keyword
MENU ▼
Read by QxMD icon Read
search

Myocyte

keyword
https://www.readbyqxmd.com/read/28747891/proliferative-role-of-kv11-channels-in-murine-arteries
#1
Vincenzo Barrese, Pilar Cidad, Shuk Y Yeung, Jose R López-López, Alister J McNeish, Susumu Ohya, Maria T Pérez-García, Iain A Greenwood
K(+) channels encoded by the ether-a-go-go related gene (ERG1 or KCNH2) are important determinants of the cardiac action potential. Expression of both cardiac isoforms (ERG1a and ERG1b) were identified in murine portal vein and distinctive voltage-gated K(+) currents were recorded from single myocytes. The aim of the present study was to ascertain the expression and functional impact of ERG channels in murine arteries. Methods: Quantitative RT-PCR was undertaken on RNA extracted from a number of murine arteries...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28746924/cyclin-dependent-kinase-inhibitor-p21waf1-cip1-facilitates-the-development-of-cardiac-hypertrophy
#2
Yang-Fei Tong, Yan Wang, Yuan-Yuan Ding, Jing-Mei Li, Xi-Chun Pan, Xiao-Lan Lu, Xiao-Hong Chen, Ya Liu, Hai-Gang Zhang
BACKGROUND/AIMS: Adult cardiomyocytes can re-enter cell cycle as stimulated by prohypertrophic factors although they withdraw from cell cycle soon after birth. p21WAF1/CIP1, a cyclin-dependent kinase inhibitor, has been implicated in cardiac hypertrophy, however, its precise contribution to this process remains largely unclear. METHODS: The gene expression profile in left ventricle (LV) of spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats was determined using quantitative PCR array and verified by real-time PCR and Western blotting...
July 24, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28743804/paracrine-effects-of-the-pluripotent-stem-cell-derived-cardiac-myocytes-salvage-the-injured-myocardium
#3
Atsushi Tachibana, Michelle R Santoso, Morteza Mahmoudi, Praveen Shukla, Lei Wang, Mihoko Bennett, Andrew B Goldstone, Mouer Wang, Masahiro Fukushi, Antje Ebert, Y Joseph Woo, Eric Rulifson, Phillip C Yang
Rationale: Cardiac myocytes derived from pluripotent stem cells have demonstrated the potential to mitigate damage of the infarcted myocardium and improve left ventricular ejection fraction (LVEF). However, the mechanism underlying the functional benefit is unclear. Objective: To evaluate whether the transplantation of cardiac lineage differentiated derivatives enhance myocardial viability and restore LVEF more effectively than undifferentiated pluripotent stem cells after a myocardial injury (MI). Herein, we utilize novel multimodality evaluation of human embryonic stem cells (hESCs), hESC-derived cardiac myocytes (hCMs), human induced pluripotent stem cells (iPSCs), and iPSC-derived cardiac myocytes (iCMs) in a murine MI model...
July 25, 2017: Circulation Research
https://www.readbyqxmd.com/read/28743763/a-key-role-for-peroxynitrite-mediated-inhibition-of-cardiac-erg-kv11-1-k-channels-in-carbon-monoxide-induced-proarrhythmic-early-afterdepolarizations
#4
Moza M Al-Owais, Nishani T Hettiarachchi, Hannah M Kirton, Matthew E Hardy, John P Boyle, Jason L Scragg, Derek S Steele, Chris Peers
Exposure to CO causes early afterdepolarization arrhythmias. Previous studies in rats have indicated that arrhythmias arose as a result of augmentation of the late Na(+) current. The purpose of the present study was to examine the basis for CO-induced arrhythmias in guinea pig myocytes in which action potentials (APs) more closely resemble those of human myocytes. Whole-cell current- and voltage-clamp recordings were made from isolated guinea pig myocytes as well as from human embryonic kidney 293 (HEK293) cells that express wild-type or a C723S mutant form of ether-a-go-go-related gene (ERG; Kv11...
July 25, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28743749/sumoylation-determines-the-voltage-required-to-activate-cardiac-iks-channels
#5
Dazhi Xiong, Tian Li, Hui Dai, Anthony F Arena, Leigh D Plant, Steve A N Goldstein
IKs channels open in response to depolarization of the membrane voltage during the cardiac action potential, passing potassium ions outward to repolarize ventricular myocytes and end each beat. Here, we show that the voltage required to activate IKs channels depends on their covalent modification by small ubiquitin-like modifier (SUMO) proteins. IKs channels are comprised of four KCNQ1 pore-forming subunits, two KCNE1 accessory subunits, and up to four SUMOs, one on Lys424 of each KCNQ1 subunit. Each SUMO shifts the half-maximal activation voltage (V1/2) of IKs ∼ +8 mV, producing a maximal +34-mV shift in neonatal mouse cardiac myocytes or Chinese hamster ovary (CHO) cells expressing the mouse or human subunits...
