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Saliva biology

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https://www.readbyqxmd.com/read/28444531/multifaceted-sexual-desire-and-hormonal-associations-accounting-for-social-location-relationship-status-and-desire-target
#1
Sara B Chadwick, Shannon M Burke, Katherine L Goldey, Sari M van Anders
Sexual desire is typically measured as a unitary erotic phenomenon and is often assumed by biological and biomedical researchers, as well as the lay public, to be directly connected to physiological parameters like testosterone (T). In the present study, we empirically examined how conceptualizing sexual desire as multifaceted might clarify associations with T and contextual variables. To do so, we used the Sexual Desire Questionnaire (DESQ), which assesses multifaceted dyadic sexual desire, to explore how contextual variables such as social location, relationship status, and desire target (e...
April 25, 2017: Archives of Sexual Behavior
https://www.readbyqxmd.com/read/28444055/prospects-of-studies-on-violence-adolescence-and-cortisol-a-systematic-literature-review
#2
Leonardo Planel Lugarinho, Joviana Quintes Avanci, Liana Wernersbach Pinto
Violence has a negative impact on adolescents and affects their quality of life. It causes stress and requires the victim's adaptive capacity, which can cause psychological and biological changes. Hormone cortisol levels have been used as stress biomarker in several studies. This paper aims to perform a systematic literature review of publications on cortisol and violence involving teenagers from 2000 to 2013. Descriptors "cortisol", "violence" and "adolescent" were used in both English and Portuguese in this review, which included bibliographic databases PubMed/Medline, Lilacs, BVS and SciELO...
April 2017: Ciência & Saúde Coletiva
https://www.readbyqxmd.com/read/28441039/high-throughput-processing-to-preserve-viable-cells-a-precision-medicine-initiative-cohort-program-workshop
#3
Rebekah S Rasooly, Daniel R Gossett, Marianne K Henderson, Allison Hubel, Stephen N Thibodeau
Conventionally, biobanks supporting clinical research studies have preserved serum, plasma, urine, saliva, a variety of tissue types, and stool. With the emergence of increasingly sophisticated technologies for analyzing single cells, there is growing interest in preserving viable blood cells for future functional studies. The new All of Us Research Program (formerly the Precision Medicine Initiative Cohort Program) biobank plans to house samples from a million or more individuals as part of a cohort with rich phenotypic data and longitudinal follow-up ( www...
April 25, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28435894/oral-bacterial-acid-base-metabolism-in-caries-screening-a-proof-of-concept-study
#4
E Morou-Bermudez, M A Loza-Herrero, V Garcia-Rivas, E Suarez-Perez, R J Billings
The objective of this cross-sectional study was to clinically validate an array of biochemical tests for oral acid/alkali generation as caries screening instruments. 185 adult subjects (mean 33.6±10.6 years) were examined clinically for dental caries using the ICDAS criteria. Bitewing radiographs were used to confirm interproximal surfaces of posterior teeth. For the purposes of this study, subjects were classified as "caries-active" if they had at least one untreated caries lesion with ICDAS 4 or higher. Pooled supragingival plaque and unstimulated saliva samples were collected and assayed for pH changes from sucrose and urea metabolism using colorimetric tests...
April 2017: JDR Clinical and Translational Research
https://www.readbyqxmd.com/read/28419912/the-mucosal-pellicle-an-underestimated-factor-in-oral-physiology
#5
REVIEW
Christian Hannig, Matthias Hannig, Anna Kensche, Guy Carpenter
Bioadhesion and bio-adsorption of proteins, glycoproteins and other biomolecules are ubiquitous phenomena in the oral cavity. While the protective role of the adsorbed salivary biomolecules on teeth (the acquired enamel pellicle) is well established, it has yet to be defined whether comparable processes occur on the desquamating oral soft tissues. The general term for these layers is pellicle, but due to the different characteristics of the coated surfaces the enamel pellicle and mucosal pellicle are their own entities...
