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Beta glucuronidase bacteria

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https://www.readbyqxmd.com/read/26965216/matrix-extension-study-validation-of-the-compact-dry-ec-method-for-enumeration-of-escherichia-coli-and-non-e-coli-coliform-bacteria-in-selected-foods
#1
Shingo Mizuochi, Maria Nelson, Chris Baylis, Becky Green, Keith Jewell, Farinaz Monadjemi, Yi Chen, Yvonne Salfinger, Maria Cristina Fernandez
The Compact Dry "Nissui" EC method, originally certified by the AOAC Research Institute Performance Test Method(SM) program for enumeration of Escherichia coli and non-E. coli coliforms in raw meat products (Performance Tested Method(SM) 110402), has undergone an evaluation to extend the method's claim to cooked chicken, prewashed bagged shredded iceberg lettuce, frozen cod filets, instant nonfat dry milk powder, and pasteurized milk (2% fat). Compact Dry EC is a ready-to-use dry media sheet containing a cold-soluble gelling agent, selective agents, and a chromogenic medium, which are rehydrated by adding 1 mL diluted sample...
March 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/26193312/effects-of-lactofermented-beetroot-juice-alone-or-with-n-nitroso-n-methylurea-on-selected-metabolic-parameters-composition-of-the-microbiota-adhering-to-the-gut-epithelium-and-antioxidant-status-of-rats
#2
Elżbieta Klewicka, Zenon Zduńczyk, Jerzy Juśkiewicz, Robert Klewicki
An objective of this work was to assess the biological activity of beetroot juice (Chrobry variety, Beta vulgaris L. ssp. vulgaris), which was lactofermented by probiotic bacteria Lactobacillus brevis 0944 and Lactobacillus paracasei 0920. The oxidative status of blood serum, kidneys, and liver of rats consuming the fermented beetroot juice were determined. The experimental rats were divided into four groups on diet type: Basal diet, basal diet supplemented with fermented beetroot juice, basal diet and N-nitroso-N-methylurea treatment, and basal diet supplemented with fermented beetroot juice and N-nitroso-N-methylurea treatment...
July 2015: Nutrients
https://www.readbyqxmd.com/read/26131106/distribution-and-gene-mutation-of-enteric-flora-carrying-%C3%AE-glucuronidase-among-patients-with-colorectal-cancer
#3
Yu Li, Xiaome Zhang, Lu Wang, Yuqing Zhou, Jama Suleiman Hassan, Mingcheng Li
OBJECTIVE: To explore the difference of distribution in intestinal flora among colorectal cancer patients and healthy controls and investigate characteristics and changes of sequences in beta-glucuronidase (β-glucuronidase, β-G). METHODS: Bacterial genomic DNA and E. coli DNA in feces were extracted from colorectal cancer patients and healthy controls respectively. Specific primers for β-G gene were designed and amplified by PCR as templates of fecal bacteria genomic DNA and E...
2015: International Journal of Clinical and Experimental Medicine
https://www.readbyqxmd.com/read/26051570/prevention-of-irinotecan-induced-diarrhea-by-probiotics-a-randomized-double-blind-placebo-controlled-pilot-study
#4
RANDOMIZED CONTROLLED TRIAL
Michal Mego, Jozef Chovanec, Iveta Vochyanova-Andrezalova, Peter Konkolovsky, Milada Mikulova, Maria Reckova, Vera Miskovska, Branislav Bystricky, Juraj Beniak, Lenka Medvecova, Adela Lagin, Daniela Svetlovska, Stanislav Spanik, Vladimir Zajac, Jozef Mardiak, Lubos Drgona
PURPOSE: Diarrhea is one of the dose limiting toxicity of irinotecan. SN-38 is main irinotecan metabolite responsible for diarrhea development, which is excreted in glucuronidated form into the intestine. This study aimed to determine the effectiveness of the probiotics in the prevention of irinotecan induced diarrhea due to reduction of intestinal beta-d-glucuronidase activity. METHODS: Between January 2011 and December 2013, 46 patients with colorectal cancer starting a new line of irinotecan based therapy were included...
June 2015: Complementary Therapies in Medicine
https://www.readbyqxmd.com/read/25688562/impediments-to-enhancement-of-cpt-11-anticancer-activity-by-e-coli-directed-beta-glucuronidase-therapy
#5
Yuan-Ting Hsieh, Kai-Chuan Chen, Chiu-Min Cheng, Tian-Lu Cheng, Mi-Hua Tao, Steve R Roffler
CPT-11 is a camptothecin analog used for the clinical treatment of colorectal adenocarcinoma. CPT-11 is converted into the therapeutic anti-cancer agent SN-38 by liver enzymes and can be further metabolized to a non-toxic glucuronide SN-38G, resulting in low SN-38 but high SN-38G concentrations in the circulation. We previously demonstrated that adenoviral expression of membrane-anchored beta-glucuronidase could promote conversion of SN-38G to SN-38 in tumors and increase the anticancer activity of CPT-11. Here, we identified impediments to effective tumor therapy with E...
