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Canine T cells

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https://www.readbyqxmd.com/read/28317209/the-effects-of-cryopreservation-on-the-expression-of-canine-regulatory-t-cell-markers
#1
Noemi Tarpataki, Marcin Wawrzyniak, Cezmi A Akdis, Beate Rückert, Marina L Meli, Nina M Fischer, Claude Favrot, Ana Rostaher
BACKGROUND: Regulatory T (Treg) cells have been described as key regulators in various immunological processes and are of growing interest in veterinary allergy. Cryopreservation of immune cells is performed routinely in human basic science research and in clinical studies. As such, it allows batch testing of collected samples at a single time point, resulting in a significant reduction in sample variability. Data which describe the effects of cryopreservation on Treg cell frequency and functionality in the canine species are important to inform future research...
March 19, 2017: Veterinary Dermatology
https://www.readbyqxmd.com/read/28317140/comparison-of-the-effects-of-cefazolin-and-ceftriaxone-on-canine-chondrocyte-culture
#2
P Siengdee, W Pradit, T Euppayo, S Chomdej, K Nganvongpanit
Cephalosporins (CEFs) are antibiotics frequently used to treat bone infections and septic arthritis. The effects of CEFs on chondrocytes have not been studied until now. Cefazolin (cef1) and ceftriaxone (cef3), first-and third-generation CEFs, were selected to investigate their direct effects on normal and osteoarthritic (OA) primary canine chondrocytes, which were either nonstimulated or stimulated with the pro-inflammatory cytokine IL-1β. In our results, treatment with CEFs increased the negative effects on both conditioned normal and OA chondrocytes, especially when applied to IL-1β-stimulated cells (inflammatory stimulus)...
March 19, 2017: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28283079/molecular-investigation-of-the-direct-anti-tumour-effects-of-nonsteroidal-anti-inflammatory-drugs-in-a-panel-of-canine-cancer-cell-lines
#3
R Yoshitake, K Saeki, M Watanabe, N Nakaoka, S M Ong, M Hanafusa, N Choisunirachon, N Fujita, R Nishimura, T Nakagawa
Nonsteroidal anti-inflammatory drugs (NSAIDs) have been suggested as effective adjunctive anti-tumour agents in human and veterinary medicine. However, the molecular mechanisms associated with their anti-tumour effects and correlations with the expression of cyclooxygenase (COX) and related molecules in tumours remain controversial. The objective of this study was to compare the expression profiles of COX and related molecules with NSAID sensitivity and to explore the molecular mechanisms of anti-tumour effects...
March 2017: Veterinary Journal
https://www.readbyqxmd.com/read/28247754/small-sized-mpeg-plga-nanoparticles-of-schisantherin-a-with-sustained-release-for-enhanced-brain-uptake-and-anti-parkinsonian-activity
#4
Tongkai Chen, Chuwen Li, Ye Li, Xiang Yi, Ruibing Wang, Simon Ming-Yuen Lee, Ying Zheng
Schisantherin A (SA) is a promising anti-Parkinsonism natural product. However, its poor water solubility and rapid serum clearance impose significant barriers to delivery of SA to the brain. This work aimed to develop SA in a nanoparticle formulation that extended SA circulation in the bloodstream and consequently an increased brain uptake and thus to be potentially efficacious for the treatment of Parkinson's disease (PD). Spherical SA nanoparticles with a mean particle size of 70 nm were prepared by encapsulating SA into methoxy poly(ethylene glycol)-block-poly(d,l)-lactic-co-glycolic acid (mPEG-PLGA) nanoparticles (SA-NPs) with an encapsulation efficiency of ∼91% and drug loading of ∼28%...
March 9, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28242002/canine-peripheral-blood-cd4-cd8-double-positive-tcell-subpopulations-exhibit-distinct-tcell-phenotypes-and-effector-functions
#5
Kathrin Rothe, Doris Bismarck, Mathias Büttner, Gottfried Alber, Heiner von Buttlar
Canine peripheral blood CD4(+)CD8α(+) double-positive (dp) Tcells represent a small population of T lymphocytes that are not only derived from single-positive (sp) CD4(+) but also from CD8(+) Tcells. Dp Tcells can be subdivided in three different subpopulations according to their expression levels of CD4 and CD8α, i.e. the CD4(dim)CD8α(bright), CD4(bright)CD8α(bright), and the CD4(bright)CD8α(dim) subsets. Previously we could show that total canine peripheral CD4(+)CD8α(+) dp Tcells have features of activated Tcells...
