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https://www.readbyqxmd.com/read/28062690/cryptochrome-mediates-behavioral-executive-choice-in-response-to-uv-light
#1
Lisa S Baik, Keri J Fogle, Logan Roberts, Alexis M Galschiodt, Joshua A Chevez, Yocelyn Recinos, Vinh Nguy, Todd C Holmes
Drosophila melanogaster CRYPTOCHROME (CRY) mediates behavioral and electrophysiological responses to blue light coded by circadian and arousal neurons. However, spectroscopic and biochemical assays of heterologously expressed CRY suggest that CRY may mediate functional responses to UV-A (ultraviolet A) light as well. To determine the relative contributions of distinct phototransduction systems, we tested mutants lacking CRY and mutants with disrupted opsin-based phototransduction for behavioral and electrophysiological responses to UV light...
January 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28050362/bio-medical-waste-managment-in-a-tertiary-care-hospital-an-overview
#2
Anita Pandey, Sanjiv Ahuja, Molly Madan, Ajay Kumar Asthana
INTRODUCTION: Bio-Medical Waste (BMW) management is of utmost importance as its improper management poses serious threat to health care workers, waste handlers, patients, care givers, community and finally the environment. Simultaneously, the health care providers should know the quantity of waste generated in their facility and try to reduce the waste generation in day-to-day work because lesser amount of BMW means a lesser burden on waste disposal work and cost saving. AIM: To have an overview of management of BMW in a tertiary care teaching hospital so that effective interventions and implementations can be carried out for better outcome...
November 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28048256/su-f-t-667-development-and-validation-of-dose-calculation-for-an-open-source-kv-treatment-planning-system-for-small-animal-radiotherapy
#3
S Prajapati, X Mo, B Bednarz, M Lawless, C Hammer, R Flynn, D Westerly, R Jeraj, T Mackie
PURPOSE: An open-source, convolution/superposition based kV-treatment planning system(TPS) was developed for small animal radiotherapy from previously existed in-house MV-TPS. It is flexible and applicable to both step and shoot and helical tomotherapy treatment delivery. For initial commissioning process, the dose calculation from kV-TPS was compared with measurements and Monte Carlo(MC) simulations. METHODS: High resolution, low energy kernels were simulated using EGSnrc user code EDKnrc, which was used as an input in kV-TPS together with MC-simulated x-ray beam spectrum...
June 2016: Medical Physics
https://www.readbyqxmd.com/read/28025051/variations-in-normal-color-vision-vi-factors-underlying-individual-differences-in-hue-scaling-and-their-implications-for-models-of-color-appearance
#4
Kara J Emery, Vicki J Volbrecht, David H Peterzell, Michael A Webster
Observers with normal color vision vary widely in their judgments of color appearance, such as the specific spectral stimuli they perceive as pure or unique hues. We examined the basis of these individual differences by using factor analysis to examine the variations in hue-scaling functions from both new and previously published data. Observers reported the perceived proportion of red, green, blue or yellow in chromatic stimuli sampling angles at fixed intervals within the LM and S cone-opponent plane. These proportions were converted to hue angles in a perceptual-opponent space defined by red vs...
January 3, 2017: Vision Research
https://www.readbyqxmd.com/read/28024479/-construction-of-eukaryotic-expression-vector-of-sirna-specific-for-bcr-abl-fusion-gene-and-its-effects-on-k562-cells
#5
Ming Li, Bao-Lin Wang, Li-Na Wang, Ya-Ming Xi
OBJECTIVE: To construct eukaryotic expression vector of siRNA specific for BCR/ABL and to investigate the effect of recombinant plasmid on BCR/ABL and P210 protein expression in K562 cells. METHODS: siRNA(small interfering RNA)was designed according to the Tuschl's principle of Ai-based medicine, and was converted into cDNA coding expression of shRNA(small hairpin RNAs)of siRNA for BCR/ABL fusion gene. The cDNA was synthesized and inserted into plasmid pTER. The pTER117 and pTER363 of recombinant plasmid being eukaryotic expression vector was controlled by the H1 promoter of RNA polymerase III, and identified by the restriction map and the sequence analysis...
December 2016: Zhongguo Shi Yan Xue Ye Xue za Zhi
https://www.readbyqxmd.com/read/27959574/porcine-dorsal-root-ganglia-ovarian-neurons-are-affected-by-long-lasting-testosterone-treatment
#6
B Jana, K Palus, K Meller, J Całka
We studied the effect of testosterone overdose on the number, distribution and chemical coding of ovarian neurons in the dorsal root ganglia (DRGs) in pigs. On day 3 of the estrous cycle, the ovaries of both the control and experimental gilts were injected with retrograde tracer Fast Blue. From day 4 of the estrous cycle to the expected day 20 of the second studied cycle, the experimental gilts were injected with testosterone, while the control gilts received oil. After the completion of the protocol the Th16-L5 DRGs were collected...
