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Saliva biology

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https://www.readbyqxmd.com/read/27911430/-clinical-presentations-of-herpes-zoster-ophthalmicus-diagnosis-and-therapy
#1
G M Chernakova, E A Kleshcheva, T B Semenova
: Approximately a quarter of the world's population at some point in life is at risk of developing shingles (Herpes Zoster). In 10-20% of cases the first branch of the trigeminal nerve gets involved (Herpes Zoster Ophthalmicus, HZO). Ophthalmic complications of HZO are able to cause a significant reduction in visual function. AIM: To study and summarize clinical features of HZO (including the rate of complications and their nature) and to determine the relationship between clinical and laboratory data from these patients...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27909455/salivary-testosterone-levels-under-psychological-stress-and-its-relationship-with-rumination-and-five-personality-traits-in-medical-students
#2
Reza Afrisham, Sahar Sadegh-Nejadi, Omid SoliemaniFar, Wesam Kooti, Damoon Ashtary-Larky, Fatima Alamiri, Mohammad Aberomand, Sedigheh Najjar-Asl, Ali Khaneh-Keshi
OBJECTIVE: The purpose of this study was to evaluate the salivary testosterone levels under psychological stress and its relationship with rumination and five personality traits in medical students. METHODS: A total of 58 medical students, who wanted to participate in the final exam, were selected by simple random sampling. Two months before the exam, in the basal conditions, the NEO Inventory short form, and the Emotional Control Questionnaire (ECQ) were completed...
November 2016: Psychiatry Investigation
https://www.readbyqxmd.com/read/27900251/salivary-biomarkers-in-oral-squamous-cell-carcinoma-an-insight
#3
REVIEW
T Radhika, Nadeem Jeddy, S Nithya, R M Muthumeenakshi
Oral cancer refers to the malignancies that occur in the oral cavity, lip and pharynx with 90% of oral cancers being squamous cell carcinomas (OSCC). OSCC has the highest mortality ratio compared to other carcinomas. Although oral cavity is easily accessible, most oral cancers are detected at a later stage leading to lower survival rates. Early detection of OSCC is a key factor in improving the prognosis and survival rate of the patient. Rapid advancement in the field of diagnosis has enabled early diagnosis of many potentially malignant conditions even before its clinical manifestations...
November 2016: Journal of Oral Biology and Craniofacial Research
https://www.readbyqxmd.com/read/27881324/bacterial-adhesion-on-direct-and-indirect-dental-restorative-composite-resins-an-in%C3%A2-vitro-study-on-a-natural-biofilm
#4
Giacomo Derchi, Michele Vano, Antonio Barone, Ugo Covani, Alberto Diaspro, Marco Salerno
STATEMENT OF PROBLEM: Both direct and indirect techniques are used for dental restorations. Which technique should be preferred or whether they are equivalent with respect to bacterial adhesion is unclear. PURPOSE: The purpose of this in vitro study was to determine the affinity of bacterial biofilm to dental restorative composite resins placed directly and indirectly. MATERIAL AND METHODS: Five direct composite resins for restorations (Venus Diamond, Adonis, Optifil, Enamel Plus HRi, Clearfil Majesty Esthetic) and 3 indirect composite resins (Gradia, Estenia, Signum) were selected...
October 27, 2016: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/27879420/saliva-and-serum-protein-exchange-at-the-tooth-enamel-surface
#5
D Heller, E J Helmerhorst, F G Oppenheim
The acquired enamel pellicle is an oral, fluid-derived protein layer that forms on the tooth surface. It is a biologically and clinically important integument that protects teeth against enamel demineralization, and abrasion. Tooth surfaces are exposed to different proteinaceous microenvironments depending on the enamel location. For instance, tooth surfaces close to the gingival sulcus contact serum proteins that emanate via this sulcus, which may impact pellicle composition locally. The aims of this study were to define the major salivary and serum components that adsorb to hydroxyapatite, to study competition among them, and to obtain preliminary evidence in an in vivo saliva/serum pellicle model...
