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Saliva biology

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https://www.readbyqxmd.com/read/29240901/daily-social-interactions-and-the-biological-stress-response-are-there-age-differences-in-links-between-social-interactions-and-alpha-amylase
#1
Kira S Birditt, Lauren A Tighe, Michael R Nevitt, Steven H Zarit
Background and Objectives: According to the strength and vulnerability integration (SAVI) model, older people are better able to avoid negative social interactions than younger people, but when they do experience negative interactions, they are equally or more emotionally and physiologically reactive than younger people. Less is known about the links between daily negative and positive social encounters and the sympathetic adrenal medullary system (a key stress pathway) and whether there are age differences in these links...
December 12, 2017: Gerontologist
https://www.readbyqxmd.com/read/29238879/the-small-vesicular-culprits-the-investigation-of-extracellular-vesicles-as-new-targets-for-cancer-treatment
#2
REVIEW
Fumihiko Urabe, Nobuyoshi Kosaka, Yusuke Yoshioka, Shin Egawa, Takahiro Ochiya
Extracellular vesicles (EVs) are membranous vesicles released from almost all type of cells including cancer cells. EVs transfer their components, such as microRNAs (miRNAs), messenger RNAs, lipids and proteins, from one cell to another, affecting the target cells. Emerging evidence suggests that reciprocal interactions between cancer cells and the cells in their microenvironment via EVs drive disease progression and therapy resistance. Therefore, understanding the roles of EVs in cancer biology will provide us with new opportunities to treat patients...
December 13, 2017: Clinical and Translational Medicine
https://www.readbyqxmd.com/read/29236088/dicationic-imidazolium-based-ionic-liquid-coatings-on-zirconia-surfaces-physico-chemical-and-biological-characterization
#3
Pavan P K Sandhu, Izabelle M Gindri, Danyal A Siddiqui, Danieli C Rodrigues
In the present work, dicationic imidazolium-based ionic liquids (ILs) were investigated as multi-functional coatings on a zirconia (ZrO₂) surface to prevent biofilm formation and enhance the wear performance of zirconia while maintaining the material's compatibility with host cells. ILs containing phenylalanine and methionine were synthesized and deposited on zirconia. Intermolecular interactions driving IL deposition on zirconia were studied using X-ray photoelectron spectroscopy (XPS). Anti-biofilm activity and cell compatibility were evaluated in vitro after one and seven days, and wear performance was tested using a pin-on-disk apparatus...
December 13, 2017: Journal of Functional Biomaterials
https://www.readbyqxmd.com/read/29231025/-comparison-of-mpure-12-automatic-nucleic-acid-purification-and-chelex-100-method
#4
X Shen, M Li, Y L Wang, Y L Chen, Y Lin, Z M Zhao, T Z Que
OBJECTIVES: To explore the forensic application value of MPure-12 automatic nucleic acid purification (MPure-12 Method) for DNA extraction by extracting and typing DNA from bloodstains and various kinds of biological samples with different DNA contents. METHODS: Nine types of biological samples, such as bloodstains, semen stains, and saliva were collected. DNA were extracted using MPure-12 method and Chelex-100 method, followed by PCR amplification and electrophoresis for obtaining STR-profiles...
April 2017: Fa Yi Xue za Zhi
https://www.readbyqxmd.com/read/29228383/oral-fungal-bacterial-biofilm-models-in-vitro-a-review
#5
Marlene Chevalier, Stephane Ranque, Isabelle Prêcheur
Inclusion of fungi as commensals in oral biofilm is an important innovation in oral biology, and this work aimed to review the literature on the available biofilm and related disease in vitro models. Actually, thousands of bacterial and around one hundred of fungal phylotypes can colonize the oral cavity. Taxonomic profiling combined with functional expression analysis has revealed that Candida albicans, Streptococcus mutans and prominent periodontopathogens are not always present or numerically important in candidiasis, caries, or periodontitis lesions...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29225778/zika-virus-reservoirs-implications-for-transmission-future-outbreaks-drug-and-vaccine-development
#6
REVIEW
Raj Kalkeri, Krishna K Murthy
Zika virus (ZIKV) was recently declared as a 'Global Health Emergency' by the World Health Organization. Various tissue reservoirs of ZIKV in infected humans and animals models have been observed, the implications of which are not known. Compared to other Flaviviruses, sexual transmission and persistence in the genitourinary tract seem to be unique to ZIKV. ZIKV persistence and shedding in bodily secretions (e.g. saliva, semen) is a concern for potential disease spread and could pose challenges in diagnosis, regulatory guidelines and drug/vaccine development...