December 13, 2016: Physiological Research
https://www.readbyqxmd.com/read/27934878/activating-cysteinyl-leukotriene-receptor-2-cysltr2-mutations-in-blue-nevi
#7
Inga Möller, Rajmohan Murali, Hansgeorg Müller, Thomas Wiesner, Louise A Jackett, Simone L Scholz, Ioana Cosgarea, Johannes Ap van de Nes, Antje Sucker, Uwe Hillen, Bastian Schilling, Annette Paschen, Heinz Kutzner, Arno Rütten, Martin Böckers, Richard A Scolyer, Dirk Schadendorf, Klaus G Griewank
Blue nevi are common melanocytic tumors arising in the dermal layer of the skin. Similar to uveal melanomas, blue nevi frequently harbor GNAQ and GNA11 mutations. Recently, recurrent CYSLTR2 and PLCB4 mutations were identified in uveal melanomas not harboring GNAQ or GNA11 mutations. All four genes (GNAQ, GNA11, CYSLTR2, and PLCB4) code for proteins involved in the same signaling pathway, which is activated by mutations in these genes. Given the related functional consequences of these mutations and the known genetic similarities between uveal melanoma and blue nevi, we analyzed a cohort of blue nevi to investigate whether CYSLTR2 and PLCB4 mutations occur in tumors lacking GNAQ or GNA11 mutations (as in uveal melanoma)...
December 9, 2016: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/27933209/prevalence-and-incidence-of-gout-in-southern-sweden-from-the-socioeconomic-perspective
#8
Meliha C Kapetanovic, Mohaned Hameed, Aleksandra Turkiewicz, Tuhina Neogi, Tore Saxne, Lennart Jacobsson, Martin Englund
OBJECTIVES: To estimate the prevalence and cumulative incidence of gout in southern Sweden with respect to socioeconomic status. METHODS: Among residents of Skåne region in the year 2013 (total population 1.3 million), adult persons (age 18 years +) who between 1998 and 2013 received a diagnosis of gout (International Classification of Disease 10th Edition (ICD-10) code M10) by any physician were identified using the Skåne Healthcare Register. We calculated the point prevalence by end of 2013 and annual cumulative incidence in 2013 standardised to the whole Skåne population according to sex, individual information on occupation (white collar/blue collar), income (low/middle/high) and level of education (primary school/high school/university)...
2016: RMD Open
https://www.readbyqxmd.com/read/27914729/operative-team-communication-during-simulated-emergencies-too-busy-to-respond
#9
W Austin Davis, Seth Jones, Adrianna M Crowell-Kuhnberg, Dara O'Keeffe, Kelly M Boyle, Suzanne B Klainer, Douglas S Smink, Steven Yule
BACKGROUND: Ineffective communication among members of a multidisciplinary team is associated with operative error and failure to rescue. We sought to measure operative team communication in a simulated emergency using an established communication framework called "closed loop communication." We hypothesized that communication directed at a specific recipient would be more likely to elicit a check back or closed loop response and that this relationship would vary with changes in patients' clinical status...
November 30, 2016: Surgery
https://www.readbyqxmd.com/read/27906259/spatiotemporal-blue-noise-coded-aperture-design-for-multi-shot-compressive-spectral-imaging
#10
Claudia V Correa, Henry Arguello, Gonzalo R Arce
Multi-shot coded aperture snapshot spectral imaging (CASSI) systems capture the spectral information of a scene using a small set of coded focal plane array (FPA) compressive measurements. Compressed sensing (CS) reconstruction algorithms are then used to reconstruct the underlying spectral 3D data cube from an underdetermined system of linear equations. Multiple snapshots result in a less ill-posed inverse problem and improved reconstructions. The only varying components in CASSI are the coded apertures, whose structure is crucial inasmuch as they determine the minimum number of FPA measurements needed for correct image reconstruction and the corresponding attainable quality...
December 1, 2016: Journal of the Optical Society of America. A, Optics, Image Science, and Vision
https://www.readbyqxmd.com/read/27895772/hotair-a-long-non-coding-rna-driver-of-malignancy-whose-expression-is-activated-by-foxc1-negatively-regulates-mirna-1-in-hepatocellular-carcinoma
#11
Dong-Na Su, Shi-Pin Wu, Hong-Tao Chen, Jin-Hua He
Evidence is rapidly accumulating that long non-coding RNAs (lncRNAs) are involved in human tumorigenesis and are dysregulated in multiple cancers, including hepatocellular carcinoma (HCC). lncRNAs can regulate essential pathways that contribute to tumor initiation and progression with tissue specificity, which suggests that lncRNAs may be valuable biomarkers and therapeutic targets. HOX transcript antisense intergenic RNA (HOTAIR) has previously been demonstrated to be an oncogene and a negative prognostic factor in a variety of cancers; however, the factors that contribute to the upregulation of HOTAIR and the interaction between HOTAIR and microRNAs (miRNAs or miRs) are largely unknown...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27887719/leucine-rich-repeat-containing-g-protein-coupled%C3%A2-receptor%C3%A2-5-positive%C3%A2-cells%C3%A2-in-the-endometrial-stem-cell-niche
#12
Irene Cervelló, Claudia Gil-Sanchis, Xavier Santamaría, Amparo Faus, Julia Vallvé-Juanico, Patricia Díaz-Gimeno, Oriana Genolet, Antonio Pellicer, Carlos Simón
OBJECTIVE: To study, isolate and characterize leucine-rich repeat-containing heterotrimeric guanine nucleotide-binding protein-coupled receptor 5 (LGR5)-positive cells from human endometrium to determine their functional relevance. DESIGN: Prospective experimental animal study. SETTING: University research laboratories. ANIMAL(S): Nonobese diabetic mice (NOD-SCID) (strain code 394; NOD.CB17-Prkdc(scid)/NcrCrl). INTERVENTION(S): Human LGR5(+) cells were labeled with superparamagnetic iron oxide nanoparticles (SPIOs) and injected under the kidney capsule in immunocompromised mice...
