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MALDI resistance

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https://www.readbyqxmd.com/read/29777151/integrated-proteomics-genomics-metabolomics-approaches-reveal-oxalic-acid-as-pathogenicity-factor-in-tilletia-indica-inciting-karnal-bunt-disease-of-wheat
#1
Vishakha Pandey, Manoj Singh, Dinesh Pandey, Anil Kumar
Tilletia indica incites Karnal bunt (KB) disease in wheat. To date, no KB resistant wheat cultivar could be developed due to non-availability of potential biomarkers related to pathogenicity/virulence for screening of resistant wheat genotypes. The present study was carried out to compare the proteomes of T. indica highly (TiK) and low (TiP) virulent isolates. Twenty one protein spots consistently observed as up-regulated/differential in the TiK proteome were selected for identification by MALDI-TOF/TOF. Identified sequences showed homology with fungal proteins playing essential role in plant infection and pathogen survival, including stress response, adhesion, fungal penetration, invasion, colonization, degradation of host cell wall, signal transduction pathway...
May 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29776363/rapid-detection-of-carbapenemase-activity-of-enterobacteriaceae-isolated-from-positive-blood-cultures-by-maldi-tof-ms
#2
Jiajia Yu, Jingxian Liu, Yuanrui Li, Jing Yu, Weinan Zhu, Ying Liu, Lisong Shen
BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been proved to be a useful tool for identification of pathogens directly isolated from blood cultures in clinical microbiology laboratories. β-Lactam antibiotics are commonly used for treatment of bloodstream infections caused by Enterobacteriaceae strains, and carbapenem is the superlative class of β-lactam antibiotics. Since the carbapenem resistance rate of Enterobacteriaceae strains raised year by year, efficient detection of carbapenemase activity and timely delivery of carbapenem susceptibility reports of Enterobacteriaceae strains isolated from blood cultures is important for clinicians...
May 18, 2018: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/29764754/detection-of-methicillin-resistant-coagulase-negative-staphylococci-harboring-the-class-a-mec-complex-by-maldi-tof-mass-spectrometry
#3
Dominik Schuster, Michaele Josten, Kathrin Janssen, Isabel Bodenstein, Cathrin Albert, Anna Schallenberg, Mike Gajdiss, Esther Sib, Christiane Szekat, Katja Kehl, Marijo Parčina, Gunnar T R Hischebeth, Gabriele Bierbaum
The aim of this study was to test the identification of methicillin resistance in coagulase-negative staphylococci by routine matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS). SCCmec cassettes of type II, III and VIII encode a small peptide called PSM-mec in the vicinity of mecA. It is visible at m/z 2415 during MALDI-TOF MS of whole cells of Staphylococcus aureus. In view of the fact that psm-mec has been identified in methicillin-resistant coagulase-negative staphylococci, we evaluated a collection of clinical coagulase-negative staphylococci, that contained 77...
May 8, 2018: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29758020/defining-icr-mo-an-intrinsic-colistin-resistance-determinant-from-moraxella-osloensis
#4
Wenhui Wei, Swaminath Srinivas, Jingxia Lin, Zichen Tang, Shihua Wang, Saif Ullah, Vishnu Goutham Kota, Youjun Feng
Polymyxin is the last line of defense against severe infections caused by carbapenem-resistant gram-negative pathogens. The emergence of transferable MCR-1/2 polymyxin resistance greatly challenges the renewed interest in colistin (polymyxin E) for clinical treatments. Recent studies have suggested that Moraxella species are a putative reservoir for MCR-1/2 genetic determinants. Here, we report the functional definition of ICR-Mo from M. osloensis, a chromosomally encoded determinant of colistin resistance, in close relation to current MCR-1/2 family...
May 14, 2018: PLoS Genetics
https://www.readbyqxmd.com/read/29729017/risk-assessment-for-the-spread-of-candida-sp-in-dental-chair-unit-waterlines-using-molecular-techniques
#5
Wissame Mazari, Zahia Boucherit-Otmani, Imad Abdelhamid El Haci, Amine Ilahi, Kebir Boucherit
AIMS: The aim of this study was to evaluate the presence of yeasts in dental chair unit waterlines (DCUWLs) and to test their ability to form biofilms. MATERIALS AND METHODS: Eighteen dental waterlines were analysed by culture in liquid Sabouraud in order to allow the quantification and the purification of isolated yeasts from their internal surfaces. All isolates were identified by standard laboratory procedures, including CHROMagar Candida medium for orientation, commercial yeast identification system Api Candida, MALDI-TOF MS and DNA sequencing...
