of high abundance proteins is gaining popularity in clinical laboratories...
July 25, 2017: Scientific Reports
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