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Canine T cells

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https://www.readbyqxmd.com/read/27900781/the-present-and-future-of-opioid-analgesics-in-small-animal-practice
#1
REVIEW
B T Simon, P V Steagall
Opioids are the cornerstone for the treatment of acute pain in small animal patients. This is primarily because of their remarkable safety profile, high efficacy, and benefit of reversibility. There have been some significant advances in our knowledge on opioid pharmacology and clinical usage in companion animal medicine. This review discusses the progression of opioid use in small animal practice providing current misconceptions and controversies in light of routes of administration. Potential targets for research and drug development and novel therapies are discussed in addition to the concepts of glial cell modulators, individual variability, and opioid tolerance and hyperalgesia...
November 30, 2016: Journal of Veterinary Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/27893789/characterization-of-the-canine-mhc-class-i-dla-88-50101-peptide-binding-motif-as-a-prerequisite-for-canine-t-cell-immunotherapy
#2
Sharon M Barth, Christian M Schreitmüller, Franziska Proehl, Kathrin Oehl, Leonie M Lumpp, Daniel J Kowalewski, Moreno Di Marco, Theo Sturm, Linus Backert, Heiko Schuster, Stefan Stevanović, Hans-Georg Rammensee, Oliver Planz
There are limitations in pre-clinical settings using mice as a basis for clinical development in humans. In cancer, similarities exist between humans and dogs; thus, the dog patient can be a link in the transition from laboratory research on mouse models to clinical trials in humans. Knowledge of the peptides presented on MHC molecules is fundamental for the development of highly specific T cell-based immunotherapies. This information is available for human MHC molecules but is absent for the canine MHC. In the present study, we characterized the binding motif of dog leukocyte antigen (DLA) class I allele DLA-88*50101, using human C1R and K562 transfected cells expressing the DLA-88*50101 heavy chain...
2016: PloS One
https://www.readbyqxmd.com/read/27891564/a-homozygous-pign-missense-mutation-in-soft-coated-wheaten-terriers-with-a-canine-paroxysmal-dyskinesia
#3
Ana L Kolicheski, Gary S Johnson, Tendai Mhlanga-Mutangadura, Jeremy F Taylor, Robert D Schnabel, Taroh Kinoshita, Yoshiko Murakami, Dennis P O'Brien
Hereditary paroxysmal dyskinesias (PxD) are a heterogeneous group of movement disorders classified by frequency, duration, and triggers of the episodes. A young-adult onset canine PxD has segregated as an autosomal recessive trait in Soft-Coated Wheaten Terriers. The medical records and videos of episodes from 25 affected dogs were reviewed. The episodes of hyperkinesia and dystonia lasted from several minutes to several hours and could occur as often as >10/day. They were not associated with strenuous exercise or fasting but were sometimes triggered by excitement...
November 28, 2016: Neurogenetics
https://www.readbyqxmd.com/read/27890077/t-lymphocyte-immunophenotypes-in-the-cerebrospinal-fluid-of-dogs-with-visceral-leishmaniasis
#4
Fernanda G Grano, José Eduardo Dos S Silva, Guilherme D Melo, Juliana Perosso, Valéria M F Lima, Gisele F Machado
Visceral leishmaniasis (VL) is a disease causing several clinical manifestations in dogs, including neurological disorders. Nevertheless, there are few studies related to the evaluation of the brain alterations during VL. Evidences of the involvement of cerebral barriers in infected dogs was reported, including the presence of brain inflammatory infiltrate, with a predominance of CD3+ T cells. Therefore, the aim of this study was to determine the immunophenotypes of T lymphocytes in the cerebrospinal fluid (CSF), as well as in peripheral blood, and to correlate with brain alterations in dogs with VL...
December 15, 2016: Veterinary Parasitology
https://www.readbyqxmd.com/read/27889201/expression-of-fibroblast-activating-protein-and-correlation-with-histological-grade-mitotic-index-and-ki67-expression-in-canine-mast-cell-tumours
#5
A Giuliano, R Dos Santos Horta, F Costantino-Casas, T Hoather, J Dobson
Fibroblast activating protein (FAP) is a membrane serine protease expressed by activated fibroblasts, particularly tumour associated fibroblasts (TAFs). FAP expression has not been reported in canine mast cell tumours (MCTs). The objective of this study was to evaluate the expression of FAP in TAFs and its correlation with histological grade, mitotic index and Ki67 expression in canine MCTs. FAP expression was evaluated by immunohistochemistry (IHC) in 30 canine MCTs. Twenty-eight (90%) of the MCTs expressed FAP in the stroma, 16 cases showed low to intermediate FAP score and 14 cases had a high FAP score...