May 4, 2018: International Dental Journal
https://www.readbyqxmd.com/read/29720352/bacteremia-and-meningitis-caused-by-oxa-23-producing-acinetobacter-baumannii-molecular-characterization-and-susceptibility-testing-for-alternative-antibiotics
#6
Kamile Francine Schuertz, Felipe Francisco Tuon, Jussara Kasuko Palmeiro, Danieli Conte, João Paulo Marochi Telles, Lucas Eduardo Trevisoli, Libera Maria Dalla-Costa
BACKGROUND: Carbapenem-resistant Acinetobacter baumannii infection is a concern in developing countries due to high incidence, few therapeutic options, and increasing costs. OBJECTIVE: Characterize and analyze the antibiotic susceptibility patterns of carbapenem-resistant A. baumannii isolates and evaluate clinical data of meningitis and bacteremia caused by this microorganism. METHODS: Twenty-six A. baumannii isolates from 23 patients were identified by MALDI-TOF and automated methods and genotyped using pulsed field genotyping electrophoresis...
April 24, 2018: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/29704942/identification-of-staphylococcus-epidermidis-with-transferrable-mupirocin-resistance-from-canine-skin
#7
C C Rossi, B A B Salgado, E M Barros, P A de Campos Braga, M N Eberlin, W Lilenbaum, M Giambiagi-deMarval
Resistance to mupirocin was analysed in Staphylococcus spp. isolated from healthy dogs (n=21) and dogs with pyoderma (n=47) or otitis externa (n=52). Isolates were identified to species level by MALDI-TOF and PCR-RFLP of the groEL gene. One isolate of Staphylococcus epidermidis from the skin of a healthy dog, which harboured a plasmid carrying the mupA gene, was resistant to mupirocin.
May 2018: Veterinary Journal
https://www.readbyqxmd.com/read/29695525/ultra-high-resolution-mass-spectrometry-of-closely-related-dermatophytes-with-different-clinical-predilections
#8
Karolina Dukik, Joanna Freeke, Azadeh Jamalian, Bert Gerrits van den Ende, Ping Yip, James L Stephenson, G Sybren de Hoog, J Benjamin Stielow
In the present study, an innovative top-down liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the identification of clinically relevant fungi is tested using a model set of dermatophyte strains. The methodology characterizes intact proteins derived from Trichophyton species which are used as parameters of differentiation. To test its resolving power compared to that of traditional Sanger sequencing and matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF), twenty-four strains of closely related dermatophytes, Trichophyton rubrum, T...
April 25, 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29678480/gram-negative-anaerobe-susceptibility-testing-in-clinical-isolates-using-sensititre-and-etest-methods
#9
C Hughes, C Ashhurst-Smith, J K Ferguson
Gram negative anaerobic organisms are important pathogens in a range of clinical infections, and susceptibility testing is not commonly performed in the microbiology laboratory. We performed anaerobic susceptibility testing on 70 clinically relevant Gram negative anaerobes isolated from routine cultures in a busy diagnostic laboratory which were identified by MALDI-TOF mass spectrometry (MALDI-TOF MS). The susceptibility testing was performed by two methods: Sensititre trays (ThermoFisher Scientific) against 15 different antibiotics, and Etests (bioMérieux) against five clinically relevant antibiotics (metronidazole, piperacillin-tazobactam, amoxicillin-clavulanate, meropenem and clindamycin)...
April 17, 2018: Pathology
https://www.readbyqxmd.com/read/29675779/antibiotic-resistance-and-molecular-characteristics-of-staphylococcus-aureus-isolated-from-backyard-raised-pigs-and-pig-workers
#10
Asabe Halimat Momoh, Jacob K P Kwaga, Mohammed Bello, Anthony K B Sackey, Anders Rhod Larsen
Staphylococcus aureus is a commensal and pathogenic bacterium with impact on public health and livestock industry. The study investigated nasal carriage, antibiotic resistance, and molecular characterization of S. aureus in pigs and pig workers. Nasal swabs from 300 backyard-raised pigs and 101 pig workers were used for the study. Resulting isolates were confirmed using MALDI-TOF MS, tested for antibiotic resistance, and three different multiplex PCRs were used to detect enterotoxin, mecA, spaA, scn, and pvl genes...