2015: PloS One
https://www.readbyqxmd.com/read/24459840/beta-glucuronidase-and-beta-glucosidase-activity-in-stool-specimens-of-children-with-inflammatory-bowel-disease
#6
Marta Mroczyńska, Miroslawa Galecka, Patrycja Szachta, Dorota Kamoda, Zdzislawa Libudzisz, Dorota Roszak
The aim of the study was to analyze the differences in the activity of beta-glucuronidase and beta-glucosidase in stool specimens of children with Inflammatory Bowel Diseases (IBD) and healthy subjects. The disease activity was determined according to the PCDAI scale (Crohn disease) and Truelove-Witts scale (Ulcerative colitis). Enzyme activity was determined by spectrophotometry. There was a correlation between the level of beta - glucosidase activity in stool and patient's age in the group of healthy controls, but not in the IBD group...
2013: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/24309132/%C3%AE-glucuronidase-and-%C3%AE-glucosidase-activity-and-human-fecal-water-genotoxicity-in-the-presence-of-probiotic-lactobacilli-and-the-heterocyclic-aromatic-amine-iq-in-vitro
#7
Adriana Nowak, Katarzyna Śliżewska
The aim of the study was to assess the genotoxicity of fecal water (FW) and the activity of fecal enzymes (β-glucuronidase and β-glucosidase) after incubation with 2-amino-3-methyl-3H-imidazo[4,5-f]quinoline (IQ) and probiotic lactobacilli: Lb. casei 0900, Lb. casei 0908, and Lb. paracasei 0919. Our results show that the carcinogen IQ greatly increased FW genotoxicity (up to 16.92 ± 3.03 U/mg) and the activity of fecal enzymes (up to even 1.4 ± 0.16 U/mg) in 15 individuals (children, adults and elderly)...
January 2014: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/23882757/physicochemical-and-biofunctional-properties-of-crab-chitosan-nanoparticles
#8
The Han Nguyen, Hae Soo Kwak, Sang Moo Kim
The physicochemical and biofunctional properties of crab chitosan nanoparticles of two different sizes (Nano A and B) manufactured by dry milling method were evaluated for commercialization. The deacetylation degrees (DD) of Nano A, B and the control chitosan were 90.9, 93.0, and 92.7% respectively whereas their molecular weights (M(w)) were 43.9, 44.7 and 208.8 kDa. The average sizes of the dispersed Nano A, B and the control chitosan in cetyltrimethylammonium chloride were 735.9, 849.4 and 2,382.4 nm, respectively, which were lower than 1441...
August 2013: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/23674271/evaluation-of-the-effect-of-blackcurrant-products-on-gut-microbiota-and-on-markers-of-risk-for-colon-cancer-in-humans
#9
RANDOMIZED CONTROLLED TRIAL
Abdul-Lateef Molan, Zhuojian Liu, Gabriel Plimmer
The purpose of this study was to determine in healthy humans whether First Leaf (FL; composed of blackcurrant extract powder, lactoferrin and lutein) and Cassis Anthomix 30 (CAM30; blackcurrant extract powder) can positively modify the colonic microbiota by enhancing the growth of the beneficial bacteria and inactivating the toxic bacterial enzymes which are known to be involved in colonic carcinogenesis. Thirty healthy adult male and female volunteers were recruited for this study. Fluorescent in situ hybridization was carried out to analyse the populations of fecal microbiota...
March 2014: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/23113683/effects-of-oat-%C3%AE-glucan-and-barley-%C3%AE-glucan-on-fecal-characteristics-intestinal-microflora-and-intestinal-bacterial-metabolites-in-rats
#10
Rui-Ling Shen, Xue-Ya Dang, Ji-Lin Dong, Xin-Zhong Hu
The primary objective was to determine the beneficial effects of oat β-glucan (OG) and barley β-glucan (BG) on gut health. A total of 200 male Sprague-Dawley rats were divided into 5 groups of 40 rats each, control group (CON), low-dose OG-administered group (OGL), high-dose OG-administered group (OGH), low-dose BG-administered group (BGL), and high-dose BG-administered group (BGH). OGL and OGH were administered oat β-glucan by intragastric gavage at a dose of 0.35 g/kg of body weight (BW) and 0.70 g/kg of BW daily for 6 weeks, and BGL and BGH were administered barley β-glucan...