March 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28207898/distinct-susceptibility-and-applicability-of-mdck-derivatives-for-influenza-virus-research
#6
Shih-Chao Lin, Matthew A Kappes, Mei-Chun Chen, Chi-Chen Lin, Tony T Wang
Madin-Darby Canine Kidney (MDCK) cells are widely utilized as a substrate for influenza virus isolation and propagation due to the high yields of virus. Here we compared the conventional MDCK cell line, MDCK-SIAT1 and MDCK-London for viral production, cell survival, and suitability in testing antivirals using six influenza strains including two H1N1 (pandemic and epidemic strains), three H3N2 and one influenza B strain. Overall our results suggest that MDCK-London cell line is superior for virus culturing and quantification, and hence an ideal platform to evaluate antiviral drug efficacy against multiple strains of influenza...
2017: PloS One
https://www.readbyqxmd.com/read/28181139/cytoprotective-and-anti-apoptotic-role-of-terminalia-arjuna-on-oxalate-injured-renal-epithelial-cells
#7
Amisha Mittal, Simran Tandon, Surender Kumar Singla, Chanderdeep Tandon
Urolithiasis is one of the painful multifactorial disorders caused by metabolic abnormalities influencing the composition of body fluids and urine. The bark of Terminalia arjuna (T. arjuna), very well known in Ayurveda for the treatment of cardiovascular diseases, possesses antioxidant and diuretic activity. The present study was undertaken to investigate the antiurolithiatic efficacy of aqueous extract of bark of T. arjuna on oxalate-induced injury to renal tubular epithelial cells. Madin-Darby canine kidney (MDCK) cells were exposed to 2 mM oxalate for 48 h to evaluate the protective effect of T...
February 8, 2017: Cytotechnology
https://www.readbyqxmd.com/read/28145161/repetitive-ischemia-increases-myocardial-dimethylarginine-dimethylaminohydrolase-1-expression
#8
Ping Zhang, John T Fassett, Guangshuo Zhu, Jingxin Li, Xinli Hu, Xin Xu, Yingjie Chen, Robert J Bache
Pharmacologic inhibition of nitric oxide production inhibits growth of coronary collateral vessels. Dimethylarginine dimethylaminohydrolase 1 (DDAH1) is the major enzyme that degrades asymmetric dimethylarginine (ADMA), a potent inhibitor of nitric oxide synthase. Here we examined regulation of the ADMA-DDAH1 pathway in a canine model of recurrent myocardial ischemia during the time when coronary collateral growth is known to occur. Under basal conditions, DDAH1 expression was non-uniform across the left ventricular (LV) wall, with expression strongest in the subepicardium...
January 1, 2017: Vascular Medicine
https://www.readbyqxmd.com/read/28140476/effects-of-leukoreduction-and-storage-on-erythrocyte-phosphatidylserine-expression-and-eicosanoid-concentrations-in-units-of-canine-packed-red-blood-cells
#9
S M Muro, J H Lee, J V Stokes, M K Ross, T M Archer, R W Wills, A J Mackin, J M Thomason
BACKGROUND: Storage of canine packed red blood cells (pRBCs) can increase erythrocyte phosphatidylserine (PS) expression and eicosanoid concentrations. HYPOTHESIS/OBJECTIVES: To determine the effects of leukoreduction on erythrocyte PS expression and eicosanoid concentrations in stored units of canine pRBCs. Our hypothesis was that leukoreduction would decrease PS expression and eicosanoid concentrations. ANIMALS: Eight healthy dogs. METHODS: In a cross-over study, units of whole blood were leukoreduced (LR) or non-LR and stored (10 and 21 days) as pRBCs...
January 31, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/28133930/in-vitro-evaluation-of-selective-inhibitors-of-nuclear-export-sine-drugs-kpt-185-and-kpt-335-against-canine-mammary-carcinoma-and-transitional-cell-carcinoma-tumor-initiating-cells
#10
J E Grayton, T Miller, H Wilson-Robles
No abstract text is available yet for this article.