April 19, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29674058/a-novel-protein-digestion-method-with-the-assistance-of-alternating-current-denaturation-for-high-efficient-protein-digestion-and-mass-spectrometry-analysis
#11
Xiaoxun Han, Yiming Xie, Qin Wu, Shuaibin Wu
Protein denaturation has always displayed a huge necessity for mass spectrometry (MS)-based protein identification methods in proteomics. In this research, a novel protein digestion method with the assistance of alternating current (AC) denaturation has been proposed and evaluated. In this method, merely, 200 mM ammonium bicarbonate buffer solution (pH, 8.2) was used to dissolve proteins and act as the electrolyte, and protein denaturation could be achieved in several seconds. For apo-transferrin, ovalbumin and bovine serum albumin that are resistant to digestion in their native states, confident amino acid sequence coverage by matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis were obtained after 200 v AC denaturation...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29663868/imaging-mass-spectrometry-of-endogenous-polypeptides-and-secondary-metabolites-from-galls-induced-by-root-knot-nematodes-in-tomato-roots
#12
Eder Alves Barbosa, Mauro Ferreira Bonfim Junior, Carlos Bloch, Thales Lima Rocha, Gilbert Engler, Janice de Almeida Engler
Nematodes are devastating pests that infect most cultivated plant species and cause considerable agricultural losses worldwide. The understanding of metabolic adjustments induced during plant-nematode interaction is crucial to generate resistant plants or to select more efficient molecules to fight against this pest. Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been used herein for in situ detection and mapping endogenous polypeptides and secondary metabolites from nematode-induced gall tissue...
April 17, 2018: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/29648612/analysis-of-escherichia-coli-sts-and-resistance-mechanisms-in-sewage-from-islamabad-pakistan-indicates-a-difference-in-e-coli-carriage-types-between-south-asia-and-europe
#13
Rabaab Zahra, Saba Javeed, Bibi Malala, Dmitriy Babenko, Mark A Toleman
Objectives: To discover the Escherichia coli STs and associated resistance mechanisms in the community in Islamabad, Pakistan by analysis of E. coli isolates in sewage. Methods: One hundred and ten E. coli were isolated from sewage across the city of Islamabad without antibiotic bias and confirmed as E. coli by MALDI-TOF MS. Isolates were characterized by fumC/fimH (CH) typing and core-genome MLST. Resistance mechanisms, virulence genes, phylotypes and plasmid incompatibility types were determined in a subset of isolates by in silico analysis...
April 10, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29642826/-investigation-of-high-risk-st131-clone-in-esbl-producing-escherichia-coli-isolates-isolated-from-urine-and-non-urinary-clinical-specimens-with-maldi-tof-ms-and-real-time-pcr
#14
Zeynep Çizmeci, Barış Otlu, Elif Aktaş, Seyhan Ördekçi, Özlem Açıkgöz, Nuray Güleç
In recent years, the ST131 clone was identified as a high risk pandemic clone among Escherichia coli isolates by multilocus sequence typing (MLST) studies and has been associated with extended spectrum beta-lactamase (ESBL) production (often with CTX-M-15) and antibiotic resistance especially against fluoroquinolones. The aim of this study was to determine the rate of high risk ST131 clone in ESBL producing E.coli isolates in our region, to investigate the sensitivity of MALDI-TOF MS in the detection of ST131 clone, and to compare the frequency of antimicrobial resistance among ST131 and non-ST131 isolates...