November 14, 2012: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/21745411/antiobesity-and-lipid-lowering-effects-of-bifidobacterium-spp-in-high-fat-diet-induced-obese-rats
#11
Hyang Mi An, Shin Young Park, Do Kyung Lee, Jung Rae Kim, Min Kyeong Cha, Si Won Lee, Hyung Taeck Lim, Kyung Jae Kim, Nam Joo Ha
BACKGROUND: Recent studies have reported the preventive effects of probiotics on obesity. Among commensal bacteria, bifidobacteria is one of the most numerous probiotics in the mammalian gut and are a type of lactic acid bacteria. The aim of this study was to assess the antiobesity and lipid-lowering effects of Bifidobacterium spp. isolated from healthy Korean on high fat diet-induced obese rats. METHODS: Thirty-six male Sprague-Dawley rats were divided into three groups as follows: (1) SD group, fed standard diet; (2) HFD group, fed high fat diet; and (3) HFD-LAB group, fed high fat diet supplemented with LAB supplement (B...
2011: Lipids in Health and Disease
https://www.readbyqxmd.com/read/21515398/impact-of-beta-glucan-on-the-faecal-microbiota-of-polypectomized-patients-a-pilot-study
#12
RANDOMIZED CONTROLLED TRIAL
K Turunen, E Tsouvelakidou, Tz Nomikos, K C Mountzouris, D Karamanolis, J Triantafillidis, A Kyriacou
Beta-glucans are polysaccharides present in the cell walls of higher plants, in the seeds of some cereals, and certain yeasts and fungi also produce them. It is suggested that they exhibit, among many other health benefits, protective effects against carcinogenesis in the colon, but there is not enough human data to support this. The aim of the study was to determine the effect of barley-derived beta-glucan in the gut microbiota of polypectomized patients. Subjects were randomly assigned to consume 125 g of bread per day with beta-glucan (3 g/d), or without (placebo group), for 3 months...
December 2011: Anaerobe
https://www.readbyqxmd.com/read/21031617/the-ability-of-green-tea-to-positively-modulate-key-markers-of-gastrointestinal-function-in-rats
#13
Abdul-Lateef Molan, Zhuojian Liu, Ruby Tiwari
The beneficial effects of selenium-containing green tea (Se-GTE, 1.44 mg selenium/kg dry leaves) and China green tea (CH-GTE, 0.13 mg selenium/kg leaves) on the population size of lactobacilli and bifidobacteria and the activity of two microbial enzymes in the caeca of rats have been investigated. Oral gavage of rats with Se-GTE extract for 6 days resulted in a significant increase in caecal counts of lactobacilli and bifidobacteria (p < 0.05) while significantly reducing the caecal counts of bacteroides and clostridial bacteria...
November 2010: Phytotherapy Research: PTR
https://www.readbyqxmd.com/read/20666985/monitoring-the-colonization-of-sugarcane-and-rice-plants-by-the-endophytic-diazotrophic-bacterium-gluconacetobacter-diazotrophicus-marked-with-gfp-and-gusa-reporter-genes
#14
L F M Rouws, C H S G Meneses, H V Guedes, M S Vidal, J I Baldani, S Schwab
AIMS: To evaluate the colonization process of sugarcane plantlets and hydroponically grown rice seedlings by Gluconacetobacter diazotrophicus strain PAL5 marked with the gusA and gfp reporter genes. METHODS AND RESULTS: Sugarcane plantlets inoculated in vitro with PAL5 carrying the gfp::gusA plasmid pHRGFPGUS did not present green fluorescence, but beta-glucuronidase (GUS)-stained bacteria could be observed inside sugarcane roots. To complement this existing inoculation methodology for micropropagated sugarcane with a more rapid colonization assay, we employed hydroponically grown gnotobiotic rice seedlings to study PAL5-plant interaction...
September 2010: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/20571117/osmotically-induced-synthesis-of-the-dipeptide-n-acetylglutaminylglutamine-amide-is-mediated-by-a-new-pathway-conserved-among-bacteria
#15
Brice Sagot, Marc Gaysinski, Mohamed Mehiri, Jean-Marie Guigonis, Daniel Le Rudulier, Geneviève Alloing
The dipeptide N-acetylglutaminylglutamine amide (NAGGN) was discovered in the bacterium Sinorhizobium meliloti grown at high osmolarity, and subsequently shown to be synthesized and accumulated by a few osmotically challenged bacteria. However, its biosynthetic pathway remained unknown. Recently, two genes, which putatively encode a glutamine amidotransferase and an acetyltransferase and are up-regulated by osmotic stress, were identified in Pseudomonas aeruginosa. In this work, a locus carrying the orthologous genes in S...
July 13, 2010: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/20551652/juxtapapillary-duodenal-diverticula-and-pancreatobiliary-disease
#16
REVIEW
Naoto Egawa, Hajime Anjiki, Kensuke Takuma, Terumi Kamisawa
Juxtapapillary duodenal diverticula (JPD) are observed in around 10-20% of patients undergoing endoscopic retrograde cholangiopancreatography (ERCP). They are acquired extraluminal outpouchings of the duodenal wall through 'locus minoris resistance' and their incidence increases with age. They have been studied mainly with regard to their association with pancreatobiliary disease. Choledocholithiasis is considered to be strongly associated with JPD, but the role of JPD in the development of cholecystolithiasis and pancreatitis is still disputable...