November 22, 2016: Fertility and Sterility
https://www.readbyqxmd.com/read/27871884/structural-characterization-of-recombinant-human-fibroblast-growth-factor-receptor-2b-kinase-domain-upon-interaction-with-omega-fatty-acids
#13
Masoumeh Moghadasi, Dariush Ilghari, Majid Sirati-Sabet, Abbas Amini, Hamideh Asghari, Nematollah Gheibi
The mutated recombinant kinase domain of human fibroblast growth factor receptor 2b (hFGFR2b) is overexpressed and purified, and its structural changes upon the interaction with three unsaturated fatty acids (UFAs), oleic, linoleic and α-linolenic are studied. This interaction is investigated to find out about the folding and unfolding effect of unsaturated fatty acids on the kinase domain structure of hFGFR2b. Recombinant pLEICS-01 vectors, containing the mutated coding region of hFGFR2b, are expressed in the standard Escherichia coli BL21 (DE3) host cells and purified by Ni(2+)-NTA affinity chromatography...
November 18, 2016: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/27866657/distribution-and-chemical-coding-of-sensory-neurons-innervating-the-skin-of-the-porcine-hindlimb
#14
Anna Kozłowska, Anita Mikołajczyk, Zbigniew Adamiak, Mariusz Majewski
The aim of the present study was to establish the origin and chemical phenotyping of neurons involved in skin innervation of the porcine hind leg. The dorsal root ganglia (DRGs) of the lumbar (L4-L6) and sacral (S1-S3) spinal nerves were visualized using the fluorescent tracer Fast Blue (FB). The morphometric analysis of FB-positive (FB+)neurons showed that in the L4, L5, S1 and S2 DRGs, the small-sized perikarya constituted the major population, whereas in the L6 and S3 DRGs the medium-sized cells made up the major population...
November 5, 2016: Neuropeptides
https://www.readbyqxmd.com/read/27866227/genome-wide-association-mapping-of-resistance-to-eyespot-disease-pseudocercosporella-herpotrichoides-in-european-winter-wheat-triticum-aestivum-l-and-fine-mapping-of-pch1
#15
Christine D Zanke, Bernd Rodemann, Jie Ling, Quddoos H Muqaddasi, Jörg Plieske, Andreas Polley, Sonja Kollers, Erhard Ebmeyer, Viktor Korzun, Odile Argillier, Gunther Stiewe, Thomas Zschäckel, Martin W Ganal, Marion S Röder
Genotypes with recombination events in the Triticum ventricosum introgression on chromosome 7D allowed to fine-map resistance gene Pch1, the main source of eyespot resistance in European winter wheat cultivars. Eyespot (also called Strawbreaker) is a common and serious fungal disease of winter wheat caused by the necrotrophic fungi Oculimacula yallundae and Oculimacula acuformis (former name Pseudocercosporella herpotrichoides). A genome-wide association study (GWAS) for eyespot was performed with 732 microsatellite markers (SSR) and 7761 mapped SNP markers derived from the 90 K iSELECT wheat array using a panel of 168 European winter wheat varieties as well as three spring wheat varieties and phenotypic evaluation of eyespot in field tests in three environments...
November 19, 2016: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
https://www.readbyqxmd.com/read/27850859/1223-less-than-half-of-inpatient-code-blue-activations-are-for-cardiopulmonary-arrest
#16
Julian Villar, Majid Shafiq, Lijia Xie, Paul Mohabir
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27850077/437-evaluation-of-code-blue-simulation-on-interprofessional-competency-among-pharmacy-students
#17
Marian Gaviola, Meredith Howard, Randy Martin
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27850014/374-does-nurse-practitioner-experience-level-affect-differential-diagnosis-generation-during-code-blue
#18
Courtney Coker, Maren Wiseheart, Lizzy Wooley, John Watson, Ginger Tsai-Nguyen, Adan Mora
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27850000/360-a-university-hospital-review-of-code-blue-a-journey-toward-improvement
#19
Renee Hebbeler-Clark, Ann Schlinkert
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
https://www.readbyqxmd.com/read/27849994/354-does-code-blue-simulation-training-help-nurse-practitioners-identify-members-of-code-team
#20
Courtney Coker, John Watson, Lizzy Wooley, Maren Wiseheart, Ginger Tsai-Nguyen, Adan Mora
No abstract text is available yet for this article.
December 2016: Critical Care Medicine
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