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Canine T cells

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https://www.readbyqxmd.com/read/29141366/-in-vitro-early-detection-of-amyloid-plaques-in-alzheimer-s-disease-by-pittsburgh-compound-b-modified-magnetic-nanoparticles
#1
J Q Zeng, J Q Wu, M H Li, P J Wang
Objective: To construct magnetic nanoparticles targeting β-amyloid (Aβ) plaques, the pathological biomarker of Alzheimer's disease (AD) and to study their binding capability in vitro. Methods: Superparamagnetic nanoparticles Mn(0.6)Zn(0.4)Fe(2)O(4) (MZF) were coated with amphiphilic star-block copolymeric micelles and modified with Aβ-specific probe Pittsburgh compound B (PiB) to construct a novel magnetic nanoparticle MZF-PiB, which specifically targeted amyloid plaques. Transmission electron microscope was used to study the morphological features of MZF-PiB...
November 7, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/29117254/link-n-the-missing-link-towards-intervertebral-disc-repair-is-species-specific
#2
Frances C Bach, Lisanne T Laagland, Michael P Grant, Laura B Creemers, Keita Ito, Björn P Meij, Fackson Mwale, Marianna A Tryfonidou
INTRODUCTION: Degeneration of the intervertebral disc (IVD) is a frequent cause for back pain in humans and dogs. Link-N stabilizes proteoglycan aggregates in cartilaginous tissues and exerts growth factor-like effects. The human variant of Link-N facilitates IVD regeneration in several species in vitro by inducing Smad1 signaling, but it is not clear whether this is species specific. Dogs with IVD disease could possibly benefit from Link-N treatment, but Link-N has not been tested on canine IVD cells...
2017: PloS One
https://www.readbyqxmd.com/read/29113597/antigenicity-immunogenicity-and-protective-efficacy-of-a-conserved-leishmania-hypothetical-protein-against-visceral-leishmaniasis
#3
Daniel S Dias, Vívian T Martins, Patrícia A F Ribeiro, Fernanda F Ramos, Daniela P Lage, Grasiele S V Tavares, Débora V C Mendonça, Miguel A Chávez-Fumagalli, Jamil S Oliveira, Eduardo S Silva, Dawidson A Gomes, Michele A Rodrigues, Mariana C Duarte, Alexsandro S Galdino, Daniel Menezes-Souza, Eduardo A F Coelho
In this study, a Leishmania hypothetical protein, LiHyS, was evaluated regarding its antigenicity, immunogenicity and protective efficacy against visceral leishmaniasis (VL). Regarding antigenicity, immunoblottings and an enzyme-linked immunosorbent assay using human and canine sera showed high sensitivity and specificity values for the recombinant protein (rLiHyS) in the diagnosis of VL. When evaluating the immunogenicity of LiHyS, which is possibly located in the parasite's flagellar pocket, proliferative assays using peripheral blood mononuclear cells from healthy subjects or VL patients showed a high proliferative index in both individuals, when compared to the results obtained using rA2 or unstimulated cultures...
November 8, 2017: Parasitology
https://www.readbyqxmd.com/read/29103899/perturbation-of-the-t-cell-receptor-repertoire-occurs-with-increasing-age-in-dogs
#4
Angela Holder, Samantha M Mirczuk, Robert C Fowkes, Donald B Palmer, Richard Aspinall, Brian Catchpole
Immunosenescence is the gradual deterioration in immune system function associated with ageing. This decline is partly due to involution of the thymus, which leads to a reduction in the output of naive T cells into the circulating lymphocyte pool. Expansion of existing naive and memory T cell populations, to compensate for the reduction in thymic output, can lead to reduced diversity in the T cell repertoire with increasing age, resulting in impairment of immune responses to novel antigenic challenges, such as during infection and vaccination...
