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https://www.readbyqxmd.com/read/29223880/tomato-plants-use-non-enzymatic-antioxidant-pathways-to-cope-with-moderate-uv-a-b-irradiation-a-contribution-to-the-use-of-uv-a-b-in-horticulture
#1
N Mariz-Ponte, R J Mendes, S Sario, J M P Ferreira de Oliveira, P Melo, C Santos
Plants developed receptors for solar UV-A/B radiation, which regulate a complex network of functions through the plant's life cycle. However, greenhouse grown crops, like tomato, are exposed to strongly reduced UV radiation, contrarily to their open-field counterparts. A new paradigm of modern horticulture is to supplement adequate levels of UV to greenhouse cultures, inducing a positive mild stress necessary to stimulate oxidative stress pathways and antioxidant mechanisms. Protected cultures of Solanum (cv MicroTom) were supplemented with moderate UV-A (1h and 4h) and UV-B (1min and 5min) doses during the flowering/fruiting period...
December 6, 2017: Journal of Plant Physiology
https://www.readbyqxmd.com/read/29219208/lncrna-h19-promotes-epithelial-mesenchymal-transition-emt-by-targeting-mir-484-in-human-lung-cancer-cells
#2
Qianqian Zhang, Xiaoliang Li, Xiao Li, Xiaosu Li, Zhuochang Chen
BACKGROUND: Recently, the long non-coding RNA (lncRNA) H19 has been identified as an oncogenic gene in multiple cancer types. However, the molecular basis for this observation has not been characterized in lung cancer, especially during epithelial mesenchymal transition (EMT) progression. METHODS: Cell viability, migration, invasion, and apoptosis were measured using trypan blue exclusion assay, Transwell migration/invasion assay, andflow cytometry, respectively...
December 8, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29214167/comparison-of-us-strain-elastography-and-entero-mri-to-typify-the-mesenteric-and-bowel-wall-changes-during-crohn-s-disease-a-pilot-study
#3
Giuseppe Lo Re, Dario Picone, Federica Vernuccio, Laura Scopelliti, Ambra Di Piazza, Chiara Tudisca, Salvatore Serraino, Giambattista Privitera, Federico Midiri, Sergio Salerno, Massimo Midiri, Tommaso Vincenzo Bartolotta, Roberto Lagalla
Purpose: To evaluate and compare the mesenteric and bowel wall changes during Crohn's disease (CD) on ultrasonography (US) Strain Elastography (SE) and Enterography Magnetic Resonance Imaging (E-MRI). Methods: From July 2014 to September 2016, 35 patients with ileocolonoscopy diagnosis of CD were prospectively examined with E-MRI and in the same time with US and SE. Results: A total of 41 affected bowel segments and 35 unaffected bowel segments in 35 patients were evaluated...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29204178/biological-evaluation-docking-and-molecular-dynamic-simulation-of-some-novel-diaryl-urea-derivatives-bearing-quinoxalindione-moiety
#4
Sedighe Sadeghian-Rizi, Ghadamali Ali Khodarahmi, Amirhossein Sakhteman, Ali Jahanian-Najafabadi, Mahboubeh Rostami, Mahmoud Mirzaei, Farshid Hassanzadeh
In this study a series of diarylurea derivatives containing quinoxalindione group were biologically evaluated for their cytotoxic activities using MTT assay against MCF-7 and HepG2 cell lines. Antibacterial activities of these compounds were also evaluated by Microplate Alamar Blue Assay (MABA) against three Gram-negative (Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi), three Gram-positive (Staphylococcus aureus, Bacillus subtilis and Listeria monocitogenes) and one yeast-like fungus (Candida albicans) strain...
December 2017: Research in Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29202831/heterologous-expression-purification-and-function-of-the-extracellular-domain-of-human-rank
#5
Yilei Wei, Yu Zhan, Pengfei Chen, Zhi Liu, Haohao Zhang, Dandan Liu, Jie Zhang, Min Yu, Wei Mo, Jun Zhang, Xiaoren Zhang
BACKGROUND: Receptor activator of NF-κB ligand (RANKL)/RANK signaling essentially functions within the skeletal system, particularly participating in osteoclastogenesis and bone resorption. In addition, this signaling pathway has also been shown to influence tumor progression as well as the development and function of the immune system. Therefore, blocking the interaction between RANKL and RANK is a new therapeutic approach to prevent bone-related diseases and cancer. RESULTS: The coding sequence encoding the extracellular domain of human RANK (RANK-N) was codon optimized for Pichia pastoris and cloned into the pPIC9K vector, and the recombinant plasmid was then transformed into P...