2017: F1000Research
https://www.readbyqxmd.com/read/29225100/controllable-encapsulation-of-%C3%AE-mangostin-with-quaternized-%C3%AE-cyclodextrin-grafted-chitosan-using-high-shear-mixing
#7
Sarunya Phunpee, Kunat Suktham, Suvimol Surassmo, Suwatchai Jarussophon, Chompoonut Rungnim, Apinan Soottitantawat, Satit Puttipipatkhachorn, Uracha Rungsardthong Ruktanonchai
In this study, the inclusion complex formation between α-mangostin and water-soluble quaternized β-CD grafted-chitosan (QCD-g-CS) was investigated. Inclusion complex formation with encapsulation efficiency (%EE) of 5, 15 and 75% can be varied using high speed homogenizer. Tuning %EE plays a role on physicochemical and biological properties of α-mangostin/QCD-g-CS complex. Molecular dynamics simulations indicate that α-mangostin is included within the hydrophobic β-CD cavity and being absorbed on the QCD-g-CS surface, with these results being confirmed by Fourier transform infrared (FTIR) spectroscopy...
December 7, 2017: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29218397/can-we-better-predict-the-biologic-behavior-of-incidental-ipmn-a-comprehensive-analysis-of-molecular-diagnostics-and-biomarkers-in-intraductal-papillary-mucinous-neoplasms-of-the-pancreas
#8
Kiara A Tulla, Ajay V Maker
PURPOSE: Predicting the biologic behavior of intraductal papillary mucinous neoplasm (IPMN) remains challenging. Current guidelines utilize patient symptoms and imaging characteristics to determine appropriate surgical candidates. However, the majority of resected cysts remain low-risk lesions, many of which may be feasible to have under surveillance. We herein characterize the most promising and up-to-date molecular diagnostics in order to identify optimal components of a molecular signature to distinguish levels of IPMN dysplasia...
December 7, 2017: Langenbeck's Archives of Surgery
https://www.readbyqxmd.com/read/29216831/validity-of-mobile-electronic-data-capture-in-clinical-studies-a-pilot-study-in-a-pediatric-population
#9
Belinda von Niederhäusern, Ramon Saccilotto, Sabine Schädelin, Victoria Ziesenitz, Pascal Benkert, Marie-Luise Decker, Anya Hammann, Julia Bielicki, Marc Pfister, Christiane Pauli-Magnus
BACKGROUND: Clinical studies in children are necessary yet conducting multiple visits at study centers remains challenging. The success of "care-at-home" initiatives and remote clinical trials suggests their potential to facilitate conduct of pediatric studies. This pilot aimed to study the feasibility of remotely collecting valid (i.e. complete and correct) saliva samples and clinical data utilizing mobile technology. METHODS: Single-center, prospective pilot study in children undergoing elective tonsillectomy at the University of Basel Children's Hospital...
December 8, 2017: BMC Medical Research Methodology
https://www.readbyqxmd.com/read/29215915/perspectives-on-the-relevance-of-the-circadian-time-structure-to-workplace-threshold-limit-values-and-employee-biological-monitoring
#10
Michael H Smolensky, Alain E Reinberg, Linda Sackett-Lundeen
The circadian time structure (CTS) and its disruption by rotating and nightshift schedules relative to work performance, accident risk, and health/wellbeing have long been areas of occupational medicine research. Yet, there has been little exploration of the relevance of the CTS to setting short-term, time-weighted, and ceiling threshold limit values (TLVs); conducting employee biological monitoring (BM); and establishing normative reference biological exposure indices (BEIs). Numerous publications during the past six decades document the CTS substantially affects the disposition - absorption, distribution, metabolism, and elimination - and effects of medications...
December 7, 2017: Chronobiology International
https://www.readbyqxmd.com/read/29201965/salivary-chemical-factors-in-relation-with-oral-cancer-in-smokers-and-non-smokers-a-literature-review
#11
REVIEW
Tahereh Nosratzehi
Squamous cell carcinoma of oral cavity is of malignant tumors, which causes cancerous complications. DNA damage, mainly because of products of oxidative stress like reactive oxygen species, is a frequent mutagenic that triggers carcinoma. Smoking increases the probability of cancer incidence. Saliva is the first biological medium to interact with external compounds, especially smoking substances. The present study overviews the salivary level of some remarkable compounds in relation with smoking and squamous cell carcinoma...