April 8, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28411827/sensitive-determination-of-malondialdehyde-in-exhaled-breath-condensate-and-biological-fluids-by-capillary-electrophoresis-with-laser-induced-fluorescence-detection
#6
Júlia Lačná, František Foret, Petr Kubáň
In this work, a sensitive capillary electrophoresis method with laser induced fluorescence detection for determination of malondialdehyde in various biological fluids was developed. Malondialdehyde reacts with thiobarbituric acid under optimized conditions of pH=2, reaction time of 60min and temperature of 90°C, yielding an adduct that can be separated in a 50mM sodium borate background electrolyte at pH 9. The separation of the formed adduct was accomplished in less than 6min with limit of detection of 1...
July 1, 2017: Talanta
https://www.readbyqxmd.com/read/28411626/quantitative-determination-of-polysulfide-in-albumins-plasma-proteins-and-biological-fluid-samples-using-a-novel-combined-assays-approach
#7
Mayumi Ikeda, Yu Ishima, Akitomo Shibata, Victor T G Chuang, Tomohiro Sawa, Hideshi Ihara, Hiroshi Watanabe, Ming Xian, Yuya Ouchi, Taro Shimizu, Hidenori Ando, Masami Ukawa, Tatsuhiro Ishida, Takaaki Akaike, Masaki Otagiri, Toru Maruyama
Hydrogen sulfide (H2S) signaling involves polysulfide (RSSnSR') formation on various proteins. However, the current lack of sensitive polysulfide detection assays poses methodological challenges for understanding sulfane sulfur homeostasis and signaling. We developed a novel combined assay by modifying Sulfide Antioxidant Buffer (SAOB) to produce an "Elimination Method of Sulfide from Polysulfide" (EMSP) treatment solution that liberates sulfide, followed with methylene blue (MB) sulfide detection assay. The combined EMSP-MB sulfide detection assay performed on low molecular weight sulfur species showed that sulfide was produced from trisulfide compounds such as glutathione trisulfide and diallyl trisulfide, but not from the thiol compounds such as cysteine, cystine and glutathione...
May 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28411466/capillary-isoelectric-focusing-with-whole-column-imaging-detection-icief-a-new-approach-to-the-characterization-and-quantification-of-salivary-%C3%AE-amylase
#8
Atefeh S Zarabadi, Tiemin Huang, John G Mielke
Saliva is an easily collected biological fluid with potentially important diagnostic value. While gel electrophoresis is generally used for salivary analysis, we employed the capillary isoelectric focusing technique to allow for a rapid, automated mode of electrophoresis. Capillary isoelectric focusing coupled with UV whole column imaging detection (iCIEF) was used to develop a robust protocol to characterize salivary α-amylase collected from various glands. Notably, three sample preparation methods were examined: ultrafiltration, gel-filtration, and starch affinity interaction with salivary amylase...
March 30, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28411412/individual-differences-in-the-activity-of-the-hypothalamic-pituitary-adrenal-axis-relations-to-age-and-cumulative-risk-in-early-childhood
#9
Steven J Holochwost, Jean-Louis Gariépy, W Roger Mills-Koonce, Cathi B Propper, Jacek Kolacz, Douglas A Granger
This study examined individual differences in the function of the hypothalamic-pituitary-adrenal (HPA) axis with regard to age and cumulative risk during challenging laboratory tasks administered at 6, 12, 24, and 36 months. Saliva samples were collected from a majority-minority sample of N=185 children (57% African American, 50% female) prior to and following these tasks and later assayed for cortisol. Cumulative distal risk was indexed via a composite of maternal marital status, maternal education, income-to-needs ratio, the number of children in the household, and maternal age at childbirth...
March 24, 2017: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/28410618/liquid-biopsy-a-step-forward-towards-precision-medicine-in-urologic-malignancies
#10
REVIEW
Ashley Di Meo, Jenni Bartlett, Yufeng Cheng, Maria D Pasic, George M Yousef
There is a growing trend towards exploring the use of a minimally invasive "liquid biopsy" to identify biomarkers in a number of cancers, including urologic malignancies. Multiple aspects can be assessed in circulating cell-free DNA, including cell-free DNA levels, integrity, methylation and mutations. Other prospective liquid biopsy markers include circulating tumor cells, circulating RNAs (miRNA, lncRNAs and mRNAs), cell-free proteins, peptides and exosomes have also emerged as non-invasive cancer biomarkers...