January 30, 2017: Veterinary and Comparative Oncology
https://www.readbyqxmd.com/read/28133824/a-retrospective-study-comparing-histopathological-and-immunopathological-features-of-nasal-planum-dermatitis-in-20-dogs-with-discoid-lupus-erythematosus-or-leishmaniosis
#11
Michela De Lucia, Giorgia Mezzalira, Mar Bardagí, Dolors M Fondevila, Elisabetta Fabbri, Alessandra Fondati
BACKGROUND: In areas endemic for leishmaniosis, discoid lupus erythematosus (DLE) and canine leishmaniosis (CanL) are the most common differential diagnoses for nasal planum erosive-ulcerative dermatitis in dogs. HYPOTHESIS/OBJECTIVE: To compare histopathological and immunopathological features of canine nasal planum erosive-ulcerative dermatitis with depigmentation due to DLE or CanL. ANIMALS: Nasal planum biopsies from dogs with nasal planum loss of architecture, depigmentation, swelling, erosions or ulcerations due to DLE (n = 14) or CanL (n = 6)...
January 29, 2017: Veterinary Dermatology
https://www.readbyqxmd.com/read/28129095/t-lymphocytes-in-histiocytic-sarcomas-of-flat-coated-retriever-dogs
#12
A Marcinowska, F Constantino-Casas, T Williams, T Hoather, B Blacklaws, J Dobson
Flat-Coated Retriever dogs are predisposed to the development of histiocytic sarcoma (HS), a poorly differentiated, highly malignant neoplasm. The authors have previously documented a significant lymphocytic infiltrate in such tumors. The objective of this study was to examine the presence and expression of regulatory T cells in HS tumor samples. Forty tumors were included in this study. All tumors were immunolabeled for CD3, CD79a, CD25, CD45RA, and FOXP3. The proportion of positive cells was compared between tumors presenting as a localized primary soft tissue mass (soft tissue origin HS) and disseminated HS affecting viscera, especially the spleen (splenic origin HS)...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28120417/pd-1-expression-by-canine-t-cells-and-functional-effects-of-pd-1-blockade
#13
J Coy, A Caldwell, L Chow, A Guth, S Dow
The co-inhibitory checkpoint molecule programmed death receptor 1 (PD-1) can trigger T cell functional exhaustion upon binding to its ligand PD-L1 expressed on tumour cells or macrophages. PD-1 blocking antibodies have generated remarkable results in human cancer patients, including inducing durable responses in a number of advanced cancers. Therefore, monoclonal antibodies specific for canine PD-1 were assessed for T cell binding and induction of functional activation. A total of 5-10% of CD4 T cells and 20-25% of CD8 T cells from healthy dogs expressed PD-1, and PD-1 expression was upregulated on T cells from dogs with cancer...
January 25, 2017: Veterinary and Comparative Oncology
https://www.readbyqxmd.com/read/28114998/th17-skewed-immune-response-and-cluster-of-differentiation-40-ligand-expression-in-canine-steroid-responsive-meningitis-arteritis-a-large-animal-model-for-neutrophilic-meningitis
#14
Jessica Freundt-Revilla, Arianna Maiolini, Regina Carlson, Martin Beyerbach, Kai Rentmeister, Thomas Flegel, Andrea Fischer, Andrea Tipold
BACKGROUND: Steroid-responsive meningitis-arteritis (SRMA) is an immune-mediated disorder characterized by neutrophilic pleocytosis and an arteritis particularly in the cervical leptomeninges. Previous studies of the disease have shown increased levels of IL-6 and TGF-ß1 in cerebrospinal fluid (CSF). In the presence of these cytokines, naive CD4+ cells differentiate into Th17 lymphocytes which synthesize interleukin 17 (IL-17). It has been shown that IL-17 plays an active role in autoimmune diseases, it induces and mediates inflammatory responses and has an important role in recruitment of neutrophils...
January 23, 2017: Journal of Neuroinflammation
https://www.readbyqxmd.com/read/28113036/clinical-and-histopathologic-characterization-of-canine-chronic-ulcerative-stomatitis
#15
J G Anderson, S Peralta, A Kol, P H Kass, B Murphy
Canine chronic ulcerative stomatitis, also known as chronic ulcerative paradental stomatitis, is a painful condition of the oral cavity. The purpose of this study was to determine if there are commonalities in clinical and radiographic features among patients, whether the histopathologic evaluation might inform the pathogenesis, and whether the condition appears similar to human oral mucosal diseases. To do this, we prospectively collected clinical, radiographic, and histopathologic data from 20 dogs diagnosed with the disease...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28111882/programmed-death-ligand-1-is-expressed-in-canine-b-cell-lymphoma-and-downregulated-by-mek-inhibitors
#16
S R Kumar, D Y Kim, C J Henry, J N Bryan, K L Robinson, A M Eaton
Programmed death ligand 1 (PD-L1) expression in antigen-presenting cells and tumors can inhibit T cell-mediated immunity. In this study, PD-L1 mRNA and protein expression was evaluated in canine B cell lymphoma (CLL17-71), large T-cell leukemia (CLGL-90), B cell leukemia (GL-1) and primitive leukocyte round cell neoplasia (CLL-1390). Variable PD-L1 mRNA and protein were observed in these cells with high endogenous expression present in CLL17-71 cells. PD-L1 protein was also observed in canine patient B cell lymphoma tissues using immunostaining...