December 4, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/29190590/the-impact-of-simulation-based-education-on-nursing-confidence-knowledge-and-patient-outcomes-on-general-medicine-units
#6
Sarah Crowe, Lisa Ewart, Sarah Derman
The ability to quickly and reliably detect a clinically deteriorating patient and intervene appropriately is a skill nurses are expected to bring into practice. Simulation education has been shown to improve nurses' knowledge, confidence and communication, especially when focused on deteriorating patients. In a simulation center at a large tertiary level Canadian teaching hospital, a 4 h simulation based education session was developed for general medicine nursing staff. The education included a didactic lecture followed by four 40 min simulation scenarios focused on deteriorating patient...
November 26, 2017: Nurse Education in Practice
https://www.readbyqxmd.com/read/29183045/peripapillary-microvascular-improvement-and-lamina-cribrosa-depth-reduction-after-trabeculectomy-in-primary-open-angle-glaucoma
#7
Joong Won Shin, Kyung Rim Sung, Ki Bang Uhm, Jaehyuck Jo, Yeji Moon, Min Kyung Song, Ji Yoon Song
Purpose: To evaluate peripapillary microvascular changes in patients with primary open-angle glaucoma (POAG) after trabeculectomy using optical coherence tomography (OCT) angiography, and to determine the influence of lamina cribrosa (LC) displacement on changes in peripapillary microvasculature. Methods: The peripapillary retinal microvasculature and LC were imaged using OCT angiography and OCT-enhanced depth imaging, respectively. The microvasculature and LC depth (LCD) were measured before, and 1 week, 1 month, and 3 months after trabeculectomy...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29182359/validation-of-a-claims-based-algorithm-to-characterize-episodes-of-care
#8
Chad Ellimoottil, John D Syrjamaki, Benedict Voit, Vinay Guduguntla, David C Miller, James M Dupree
OBJECTIVES: Although hospitals face increasing pressure from payers to improve the efficiency of healthcare delivery beyond the index hospitalization, they often lack information on postdischarge events. The Michigan Value Collaborative (MVC) developed a claims-based algorithm to provide hospitals with data on events that occur to patients beyond the hospitalization. Herein, we discuss the validation of MVC's claims-based algorithm. STUDY DESIGN: Retrospective analysis of a claims-based algorithm's ability to identify specific medical events, such as index hospitalizations, 30-day readmissions, emergency department visits, skilled nursing facility admissions, home health visits, and rehabilitation services...
November 1, 2017: American Journal of Managed Care
https://www.readbyqxmd.com/read/29174564/crispr-cas9-engineered-osteogenesis-imperfecta-type-v-leads-to-severe-skeletal-deformities-and-perinatal-lethality-in-mice
#9
Frank Rauch, Yeqing Geng, Lisa Lamplugh, Bahareh Hekmatnejad, Marie-Hélène Gaumond, Janice Penney, Yojiro Yamanaka, Pierre Moffatt
Osteogenesis imperfecta (OI) type V is caused by an autosomal dominant mutation in the IFITM5 gene, also known as BRIL. The c.-14C>T mutation in the 5'UTR of BRIL creates a novel translational start site adding 5 residues (MALEP) in frame with the natural coding of BRIL. A neomorphic function has been proposed for the MALEP-BRIL but the mechanisms at play are still unknown. In order to further understand the effects of MALEP-BRIL in vivo, we generated a knockin (KI) mouse model having the exact genetic -14C>T replica of patients with OI type V...
November 22, 2017: Bone
https://www.readbyqxmd.com/read/29137095/differences-in-ugt1a1-gene-mutations-and-pathological-liver-changes-between-chinese-patients-with-gilbert-syndrome-and-crigler-najjar-syndrome-type-ii
#10
Lei Sun, Man Li, Liang Zhang, Xiaoying Teng, Xiangmei Chen, Xingang Zhou, Zhiyuan Ma, Liming Qi, Peng Wang
Diagnosis of Crigler-Najjar syndrome type II (CNS-II) and Gilbert syndrome (GS) based on the serum bilirubin concentration is difficult, because this parameter can fluctuate under certain conditions. The aim of this study was to explore differences in UGT1A1 gene mutations, which cause both CNS and GS, and pathological changes between CNS-II and GS.Ninety-five Chinese patients with hereditary unconjugated hyperbilirubinemia were enrolled in this study. Peripheral blood samples obtained from patients were used to evaluate bilirubin levels and for UGT1A1 gene testing...