October 28, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29077583/phenotypic-characterization-of-corneal-endothelial-dystrophy-in-german-shorthaired-and-wirehaired-pointers-using-in-vivo-advanced-corneal-imaging-and-histopathology
#5
Olivia R Shull, Christopher M Reilly, Lola B Davis, Christopher J Murphy, Sara M Thomasy
PURPOSE: To evaluate corneal morphology using ultrasonic pachymetry (USP), Fourier-domain optical coherence tomography (FD-OCT), and in vivo confocal microscopy (IVCM) in 2 related canine breeds-German shorthaired pointers (GSHPs) and German wirehaired pointers (GWHPs)-with and without corneal endothelial dystrophy (CED). This condition is characterized by premature endothelial cell degeneration leading to concomitant corneal edema and is similar to Fuchs endothelial corneal dystrophy...
October 25, 2017: Cornea
https://www.readbyqxmd.com/read/29076241/oncolytic-reovirus-therapy-pilot-study-in-dogs-with-spontaneously-occurring-tumours
#6
C C Hwang, M Igase, M Sakurai, T Haraguchi, K Tani, K Itamoto, T Shimokawa, M Nakaichi, Y Nemoto, S Noguchi, M Coffey, M Okuda, T Mizuno
Oncolytic virotherapy is a novel treatment involving replication-competent virus in the elimination of cancer. We have previously reported the oncolytic effects of reovirus in various canine cancer cell lines. This study aims to establish the safety profile of reovirus in dogs with spontaneously occurring tumours and to determine a recommended dosing regimen. Nineteen dogs with various tumours, mostly of advanced stages, were treated with reovirus, ranging from 1.0 × 10(8) to 5.0 × 10(9) TCID50 given as intratumour injection (IT) or intravenous infusion (IV) daily for up to 5 consecutive days in 1 or multiple treatment cycles...
October 27, 2017: Veterinary and Comparative Oncology
https://www.readbyqxmd.com/read/29075153/sv40-large-t-antigen-disrupts-embryogenesis-of-canine-and-porcine-somatic-cell-nuclear-transfer-embryo
#7
Kiyoung Eun, Seon-Ung Hwang, Yeon Woo Jeong, Sunyoung Seo, Seon Yong Lee, Woo Suk Hwang, Sang-Hwan Hyun, Hyunggee Kim
BACKGROUND: Somatic cell nuclear transfer (SCNT) is a useful biotechnological tool for transgenic animal production using genetically modified somatic cells (GMSCs). However, there are several limitations preventing successful transgenic animal generation by SCNT, such as obtaining proper somatic donor cells with a sufficiently long life span and proliferative capacity for generating GMSCs. Here, we established simian virus 40 large T antigen (SV40LT)-mediated lifespan-extended canine fibroblast cells (SV40LT-K9 cells) and evaluated their potential as nuclei donors for SCNT, based on cellular integrity and SCNT embryo development...
2017: Biological Procedures Online
https://www.readbyqxmd.com/read/29074301/identification-of-immune-biomarkers-related-to-disease-progression-and-treatment-efficacy-in-human-visceral-leishmaniasis
#8
Áquila S B Portela, Lourena E Costa, Beatriz C S Salles, Mariana P Lima, Thaís T O Santos, Fernanda F Ramos, Daniela P Lage, Vívian T Martins, Rachel B Caligiorne, Daniela R Lessa, Fabiana R Silva, Amanda S Machado, Guilherme F Nascimento, Isabela S Gama, Miguel A Chávez-Fumagalli, Antonio L Teixeira, Manoel O C Rocha, Regina L Rocha, Eduardo A F Coelho
Visceral leishmaniasis (VL) is a potentially fatal disease, in which the treatment based on chemotherapy is considered toxic. The cure of disease is associated with the life-long Th1-type immunity against the infection. The Th1-related cytokines production by peripheral blood mononuclear cells (PBMCs) seems to be crucial for host control of parasite load and clinical cure. In the current study, we used five proteins (IgE-dependent histamine-releasing factor [HRF], LiHyD, LiHyV, LiHyT and LiHyp6) recently shown to be antigenic and/or immunogenic in the canine VL, aiming to evaluate the antigen-specific antibody levels and cytokine production in PBMCs culture supernatants collected from VL patients before and after anti-VL treatment...