July 25, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28741800/eteplirsen-for-the-treatment-of-duchenne-muscular-dystrophy-quality-of-evidence-concerns-an-alternative-viewpoint
#6
Adriane N Irwin, Megan C Herink
We read the paper by Reinig AM and colleagues entitled "Advances in the Treatment of Duchenne Muscular Dystrophy: New and Emerging Pharmacotherapies" with great interest.(1) The authors discuss several novel therapies to increase production of dystrophin in myocytes, of which, eteplirsen (Exondys) is the only currently approved by the US Food & Drug Administration (FDA).(2) Pharmacologic management of Duchenne muscular dystrophy (DMD) centers on the use of glucocorticoids which works by decreasing inflammation and reducing immune system activity...
July 25, 2017: Pharmacotherapy
https://www.readbyqxmd.com/read/28739174/inhibition-of-mitochondrial-fission-prevents-hypoxia-induced-metabolic-shift-and-cellular-proliferation-of-pulmonary-arterial-smooth-muscle-cells
#7
Valentina Parra, Roberto Bravo-Sagua, Ignacio Norambuena-Soto, Carolina P Hernández-Fuentes, Andrés G Gómez-Contreras, Hugo E Verdejo, Rosemarie Mellado, Mario Chiong, Sergio Lavandero, Pablo F Castro
Inhibition of mitochondrial fission prevents hypoxia-induced metabolic shift and cellular proliferation of pulmonary arterial smooth muscle cells. Valentina Parra, Roberto Bravo-Sagua, Ignacio Norambuena-Soto, Carolina P. Hernández-Fuentes, Andrés G. Gómez-Contreras, Hugo E. Verdejo, Rosemarie Mellado, Mario Chiong, Sergio Lavandero, Pablo F. Castro. Chronic hypoxia exacerbates proliferation of pulmonary arterial smooth muscle cells (PASMC), thereby reducing the lumen of pulmonary arteries. This leads to poor blood oxygenation and cardiac work overload, which are the basis of diseases such as pulmonary artery hypertension (PAH)...
July 21, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28736364/-angiotensin-1-7-protects-cardiac-myocytes-against-high-glucose-induced-injury-by-inhibiting-clc-3-chloride-channels
#8
Shao-Ai Ccai, Jing-Fu Chen, Mei-Ji Chen, Jian-Cong Lin, Jian-Qiang Feng, Kai Lin, Xi-Mei Zhi, Wei-Jie Zhang, Wen Wu
OBJECTIVE: To explore whether angiotensin-(1-7) [Ang-(1-7)] protects cardiac myocytes against high glucose (HG)-induced injury by inhibiting ClC-3 chloride channels. METHOD: H9c2 cardiac cells were exposed to 35 mmol/L glucose for 24 h to establish a cell injury model. The cells were treated with Ang-(1-7) or the inhibitor of chloride channel (NPPB) in the presence of HG for 24 h to observe the changes in HG-induced cell injury. Cell counter kit 8 (CCK-8) assay was used to test the cell viability, and the morphological changes of the apoptotic cells were detected using Hoechst 33258 staining and fluorescent microscopy...
July 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28733449/mitochondrial-function-in-engineered-cardiac-tissues-is-co-regulated-by-extracellular-matrix-elasticity-and-tissue-alignment
#9
Davi Marco Lyra-Leite, Allen Mariano Andres, Andrew Patrick Petersen, Nethika Ruvini Ariyasinghe, Nathan Cho, Jezell Athena Lee, Roberta A Gottlieb, Megan L McCain
Mitochondria in cardiac myocytes are critical for generating ATP to meet the high metabolic demands associated with sarcomere shortening. Distinct remodeling of mitochondrial structure and function occur in cardiac myocytes in both developmental and pathological settings. However, the factors that underlie these changes are poorly understood. Because remodeling of tissue architecture and extracellular matrix (ECM) elasticity are also hallmarks of ventricular development and disease, we hypothesize that these environmental factors regulate mitochondrial function in cardiac myocytes...