November 24, 2016: Journal of Comparative Pathology
https://www.readbyqxmd.com/read/27881051/mechanisms-of-immune-suppression-utilized-by-canine-adipose-and-bone-marrow-derived-mesenchymal-stem-cells
#6
Lyndah Chow, Valerie Johnson, Jonathan W Coy, Daniel P Regan, Steven W Dow
Mesenchymal stem cells (MSC) from rodents and humans have been shown to suppress T cells by distinct primary pathways, with NO-dependent pathways dominating in rodents and IDO-dependent pathways dominating in humans. However, the immune suppressive pathways utilized by canine MSC have not been as thoroughly studied, nor have BM-MSC and Ad-MSC been directly compared for their immune modulatory potency or pathway utilization. Therefore, canine BM-MSC and Ad-MSC were generated in vitro and their potency in suppressing T cell proliferation and cytokine production was compared, as well as differential gene expression...
November 23, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27863558/development-of-an-autologous-canine-cancer-vaccine-system-for-resectable-malignant-tumors-in-dogs
#7
J R Yannelli, R Wouda, T J Masterson, M G Avdiushko, D A Cohen
While conventional therapies exist for canine cancer, immunotherapies need to be further explored and applied to the canine setting. We have developed an autologous cancer vaccine (K9-ACV), which is available for all dogs with resectable disease. K9-ACV was evaluated for safety and immunogenicity for a variety of cancer types in a cohort of companion dogs under veterinary care. The autologous vaccine was prepared by enzymatic digestion of solid tumor biopsies. The resultant single cell suspensions were then UV-irradiated resulting in immunogenic cell death of the tumor cells...
December 2016: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/27863541/cellular-endocytic-compartment-localization-of-expressed-canine-cd1-molecules
#8
Mette Schjaerff, Stefan M Keller, Verena K Affolter, Annemarie T Kristensen, Peter F Moore
CD1 molecules are glycoproteins present primarily on dendritic cells (DCs), which recognize and present a variety of foreign- and self-lipid antigens to T-cells. Humans have five different CD1 isoforms that survey distinct cellular compartments allowing for recognition of a large repertoire of lipids. The canine CD1 family consists of seven functional CD1 molecules (canine CD1a2, CD1a6, CD1a8, CD1a9, CD1b, CD1c and CD1e) and one presumed non-functional isoform (canine CD1d) due to a disrupted gene structure...
December 2016: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/27863540/comparison-of-serum-cytokine-levels-between-dogs-with-multicentric-lymphoma-and-healthy-dogs
#9
Jerome Calvalido, Geoffrey A Wood, Anthony J Mutsaers, Darren Wood, William Sears, J Paul Woods
In humans, multiple cytokines have been linked to the development of lymphoma, and are relevant biomarkers for response to chemotherapy and prognosis. In contrast, only a few circulating cytokines have been studied in dogs with lymphoma. We prospectively enrolled thirty-one dogs newly diagnosed with multicentric lymphoma. Immunophenotype was determined by flow cytometry in all dogs, separating them into 2 subgroups: B cell lymphoma (n=21) and T cell lymphoma (n=10). Nineteen healthy dogs were enrolled in the control group...
December 2016: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/27855679/a-pilot-study-of-toceranib-vinblastine-therapy-for-canine-transitional-cell-carcinoma
#10
Sarah B Rippy, Heather L Gardner, Sandra M Nguyen, Emma E Warry, Roberta A Portela, William Tod Drost, Eric T Hostnik, Eric M Green, Dennis J Chew, Juan Peng, Cheryl A London
BACKGROUND: Effective therapies for transitional cell carcinoma (TCC) are limited, with objective response rates to most chemotherapeutic regimens below 20%. The purpose of this study was to investigate the biologic activity of combined toceranib phosphate and vinblastine chemotherapy for treatment of TCC. A secondary objective was to compare the utility of Computed Tomography (CT) and abdominal ultrasound (AUS) in tumor response assessments. RESULTS: Dogs with TCC received vinblastine at 1...