October 20, 2017: Immunobiology
https://www.readbyqxmd.com/read/29065140/novel-insights-into-the-pathways-regulating-the-canine-hair-cycle-and-their-deregulation-in-alopecia-x
#9
Magdalena A T Brunner, Vidhya Jagannathan, Dominik P Waluk, Petra Roosje, Monika Linek, Lucia Panakova, Tosso Leeb, Dominique J Wiener, Monika M Welle
Alopecia X is a hair cycle arrest disorder in Pomeranians. Histologically, kenogen and telogen hair follicles predominate, whereas anagen follicles are sparse. The induction of anagen relies on the activation of hair follicle stem cells and their subsequent proliferation and differentiation. Stem cell function depends on finely tuned interactions of signaling molecules and transcription factors, which are not well defined in dogs. We performed transcriptome profiling on skin biopsies to analyze altered molecular pathways in alopecia X...
2017: PloS One
https://www.readbyqxmd.com/read/29020838/safety-and-efficacy-of-aav5-vectors-expressing-human-or-canine-cngb3-in-cngb3-mutant-dogs
#10
Guo-Jie Ye, András M Komáromy, Caroline Zeiss, Roberto Calcedo, Christine D Harman, Kristin L Koehl, Gabriel A Stewart, Simone Iwabe, Vince A Chiodo, William W Hauswirth, Gustavo D Aguirre, Jeffrey D Chulay
Achromatopsia is an inherited retinal disorder of cone photoreceptors characterized by markedly reduced visual acuity, extreme light sensitivity, and absence of color discrimination. Approximately 50% of cases are caused by mutations in the cone photoreceptor-specific cyclic nucleotide gated channel beta subunit (CNGB3) gene. Studies in CNGB3-mutant dogs showed that subretinal injection of an AAV vector expressing human CNGB3, which has 76% amino acid identity with canine CNGB3, driven by a 2.1 kb human red cone opsin promoter (PR2...
October 11, 2017: Human Gene Therapy. Clinical Development
https://www.readbyqxmd.com/read/28992489/methotrexate-induces-high-level-of-apoptosis-in-canine-lymphoma-leukemia-cell-lines
#11
Aleksandra Pawlak, Justyna Kutkowska, Bożena Obmińska-Mrukowicz, Andrzej Rapak
INTRODUCTION: Methotrexate is an antimetabolite used in the treatment of cancer and non-malignant diseases including rheumatoid arthritis, psoriasis and graft vs. host disease. Combination therapy with methotrexate was successful in the treatment of canine lymphoma, mammary tumor and invasive urinary bladder cancer. Lymphoma, the most common hematopoietic cancer in dogs, and leukemia are sensitive to chemotherapy, which is why methotrexate may be an important treatment option for these diseases...
September 28, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28988041/recombinant-small-glutamine-rich-tetratricopeptide-repeat-containing-protein-of-leishmania-infantum-potential-vaccine-and-diagnostic-application-against-visceral-leishmaniasis
#12
Daniel S Dias, Patrícia A F Ribeiro, Vívian T Martins, Daniela P Lage, Áquila S B Portela, Lourena E Costa, Beatriz C S Salles, Mariana P Lima, Fernanda F Ramos, Thaís T O Santos, Rachel B Caligiorne, Miguel A Chávez-Fumagalli, Julia A G Silveira, Danielle F Magalhães-Soares, Denise U Gonçalves, Jamil S Oliveira, Bruno M Roatt, Mariana C Duarte, Daniel Menezes-Souza, Eduardo S Silva, Alexsandro S Galdino, Ricardo A Machado-de-Ávila, Antonio L Teixeira, Eduardo A F Coelho
Different Leishmania proteins have been evaluated in order to find a potential vaccine candidate or diagnostic marker capable of providing long lasting protection against infection or helping to identify infected mammalian hosts, respectively. However, just few molecules have fulfilled all the requirements to be evaluated. In the current study, we evaluated the prophylactic and diagnostic value against visceral leishmaniasis (VL) of a small glutamine-rich tetratricopeptide repeat-containing (SGT) protein from Leishmania infantum species...