July 21, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/28733351/multi-cellular-transcriptional-analysis-of-mammalian-heart-regeneration
#10
Gregory A Quaife-Ryan, Choon Boon Sim, Mark Ziemann, Antony Kaspi, Haloom Rafehi, Mirana Ramialison, Assam El-Osta, James E Hudson, Enzo R Porrello
Background -The inability of the adult mammalian heart to regenerate following injury represents a major barrier in cardiovascular medicine. In contrast, the neonatal mammalian heart retains a transient capacity for regeneration, which is lost shortly after birth. Defining the molecular mechanisms that govern regenerative capacity in the neonatal period remains a central goal in cardiac biology. Here, we assemble a transcriptomic framework of multiple cardiac cell populations during postnatal development and following injury, which enables comparative analyses of the regenerative (neonatal) versus non-regenerative (adult) state for the first time...
July 21, 2017: Circulation
https://www.readbyqxmd.com/read/28730832/liraglutide-preserves-intracellular-calcium-handling-in-isolated-murine-myocytes-exposed-to-oxidative-stress
#11
S Palee, S C Chattipakorn, N Chattipakorn
In ischemic/reperfusion (I/R) injured hearts, severe oxidative stress occurs and is associated with intracellular calcium (Ca(2+)) overload. Glucagon-Like Peptide 1 (GLP-1) analogues have been shown to exert cardioprotection in I/R heart. However, there is little information regarding the effects of GLP-1 analogue on the intracellular Ca(2+) regulation in the presence of oxidative stress. Therefore, we investigated the effects of GLP-1 analogue, (liraglutide, 10 microM) applied before or after hydrogen peroxide (H(2)O(2), 50 microM) treatment on intracellular Ca(2+) regulation in isolated cardiomyocytes...
July 18, 2017: Physiological Research
https://www.readbyqxmd.com/read/28729685/nucleocytoplasmic-shuttling-of-histone-deacetylase-9-controls-activity-dependent-thalamocortical-axon-branching
#12
Ricardo Alchini, Haruka Sato, Naoyuki Matsumoto, Tomomi Shimogori, Noriyuki Sugo, Nobuhiko Yamamoto
During development, thalamocortical (TC) axons form branches in an activity-dependent fashion. Here we investigated how neuronal activity is converted to molecular signals, focusing on an epigenetic mechanism involving histone deacetylases (HDACs). Immunohistochemistry demonstrated that HDAC9 was translocated from the nucleus to the cytoplasm of thalamic cells during the first postnatal week in rats. In organotypic co-cultures of the thalamus and cortex, fluorescent protein-tagged HDAC9 also exhibited nuclueocytoplasmic translocation in thalamic cells during culturing, which was reversed by tetrodotoxin treatment...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729454/multimodal-regulation-of-cardiac-myocyte-proliferation
#13
REVIEW
Xuejun Yuan, Thomas Braun
Efficient cardiac regeneration is closely associated with the ability of cardiac myocytes to proliferate. Fetal or neonatal mouse hearts containing proliferating cardiac myocytes regenerate even extensive injuries, whereas adult hearts containing mostly post-mitotic cardiac myocytes have lost this ability. The same correlation is seen in some homoiotherm species such as teleost fish and urodelian amphibians leading to the hypothesis that cardiac myocyte proliferation is a major driver of heart regeneration...
July 21, 2017: Circulation Research
https://www.readbyqxmd.com/read/28728400/studies-on-meat-color-myoglobin-content-enzyme-activities-and-genes-associated-with-oxidative-potential-of-pigs-slaughtered-at-different-growth-stages
#14
Qin Ping Yu, Ding Yuan Feng, Juan Xiao, Fan Wu, Xiao Jun He, Min Hao Xia, Tao Dong, Yi Hua Liu, Hui Ze Tan, Shi Geng Zou, Tao Zheng, Xian Hua Ou, Jian Jun Zuo
Objective: This experiment was conducted to investigate meat color, myoglobin (Mb) content, enzyme activities, and expression of genes associated with oxidative potential of pigs slaughtered at different growth stages. Methods: Sixty 4-week-old Duroc × Landrace × Yorkshire pigs were assigned to 6 replicate groups, each containing 10 pigs. One pig from each group was sacrificed at day 35, 63, 98, and 161 to isolate longissimus dorsi and triceps muscles. Results: Meat color scores were higher in pigs at 35 d than those at 63 d and 98 d (P < 0...