November 22, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/27847745/carbohydrate-electrolyte-drinks-exhibit-risks-for-human-enamel-surface-loss
#6
Mary Anne Sampaio de Melo, Vanara Florêncio Passos, Juliana Paiva Marques Lima, Sérgio Lima Santiago, Lidiany Karla Azevedo Rodrigues
OBJECTIVES: The aim of this investigation was to give insights into the impact of carbohydrate-electrolyte drinks on the likely capacity of enamel surface dissolution and the influence of human saliva exposure as a biological protective factor. MATERIALS AND METHODS: The pH, titratable acidity (TA) to pH 7.0, and buffer capacity (β) of common beverages ingested by patients under physical activity were analyzed. Then, we randomly distributed 50 specimens of human enamel into 5 groups...
November 2016: Restorative Dentistry & Endodontics
https://www.readbyqxmd.com/read/27834029/opiorphin-levels-in-fluids-of-burning-mouth-syndrome-patients-a-case-control-study
#7
Yves Boucher, Adeline Braud, Evelyne Dufour, Scarlette Agbo-Godeau, Vanessa Baaroun, Vianney Descroix, Marie-Thérèse Guinnepain, Marie-Noëlle Ungeheuer, Catherine Ottone, Catherine Rougeot
OBJECTIVES: Idiopathic Burning mouth syndrome (iBMS) is a poorly understood affection characterized by persistent pain in the oral cavity without any clinical or biological abnormality. Opiorphin is a natural inhibitor of enkephalin-inactivating ectopeptidases, mainly produced by salivary glands, that has demonstrated analgesic properties. The objective of the present case-control study was to test the hypothesis of a decrease in opiorphin levels in iBMS patients. MATERIALS AND METHODS: Twenty-one iBMS patients and 21 matched controls subjects were included between 2011 and 2013...
November 10, 2016: Clinical Oral Investigations
https://www.readbyqxmd.com/read/27828988/environmental-dna-from-residual-saliva-for-efficient-noninvasive-genetic-monitoring-of-brown-bears-ursus-arctos
#8
Rachel E Wheat, Jennifer M Allen, Sophie D L Miller, Christopher C Wilmers, Taal Levi
Noninvasive genetic sampling is an important tool in wildlife ecology and management, typically relying on hair snaring or scat sampling techniques, but hair snaring is labor and cost intensive, and scats yield relatively low quality DNA. New approaches utilizing environmental DNA (eDNA) may provide supplementary, cost-effective tools for noninvasive genetic sampling. We tested whether eDNA from residual saliva on partially-consumed Pacific salmon (Oncorhynchus spp.) carcasses might yield suitable DNA quality for noninvasive monitoring of brown bears (Ursus arctos)...
2016: PloS One
https://www.readbyqxmd.com/read/27823669/an-effective-microfluidic-based-liquid-phase-microextraction-device-%C3%AE-lpme-for-extraction-of-non-steroidal-anti-inflammatory-drugs-from-biological-and-environmental-samples
#9
María Ramos-Payan, Santiago Maspoch, Andreu Llobera
In this work, the traditional liquid phase microextraction (LPME) has been miniaturized into a microfluidic device (μLPME) where liquid phase microextraction is combined with an HPLC procedure. This integration enables extraction and determination of acid drugs by μLPME and HPLC, respectively. The analytes selected for the test are five widely used non-steroidal anti-inflammatory drugs (NSAIDs): salicylic acid (SAC), ketoprofen (KTP), naproxen (NAX), diclofenac (DIC) and ibuprofen (IBU). They have successfully been detected in biological (urine and saliva) and environmental (lake and river water) samples with excellent clean up, high extraction efficiency and good enrichment factor under stopped-flow conditions...
November 23, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27822318/discovery-and-replication-of-a-peripheral-tissue-dna-methylation-biosignature-to-augment-a-suicide-prediction-model
#10
Makena L Clive, Marco P Boks, Christiaan H Vinkers, Lauren M Osborne, Jennifer L Payne, Kerry J Ressler, Alicia K Smith, Holly C Wilcox, Zachary Kaminsky
BACKGROUND: Suicide is the second leading cause of death among adolescents in the USA, and rates are rising. Methods to identify individuals at risk are essential for implementing prevention strategies, and the development of a biomarker can potentially improve prediction of suicidal behaviors. Prediction of our previously reported SKA2 biomarker for suicide and PTSD is substantially improved by questionnaires assessing perceived stress or anxiety and is therefore reliant on psychological assessment...