2010: Digestive Surgery
https://www.readbyqxmd.com/read/20459313/infection-specific-activation-of-the-medicago-truncatula-enod11-early-nodulin-gene-promoter-during-actinorhizal-root-nodulation
#17
Sergio Svistoonoff, Mame-Ourèye Sy, Nathalie Diagne, David G Barker, Didier Bogusz, Claudine Franche
The MtEnod11 gene from Medicago truncatula is widely used as an early infection-related molecular marker for endosymbiotic associations involving both rhizobia and arbuscular mycorrhizal fungi. In this article, heterologous expression of the MtEnod11 promoter has been studied in two actinorhizal trees, Casuarina glauca and Allocasuarina verticillata. Transgenic C. glauca and A. verticillata expressing a ProMtEnod11::beta-glucuronidase (gus) fusion were generated and the activation of the transgene investigated in the context of the symbiotic associations with the N-fixing actinomycete Frankia and both endo- and ectomycorrhizal fungi (Glomus intraradices and Pisolithus albus, respectively)...
June 2010: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/20455322/protective-effect-of-proanthocyanidins-on-endotoxin-induced-experimental-periodontitis-in-rats
#18
Jayamathi Govindaraj, Pamela Emmadi, Deepalakshmi, Vijayalakshmi Rajaram, Geetha Prakash, Rengarajulu Puvanakrishnan
The pathogenesis of periodontitis involves anaerobic oral bacteria as well as the host response to infection and several drugs have been developed which can curtail these deleterious effects. Proanthocyanidin, a novel flavanoid extracted from grape seeds, has been shown to provide a significant therapeutic effect on endotoxin (Escherichia coli) induced experimental periodontitis in rats. In this study, protective action of different doses of proanthocyanidins was investigated in blood by assaying the reactive oxygen species such as hydrogen peroxide, superoxide anion, myeloperoxidase and lipid peroxides, lysosomal enzyme activities such as cathepsin B, cathepsin D, beta-glucuronidase and acid phosphatase, nonenzymatic antioxidants such as ascorbic acid, alpha-tocopherol, ceruloplasmin, reduced glutathione and antioxidant enzymes such as catalase, superoxide dismutase, glutathione peroxidase and glutathione-s-transferase...
February 2010: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/20345643/promoter-elements-of-rice-susceptibility-genes-are-bound-and-activated-by-specific-tal-effectors-from-the-bacterial-blight-pathogen-xanthomonas-oryzae-pv-oryzae
#19
Patrick Römer, Sabine Recht, Tina Strauss, Janett Elsaesser, Sebastian Schornack, Jens Boch, Shiping Wang, Thomas Lahaye
*Plant pathogenic bacteria of the genus Xanthomonas inject transcription activator-like effector (TALe) proteins that bind to and activate host promoters, thereby promoting disease or inducing plant defense. TALes bind to corresponding UPT (up-regulated by TALe) promoter boxes via tandemly arranged 34/35-amino acid repeats. Recent studies uncovered the TALe code in which two amino acid residues of each repeat define specific pairing to UPT boxes. *Here we employed the TALe code to predict potential UPT boxes in TALe-induced host promoters and analyzed these via beta-glucuronidase (GUS) reporter and electrophoretic mobility shift assays (EMSA)...
September 2010: New Phytologist
https://www.readbyqxmd.com/read/20217073/characterization-and-validation-of-a-chemiluminescent-assay-based-on-1-2-dioxetanes-for-rapid-detection-of-viable-escherichia-coli
#20
Annette S Bukh, Peter Roslev
[(4-methoxy-4(3-beta-D-galactose-4-chlorophenyl)]spiro[1,2-dioxetane-3-1,3-tricyclo[7.3.1.0(2,7)]tridec-2,7-ene] ("sbeta-Gal 102") and sodium [4-methoxy-4(3-beta-D-glucuronic acid-4-chlorophenyl)]spiro[1,2-dioxetane-3-1,3-tricyclo[7.3.1.0(2,7)]tridec-2,7-ene] ("sbeta-Glucor 102") are carbohydrate-containing 1,2-dioxetane compounds that produce chemiluminescence upon enzymatic hydrolysis by beta-D-galactosidase, and beta-D-glucuronidase, respectively. In this study, we have characterized and validated a sensitive detection principle for viable Escherichia coli based on enzymatic cleavage of sbeta-Gal 102 and sbeta-Glucor 102 ("ColiLight II")...
May 2010: Applied Microbiology and Biotechnology
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