November 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28969786/use-of-an-interferon-gamma-release-assay-igra-to-test-t-cell-responsiveness-to-soluble-leishmania-infantum-antigen-in-whole-blood-of-dogs-from-endemic-areas
#13
Lilia Zribi, Amel F El-Goulli, Meriem Ben-Abid, Mohamed Gharbi, Ines Ben-Sghaier, Imed Boufaden, Karim Aoun, Aïda Bouratbine
Interferon-Gamma (IFN-γ) Release Assays (IGRAs) are easy tests that allow rapid screening of primed memory T-cells immunity in response to antigen. The aim of this study was to use IGRA to assess IFN-γ release in response to Soluble Leishmania infantum antigen (SLA) in whole blood of dogs living in endemic area of visceral leishmaniasis and to interpret IGRA results according to clinical examination, specific anti-Leishmania humoral response and presence of L. infantum DNA in blood. The study was carried out on 56 dogs living in greater Tunis area...
November 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28959348/in-silico-perspectives-on-the-prediction-of-the-plp-s-epitopes-involved-in-multiple-sclerosis
#14
Zahra Zamanzadeh, Mitra Ataei, Seyed Massood Nabavi, Ghasem Ahangari, Mehdi Sadeghi, Mohammad Hosein Sanati
BACKGROUND: Multiple sclerosis (MS) is the most common autoimmune disease of the central nervous system (CNS). The main cause of the MS is yet to be revealed, but the most probable theory is based on the molecular mimicry that concludes some infections in the activation of T cells against brain auto-antigens that initiate the disease cascade. OBJECTIVES: The Purpose of this research is the prediction of the auto-antigen potency of the myelin proteolipid protein (PLP) in multiple sclerosis...
March 2017: Iranian journal of biotechnology
https://www.readbyqxmd.com/read/28959165/effects-of-electrochemotherapy-with-cisplatin-and-peritumoral-il-12-gene-electrotransfer-on-canine-mast-cell-tumors-a-histopathologic-and-immunohistochemical-study
#15
Claudia Salvadori, Tanja Svara, Guido Rocchigiani, Francesca Millanta, Darja Pavlin, Maja Cemazar, Ursa Lampreht Tratar, Gregor Sersa, Natasa Tozon, Alessandro Poli
BACKGROUND: The study was aimed to characterize tumor response after combined treatment employing electrochemotherapy with IL-12 gene electrotransfer in dogs with spontaneous mast cell tumors (MCT). MATERIALS AND METHODS: Eleven dogs with eleven MCTs were included in the study. Histological changes were investigated in biopsy specimens collected before the treatment (T0), and 4 (T1) and 8 weeks (T2) later. Cellular infiltrates were characterized immunohistochemically by using anti CD3, CD20, Foxp3 (Treg), CD68 and anti MHC-class II antibodies...
September 2017: Radiology and Oncology
https://www.readbyqxmd.com/read/28958896/anti-inducible-costimulator-monoclonal-antibody-treatment-of-canine-chronic-graft-versus-host-disease
#16
Scott S Graves, Maura H Parker, Diane Stone, George E Sale, Smitha P S Pillai, Melissa M Johnson, Rainer Storb
In murine model systems inducible costimulator (ICOS) signaling has been implicated in the formation of chronic graft-versus-host disease (GVHD). Previously, we showed that chronic GVHD can be reproducibly produced in the dog hematopoietic cell transplantation (HCT) model and that ICOS expression is upregulated on T cells in dogs with chronic GVHD. The goal of the present study was to determine whether administration of a short course of anti-canine ICOS mAb could alter the rapid and progressive course of chronic GVHD...