April 14, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28410004/rna-in-salivary-extracellular-vesicles-as-a-possible-tool-for-systemic-disease-diagnosis
#11
J Kim, H Shin, J Park
Saliva contains biological information as blood and is recognized as a valuable diagnostic medium for their noninvasiveness. Although "-omics" researches have tried to investigate saliva, the origin and significance of its contents are not clear, and its usage is largely confined to oral disease in the diagnostic and prognostic field. In an attempt to broaden the applicability of saliva and to find systemic disease-derived RNA in saliva, we made mouse models that had human melanoma and isolated extracellular vesicles (EVs) from their saliva by an aqueous 2-phase system (ATPS), then identified and evaluated their expression of human melan-A RNA, which is associated with melanoma on skin...
April 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28405258/rapid-isolation-of-blood-plasma-using-a-cascaded-inertial-microfluidic-device
#12
M Robinson, H Marks, T Hinsdale, K Maitland, G Coté
Blood, saliva, mucus, sweat, sputum, and other biological fluids are often hindered in their ability to be used in point-of-care (POC) diagnostics because their assays require some form of off-site sample pre-preparation to effectively separate biomarkers from larger components such as cells. The rapid isolation, identification, and quantification of proteins and other small molecules circulating in the blood plasma from larger interfering molecules are therefore particularly important factors for optical blood diagnostic tests, in particular, when using optical approaches that incur spectroscopic interference from hemoglobin-rich red blood cells (RBCs)...
March 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/28403353/periodontitis-and-type-2-diabetes-among-women-with-previous-gestational-diabetes-epidemiological-and-immunological-aspects-in-a-follow-up-of-three-years
#13
Rafael Paschoal Esteves Lima, Luis Otávio Miranda Cota, Tarcília Aparecida Silva, Sheila Cavalca Cortelli, José Roberto Cortelli, Fernando Oliveira Costa
Objective: The aim of this study was to verify the incidence on the development of type 2 diabetes in women with previous gestational diabetes with and without periodontitis after a three-year time interval. Material and Methods: Initial sample of this follow-up study consisted of 90 women diagnosed with gestational diabetes who underwent periodontal examination. After three years, 49 women were subjected to new periodontal examination and biological, behavioral, and social data of interest were collected...
March 2017: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/28387949/mindfulness-practice-reduces-cortisol-blunting-during-chemotherapy-a-randomized-controlled-study-of-colorectal-cancer-patients
#14
David S Black, Cheng Peng, Alix G Sleight, Nathalie Nguyen, Heinz-Josef Lenz, Jane C Figueiredo
BACKGROUND: The objective of this randomized clinical experiment was to test the influence of a mindfulness meditation practice, when delivered during 1 session of active chemotherapy administration, on the acute salivary cortisol response as a marker of neuroendocrine system activity in cancer patients. METHODS: A mindfulness, attention-control, or resting exposure was assigned to 57 English- or Spanish-speaking colorectal cancer patients at 1 county oncology clinic and 1 university oncology clinic at the start of chemotherapy...
April 7, 2017: Cancer
https://www.readbyqxmd.com/read/28384983/association-study-of-vitamin-d-receptor-vdr-related-genetic-polymorphisms-and-their-haplotypes-with-chronic-periodontitis-in-colombian-population
#15
Sergio Iván Tobón-Arroyave, Diana María Isaza-Guzmán, Nicolás Pineda-Trujillo
INTRODUCTION: There is strong evidence that both genetic and environmental factors may affect the periodontal clinical status. However, epidemiological evidence on the association between Vitamin D Receptor (VDR) polymorphisms and Chronic Periodontitis (CP) has been inconsistent. AIM: The focus of this study was to identify if a possible association between VDR Single-Nucleotide Polymorphisms (SNPs) may be implicated in the aetiopathogenesis of CP in Colombian population...