2016: Clinical Epigenetics
https://www.readbyqxmd.com/read/27807840/validation-of-biomarker-proteins-using-reverse-capture-protein-microarrays
#11
Catherine Jozwik, Ofer Eidelman, Joshua Starr, Harvey B Pollard, Meera Srivastava
Genomics has revolutionized large-scale and high-throughput sequencing and has led to the discovery of thousands of new proteins. Protein chip technology is emerging as a miniaturized and highly parallel platform that is suited to rapid, simultaneous screening of large numbers of proteins and the analysis of various protein-binding activities, enzyme substrate relationships, and posttranslational modifications. Specifically, reverse capture protein microarrays provide the most appropriate platform for identifying low-abundance, disease-specific biomarker proteins in a sea of high-abundance proteins from biological fluids such as blood, serum, plasma, saliva, urine, and cerebrospinal fluid as well as tissues and cells obtained by biopsy...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27806018/perceived-discrimination-racial-identity-and-multisystem-stress-response-to-social-evaluative-threat-among-african-american-men-and-women
#12
Todd Lucas, Rhiana Wegner, Jennifer Pierce, Mark A Lumley, Heidemarie K Laurent, Douglas A Granger
OBJECTIVES: Understanding individual differences in the psychobiology of the stress response is critical to grasping how psychosocial factors contribute to racial and ethnic health disparities. However, the ways in which environmentally sensitive biological systems coordinate in response to acute stress is not well understood. We used a social-evaluative stress task to investigate coordination among the autonomic nervous system, hypothalamic-pituitary-adrenal axis, and immune/inflammatory system in a community sample of 85 healthy African American men and women...
November 1, 2016: Psychosomatic Medicine
https://www.readbyqxmd.com/read/27796750/circulating-microrna-profiles-as-liquid-biopsies-for-the-characterization-and-diagnosis-of-fibromyalgia-syndrome
#13
Andrea Masotti, Antonella Baldassarre, Maria Paola Guzzo, Cristina Iannuccelli, Christian Barbato, Manuela Di Franco
This work was aimed at investigating the circulating microRNA (miRNA) profiles in serum and saliva of patients affected by fibromyalgia syndrome (FM), correlating their expression values with clinical and clinimetric parameters and to suggest a mathematical model for the diagnosis of FM. A number of 14 FM patients and sex- and age-matched controls were enrolled in our study. The expression of a panel of 179 miRNAs was evaluated by qPCR. Statistical analyses were performed in order to obtain a mathematical linear model, which could be employed as a supporting tool in the diagnosis of FM...
October 29, 2016: Molecular Neurobiology
https://www.readbyqxmd.com/read/27793146/development-and-validation-of-an-assay-for-measurement-of-leptin-in-pig-saliva
#14
Elizabeth M S Schmidt, Damián Escribano, Silvia Martinez-Subiela, Silvia Martinez-Miró, Fuensanta Hernández, Asta Tvarijonaviciute, José J Cerón, Fernando Tecles
BACKGROUND: Leptin has been measured in human in saliva samples. However, the low leptin concentration found in this biological fluid makes necessary the use of high sensitive methods. To the authors' knowledge, leptin has not been measured in porcine saliva. This study aimed to develop and validate a time-resolved immunofluorometric assay (TR-IFMA) for salivary leptin measurements in pigs, using a species-specific antibody, and to evaluate how salivary leptin changes with body weight, food ingestion, and in experimental models of stress and inflammation...
October 28, 2016: BMC Veterinary Research
https://www.readbyqxmd.com/read/27787811/cell-protection-mechanism-through-autophagy-in-hgfs-s-mitis-co-culture-treated-with-chitlac-nag
#15
Marialucia Gallorini, Viviana di Giacomo, Valentina Di Valerio, Monica Rapino, Domenico Bosco, Andrea Travan, Mara Di Giulio, Roberta Di Pietro, Sergio Paoletti, Amelia Cataldi, Silvia Sancilio
Silver-based products have been proven to be effective in retarding and preventing bacterial growth since ancient times. In the field of restorative dentistry, the use of silver ions/nanoparticles has been explored to counteract bacterial infections, as silver can destroy bacterial cell walls by reacting with membrane proteins. However, it is also cytotoxic towards eukaryotic cells, which are capable of internalizing nanoparticles. In this work, we investigated the biological effects of Chitlac-nAg, a colloidal system based on a modified chitosan (Chitlac), administered for 24-48 h to a co-culture of primary human gingival fibroblasts and Streptococcus mitis in the presence of saliva, developed to mimic the microenvironment of the oral cavity...