January 2018: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29628921/prevalence-and-characterization-of-multi-drug-resistant-gram-negative-bacilli-isolated-from-lebanese-poultry-a-nationwide-study
#15
Iman Dandachi, Elie S Sokhn, Elias A Dahdouh, Eid Azar, Bassel El-Bazzal, Jean-Marc Rolain, Ziad Daoud
Currently, antimicrobial resistance is one of the most prominent public health issues. In fact, there is increasing evidence that animals constitute a reservoir of antimicrobial resistance. In collaboration with the Lebanese Ministry of Agriculture, the aim of this study was to determine the prevalence of intestinal carriage of multi-drug-resistant Gram-negative Bacilli in poultry farms at the national level. Between August and December 2015, 981 fecal swabs were obtained from 49 poultry farms distributed across Lebanon...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29626757/nmta-a-novel-metallothionein-of-anabaena-sp-strain-pcc-7120-imparts-protection-against-cadmium-stress-but-not-oxidative-stress
#16
Divya T V, Pallavi Chandwadkar, Celin Acharya
Metallothioneins (MTs) are low molecular weight, sulfhydryl-containing, cysteine-rich, metal-binding proteins. Eukaryotes have multiple metallothionein genes; however, there is dearth of reports on prokaryotic metallothioneins. Bacterial MTs with SmtA from Synechococcus PCC 7942 as prototype have been studied in the context of cadmium detoxification. In this study, a smtA related ORF, namely nmtA, was identified in the heterocystous, nitrogen-fixing cyanobacterium, Anabaena PCC 7120. A recombinant N-terminal histidine-tagged Anabaena NmtA protein was overexpressed in Escherichia coli and purified...
March 30, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29626622/bacteroides-fragilis-a-whole-maldi-based-workflow-from-identification-to-confirmation-of-carbapenemase-production-for-routine-laboratories
#17
M Cordovana, M Kostrzewa, J Sóki, E Witt, S Ambretti, A B Pranada
Bacteroides fragilis is a frequent anaerobic pathogen and can cause severe infections. Resistance to carbapenems, associated with the cfiA gene encoded carbapenemase, represents an emerging problem. To date, no rapid methods are available to detect and confirm this resistance mechanism in routine laboratories, and the missed recognition of carbapenemase-producing strains can lead to therapeutic failures. In this study we have investigated a whole MALDI-TOF MS-based workflow to detect carbapenemase-producing B...
April 4, 2018: Anaerobe
https://www.readbyqxmd.com/read/29621582/fast-and-easy-detection-of-cmy-2-in-escherichia-coli-by-direct-maldi-tof-mass-spectrometry
#18
Roque Figueroa Espinosa, Valeria Rumi, Martín Marchisio, Daniela Cejas, Marcela Radice, Carlos Vay, Rubén Barrios, Gabriel Gutkind, José Di Conza
Fast typing methods for third generation cephalosporin resistance mechanisms are needed to guide appropriate treatment and prevent potential dissemination events. In this study we used a novel short and fast methodology for the identification of CMY-2 in 50 well characterized clinical isolates of E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry- MALDI-TOF MS. Samples were prepared using the double layer sinapinic acid technique for detection of intact proteins Comparison among mass spectral profile of different strains between m/z 35,000-45,000 Da showed that two groups of isolates could be differentiated after peak analysis...
April 2, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29617332/rapid-identification-of-intact-staphylococcal-bacteriophages-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#19
Dana Štveráková, Ondrej Šedo, Martin Benešík, Zbyněk Zdráhal, Jiří Doškař, Roman Pantůček
Staphylococcus aureus is a major causative agent of infections associated with hospital environments, where antibiotic-resistant strains have emerged as a significant threat. Phage therapy could offer a safe and effective alternative to antibiotics. Phage preparations should comply with quality and safety requirements; therefore, it is important to develop efficient production control technologies. This study was conducted to develop and evaluate a rapid and reliable method for identifying staphylococcal bacteriophages, based on detecting their specific proteins using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) profiling that is among the suggested methods for meeting the regulations of pharmaceutical authorities...
April 4, 2018: Viruses
https://www.readbyqxmd.com/read/29608117/clinical-impact-of-rapid-bacterial-identification-by-maldi-tof-ms-combined-with-the-b%C3%A3%C2%AAta-lacta%C3%A2-test-on-early-antibiotic-adaptation-by-an-antimicrobial-stewardship-team-in-bloodstream-infections
#20
A Mizrahi, J Amzalag, C Couzigou, G Péan De Ponfilly, B Pilmis, A Le Monnier
BACKGROUND: Bloodstream infections (BSI) can potentially be life-threatening infections and are associated with a high crude mortality, moreover with an inappropriate first-line antibiotic therapy. Bacterial resistance is more and more frequently observed. New strategies of BSI management are urgently needed. MATERIALS AND METHODS: During an 18-months period, we prospectively evaluated the clinical impact of rapid bacterial identification by MALDI-TOF MS technology combined with an antimicrobial stewardship team (AST) intervention...
April 2, 2018: Infectious Diseases
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