February 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28382711/prevalence-of-premastication-among-children-aged-6-36%C3%A2-months-and-its-association-with-health-a-cross-sectional-study-in-eight-cities-of-china
#16
Ai Zhao, Wei Zheng, Yong Xue, Hao Li, Shengjie Tan, Wenzhi Zhao, Peiyu Wang, Yumei Zhang
Premastication is thought to be an adaptive behavior in the introduction of complementary plant-bassed food to infants. It arouses controversy, however, because of the potential for transmitting saliva-born infectious diseases. The aim of this study was to explore whether premastication by healthy caregivers was associated with children's health and behavior. The data were collected as part of the Maternal and Infant Nutrition and Growth study. From 8 cities in China, 1341 pairs of infants/toddlers and their caregivers were recruited...
April 6, 2017: Maternal & Child Nutrition
https://www.readbyqxmd.com/read/28367959/polymorphisms-of-sts-gene-and-sult2a1-gene-and-neurosteroid-levels-in-han-chinese-boys-with-attention-deficit-hyperactivity-disorder-an-exploratory-investigation
#17
Liang-Jen Wang, Wen-Ching Chan, Miao-Chun Chou, Wen-Jiun Chou, Min-Jing Lee, Sheng-Yu Lee, Pao-Yen Lin, Yi-Hsin Yang, Cheng-Fang Yen
This study examined the relationships among polymorphisms of the STS gene and SULT2A1 gene, dehydroepiandrosterone (DHEA) and its sulfated form (DHEA-S), and characteristics of attention-deficit/hyperactivity disorder (ADHD). We used cheek swabs to obtain the genomic DNA of 200 ADHD male probands (mean age: 8.7 years), 192 patients' mothers and 157 patients' fathers. Three SNPs in the STS gene (rs6639786, rs2270112, and rs17268988) and one SNP in the SULT2A1 gene (rs182420) were genotyped. Saliva samples were collected from the ADHD patients to analyze DHEA and DHEA-S levels...
April 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28351566/micrornas-in-human-tongue-squamous-cell-carcinoma-from-pathogenesis-to-therapeutic-implications
#18
REVIEW
Omer Faruk Karatas, Muhammet Oner, Alican Abay, Ali Diyapoglu
Being one of the most aggressive cancers of oral cavity, tongue squamous cell carcinoma (TSCC) constitutes 41% of all oral carcinomas. Despite considerable improvements in multimodal diagnosis and treatment techniques, TSCC still remains to be one of the most lethal cancer types in the head and neck region. MicroRNAs are endogenously synthesized, small, non-coding RNAs, which are responsible for post-transcriptional regulation of mRNA expression. They are involved in regulation of almost all biological processes through their spatial and temporal expression...
April 2017: Oral Oncology
https://www.readbyqxmd.com/read/28346122/non-targeted-metabolomics-in-sport-and-exercise-science
#19
Liam M Heaney, Kevin Deighton, Toru Suzuki
Metabolomics incorporates the study of metabolites that are produced and released through physiological processes at both the systemic and cellular levels. Biological compounds at the metabolite level are of paramount interest in the sport and exercise sciences, although research in this field has rarely been referred to with the global 'omics terminology. Commonly studied metabolites in exercise science are notably within cellular pathways for adenosine triphosphate production such as glycolysis (e.g., pyruvate and lactate), β-oxidation of free fatty acids (e...
March 27, 2017: Journal of Sports Sciences
https://www.readbyqxmd.com/read/28343672/effect-of-titanium-and-zirconia-dental-implant-abutments-on-a-cultivable-polymicrobial-saliva-community
#20
Erica D de Avila, Carlos Eduardo Vergani, Francisco A Mollo Junior, Miguel Jafelicci Junior, Wenyuan Shi, Renate Lux
STATEMENT OF PROBLEM: Periimplantitis is considered the most important biological complication responsible for late implant failure. The physical chemical properties intrinsic to each material can affect the first step to biofilm development and is an important precursor to the adaptive behavior of pathogenic bacteria species. PURPOSE: The purpose of this in vitro study was to evaluate the effect of 2 commercially available implant abutment materials on the adhesion phase and biofilm formation...
March 23, 2017: Journal of Prosthetic Dentistry
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