May 14, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28728382/effects-of-a-traditional-chinese-medicine-formula-and-its-extraction-on-muscle-fiber-characteristics-in-finishing-pigs-porcine-cell-proliferation-and-isoforms-of-myosin-heavy-chain-gene-expression-in-myocytes
#15
Qin Ping Yu, Ding Yuan Feng, Xiao Jun He, Fan Wu, Min Hao Xia, Tao Dong, Yi Hua Liu, Hui Ze Tan, Shi Geng Zou, Tao Zheng, Xian Hua Ou, Jian Jun Zou
Objective: This study evaluated the effects of a traditional Chinese medicine formula (TCMF) on muscle fiber characteristics in finishing pigs and the effects of the formula's extract (distilled water, ethyl acetate and petroleum ether extraction) on porcine cell proliferation and isoforms of MyHC gene expression in myocytes. Methods: XThe psoas major muscle was obtained from pigs at the end of the experiment. Muscle fiber characteristics in the psoas major muscle were analyzed using myosin ATPase staining...
June 26, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28726981/triennial-growth-and-development-symposium-factors-influencing-bovine-intramuscular-adipose-tissue-development-and-cellularity
#16
E Albrecht, L Schering, Y Liu, K Komolka, C Kühn, K Wimmers, T Gotoh, S Maak
Appearance, distribution, and amount of intramuscular fat (IMF), often referred to as marbling, are highly variable and depend on environmental and genetic factors. On the molecular level, the concerted action of several drivers, including hormones, receptors, transcription factors, etc., determines where clusters of adipocytes arise. Therefore, the aim of future studies remains to identify such factors as biological markers of IMF to increase the ability to identify animals that deposit IMF early in age to increase efficiency of high-quality meat production...
May 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28726917/engineering-micromyocardium-to-delineate-cellular-and-extracellular-regulation-of-myocardial-tissue-contractility
#17
Nethika R Ariyasinghe, Caitlin H Reck, Alyssa A Viscio, Andrew P Petersen, Davi M Lyra-Leite, Nathan Cho, Megan L McCain
Cardiovascular diseases are a leading cause of death, in part due to limitations of existing models of the myocardium. Myocardium consists of aligned, contractile cardiac myocytes interspersed with fibroblasts that synthesize extracellular matrix (ECM). The cellular demographics and biochemical and mechanical properties of the ECM remodel in many different cardiac diseases. However, the impact of diverse cellular and extracellular remodeling on the contractile output of the myocardium are poorly understood...
July 20, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28726208/immunolocation-of-heme-oxygenases-in-the-walls-of-cerebral-arteries-of-various-diameters-in-rats
#18
V M Chertok, A E Kotsyuba
The distribution of two enzymes involved in the formation of carbon monoxide, heme oxygenases 1 and 2, in the pial branches of orders I-V of the middle cerebral artery basin and in intracerebral vessels was studied in adult Wistar rats. Immunohistochemical studies detected hemeoxygenase-2 in the endothelium of the small pial and intracerebral arterioles and in myocytes of pial branches I-III. Heme oxygenase 1, an inducible form of the enzyme, is normally not expressed in the cerebral vessels, but the enzyme is expressed in response to sodium metaarsenite...
June 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28725491/ds16570511-is-a-small-molecule-inhibitor-of-the-mitochondrial-calcium-uniporter
#19
Naohiro Kon, Michiko Murakoshi, Aya Isobe, Katsuji Kagechika, Naoki Miyoshi, Takahiro Nagayama
In cardiac myocytes, regulation of mitochondrial Ca(2+) is important for cellular signaling and cardiac contraction. Ca(2+) entry into the mitochondria is mediated by a highly selective Ca(2+) channel called the mitochondrial calcium uniporter, which consists of a pore-forming subunit MCU and regulatory subunits such as MICU1. Although pharmacological regulation of the mitochondrial Ca(2+) influx is a promising approach to controlling the cellular functions, a cell-permeable and specific inhibitor of the mitochondrial calcium uniporter has not yet been developed...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28725037/bursting-dynamics-in-the-normal-and-failing-hearts
#20
Vladimir E Bondarenko, Andrey L Shilnikov
A failing heart differs from healthy hearts by an array of symptomatic characteristics, including impaired Ca(2+) transients, upregulation of Na(+)/Ca(2+) exchanger function, reduction of Ca(2+) uptake to sarcoplasmic reticulum, reduced K(+) currents, and increased propensity to arrhythmias. While significant efforts have been made in both experimental studies and model development to display the causes of heart failure, the full process of deterioration from a healthy to a failing heart yet remains deficiently understood...
July 19, 2017: Scientific Reports
keyword
keyword
4901
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"