September 25, 2017: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/28957780/anti-tumour-efficacy-of-etoposide-alone-and-in-combination-with-piroxicam-against-canine-osteosarcoma-in-a-xenograft-model
#17
S M Ong, K Saeki, M K Kok, Y Tanaka, N Choisunirachon, R Yoshitake, R Nishimura, T Nakagawa
Osteosarcoma (OSA) in dogs is locally invasive and highly malignant. Distant metastasis is the most common cause of death. To date, the survival rate in dogs with OSA remains poor. The cytotoxic effects of etoposide against canine OSA cell lines, either alone or in combination with piroxicam, have been previously demonstrated in vitro. The aim of this study was to evaluate the anti-tumour effect of etoposide alone and in combination with piroxicam on canine OSA using murine models. Etoposide single agent treatment significantly delayed tumour progression with a marked reduction in Ki-67 immunoreactivity in tumour tissue...
September 19, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28948627/prevalence-of-exon-11-internal-tandem-duplications-in-the-c-kit-proto-oncogene-in-australian-canine-mast-cell-tumours
#18
V S Tamlin, A E Kessell, R J Mccoy, E C Dobson, T S Smith, M Hebart, L Brown, D Mitrovic, A E Peaston
OBJECTIVE: To measure the prevalence of internal tandem duplications (ITDs) in exon 11 of the proto-oncogene C-KIT in a sample of Australian cutaneous canine mast cell tumours (MCTs) drawn from general practice and to evaluate relationships between tumour mutation status and prognostic factors including signalment, tumour histological grade, tumour anatomical location and tumour size. METHODS: C-KIT exon 11 ITDs were detected by PCR in DNA extracted from formalin-fixed, paraffin-embedded canine MCTs sourced from three veterinary diagnostic laboratories in Adelaide and Melbourne...
October 2017: Australian Veterinary Journal
https://www.readbyqxmd.com/read/28924718/comprehensive-annotation-and-evolutionary-insights-into-the-canine-canis-lupus-familiaris-antigen-receptor-loci
#19
Jolyon Martin, Hannes Ponstingl, Marie-Paule Lefranc, Joy Archer, David Sargan, Allan Bradley
Dogs are an excellent model for human disease. For example, the treatment of canine lymphoma has been predictive of the human response to that treatment. However, an incomplete picture of canine (Canis lupus familiaris) immunoglobulin (IG) and T cell receptor (TR)-or antigen receptor (AR)-gene loci has restricted their utility. This work advances the annotation of the canine AR loci and looks into breed-specific features of the loci. Bioinformatic analysis of unbiased RNA sequence data was used to complete the annotation of the canine AR genes...
September 19, 2017: Immunogenetics
https://www.readbyqxmd.com/read/28906588/surface-glycan-pattern-of-canine-equine-and-ovine-bone-marrow-derived-mesenchymal-stem-cells
#20
Salvatore Desantis, Gianluca Accogli, Antonio Crovace, Edda G Francioso, Alberto Maria Crovace
The use of bone marrow-derived mesenchymal stem cells (MSCs) for clinical and experimental studies is increasing, but full characterization of MSCs in veterinary species is hindered by the variability in species-specific cell surface marker expression and antibody cross reactivity. Recent studies demonstrated that the glycans in the glycocalyx of MSCs are promising candidates as cell biomarkers. In the present study, we analyzed the glycocalyx of canine MSCs (cMSCs), ovine MSCs (oMSCs), and equine MSCs (eMSCs) using a cell microarray procedure in which MSCs were spotted on microarray slides and incubated with a panel of 14 biotinylated lectins and Cy3-conjugated streptavidin...
September 14, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
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