December 2016: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/27785693/a-high-resolution-accurate-mass-hram-data-dependent-ms-3-neutral-loss-screening-classification-and-relative-quantitation-methodology-for-carbonyl-compounds-in-saliva
#16
Romel Dator, Andrea Carrà, Laura Maertens, Valeria Guidolin, Peter W Villalta, Silvia Balbo
Reactive carbonyl compounds (RCCs) are ubiquitous in the environment and are generated endogenously as a result of various physiological and pathological processes. These compounds can react with biological molecules inducing deleterious processes believed to be at the basis of their toxic effects. Several of these compounds are implicated in neurotoxic processes, aging disorders, and cancer. Therefore, a method characterizing exposures to these chemicals will provide insights into how they may influence overall health and contribute to disease pathogenesis...
October 26, 2016: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/27770761/pre-exposure-to-faeces-or-saliva-of-triatoma-dimidiata-decreases-parasitemia-in-mice-challenged-with-trypanosoma-cruzi-a-description-of-the-inflammatory-reaction-at-the-inoculation-site
#17
Victor Monteon, Enrique Quen-Rámirez, Veronica Macedo-Reyes, Ruth Lopez, Karla Acosta-Viana, Pamela Pennigton, Angel Ramos-Ligonio
Under natural conditions, Trypanosoma cruzi infection is transmitted to mammals when faeces contaminated with metacyclic trypomastigotes gain access through skin lesions, mucosa or bite wounds. Natural infection of bugs with T. cruzi can vary greatly from less than 1% up to 70%, depending on triatomine species: in the case of Triatoma dimidiata, the percentage of infection is around 30%. In this work uses biological fluids (saliva and faeces) from Triatoma dimidiata to inoculate experimental animals once or multiple times, before inoculation with faeces contaminated with metacyclic trypomastigotes discrete type unit Ia (TcI)...
October 1, 2016: Annals of Parasitology
https://www.readbyqxmd.com/read/27763718/proteomic-and-n-glycoproteomic-quantification-reveal-aberrant-changes-in-the-human-saliva-of-oral-ulcer-patients
#18
Ying Zhang, Xi Wang, Dan Cui, Jun Zhu
The human whole saliva is a vital body fluid to study the physiology and pathology of oral cavity. As a powerful technique for biomarker discovery, mass spectrometry-based proteomic strategy has been introduced to saliva analysis and identified hundreds of proteins and N-glycosylation sites. However, there still lacks of quantitative analysis, which is necessary for biomarker screening and biological researches. In this study, we established an integrated workflow by the combination of stable isotope dimethyl labeling, HILIC enrichment and high resolution mass spectrometry for both quantification of global proteome and N-glycoproteome of human saliva from oral ulcer patients...
October 20, 2016: Proteomics
https://www.readbyqxmd.com/read/27760422/how-we-experience-being-alone-age-differences-in-affective-and-biological-correlates-of-momentary-solitude
#19
Theresa Pauly, Jennifer C Lay, Urs M Nater, Stacey B Scott, Christiane A Hoppmann
BACKGROUND: Spending time alone constitutes a ubiquitous part of our everyday lives. As we get older, alone time increases. Less is known, however, about age differences in the experience of spending time alone (momentary solitude). OBJECTIVES: We examined time-varying associations between momentary solitude, affect quality, and two hypothalamic-pituitary-adrenal axis activity markers [salivary cortisol; dehydroepiandrosterone sulfate (DHEAs)] to better understand the affective and biological correlates of momentary solitude across the adult life span...
October 20, 2016: Gerontology
https://www.readbyqxmd.com/read/27745723/-psychological-processes-of-stress-management-and-neuroendocrine-regulation-in-incarcerated-adolescent-offenders-a-pilot-study
#20
L Guillod, S Habersaat, M Suter, T Jeanneret, C Bertoni, P Stéphan, S Urben
BACKGROUND: Adolescence is a stressful period where important biological, psychological and social changes occur. Adolescents are particularly vulnerable during this developmental period and can use various strategies to deal with daily stress, such as substance use or externalizing behaviors. In previous studies, stress in adolescents with externalizing behaviors was often linked to ineffective cognitive coping strategies (i.e., constructive thinking) and overlooking the biological aspects involved in stress management such as neuroendocrine regulation...
October 10, 2016: L'Encéphale
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