November 17, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27852854/a-novel-rabies-vaccine-expressing-cxcl13-enhances-humoral-immunity-by-recruiting-both-t-follicular-helper-and-germinal-center-b-cells
#11
Zhao Wang, Mingming Li, Ming Zhou, Yajing Zhang, Jie Yang, Yandi Cao, Kunlun Wang, Min Cui, Huanchun Chen, Zhen F Fu, Ling Zhao
: Rabies still remains a public health threat in most parts of the world, and approximately 99% of the cases are transmitted by dogs. There is an urgent need to develop an efficacious and affordable vaccine to control canine-transmitted rabies in developing countries. Our previous studies demonstrate that overexpression of chemokines/cytokines such as CCL-3 (MIP-1α) and GM-CSF can enhance the immunogenicity of rabies vaccines. In the present study, the chemokine CXCL13 was inserted into the genome of the recombinant rabies virus (rRABV) strain LBNSE, and the effect of the chemokine CXCL13 on the immunogenicity of the RABV was investigated...
November 16, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27842458/suppression-of-canine-dendritic-cell-activation-maturation-and-inflammatory-cytokine-release-by-mesenchymal-stem-cells-occurs-via-multiple-distinct-biochemical-pathways
#12
William Henry Wheat, Lyndah Chow, Jade N Kurihara, Daniel P Regan, Jonathan W Coy, Tracy L Webb, Steven W Dow
Mesenchymal stem cells (MSC) represent a readily accessible source of cells with potent immune modulatory activity. MSC can suppress ongoing inflammatory responses by suppressing T cell function, while fewer studies have examined the impact of MSC on dendritic cell function. The dog spontaneous disease model represents an important animal model with which to evaluate the safety and effectiveness of cellular therapy with MSC. This study evaluated the effects of canine MSC on the activation and maturation of canine monocyte-derived dendritic cells (DC), as well as mechanisms underlying these effects...
November 14, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27829290/hsp90-inhibitors-in-the-context-of-heat-shock-and-the-unfolded-protein-response-effects-on-a-primary-canine-pulmonary-adenocarcinoma-cell-line
#13
Arin N Graner, Justin E Hellwinkel, Alex M Lencioni, Helen J Madsen, Tessa A Harland, Paul Marchando, Ger J Nguyen, Mary Wang, Laura M Russell, Lynne T Bemis, Thomas J Anchordoquy, Michael W Graner
BACKGROUND: Agents targeting HSP90 and GRP94 are seldom tested in stressed contexts such as heat shock (HS) or the unfolded protein response (UPR). Tumor stress often activates HSPs and the UPR as pro-survival mechanisms. This begs the question of stress effects on chemotherapeutic efficacy, particularly with drugs targeting chaperones such as HSP90 or GRP94. We tested the utility of several HSP90 inhibitors, including PU-H71 (targeting GRP94), on a primary canine lung cancer line under HS/UPR stress compared to control conditions...
November 9, 2016: International Journal of Hyperthermia
https://www.readbyqxmd.com/read/27824893/an-age-associated-decline-in-thymic-output-differs-in-dog-breeds-according-to-their-longevity
#14
Angela Holder, Stephanie Mella, Donald B Palmer, Richard Aspinall, Brian Catchpole
The age associated decline in immune function is preceded in mammals by a reduction in thymic output. Furthermore, there is increasing evidence of a link between immune competence and lifespan. One approach to determining thymic output is to quantify signal joint T cell receptor excision circles (sj-TRECs), a method which has been developed and used in several mammalian species. Life expectancy and the rate of aging vary in dogs depending upon their breed. In this study, we quantified sj-TRECs in blood samples from dogs of selected breeds to determine whether there was a relationship between longevity and thymic output...
2016: PloS One
https://www.readbyqxmd.com/read/27774699/characterization-of-teratogenic-potential-and-gene-expression-in-canine-and-feline-amniotic-membrane-derived-stem-cells
#15
M T Cardoso, A O Pinheiro, A S Vidane, J B Casals, V C de Oliveira, Njn Gonçalves, D S Martins, C E Ambrósio
The biosafety of innovative procedures that utilize stem cells in regenerative medicine has been addressed in several studies. Previous work has showed no tumour formation following the use of feline and human amniotic membrane-derived stem cells (AMSCs). In contrast, tumour formation was observed when canine AMSCs were utilized. These findings suggested that feline and human, but not canine, AMSCs are suitable for cell transplantation trials. This study aimed to further evaluate the feasibility of utilizing canine AMSCs for transplantation purposes as well as for felines...