November 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29105785/a-color-code-for-glycosaminoglycans-identification-by-means-of-polyacrylamide-gel-electrophoresis-stained-with-the-cationic-carbocyanine-dye-stains-all
#11
João Pedro Souza Andrade, Caroline Pacheco Oliveira, Ana Maria Freire Tovar, Paulo Antonio de Souza Mourão, Eduardo Vilanova
Cationic dyes such as toluidin blue are commonly employed to visualize Glycosaminoglycans (GAGs) on electrophoresis gels; however, the carbocyanine based dye Stains-all has been increasingly used to stain the non-sulfated hyaluronic acid and other GAGs in submicrogram quantities. In this short communication, we demonstrate that Stains-all is able to stain the most common GAGs on polyacrylamide gels with distinct and contrasting colors in a reproducible manner. We also show that this staining method is useful to identify GAGs present both in mixtures and in submicrogram quantities...
November 6, 2017: Electrophoresis
https://www.readbyqxmd.com/read/29097448/using-simulation-to-develop-care-models-for-rapid-response-and-code-teams-at-a-satellite-facility
#12
Amy R L Rule, Julie Snider, Cheryl Marshall, Kathleen Kramer, Gary L Geis, Ken Tegtmeyer, Craig H Gosdin
BACKGROUND: Our institution recently completed an expansion of an acute care inpatient unit within a satellite hospital that does not include an on-site ICU or PICU. Because of expected increases in volume and acuity, new care models for Rapid Response Teams (RRTs) and Code Blue Teams were necessary. OBJECTIVES: Using simulation-based training, our objectives were to define the optimal roles and responsibilities for team members (including ICU physicians via telemedicine), refine the staffing of RRTs and code Teams, and identify latent safety threats (LSTs) before opening the expanded inpatient unit...
November 2, 2017: Hospital Pediatrics
https://www.readbyqxmd.com/read/29047832/coded-aperture-design-in-compressive-spectral-imaging-based-on-side-information
#13
Laura Galvis, Daniel Lau, Xu Ma, Henry Arguello, Gonzalo R Arce
Coded aperture compressive spectral imagers (CSI) sense a three-dimensional data cube by using two-dimensional projections of the coded and spectrally dispersed input image. Recently, it has been shown that by combining spectral images acquired from a CSI sensor and a complementary sensor leads to substantial improvement in the quality of the fused image. To maximally exploit the benefits of the complementary information, the spatial structure of the coded apertures must be optimized inasmuch as these structures determine the sensing matrix properties and, accordingly, the quality of the reconstructed images...
August 1, 2017: Applied Optics
https://www.readbyqxmd.com/read/29046809/hospital-resuscitation-teams-a-review-of-the-risks-to-the-healthcare-worker
#14
REVIEW
Stephen M Vindigni, Juan N Lessing, David J Carlbom
BACKGROUND: "Code blue" events and related resuscitation efforts involve multidisciplinary bedside teams that implement specialized interventions aimed at patient revival. Activities include performing effective chest compressions, assessing and restoring a perfusing cardiac rhythm, stabilizing the airway, and treating the underlying cause of the arrest. While the existing critical care literature has appropriately focused on the patient, there has been a dearth of information discussing the various stresses to the healthcare team...
2017: Journal of Intensive Care
https://www.readbyqxmd.com/read/29040245/code-team-training-demonstrating-adherence-to-aha-guidelines-during-pediatric-code-blue-activations
#15
Claire Stewart, Jamie Shoemaker, Rachel Keller-Smith, Katherine Edmunds, Andrew Davis, Ken Tegtmeyer
OBJECTIVE: Pediatric code blue activations are infrequent events with a high mortality rate despite the best effort of code teams. The best method for training these code teams is debatable; however, it is clear that training is needed to assure adherence to American Heart Association (AHA) Resuscitation Guidelines and to prevent the decay that invariably occurs after Pediatric Advanced Life Support training. The objectives of this project were to train a multidisciplinary, multidepartmental code team and to measure this team's adherence to AHA guidelines during code simulation...