January 22, 2017: Veterinary and Comparative Oncology
https://www.readbyqxmd.com/read/28089546/expression-of-interleukin-6-by-a-recombinant-rabies-virus-enhances-its-immunogenicity-as-a-potential-vaccine
#17
Jun Luo, Boyue Zhang, Yuting Wu, Qin Tian, Jing Zhao, Ziyu Lyu, Qiong Zhang, Mingzhu Mei, Yongwen Luo, Xiaofeng Guo
Several studies have confirmed that interleukin-6 (IL6) mediates multiple biological effects that enhance immune responses when used as an adjuvant. In the present study, recombinant rabies virus (RABV) expressing canine IL6 (rHEP-CaIL6) was rescued and its pathogenicity and immunogenicity were investigated in mice. We demonstrated that mice received a single intramuscular immunization with rHEP-CaIL6 showed an earlier increase and higher maximum titres of virus-neutralizing antibody (VNA) as well as anti-RABV antibodies compared with mice immunized with the parent strain...
January 12, 2017: Vaccine
https://www.readbyqxmd.com/read/28089357/localization-of-toll-like-receptor-tlr-2-and-tlr4-mrna-in-the-colorectal-mucosa-of-miniature-dachshunds-with-inflammatory-colorectal-polyps
#18
N Yokoyama, H Ohta, J Yamazaki, Y Kagawa, O Ichii, N Khoirun, T Morita, T Osuga, S Y Lim, N Sasaki, K Morishita, K Nakamura, M Takiguchi
Inflammatory colorectal polyps (ICRPs) are characterized by the formation of multiple or solitary polyps with marked neutrophil infiltration in the colorectal area, and are speculated to be a novel form of breed-specific canine idiopathic inflammatory bowel disease (IBD). In human IBD, toll-like receptor (TLR) 2 and TLR4 have been reported to be involved in the pathogenesis of the disease. The aim of this study was to evaluate the expression of TLR2 and TLR4 mRNA in the colorectal mucosa of dogs with ICRPs by in-situ hybridization using an RNAscope assay...
January 12, 2017: Journal of Comparative Pathology
https://www.readbyqxmd.com/read/28060678/canine-central-nervous-system-neoplasm-phenotyping-using-tissue-microarray-technique
#19
I Spitzbarth, F Heinrich, V Herder, T Recker, P Wohlsein, W Baumgärtner
Tissue microarrays (TMAs) represent a useful technique for the simultaneous phenotyping of large sample numbers and are particularly suitable for histopathologic tumor research. In this study, TMAs were used to evaluate semiquantitatively the expression of multiple antigens in various canine central nervous system (CNS) neoplasms and to identify markers with potential discriminative diagnostic relevance. Ninety-seven canine CNS neoplasms, previously diagnosed on hematoxylin and eosin sections according to the World Health Organization classification, were investigated on TMAs, with each tumor consisting of 2 cylindrical samples from the center and the periphery of the neoplasm...
January 1, 2017: Veterinary Pathology
https://www.readbyqxmd.com/read/28056234/-effect-of-intracoronary-autologous-bone-marrow-mononuclear-cells-transplantation-on-arrhythmia-in-canines
#20
J Tang, G Y Sun, T Chen, Y D Wang, J Zhang, X Q Qi
Objective: To observe the survival and the differentiation of grafted bone marrow cells (BM-MNCs) in host myocardium. To observe whether BM-MNCs transplantation can potentially cause arrhythmia and whether the BM-MNCs transplantation can alter the spatial distribution of connexins, important mediator for arrhythmia genesis after myocardial infarction. Methods: Acute myocardial infarction (AMI) was induced by left anterior descending coronary artery (LAD) ligation in hybrid canine. BM-MNCs suspension was prepared by density centrifugation...
December 24, 2016: Zhonghua Xin Xue Guan Bing za Zhi
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