October 23, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27774662/kisspeptin-10-and-the-g-protein-coupled-receptor-54-are-differentially-expressed-in-the-canine-pregnant-uterus-and-trophoblast-cells
#16
S Schäfer-Somi, S S Ay, D Kaya, M Sözmen, H B Beceriklisoy, A R Ağaoğlu, M Fındık, T van Haeften, S Aslan
Uterine tissue was collected from bitches after ovariohysterectomy at different times after ovulation. Samples were assigned to four groups: metestrous non-pregnant, day 10-12, n = 4; pre-implantation, day 10-12, n = 9; post-implantation, day 18-25, n = 13; mid-gestation, day 30-40, n = 7. RT-qPCR detection was performed for kiss1 and the G protein-coupled receptor 54 (GPR54, specific receptor for kisspeptin). In addition, immunohistochemistry was performed for detection of kisspeptin-10 (KP-10), GPR54, as well as pan-cytokeratin and vimentin...
October 23, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27774658/the-localization-of-kisspeptin-and-kisspeptin-receptor-in-the-canine-ovary-during-different-stages-of-the-reproductive-cycle
#17
M E Cielesh, B M McGrath, C J Scott, S T Norman, C P Stephen
Kisspeptin is a neuropeptide involved in the hypothalamic regulation of reproduction in many species. Recent studies have revealed kisspeptin within the ovaries of rats, Siberian hamsters and humans, indicating a local role in reproduction. However, the role of kisspeptin in the ovary is poorly understood in the bitch. This study investigated the presence and location of kisspeptin protein (KISS1) and kisspeptin receptors (KISS1R) in the canine ovary during different stages of the reproductive cycle (pre-pubertal, anoestrus and cycling) by means of immunohistochemical staining...
October 23, 2016: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/27742446/recombinant-canine-adenovirus-type-2-expressing-tgrop16-provides-partial-protection-against-acute-toxoplasma-gondii-infection-in-mice
#18
Xiu-Zhen Li, Lin Lv, Xu Zhang, Kenneth Yongabi Anchang, Auwalu Yusuf Abdullahi, Liqing Tu, Xiaohu Wang, Lijun Xia, Xiu-Xiang Zhang, Weili Feng, Chunxia Lu, Shoujun Li, Zi-Guo Yuan
We previously demonstrated that the survival time of BALB/c mice challenged with Toxoplasma gondii RH strain was prolonged by immunising the mice with a eukaryotic vector expressing the protein ROP16 of T. gondii. Building upon previous findings, we are exploring improved vaccination strategies to enhance protection. In this work, a novel recombinant canine adenovirus type 2 expressing ROP16 (CAV-2-ROP16) of T. gondii was constructed and identified to express ROP16 in Madin-Darby canine kidney cells (MDCK) cells by western blot (WB) and indirect immunofluorescence (IFA) assays...
October 11, 2016: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/27736777/genome-wide-association-study-of-cell-mediated-response-in-dogs-naturally-infected-by-leishmania-infantum
#19
Luís F S Batista, Yuri T Utsunomiya, Thaís B F Silva, Raíssa A Dias, Thaise Y Tomokane, Acácio D Pacheco, Vânia L R da Matta, Fernando T Silveira, Mary Marcondes, Cáris M Nunes, Márcia D Laurenti
A genome-wide association study (GWAS) could unravel the complexity of the cell-mediated immunity (CMI) to canine leishmaniasis (CanL). Therefore, we scanned 110,165 single-nucleotide polymorphisms (SNPs), aiming to identify chromosomal regions associated with the leishmanin skin test (LST), lymphocyte proliferation assay (LPA), and cytokine responses to further understand the role played by CMI in the outcome of natural Leishmania infantum infection in 189 dogs. Based on LST and LPA, four CMI profiles were identified (LST(-)/LPA(-), LST(+)/LPA(-), LST(-)/LPA(+), and LST(+)/LPA(+)), which were not associated with subclinically infected or diseased dogs...
December 2016: Infection and Immunity
https://www.readbyqxmd.com/read/27689742/toxicity-and-efficacy-of-cdo-nanostructures-on-the-mdck-and-caki-2-cells
#20
T V M Sreekanth, Muthuraman Pandurangan, G R Dillip, Doo Hwan Kim, Yong Rok Lee
This article reports the toxicological effects of synthesized cadmium oxide (CdO) nanostructures via a simple green route using a Polygala tenuifolia root extract on normal and renal tumor cells. First, the formation of cadmium oxide nanostructures were confirmed structurally by Fourier transform infrared spectroscopy, X-ray diffraction, and X-ray photoelectron spectroscopy (XPS). The powder was crystallized in a cubic structure with a space group of Fm-3m. The mean crystallize size was approximately 40 and 44nm from the Scherrer and size-strain plots, respectively...
September 23, 2016: Journal of Photochemistry and Photobiology. B, Biology
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