December 2017: Journal of Dentistry
https://www.readbyqxmd.com/read/29199151/gender-influence-on-the-salivary-protein-profile-of-finishing-pigs
#12
Ana M Gutiérrez, Ana Montes, Cándido Gutiérrez-Panizo, Pablo Fuentes, Ernesto De La Cruz-Sánchez
A study on gender differences in the normal range of biomarkers in porcine saliva samples has the scope for further attention. In the present study, the salivary protein profiles of age-matched healthy male and female finishing pigs were compared. The levels of salivary adenosine deaminase (ADA) activity, haptoglobin (Hp) and C-reactive protein (CRP) have been quantified in 32 male and 32 female pigs to ensure the presence of gender effect on the median levels of salivary biomarkers. Moreover, the total salivary protein content was quantified and compared...
November 30, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29181457/comparison-of-real-time-quantitative-pcr-with-a-chairside-test-for-streptococcus-mutans-assessment
#13
Yihong Li, Prakaimuk Saraithong, Zhou Chen, Erica Leung, Komkham Pattanaporn, Ananda Dasanayake
OBJECTIVE: To compare two methods for Streptococcus mutans detection and quantification in the human oral cavity: a chairside commercial test and a molecular-based real-time quantitative polymerase chain reaction (qPCR) method. METHODS: A total of 688 whole saliva samples were collected from 344 children aged 3 and 5 and their biological mothers. Caries status was examined using a World Health Organisation survey method. S. mutans levels were measured using the Dentocult SM Strip mutans test and scored as colony forming units per millilitre of saliva...
2017: Chinese Journal of Dental Research
https://www.readbyqxmd.com/read/29178733/the-role-of-adhesive-materials-and-oral-biofilm-in-the-failure-of-adhesive-resin-restorations
#14
REVIEW
Roberto Pinna, Paolo Usai, Enrica Filigheddu, Franklin Garcia-Godoy, Egle Milia
PURPOSE: To critically discuss adhesive materials and oral cariogenic biofilm in terms of their potential relevance to the failures of adhesive restorations in the oral environment. METHODS: The literature regarding adhesive restoration failures was reviewed with particular emphasis on the chemistry of adhesive resins, weakness in dentin bonding, water fluids, cariogenic oral biofilm and the relations that influence failures. Particular attention was paid to evidence derived from clinical studies...
October 2017: American Journal of Dentistry
https://www.readbyqxmd.com/read/29169777/one-step-fplc-size-exclusion-chromatography-procedure-for-purification-of-rdmbt1-6%C3%A2-kb-with-increased-biological-activity
#15
Martina Tuttolomondo, Pernille Lund Hansen, Jan Mollenhauer, Henrik J Ditzel
Deleted in Malignant Brain Tumor 1 (DMBT1, alias SAG or gp340) is a pattern recognition receptor involved in immune defense, cell polarization, differentiation and regeneration. To investigate the role of the protein in physiological and pathological processes, the protein has often been isolated from saliva or produced in vitro and purified by a multistep affinity purification procedure using bacteria, followed by FPLC. Here, we compared a simple, one-step FPLC-SEC protocol for purification of recombinant DMBT1 6 kb, with that of the standard bacteria affinity purification-based protocol...
November 20, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29168023/oxytocin-and-parenting-behavior-among-impoverished-mothers-with-low-vs-high-early-life-stress
#16
Megan M Julian, Katherine L Rosenblum, Jenalee R Doom, Christy Y Y Leung, Julie C Lumeng, Michelle Gómez Cruz, Delia M Vazquez, Alison L Miller
Recent work suggests that key aspects of sensitive parenting (e.g., warmth, emotional attunement) may be shaped in part by biology, specifically the neuropeptide oxytocin. However, some studies have found that oxytocin may not act in expected ways in higher-risk populations (e.g., those with postnatal depression or borderline personality disorder). This study examined the relation between oxytocin and parenting among mothers with varying levels of early life stress. Forty low-income mothers and their 34- to 48-month-old child participated in this study...