October 16, 2017: Pediatric Emergency Care
https://www.readbyqxmd.com/read/29028420/the-effect-of-telemedicine-on-resource-utilization-and-hospital-disposition-in-critically-ill-pediatric-transport-patients
#16
Kimberly Fugok, Nicholas B Slamon
PURPOSE: Pediatric transport teams rely on communication to report patient data to medical command officers, who create care plans and determine disposition. Common destinations are the emergency department (ED), pediatric intensive care unit (PICU), or regular inpatient care area (RIPCA). Telephone report does not result in complete understanding of the patient's condition. Further workup in the ED is often required. Telemedicine allows the patient to be directly seen; parents to be interviewed; and laboratory studies, radiographs, and vital signs to be reviewed...
October 13, 2017: Telemedicine Journal and E-health: the Official Journal of the American Telemedicine Association
https://www.readbyqxmd.com/read/29023619/the-sinus-membrane-maxillary-lateral-wall-complex-histologic-description-and-clinical-implications-for-maxillary-sinus-floor-elevation
#17
Angel Insua, Alberto Monje, Istvan Urban, Laura G Kruger, Carlos Garaicoa-Pazmiño, James V Sugai, Hom-Lay Wang
Maxillary sinus floor elevation has been documented as a safe and predictable procedure for gaining vertical bone height in the atrophic posterior maxillae. Conversely, there is a lack of basic research on the characteristics of the union between the sinus membrane (SM) and the bone. Clinical implications of an impaired union in healthy or pathologic membranes remain unknown. The objective of this study was to present a comprehensive histologic and morphologic description of the sinus membrane-lateral bone wall complex...
November 2017: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/28985307/sequence-based-analysis-of-thermal-adaptation-and-protein-energy-landscapes-in-an-invasive-blue-mussel-mytilus-galloprovincialis
#18
Norah P Saarman, Kord M Kober, W Brian Simison, Grant H Pogson
Adaptive responses to thermal stress in poikilotherms plays an important role in determining competitive ability and species distributions. Amino acid substitutions that affect protein stability and modify the thermal optima of orthologous proteins may be particularly important in this context. Here, we examine a set of 2,770 protein-coding genes to determine if proteins in a highly invasive heat tolerant blue mussel (Mytilus galloprovincialis) contain signals of adaptive increases in protein stability relative to orthologs in a more cold tolerant M...
October 1, 2017: Genome Biology and Evolution
https://www.readbyqxmd.com/read/28967473/aluminium-induced-cell-death-requires-upregulation-of-ntvpe1-gene-coding-vacuolar-processing-enzyme-in-tobacco-nicotiana-tabacum-l
#19
Koki Kariya, Yoshiyuki Tsuchiya, Takayuki Sasaki, Yoko Yamamoto
Cell death mechanism triggered by aluminium (Al) ion was investigated at root apex of tobacco (cultivar Bright Yellow) and in cultured tobacco cell line BY-2 derived from Bright Yellow, focusing on VPE genes (NtVPE1a, NtVPE1b, NtVPE2, NtVPE3). Cell death was detected as a loss of integrity of the plasma membrane by vital staining with fluorescein diacetate (in root apex) and Evans blue (in BY-2), respectively. At root apex, the upregulation of gene expression of VPE1a and VPE1b was observed significantly after 9h of Al exposure in parallel with an enhancement of cell death, while the upregulation of VPE2 and VPE3 were observed later...
September 12, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28951802/cancer-risks-among-welders-and-occasional-welders-in-a-national-population-based-cohort-study-canadian-census-health-and-environmental-cohort
#20
Jill S MacLeod, M Anne Harris, Michael Tjepkema, Paul A Peters, Paul A Demers
BACKGROUND: Welders are exposed to many known and suspected carcinogens. An excess lung cancer risk among welders is well established, but whether this is attributable to welding fumes is unclear. Excess risks of other cancers have been suggested, but not established. We investigated welding cancer risks in the population-based Canadian Census Health and Environmental Cohort. METHODS: Among 1.1 million male workers, 12,845 welders were identified using Standard Occupational Classification codes and followed through retrospective linkage of 1991 Canadian Long Form Census and Canadian Cancer Registry (1992-2010) records...
September 2017: Safety and Health At Work
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