November 22, 2017: Archives of Women's Mental Health
https://www.readbyqxmd.com/read/29165156/total-arsenic-and-speciation-analysis-of-saliva-and-urine-samples-from-individuals-living-in-a-chronic-arsenicosis-area-in-china
#17
Dapeng Wang, Yasuyo Shimoda, Sanxiang Wang, Zhenghui Wang, Jian Liu, Xing Liu, Huanyu Jin, Fenfang Gao, Jian Tong, Kenzo Yamanaka, Jie Zhang, Yan An
BACKGROUND: It is generally acknowledged that the determination of harmful chemical compounds excreted into saliva is useful for assessing their exposure levels. The aim of the present study was to compare the total arsenic and its species in saliva and urine samples collected from the people residing in an arsenic-contaminated area of China and to further verify the feasibility of using salivary arsenic as a new biomarker of arsenic exposure. METHODS: Total arsenic and speciation analyses in urine and saliva samples among 70 residents exposed to arsenic from drinking water in Shanxi, China were carried out by high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP/MS)...
May 11, 2017: Environmental Health and Preventive Medicine
https://www.readbyqxmd.com/read/29164067/tick-borne-transmission-of-murine-gammaherpesvirus-68
#18
Valeria Hajnická, Marcela Kúdelová, Iveta Štibrániová, Mirko Slovák, Pavlína Bartíková, Zuzana Halásová, Peter Pančík, Petra Belvončíková, Michaela Vrbová, Viera Holíková, Rosemary S Hails, Patricia A Nuttall
Herpesviruses are a large group of DNA viruses infecting mainly vertebrates. Murine gammaherpesvirus 68 (MHV68) is often used as a model in studies of the pathogenesis of clinically important human gammaherpesviruses such as Epstein-Barr virus and Kaposi's sarcoma-associated herpesvirus. This rodent virus appears to be geographically widespread; however, its natural transmission cycle is unknown. Following detection of MHV68 in field-collected ticks, including isolation of the virus from tick salivary glands and ovaries, we investigated whether MHV68 is a tick-borne virus...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29163938/understanding-biological-mechanisms-underlying-adverse-birth-outcomes-in-developing-countries-protocol-for-a-prospective-cohort-amanhi-bio-banking-study
#19
Abdullah H Baqui, Rasheda Khanam, Mohammad Sayedur Rahman, Aziz Ahmed, Hasna Hena Rahman, Mamun Ibne Moin, Salahuddin Ahmed, Fyezah Jehan, Imran Nisar, Atiya Hussain, Muhammad Ilyas, Aneeta Hotwani, Muhammad Sajid, Shahida Qureshi, Anita Zaidi, Sunil Sazawal, Said M Ali, Saikat Deb, Mohammed Hamad Juma, Usha Dhingra, Arup Dutta, Shaali Makame Ame, Caroline Hayward, Igor Rudan, Mike Zangenberg, Donna Russell, Sachiyo Yoshida, Ozren Polašek, Alexander Manu, Rajiv Bahl
Objectives: The AMANHI study aims to seek for biomarkers as predictors of important pregnancy-related outcomes, and establish a biobank in developing countries for future research as new methods and technologies become available. Methods: AMANHI is using harmonised protocols to enrol 3000 women in early pregnancies (8-19 weeks of gestation) for population-based follow-up in pregnancy up to 42 days postpartum in Bangladesh, Pakistan and Tanzania, with collection taking place between August 2014 and June 2016...
December 2017: Journal of Global Health
https://www.readbyqxmd.com/read/29162170/dynamic-stress-related-epigenetic-regulation-of-the-glucocorticoid-receptor-gene-promoter-during-early-development-the-role-of-child-maltreatment
#20
Justin Parent, Stephanie H Parade, Laura E Laumann, Kathryn K Ridout, Bao-Zhu Yang, Carmen J Marsit, Ronald Seifer, Audrey R Tyrka
Epigenetics processes may play a vital role in the biological embedding of early environmental adversity and the development of psychopathology. Accumulating evidence suggests that maltreatment is linked to methylation of the glucocorticoid receptor gene, nuclear receptor subfamily 3, group C, member 1 (NR3C1), which is a key regulator of the hypothalamus-pituitary-adrenal axis. However, prior work has been exclusively cross-sectional, greatly constraining our understanding of stress-related epigenetic processes over time...
December 2017: Development and Psychopathology
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